Addgene pGEX SHP-1(C)-SH2 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pGEX SHP-1(C)-SH2


Map View

M13/pUC Forward (4924 .. 4946) M13 Forward (4915 .. 4932) M13 Reverse (4883 .. 4899) lac operator EcoRV (4490) MluI (4040) LacI-R (3709 .. 3728) BstAPI (3723) PflMI (3622) L4440 (3522 .. 3539) pBR322ori-F (3269 .. 3288) AlwNI (3015) lac operator EcoNI (268) BstBI (655) SwaI (685) pGEX 5' (869 .. 891) PasI (941) BamHI (945) AscI (953) SpeI (966) Acc65I (979) KpnI - NcoI (983) BbvCI (1065) CsiI - SexAI * (1216) StuI (1254) BseRI (1263) PacI (1306) EcoRI (1311) TspMI - XmaI (1316) SmaI (1318) SalI (1321) PaeR7I - PspXI - XhoI (1326) EagI - NotI (1332) PfoI (1408) pGEX 3' (1391 .. 1413) PflFI - Tth111I (1511) BsaAI (1518) pBRforEco (1558 .. 1576) ZraI (1615) AatII (1617) Amp-R (1967 .. 1986) AhdI (2536) pGEX SHP-1(C)-SH2 5341 bp

Sequence View

60 150 155 160 165 TLSTARCTNASGVRQPSEAV lacZ α TLSTARCTNASGVRQPSEAV a c g t t a t c g a c t g c a c g g t g c a c c a a t g c t t c t g g c g t c a g g c a g c c a t c g g a a g c t g t g t g c a a t a g c t g a c g t g c c a c g t g g t t a c g a a g a c c g c a g t c c g t c g g t a g c c t t c g a c a c

120 170 175 VWLCRS* lacZ α VWLCRS* g t a t g g c t g t g c a g g t c g t a a a t c a c t g c a t a a t t c g t g t c g c t c a a g g c g c a c t c c c g t c a t a c c g a c a c g t c c a g c a t t t a g t g a c g t a t t a a g c a c a g c g a g t t c c g c g t g a g g g c a

180 t c t g g a t a a t g t t t t t t g c g c c g a c a t c a t a a c g g t t c t g g c a a a t a t t c t g a a a t g a g c a g a c c t a t t a c a a a a a a c g c g g c t g t a g t a t t g c c a a g a c c g t t t a t a a g a c t t t a c t c g

240 -35 -10 tac promoter lac operator t g t t g a c a a t t a a t c a t c g g c t c g t a t a a t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a a c a a c t g t t a a t t a g t a g c c g a g c a t a t t a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t

EcoNI 300 1 5 10 MSPILGYWKIKGLV GST MSPILGYWKIKGLV c a c a g g a a a c a g t a t t c a t g t c c c c t a t a c t a g g t t a t t g g a a a a t t a a g g g c c t t g t g c g t g t c c t t t g t c a t a a g t a c a g g g g a t a t g a t c c a a t a a c c t t t t a a t t c c c g g a a c a c g

360 15 20 25 30 QPTRLLLEYLEEKYEEHLYE GST QPTRLLLEYLEEKYEEHLYE a a c c c a c t c g a c t t c t t t t g g a a t a t c t t g a a g a a a a a t a t g a a g a g c a t t t g t a t g a g c t t g g g t g a g c t g a a g a a a a c c t t a t a g a a c t t c t t t t t a t a c t t c t c g t a a a c a t a c t c g

420 35 40 45 50 RDEGDKWRNKKFELGLEFPN GST RDEGDKWRNKKFELGLEFPN g c g a t g a a g g t g a t a a a t g g c g a a a c a a a a a g t t t g a a t t g g g t t t g g a g t t t c c c a a t c c g c t a c t t c c a c t a t t t a c c g c t t t g t t t t t c a a a c t t a a c c c a a a c c t c a a a g g g t t a g

480 55 60 65 70 LPYYIDGDVKLTQSMAIIRY GST LPYYIDGDVKLTQSMAIIRY t t c c t t a t t a t a t t g a t g g t g a t g t t a a a t t a a c a c a g t c t a t g g c c a t c a t a c g t t a t a a a g g a a t a a t a t a a c t a c c a c t a c a a t t t a a t t g t g t c a g a t a c c g g t a g t a t g c a a t a t

540 75 80 85 90 IADKHNMLGGCPKERAEISM GST IADKHNMLGGCPKERAEISM t a g c t g a c a a g c a c a a c a t g t t g g g t g g t t g t c c a a a a g a g c g t g c a g a g a t t t c a a t g c a t c g a c t g t t c g t g t t g t a c a a c c c a c c a a c a g g t t t t c t c g c a c g t c t c t a a a g t t a c g

600 95 100 105 110 LEGAVLDIRYGVSRIAYSKD GST LEGAVLDIRYGVSRIAYSKD t t g a a g g a g c g g t t t t g g a t a t t a g a t a c g g t g t t t c g a g a a t t g c a t a t a g t a a a g a c t a a c t t c c t c g c c a a a a c c t a t a a t c t a t g c c a c a a a g c t c t t a a c g t a t a t c a t t t c t g a

BstBI 660 115 120 125 130 FETLKVDFLSKLPEMLKMFE GST FETLKVDFLSKLPEMLKMFE t t g a a a c t c t c a a a g t t g a t t t t c t t a g c a a g c t a c c t g a a a t g c t g a a a a t g t t c g a a g a a c t t t g a g a g t t t c a a c t a a a a g a a t c g t t c g a t g g a c t t t a c g a c t t t t a c a a g c t t c

SwaI 720 135 140 145 150 DRLCHKTYLNGDHVTHPDFM GST DRLCHKTYLNGDHVTHPDFM *TVWGSKMN a t c g t t t a t g t c a t a a a a c a t a t t t a a a t g g t g a t c a t g t a a c c c a t c c t g a c t t c a t g t t a g c a a a t a c a g t a t t t t g t a t a a a t t t a c c a c t a g t a c a t t g g g t a g g a c t g a a g t a c a

780 155 160 165 170 LYDALDVVLYMDPMCLDAFP GST LYDALDVVLYMDPMCLDAFP YSARSTTKYMSGIHRSANGF t g t a t g a c g c t c t t g a t g t t g t t t t a t a c a t g g a c c c a a t g t g c c t g g a t g c g t t c c c a a a c a t a c t g c g a g a a c t a c a a c a a a a t a t g t a c c t g g g t t a c a c g g a c c t a c g c a a g g g t t

840 175 180 185 190 KLVCFKKRIEAIPQIDKYLK GST KLVCFKKRIEAIPQIDKYLK NTQKLFRISAIGCISLYKFD a a t t a g t t t g t t t t a a a a a a c g t a t t g a a g c t a t c c c a c a a a t t g a t a a g t a c t t g a a a t t t a a t c a a a c a a a a t t t t t t g c a t a a c t t c g a t a g g g t g t t t a a c t a t t c a t g a a c t t t a

900 195 200 205 210 SSKYIAWPLQGWQATFGGGD GST G G G C T G G C A A G C C A C G T T T G G T G pGEX 5' SSKYIAWPLQGWQATFGGGD LLYIAHGKCPQCAVNPPPSW c c a g c a a g t a t a t a g c a t g g c c t t t g c a g g g c t g g c a a g c c a c g t t t g g t g g t g g c g a c c g g t c g t t c a t a t a t c g t a c c g g a a a c g t c c c g a c c g t t c g g t g c a a a c c a c c a c c g c t g g

AscIBamHIPasI 960 215 HPPK SD 1 5 LEVLFQGP (in frame with HRV 3C site) LGSGRA GST HRV 3C site HPPKSDLEVLFQGPLGSGRA GGFDSRSTRNWPGRPDPRAM a t c c t c c a a a a t c g g a t c t g g a a g t t c t g t t c c a g g g g c c c c t g g g a t c c g g g c g c g c c a t a g g a g g t t t t a g c c t a g a c c t t c a a g a c a a g g t c c c c g g g g a c c c t a g g c c c g c g c g g t

Acc65IKpnINcoISpeI 1020 (in frame with HRV 3C site) MPTSERWYHGHMSGGQAETL MPTSERWYHGHMSGGQAETL GVLSLHYWPWMDPPCASVSS t g c c c a c t a g t g a g a g g t g g t a c c a t g g c c a c a t g t c t g g c g g g c a g g c a g a g a c g c t g c a c g g g t g a t c a c t c t c c a c c a t g g t a c c g g t g t a c a g a c c g c c c g t c c g t c t c t g c g a c g

BbvCI 1080 (in frame with HRV 3C site) LQAKGEPWTFLVRESLSQPG LQAKGEPWTFLVRESLSQPG CALPSGQVNRTRSLRLWGPS t g c a g g c c a a g g g c g a g c c c t g g a c g t t t c t t g t g c g t g a g a g c c t c a g c c a g c c t g g a g a c g t c c g g t t c c c g c t c g g g a c c t g c a a a g a a c a c g c a c t c t c g g a g t c g g t c g g a c c t c

1140 (in frame with HRV 3C site) DFVLSVLSDQPKAGPGSPLR DFVLSVLSDQPKAGPGSPLR KTSETSLSWGLAPGPEGSLT a c t t c g t g c t t t c t g t g c t c a g t g a c c a g c c c a a g g c t g g c c c a g g c t c c c c g c t c a g g g t g a a g c a c g a a a g a c a c g a g t c a c t g g t c g g g t t c c g a c c g g g t c c g a g g g g c g a g t c c c

1200 (in frame with HRV 3C site) VTHIKVMCEGGRYTVGGLET VTHIKVMCEGGRYTVGGLET VWMLTM t c a c c c a c a t c a a g g t c a t g t g c g a g g g t g g a c g c t a c a c a g t g g g t g g t t t g g a g a c c t a g t g g g t g t a g t t c c a g t a c a c g c t c c c a c c t g c g a t g t g t c a c c c a c c a a a c c t c t g g a

StuISexAI*CsiI 1260 (in frame with HRV 3C site) FDSLTDLVEHFKKTGIEEAS FDSLTDLVEHFKKTGIEEAS t c g a c a g c c t c a c g g a c c t g g t g g a g c a t t t c a a g a a g a c g g g g a t t g a g g a g g c c t c a g a g c t g t c g g a g t g c c t g g a c c a c c t c g t a a a g t t c t t c t g c c c c t a a c t c c t c c g g a g t c

PacIEcoRITspMIXmaISmaIBseRI 1320 (in frame with HRV 3C site) GAFVYLRQPYYATRLINEFP GAFVYLRQPYYATRLINEFP g c g c c t t t g t c t a c c t g c g g c a g c c g t a c t a t g c c a c g a g g t t a a t t a a c g a a t t c c c g g c g c g g a a a c a g a t g g a c g c c g t c g g c a t g a t a c g g t g c t c c a a t t a a t t g c t t a a g g g c c

EagINotIPaeR7IPspXIXhoISalI 1380 (in frame with HRV 3C site) GRLERPHRD* GRLERPHRD* g t c g a c t c g a g c g g c c g c a t c g t g a c t g a c t g a c g a t c t g c c t c g c g c g t t t c g g t g a t g c a g c t g a g c t c g c c g g c g t a g c a c t g a c t g a c t g c t a g a c g g a g c g c g c a a a g c c a c t a c

PfoI 1440 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g t a a g c g g t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c a t t c g c c

1500 a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g t c g g g g c g t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c a g c c c c g c

BsaAITth111IPflFI 1560 C G G pBRforEco c a g c c a t g a c c c a g t c a c g t a g c g a t a g c g g a g t g t a t a a t t c t t g a a g a c g a a a g g g c c g t c g g t a c t g g g t c a g t g c a t c g c t a t c g c c t c a c a t a t t a a g a a c t t c t g c t t t c c c g g

ZraIAatII 1620 A G C A C T A T G C G G A T A A pBRforEco t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c

1680 AmpR promoter g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a

1740 AmpR promoter c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t

1800 1 5 10 15 signal sequence MSIQHFRVALIPFFAAF AmpR promoter AmpR MSIQHFRVALIPFFAAF g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a

1860 20 signal sequence CLPVFA 25 30 35 HPETLVKVKDAEDQ AmpR CLPVFAHPETLVKVKDAEDQ g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a

1920 40 45 50 55 LGARVGYIELDLNSGKILES AmpR LGARVGYIELDLNSGKILES t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a

1980 60 65 70 75 FRPEERFPMMSTFKVLLCGA AmpR C G A T A C A C C G C G C C Amp-R FRPEERFPMMSTFKVLLCGA t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c

2040 80 85 90 95 VLSRVDAGQEQLGRRIHYSQ AmpR A T A A T A Amp-R VLSRVDAGQEQLGRRIHYSQ t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t

2100 100 105 110 115 NDLVEYSPVTEKHLTDGMTV AmpR NDLVEYSPVTEKHLTDGMTV a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t

2160 120 125 130 135 RELCSAAITMSDNTAANLLL AmpR RELCSAAITMSDNTAANLLL g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t

2220 140 145 150 155 TTIGGPKELTAFLHNMGDHV AmpR TTIGGPKELTAFLHNMGDHV c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t

2280 160 165 170 175 TRLDRWEPELNEAIPNDERD AmpR TRLDRWEPELNEAIPNDERD c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t

2340 180 185 190 195 TTMPAAMATTLRKLLTGELL AmpR TTMPAAMATTLRKLLTGELL c c a c g a t g c c t g c a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a g g t g c t a c g g a c g t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t

2400 200 205 210 215 TLASRQQLIDWMEADKVAGP AmpR TLASRQQLIDWMEADKVAGP c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g

2460 220 225 230 235 LLRSALPAGWFIADKSGAGE AmpR LLRSALPAGWFIADKSGAGE t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g

2520 240 245 250 255 RGSRGIIAALGPDGKPSRIV AmpR RGSRGIIAALGPDGKPSRIV g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c

AhdI 2580 260 265 270 275 VIYTTGSQATMDERNRQIAE AmpR VIYTTGSQATMDERNRQIAE t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t

2640 280 285 IGASLIKHW* AmpR IGASLIKHW* t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a

2700 a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t

2760 a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c

2820 ori a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t

2880 ori c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a

2940 ori t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g

3000 ori c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t

AlwNI 3060 ori t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t

3120 ori g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g

3180 ori c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t

3240 ori g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t

3300 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c

3360 ori g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g

3420 ori t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c

3480 c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c

3540 A G C G A G T C A G T G A G C G A G L4440 a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c

3600 a a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g

PflMI 3660 lacIq promoter g c a t a a a t t c c g a c a c c a t c g a a t g g t g c a a a a c c t t t c g c g g t a t g g c a t g a t a g c g c c c g t a t t t a a g g c t g t g g t a g c t t a c c a c g t t t t g g a a a g c g c c a t a c c g t a c t a t c g c g g

3720 1 5 10 MKPVTLYDVA lacIq promoter lacI T G C T A C A G C G T C LacI-R c g g a a g a g a g t c a a t t c a g g g t g g t g a a t g t g a a a c c a g t a a c g t t a t a c g a t g t c g c a g g c c t t c t c t c a g t t a a g t c c c a c c a c t t a c a c t t t g g t c a t t g c a a t a t g c t a c a g c g t c

BstAPI 3780 15 20 25 30 EYAGVSYQTVSRVVNQASHV lacI T C A T A C G G LacI-R a g t a t g c c g g t g t c t c t t a t c a g a c c g t t t c c c g c g t g g t g a a c c a g g c c a g c c a c g t t t t c a t a c g g c c a c a g a g a a t a g t c t g g c a a a g g g c g c a c c a c t t g g t c c g g t c g g t g c a a a

3840 35 40 45 50 SAKTREKVEAAMAELNYIPN lacI MAELNYIPN c t g c g a a a a c g c g g g a a a a a g t g g a a g c g g c g a t g g c g g a g c t g a a t t a c a t t c c c a a c c g a c g c t t t t g c g c c c t t t t t c a c c t t c g c c g c t a c c g c c t c g a c t t a a t g t a a g g g t t g g

3900 55 60 65 70 RVAQQLAGKQSLLIGVATSS lacI RVAQQLAGKQSLLIGVATSS g c g t g g c a c a a c a a c t g g c g g g c a a a c a g t c g t t g c t g a t t g g c g t t g c c a c c t c c a g t c c g c a c c g t g t t g t t g a c c g c c c g t t t g t c a g c a a c g a c t a a c c g c a a c g g t g g a g g t c a g

3960 75 80 85 90 LALHAPSQIVAAIKSRADQL lacI LALHAPSQIVAAIKSRADQL t g g c c c t g c a c g c g c c g t c g c a a a t t g t c g c g g c g a t t a a a t c t c g c g c c g a t c a a c t g g a c c g g g a c g t g c g c g g c a g c g t t t a a c a g c g c c g c t a a t t t a g a g c g c g g c t a g t t g a c c

4020 95 100 105 110 GASVVVSMVERSGVEACKAA lacI GASVVVSMVERSGVEACKAA g t g c c a g c g t g g t g g t g t c g a t g g t a g a a c g a a g c g g c g t c g a a g c c t g t a a a g c g g c g g c a c g g t c g c a c c a c c a c a g c t a c c a t c t t g c t t c g c c g c a g c t t c g g a c a t t t c g c c g c c

MluI 4080 115 120 125 130 VHNLLAQRVSGLIINYPLDD lacI VHNLLAQRVSGLIINYPLDD t g c a c a a t c t t c t c g c g c a a c g c g t c a g t g g g c t g a t c a t t a a c t a t c c g c t g g a t g a c c a c g t g t t a g a a g a g c g c g t t g c g c a g t c a c c c g a c t a g t a a t t g a t a g g c g a c c t a c t g g

4140 135 140 145 150 QDAIAVEAACTNVPALFLDV lacI QDAIAVEAACTNVPALFLDV a g g a t g c c a t t g c t g t g g a a g c t g c c t g c a c t a a t g t t c c g g c g t t a t t t c t t g a t g t c t t c c t a c g g t a a c g a c a c c t t c g a c g g a c g t g a t t a c a a g g c c g c a a t a a a g a a c t a c a g a

4200 155 160 165 170 SDQTPINSIIFSHEDGTRLG lacI SDQTPINSIIFSHEDGTRLG c t g a c c a g a c a c c c a t c a a c a g t a t t a t t t t c t c c c a t g a a g a c g g t a c g c g a c t g g g c g g a c t g g t c t g t g g g t a g t t g t c a t a a t a a a a g a g g g t a c t t c t g c c a t g c g c t g a c c c g c

4260 175 180 185 190 VEHLVALGHQQIALLAGPLS lacI VEHLVALGHQQIALLAGPLS t g g a g c a t c t g g t c g c a t t g g g t c a c c a g c a a a t c g c g c t g t t a g c g g g c c c a t t a a g t t a c c t c g t a g a c c a g c g t a a c c c a g t g g t c g t t t a g c g c g a c a a t c g c c c g g g t a a t t c a a

4320 195 200 205 210 SVSARLRLAGWHKYLTRNQI lacI SVSARLRLAGWHKYLTRNQI c t g t c t c g g c g c g t c t g c g t c t g g c t g g c t g g c a t a a a t a t c t c a c t c g c a a t c a a a t t c g a c a g a g c c g c g c a g a c g c a g a c c g a c c g a c c g t a t t t a t a g a g t g a g c g t t a g t t t a a g

4380 215 220 225 230 QPIAEREGDWSAMSGFQQTM lacI QPIAEREGDWSAMSGFQQTM a g c c g a t a g c g g a a c g g g a a g g c g a c t g g a g t g c c a t g t c c g g t t t t c a a c a a a c c a t g c t c g g c t a t c g c c t t g c c c t t c c g c t g a c c t c a c g g t a c a g g c c a a a a g t t g t t t g g t a c g

4440 235 240 245 250 QMLNEGIVPTAMLVANDQMA lacI QMLNEGIVPTAMLVANDQMA a a a t g c t g a a t g a g g g c a t c g t t c c c a c t g c g a t g c t g g t t g c c a a c g a t c a g a t g g c g c t t t a c g a c t t a c t c c c g t a g c a a g g g t g a c g c t a c g a c c a a c g g t t g c t a g t c t a c c g c g

EcoRV 4500 255 260 265 270 LGAMRAITESGLRVGADISV lacI LGAMRAITESGLRVGADISV t g g g c g c a a t g c g c g c c a t t a c c g a g t c c g g g c t g c g c g t t g g t g c g g a t a t c t c g g t a g a c c c g c g t t a c g c g c g g t a a t g g c t c a g g c c c g a c g c g c a a c c a c g c c t a t a g a g c c a t c

4560 275 280 285 290 VGYDDTEDSSCYIPPSTTIK lacI VGYDDTEDSSCYIPPSTTIK t g g g a t a c g a c g a t a c c g a a g a c a g c t c a t g t t a t a t c c c g c c g t c a a c c a c c a t c a a a c a c c c t a t g c t g c t a t g g c t t c t g t c g a g t a c a a t a t a g g g c g g c a g t t g g t g g t a g t t t g

4620 295 300 305 310 QDFRLLGQTSVDRLLQLSQG lacI QDFRLLGQTSVDRLLQLSQG a g g a t t t t c g c c t g c t g g g g c a a a c c a g c g t g g a c c g c t t g c t g c a a c t c t c t c a g g g c c t c c t a a a a g c g g a c g a c c c c g t t t g g t c g c a c c t g g c g a a c g a c g t t g a g a g a g t c c c g g

4680 315 320 325 330 QAVKGNQLLPVSLVKRKTTL lacI QAVKGNQLLPVSLVKRKTTL a g g c g g t g a a g g g c a a t c a g c t g t t g c c c g t c t c a c t g g t g a a a a g a a a a a c c a c c c t g g t c c g c c a c t t c c c g t t a g t c g a c a a c g g g c a g a g t g a c c a c t t t t c t t t t t g g t g g g a c c

4740 335 340 345 350 APNTQTASPRALADSLMQLA lacI APNTQTASPRALADSLMQLA c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g

4800 355 360 RQVSRLESGQ* lacI CAP binding site RQVSRLESGQ* g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g

4860 -35 -10 CAP binding site lac promoter lac operator a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a

4920 1 5 MTMITDSLA lac operator lacZ α M13 rev M13 fwd C A G G A A A C A G C T A T G A C M13 Reverse T G A C C G M13 Forward MTMITDSLA g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g g a t t c a c t g g c c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c c t a a g t g a c c g

4980 10 15 20 25 VVLQRRDWENPGVTQLNRLA lacZ α M13 fwd G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward VVLQRRDWENPGVTQLNRLA c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g

5040 30 35 40 45 AHPPFASWRNSEEARTDRPS lacZ α AHPPFASWRNSEEARTDRPS a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g

5100 50 55 60 65 QQLRSLNGEWRFAWFPAPEA lacZ α QQLRSLNGEWRFAWFPAPEA c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g c g c t t t g c c t g g t t t c c g g c a c c a g a a g c g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c g c g a a a c g g a c c a a a g g c c g t g g t c t t c g

5160 70 75 80 85 VPESWLECDLPEADTVVVPS lacZ α VPESWLECDLPEADTVVVPS g g t g c c g g a a a g c t g g c t g g a g t g c g a t c t t c c t g a g g c c g a t a c t g t c g t c g t c c c c t c c c a c g g c c t t t c g a c c g a c c t c a c g c t a g a a g g a c t c c g g c t a t g a c a g c a g c a g g g g a g

5220 90 95 100 105 NWQMHGYDAPIYTNVTYPIT lacZ α NWQMHGYDAPIYTNVTYPIT a a a c t g g c a g a t g c a c g g t t a c g a t g c g c c c a t c t a c a c c a a c g t a a c c t a t c c c a t t a c t t t g a c c g t c t a c g t g c c a a t g c t a c g c g g g t a g a t g t g g t t g c a t t g g a t a g g g t a a t g

5280 110 115 120 125 VNPPFVPTENPTGCYSLTFN lacZ α VNPPFVPTENPTGCYSLTFN g g t c a a t c c g c c g t t t g t t c c c a c g g a g a a t c c g a c g g g t t g t t a c t c g c t c a c a t t t a a c c a g t t a g g c g g c a a a c a a g g g t g c c t c t t a g g c t g c c c a a c a a t g a g c g a g t g t a a a t t

5340 130 135 140 145 VDESWLQEGQTRIIFDGVGI lacZ α VDESWLQEGQTRIIFDGVGI t g t t g a t g a a a g c t g g c t a c a g g a a g g c c a g a c g c g a a t t a t t t t t g a t g g c g t t g g a a t a c a a c t a c t t t c g a c c g a t g t c c t t c c g g t c t g c g c t t a a t a a a a a c t a c c g c a a c c t t a

5341 lacZ α t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.