Addgene EKAR2G_design1_mTFP_227_Venus_195 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: EKAR2G_design1_mTFP_227_Venus_195 Sequencing Result

Map View

800 600 400 200 End (876) HincII (847) BstAPI (814) AatII (804) ZraI (802) BsaAI (775) PspFI (751) PflMI - BseYI (747) BbsI - BsrFI (743) SmlI (692) BpuEI (677) MmeI (672) MslI (656) AvaI - BsoBI (584) BanII Bsp1286I (478) BstXI (457) BanI (440) MscI (393) BsaI (320) BmtI (300) NheI (296) TatI BsrGI (285) XcmI (271) BamHI (257) Bpu10I (247) PacI (129) AseI (125) PpuMI (57) PfoI (53) Start (0) lac operator p10 promoter 6xHis Myc EKAR2G_design1_mTFP_227_Venus_195 876 bp

Sequence View

PpuMIPfoIStart (0) 5ʹ 3ʹ 60 lac operator p10 promoter a t a c g a c t c a c t a t a g g g g a a t t g t g a g c g g a t a a c a a t t c c c c g g a g t t a a t c c g g g a c t a t g c t g a g t g a t a t c c c c t t a a c a c t c g c c t a t t g t t a a g g g g c c t c a a t t a g g c c c t g

120 p10 promoter c t t t a a t t c a a c c c a a c a c a a t a t a t t a t a g t t a a a t a a g a a t t a t t a t c a a a t c a t t t g g a a a t t a a g t t g g g t t g t g t t a t a t a a t a t c a a t t t a t t c t t a a t a a t a g t t t a g t a a a c

PacIAseI 180 p10 promoter t a t a t t a a t t a a a a t a c t a t a c t g t a a a t t a c a t t t t a t t t a c a a t c a a a g g a g a t a t a c a t a t a a t t a a t t t t a t g a t a t g a c a t t t a a t g t a a a a t a a a t g t t a g t t t c c t c t a t a t g

240 1 5 HHHHHH GSG 1 5 EQKLISEED 6xHis Myc MAHHHHHHGSGEQKLISEED c a t g g c a c a c c a t c a c c a c c a c c a c g g g a g c g g g g a g c a g a a a c t a a t a t c a g a g g a g g a g t a c c g t g t g g t a g t g g t g g t g g t g c c c t c g c c c c t c g t c t t t g a t t a t a g t c t c c t c c t

BmtINheIBsrGITatIXcmIBamHIBpu10I 300 10 L (in frame with Myc) GSGSGSSTDGMDELYKGAS Myc LGSGSGSSTDGMDELYKGAS *SGGGVAHVLQVLTSA t c t t g g c t c a g g t t c a g g a t c c t c c a c c g a c g g c a t g g a c g a g c t g t a c a a g g g t g c t a g a g a a c c g a g t c c a a g t c c t a g g a g g t g g c t g c c g t a c c t g c t c g a c a t g t t c c c a c g a t c

BsaI 360 (in frame with Myc) GGMVSKGEETTMGVIKPDMK GGMVSKGEETTMGVIKPDMK SSHHALALLGCHAYDLGVHL c g g a g g a a t g g t g a g c a a g g g c g a g g a g a c c a c a a t g g g c g t a a t c a a g c c c g a c a t g a a g c c t c c t t a c c a c t c g t t c c c g c t c c t c t g g t g t t a c c c g c a t t a g t t c g g g c t g t a c t t

MscI 420 (in frame with Myc) IKLKMEGNVNGHAFVIEGEG IKLKMEGNVNGHAFVIEGEG DLQLHLAVHIAVGEHDLALA g a t c a a g c t g a a g a t g g a g g g c a a c g t g a a t g g c c a c g c c t t c g t g a t c g a g g g c g a g g g c t a g t t c g a c t t c t a c c t c c c g t t g c a c t t a c c g g t g c g g a a g c a c t a g c t c c c g c t c c c

Bsp1286IBanIIBstXIBanI 480 (in frame with Myc) EGKPYDGTNTINLEVKEGAP EGKPYDGTNTINLEVKEGAP LALGVVAGVGDVQLHLLSGG c g a g g g c a a g c c c t a c g a c g g c a c c a a c a c c a t c a a c c t g g a g g t g a a g g a g g g a g c c c c g c t c c c g t t c g g g a t g c t g c c g t g g t t g t g g t a g t t g g a c c t c c a c t t c c t c c c t c g g g g

540 (in frame with Myc) LPFSYDILTTAFAYGNRAFT LPFSYDILTTAFAYGNRAFT QGEGVVNQGGREGVAVPGEG c c t g c c c t t c t c c t a c g a c a t t c t g a c c a c c g c g t t c g c c t a c g g c a a c a g g g c c t t c a c g g a c g g g a a g a g g a t g c t g t a a g a c t g g t g g c g c a a g c g g a t g c c g t t g t c c c g g a a g t g

BsoBIAvaI 600 (in frame with Myc) KYPDDIPNYFKQSFPEGYSW KYPDDIPNYFKQSFPEGYSW LVGVVDGVVELLGEGLAVRP c a a g t a c c c c g a c g a c a t c c c c a a c t a c t t c a a g c a g t c c t t c c c c g a g g g c t a c t c t t g g t t c a t g g g g c t g c t g t a g g g g t t g a t g a a g t t c g t c a g g a a g g g g c t c c c g a t g a g a a c

MslI 660 (in frame with Myc) ERTMTFEDKGIVKVKSDISM ERTMTFEDKGIVKVKSDISM LAGHGELVLADHLHLGVDGH g g a g c g c a c c a t g a c c t t c g a g g a c a a g g g c a t c g t g a a g g t g a a g t c c g a c a t c t c c a t c c t c g c g t g g t a c t g g a a g c t c c t g t t c c c g t a g c a c t t c c a c t t c a g g c t g t a g a g g t a

SmlIBpuEIMmeI 720 (in frame with Myc) EEDSFIYEIHLKGENFPPNG EEDSFIYEIHLKGENFPPNG LLVGEDVLYVELALVEGGVA g g a g g a g g a c t c c t t c a t c t a c g a g a t a c a c c t c a a g g g c g a g a a c t t c c c c c c c a a c g g c c t c c t c c t g a g g a a g t a g a t g c t c t a t g t g g a g t t c c c g c t c t t g a a g g g g g g g t t g c c

BsaAIBsrFIBbsIBseYIPflMIPspFI 780 (in frame with Myc) PVMQKKTTGWDASTERMYVR PVMQKKTTGWDASTERMYVR GHHLLLGGAPVGGGLPHVHA c c c c g t g a t g c a g a a g a a g a c c a c c g g c t g g g a c g c c t c c a c c g a g a g g a t g t a c g t g c g g g g g c a c t a c g t c t t c t t c t g g t g g c c g a c c c t g c g g a g g t g g c t c t c c t a c a t g c a c g c

BstAPIZraIAatII 840 (in frame with Myc) DGVLKGDVKHKLLLEGGGHH DGVLKGDVKHKLLLEGGGHH VAHQLAVDLVLQQQLAAAVV c g a c g g c g t g c t g a a g g g c g a c g t c a a g c a c a a g c t g c t g c t g g a g g g c g g c g g c c a c c a g c t g c c g c a c g a c t t c c c g c t g c a g t t c g t g t t c g a c g a c g a c c t c c c g c c g c c g g t g g t

End (876) HincII 3ʹ 5ʹ 876 (in frame with Myc) RVDFKTIYRAK RVDFKTIYRAK ANVELGDVPGL c c g c g t t g a c t t c a a g a c c a t c t a c a g g g c c a a g a a g g c g c a a c t g a a g t t c t g g t a g a t g t c c c g g t t c t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.