Addgene N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A> HP1aCS-Frbx2-V5-PGK-Blast Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A> HP1aCS-Frbx2-V5-PGK-Blast Sequencing Result

Map View

800 600 400 200 End (886) MflI * - BstYI (856) AgeI - BsrFI - BsaWI (841) BsiWI (818) HincII (810) AccI (809) SalI (808) BbsI (771) BmrI (749) BpmI (701) SmaI (604) XmaI - TspMI (602) BsiHKAI (560) ApaLI (556) BsgI (511) Esp3I - BsmBI (509) PaqCI (483) SmlI - XhoI - PspXI - PaeR7I (481) BstBI (476) SacII (472) MspA1I (471) ApaI - BanII (469) PspOMI - EcoO109I (465) TatI - BsrGI (443) EcoRI (424) BclI * (342) XcmI (273) BsaI (256) NotI EagI (231) SphI (174) AcuI (165) PvuI - AsiSI (90) NdeI (63) MmeI (23) StyI - NcoI (11) Start (0) Zif268 Oct-1 Kozak sequence c-myc NLS ZFHD1 attB2 DmrB HA FKBP N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A> HP1aCS-Frbx2-V5-PGK-Blast 886 bp

Sequence View

MmeINcoIStyIStart (0) 5ʹ 3ʹ 60 1 5 PAAKRVKLD SR 335 Zif268 ER Kozak sequence c-myc NLS ZFHD1 GRATMDYPAAKRVKLDSRER g g c c g c g c c a c c a t g g a c t a t c c t g c t g c c a a g a g g g t c a a g t t g g a c t c t a g a g a a c g c c c g g c g c g g t g g t a c c t g a t a g g a c g a c g g t t c t c c c a g t t c a a c c t g a g a t c t c t t g c g

AsiSIPvuINdeI 120 340 345 350 355 Zif268 PYACPVESCDRRFSRSDELT ZFHD1 PYACPVESCDRRFSRSDELT c c a t a t g c t t g c c c t g t c g a g t c c t g c g a t c g c c g c t t t t c t c g c t c g g a t g a g c t t a c c g g t a t a c g a a c g g g a c a g c t c a g g a c g c t a g c g g c g a a a a g a g c g a g c c t a c t c g a a t g g

SphIAcuI 180 360 365 370 375 Zif268 RHIRIHTGQKPFQCRICMRN ZFHD1 RHIRIHTGQKPFQCRICMRN c g c c a t a t c c g c a t c c a c a c a g g c c a g a a g c c c t t c c a g t g t c g a a t c t g c a t g c g t a a c g c g g t a t a g g c g t a g g t g t g t c c g g t c t t c g g g a a g g t c a c a g c t t a g a c g t a c g c a t t g

EagINotI 240 380 385 390 Zif268 FSRSDHLTTHIRTHTG GG 390 Oct-1 RR ZFHD1 FSRSDHLTTHIRTHTGGGRR t t c a g t c g t a g t g a c c a c c t t a c c a c c c a c a t c c g c a c c c a c a c a g g c g g c g g c c g c a g g a a g t c a g c a t c a c t g g t g g a a t g g t g g g t g t a g g c g t g g g t g t g t c c g c c g c c g g c g t c c

XcmIBsaI 300 395 400 405 410 Oct-1 RKKRTSIETNIRVALEKSFL ZFHD1 RKKRTSIETNIRVALEKSFL a g g a a g a a a c g c a c c a g c a t a g a g a c c a a c a t c c g t g t g g c c t t a g a g a a g a g t t t c t t g t c c t t c t t t g c g t g g t c g t a t c t c t g g t t g t a g g c a c a c c g g a a t c t c t t c t c a a a g a a c

BclI* 360 415 420 425 430 Oct-1 ENQKPTSEEITMIADQLNME ZFHD1 ENQKPTSEEITMIADQLNME g a g a a t c a a a a g c c t a c c t c g g a a g a g a t c a c t a t g a t t g c t g a t c a g c t c a a t a t g g a a c t c t t a g t t t t c g g a t g g a g c c t t c t c t a g t g a t a c t a a c g a c t a g t c g a g t t a t a c c t t

420 435 440 445 450 Oct-1 KEVIRVWFCNRRQKEKRIN K ZFHD1 KEVIRVWFCNRRQKEKRINK a a a g a g g t g a t t c g t g t t t g g t t c t g t a a c c g c c g c c a g a a a g a a a a a a g a a t c a a c a a g t t t c t c c a c t a a g c a c a a a c c a a g a c a t t g g c g g c g g t c t t t c t t t t t t c t t a g t t g t t c

EcoO109IPspOMIBanIIApaIMspA1ISacIIBstBIBsrGITatIEcoRI 480 (in frame with ZFHD1) GEFDPAFLYKVVDLEGPRFE attB2 GEFDPAFLYKVVDLEGPRFE g g c g a a t t c g a c c c a g c t t t c t t g t a c a a a g t g g t t g a t c t a g a g g g c c c g c g g t t c g a a c c g c t t a a g c t g g g t c g a a a g a a c a t g t t t c a c c a a c t a g a t c t c c c g g g c g c c a a g c t t

BsmBIEsp3IBsgIPaeR7IPspXIXhoISmlIPaqCI 540 LE 1 5 10 15 GVQVETISPGDGRTFPKR DmrB 1 5 10 15 GVQVETISPGDGRTFPKR FKBP LEGVQVETISPGDGRTFPKR c t c g a g g g c g t g c a g g t g g a g a c t a t c t c c c c a g g a g a c g g g c g c a c c t t c c c c a a g c g c g a g c t c c c g c a c g t c c a c c t c t g a t a g a g g g g t c c t c t g c c c g c g t g g a a g g g g t t c g c g

ApaLIBsiHKAI 600 20 25 30 35 GQTCVVHYTGMLEDGKKFDS FKBP 20 25 30 35 GQTCVVHYTGMLEDGKKFDS DmrB GQTCVVHYTGMLEDGKKFDS g g c c a g a c c t g c g t g g t g c a c t a c a c c g g g a t g c t t g a a g a t g g a a a g a a a t t t g a t t c c c c g g t c t g g a c g c a c c a c g t g a t g t g g c c c t a c g a a c t t c t a c c t t t c t t t a a a c t a a g g

TspMIXmaISmaI 660 40 45 50 55 SRDRNKPFKFMLGKQEVIRG FKBP 40 45 50 55 SRDRNKPFKFMLGKQEVIRG DmrB SRDRNKPFKFMLGKQEVIRG t c c c g g g a c a g a a a c a a g c c c t t t a a g t t t a t g c t a g g c a a g c a g g a g g t g a t c c g a g g c a g g g c c c t g t c t t t g t t c g g g a a a t t c a a a t a c g a t c c g t t c g t c c t c c a c t a g g c t c c g

BpmI 720 60 65 70 75 WEEGVAQMSVGQRAKLTISP FKBP 60 65 70 75 WEEGVAQMSVGQRAKLTISP DmrB WEEGVAQMSVGQRAKLTISP t g g g a a g a a g g g g t t g c c c a g a t g a g t g t g g g t c a g a g a g c c a a a c t g a c t a t a t c t c c a a c c c t t c t t c c c c a a c g g g t c t a c t c a c a c c c a g t c t c t c g g t t t g a c t g a t a t a g a g g t

BbsIBmrI 780 80 85 90 95 DYAYGATGHPGIIPPHATLV FKBP 80 85 90 95 DYAYGATGHPGIIPPHATLV DmrB DYAYGATGHPGIIPPHATLV g a t t a t g c c t a t g g t g c c a c t g g g c a c c c a g g c a t c a t c c c a c c a c a t g c c a c t c t c g t c c t a a t a c g g a t a c c a c g g t g a c c c g t g g g t c c g t a g t a g g g t g g t g t a c g g t g a g a g c a g

BsiWISalIAccIHincII 840 100 105 FDVELLKLE VD 1 5 YPYDVPDYA DmrB HA 100 105 FDVELLKLE (in frame with FKBP) VDYPYDVPDYA FKBP FDVELLKLEVDYPYDVPDYA t t c g a t g t g g a g c t t c t a a a a c t g g a a g t c g a c t a t c c g t a c g a c g t a c c a g a c t a c g c a a a g c t a c a c c t c g a a g a t t t t g a c c t t c a g c t g a t a g g c a t g c t g c a t g g t c t g a t g c g t

End (886) BstYIMflI*BsaWIBsrFIAgeI 3ʹ 5ʹ 886 (in frame with FKBP) TGEGRGSLLTCGDVE (in frame with HA) TGEGRGSLLTCGDVE TGEGRGSLLTCGDVE a c c g g t g a a g g t c g t g g a t c t c t t c t c a c t t g t g g c g a t g t t g a a a t g g c c a c t t c c a g c a c c t a g a g a a g a g t g a a c a c c g c t a c a a c t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.