Addgene pmyc-GFP-TNRC6A-(810-924) Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pmyc-GFP-TNRC6A-(810-924) Sequencing Result


Map View

800 600 400 200 End (923) BstXI (903) PasI (872) HindIII (865) BsrGI (848) EGFP-C (792 .. 813) BmrI (744) EXFP-R (445 .. 464) PfoI * (419) BssSI - BssSαI (319) BsrFI (289) BtgZI (260) EGFP-N (184 .. 205) BanI (174) BseRI (169) EarI (113) BtgI - StyI - NcoI (95) BmtI (90) NheI (86) BsaI (67) SfcI (66) T7 (54 .. 73) AseI (53) SacI - BanII (9) Eco53kI (7) Start (0) T7 promoter Myc EGFP EGFP pmyc-GFP-TNRC6A-(810-924) 923 bp

Sequence View

AseIStart (0) Eco53kIBanIISacI 60 T7 promoter T A A T A C G T7 a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c

EarINcoIStyIBtgIBmtINheISfcIBsaI 120 1 5 EQKLISE T7 promoter Myc A C T C A C T A T A G G G T7 MEQKLISE a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g c c c a c c a t g g a a c a a a a a c t c a t c t c a g a a t g a g t g a t a t c c c t c t g g g t t c g a c c g a t c g g g t g g t a c c t t g t t t t t g a g t a g a g t c t t

BanIBseRI 180 10 EDL SGG 1 M 1a V 5 10 SKGEELFTGVVP Myc EGFP 1 M 1a V 5 10 SKGEELFTGVVP EGFP EDLSGGMVSKGEELFTGVVP g a g g a t c t g t c c g g c g g c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c c t c c t a g a c a g g c c g c c g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g

240 15 20 25 30 ILVELDGDVNGHKFSVSGEG EGFP 15 20 25 30 ILVELDGDVNGHKFSVSGEG EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N ILVELDGDVNGHKFSVSGEG a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g

BsrFIBtgZI 300 35 40 45 50 EGDATYGKLTLKFICTTGKL EGFP 35 40 45 50 EGDATYGKLTLKFICTTGKL EGFP EGDATYGKLTLKFICTTGKL g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c

BssSαIBssSI 360 55 60 65 70 PVPWPTLVTTLTYGVQCFSR EGFP 55 60 65 70 PVPWPTLVTTLTYGVQCFSR EGFP PVPWPTLVTTLTYGVQCFSR c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g

PfoI* 420 75 80 85 90 YPDHMKQHDFFKSAMPEGYV EGFP 75 80 85 90 YPDHMKQHDFFKSAMPEGYV EGFP YPDHMKQHDFFKSAMPEGYV t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g

480 95 100 105 110 QERTIFFKDDGNYKTRAEVK EGFP 95 100 105 110 QERTIFFKDDGNYKTRAEVK EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R QERTIFFKDDGNYKTRAEVK c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c

540 115 120 125 130 FEGDTLVNRIELKGIDFKED EGFP 115 120 125 130 FEGDTLVNRIELKGIDFKED EGFP FEGDTLVNRIELKGIDFKED t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g

600 135 140 145 150 GNILGHKLEYNYNSHNVYIM EGFP 135 140 145 150 GNILGHKLEYNYNSHNVYIM EGFP GNILGHKLEYNYNSHNVYIM g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c

660 155 160 165 170 ADKQKNGIKVNFKIRHNIED EGFP 155 160 165 170 ADKQKNGIKVNFKIRHNIED EGFP ADKQKNGIKVNFKIRHNIED g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g

720 175 180 185 190 GSVQLADHYQQNTPIGDGPV EGFP 175 180 185 190 GSVQLADHYQQNTPIGDGPV EGFP GSVQLADHYQQNTPIGDGPV g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c

BmrI 780 195 200 205 210 LLPDNHYLSTQSALSKDPNE EGFP 195 200 205 210 LLPDNHYLSTQSALSKDPNE EGFP LLPDNHYLSTQSALSKDPNE c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c

840 215 220 225 230 KRDHMVLLEFVTAAGITLGM EGFP 215 220 225 230 KRDHMVLLEFVTAAGITLGM EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C KRDHMVLLEFVTAAGITLGM a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c

PasIHindIIIBsrGI 900 235 DELYK (in frame with EGFP) SGGKLPWGEPSTPAT EGFP 235 DELYK (in frame with EGFP) SGGKLPWGEPSTPAT EGFP DELYKSGGKLPWGEPSTPAT g a c g a g c t g t a c a a g t c c g g c g g c a a g c t t c c c t g g g g g g a g c c t t c t a c t c c a g c c a c a c t g c t c g a c a t g t t c a g g c c g c c g t t c g a a g g g a c c c c c c t c g g a a g a t g a g g t c g g t g t

End (923) BstXI 923 (in frame with EGFP) TVDNGTS (in frame with EGFP) TVDNGTS TVDNGTS a c t g t g g a t a a t g g t a c t t c a g c t g a c a c c t a t t a c c a t g a a g t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.