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Addgene

Sequence Analyzer: TAL3272


Map View

2000 1500 1000 500 End (2430) MflI * - BstYI - BamHI (2425) EciI (2406) AvaI - BsoBI (2398) BclI * (2372) NspI (2312) PflMI (2298) HpaI - HincII (2282) ApaI - BanII (534) PspOMI (530) BtsI - BtsαI (506) ApaLI (502) AcuI (481) PvuII (456) BsrBI (435) BpmI - Bpu10I (421) FspI - FspAI (251) Bsu36I (188) KpnI (130) BanI - Acc65I (126) SacII (123) BsmI (113) SV40 NLS ClaI - BspDI (59) BspHI * (51) PsiI (44) MslI (35) NcoI (14) BmtI (11) NheI (7) XbaI (1) Start (0) 3xFLAG TAL3272 2430 bp

Sequence View

BspDIClaIBspHI*PsiIMslIStart (0) XbaINheIBmtINcoI 60 1 5 10 DYKDHDGDYKDHDI 3xFLAG SRASTMDYKDHDGDYKDHDI t c t a g a g c t a g c a c c a t g g a c t a c a a a g a c c a t g a c g g t g a t t a t a a a g a t c a t g a c a t c a g a t c t c g a t c g t g g t a c c t g a t g t t t c t g g t a c t g c c a c t a a t a t t t c t a g t a c t g t a g

BsmI 120 15 20 DYKDDDDK MA 130 PKKKRKV GIH 3xFLAG SV40 NLS DYKDDDDKMAPKKKRKVGIH g a t t a c a a g g a t g a c g a t g a c a a g a t g g c c c c c a a g a a g a a g a g g a a g g t g g g c a t t c a c c t a a t g t t c c t a c t g c t a c t g t t c t a c c g g g g g t t c t t c t t c t c c t t c c a c c c g t a a g t g

SacIIAcc65IBanIKpnI 180 (in frame with SV40 NLS) RGVPMVDLRTLGYSQQQQEK RGVPMVDLRTLGYSQQQQEK c g c g g g g t a c c t a t g g t g g a c t t g a g g a c a c t c g g t t a t t c g c a a c a g c a a c a g g a g a a a g c g c c c c a t g g a t a c c a c c t g a a c t c c t g t g a g c c a a t a a g c g t t g t c g t t g t c c t c t t t

Bsu36I 240 (in frame with SV40 NLS) IKPKVRSTVAQHHEALVGHG IKPKVRSTVAQHHEALVGHG a t c a a g c c t a a g g t c a g g a g c a c c g t c g c g c a a c a c c a c g a g g c g c t t g t g g g g c a t g g c t a g t t c g g a t t c c a g t c c t c g t g g c a g c g c g t t g t g g t g c t c c g c g a a c a c c c c g t a c c g

FspAIFspI 300 (in frame with SV40 NLS) FTHAHIVALSQHPAALGTVA FTHAHIVALSQHPAALGTVA *LVRRRKPRHS t t c a c t c a t g c g c a t a t t g t c g c g c t t t c a c a g c a c c c t g c g g c g c t t g g g a c g g t g g c t a a g t g a g t a c g c g t a t a a c a g c g c g a a a g t g t c g t g g g a c g c c g c g a a c c c t g c c a c c g a

360 (in frame with SV40 NLS) VKYQDMIAALPEATHEAIVG VKYQDMIAALPEATHEAIVG DFVLIHNRGQGFGRVLCNYP g t c a a a t a c c a a g a t a t g a t t g c g g c c c t g c c c g a a g c c a c g c a c g a g g c a a t t g t a g g g c a g t t t a t g g t t c t a t a c t a a c g c c g g g a c g g g c t t c g g t g c g t g c t c c g t t a a c a t c c c

420 (in frame with SV40 NLS) VGKQWSGARALEALLTVAGE VGKQWSGARALEALLTVAGE DTFLPRSRSCKLRQQSHRTL g t c g g t a a a c a g t g g t c g g g a g c g c g a g c a c t t g a g g c g c t g c t g a c t g t g g c g g g t g a g c a g c c a t t t g t c a c c a g c c c t c g c g c t c g t g a a c t c c g c g a c g a c t g a c a c c g c c c a c t c

PvuIIBsrBIBpu10IBpmI 480 (in frame with SV40 NLS) LRGPPLQLDTGQLLKIAKRG LRGPPLQLDTGQLLKIAKRG KPPRRELEVGPLQQLDRLSP c t t a g g g g g c c t c c g c t c c a g c t c g a c a c c g g g c a g c t g c t g a a g a t c g c g a a g a g a g g g g a a t c c c c c g g a g g c g a g g t c g a g c t g t g g c c c g t c g a c g a c t t c t a g c g c t t c t c t c c c

PspOMIBanIIApaIApaLIBtsαIBtsIAcuI 540 (in frame with SV40 NLS) GVTAVEAVHAWRNALTGAPL GVTAVEAVHAWRNALTGAPL SYCRYLCHVGPAIREGPGGQ g g a g t a a c a g c g g t a g a g g c a g t g c a c g c c t g g c g c a a t g c g c t c a c c g g g g c c c c c t t g c c t c a t t g t c g c c a t c t c c g t c a c g t g c g g a c c g c g t t a c g c g a g t g g c c c c g g g g g a a c

600 (in frame with SV40 NLS) NLTPDQVVAIANNNGGKQAL NLTPDQVVAIANNNGGKQAL VQGWVLYDCDRVIIPSLLGQ a a c c t g a c c c c a g a c c a g g t a g t c g c a a t c g c g a a c a a t a a t g g g g g a a a g c a a g c c c t g t t g g a c t g g g g t c t g g t c c a t c a g c g t t a g c g c t t g t t a t t a c c c c c t t t c g t t c g g g a c

660 (in frame with SV40 NLS) ETVQRLLPVLCQDHGLTPEQ ETVQRLLPVLCQDHGLTPEQ FGHLPQQRDKTLVVAKCRLL g a a a c c g t g c a a a g g t t g t t g c c g g t c c t t t g t c a a g a c c a c g g c c t t a c a c c g g a g c a a c t t t g g c a c g t t t c c a a c a a c g g c c a g g a a a c a g t t c t g g t g c c g g a a t g t g g c c t c g t t

720 (in frame with SV40 NLS) VVAIANNNGGKQALETVQRL VVAIANNNGGKQALETVQRL DHGNCIIVTAFLSKLRNLSK g t c g t g g c c a t t g c a a a t a a t a a c g g t g g c a a a c a g g c t c t t g a g a c g g t t c a g a g a c t t c a g c a c c g g t a a c g t t t a t t a t t g c c a c c g t t t g t c c g a g a a c t c t g c c a a g t c t c t g a a

780 (in frame with SV40 NLS) LPVLCQAHGLTPDQVVAIAS LPVLCQAHGLTPDQVVAIAS EWNETLGVPQSGILNYRNRR c t c c c a g t t c t c t g t c a a g c c c a c g g g c t g a c t c c c g a t c a a g t t g t a g c g a t t g c g t c g g a g g g t c a a g a g a c a g t t c g g g t g c c c g a c t g a g g g c t a g t t c a a c a t c g c t a a c g c a g c

840 (in frame with SV40 NLS) NIGGKQALETVQRLLPVLCQ NIGGKQALETVQRLLPVLCQ VNSPFLCQLSDLPEKGHQTL a a c a t t g g a g g g a a a c a a g c a t t g g a g a c t g t c c a a c g g c t c c t t c c c g t g t t g t g t c a a t t g t a a c c t c c c t t t g t t c g t a a c c t c t g a c a g g t t g c c g a g g a a g g g c a c a a c a c a g t t

900 (in frame with SV40 NLS) AHGLTPAQVVAIANNNGGKQ AHGLTPAQVVAIANNNGGKQ GVTQRRCLHDGDGVVVATLL g c c c a c g g t t t g a c g c c t g c a c a a g t g g t c g c c a t c g c c a a c a a c a a c g g c g g t a a g c a g c g g g t g c c a a a c t g c g g a c g t g t t c a c c a g c g g t a g c g g t t g t t g t t g c c g c c a t t c g t c

960 (in frame with SV40 NLS) ALETVQRLLPVLCQDHGLTP ALETVQRLLPVLCQDHGLTP RQFCYLAQQRYQALIMSQGW g c g c t g g a a a c a g t a c a g c g c c t g c t g c c t g t a c t g t g c c a g g a t c a t g g a c t g a c c c c a c g c g a c c t t t g t c a t g t c g c g g a c g a c g g a c a t g a c a c g g t c c t a g t a c c t g a c t g g g g t

1020 (in frame with SV40 NLS) DQVVAIANNNGGKQALETVQ DQVVAIANNNGGKQALETVQ VLYDCDRVIIPSLLGQFGHL g a c c a g g t a g t c g c a a t c g c g a a c a a t a a t g g g g g a a a g c a a g c c c t g g a a a c c g t g c a a c t g g t c c a t c a g c g t t a g c g c t t g t t a t t a c c c c c t t t c g t t c g g g a c c t t t g g c a c g t t

1080 (in frame with SV40 NLS) RLLPVLCQDHGLTPEQVVAI RLLPVLCQDHGLTPEQVVAI PQQRDKTLVVAKCRLLDHGN a g g t t g t t g c c g g t c c t t t g t c a a g a c c a c g g c c t t a c a c c g g a g c a a g t c g t g g c c a t t t c c a a c a a c g g c c a g g a a a c a g t t c t g g t g c c g g a a t g t g g c c t c g t t c a g c a c c g g t a a

1140 (in frame with SV40 NLS) ASNIGGKQALETVQRLLPVL ASNIGGKQALETVQRLLPVL CAVDTAFLSKLRNLSKEWNE g c a a g c a a c a t c g g t g g c a a a c a g g c t c t t g a g a c g g t t c a g a g a c t t c t c c c a g t t c t c c g t t c g t t g t a g c c a c c g t t t g t c c g a g a a c t c t g c c a a g t c t c t g a a g a g g g t c a a g a g

1200 (in frame with SV40 NLS) CQAHGLTPDQVVAIANNNGG CQAHGLTPDQVVAIANNNGG TLGVPQSGILNYRNRIVISP t g t c a a g c c c a c g g g c t g a c t c c c g a t c a a g t t g t a g c g a t t g c g a a t a a c a a t g g a g g g a c a g t t c g g g t g c c c g a c t g a g g g c t a g t t c a a c a t c g c t a a c g c t t a t t g t t a c c t c c c

1260 (in frame with SV40 NLS) KQALETVQRLLPVLCQAHGL KQALETVQRLLPVLCQAHGL FLCQLSDLPEKGHQTLGVTQ a a a c a a g c a t t g g a g a c t g t c c a a c g g c t c c t t c c c g t g t t g t g t c a a g c c c a c g g t t t g t t t g t t c g t a a c c t c t g a c a g g t t g c c g a g g a a g g g c a c a a c a c a g t t c g g g t g c c a a a c

1320 (in frame with SV40 NLS) TPAQVVAIASHDGGKQALET TPAQVVAIASHDGGKQALET RRCLHDGDGAMIATLLRQFC a c g c c t g c a c a a g t g g t c g c c a t c g c c a g c c a t g a t g g c g g t a a g c a g g c g c t g g a a a c a t g c g g a c g t g t t c a c c a g c g g t a g c g g t c g g t a c t a c c g c c a t t c g t c c g c g a c c t t t g t

1380 (in frame with SV40 NLS) VQRLLPVLCQDHGLTPDQVV VQRLLPVLCQDHGLTPDQVV YLAQQRYQALIMSQGWVLYD g t a c a g c g c c t g c t g c c t g t a c t g t g c c a g g a t c a t g g a c t g a c c c c a g a c c a g g t a g t c c a t g t c g c g g a c g a c g g a c a t g a c a c g g t c c t a g t a c c t g a c t g g g g t c t g g t c c a t c a g

1440 (in frame with SV40 NLS) AIASNIGGKQALETVQRLLP AIASNIGGKQALETVQRLLP CDRRVNPSLLGQFGHLPQQR g c a a t c g c g t c g a a c a t t g g g g g a a a g c a a g c c c t g g a a a c c g t g c a a a g g t t g t t g c c g c g t t a g c g c a g c t t g t a a c c c c c t t t c g t t c g g g a c c t t t g g c a c g t t t c c a a c a a c g g c

1500 (in frame with SV40 NLS) VLCQDHGLTPEQVVAIANNN VLCQDHGLTPEQVVAIANNN DKTLVVAKCRLLDHGNCIIV g t c c t t t g t c a a g a c c a c g g c c t t a c a c c g g a g c a a g t c g t g g c c a t t g c a a a t a a t a a c c a g g a a a c a g t t c t g g t g c c g g a a t g t g g c c t c g t t c a g c a c c g g t a a c g t t t a t t a t t g

1560 (in frame with SV40 NLS) GGKQALETVQRLLPVLCQAH GGKQALETVQRLLPVLCQAH TAFLSKLRNLSKEWNETLGV g g t g g c a a a c a g g c t c t t g a g a c g g t t c a g a g a c t t c t c c c a g t t c t c t g t c a a g c c c a c c c a c c g t t t g t c c g a g a a c t c t g c c a a g t c t c t g a a g a g g g t c a a g a g a c a g t t c g g g t g

1620 (in frame with SV40 NLS) GLTPDQVVAIASNIGGKQAL GLTPDQVVAIASNIGGKQAL PQSGILNYRNRRVNSPFLCQ g g g c t g a c t c c c g a t c a a g t t g t a g c g a t t g c g t c g a a c a t t g g a g g g a a a c a a g c a t t g c c c g a c t g a g g g c t a g t t c a a c a t c g c t a a c g c a g c t t g t a a c c t c c c t t t g t t c g t a a c

1680 (in frame with SV40 NLS) ETVQRLLPVLCQAHGLTPAQ ETVQRLLPVLCQAHGLTPAQ LSDLPEKGHQTLGVTQRRCL g a g a c t g t c c a a c g g c t c c t t c c c g t g t t g t g t c a a g c c c a c g g t t t g a c g c c t g c a c a a c t c t g a c a g g t t g c c g a g g a a g g g c a c a a c a c a g t t c g g g t g c c a a a c t g c g g a c g t g t t

1740 (in frame with SV40 NLS) VVAIASHDGGKQALETVQRL VVAIASHDGGKQALETVQRL HDGDGAMIATLLRQFCYLAQ g t g g t c g c c a t c g c c a g c c a t g a t g g c g g t a a g c a g g c g c t g g a a a c a g t a c a g c g c c t g c a c c a g c g g t a g c g g t c g g t a c t a c c g c c a t t c g t c c g c g a c c t t t g t c a t g t c g c g g a c

1800 (in frame with SV40 NLS) LPVLCQDHGLTPDQVVAIAS LPVLCQDHGLTPDQVVAIAS QRYQALIM c t g c c t g t a c t g t g c c a g g a t c a t g g a c t g a c c c c a g a c c a g g t a g t c g c a a t c g c g t c a g a c g g a c a t g a c a c g g t c c t a g t a c c t g a c t g g g g t c t g g t c c a t c a g c g t t a g c g c a g t

1860 (in frame with SV40 NLS) NGGGKQALETVQRLLPVLCQ NGGGKQALETVQRLLPVLCQ a a c g g a g g g g g a a a g c a a g c c c t g g a a a c c g t g c a a a g g t t g t t g c c g g t c c t t t g t c a a t t g c c t c c c c c t t t c g t t c g g g a c c t t t g g c a c g t t t c c a a c a a c g g c c a g g a a a c a g t t

1920 (in frame with SV40 NLS) DHGLTPEQVVAIASNIGGKQ DHGLTPEQVVAIASNIGGKQ g a c c a c g g c c t t a c a c c g g a g c a a g t c g t g g c c a t t g c a a g c a a c a t c g g t g g c a a a c a g c t g g t g c c g g a a t g t g g c c t c g t t c a g c a c c g g t a a c g t t c g t t g t a g c c a c c g t t t g t c

1980 (in frame with SV40 NLS) ALETVQRLLPVLCQAHGLTP ALETVQRLLPVLCQAHGLTP g c t c t t g a g a c g g t t c a g a g a c t t c t c c c a g t t c t c t g t c a a g c c c a c g g g c t g a c t c c c c g a g a a c t c t g c c a a g t c t c t g a a g a g g g t c a a g a g a c a g t t c g g g t g c c c g a c t g a g g g

2040 (in frame with SV40 NLS) DQVVAIASHDGGKQALETVQ DQVVAIASHDGGKQALETVQ g a t c a a g t t g t a g c g a t t g c g t c g c a t g a c g g a g g g a a a c a a g c a t t g g a g a c t g t c c a a c t a g t t c a a c a t c g c t a a c g c a g c g t a c t g c c t c c c t t t g t t c g t a a c c t c t g a c a g g t t

2100 (in frame with SV40 NLS) RLLPVLCQAHGLTPAQVVAI RLLPVLCQAHGLTPAQVVAI c g g c t c c t t c c c g t g t t g t g t c a a g c c c a c g g t t t g a c g c c t g c a c a a g t g g t c g c c a t c g c c g a g g a a g g g c a c a a c a c a g t t c g g g t g c c a a a c t g c g g a c g t g t t c a c c a g c g g t a g

2160 (in frame with SV40 NLS) ASHDGGKQALETVQRLLPVL ASHDGGKQALETVQRLLPVL g c c a g c c a t g a t g g c g g t a a g c a g g c g c t g g a a a c a g t a c a g c g c c t g c t g c c t g t a c t g c g g t c g g t a c t a c c g c c a t t c g t c c g c g a c c t t t g t c a t g t c g c g g a c g a c g g a c a t g a c

2220 (in frame with SV40 NLS) CQDHGLTPEQVVAIASNIGG CQDHGLTPEQVVAIASNIGG t g c c a g g a t c a t g g a c t g a c a c c c g a a c a g g t g g t c g c c a t t g c t t c t a a c a t c g g a g g a a c g g t c c t a g t a c c t g a c t g t g g g c t t g t c c a c c a g c g g t a a c g a a g a t t g t a g c c t c c t

2280 (in frame with SV40 NLS) RPALESIVAQLSRPDPALAA RPALESIVAQLSRPDPALAA c g g c c a g c c t t g g a g t c c a t c g t a g c c c a a t t g t c c a g g c c c g a t c c c g c g t t g g c t g c g g c c g g t c g g a a c c t c a g g t a g c a t c g g g t t a a c a g g t c c g g g c t a g g g c g c a a c c g a c g c

NspIPflMIHincIIHpaI 2340 (in frame with SV40 NLS) LTNDHLVALACLGGRPALDA LTNDHLVALACLGGRPALDA t t a a c g a a t g a c c a t c t g g t g g c g t t g g c a t g t c t t g g t g g a c g a c c c g c g c t c g a t g c a a a t t g c t t a c t g g t a g a c c a c c g c a a c c g t a c a g a a c c a c c t g c t g g g c g c g a g c t a c g t

BsoBIAvaIBclI* 2400 (in frame with SV40 NLS) VKKGLPHAPALIKRTNRRIP VKKGLPHAPALIKRTNRRIP g t c a a a a a g g g t c t g c c t c a t g c t c c c g c a t t g a t c a a a a g a a c c a a c c g g c g g a t t c c c c a g t t t t t c c c a g a c g g a g t a c g a g g g c g t a a c t a g t t t t c t t g g t t g g c c g c c t a a g g g

BamHIBstYIMflI*End (2430) EciI 2430 (in frame with SV40 NLS) ERTSHRVAGS ERTSHRVAGS g a g a g a a c t t c c c a t c g a g t c g c g g g a t c c c t c t c t t g a a g g g t a g c t c a g c g c c c t a g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.