Addgene EKAR2G_design2_mTFP_159_Venus_wt Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: EKAR2G_design2_mTFP_159_Venus_wt Sequencing Result


Map View

800 600 400 200 End (838) BsrFI (800) BssSI - BssSαI (771) PasI (761) PstI (751) SfcI (747) BtsI - BtsαI (744) PfoI * (670) EXFP-R (631 .. 650) BaeGI - Bme1580I (541) MflI * - BstYI (452) EGFP-C (282 .. 303) BsrGI (242) KpnI (238) BanI - Acc65I (234) EarI (219) HincII (207) AccI (206) SalI (205) RsrII (192) BsaAI (175) HindIII (150) SacII (135) BtgI (132) BsiHKAI (67) SmlI - AvaI - XhoI - PspXI - BsoBI - PaeR7I (66) DraIII (41) Bsu36I (25) Start (0) 8xHis VC155 EKAR2G_design2_mTFP_159_Venus_wt 838 bp

Sequence View

DraIIIBsu36IStart (0) 60 * 5 HHHHHH 8xHis c a c c t t c t g a t a g g c a g c c t g c a c c t g a g g t t a a t c a c t t a g t g a t g g t g a t g g t g a t g g g t g g a a g a c t a t c c g t c g g a c g t g g a c t c c a a t t a g t g a a t c a c t a c c a c t a c c a c t a c c

PaeR7IBsoBIPspXIXhoISmlIAvaIBsiHKAI 120 (in frame with VC155) *EDLELEELKKQL 1 HH 8xHis *EDLELEELKKQL t g g t g c t c g a g t c a g a t a t c t c a t t c g t c a a g t t c a a g t t c t t c a a g t t t t t t t t g a a g g a c c a c g a g c t c a g t c t a t a g a g t a a g c a g t t c a a g t t c a a g a a g t t c a a a a a a a a c t t c c

BsaAIHindIIIBtgISacII 180 (in frame with VC155) IDRARPFQFSLKAKGVPTRP IDRARPFQFSLKAKGVPTRP a t a t c c c g c g c c c g c g g g a a t t g g a a t g a a a g c t t t g c c t t t c c t a c t g g t g t a c g t g g t t a t a g g g c g c g g g c g c c c t t a a c c t t a c t t t c g a a a c g g a a a g g a t g a c c a c a t g c a c c a

Acc65IBanIKpnIEarISalIAccIHincIIRsrII 240 (in frame with VC155) VDPATRQVTSWEETLQDATG VC155 VDPATRQVTSWEETLQDATG a c g t c a g g c g c g g t c c g c t g t a c c g t c g a c c a c t c t t c a g t c a g t t g g t c a g c g g t a c c c t g c a g t c c g c g c c a g g c g a c a t g g c a g c t g g t g a g a a g t c a g t c a a c c a g t c g c c a t g g g

BsrGI 300 235 230 225 220 KYLEDMGLTIGAATVFELLV VC155 G T G C T T G A G G T C G T C C T G G EGFP-C KYLEDMGLTIGAATVFELLV t t g t a c a g c t c g t c c a t g c c g a g a g t g a t c c c g g c g g c g g t c a c g a a c t c c a g c a g g a c c a a c a t g t c g a g c a g g t a c g g c t c t c a c t a g g g c c g c c g c c a g t g c t t g a g g t c g t c c t g g

360 215 210 205 200 MHDRKENPDKSLASQYSLYH VC155 T A C EGFP-C MHDRKENPDKSLASQYSLYH a t g t g a t c g c g c t t c t c g t t g g g g t c t t t g c t c a g g g c g g a c t g g t a g c t c a g g t a g t g g t a c a c t a g c g c g a a g a g c a a c c c c a g a a a c g a g t c c c g c c t g a c c a t c g a g t c c a t c a c c

420 195 190 185 180 NDPLLVPGDGIPTNQQYHDA VC155 NDPLLVPGDGIPTNQQYHDA t t g t c g g g c a g c a g c a c g g g g c c g t c g c c g a t g g g g g t g t t c t g c t g g t a g t g g t c g g c g a a c a g c c c g t c g t c g t g c c c c g g c a g c g g c t a c c c c c a c a a g a c g a c c a t c a c c a g c c g c

BstYIMflI* 480 175 170 165 160 LQVGGDEINHRIKFNAKIGN VC155 LQVGGDEINHRIKFNAKIGN a g c t g c a c g c c g c c g t c c t c g a t g t t g t g g c g g a t c t t g a a g t t g g c c t t g a t g c c g t t c t c g a c g t g c g g c g g c a g g a g c t a c a a c a c c g c c t a g a a c t t c a a c c g g a a c t a c g g c a a g

540 155 KQKD VC155 KQKDATIYVNHSNYNYELKH t t c t g c t t g t c g g c g g t g a t a t a g a c g t t g t g g c t g t t g t a g t t g t a c t c c a g c t t g t g c a a g a c g a a c a g c c g c c a c t a t a t c t g c a a c a c c g a c a a c a t c a a c a t g a g g t c g a a c a c g

Bme1580IBaeGI 600 GLINGDEKFDIGKLEIRNVL c c c a g g a t g t t g c c g t c c t c c t t g a a g t c g a t g c c c t t c a g c t c g a t g c g g t t c a c c a g g g g g t c c t a c a a c g g c a g g a g g a a c t t c a g c t a c g g g a a g t c g a g c t a c g c c a a g t g g t c c

660 G T C T T G T A G T T G C C G T C G T C EXFP-R TDGEFKVEARTKYNGDDKFF g t g t c g c c c t c g a a c t t c a c c t c g g c g c g g g t c t t g t a g t t g c c g t c g t c c t t g a a g a a g c a c a g c g g g a g c t t g a a g t g g a g c c g c g c c c a g a a c a t c a a c g g c a g c a g g a a c t t c t t c

PfoI* 720 ITREQVYGEPMASKFFDHQK a t g g t g c g c t c c t g g a c g t a g c c t t c g g g c a t g g c g g a c t t g a a g a a g t c g t g c t g c t t c t a c c a c g c g a g g a c c t g c a t c g g a a g c c c g t a c c g c c t g a a c t t c t t c a g c a c g a c g a a g

BssSαIBssSIPasIPstIBtsαIBtsISfcI 780 M a t g t g g t c g g g g t a g c g g g c g a a g c a c t g c a g g c c g t a g c c c a g g g t g g t c a c g a g g g t g t a c a c c a g c c c c a t c g c c c g c t t c g t g a c g t c c g g c a t c g g g t c c c a c c a g t g c t c c c a c

End (838) BsrFI 838 g g c c a g g c a c g g g c a g c t t g c c g g t g g t g c a g a t c a g c t t c a g g t c a g c t t g c c g t a g c c g g t c c g t g c c c g t c g a a c g g c c a c c a c g t c t a g t c g a a g t c c a g t c g a a c g g c a t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.