Addgene pGEX Socs5-SH2 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pGEX Socs5-SH2


Map View

BtgI (5254) Bsu36I (5146) M13/pUC Forward (4937 .. 4959) M13 Forward (4928 .. 4945) M13 Reverse (4896 .. 4912) lac operator PluTI (4696) SfoI (4694) NarI * (4693) KasI (4692) EcoRV (4503) BssHII (4464) BstEII (4234) MluI (4053) LacI-R (3722 .. 3741) BstAPI (3736) PflMI (3635) L4440 (3535 .. 3552) pBR322ori-F (3282 .. 3301) AlwNI (3028) AhdI (2549) BfuAI - BspMI (62) lac operator EcoNI (268) MscI (465) BstBI (655) SwaI (685) pGEX 5' (869 .. 891) PasI (941) BamHI (945) Bpu10I (1032) SphI (1101) BseRI (1154) PsiI (1195) EcoRI (1264) HindIII (1280) EagI - NotI (1345) pGEX 3' (1404 .. 1426) PflFI - Tth111I (1524) BsaAI (1531) pBRforEco (1571 .. 1589) ZraI (1628) AatII (1630) Amp-R (1980 .. 1999) PstI (2307) BsaI (2483) pGEX Socs5-SH2 5354 bp

Sequence View

60 150 155 160 165 TLSTARCTNASGVRQPSEAV lacZ α TLSTARCTNASGVRQPSEAV a c g t t a t c g a c t g c a c g g t g c a c c a a t g c t t c t g g c g t c a g g c a g c c a t c g g a a g c t g t g t g c a a t a g c t g a c g t g c c a c g t g g t t a c g a a g a c c g c a g t c c g t c g g t a g c c t t c g a c a c

BspMIBfuAI 120 170 175 VWLCRS* lacZ α VWLCRS* g t a t g g c t g t g c a g g t c g t a a a t c a c t g c a t a a t t c g t g t c g c t c a a g g c g c a c t c c c g t c a t a c c g a c a c g t c c a g c a t t t a g t g a c g t a t t a a g c a c a g c g a g t t c c g c g t g a g g g c a

180 t c t g g a t a a t g t t t t t t g c g c c g a c a t c a t a a c g g t t c t g g c a a a t a t t c t g a a a t g a g c a g a c c t a t t a c a a a a a a c g c g g c t g t a g t a t t g c c a a g a c c g t t t a t a a g a c t t t a c t c g

240 -35 -10 tac promoter lac operator t g t t g a c a a t t a a t c a t c g g c t c g t a t a a t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a a c a a c t g t t a a t t a g t a g c c g a g c a t a t t a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t

EcoNI 300 1 5 10 MSPILGYWKIKGLV GST MSPILGYWKIKGLV c a c a g g a a a c a g t a t t c a t g t c c c c t a t a c t a g g t t a t t g g a a a a t t a a g g g c c t t g t g c g t g t c c t t t g t c a t a a g t a c a g g g g a t a t g a t c c a a t a a c c t t t t a a t t c c c g g a a c a c g

360 15 20 25 30 QPTRLLLEYLEEKYEEHLYE GST QPTRLLLEYLEEKYEEHLYE a a c c c a c t c g a c t t c t t t t g g a a t a t c t t g a a g a a a a a t a t g a a g a g c a t t t g t a t g a g c t t g g g t g a g c t g a a g a a a a c c t t a t a g a a c t t c t t t t t a t a c t t c t c g t a a a c a t a c t c g

420 35 40 45 50 RDEGDKWRNKKFELGLEFPN GST RDEGDKWRNKKFELGLEFPN g c g a t g a a g g t g a t a a a t g g c g a a a c a a a a a g t t t g a a t t g g g t t t g g a g t t t c c c a a t c c g c t a c t t c c a c t a t t t a c c g c t t t g t t t t t c a a a c t t a a c c c a a a c c t c a a a g g g t t a g

MscI 480 55 60 65 70 LPYYIDGDVKLTQSMAIIRY GST LPYYIDGDVKLTQSMAIIRY t t c c t t a t t a t a t t g a t g g t g a t g t t a a a t t a a c a c a g t c t a t g g c c a t c a t a c g t t a t a a a g g a a t a a t a t a a c t a c c a c t a c a a t t t a a t t g t g t c a g a t a c c g g t a g t a t g c a a t a t

540 75 80 85 90 IADKHNMLGGCPKERAEISM GST IADKHNMLGGCPKERAEISM t a g c t g a c a a g c a c a a c a t g t t g g g t g g t t g t c c a a a a g a g c g t g c a g a g a t t t c a a t g c a t c g a c t g t t c g t g t t g t a c a a c c c a c c a a c a g g t t t t c t c g c a c g t c t c t a a a g t t a c g

600 95 100 105 110 LEGAVLDIRYGVSRIAYSKD GST LEGAVLDIRYGVSRIAYSKD t t g a a g g a g c g g t t t t g g a t a t t a g a t a c g g t g t t t c g a g a a t t g c a t a t a g t a a a g a c t a a c t t c c t c g c c a a a a c c t a t a a t c t a t g c c a c a a a g c t c t t a a c g t a t a t c a t t t c t g a

BstBI 660 115 120 125 130 FETLKVDFLSKLPEMLKMFE GST FETLKVDFLSKLPEMLKMFE t t g a a a c t c t c a a a g t t g a t t t t c t t a g c a a g c t a c c t g a a a t g c t g a a a a t g t t c g a a g a a c t t t g a g a g t t t c a a c t a a a a g a a t c g t t c g a t g g a c t t t a c g a c t t t t a c a a g c t t c

SwaI 720 135 140 145 150 DRLCHKTYLNGDHVTHPDFM GST DRLCHKTYLNGDHVTHPDFM a t c g t t t a t g t c a t a a a a c a t a t t t a a a t g g t g a t c a t g t a a c c c a t c c t g a c t t c a t g t t a g c a a a t a c a g t a t t t t g t a t a a a t t t a c c a c t a g t a c a t t g g g t a g g a c t g a a g t a c a

780 155 160 165 170 LYDALDVVLYMDPMCLDAFP GST LYDALDVVLYMDPMCLDAFP t g t a t g a c g c t c t t g a t g t t g t t t t a t a c a t g g a c c c a a t g t g c c t g g a t g c g t t c c c a a a c a t a c t g c g a g a a c t a c a a c a a a a t a t g t a c c t g g g t t a c a c g g a c c t a c g c a a g g g t t

840 175 180 185 190 KLVCFKKRIEAIPQIDKYLK GST KLVCFKKRIEAIPQIDKYLK a a t t a g t t t g t t t t a a a a a a c g t a t t g a a g c t a t c c c a c a a a t t g a t a a g t a c t t g a a a t t t a a t c a a a c a a a a t t t t t t g c a t a a c t t c g a t a g g g t g t t t a a c t a t t c a t g a a c t t t a

900 195 200 205 210 SSKYIAWPLQGWQATFGGGD GST G G G C T G G C A A G C C A C G T T T G G T G pGEX 5' SSKYIAWPLQGWQATFGGGD c c a g c a a g t a t a t a g c a t g g c c t t t g c a g g g c t g g c a a g c c a c g t t t g g t g g t g g c g a c c g g t c g t t c a t a t a t c g t a c c g g a a a c g t c c c g a c c g t t c g g t g c a a a c c a c c a c c g c t g g

BamHIPasI 960 215 HPPK SD 1 5 LEVLFQGP (in frame with HRV 3C site) LGSGNP GST HRV 3C site HPPKSDLEVLFQGPLGSGNP a t c c t c c a a a a t c g g a t c t g g a a g t t c t g t t c c a g g g g c c c c t g g g a t c c g g g a a t c c c t t a g g a g g t t t t a g c c t a g a c c t t c a a g a c a a g g t c c c c g g g g a c c c t a g g c c c t t a g g g a

1020 (in frame with HRV 3C site) CYWGVMDRYEAEALLEGKPE CYWGVMDRYEAEALLEGKPE g t t a c t g g g g a g t g a t g g a c c g t t a t g a a g c a g a a g c c c t t c t c g a a g g g a a a c c t g a a g c a a t g a c c c c t c a c t a c c t g g c a a t a c t t c g t c t t c g g g a a g a g c t t c c c t t t g g a c t t c

Bpu10I 1080 (in frame with HRV 3C site) GTFLLRDSAQEDYLFSVSFR GTFLLRDSAQEDYLFSVSFR g c a c g t t t t t g c t c a g g g a c t c t g c g c a a g a g g a c t a c c t c t t c t c t g t g a g c t t c c g c c c g t g c a a a a a c g a g t c c c t g a g a c g c g t t c t c c t g a t g g a g a a g a g a c a c t c g a a g g c g g

SphI 1140 (in frame with HRV 3C site) RYNRSLHARIEQWNHNFSFD RYNRSLHARIEQWNHNFSFD g c t a c a a c a g a t c c c t g c a t g c c c g a a t t g a g c a g t g g a a t c a c a a c t t t a g t t t c g a c g c g a t g t t g t c t a g g g a c g t a c g g g c t t a a c t c g t c a c c t t a g t g t t g a a a t c a a a g c t g c

PsiIBseRI 1200 (in frame with HRV 3C site) AHDPCVFHSSTVTGLLEHYK AHDPCVFHSSTVTGLLEHYK c c c a t g a c c c g t g t g t a t t t c a c t c c t c c a c t g t a a c g g g a c t t t t a g a a c a t t a t a a a g g g g t a c t g g g c a c a c a t a a a g t g a g g a g g t g a c a t t g c c c t g a a a a t c t t g t a a t a t t t c

1260 (in frame with HRV 3C site) DPSSCMFFEPLLTISLNMTF DPSSCMFFEPLLTISLNMTF a t c c c a g t t c g t g c a t g t t t t t t g a a c c a t t g c t t a c t a t a t c a c t a a a t a t g a c t t t c c t a g g g t c a a g c a c g t a c a a a a a a c t t g g t a a c g a a t g a t a t a g t g a t t t a t a c t g a a a g g

HindIIIEcoRI 1320 (in frame with HRV 3C site) PGIHRDASLLGG 1 5 GLNDIFEA AviTag™ PGIHRDASLLGGGLNDIFEA c t g g a a t t c a t c g t g a c g c a a g c t t g c t t g g t g g c g g t c t g a a c g a c a t c t t c g a g g c t c g a c c t t a a g t a g c a c t g c g t t c g a a c g a a c c a c c g c c a g a c t t g c t g t a g a a g c t c c g a g

EagINotI 1380 10 15 QKIEWHE * AviTag™ QKIEWHE* a g a a a a t c g a a t g g c a c g a a t a a g c g g c c g c a t c g t g a c t g a c t g a c g a t c t g c c t c g c g t c t t t t a g c t t a c c g t g c t t a t t c g c c g g c g t a g c a c t g a c t g a c t g c t a g a c g g a g c g c

1440 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a

1500 t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g

BsaAITth111IPflFI 1560 g g g t g t c g g g g c g c a g c c a t g a c c c a g t c a c g t a g c g a t a g c g g a g t g t a t a a t t c t t g a c c c a c a g c c c c g c g t c g g t a c t g g g t c a g t g c a t c g c t a t c g c c t c a c a t a t t a a g a a c t

1620 C G G A G C A C T A T G C G G A T A A pBRforEco a g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a

ZraIAatII 1680 AmpR promoter t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a

1740 AmpR promoter t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t

1800 1 5 10 signal sequence MSIQHFRVALIPF AmpR promoter AmpR MSIQHFRVALIPF t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a

1860 15 20 signal sequence FAAFCLPVFA 25 30 HPETLVKVKD AmpR FAAFCLPVFAHPETLVKVKD t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a

1920 35 40 45 50 AEDQLGARVGYIELDLNSGK AmpR AEDQLGARVGYIELDLNSGK g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c

1980 55 60 65 70 ILESFRPEERFPMMSTFKVL AmpR C Amp-R ILESFRPEERFPMMSTFKVL a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c

2040 75 80 85 90 LCGAVLSRVDAGQEQLGRRI AmpR G A T A C A C C G C G C C A T A A T A Amp-R LCGAVLSRVDAGQEQLGRRI c t a t g t g g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a g a t a c a c c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t

2100 95 100 105 110 HYSQNDLVEYSPVTEKHLTD AmpR HYSQNDLVEYSPVTEKHLTD c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a

2160 115 120 125 130 GMTVRELCSAAITMSDNTAA AmpR GMTVRELCSAAITMSDNTAA g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g

2220 135 140 145 150 NLLLTTIGGPKELTAFLHNM AmpR NLLLTTIGGPKELTAFLHNM a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c

2280 155 160 165 170 GDHVTRLDRWEPELNEAIPN AmpR GDHVTRLDRWEPELNEAIPN g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g

PstI 2340 175 180 185 190 DERDTTMPAAMATTLRKLLT AmpR DERDTTMPAAMATTLRKLLT g a c g a g c g t g a c a c c a c g a t g c c t g c a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t c t g c t c g c a c t g t g g t g c t a c g g a c g t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a

2400 195 200 205 210 GELLTLASRQQLIDWMEADK AmpR GELLTLASRQQLIDWMEADK g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t

2460 215 220 225 230 VAGPLLRSALPAGWFIADKS AmpR VAGPLLRSALPAGWFIADKS g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a

BsaI 2520 235 240 245 250 GAGERGSRGIIAALGPDGKP AmpR GAGERGSRGIIAALGPDGKP g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g

AhdI 2580 255 260 265 270 SRIVVIYTTGSQATMDERNR AmpR SRIVVIYTTGSQATMDERNR t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t

2640 275 280 285 QIAEIGASLIKHW* AmpR QIAEIGASLIKHW* c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g

2700 t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c

2760 a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c

2820 ori t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g

2880 ori t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c

2940 ori c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g

3000 ori c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g

AlwNI 3060 ori c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g

3120 ori g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a

3180 ori t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a

3240 ori g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g

3300 ori G G G A A A C G C C T G G T A T C T T pBR322ori-F g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a

3360 ori T pBR322ori-F t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t

3420 ori g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a

3480 t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a

3540 A G C G A G L4440 a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c

3600 T C A G T G A G C G A G L4440 t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g

PflMI 3660 lacIq promoter g g t a t t t c a c a c c g c a t a a a t t c c g a c a c c a t c g a a t g g t g c a a a a c c t t t c g c g g t a t g c c a t a a a g t g t g g c g t a t t t a a g g c t g t g g t a g c t t a c c a c g t t t t g g a a a g c g c c a t a c

3720 1 5 MKPVTL lacIq promoter lacI g c a t g a t a g c g c c c g g a a g a g a g t c a a t t c a g g g t g g t g a a t g t g a a a c c a g t a a c g t t a c g t a c t a t c g c g g g c c t t c t c t c a g t t a a g t c c c a c c a c t t a c a c t t t g g t c a t t g c a a t

BstAPI 3780 10 15 20 25 YDVAEYAGVSYQTVSRVVNQ lacI T G C T A C A G C G T C T C A T A C G G LacI-R t a c g a t g t c g c a g a g t a t g c c g g t g t c t c t t a t c a g a c c g t t t c c c g c g t g g t g a a c c a g a t g c t a c a g c g t c t c a t a c g g c c a c a g a g a a t a g t c t g g c a a a g g g c g c a c c a c t t g g t c

3840 30 35 40 45 ASHVSAKTREKVEAAMAELN lacI MAELN g c c a g c c a c g t t t c t g c g a a a a c g c g g g a a a a a g t g g a a g c g g c g a t g g c g g a g c t g a a t c g g t c g g t g c a a a g a c g c t t t t g c g c c c t t t t t c a c c t t c g c c g c t a c c g c c t c g a c t t a

3900 50 55 60 65 YIPNRVAQQLAGKQSLLIGV lacI YIPNRVAQQLAGKQSLLIGV t a c a t t c c c a a c c g c g t g g c a c a a c a a c t g g c g g g c a a a c a g t c g t t g c t g a t t g g c g t t a t g t a a g g g t t g g c g c a c c g t g t t g t t g a c c g c c c g t t t g t c a g c a a c g a c t a a c c g c a a

3960 70 75 80 85 ATSSLALHAPSQIVAAIKSR lacI ATSSLALHAPSQIVAAIKSR g c c a c c t c c a g t c t g g c c c t g c a c g c g c c g t c g c a a a t t g t c g c g g c g a t t a a a t c t c g c c g g t g g a g g t c a g a c c g g g a c g t g c g c g g c a g c g t t t a a c a g c g c c g c t a a t t t a g a g c g

4020 90 95 100 105 ADQLGASVVVSMVERSGVEA lacI ADQLGASVVVSMVERSGVEA g c c g a t c a a c t g g g t g c c a g c g t g g t g g t g t c g a t g g t a g a a c g a a g c g g c g t c g a a g c c c g g c t a g t t g a c c c a c g g t c g c a c c a c c a c a g c t a c c a t c t t g c t t c g c c g c a g c t t c g g

MluI 4080 110 115 120 125 CKAAVHNLLAQRVSGLIINY lacI CKAAVHNLLAQRVSGLIINY t g t a a a g c g g c g g t g c a c a a t c t t c t c g c g c a a c g c g t c a g t g g g c t g a t c a t t a a c t a t a c a t t t c g c c g c c a c g t g t t a g a a g a g c g c g t t g c g c a g t c a c c c g a c t a g t a a t t g a t a

4140 130 135 140 145 PLDDQDAIAVEAACTNVPAL lacI PLDDQDAIAVEAACTNVPAL c c g c t g g a t g a c c a g g a t g c c a t t g c t g t g g a a g c t g c c t g c a c t a a t g t t c c g g c g t t a g g c g a c c t a c t g g t c c t a c g g t a a c g a c a c c t t c g a c g g a c g t g a t t a c a a g g c c g c a a t

4200 150 155 160 165 FLDVSDQTPINSIIFSHEDG lacI FLDVSDQTPINSIIFSHEDG t t t c t t g a t g t c t c t g a c c a g a c a c c c a t c a a c a g t a t t a t t t t c t c c c a t g a a g a c g g t a a a g a a c t a c a g a g a c t g g t c t g t g g g t a g t t g t c a t a a t a a a a g a g g g t a c t t c t g c c a

BstEII 4260 170 175 180 185 TRLGVEHLVALGHQQIALLA lacI TRLGVEHLVALGHQQIALLA a c g c g a c t g g g c g t g g a g c a t c t g g t c g c a t t g g g t c a c c a g c a a a t c g c g c t g t t a g c g t g c g c t g a c c c g c a c c t c g t a g a c c a g c g t a a c c c a g t g g t c g t t t a g c g c g a c a a t c g c

4320 190 195 200 205 GPLSSVSARLRLAGWHKYLT lacI GPLSSVSARLRLAGWHKYLT g g c c c a t t a a g t t c t g t c t c g g c g c g t c t g c g t c t g g c t g g c t g g c a t a a a t a t c t c a c t c c g g g t a a t t c a a g a c a g a g c c g c g c a g a c g c a g a c c g a c c g a c c g t a t t t a t a g a g t g a

4380 210 215 220 225 RNQIQPIAEREGDWSAMSGF lacI RNQIQPIAEREGDWSAMSGF c g c a a t c a a a t t c a g c c g a t a g c g g a a c g g g a a g g c g a c t g g a g t g c c a t g t c c g g t t t t g c g t t a g t t t a a g t c g g c t a t c g c c t t g c c c t t c c g c t g a c c t c a c g g t a c a g g c c a a a a

4440 230 235 240 245 QQTMQMLNEGIVPTAMLVAN lacI QQTMQMLNEGIVPTAMLVAN c a a c a a a c c a t g c a a a t g c t g a a t g a g g g c a t c g t t c c c a c t g c g a t g c t g g t t g c c a a c g t t g t t t g g t a c g t t t a c g a c t t a c t c c c g t a g c a a g g g t g a c g c t a c g a c c a a c g g t t g

BssHII 4500 250 255 260 265 DQMALGAMRAITESGLRVGA lacI DQMALGAMRAITESGLRVGA g a t c a g a t g g c g c t g g g c g c a a t g c g c g c c a t t a c c g a g t c c g g g c t g c g c g t t g g t g c g c t a g t c t a c c g c g a c c c g c g t t a c g c g c g g t a a t g g c t c a g g c c c g a c g c g c a a c c a c g c

EcoRV 4560 270 275 280 285 DISVVGYDDTEDSSCYIPPS lacI DISVVGYDDTEDSSCYIPPS g a t a t c t c g g t a g t g g g a t a c g a c g a t a c c g a a g a c a g c t c a t g t t a t a t c c c g c c g t c a c t a t a g a g c c a t c a c c c t a t g c t g c t a t g g c t t c t g t c g a g t a c a a t a t a g g g c g g c a g t

4620 290 295 300 305 TTIKQDFRLLGQTSVDRLLQ lacI TTIKQDFRLLGQTSVDRLLQ a c c a c c a t c a a a c a g g a t t t t c g c c t g c t g g g g c a a a c c a g c g t g g a c c g c t t g c t g c a a t g g t g g t a g t t t g t c c t a a a a g c g g a c g a c c c c g t t t g g t c g c a c c t g g c g a a c g a c g t t

4680 310 315 320 325 LSQGQAVKGNQLLPVSLVKR lacI LSQGQAVKGNQLLPVSLVKR c t c t c t c a g g g c c a g g c g g t g a a g g g c a a t c a g c t g t t g c c c g t c t c a c t g g t g a a a a g a g a g a g a g t c c c g g t c c g c c a c t t c c c g t t a g t c g a c a a c g g g c a g a g t g a c c a c t t t t c t

KasINarI*SfoIPluTI 4740 330 335 340 345 KTTLAPNTQTASPRALADSL lacI KTTLAPNTQTASPRALADSL a a a a c c a c c c t g g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a t t t t g g t g g g a c c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t

4800 350 355 360 MQLARQVSRLESGQ* lacI CAP binding site MQLARQVSRLESGQ* a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t

4860 -35 -10 CAP binding site lac promoter t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a

4920 -10 1 MTMI lac promoter lac operator lacZ α M13 rev C A G G A A A C A G C T A T G A C M13 Reverse MTMI g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t

4980 5 10 15 20 TDSLAVVLQRRDWENPGVTQ lacZ α M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward TDSLAVVLQRRDWENPGVTQ c g g a t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c g c c t a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g

5040 25 30 35 40 LNRLAAHPPFASWRNSEEAR lacZ α LNRLAAHPPFASWRNSEEAR t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t

5100 45 50 55 60 TDRPSQQLRSLNGEWRFAWF lacZ α TDRPSQQLRSLNGEWRFAWF c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g c g c t t t g c c t g g t t t c g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c g c g a a a c g g a c c a a a g

Bsu36I 5160 65 70 75 80 PAPEAVPESWLECDLPEADT lacZ α PAPEAVPESWLECDLPEADT c g g c a c c a g a a g c g g t g c c g g a a a g c t g g c t g g a g t g c g a t c t t c c t g a g g c c g a t a c t g g c c g t g g t c t t c g c c a c g g c c t t t c g a c c g a c c t c a c g c t a g a a g g a c t c c g g c t a t g a c

5220 85 90 95 100 VVVPSNWQMHGYDAPIYTNV lacZ α VVVPSNWQMHGYDAPIYTNV t c g t c g t c c c c t c a a a c t g g c a g a t g c a c g g t t a c g a t g c g c c c a t c t a c a c c a a c g t a a a g c a g c a g g g g a g t t t g a c c g t c t a c g t g c c a a t g c t a c g c g g g t a g a t g t g g t t g c a t t

BtgI 5280 105 110 115 120 TYPITVNPPFVPTENPTGCY lacZ α TYPITVNPPFVPTENPTGCY c c t a t c c c a t t a c g g t c a a t c c g c c g t t t g t t c c c a c g g a g a a t c c g a c g g g t t g t t a c t g g a t a g g g t a a t g c c a g t t a g g c g g c a a a c a a g g g t g c c t c t t a g g c t g c c c a a c a a t g a

5340 125 130 135 140 SLTFNVDESWLQEGQTRIIF lacZ α SLTFNVDESWLQEGQTRIIF c g c t c a c a t t t a a t g t t g a t g a a a g c t g g c t a c a g g a a g g c c a g a c g c g a a t t a t t t t t g g c g a g t g t a a a t t a c a a c t a c t t t c g a c c g a t g t c c t t c c g g t c t g c g c t t a a t a a a a a c

5354 145 DGVGI lacZ α DGVGI a t g g c g t t g g a a t t t a c c g c a a c c t t a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.