Addgene sZF10 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: sZF10 Sequencing Result


Map View

800 600 400 200 End (934) BtgZI (897) BGH-rev (798 .. 815) AvaI - XhoI - BsoBI - PaeR7I (787) BsrBI (786) NotI (781) XcmI (710) PDGFRβ TM domain BsmI (632) EarI (610) HincII (593) AccI (592) SalI (591) PstI (589) SacII (582) MspA1I (581) BfuAI - BspMI (578) AcuI (555) BbsI (543) AgeI BsaWI (516) BpmI (484) PpuMI (454) Bsu36I (450) BsgI (335) NsiI (327) BpuEI (323) BstAPI (322) StuI (305) BglII (258) NaeI (253) SfiI (252) NgoMIV (251) ApaI - BaeGI - Bme1580I (248) PspOMI (244) NdeI (221) EcoP15I (155) StyI - NcoI (151) EcoRV (145) EcoRI (129) BstXI (125) KpnI (90) Acc65I (86) HindIII (80) BsaI (67) T7 (54 .. 73) AseI (53) BtsI - BtsαI (29) TsoI (3) Start (0) T7 promoter Ig κ leader HA FLAG Myc bGH poly(A) signal sZF10 934 bp

Sequence View

AseIBtsαIBtsIStart (0) TsoI 60 T7 promoter T A A T A C G T7 a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c

HindIIIAcc65IKpnIBsaI 120 T7 promoter A C T C A C T A T A G G G T7 a c t c a c t a t a g g g a g a c c c a a g c t t g g t a c c g a g c t c g g a t c c a c t a g t a a c g g c c g c c a t g a g t g a t a t c c c t c t g g g t t c g a a c c a t g g c t c g a g c c t a g g t g a t c a t t g c c g g c g g t

EcoP15INcoIStyIEcoRVBstXIEcoRI 180 1 5 METDTLLLW Ig κ leader METDTLLLW g t g t g c t g g a a t t c g g c t t g g g g a t a t c c a c c a t g g a g a c a g a c a c a c t c c t g c t a t g g g c a c a c g a c c t t a a g c c g a a c c c c t a t a g g t g g t a c c t c t g t c t g t g t g a g g a c g a t a c c c

NdeI 240 10 15 20 VLLLWVPGSTGD 1 5 YPYDVPDY Ig κ leader HA VLLLWVPGSTGDYPYDVPDY t a c t g c t g c t c t g g g t t c c a g g t t c c a c t g g t g a c t a t c c a t a t g a t g t t c c a g a t t a t g a t g a c g a c g a g a c c c a a g g t c c a a g g t g a c c a c t g a t a g g t a t a c t a c a a g g t c t a a t a c

BglIIPspOMIBme1580IBaeGIApaINgoMIVSfiINaeI 300 A (in frame with HA) GAQPARS 1 5 DYKDDDDK (in frame with FLAG) SRPG HA FLAG AGAQPARSDYKDDDDKSRPG c t g g g g c c c a g c c g g c c a g a t c t g a c t a c a a g g a t g a c g a c g a t a a a t c t a g a c c g g g g g g a c c c c g g g t c g g c c g g t c t a g a c t g a t g t t c c t a c t g c t g c t a t t t a g a t c t g g c c c c c

BsgIBstAPIBpuEINsiIStuI 360 (in frame with FLAG) ERPFQCRICMRNFSRQSNLS ERPFQCRICMRNFSRQSNLS a g a g g c c t t t t c a g t g c a g a a t a t g c a t g c g g a a c t t c t c a a g a c a g t c c a a t c t g t c a a t c t c c g g a a a a g t c a c g t c t t a t a c g t a c g c c t t g a a g a g t t c t g t c a g g t t a g a c a g t t

420 (in frame with FLAG) RHTRTHTGEKPFQCRICMRN RHTRTHTGEKPFQCRICMRN g a c a t a c g c g g a c c c a t a c t g g t g a g a a g c c a t t t c a g t g c c g c a t c t g t a t g a g g a a c t c t g t a t g c g c c t g g g t a t g a c c a c t c t t c g g t a a a g t c a c g g c g t a g a c a t a c t c c t t g a

Bsu36IPpuMI 480 (in frame with FLAG) FSRNEHLVLHLRTHTGEKPF FSRNEHLVLHLRTHTGEKPF t c t c t a g a a a t g a g c a c t t g g t t c t c c a c c t g a g g a c c c a c a c t g g a g a a a a a c c a t t c c a g a g a t c t t t a c t c g t g a a c c a a g a g g t g g a c t c c t g g g t g t g a c c t c t t t t t g g t a a g g

BsaWIAgeIBpmI 540 (in frame with FLAG) QCRICMRNFSQKTGLRVHLK QCRICMRNFSQKTGLRVHLK a g t g t c g c a t t t g c a t g c g g a a t t t c a g c c a a a a g a c c g g t t t g c g c g t g c a t c t g a a g a t c a c a g c g t a a a c g t a c g c c t t a a a g t c g g t t t t c t g g c c a a a c g c g c a c g t a g a c t t c t

PstISalIAccIHincIIBspMIBfuAIMspA1ISacIIAcuIBbsI 600 (in frame with FLAG) THLRGSQLVKSELPRLQVD 1 E Myc THLRGSQLVKSELPRLQVDE c a c a t c t g c g c g g a t c c c a a c t a g t c a a a a g t g a a c t g c c g c g g c t g c a g g t c g a c g a a c g t g t a g a c g c g c c t a g g g t t g a t c a g t t t t c a c t t g a c g g c g c c g a c g t c c a g c t g c t t g

BsmIEarI 660 5 10 QKLISEEDL N 1 5 10 AVGQDTQEVI Myc PDGFR β TM domain QKLISEEDLNAVGQDTQEVI a a a a a c t c a t c t c a g a a g a g g a t c t g a a t g c t g t g g g c c a g g a c a c g c a g g a g g t c a t c g t t t t t g a g t a g a g t c t t c t c c t a g a c t t a c g a c a c c c g g t c c t g t g c g t c c t c c a g t a g c

XcmI 720 15 20 25 30 VVPHSLPFKVVVISAILALV PDGFR β TM domain VVPHSLPFKVVVISAILALV t g g t g c c a c a c t c c t t g c c c t t t a a g g t g g t g g t g a t c t c a g c c a t c c t g g c c c t g g t g g a c c a c g g t g t g a g g a a c g g g a a a t t c c a c c a c c a c t a g a g t c g g t a g g a c c g g g a c c a c c

780 35 40 45 VLTIISLIILIMLWQKKPR * PDGFR β TM domain VLTIISLIILIMLWQKKPR* t g c t c a c c a t c a t c t c c c t t a t c a t c c t c a t c a t g c t t t g g c a g a a g a a g c c a c g t t a g g a c g a g t g g t a g t a g a g g g a a t a g t a g g a g t a g t a c g a a a c c g t c t t c t t c g g t g c a a t c c

NotIBsrBIPaeR7IBsoBIXhoIAvaI 840 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev c g g c c g c t c g a g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c g c c g g c g a g c t c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g

BtgZI 900 bGH poly(A) signal c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t

End (934) 934 bGH poly(A) signal t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.