Addgene pCU-MET3 - Sequence Analyzer Skip to main content
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Sequence Analyzer: pCU-MET3


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PfoI (6256) pRS-marker (6138 .. 6157) SbfI (5919) XcmI (5770) NcoI (5688) BstBI (5630) BbsI (5619) BsmI (5537) ApaI * (5523) PspOMI * (5519) StuI (5461) Bpu10I (5164) NsiI (5074) F1ori-F (4806 .. 4827) NaeI (4636) NgoMIV (4634) F1ori-R (4596 .. 4615) M13/pUC Forward (4361 .. 4383) M13 Forward (4352 .. 4369) M13 fwd T7 (4323 .. 4342) KpnI (4314) Acc65I (4310) SnaBI (4066) pBluescriptKS (3894 .. 3910) PaeR7I - XhoI (3906) SalI (3900) EcoRI (3873) SmaI (3863) TspMI - XmaI (3861) SpeI (3849) XbaI (3843) pBluescriptSK (3843 .. 3859) BlpI (3682) SphI (3592) MfeI (3583) pGEX 3' (6257 .. 6279) pBRforEco (10 .. 28) SwaI (540) Amp-R (956 .. 975) BsaHI (985) NmeAIII (1378) AhdI (1525) pBR322ori-F (2258 .. 2277) L4440 (2511 .. 2528) CAP binding site M13/pUC Reverse (2724 .. 2746) lac operator M13 Reverse (2743 .. 2759) M13 rev T3 (2778 .. 2798) Eco53kI (2813) SacI (2815) KflI (3032) pCU-MET3 6307 bp

Sequence View

5ʹ 3ʹ 60 C G G A G C A C T A T G C G G A T A A pBRforEco g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a

120 c t t a g c a g g t t a g a c t a a c a t g c a a a a a g t t t a t a t t c a t t t t t g t a g t t a t t c t t g t t t g a a t c g t c c a a t c t g a t t g t a c g t t t t t c a a a t a t a a g t a a a a a c a t c a a t a a g a a c a a a

180 t c a t t a g g a t g a t t a c a c a c t g t g g a t g t g g a t t a a t c g a t c a t t g t t a a t t t a g t g t a g a g t a a t c c t a c t a a t g t g t g a c a c c t a c a c c t a a t t a g c t a g t a a c a a t t a a a t c a c a t c

240 c t a c t a c c a t t t c a a a c a a a a c g t a a a t t c t t g c a g t c g t a c t g g a t c t g t g a a t c t a t t g a t g a t g g t a a a g t t t g t t t t g c a t t t a a g a a c g t c a g c a t g a c c t a g a c a c t t a g a t a a

300 a g t a t a t a t g a a t t a a a g t a g c t t g t a c a t a t t a t t c t g t t g a a t c a t a t c g c a g a g c a t t c a t a t a t a c t t a a t t t c a t c g a a c a t g t a t a a t a a g a c a a c t t a g t a t a g c g t c t c g t a

360 t g g t t g a a a t c c a a a a t a t a a a a a t g t a a t a t c a c a a a a a a t a a t a c t a a t t c t a a c a t t a c c a a c t t t a g g t t t t a t a t t t t t a c a t t a t a g t g t t t t t t a t t a t g a t t a a g a t t g t a a

420 a a t g g g t c a g a t t t t t a g t g a a t a c t t a a a t t t a t a a t a t c g t c t a t t t a a g c t a g c a a a t t a c c c a g t c t a a a a a t c a c t t a t g a a t t t a a a t a t t a t a g c a g a t a a a t t c g a t c g t t t

480 t g g a a c a a c a t t t a a a g t a a g a a c a t c a t a t c t a c a t g a a a a t g t a t a t t t c a a t c t g a c a c c t t g t t g t a a a t t t c a t t c t t g t a g t a t a g a t g t a c t t t t a c a t a t a a a g t t a g a c t g

SwaI 540 t a a t a a c g c a g a g c a c a t c t t t c a g t g a t g t c t g t c a c a t g a t c a a a a a g a a t t g t a t t t a t t a t t g c g t c t c g t g t a g a a a g t c a c t a c a g a c a g t g t a c t a g t t t t t c t t a a c a t a a a

600 a a a t a a t t c a t a a t a a a a g c t t a a a a a a t t a c a a t a a t g a a a a t a a a g t a a a t a a t g a c a t t t a t t a a g t a t t a t t t t c g a a t t t t t t a a t g t t a t t a c t t t t a t t t c a t t t a t t a c t g t

660 t g g g t a a g a g t t c c g a a a t a g a a t c t t a g t g t a c a a a a c a a a a a t t g c a t c a t t a g a g a t a c c c a t t c t c a a g g c t t t a t c t t a g a a t c a c a t g t t t t g t t t t t a a c g t a g t a a t c t c t a

720 AmpR promoter c c c c a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t g g g g t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a

780 1 5 10 signal sequence MSIQHFRVALIPFF AmpR promoter AmpR MSIQHFRVALIPFF a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c

840 15 20 signal sequence AAFCLPVFA 25 30 HPETLVKVKDA AmpR AAFCLPVFAHPETLVKVKDA c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c

900 35 40 45 50 EDQLGARVGYIELDLNSGKI AmpR EDQLGARVGYIELDLNSGKI a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g

960 55 60 65 70 LESFRPEERFPMMSTFKVLL AmpR C G A T A Amp-R LESFRPEERFPMMSTFKVLL t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a

BsaHI 1020 75 80 85 90 CGAVLSRIDAGQEQLGRRIH AmpR C A C C G C G C C A T A A T A Amp-R CGAVLSRIDAGQEQLGRRIH g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a

1080 95 100 105 110 YSQNDLVEYSPVTEKHLTDG AmpR YSQNDLVEYSPVTEKHLTDG a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t

1140 115 120 125 130 MTVRELCSAAITMSDNTAAN AmpR MTVRELCSAAITMSDNTAAN t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a

1200 135 140 145 150 LLLTTIGGPKELTAFLHNMG AmpR LLLTTIGGPKELTAFLHNMG t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c

1260 155 160 165 170 DHVTRLDRWEPELNEAIPND AmpR DHVTRLDRWEPELNEAIPND a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c

1320 175 180 185 190 ERDTTMPVAMATTLRKLLTG AmpR ERDTTMPVAMATTLRKLLTG a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c

1380 195 200 205 210 ELLTLASRQQLIDWMEADKV AmpR ELLTLASRQQLIDWMEADKV a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c

1440 215 220 225 230 AGPLLRSALPAGWFIADKSG AmpR AGPLLRSALPAGWFIADKSG c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c

1500 235 240 245 250 AGERGSRGIIAALGPDGKPS AmpR AGERGSRGIIAALGPDGKPS c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g

AhdI 1560 255 260 265 270 RIVVIYTTGSQATMDERNRQ AmpR RIVVIYTTGSQATMDERNRQ g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t

1620 275 280 285 IAEIGASLIKHW* AmpR IAEIGASLIKHW* t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a

1680 a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g

1740 t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c

1800 ori a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a

1860 ori g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g

1920 ori c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g

1980 ori t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c

2040 ori c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a

2100 ori t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a

2160 ori g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g

2220 ori t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t

2280 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t

2340 ori g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c

2400 ori g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a

2460 g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t

2520 A G C G A G T C A G L4440 c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c

2580 T G A G C G A G L4440 t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t

2640 t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c

2700 -35 CAP binding site lac promoter c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c

2760 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g

Eco53kISacI 2820 T3 promoter G C A A T T A A C C C T C A C T A A A G G T3 a t g a t t a c g c c a a g c g c g c a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g a g c t c a t a c t a c t a a t g c g g t t c g c g c g t t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c t c g a g t a t g

2880 c a g t t a c a a t t a g t a t t a c a a t g g t t t a c c a a g t a c a a a t t a t g t a a t a t g g t t a t a a t t g t c a a t g t t a a t c a t a a t g t t a c c a a a t g g t t c a t g t t t a a t a c a t t a t a c c a a t a t t a a

2940 g g c t t a t a t g t a t g g a g g t a g g g a a c t g c a g g t t a t t g t t c t g t t a a a c t a c c a g t a a t g c c g a a t a t a c a t a c c t c c a t c c c t t g a c g t c c a a t a a c a a g a c a a t t t g a t g g t c a t t a c

3000 c a a g a c g g c t a a g t c a t a t g a c t g c c a c t t t g t c a a t c c t g a a g a a a a t g a c a a t a g a a a g t t c t g c c g a t t c a g t a t a c t g a c g g t g a a a c a g t t a g g a c t t c t t t t a c t g t t a t c t t t

KflI 3060 g t g a t a t a a t a c a c g t g a t a t g c g t a g g a t g g g a c c c a a a g a t a g c c a c a t c a c a t g a t c c a c t a t a t t a t g t g c a c t a t a c g c a t c c t a c c c t g g g t t t c t a t c g g t g t a g t g t a c t a g

3120 t t c a a a a a c c c c g t c a a a a a g g a a t a a a t c g a a g g a a a a a a a a t g c c a c g t g a c t t t g a t a a g t t t t t g g g g c a g t t t t t c c t t a t t t a g c t t c c t t t t t t t t a c g g t g c a c t g a a a c t a

3180 a g t c a a a a t c a g g t t a t a t t a c t a t a c g a t c c c c c a c a c a t a a c c t t t a c c a c a c a c c g c t c a g t t t t a g t c c a a t a t a a t g a t a t g c t a g g g g g t g t g t a t t g g a a a t g g t g t g t g g c g

3240 a c g g g c t g g a c t t a t c c t a t t a g a a a g c g g t g c a g c c a g g g c c a a g a a a a c g c g a a c g a c t g c c c g a c c t g a a t a g g a t a a t c t t t c g c c a c g t c g g t c c c g g t t c t t t t g c g c t t g c t g

3300 g c a g a a a a a a c g t c a a g c a a a a a a a c t g t g g t g t t a t t t a a a g c a t a c a a t t t c c t g c c t c g t c t t t t t t g c a g t t c g t t t t t t t g a c a c c a c a a t a a a t t t c g t a t g t t a a a g g a c g g a

3360 c c c t t t a a t g c t a c g g g g g a t c t a g c a a a t g g g a a a a t a t c a c a t g a c t t t g g c t a g a a g g g g a a a t t a c g a t g c c c c c t a g a t c g t t t a c c c t t t t a t a g t g t a c t g a a a c c g a t c t t c

3420 g g g t g g g a a a a c a g a t a t t t t a g t c a c a t t g t t t g a t t t c a c g t a c t a c a c g a t a c a c t a c c c a c c c t t t t g t c t a t a a a a t c a g t g t a a c a a a c t a a a g t g c a t g a t g t g c t a t g t g a t

3480 c a c t a c a c a a t a c a c t g c a a t a c a c t a c a c t g t a t g g t t c c c t c c g g c c c t g g a t a t g c t g t g a t g t g t t a t g t g a c g t t a t g t g a t g t g a c a t a c c a a g g g a g g c c g g g a c c t a t a c g a

3540 a g c g a a g g a t c c c a a g c c c a t c g a g g a a a t c a t t c a a g a g c a t c t g c a g g t c t c a a a t a c t c g c t t c c t a g g g t t c g g g t a g c t c c t t t a g t a a g t t c t c g t a g a c g t c c a g a g t t t a t g

SphIMfeI 3600 a c t a a g t c c a a a c a c a a a c a g c a c t g a g c t a a t c c a a g a c a c c a a t t g c a t g c c t t c t c a t g a t t c a g g t t t g t g t t t g t c g t g a c t c g a t t a g g t t c t g t g g t t a a c g t a c g g a a g a g t

3660 a t t a g a a c a g t t t a t t a c a c a g c t t a t a a c t g t g c a t c t t g c c a t t c t t t t c g a g a t a g c t a a t c t t g t c a a a t a a t g t g t c g a a t a t t g a c a c g t a g a a c g g t a a g a a a a g c t c t a t c g

BlpI 3720 c a t t t g c a t t t g a t t a a c a t g c t t a g c t c g t t c t c a a g c c a c a g t a a a a t g g a t t g c c t t g t a a a c g t a a a c t a a t t g t a c g a a t c g a g c a a g a g t t c g g t g t c a t t t t a c c t a a c g g a a

3780 t t g a g t t t c c a t c g t t g t a t a a a t a g g c a c t c a t t c c t a t a g c c t t g c t c t t t g g t c t t g a a c t c a a a g g t a g c a a c a t a t t t a t c c g t g a g t a a g g a t a t c g g a a c g a g a a a c c a g a a c

3840 t c t c t g t a a t g g t a a t a g c t g t a a g t c a g g a t a t a a c a c t c c a g a a a a g a a a c a c c t a a c a g a g a c a t t a c c a t t a t c g a c a t t c a g t c c t a t a t t g t g a g g t c t t t t c t t t g t g g a t t g

EcoRITspMIXmaISmaISpeIXbaI 3900 SK primer KS primer T C T A G A A C T A G T G G A T C pBluescriptSK C T A T G G C pBluescriptKS a a t c t a g a a c t a g t g g a t c c c c c g g g c t g c a g g a a t t c g a t a t c a a g c t t a t c g a t a c c g t t a g a t c t t g a t c a c c t a g g g g g c c c g a c g t c c t t a a g c t a t a g t t c g a a t a g c t a t g g c

PaeR7IXhoISalI 3960 KS primer A G C T G G A G C T pBluescriptKS t c g a c c t c g a g a a c a c a a c c c a c a g c t a c c a c c a t c a a c a a t a t t t a t a t a t a t a a c g t a a g c t g g a g c t c t t g t g t t g g g t g t c g a t g g t g g t a g t t g t t a t a a a t a t a t a t a t t g c a t

4020 c a c a t a g a a a t c a c a c a a a c a g a g t a t t t a t t c t t a a c t a c a t g a a c t a c c a t c a g a c c g g t g t a t c t t t a g t g t g t t t g t c t c a t a a a t a a g a a t t g a t g t a c t t g a t g g t a g t c t g g c

SnaBI 4080 t c t g g g c c a c t a t a t a a t g t g c c a t t c a t a a a c g t g a t c a c t t t a c g t a g c a g g c a a c c c a g a c c c g g t g a t a t a t t a c a c g g t a a g t a t t t g c a c t a g t g a a a t g c a t c g t c c g t t g g g

4140 c a g g t g a a a a t t t t t c a g c g a g c t g c c a g a t t g t c a g g t g a a a a a c t g a a a a a a a c t t c t g t c c a c t t t t a a a a a g t c g c t c g a c g g t c t a a c a g t c c a c t t t t t g a c t t t t t t t g a a g a

4200 g g g c g a t g a g c t t g t g g c g g g a a a t t a a g t a t a t a a a g c a g t t a g t t t c t t c t g c t t c t t c c c g c t a c t c g a a c a c c g c c c t t t a a t t c a t a t a t t t c g t c a a t c a a a g a a g a c g a a g a a

4260 g t g g t t c t g g g t a t t c t t g t a a t a t t t g g a a g a a t a g a a a a t c a a g a c t a c t g c c t t t c t c a c c a a g a c c c a t a a g a a c a t t a t a a a c c t t c t t a t c t t t t a g t t c t g a t g a c g g a a a g a

Acc65IKpnI 4320 t t t c a t a t t a t a g a g g a a g a a a t a c g c a c g a a c a c g a t a t a g a g g t a a a g g t a c c c a a t t a a a g t a t a a t a t c t c c t t c t t t a t g c g t g c t t g t g c t a t a t c t c c a t t t c c a t g g g t t a a

4380 T7 promoter M13 fwd G G G A T A T C A C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A M13/pUC Forward c g c c c t a t a g t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g c g g g a t a t c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a

4440 C C C M13/pUC Forward g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a

4500 g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c

4560 f1 ori g c g a a t g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g c t t a c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c

4620 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a

NgoMIVNaeI 4680 f1 ori t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a

4740 f1 ori c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c

4800 f1 ori t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a

4860 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a

4920 f1 ori t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t

4980 f1 ori a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t c c t g a t g c g g t a t t t t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a g g a c t a c g c c a t a a a a

5040 c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a g g g t a a t a a c t g a t a t a a t t a a a t g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t c c c a t t a t t g a c t a t a t t a a t t t a

NsiI 5100 *N URA3 *N t g a a g c t c t a a t t t g t g a g t t t a g t a t a c a t g c a t t t a c t t a t a a t a c a g t t t t t t a g t t a c t t c g a g a t t a a a c a c t c a a a t c a t a t g t a c g t a a a t g a a t a t t a t g t c a a a a a a t c a a

5160 265 260 255 250 QQGCRRLYAEWGAKRYREGE URA3 QQGCRRLYAEWGAKRYREGE t t g c t g g c c g c a t c t t c t c a a a t a t g c t t c c c a g c c t g c t t t t c t g t a a c g t t c a c c c t c a a c g a c c g g c g t a g a a g a g t t t a t a c g a a g g g t c g g a c g a a a a g a c a t t g c a a g t g g g a g

Bpu10I 5220 245 240 235 230 VKADRGKAFLGRGVIIIDSG URA3 VKADRGKAFLGRGVIIIDSG t a c c t t a g c a t c c c t t c c c t t t g c a a a t a g t c c t c t t c c a a c a a t a a t a a t g t c a g a t c c a t g g a a t c g t a g g g a a g g g a a a c g t t t a t c a g g a g a a g g t t g t t a t t a t t a c a g t c t a g g

5280 225 220 215 210 TSVVDDVTRYQQGLADGKDD URA3 TSVVDDVTRYQQGLADGKDD t g t a g a g a c c a c a t c a t c c a c g g t t c t a t a c t g t t g a c c c a a t g c g t c t c c c t t g t c a t c a c a t c t c t g g t g t a g t a g g t g c c a a g a t a t g a c a a c t g g g t t a c g c a g a g g g a a c a g t a g

5340 205 200 195 190 LGVGPTMILWDYGEDRGGMD URA3 LGVGPTMILWDYGEDRGGMD t a a a c c c a c a c c g g g t g t c a t a a t c a a c c a a t c g t a a c c t t c a t c t c t t c c a c c c a t g t c a t t t g g g t g t g g c c c a c a g t a t t a g t t g g t t a g c a t t g g a a g t a g a g a a g g t g g g t a c a g

5400 185 180 175 170 RQAIFGIVFDKDSKAIDVTG URA3 MSTVP RQAIFGIVFDKDSKAIDVTG t c t t t g a g c a a t a a a g c c g a t a a c a a a a t c t t t g t c g c t c t t c g c a a t g t c a a c a g t a c c a g a a a c t c g t t a t t t c g g c t a t t g t t t t a g a a a c a g c g a g a a g c g t t a c a g t t g t c a t g g

5460 165 160 155 150 KTYEGTSLSGKCSLEALMLL URA3 LVYSPVDREPLHDNSANIKR KTYEGTSLSGKCSLEALMLL c t t a g t a t a t t c t c c a g t a g a t a g g g a g c c c t t g c a t g a c a a t t c t g c t a a c a t c a a a a g g a a t c a t a t a a g a g g t c a t c t a t c c c t c g g g a a c g t a c t g t t a a g a c g a t t g t a g t t t t c

PspOMI*StuI 5520 145 140 135 130 GRPEKTVEEAAQKLGSVIGP URA3 PLGSFVTSSAACFKPLTIPG GRPEKTVEEAAQKLGSVIGP g c c t c t a g g t t c c t t t g t t a c t t c t t c t g c c g c c t g c t t c a a a c c g c t a a c a a t a c c t g g c g g a g a t c c a a g g a a a c a a t g a a g a a g a c g g c g g a c g a a g t t t g g c g a t t g t t a t g g a c c

BsmIApaI* 5580 125 120 115 110 GVVGHANTIDAWEAIRYVGA URA3 PTTPCAFVMSAHSAILYTPA GVVGHANTIDAWEAIRYVGA g c c c a c c a c a c c g t g t g c a t t c g t a a t g t c t g c c c a t t c t g c t a t t c t g t a t a c a c c c g c c g g g t g g t g t g g c a c a c g t a a g c a t t a c a g a c g g g t a a g a c g a t a a g a c a t a t g t g g g c g

BstBIBbsI 5640 105 100 95 90 SYQLKVTNGIDAFKRDEFLL URA3 EYCNLTVLPMSANFLSSKSK SYQLKVTNGIDAFKRDEFLL a g a g t a c t g c a a t t t g a c t g t a t t a c c a a t g t c a g c a a a t t t t c t g t c t t c g a a g a g t a a t c t c a t g a c g t t a a a c t g a c a t a a t g g t t a c a g t c g t t t a a a a g a c a g a a g c t t c t c a t t

NcoI 5700 85 80 75 70 FNYKASLAKLPKVTGEMSFD URA3 KLYLADNAFSGLTVPSMEKS FNYKASLAKLPKVTGEMSFD a a a a t t g t a c t t g g c g g a t a a t g c c t t t a g c g g c t t a a c t g t g c c c t c c a t g g a a a a a t c t t t t a a c a t g a a c c g c c t a t t a c g g a a a t c g c c g a a t t g a c a c g g g a g g t a c c t t t t t a g

5760 65 60 55 50 TLIDVHTKLLCIKPGLAEVL URA3 VKISTCVFSKQILGPNASTN TLIDVHTKLLCIKPGLAEVL a g t c a a g a t a t c c a c a t g t g t t t t t a g t a a a c a a a t t t t g g g a c c t a a t g c t t c a a c t a a t c a g t t c t a t a g g t g t a c a c a a a a a t c a t t t g t t t a a a a c c c t g g a t t a c g a a g t t g a t t

XcmI 5820 45 40 35 30 ELLEKTTRVDLSACLNTQKE URA3 SSNSLVVRTSNEAHKFVCFS ELLEKTTRVDLSACLNTQKE c t c c a g t a a t t c c t t g g t g g t a c g a a c a t c c a a t g a a g c a c a c a a g t t t g t t t g c t t t t c g a g g t c a t t a a g g a a c c a c c a t g c t t g t a g g t t a c t t c g t g t g t t c a a a c a a a c g a a a a g

5880 25 20 15 10 HMINFLKAAVPSPHTAAREK URA3 CMILNSLAATGLG* HMINFLKAAVPSPHTAAREK g t g c a t g a t a t t a a a t a g c t t g g c a g c a a c a g g a c t a g g a t g a g t a g c a g c a c g t t c c t t c a c g t a c t a t a a t t t a t c g a a c c g t c g t t g t c c t g a t c c t a c t c a t c g t c g t g c a a g g a a

SbfI 5940 5 1 YTAKSM URA3 URA3 promoter YTAKSM a t a t g t a g c t t t c g a c a t g a t t t a t c t t c g t t t c c t g c a g g t t t t t g t t c t g t g c a g t t g t a t a c a t c g a a a g c t g t a c t a a a t a g a a g c a a a g g a c g t c c a a a a a c a a g a c a c g t c a a c

6000 URA3 promoter g g t t a a g a a t a c t g g g c a a t t t c a t g t t t c t t c a a c a c t a c a t a t g c g t a t a t a t a c c a a c c a a t t c t t a t g a c c c g t t a a a g t a c a a a g a a g t t g t g a t g t a t a c g c a t a t a t a t g g t t

6060 URA3 promoter t c t a a g t c t g t g c t c c t t c c t t c g t t c t t c c t t c t g t t c g g a g a t t a c c g a a t c a a a a a a a g a t t c a g a c a c g a g g a a g g a a g c a a g a a g g a a g a c a a g c c t c t a a t g g c t t a g t t t t t t

6120 URA3 promoter a t t t c a a g g a a a c c g a a a t c a a a a a a a a g a a t a a a a a a a a a a t g a t g a a t t g a a a a g g t g t a a a g t t c c t t t g g c t t t a g t t t t t t t t c t t a t t t t t t t t t t a c t a c t t a a c t t t t c c a c

6180 A T G T T A G A C G A G A C T A C G G C pRS-marker g t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g

6240 c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t

PfoI 6300 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t

3ʹ 5ʹ 6307 c g c g c g a g c g c g c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.