Addgene EKAR2G_design2_mTFP_175_Venus_173 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: EKAR2G_design2_mTFP_175_Venus_173 Sequencing Result

Map View

800 600 400 200 EGFP-C (815 .. 836) End (836) BsrGI (775) BseRI (728) EGFP-N (691 .. 712) EaeI (681) BtgZI (631) BsrFI (602) BssSI BssSαI (572) PasI (562) PfoI * (471) EXFP-R (432 .. 451) MflI * - BstYI (253) KpnI (237) Acc65I (233) EarI (218) HincII (206) AccI (205) SalI (204) RsrII (191) BsaAI (174) HindIII (149) SacII (134) BtgI (131) BsiHKAI (66) SmlI - AvaI - XhoI - PspXI - BsoBI - PaeR7I (65) DraIII (40) Bsu36I (24) Start (0) 8xHis VN173 EKAR2G_design2_mTFP_175_Venus_173 836 bp

Sequence View

DraIIIBsu36IStart (0) 5ʹ 3ʹ 60 * 5 HHHHHHH 8xHis a c c t t c t g a t a g g c a g c c t g c a c c t g a g g t t a a t c a c t t a g t g a t g g t g a t g g t g a t g g t t g g a a g a c t a t c c g t c g g a c g t g g a c t c c a a t t a g t g a a t c a c t a c c a c t a c c a c t a c c a

PaeR7IBsoBIPspXIXhoISmlIAvaIBsiHKAI 120 (in frame with VN173) *EDLELEELKKQLI 1 H 8xHis *EDLELEELKKQLI g g t g c t c g a g t c a g a t a t c t c a t t c g t c a a g t t c a a g t t c t t c a a g t t t t t t t t g a a g g a c c a c g a g c t c a g t c t a t a g a g t a a g c a g t t c a a g t t c a a g a a g t t c a a a a a a a a c t t c c t

BsaAIHindIIIBtgISacII 180 (in frame with VN173) DRARPFQFSLKAKGVPTRPV DRARPFQFSLKAKGVPTRPV t a t c c c g c g c c c g c g g g a a t t g g a a t g a a a g c t t t g c c t t t c c t a c t g g t g t a c g t g g t a a t a g g g c g c g g g c g c c c t t a a c c t t a c t t t c g a a a c g g a a a g g a t g a c c a c a t g c a c c a t

Acc65IKpnIEarISalIAccIHincIIRsrII 240 (in frame with VN173) DPATRQVTSWEETLQDATG E VN173 DPATRQVTSWEETLQDATGE *RYG c g t c a g g c g c g g t c c g c t g t a c c g t c g a c c a c t c t t c a g t c a g t t g g t c a g c g g t a c c c t g c a g t c c g c g c c a g g c g a c a t g g c a g c t g g t g a g a a g t c a g t c a a c c a g t c g c c a t g g g a

BstYIMflI* 300 170 165 160 155 INHRIKFNAKIGNKQKDATI VN173 INHRIKFNAKIGNKQKDATI RHQPPDQLQGQHREEAQRRH c g a t g t t g t g g c g g a t c t t g a a g t t g g c c t t g a t g c c g t t c t t c t g c t t g t c g g c g g t g a g c t a c a a c a c c g c c t a g a a c t t c a a c c g g a a c t a c g g c a a g a a g a c g a a c a g c c g c c a c t

360 150 145 140 135 YVNHSNYNYELKHGLINGDE VN173 YVNHSNYNYELKHGLINGDE YLRQPQQLQVGAQAGPHQRG t a t a g a c g t t g t g g c t g t t g t a g t t g t a c t c c a g c t t g t g c c c c a g g a t g t t g c c g t c c t a t a t c t g c a a c a c c g a c a a c a t c a a c a t g a g g t c g a a c a c g g g g t c c t a c a a c g g c a g g a

420 130 125 120 115 KFDIGKLEIRNVLTDGEFKV VN173 KFDIGKLEIRNVLTDGEFKV GQLRHGEARHPEGPHRGRVE c c t t g a a g t c g a t g c c c t t c a g c t c g a t g c g g t t c a c c a g g g t g t c g c c c t c g a a c t t c a g g a a c t t c a g c t a c g g g a a g t c g a g c t a c g c c a a g t g g t c c c a c a g c g g g a g c t t g a a g t

PfoI* 480 110 105 100 95 EARTKYNGDDKFFITREQVY VN173 G T C T T G T A G T T G C C G T C G T C EXFP-R EARTKYNGDDKFFITREQVY GRRPDQLQRRGQLLHHAGPR c c t c g g c g c g g g t c t t g t a g t t g c c g t c g t c c t t g a a g a a g a t g g t g c g c t c c t g g a c g t g g a g c c g c g c c c a g a a c a t c a a c g g c a g c a g g a a c t t c t t c t a c c a c g c g a g g a c c t g c a

540 90 85 80 75 GEPMASKFFDHQKMHDPYRA VN173 GEPMASKFFDHQKMHDPYRA LRRAHRVQLLRAAEHPRPLP a g c c t t c g g g c a t g g c g g a c t t g a a g a a g t c g t g c t g c t t c a t g t g g t c g g g g t a g c g g g t c g g a a g c c c g t a c c g c c t g a a c t t c t t c a g c a c g a c g a a g t a c a c c a g c c c c a t c g c c c

BssSαIBssSIPasI 600 70 65 60 55 FCMLGYGLTTVLTPWPVPLK VN173 FCMLGYGLTTVLTPWPVPLK RLVDPRLGPHDRPHALARAA c g a a g c a c a t c a g g c c g t a g c c c a g g g t g g t c a c g a g g g t g g g c c a g g g c a c g g g c a g c t g c t t c g t g t a g t c c g g c a t c g g g t c c c a c c a g t g c t c c c a c c c g g t c c c g t g c c c g t c g a

BtgZIBsrFI 660 50 45 40 35 GTTCILKLTLKGYTADGEGE VN173 GTTCILKLTLKGYTADGEGE QRHHLDAEPDAQRLHCRGRG t g c c g g t g g t g c a g a t c a g c t t c a g g g t c a g c t t g c c g t a g g t g g c a t c g c c c t c g c c c t a c g g c c a c c a c g t c t a g t c g a a g t c c c a g t c g a a c g g c a t c c a c c g t a g c g g g a g c g g g a

EaeI 720 30 25 20 15 GSVSFKHGNVDGDLEVLIPV VN173 C G T C G C C G T C C A G C T C G A C C A G EGFP-N GSVSFKHGNVDGDLEVLIPV RRVRQVQPRKRRRGARGPHA c g c c g g a c a c g c t g a a c t t g t g g c c g t t t a c g t c g c c g t c c a g c t c g a c c a g g a t g g g c a g c g g c c t g t g c g a c t t g a a c a c c g g c a a a t g c a g c g g c a g g t c g a g c t g g t c c t a c c c g t

BsrGIBseRI 780 10 5 VGTFLEEGKS 1a V 1 M VN173 VGTFLEEGKSVMGGSGGKYL GGRHVAGRGQEGHWRQWGQV c c a c c c c g g t g a a c a g c t c c t c g c c c t t g c t c a c c a t g c c a c c g c t g c c a c c c t t g t a c a g g t g g g g c c a c t t g t c g a g g a g c g g g a a c g a g t g g t a c g g t g g c g a c g g t g g g a a c a t g t

End (836) 3ʹ 5ʹ 836 G T G C T T G A G G T C G T C C T G G T A C EGFP-C EDMGLTIGAATVFELLVM ARGHRSHDRRRDRVGAPGH g c t c g t c c a t g c c g a g a g t g a t c c c g g c g g c g g t c a c g a a c t c c a g c a g g a c c a t g c g a g c a g g t a c g g c t c t c a c t a g g g c c g c c g c c a g t g c t t g a g g t c g t c c t g g t a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.