Addgene pICH75366 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pICH75366


Map View

pRS-marker (5353 .. 5372) PvuI (5245) M13/pUC Forward (5137 .. 5159) M13 Forward (5128 .. 5145) EcoRI (5122) KpnI (5114) Acc65I (5110) SmaI (5108) TspMI - XmaI (5106) BamHI (5101) XbaI (5095) AccI (5090) SalI (5089) PstI - SbfI (5087) SphI (5081) M13 Reverse (5042 .. 5058) lac operator M13/pUC Reverse (5023 .. 5045) CAP binding site AseI (4943) BbsI (4877) ApaI (4859) PspOMI (4855) BsaBI * (4387) pVS1 StaA AclI (3784) BsiWI (2823) PmeI (7) pBR322ori-F (249 .. 268) BssSI - BssSαI (281) EcoRV (1029) BlpI (1302) BpmI (1451) PpuMI (1494) Bpu10I (1633) AfeI (1842) MreI - SgrAI (1925) BspQI - SapI (2088) PciI (2204) AgeI (2213) EcoNI (2397) BseRI (2436) BspDI * - ClaI * (2629) NheI (2707) BmtI (2711) pICH75366 5482 bp

Sequence View

PmeI 60 RB T-DNA repeat g t g g t t t a a a c t a t c a g t g t t t g a c a g g a t a t a t t g g c g g g t a a a c c t a a g a g a a a a g a g c a c c a a a t t t g a t a g t c a c a a a c t g t c c t a t a t a a c c g c c c a t t t g g a t t c t c t t t t c t c

120 c g t t t a t t a g a a t a a t c g g a t a t t t a a a a g g g c g t g a a a a g g t t t a t c c g t t c g t c c a t t g c a a a t a a t c t t a t t a g c c t a t a a a t t t t c c c g c a c t t t t c c a a a t a g g c a a g c a g g t a a

180 ori t g t a t g t g c c a a c c a c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t a c a t a c a c g g t t g g t g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a

240 ori c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g

BssSαIBssSI 300 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g

360 ori g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g

420 ori t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c

480 ori t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t

540 ori g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c

600 ori c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t

660 ori c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c

720 ori a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c

780 ori c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a g g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t c c t t c t a g g a a a c t a g a a a a g a t g

840 g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g

900 a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c

960 265 260 255 *FLEDLLIYYK KanR *FLEDLLIYYK t a t a t a t g a g t a a a c t t g g t c t g a c a g c t a a a a c a a t t c a t c c a g t a a a a t a t a a t a t t t a t a t a t a c t c a t t t g a a c c a g a c t g t c g a t t t t g t t a a g t a g g t c a t t t t a t a t t a t a a a

1020 250 245 240 235 IKEWDPKIGLLDFFLEVYQE KanR IKEWDPKIGLLDFFLEVYQE t a t t t t c t c c c a a t c a g g c t t g a t c c c c a g t a a g t c a a a a a a t a g c t c g a c a t a c t g t t c a t a a a a g a g g g t t a g t c c g a a c t a g g g g t c a t t c a g t t t t t t a t c g a g c t g t a t g a c a a g

EcoRV 1080 230 225 220 215 EGIDERISRVCFAIDYWKDA KanR EGIDERISRVCFAIDYWKDA t t c c c c g a t a t c c t c c c t g a t c g a c c g g a c g c a g a a g g c a a t g t c a t a c c a c t t g t c c g c a a g g g g c t a t a g g a g g g a c t a g c t g g c c t g c g t c t t c c g t t a c a g t a t g g t g a a c a g g c g

1140 210 205 200 195 RGSRGLDIFGSVKGDKVFIN KanR RGSRGLDIFGSVKGDKVFIN c c t g c c g c t t c t c c c a a g a t c a a t a a a g c c a c t t a c t t t g c c a t c t t t c a c a a a g a t g t t g g a c g g c g a a g a g g g t t c t a g t t a t t t c g g t g a a t g a a a c g g t a g a a a g t g t t t c t a c a a

1200 190 185 180 175 SDGLDGHSFVLEEEPKETKL KanR SDGLDGHSFVLEEEPKETKL g c t g t c t c c c a g g t c g c c g t g g g a a a a g a c a a g t t c c t c t t c g g g c t t t t c c g t c t t t a a c g a c a g a g g g t c c a g c g g c a c c c t t t t c t g t t c a a g g a g a a g c c c g a a a a g g c a g a a a t t

1260 170 165 160 155 FDYLERPDKFPTDEEWNECD KanR FDYLERPDKFPTDEEWNECD a a a a t c a t a c a g c t c g c g c g g a t c t t t a a a t g g a g t g t c c t c t t c c c a g t t t t c g c a a t c t t t t a g t a t g t c g a g c g c g c c t a g a a a t t t a c c t c a c a g g a g a a g g g t c a a a a g c g t t a g

BlpI 1320 150 145 140 135 VDALDNNLLYDLEALRSDLS KanR VDALDNNLLYDLEALRSDLS c a c a t c g g c c a g a t c g t t a t t c a g t a a g t a a t c c a a t t c g g c t a a g c g g c t g t c t a a g c t g t g t a g c c g g t c t a g c a a t a a g t c a t t c a t t a g g t t a a g c c g a t t c g c c g a c a g a t t c g a

1380 130 125 120 115 NTYPCDSIDISHFLRICEAY KanR NTYPCDSIDISHFLRICEAY a t t c g t a t a g g g a c a a t c c g a t a t g t c g a t g g a g t g a a a g a g c c t g a t g c a c t c c g c a t a t a a g c a t a t c c c t g t t a g g c t a t a c a g c t a c c t c a c t t t c t c g g a c t a c g t g a g g c g t a t

1440 110 105 100 95 LEIIKEPSQEDEYEESCLVG KanR LEIIKEPSQEDEYEESCLVG c a g c t c g a t a a t c t t t t c a g g g c t t t g t t c a t c t t c a t a c t c t t c c g a g c a a a g g a c g c c g t c g a g c t a t t a g a a a a g t c c c g a a a c a a g t a g a a g t a t g a g a a g g c t c g t t t c c t g c g g

PpuMIBpmI 1500 90 85 80 75 DAESMLLNSWGDHREFHLVK KanR DAESMLLNSWGDHREFHLVK a t c g g c c t c a c t c a t g a g c a g a t t g c t c c a g c c a t c a t g c c g t t c a a a g t g c a g g a c c t t t a g c c g g a g t g a g t a c t c g t c t a a c g a g g t c g g t a g t a c g g c a a g t t t c a c g t c c t g g a a

1560 70 65 60 55 PVPLKGELWLMMDKEREVDY KanR PVPLKGELWLMMDKEREVDY t g g a a c a g g c a g c t t t c c t t c c a g c c a t a g c a t c a t g t c c t t t t c c c g t t c c a c a t c a t a a c c t t g t c c g t c g a a a g g a a g g t c g g t a t c g t a g t a c a g g a a a a g g g c a a g g t g t a g t a t

1620 50 45 40 35 TTGKYRSDTMKLYLNENEGV KanR TTGKYRSDTMKLYLNENEGV g g t g g t c c c t t t a t a c c g g c t g t c c g t c a t t t t t a a a t a t a g g t t t t c a t t t t c t c c c a c c c a c c a g g g a a a t a t g g c c g a c a g g c a g t a a a a a t t t a t a t c c a a a a g t a a a a g a g g g t g

Bpu10I 1680 30 25 20 15 LKYVKAPSMGETDKVCRYKE KanR LKYVKAPSMGETDKVCRYKE c a g c t t a t a t a c c t t a g c a g g a g a c a t t c c t t c c g t a t c t t t t a c g c a g c g g t a t t t t t c g t c g a a t a t a t g g a a t c g t c c t c t g t a a g g a a g g c a t a g a a a a t g c g t c g c c a t a a a a a g

1740 10 5 1 ILKKLEPSIRMKAM KanR AmpR promoter ILKKLEPSIRMKAM g a t c a g t t t t t t c a a t t c c g g t g a t a t t c t c a t t t t a g c c a t a c t c t t c c t t t t t c a a t a c t a g t c a a a a a a g t t a a g g c c a c t a t a a g a g t a a a a t c g g t a t g a g a a g g a a a a a g t t a t

1800 AmpR promoter t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t

AfeI 1860 AmpR promoter g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c g a a t t g g c c a g c g c t g c c a t t t t t g g g g t g c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g c t t a a c c g g t c g c g a c g g t a a a a a c c c c a c

1920 a g g c c g t t c g c g g c c g a g g g g c g c a g c c c c t g g g g g g a t g g g a g c t c g g t g c c t t g a t g t t c c g g c a a g c g c c g g c t c c c c g c g t c g g g g a c c c c c c t a c c c t c g a g c c a c g g a a c t a c a

SgrAIMreI 1980 g g g c g c c g g c g g t c g a g t g g c g a c g g c g c g g c t t g t c c g c g c c c t g g t a g a t t g c c t g g c c c c g c g g c c g c c a g c t c a c c g c t g c c g c g c c g a a c a g g c g c g g g a c c a t c t a a c g g a c c g

2040 c g t a g g c c a g c c a t t t t t g a g c g g c c a g c g g c c g c g a t a g g c c g a c g c g a a g c g g c g g g g g c a t c c g g t c g g t a a a a a c t c g c c g g t c g c c g g c g c t a t c c g g c t g c g c t t c g c c g c c c c

BspQISapI 2100 c g t a g g g a g c g c a g c g a c c g a a g g g t a g g c g c t t t t t g c a g c t c t t c g g c t g t g c g c t g g g c a t c c c t c g c g t c g c t g g c t t c c c a t c c g c g a a a a a c g t c g a g a a g c c g a c a c g c g a c c

2160 pVS1 oriV c c a g a c a g t t a t g c a c a g g c c a g g c g g g t t t t a a g a g t t t t a a t a a g t t t t a a a g a g t t t g g t c t g t c a a t a c g t g t c c g g t c c g c c c a a a a t t c t c a a a a t t a t t c a a a a t t t c t c a a a

AgeIPciI 2220 pVS1 oriV t a g g c g g a a a a a t c g c c t t t t t t c t c t t t t a t a t c a g t c a c t t a c a t g t g t g a c c g g t t c a t c c g c c t t t t t a g c g g a a a a a a g a g a a a a t a t a g t c a g t g a a t g t a c a c a c t g g c c a a g

2280 pVS1 oriV c c a a t g t a c g g c t t t g g g t t c c c a a t g t a c g g g t t c c g g t t c c c a a t g t a c g g c t t t g g g g g t t a c a t g c c g a a a c c c a a g g g t t a c a t g c c c a a g g c c a a g g g t t a c a t g c c g a a a c c c

2340 pVS1 oriV t t c c c a a t g t a c g t g c t a t c c a c a g g a a a g a a g c t t t t t c g a c c t t t t t c c c c t g c t a g g a a g g g t t a c a t g c a c g a t a g g t g t c c t t t c t t c g a a a a a g c t g g a a a a a g g g g a c g a t c c

EcoNI 2400 355 350 *SGASAEGEI pVS1 RepA *SGASAEGEI g c a a t t t g c c c t a g c a t c t g c t c c g t a c a t t a g g a a c c g g c g g a t g c t t c g c c c t c g a t c c g t t a a a c g g g a t c g t a g a c g a g g c a t g t a a t c c t t g g c c g c c t a c g a a g c g g g a g c t a g

BseRI 2460 345 340 335 330 LNRYRMVLIPGAQGAEEKLD pVS1 RepA LNRYRMVLIPGAQGAEEKLD a g g t t g c g g t a g c g c a t g a c t a g g a t c g g g c c a g c c t g c c c c g c c t c c t c c t t c a a a t c g t c c a a c g c c a t c g c g t a c t g a t c c t a g c c c g g t c g g a c g g g g c g g a g g a g g a a g t t t a g c

2520 325 320 315 310 YEPLDNSGILKRVTFCFKKF pVS1 RepA YEPLDNSGILKRVTFCFKKF t a c t c c g g c a g g t c a t t t g a c c c g a t c a g c t t g c g c a c g g t g a a a c a g a a c t t c t t g a a c a t g a g g c c g t c c a g t a a a c t g g g c t a g t c g a a c g c g t g c c a c t t t g t c t t g a a g a a c t t g

2580 305 300 295 290 EGASGSREYITKFLWRAEAK pVS1 RepA EGASGSREYITKFLWRAEAK t c t c c g g c g c t g c c a c t g c g t t c g t a g a t c g t c t t g a a c a a c c a t c t g g c t t c t g c c t t g a g a g g c c g c g a c g g t g a c g c a a g c a t c t a g c a g a a c t t g t t g g t a g a c c g a a g a c g g a a c

BspDI*ClaI* 2640 285 280 275 270 GAARRALRYLFRGIGPDILF pVS1 RepA GAARRALRYLFRGIGPDILF c c t g c g g c g c g g c g t g c c a g g c g g t a g a g a a a a c g g c c g a t g c c g g g a t c g a t c a a a a a g g g a c g c c g c g c c g c a c g g t c c g c c a t c t c t t t t g c c g g c t a c g g c c c t a g c t a g t t t t t c

2700 265 260 255 250 YDPHVTLVDPNKGETIERYM pVS1 RepA YDPHVTLVDPNKGETIERYM t a a t c g g g g t g a a c c g t c a g c a c g t c c g g g t t c t t g c c t t c t g t g a t c t c g c g g t a c a t c a t t a g c c c c a c t t g g c a g t c g t g c a g g c c c a a g a a c g g a a g a c a c t a g a g c g c c a t g t a g

BmtINheI 2760 245 240 235 230 WDALEIEIYEPRGTESKVIK pVS1 RepA WDALEIEIYEPRGTESKVIK c a a t c a g c t a g c t c g a t c t c g a t g t a c t c c g g c c g c c c g g t t t c g c t c t t t a c g a t c t t g g t t a g t c g a t c g a g c t a g a g c t a c a t g a g g c c g g c g g g c c a a a g c g a g a a a t g c t a g a a c

2820 225 220 215 210 YRSILAEGESVTVLRGNKAK pVS1 RepA YRSILAEGESVTVLRGNKAK t a g c g g c t a a t c a a g g c t t c a c c c t c g g a t a c c g t c a c c a g g c g g c c g t t c t t g g c c t t c a t c g c c g a t t a g t t c c g a a g t g g g a g c c t a t g g c a g t g g t c c g c c g g c a a g a a c c g g a a g

BsiWI 2880 205 200 195 190 KTRQMAVHTTNLRICTEVLD pVS1 RepA KTRQMAVHTTNLRICTEVLD t t c g t a c g c t g c a t g g c a a c g t g c g t g g t g t t t a a c c g a a t g c a g g t t t c t a c c a g g t c g a a g c a t g c g a c g t a c c g t t g c a c g c a c c a c a a a t t g g c t t a c g t c c a a a g a t g g t c c a g c

2940 185 180 175 170 DKQKGGDARRCFKLVDAVHP pVS1 RepA DKQKGGDARRCFKLVDAVHP t c t t t c t g c t t t c c g c c a t c g g c t c g c c g g c a g a a c t t g a g t a c g t c c g c a a c g t g t g g a a g a a a g a c g a a a g g c g g t a g c c g a g c g g c c g t c t t g a a c t c a t g c a g g c g t t g c a c a c c t

3000 165 160 155 150 RFVRGPKDGKGERYRNMSET pVS1 RepA RFVRGPKDGKGERYRNMSET c g g a a c a c g c g g c c g g g c t t g t c t c c c t t c c c t t c c c g g t a t c g g t t c a t g g a t t c g g t t g c c t t g t g c g c c g g c c c g a a c a g a g g g a a g g g a a g g g c c a t a g c c a a g t a c c t a a g c c a a

3060 145 140 135 130 LHSVAMLVLDYDWVSAMGAP pVS1 RepA MGNRHQYQVVIPHTGHAGR LHSVAMLVLDYDWVSAMGAP a g a t g g g a a a c c g c c a t c a g t a c c a g g t c g t a a t c c c a c a c a c t g g c c a t g c c g g c c g g c t c t a c c c t t t g g c g g t a g t c a t g g t c c a g c a t t a g g g t g t g t g a c c g g t a c g g c c g g c c g

3120 125 120 115 110 GASVEVHGDPLEYRIVEGAR pVS1 RepA PCGNLYVPVWKLVADHLASS GASVEVHGDPLEYRIVEGAR c c t g c g g a a a c c t c t a c g t g c c c g t c t g g a a g c t c g t a g c g g a t c a c c t c g c c a g c t c g t g g a c g c c t t t g g a g a t g c a c g g g c a g a c c t t c g a g c a t c g c c t a g t g g a g c g g t c g a g c a

3180 105 100 95 90 RDRKSLRFVAVDMISRSDRT pVS1 RepA SVTLRQTENGHVHDAATIAG RDRKSLRFVAVDMISRSDRT c g g t c a c g c t t c g a c a g a c g g a a a a c g g c c a c g t c c a t g a t g c t g c g a c t a t c g c g g g t g g c c a g t g c g a a g c t g t c t g c c t t t t g c c g g t g c a g g t a c t a c g a c g c t g a t a g c g c c c a c

3240 85 80 75 70 GVDYLMPVFFDPQEDGKPPK pVS1 RepA AHVIEHRNEKIWLLVALGRL GVDYLMPVFFDPQEDGKPPK c c c a c g t c a t a g a g c a t c g g a a c g a a a a a a t c t g g t t g c t c g t c g c c c t t g g g c g g c t t c g g g t g c a g t a t c t c g t a g c c t t g c t t t t t t a g a c c a a c g a g c a g c g g g a a c c c g c c g a a g

3300 65 60 55 50 RISPAGAAPPQRSEKRIRDA pVS1 RepA PNRRRTGCRRLPGFFADSIS RISPAGAAPPQRSEKRIRDA c t a a t c g a c g g c g c a c c g g c t g c c g g c g g t t g c c g g g a t t c t t t g c g g a t t c g a t c a g c g g a t t a g c t g c c g c g t g g c c g a c g g c c g c c a a c g g c c c t a a g a a a c g c c t a a g c t a g t c g c

3360 45 40 35 30 AAQWSEGPRAEAEIRQRQAA pVS1 RepA GRLPRFTGACFCLDALPLGG AAQWSEGPRAEAEIRQRQAA g c c g c t t g c c a c g a t t c a c c g g g g c g t g c t t c t g c c t c g a t g c g t t g c c g c t g g g c g g c c c g g c g a a c g g t g c t a a g t g g c c c c g c a c g a a g a c g g a g c t a c g c a a c g g c g a c c c g c c g g

3420 25 20 15 10 QAAKLKEVLDDGLAAGIQVP pVS1 RepA LRGLQLLHQVITQRRADLYR QAAKLKEVLDDGLAAGIQVP t g c g c g g c c t t c a a c t t c t c c a c c a g g t c a t c a c c c a g c g c c g c g c c g a t t t g t a c c g g g a c g c g c c g g a a g t t g a a g a g g t g g t c c a g t a g t g g g t c g c g g c g c g g c t a a a c a t g g c c c

3480 5 GSPKRG pVS1 RepA AGWFATVTPIPRAWGFQCHC GSPKRGDRRNRPSPPELAM c c g g a t g g t t t g c g a c c g t c a c g c c g a t t c c t c g g g c t t g g g g g t t c c a g t g c c a t t g c a g g c c t a c c a a a c g c t g g c a g t g c g g c t a a g g a g c c c g a a c c c c c a a g g t c a c g g t a a c g t

3540 RAGRQPSRLRLANRPFLHTW g g g c c g g c a g a c a a c c c a g c c g c t t a c g c c t g g c c a a c c g c c c g t t c c t c c a c a c a t g g g c c c g g c c g t c t g t t g g g t c g g c g a a t g c g g a c c g g t t g g c g g g c a a g g a g g t g t g t a c c c

3600 GIPRRRCLVVLDFPCRLL* g c a t t c c a c g g c g t c g g t g c c t g g t t g t t c t t g a t t t t c c a t g c c g c c t c c t t t a g c c g c c g t a a g g t g c c g c a g c c a c g g a c c a a c a a g a a c t a a a a g g t a c g g c g g a g g a a a t c g g c g

3660 t a a a a t t c a t c t a c t c a t t t a t t c a t t t g c t c a t t t a c t c t g g t a g c t g c g c g a t g t a t t a t t t t a a g t a g a t g a g t a a a t a a g t a a a c g a g t a a a t g a g a c c a t c g a c g c g c t a c a t a a

3720 c a g a t a g c a g c t c g g t a a t g g t c t t g c c t t g g c g t a c c g c g t a c a t c t t c a g c t t g g t g t g t c t a t c g t c g a g c c a t t a c c a g a a c g g a a c c g c a t g g c g c a t g t a g a a g t c g a a c c a c a

3780 g a t c c t c c g c c g g c a a c t g a a a g t t g a c c c g c t t c a t g g c t g g c g t g t c t g c c a g g c t g g c t a g g a g g c g g c c g t t g a c t t t c a a c t g g g c g a a g t a c c g a c c g c a c a g a c g g t c c g a c c

AclI 3840 c c a a c g t t g c a g c c t t g c t g c t g c g t g c g c t c g g a c g g c c g g c a c t t a g c g t g t t t g t g c g g t t g c a a c g t c g g a a c g a c g a c g c a c g c g a g c c t g c c g g c c g t g a a t c g c a c a a a c a c g

3900 210 205 *ILKSKIEAA pVS1 StaA *ILKSKIEAA t t t t g c t c a t t t t c t c t t t a c c t c a t t a a c t c a a a t g a g t t t t g a t t t a a t t t c a g c g g c a a a a c g a g t a a a a g a g a a a t g g a g t a a t t g a g t t t a c t c a a a a c t a a a t t a a a g t c g c c g

3960 200 195 190 185 LAQVERAADGEPESELVTTG pVS1 StaA LAQVERAADGEPESELVTTG c a g c g c c t g g a c c t c g c g g g c a g c g t c g c c c t c g g g t t c t g a t t c a a g a a c g g t t g t g c c g t c g c g g a c c t g g a g c g c c c g t c g c a g c g g g a g c c c a a g a c t a a g t t c t t g c c a a c a c g g

4020 180 175 170 165 AAATGPYSVRQTIRSELIPL pVS1 StaA AAATGPYSVRQTIRSELIPL g g c g g c g g c a g t g c c t g g g t a g c t c a c g c g c t g c g t g a t a c g g g a c t c a a g a a t g g g c a g c c g c c g c c g t c a c g g a c c c a t c g a g t g c g c g a c g c a c t a t g c c c t g a g t t c t t a c c c g t c

4080 160 155 150 145 EYGALAEAVEGGIRTGKIAR pVS1 StaA EYGALAEAVEGGIRTGKIAR c t c g t a c c c g g c c a g c g c c t c g g c a a c c t c a c c g c c g a t g c g c g t g c c t t t g a t c g c c c g g a g c a t g g g c c g g t c g c g g a g c c g t t g g a g t g g c g g c t a c g c g c a c g g a a a c t a g c g g g c

4140 140 135 130 125 SVVFAAQLRGDTVEIRQKVL pVS1 StaA SVVFAAQLRGDTVEIRQKVL c g a c a c g a c a a a g g c c g c t t g t a g c c t t c c a t c c g t g a c c t c a a t g c g c t g c t t a a c c a g g c t g t g c t g t t t c c g g c g a a c a t c g g a a g g t a g g c a c t g g a g t t a c g c g a c g a a t t g g t c

4200 120 115 110 105 EVLDATAWIDYPSPQVPILV pVS1 StaA EVLDATAWIDYPSPQVPILV c t c c a c c a g g t c g g c g g t g g c c c a t a t g t c g t a a g g g c t t g g c t g c a c c g g a a t c a g c a c g a g g t g g t c c a g c c g c c a c c g g g t a t a c a g c a t t c c c g a a c c g a c g t g g c c t t a g t c g t g

4260 100 95 90 85 FDAAKIASVALDAAQPAGDI pVS1 StaA FDAAKIASVALDAAQPAGDI g a a g t c g g c t g c c t t g a t c g c g g a c a c a g c c a a g t c c g c c g c c t g g g g c g c t c c g t c g a t c t t c a g c c g a c g g a a c t a g c g c c t g t g t c g g t t c a g g c g g c g g a c c c c g c g a g g c a g c t a

4320 80 75 70 65 VVFDRRGIAKVDRDITPRDI pVS1 StaA VVFDRRGIAKVDRDITPRDI c a c t a c g a a g t c g c g c c g g c c g a t g g c c t t c a c g t c g c g g t c a a t c g t c g g g c g g t c g a t g t g a t g c t t c a g c g c g g c c g g c t a c c g g a a g t g c a g c g c c a g t t a g c a g c c c g c c a g c t a

4380 60 55 50 45 GVVTLPQDERVAAWDRASGQ pVS1 StaA GVVTLPQDERVAAWDRASGQ g c c g a c a a c g g t t a g c g g t t g a t c t t c c c g c a c g g c c g c c c a a t c g c g g g c a c t g c c c t g c g g c t g t t g c c a a t c g c c a a c t a g a a g g g c g t g c c g g c g g g t t a g c g c c c g t g a c g g g a c

BsaBI* 4440 40 35 30 25 PDSDVLLVDAGALQLARALH pVS1 StaA PDSDVLLVDAGALQLARALH g g g a t c g g a a t c g a c t a a c a g a a c a t c g g c c c c g g c g a g t t g c a g g g c g c g g g c t a g a t g c c c t a g c c t t a g c t g a t t g t c t t g t a g c c g g g g c c g c t c a a c g t c c c g c g c c c g a t c t a c

4500 20 15 10 5 1 TAITTKGSGGKQNLVAIVKM pVS1 StaA TAITTKGSGGKQNLVAIVKM g g t t g c g a t g g t c g t c t t g c c t g a c c c g c c t t t c t g g t t a a g t a c a g c g a t a a c c t t c a t c c a a c g c t a c c a g c a g a a c g g a c t g g g c g g a a a g a c c a a t t c a t g t c g c t a t t g g a a g t a

4560 g c g t t c c c c t t g c g t a t t t g t t t a t t t a c t c a t c g c a t c a t a t a c g c a g c g a c c g c a t g a c g c a a g g g g a a c g c a t a a a c a a a t a a a t g a g t a g c g t a g t a t a t g c g t c g c t g g c g t a c t

4620 c g c a a g c t g t t t t a c t c a a a t a c a c a t c a c c t t t t t a g a c g g c g g c g c t c g g t t t c t t c a g c g t t c g a c a a a a t g a g t t t a t g t g t a g t g g a a a a a t c t g c c g c c g c g a g c c a a a g a a g t

4680 g c g g c c a a g c t g g c c g g c c a g g c c g c c a g c t t g g c a t c a g a c a a a c c g g c c a g g a t t t c a c g c c g g t t c g a c c g g c c g g t c c g g c g g t c g a a c c g t a g t c t g t t t g g c c g g t c c t a a a g t

4740 t g c a g c c g c a c g g c t g a t g g g c t g c c t g t a t c g a g t g g t g a t t t t g t g c c g a g c t g c c g g a c g t c g g c g t g c c g a c t a c c c g a c g g a c a t a g c t c a c c a c t a a a a c a c g g c t c g a c g g c c

4800 LB T-DNA repeat t c g g g g a g c t g t t g g c t g g c t g g t g g c a g g a t a t a t t g t g g t g t a a a c a a a t t g a c g c t t a g c c c c t c g a c a a c c g a c c g a c c a c c g t c c t a t a t a a c a c c a c a t t t g t t t a a c t g c g a a

PspOMIApaI 4860 a g a c a a c t t a a t a a c a c a t t g c g g a c g t t t t t a a t g t a c t g g g g t g g a t g c a g t g g g c c c t c t g t t g a a t t a t t g t g t a a c g c c t g c a a a a a t t a c a t g a c c c c a c c t a c g t c a c c c g g g

BbsI 4920 c a c t c t g t g g a a g a c a a c a g a t g a g a c c g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g t g a g a c a c c t t c t g t t g t c t a c t c t g g c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t

AseI 4980 CAP binding site lac promoter g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a

5040 -35 -10 lac promoter lac operator A G C G G A T A A C A A T T T C A C M13/pUC Reverse t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g

XbaISphISbfIPstISalIAccI 5100 1 5 10 15 MTMITPSLHACRSTLE lacZ α M13 rev MCS A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c t t g c a t g c c t g c a g g t c g a c t c t a g a t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g a a c g t a c g g a c g t c c a g c t g a g a t c t

EcoRIBamHITspMIXmaISmaIAcc65IKpnI 5160 20 25 30 35 DPRVPSSNSLAVVLQRRDWE lacZ α MCS M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward g g a t c c c c g g g t a c c g a g c t c g a a t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a c c t a g g g g c c c a t g g c t c g a g c t t a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t

5220 40 45 50 55 NPGVTQLNRLAAHPPFASWR lacZ α a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c

PvuI 5280 60 65 70 75 NSEEARTDRPSQQLRSLNGE lacZ α t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t

5340 80 85 90 95 WRLMRYFLLTHLCGISHRIW lacZ α a t g g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g t a c c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c

5400 100 105 CTLSTICSDAA* lacZ α A T G T T A G A C G A G A C T A C G G C pRS-marker g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g

5460 a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g

5482 t g t g a g g t c t c a g g g a c a c g a a a c a c t c c a g a g t c c c t g t g c t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.