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Addgene

Sequence Analyzer: TAL3132


Map View

2000 1500 1000 500 End (2430) MflI * - BstYI - BamHI (2425) EciI (2406) AvaI - BsoBI (2398) BclI * (2372) NspI (2312) PflMI (2298) HpaI - HincII (2282) BglI (1703) SspI (885) AleI (683) ApaI - BanII (534) PspOMI (530) BtsI - BtsαI (506) ApaLI (502) AcuI (481) NruI * (469) PvuII (456) BsrBI (435) BpmI - Bpu10I (421) FspI - FspAI (251) Bsu36I (188) KpnI (130) BanI - Acc65I (126) SacII (123) BsmI (113) SV40 NLS ClaI - BspDI (59) BspHI * (51) PsiI (44) NcoI (14) BmtI (11) NheI (7) XbaI (1) Start (0) 3xFLAG TAL3132 2430 bp

Sequence View

BspDIClaIBspHI*PsiIStart (0) XbaINheIBmtINcoI 60 1 5 10 DYKDHDGDYKDHDI 3xFLAG SRASTMDYKDHDGDYKDHDI t c t a g a g c t a g c a c c a t g g a c t a c a a a g a c c a t g a c g g t g a t t a t a a a g a t c a t g a c a t c a g a t c t c g a t c g t g g t a c c t g a t g t t t c t g g t a c t g c c a c t a a t a t t t c t a g t a c t g t a g

BsmI 120 15 20 DYKDDDDK MA 130 PKKKRKV GIH 3xFLAG SV40 NLS DYKDDDDKMAPKKKRKVGIH g a t t a c a a g g a t g a c g a t g a c a a g a t g g c c c c c a a g a a g a a g a g g a a g g t g g g c a t t c a c c t a a t g t t c c t a c t g c t a c t g t t c t a c c g g g g g t t c t t c t t c t c c t t c c a c c c g t a a g t g

SacIIAcc65IBanIKpnI 180 (in frame with SV40 NLS) RGVPMVDLRTLGYSQQQQEK RGVPMVDLRTLGYSQQQQEK c g c g g g g t a c c t a t g g t g g a c t t g a g g a c a c t c g g t t a t t c g c a a c a g c a a c a g g a g a a a g c g c c c c a t g g a t a c c a c c t g a a c t c c t g t g a g c c a a t a a g c g t t g t c g t t g t c c t c t t t

Bsu36I 240 (in frame with SV40 NLS) IKPKVRSTVAQHHEALVGHG IKPKVRSTVAQHHEALVGHG a t c a a g c c t a a g g t c a g g a g c a c c g t c g c g c a a c a c c a c g a g g c g c t t g t g g g g c a t g g c t a g t t c g g a t t c c a g t c c t c g t g g c a g c g c g t t g t g g t g c t c c g c g a a c a c c c c g t a c c g

FspAIFspI 300 (in frame with SV40 NLS) FTHAHIVALSQHPAALGTVA FTHAHIVALSQHPAALGTVA t t c a c t c a t g c g c a t a t t g t c g c g c t t t c a c a g c a c c c t g c g g c g c t t g g g a c g g t g g c t a a g t g a g t a c g c g t a t a a c a g c g c g a a a g t g t c g t g g g a c g c c g c g a a c c c t g c c a c c g a

360 (in frame with SV40 NLS) VKYQDMIAALPEATHEAIVG VKYQDMIAALPEATHEAIVG g t c a a a t a c c a a g a t a t g a t t g c g g c c c t g c c c g a a g c c a c g c a c g a g g c a a t t g t a g g g c a g t t t a t g g t t c t a t a c t a a c g c c g g g a c g g g c t t c g g t g c g t g c t c c g t t a a c a t c c c

420 (in frame with SV40 NLS) VGKQWSGARALEALLTVAGE VGKQWSGARALEALLTVAGE g t c g g t a a a c a g t g g t c g g g a g c g c g a g c a c t t g a g g c g c t g c t g a c t g t g g c g g g t g a g c a g c c a t t t g t c a c c a g c c c t c g c g c t c g t g a a c t c c g c g a c g a c t g a c a c c g c c c a c t c

NruI*PvuIIBsrBIBpu10IBpmI 480 (in frame with SV40 NLS) LRGPPLQLDTGQLLKIAKRG LRGPPLQLDTGQLLKIAKRG c t t a g g g g g c c t c c g c t c c a g c t c g a c a c c g g g c a g c t g c t g a a g a t c g c g a a g a g a g g g g a a t c c c c c g g a g g c g a g g t c g a g c t g t g g c c c g t c g a c g a c t t c t a g c g c t t c t c t c c c

PspOMIBanIIApaIApaLIBtsαIBtsIAcuI 540 (in frame with SV40 NLS) GVTAVEAVHAWRNALTGAPL GVTAVEAVHAWRNALTGAPL g g a g t a a c a g c g g t a g a g g c a g t g c a c g c c t g g c g c a a t g c g c t c a c c g g g g c c c c c t t g c c t c a t t g t c g c c a t c t c c g t c a c g t g c g g a c c g c g t t a c g c g a g t g g c c c c g g g g g a a c

600 (in frame with SV40 NLS) NLTPDQVVAIASNGGGKQAL NLTPDQVVAIASNGGGKQAL a a c c t g a c c c c a g a c c a g g t a g t c g c a a t c g c g t c a a a c g g a g g g g g a a a g c a a g c c c t g t t g g a c t g g g g t c t g g t c c a t c a g c g t t a g c g c a g t t t g c c t c c c c c t t t c g t t c g g g a c

660 (in frame with SV40 NLS) ETVQRLLPVLCQDHGLTPEQ ETVQRLLPVLCQDHGLTPEQ g a a a c c g t g c a a a g g t t g t t g c c g g t c c t t t g t c a a g a c c a c g g c c t t a c a c c g g a g c a a c t t t g g c a c g t t t c c a a c a a c g g c c a g g a a a c a g t t c t g g t g c c g g a a t g t g g c c t c g t t

AleI 720 (in frame with SV40 NLS) VVAIASHDGGKQALETVQRL VVAIASHDGGKQALETVQRL g t c g t g g c c a t t g c a t c c c a c g a c g g t g g c a a a c a g g c t c t t g a g a c g g t t c a g a g a c t t c a g c a c c g g t a a c g t a g g g t g c t g c c a c c g t t t g t c c g a g a a c t c t g c c a a g t c t c t g a a

780 (in frame with SV40 NLS) LPVLCQAHGLTPDQVVAIAS LPVLCQAHGLTPDQVVAIAS c t c c c a g t t c t c t g t c a a g c c c a c g g g c t g a c t c c c g a t c a a g t t g t a g c g a t t g c g t c g g a g g g t c a a g a g a c a g t t c g g g t g c c c g a c t g a g g g c t a g t t c a a c a t c g c t a a c g c a g c

840 (in frame with SV40 NLS) HDGGKQALETVQRLLPVLCQ HDGGKQALETVQRLLPVLCQ c a t g a c g g a g g g a a a c a a g c a t t g g a g a c t g t c c a a c g g c t c c t t c c c g t g t t g t g t c a a g t a c t g c c t c c c t t t g t t c g t a a c c t c t g a c a g g t t g c c g a g g a a g g g c a c a a c a c a g t t

SspI 900 (in frame with SV40 NLS) AHGLTPAQVVAIASNIGGKQ AHGLTPAQVVAIASNIGGKQ g c c c a c g g t t t g a c g c c t g c a c a a g t g g t c g c c a t c g c c t c c a a t a t t g g c g g t a a g c a g c g g g t g c c a a a c t g c g g a c g t g t t c a c c a g c g g t a g c g g a g g t t a t a a c c g c c a t t c g t c

960 (in frame with SV40 NLS) ALETVQRLLPVLCQDHGLTP ALETVQRLLPVLCQDHGLTP g c g c t g g a a a c a g t a c a g c g c c t g c t g c c t g t a c t g t g c c a g g a t c a t g g a c t g a c c c c a c g c g a c c t t t g t c a t g t c g c g g a c g a c g g a c a t g a c a c g g t c c t a g t a c c t g a c t g g g g t

1020 (in frame with SV40 NLS) DQVVAIASHDGGKQALETVQ DQVVAIASHDGGKQALETVQ g a c c a g g t a g t c g c a a t c g c g t c a c a t g a c g g g g g a a a g c a a g c c c t g g a a a c c g t g c a a c t g g t c c a t c a g c g t t a g c g c a g t g t a c t g c c c c c t t t c g t t c g g g a c c t t t g g c a c g t t

1080 (in frame with SV40 NLS) RLLPVLCQDHGLTPEQVVAI RLLPVLCQDHGLTPEQVVAI a g g t t g t t g c c g g t c c t t t g t c a a g a c c a c g g c c t t a c a c c g g a g c a a g t c g t g g c c a t t t c c a a c a a c g g c c a g g a a a c a g t t c t g g t g c c g g a a t g t g g c c t c g t t c a g c a c c g g t a a

1140 (in frame with SV40 NLS) ANNNGGKQALETVQRLLPVL ANNNGGKQALETVQRLLPVL g c a a a t a a t a a c g g t g g c a a a c a g g c t c t t g a g a c g g t t c a g a g a c t t c t c c c a g t t c t c c g t t t a t t a t t g c c a c c g t t t g t c c g a g a a c t c t g c c a a g t c t c t g a a g a g g g t c a a g a g

1200 (in frame with SV40 NLS) CQAHGLTPDQVVAIASNGGG CQAHGLTPDQVVAIASNGGG t g t c a a g c c c a c g g g c t g a c t c c c g a t c a a g t t g t a g c g a t t g c g t c c a a c g g t g g a g g g a c a g t t c g g g t g c c c g a c t g a g g g c t a g t t c a a c a t c g c t a a c g c a g g t t g c c a c c t c c c

1260 (in frame with SV40 NLS) KQALETVQRLLPVLCQAHGL KQALETVQRLLPVLCQAHGL a a a c a a g c a t t g g a g a c t g t c c a a c g g c t c c t t c c c g t g t t g t g t c a a g c c c a c g g t t t g t t t g t t c g t a a c c t c t g a c a g g t t g c c g a g g a a g g g c a c a a c a c a g t t c g g g t g c c a a a c

1320 (in frame with SV40 NLS) TPAQVVAIANNNGGKQALET TPAQVVAIANNNGGKQALET a c g c c t g c a c a a g t g g t c g c c a t c g c c a a c a a c a a c g g c g g t a a g c a g g c g c t g g a a a c a t g c g g a c g t g t t c a c c a g c g g t a g c g g t t g t t g t t g c c g c c a t t c g t c c g c g a c c t t t g t

1380 (in frame with SV40 NLS) VQRLLPVLCQDHGLTPDQVV VQRLLPVLCQDHGLTPDQVV g t a c a g c g c c t g c t g c c t g t a c t g t g c c a g g a t c a t g g a c t g a c c c c a g a c c a g g t a g t c c a t g t c g c g g a c g a c g g a c a t g a c a c g g t c c t a g t a c c t g a c t g g g g t c t g g t c c a t c a g

1440 (in frame with SV40 NLS) AIASNGGGKQALETVQRLLP AIASNGGGKQALETVQRLLP g c a a t c g c g t c a a a c g g a g g g g g a a a g c a a g c c c t g g a a a c c g t g c a a a g g t t g t t g c c g c g t t a g c g c a g t t t g c c t c c c c c t t t c g t t c g g g a c c t t t g g c a c g t t t c c a a c a a c g g c

1500 (in frame with SV40 NLS) VLCQDHGLTPEQVVAIASNG VLCQDHGLTPEQVVAIASNG g t c c t t t g t c a a g a c c a c g g c c t t a c a c c g g a g c a a g t c g t g g c c a t t g c a a g c a a t g g g c a g g a a a c a g t t c t g g t g c c g g a a t g t g g c c t c g t t c a g c a c c g g t a a c g t t c g t t a c c c

1560 (in frame with SV40 NLS) GGKQALETVQRLLPVLCQAH GGKQALETVQRLLPVLCQAH g g t g g c a a a c a g g c t c t t g a g a c g g t t c a g a g a c t t c t c c c a g t t c t c t g t c a a g c c c a c c c a c c g t t t g t c c g a g a a c t c t g c c a a g t c t c t g a a g a g g g t c a a g a g a c a g t t c g g g t g

1620 (in frame with SV40 NLS) GLTPDQVVAIASHDGGKQAL GLTPDQVVAIASHDGGKQAL g g g c t g a c t c c c g a t c a a g t t g t a g c g a t t g c g t c g c a t g a c g g a g g g a a a c a a g c a t t g c c c g a c t g a g g g c t a g t t c a a c a t c g c t a a c g c a g c g t a c t g c c t c c c t t t g t t c g t a a c

1680 (in frame with SV40 NLS) ETVQRLLPVLCQAHGLTPAQ ETVQRLLPVLCQAHGLTPAQ g a g a c t g t c c a a c g g c t c c t t c c c g t g t t g t g t c a a g c c c a c g g t t t g a c g c c t g c a c a a c t c t g a c a g g t t g c c g a g g a a g g g c a c a a c a c a g t t c g g g t g c c a a a c t g c g g a c g t g t t

BglI 1740 (in frame with SV40 NLS) VVAIASHDGGKQALETVQRL VVAIASHDGGKQALETVQRL g t g g t c g c c a t c g c c a g c c a t g a t g g c g g t a a g c a g g c g c t g g a a a c a g t a c a g c g c c t g c a c c a g c g g t a g c g g t c g g t a c t a c c g c c a t t c g t c c g c g a c c t t t g t c a t g t c g c g g a c

1800 (in frame with SV40 NLS) LPVLCQDHGLTPDQVVAIAS LPVLCQDHGLTPDQVVAIAS c t g c c t g t a c t g t g c c a g g a t c a t g g a c t g a c c c c a g a c c a g g t a g t c g c a a t c g c g t c a g a c g g a c a t g a c a c g g t c c t a g t a c c t g a c t g g g g t c t g g t c c a t c a g c g t t a g c g c a g t

1860 (in frame with SV40 NLS) NGGGKQALETVQRLLPVLCQ NGGGKQALETVQRLLPVLCQ a a c g g a g g g g g a a a g c a a g c c c t g g a a a c c g t g c a a a g g t t g t t g c c g g t c c t t t g t c a a t t g c c t c c c c c t t t c g t t c g g g a c c t t t g g c a c g t t t c c a a c a a c g g c c a g g a a a c a g t t

1920 (in frame with SV40 NLS) DHGLTPEQVVAIASNGGGKQ DHGLTPEQVVAIASNGGGKQ g a c c a c g g c c t t a c a c c g g a g c a a g t c g t g g c c a t t g c a a g c a a t g g g g g t g g c a a a c a g c t g g t g c c g g a a t g t g g c c t c g t t c a g c a c c g g t a a c g t t c g t t a c c c c c a c c g t t t g t c

1980 (in frame with SV40 NLS) ALETVQRLLPVLCQAHGLTP ALETVQRLLPVLCQAHGLTP g c t c t t g a g a c g g t t c a g a g a c t t c t c c c a g t t c t c t g t c a a g c c c a c g g g c t g a c t c c c c g a g a a c t c t g c c a a g t c t c t g a a g a g g g t c a a g a g a c a g t t c g g g t g c c c g a c t g a g g g

2040 (in frame with SV40 NLS) DQVVAIANNNGGKQALETVQ DQVVAIANNNGGKQALETVQ g a t c a a g t t g t a g c g a t t g c g a a t a a c a a t g g a g g g a a a c a a g c a t t g g a g a c t g t c c a a c t a g t t c a a c a t c g c t a a c g c t t a t t g t t a c c t c c c t t t g t t c g t a a c c t c t g a c a g g t t

2100 (in frame with SV40 NLS) RLLPVLCQAHGLTPAQVVAI RLLPVLCQAHGLTPAQVVAI c g g c t c c t t c c c g t g t t g t g t c a a g c c c a c g g t t t g a c g c c t g c a c a a g t g g t c g c c a t c g c c g a g g a a g g g c a c a a c a c a g t t c g g g t g c c a a a c t g c g g a c g t g t t c a c c a g c g g t a g

2160 (in frame with SV40 NLS) ASNGGGKQALETVQRLLPVL ASNGGGKQALETVQRLLPVL g c c t c g a a t g g c g g c g g t a a g c a g g c g c t g g a a a c a g t a c a g c g c c t g c t g c c t g t a c t g c g g a g c t t a c c g c c g c c a t t c g t c c g c g a c c t t t g t c a t g t c g c g g a c g a c g g a c a t g a c

2220 (in frame with SV40 NLS) CQDHGLTPEQVVAIASNIGG CQDHGLTPEQVVAIASNIGG t g c c a g g a t c a t g g a c t g a c a c c c g a a c a g g t g g t c g c c a t t g c t t c t a a c a t c g g a g g a a c g g t c c t a g t a c c t g a c t g t g g g c t t g t c c a c c a g c g g t a a c g a a g a t t g t a g c c t c c t

2280 (in frame with SV40 NLS) RPALESIVAQLSRPDPALAA RPALESIVAQLSRPDPALAA c g g c c a g c c t t g g a g t c c a t c g t a g c c c a a t t g t c c a g g c c c g a t c c c g c g t t g g c t g c g g c c g g t c g g a a c c t c a g g t a g c a t c g g g t t a a c a g g t c c g g g c t a g g g c g c a a c c g a c g c

NspIPflMIHincIIHpaI 2340 (in frame with SV40 NLS) LTNDHLVALACLGGRPALDA LTNDHLVALACLGGRPALDA t t a a c g a a t g a c c a t c t g g t g g c g t t g g c a t g t c t t g g t g g a c g a c c c g c g c t c g a t g c a a a t t g c t t a c t g g t a g a c c a c c g c a a c c g t a c a g a a c c a c c t g c t g g g c g c g a g c t a c g t

BsoBIAvaIBclI* 2400 (in frame with SV40 NLS) VKKGLPHAPALIKRTNRRIP VKKGLPHAPALIKRTNRRIP g t c a a a a a g g g t c t g c c t c a t g c t c c c g c a t t g a t c a a a a g a a c c a a c c g g c g g a t t c c c c a g t t t t t c c c a g a c g g a g t a c g a g g g c g t a a c t a g t t t t c t t g g t t g g c c g c c t a a g g g

BamHIBstYIMflI*End (2430) EciI 2430 (in frame with SV40 NLS) ERTSHRVAGS ERTSHRVAGS g a g a g a a c t t c c c a t c g a g t c g c g g g a t c c c t c t c t t g a a g g g t a g c t c a g c g c c c t a g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.