Addgene pRMH122-LexA-CRY2PHR - Sequence Analyzer Skip to main content
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Sequence Analyzer: pRMH122-LexA-CRY2PHR


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pGEX 3' (9174 .. 9196) pRS-marker (9055 .. 9074) M13 rev XbaI (8813) PaqCI (8464) BmrI (8407) pHybLex-R (8042 .. 8066) MscI (7679) StuI * (7447) BseRI (6956) PspXI (6400) EagI - NotI (6394) XcmI (6327) LexA (6304 .. 6325) PmeI (6282) SnaBI (6178) BclI * (5808) BstBI (5493) PacI (4943) BtgZI (4609) pBRforEco (10 .. 28) SwaI (544) pENTR-R (801 .. 820) NruI (900) Kan-R (885 .. 904) SacII (1117) EcoNI (1155) AsiSI - PvuI (1243) PflMI (1506) L4440 (2506 .. 2523) M13/pUC Reverse (2719 .. 2741) lac operator M13 rev T3 promoter Acc65I (2805) KpnI (2809) AclI (2937) CYC1 (3037 .. 3055) AleI (3728) BmgBI (3772) BglI (4474) pRMH122-LexA-CRY2PHR 9224 bp

Sequence View

5ʹ 3ʹ 60 C G G A G C A C T A T G C G G A T A A pBRforEco g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a

120 CEN/ARS c t t a g g a c g g a t c g c t t g c c t g t a a c t t a c a c g c g c c t c g t a t c t t t t a a t g a t g g a a t a g a a t c c t g c c t a g c g a a c g g a c a t t g a a t g t g c g c g g a g c a t a g a a a a t t a c t a c c t t a t

180 CEN/ARS a t t t g g g a a t t t a c t c t g t g t t t a t t t a t t t t t a t g t t t t g t a t t t g g a t t t t a g a a a g t t a a a c c c t t a a a t g a g a c a c a a a t a a a t a a a a a t a c a a a a c a t a a a c c t a a a a t c t t t c a

240 CEN/ARS a a a t a a a g a a g g t a g a a g a g t t a c g g a a t g a a g a a a a a a a a a t a a a c a a a g g t t t a a a a a t t t a t t t c t t c c a t c t t c t c a a t g c c t t a c t t c t t t t t t t t t a t t t g t t t c c a a a t t t t t

300 CEN/ARS a t t t c a a c a a a a a g c g t a c t t t a c a t a t a t a t t t a t t a g a c a a g a a a a g c a g a t t a a a t a t a a a g t t g t t t t t c g c a t g a a a t g t a t a t a t a a a t a a t c t g t t c t t t t c g t c t a a t t t a t

360 CEN/ARS g a t a t a c a t t c g a t t a a c g a t a a g t a a a a t g t a a a a t c a c a g g a t t t t c g t g t g t g g t c t c t a t a t g t a a g c t a a t t g c t a t t c a t t t t a c a t t t t a g t g t c c t a a a a g c a c a c a c c a g a

420 CEN/ARS t c t a c a c a g a c a a g a t g a a a c a a t t c g g c a t t a a t a c c t g a g a g c a g g a a g a g c a a g a t a a g a t g t g t c t g t t c t a c t t t g t t a a g c c g t a a t t a t g g a c t c t c g t c c t t c t c g t t c t a t

480 CEN/ARS a a a g g t a g t a t t t g t t g g c g a t c c c c c t a g a g t c t t t t a c a t c t t c g g a a a a c a a a a a c t t t t c c a t c a t a a a c a a c c g c t a g g g g g a t c t c a g a a a a t g t a g a a g c c t t t t g t t t t t g a

540 CEN/ARS a t t t t t t c t t t a a t t t c t t t t t t t a c t t t c t a t t t t t a a t t t a t a t a t t t a t a t t a a a a a t a a a a a a g a a a t t a a a g a a a a a a a t g a a a g a t a a a a a t t a a a t a t a t a a a t a t a a t t t t t

SwaI 600 CEN/ARS a t t t a a a t t a t a a t t a t t t t t a t a g c a c g t g a t g a a a a g g a c c c a g g t g g c a c t t t t c g g t a a a t t t a a t a t t a a t a a a a a t a t c g t g c a c t a c t t t t c c t g g g t c c a c c g t g a a a a g c c

660 g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c

720 c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t c t g c a g c t c t g g c c c g t g t c t c a g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a g a c g t c g a g a c c g g g c a c a g a g t

780 a a a t c t c t g a t g t t a c a t t g c a c a a g a t a a a a a t a t a t c a t c a t g a a c a a t a a a a c t g t c t t t a g a g a c t a c a a t g t a a c g t g t t c t a t t t t t a t a t a g t a g t a c t t g t t a t t t t g a c a g

840 1 5 10 MSHIQRETSC KanR G T T C C C C A C A A T A C T C G G T A pENTR-R MSHIQRETSC t g c t t a c a t a a a c a g t a a t a c a a g g g g t g t t a t g a g c c a t a t t c a a c g g g a a a c g t c t t g a c g a a t g t a t t t g t c a t t a t g t t c c c c a c a a t a c t c g g t a t a a g t t g c c c t t t g c a g a a c

NruI 900 15 20 25 30 WRPRLNSNMDADLYGYKWAR KanR C C A T A T T T A C C C G A G C Kan-R WRPRLNSNMDADLYGYKWAR c t g g a g g c c g c g a t t a a a t t c c a a c a t g g a t g c t g a t t t a t a t g g g t a t a a a t g g g c t c g g a c c t c c g g c g c t a a t t t a a g g t t g t a c c t a c g a c t a a a t a t a c c c a t a t t t a c c c g a g c

960 35 40 45 50 DNVGQSGATIFRLYGKPDAP KanR G C T A Kan-R DNVGQSGATIFRLYGKPDAP c g a t a a t g t c g g g c a a t c a g g t g c g a c a a t c t t t c g a t t g t a t g g g a a g c c c g a t g c g c c g c t a t t a c a g c c c g t t a g t c c a c g c t g t t a g a a a g c t a a c a t a c c c t t c g g g c t a c g c g g

1020 55 60 65 70 ELFLKHGKGSVANDVTDEMV KanR ELFLKHGKGSVANDVTDEMV a g a g t t g t t t c t g a a a c a t g g c a a a g g t a g c g t t g c c a a t g a t g t t a c a g a t g a g a t g g t t c t c a a c a a a g a c t t t g t a c c g t t t c c a t c g c a a c g g t t a c t a c a a t g t c t a c t c t a c c a

1080 75 80 85 90 RLNWLTEFMPLPTIKHFIRT KanR RLNWLTEFMPLPTIKHFIRT c a g a c t a a a c t g g c t g a c g g a a t t t a t g c c t c t t c c g a c c a t c a a g c a t t t t a t c c g t a c g t c t g a t t t g a c c g a c t g c c t t a a a t a c g g a g a a g g c t g g t a g t t c g t a a a a t a g g c a t g

SacII 1140 95 100 105 110 PDDAWLLTTAIRGKTAFQVL KanR PDDAWLLTTAIRGKTAFQVL t c c t g a t g a t g c a t g g t t a c t c a c c a c t g c g a t c c g c g g g a a a a c a g c a t t c c a g g t a t t a g g a c t a c t a c g t a c c a a t g a g t g g t g a c g c t a g g c g c c c t t t t g t c g t a a g g t c c a t a a

EcoNI 1200 115 120 125 130 EEYPDSGENIVDALAVFLRR KanR EEYPDSGENIVDALAVFLRR a g a a g a a t a t c c t g a t t c a g g t g a a a a t a t t g t t g a t g c g c t g g c a g t g t t c c t g c g c c g t c t t c t t a t a g g a c t a a g t c c a c t t t t a t a a c a a c t a c g c g a c c g t c a c a a g g a c g c g g c

AsiSIPvuI 1260 135 140 145 150 LHSIPVCNCPFNSDRVFRLA KanR LHSIPVCNCPFNSDRVFRLA g t t g c a t t c g a t t c c t g t t t g t a a t t g t c c t t t t a a c a g c g a t c g c g t a t t t c g t c t c g c c a a c g t a a g c t a a g g a c a a a c a t t a a c a g g a a a a t t g t c g c t a g c g c a t a a a g c a g a g c g

1320 155 160 165 170 QAQSRMNNGLVDASDFDDER KanR QAQSRMNNGLVDASDFDDER t c a g g c g c a a t c a c g a a t g a a t a a c g g t t t g g t t g a t g c g a g t g a t t t t g a t g a c g a g c g a g t c c g c g t t a g t g c t t a c t t a t t g c c a a a c c a a c t a c g c t c a c t a a a a c t a c t g c t c g c

1380 175 180 185 190 NGWPVEQVWKEMHTLLPFSP KanR NGWPVEQVWKEMHTLLPFSP t a a t g g c t g g c c t g t t g a a c a a g t c t g g a a a g a a a t g c a t a c g c t t t t g c c a t t c t c a c c a t t a c c g a c c g g a c a a c t t g t t c a g a c c t t t c t t t a c g t a t g c g a a a a c g g t a a g a g t g g

1440 195 200 205 210 DSVVTHGDFSLDNLIFDEGK KanR DSVVTHGDFSLDNLIFDEGK g g a t t c a g t c g t c a c t c a t g g t g a t t t c t c a c t t g a t a a c c t t a t t t t t g a c g a g g g g a a c c t a a g t c a g c a g t g a g t a c c a c t a a a g a g t g a a c t a t t g g a a t a a a a a c t g c t c c c c t t

1500 215 220 225 230 LIGCIDVGRVGIADRYQDLA KanR LIGCIDVGRVGIADRYQDLA a t t a a t a g g t t g t a t t g a t g t t g g a c g a g t c g g a a t c g c a g a c c g a t a c c a g g a t c t t g c t a a t t a t c c a a c a t a a c t a c a a c c t g c t c a g c c t t a g c g t c t g g c t a t g g t c c t a g a a c g

PflMI 1560 235 240 245 250 ILWNCLGEFSPSLQKRLFQK KanR ILWNCLGEFSPSLQKRLFQK c a t c c t a t g g a a c t g c c t c g g t g a g t t t t c t c c t t c a t t a c a g a a a c g g c t t t t t c a a a a g t a g g a t a c c t t g a c g g a g c c a c t c a a a a g a g g a a g t a a t g t c t t t g c c g a a a a a g t t t t

1620 255 260 265 270 YGIDNPDMNKLQFHLMLDEF KanR YGIDNPDMNKLQFHLMLDEF a t a t g g t a t t g a t a a t c c t g a t a t g a a t a a a t t g c a g t t t c a t t t g a t g c t c g a t g a g t t t a t a c c a t a a c t a t t a g g a c t a t a c t t a t t t a a c g t c a a a g t a a a c t a c g a g c t a c t c a a

1680 F* KanR F* t t t c t a a t c a g a a t t g g t t a a t t g g t t g t a a c a c t g g c a g a g c a t t a c g c t g a c t t g a c g a a a g a t t a g t c t t a a c c a a t t a a c c a a c a t t g t g a c c g t c t c g t a a t g c g a c t g a a c t g c

1740 g g a c g g c g c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c c c t g c c g c g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g

1800 ori g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c

1860 ori c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a

1920 ori c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c

1980 ori t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c

2040 ori c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g

2100 ori t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t

2160 ori c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c a t t g a g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g t a a c t

2220 ori g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g

2280 ori g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g g a a c g c c t g g t a t c t t t a t a g t c c t c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c c t t g c g g a c c a t a g a a a t a t c a g g a

2340 ori g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c c g c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g g c

2400 ori a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a

2460 t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g

2520 A G C G A G T C A G T G A G C L4440 t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g

2580 G A G L4440 g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a

2640 t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a

2700 -35 lac promoter a a t g t g a g t t a c c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c c t t t a c a c t c a a t g g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g g a

2760 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a

Acc65IKpnI 2820 T3 promoter t a c g c c a a g c t c g g a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g t a c c g a t c c c g a g c a t g c g g t t c g a g c c t t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c a t g g c t a g g g c t c g

2880 CYC1 terminator t t t g c a a a t t a a a g c c t t c g a g c g t c c c a a a a c c t t c t c a a g c a a g g t t t t c a g t a t a a t a a a c g t t t a a t t t c g g a a g c t c g c a g g g t t t t g g a a g a g t t c g t t c c a a a a g t c a t a t t a

AclI 2940 CYC1 terminator g t t a c a t g c g t a c a c g c g t c t g t a c a g a a a a a a a a g a a a a a t t t g a a a t a t a a a t a a c g t c a a t g t a c g c a t g t g c g c a g a c a t g t c t t t t t t t t c t t t t t a a a c t t t a t a t t t a t t g c a

3000 CYC1 terminator t c t t a a t a c t a a c a t a a c t a t a a a a a a a t a a a t a g g g a c c t a g a c t t c a g g t t g t c t a a c a g a a t t a t g a t t g t a t t g a t a t t t t t t t a t t t a t c c c t g g a t c t g a a g t c c a a c a g a t t g

3060 CYC1 terminator G C G T G A A T G T A A G C G T G A C CYC1 t c c t t c c t t t t c g g t t a g a g c g g a t g t g g g g g g a g g g c g t g a a t g t a a g c g t g a c a t a a c a g g a a g g a a a a g c c a a t c t c g c c t a c a c c c c c c t c c c g c a c t t a c a t t c g c a c t g t a t t g

3120 CYC1 terminator t a a t t a c a t g a t a t c g a c a a a g g a a a a g g g g c c t g t t t a c t c a c a g g c t t t t t t c a a g t a a t t a a t g t a c t a t a g c t g t t t c c t t t t c c c c g g a c a a a t g a g t g t c c g a a a a a a g t t c a t

3180 g g t a a t t a a g t c g t t t c t g t c t t t t t c c t t c t t c a a c c c a c c a a a g g c c a t c t t g g t a c t c c a t t a a t t c a g c a a a g a c a g a a a a a g g a a g a a g t t g g g t g g t t t c c g g t a g a a c c a t g a

3240 t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a

3300 t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t c a t a g a a a t a a t a c a g a a g t a g a t g t t a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a g t a t c t t t a t t a t g t c t t c a t c t a c a a

3360 g a a t t a g a t t a a a c t g a a g a t a t a t a a t t t a t t g g a a a a t a c a t a g a g c t t t t t g t t g a t c t t a a t c t a a t t t g a c t t c t a t a t a t t a a a t a a c c t t t t a t g t a t c t c g a a a a a c a a c t a

3420 *AILEVVGGAARIKEEALK g c g c t t a a g c g a t c a a t t c a a c a a c a c c a c c a g c a g c t c t g a t t t t t t c t t c a g c c a a c t c g c g a a t t c g c t a g t t a a g t t g t t g t g g t g g t c g t c g a g a c t a a a a a a g a a g t c g g t t g a

3480 SVFRAKVIVPVNPIRGKGLI t g g a g a c g a a t c t a g c t t t g a c g a t a a c t g g a a c a t t t g g a a t t c t a c c c t t a c c c a a g a a c c t c t g c t t a g a t c g a a a c t g c t a t t g a c c t t g t a a a c c t t a a g a t g g g a a t g g g t t c t

3540 KGYGAALTDIVPATEKSASK t c t t a c c g t a a c c g g c t g c c a a a g t g t c a a t a a c t g g a g c a g t t t c c t t a g a a g c a g a t t a g a a t g g c a t t g g c c g a c g g t t t c a c a g t t a t t g a c c t c g t c a a a g g a a t c t t c g t c t a a

3600 LYQDRKDEPILTWLKDLNLV t c a a g t a t t g g t c t c t c t t g t c t t c t g g g a t c a a t g t c c a c a a t t t g t c c a a g t t c a a g a a g t t c a t a a c c a g a g a g a a c a g a a g a c c c t a g t t a c a g g t g t t a a a c a g g t t c a a g t t c t

3660 PKWFHAQQKHFYRMGVKGFY c t g g c t t c c a g a a a t g a g c t t g t t g c t t g t g g a a g t a t c t c a t a c c a a c c t t a c c g a a a t g a c c g a a g g t c t t t a c t c g a a c a a c g a a c a c c t t c a t a g a g t a t g g t t g g a a t g g c t t t a

3720 GPHYKDMNIRHHHQGGAMGR a a c c t g g a t g g t a t t t a t c c a t g t t a a t t c t g t g g t g a t g t t g a c c a c c g g c c a t a c c t c t t g g a c c t a c c a t a a a t a g g t a c a a t t a a g a c a c c a c t a c a a c t g g t g g c c g g t a t g g a g

BmgBIAleI 3780 GGPHKRHKGIRGKGASVHGR t a c c a c c g g g g t g c t t t c t g t g c t t a c c g a t a c g a c c t t t a c c g g c t g a g a c g t g a c c t c a t g g t g g c c c c a c g a a a g a c a c g a a t g g c t a t g c t g g a a a t g g c c g a c t c t g c a c t g g a g

3840 HKRTKTFRSPM t g t g c t t t c t a g t c t t a g t g a a t c t g g a a g g c a t t c t t g a t t a g t t g g a t g a t t g t t c t g a c a c g a a a g a t c a g a a t c a c t t a g a c c t t c c g t a a g a a c t a a t c a a c c t a c t a a c a a g a c

3900 g g a t t t a a t g c a a a a a t c a c t t a a g a a g g a a a a t c a a c g g a g a a a g c a a a c g c c a t c t t a c c t a a a t t a c g t t t t t a g t g a a t t c t t c c t t t t a g t t g c c t c t t t c g t t t g c g g t a g a a t

3960 a a t a t a c g g g a t a c a g a t g a a a g g g t t t g a a c c t a t c t g g a a a a t a g c a t t a a a c a a g c g t t a t a t g c c c t a t g t c t a c t t t c c c a a a c t t g g a t a g a c c t t t t a t c g t a a t t t g t t c g c

4020 a a a a a c t g c g a g g a a a a t t g t t t g c g t c t c t g c g g g c t a t t c a c g c g c c a g a g g a a a a t a t t t t t g a c g c t c c t t t t a a c a a a c g c a g a g a c g c c c g a t a a g t g c g c g g t c t c c t t t t a t

4080 g g a a a a a t a a c a g g g c a t t a g a a a a a t a a t t t t g a t t t t g g t a a t g t g t g g g t c c t g g t g c c t t t t t a t t g t c c c g t a a t c t t t t t a t t a a a a c t a a a a c c a t t a c a c a c c c a g g a c c a c

4140 t a c a g a t g t t a c a t t g g t t a c a g t a c t c t t g t t t t t g c t g t g t t t t t c g a t g a a t c t c c a a t g t c t a c a a t g t a a c c a a t g t c a t g a g a a c a a a a a c g a c a c a a a a a g c t a c t t a g a g g t

4200 a a a t g g t t g t t a g c a c a t g g a a g a g t c a c c g a t g c t a a g t t a t c t c t a t g t a a g c t a c g t t t t a c c a a c a a t c g t g t a c c t t c t c a g t g g c t a c g a t t c a a t a g a g a t a c a t t c g a t g c a

4260 g g c g t g a c t t t t g a t g a a g c c g c a c a a g a g a t a c a g g a t t g g c a a c t g c a a a t a g a a t c t c c g c a c t g a a a a c t a c t t c g g c g t g t t c t c t a t g t c c t a a c c g t t g a c g t t t a t c t t a g a

4320 g g g g a t c c c c c c t c g a g a t c c g g g a t c g a a g a a a t g a t g g t a a a t g a a a t a g g a a a t c a a c c c c t a g g g g g g a g c t c t a g g c c c t a g c t t c t t t a c t a c c a t t t a c t t t a t c c t t t a g t t

4380 g g a g c a t g a a g g c a a a a g a c a a a t a t a a g g g t c g a a c g a a a a a t a a a g t g a a a a g t g t t g c c t c g t a c t t c c g t t t t c t g t t t a t a t t c c c a g c t t g c t t t t t a t t t c a c t t t t c a c a a c

4440 a t a t g a t g t a t t t g g c t t t g c g g c g c c g a a a a a a c g a g t t t a c g c a a t t g c a c a a t c a t g t a t a c t a c a t a a a c c g a a a c g c c g c g g c t t t t t t g c t c a a a t g c g t t a a c g t g t t a g t a c

BglI 4500 c t g a c t c t g t g g c g g a c c c g c g c t c t t g c c g g c c c g g c g a t a a c g c t g g g c g t g a g g c t g g a c t g a g a c a c c g c c t g g g c g c g a g a a c g g c c g g g c c g c t a t t g c g a c c c g c a c t c c g a c

4560 t g c c c g g c g g a g t t t t t t g c g c c t g c a t t t t c c a a g g t t t a c c c t g c g c t a a g g g g c g a g a c g g g c c g c c t c a a a a a a c g c g g a c g t a a a a g g t t c c a a a t g g g a c g c g a t t c c c c g c t c

BtgZI 4620 a t t g g a g a a g c a a t a a g a a t g c c g g t t g g g g t t g c g a t g a t g a c g a c c a c g a c a a c t g g t t a a c c t c t t c g t t a t t c t t a c g g c c a a c c c c a a c g c t a c t a c t g c t g g t g c t g t t g a c c a

4680 g t c a t t a t t t a a g t t g c c g a a a g a a c c t g a g t g c a t t t g c a a c a t g a g t a t a c t a g a a g a c a g t a a t a a a t t c a a c g g c t t t c t t g g a c t c a c g t a a a c g t t g t a c t c a t a t g a t c t t c t

4740 a t g a g c c a a g a c t t g c g a g a c g c g a g t t t g c c g g t g g t g c g a a c a a t a g a g c g a c c a t g a t a c t c g g t t c t g a a c g c t c t g c g c t c a a a c g g c c a c c a c g c t t g t t a t c t c g c t g g t a c t

4800 c c t t g a a g g t g a g a c g c g c a t a a c c g c t a g a g t a c t t t g a a g a g g a a a c a g c a a t a g g g t g g a a c t t c c a c t c t g c g c g t a t t g g c g a t c t c a t g a a a c t t c t c c t t t g t c g t t a t c c c a

4860 t g c t a c c a g t a t a a a t a g a c a g g t a c a t a c a a c a c t g g a a a t g g t t g t c t g t t t g a g t a c a c g a t g g t c a t a t t t a t c t g t c c a t g t a t g t t g t g a c c t t t a c c a a c a g a c a a a c t c a t g

4920 g c t t t c a a t t c a t t t g g g t g t g c a c t t t a t t a t g t t a c a a t a t g g a a g g g a a c t t t a c a c c g a a a g t t a a g t a a a c c c a c a c g t g a a a t a a t a c a a t g t t a t a c c t t c c c t t g a a a t g t g

PacI 4980 t t c t c c t a t g c a c a t a t a t t a a t t a a a g t c c a a t g c t a g t a g a g a a g g g g g g t a a c a c c c a a g a g g a t a c g t g t a t a t a a t t a a t t t c a g g t t a c g a t c a t c t c t t c c c c c c a t t g t g g g

5040 ADH1 promoter c t c c g c g c t c t t t t c c g a t t t t t t t c t a a a c c g t g g a a t a t t t c g g a t a t c c t t t t g t t g g a g g c g c g a g a a a a g g c t a a a a a a a g a t t t g g c a c c t t a t a a a g c c t a t a g g a a a a c a a c

5100 ADH1 promoter t t t c c g g g t g t a c a a t a t g g a c t t c c t c t t t t c t g g c a a c c a a a c c c a t a c a t c g g g a t t a a a g g c c c a c a t g t t a t a c c t g a a g g a g a a a a g a c c g t t g g t t t g g g t a t g t a g c c c t a a

5160 ADH1 promoter c c t a t a a t a c c t t c g t t g g t c t c c c t a a c a t g t a g g t g g c g g a g g g g a g a t a t a c a a t a g g g a t a t t a t g g a a g c a a c c a g a g g g a t t g t a c a t c c a c c g c c t c c c c t c t a t a t g t t a t c

5220 ADH1 promoter a a c a g a t a c c a g a c a a g a c a t a a t g g g c t a a a c a a g a c t a c a c c a a t t a c a c t g c c t c a t t t g t c t a t g g t c t g t t c t g t a t t a c c c g a t t t g t t c t g a t g t g g t t a a t g t g a c g g a g t a

5280 ADH1 promoter t g a t g g t g g t a c a t a a c g a a c t a a t a c t g t a g c c c t a g a c t t g a t a g c c a t c a t c a t a t c a c t a c c a c c a t g t a t t g c t t g a t t a t g a c a t c g g g a t c t g a a c t a t c g g t a g t a g t a t a g

5340 ADH1 promoter g a a g t t t c a c t a c c c t t t t t c c a t t t g c c a t c t a t t g a a g t a a t a a t a g g c g c a t g c a a c c t t c a a a g t g a t g g g a a a a a g g t a a a c g g t a g a t a a c t t c a t t a t t a t c c g c g t a c g t t g

5400 ADH1 promoter t t c t t t t c t t t t t t t t t c t t t t c t c t c t c c c c c g t t g t t g t c t c a c c a t a t c c g c a a t g a a a g a a a a g a a a a a a a a a g a a a a g a g a g a g g g g g c a a c a a c a g a g t g g t a t a g g c g t t a c t

5460 ADH1 promoter c a a a a a a a a t g a t g g a a g a c a c t a a a g g a a a a a a t t a a c g a c a a a g a c a g c a c c a a c a g a g t t t t t t t t a c t a c c t t c t g t g a t t t c c t t t t t t a a t t g c t g t t t c t g t c g t g g t t g t c t

BstBI 5520 ADH1 promoter t g t c g t t g t t c c a g a g c t g a t g a g g g g t a t c t t c g a a c a c a c g a a a c t t t t t c c t t c c t t a c a g c a a c a a g g t c t c g a c t a c t c c c c a t a g a a g c t t g t g t g c t t t g a a a a a g g a a g g a a

5580 ADH1 promoter c a t t c a c g c a c a c t a c t c t c t a a t g a g c a a c g g t a t a c g g c c t t c c t t c c a g t t a c t t g a g t a a g t g c g t g t g a t g a g a g a t t a c t c g t t g c c a t a t g c c g g a a g g a a g g t c a a t g a a c t

5640 ADH1 promoter a t t t g a a a t a a a a a a a g t t t g c c g c t t t g c t a t c a a g t a t a a a t a g a c c t g c a a t t a t t a t a a a c t t t a t t t t t t t c a a a c g g c g a a a c g a t a g t t c a t a t t t a t c t g g a c g t t a a t a a t

5700 ADH1 promoter a t c t t t t g t t t c c t c g t c a t t g t t c t c g t t c c c t t t c t t c c t t g t t t c t t t t t c t g c a c a t a g a a a a c a a a g g a g c a g t a a c a a g a g c a a g g g a a a g a a g g a a c a a a g a a a a a g a c g t g t

5760 ADH1 promoter a t a t t t c a a g c t a t a c c a a g c a t a c a a t c a a c t c c a a g c t t g a a g c a a g c c t c c t g a a a g t a t a a a g t t c g a t a t g g t t c g t a t g t t a g t t g a g g t t c g a a c t t c g t t c g g a g g a c t t t c

BclI* 5820 1 5 10 15 20 DNA-binding domain MKALTARQQEVFDLIRDHIS LexA MKALTARQQEVFDLIRDHIS a t g a a a g c g t t a a c g g c c a g g c a a c a a g a g g t g t t t g a t c t c a t c c g t g a t c a c a t c a g c t a c t t t c g c a a t t g c c g g t c c g t t g t t c t c c a c a a a c t a g a g t a g g c a c t a g t g t a g t c g

5880 25 30 35 40 DNA-binding domain QTGMPPTRAEIAQRLGFRSP LexA QTGMPPTRAEIAQRLGFRSP c a g a c a g g t a t g c c g c c g a c g c g t g c g g a a a t c g c g c a g c g t t t g g g g t t c c g t t c c c c a g t c t g t c c a t a c g g c g g c t g c g c a c g c c t t t a g c g c g t c g c a a a c c c c a a g g c a a g g g g t

5940 45 50 55 60 DNA-binding domain NAAEEHLKALARKGVIEIVS LexA NAAEEHLKALARKGVIEIVS a a c g c g g c t g a a g a a c a t c t g a a g g c g c t g g c a c g c a a a g g c g t t a t t g a a a t t g t t t c c t t g c g c c g a c t t c t t g t a g a c t t c c g c g a c c g t g c g t t t c c g c a a t a a c t t t a a c a a a g g

6000 65 70 75 80 DNA-binding domain GASRGIRLLQEEEEGLPLVG LexA GASRGIRLLQEEEEGLPLVG g g c g c a t c a c g c g g g a t t c g t c t g t t g c a g g a a g a g g a a g a a g g g t t g c c g c t g g t a g g t c c g c g t a g t g c g c c c t a a g c a g a c a a c g t c c t t c t c c t t c t t c c c a a c g g c g a c c a t c c a

6060 DNA-binding domain RVAA 85 90 95 100 GEPLLAQQHIEGHYQV LexA RVAAGEPLLAQQHIEGHYQV c g t g t g g c t g c c g g t g a a c c a c t t c t g g c g c a a c a g c a t a t t g a a g g t c a t t a t c a g g t c g c a c a c c g a c g g c c a c t t g g t g a a g a c c g c g t t g t c g t a t a a c t t c c a g t a a t a g t c c a g

6120 105 110 115 120 DPSLFKPNADFLLRVSGMSM LexA DPSLFKPNADFLLRVSGMSM g a t c c t t c c t t a t t c a a g c c g a a t g c t g a t t t c c t g c t g c g c g t c a g c g g g a t g t c g a t g c t a g g a a g g a a t a a g t t c g g c t t a c g a c t a a a g g a c g a c g c g c a g t c g c c c t a c a g c t a c

SnaBI 6180 125 130 135 140 KDIGIMDGDLLAVHKTQDVR LexA KDIGIMDGDLLAVHKTQDVR a a a g a t a t c g g c a t t a t g g a t g g t g a c t t g c t g g c a g t g c a t a a a a c t c a g g a t g t a c g t t t t c t a t a g c c g t a a t a c c t a c c a c t g a a c g a c c g t c a c g t a t t t t g a g t c c t a c a t g c a

6240 145 150 155 160 NGQVVVARIDDEVTVKRLKK LexA NGQVVVARIDDEVTVKRLKK a a c g g t c a g g t c g t t g t c g c a c g t a t t g a t g a c g a a g t t a c c g t t a a g c g c c t g a a a a a a t t g c c a g t c c a g c a a c a g c g t g c a t a a c t a c t g c t t c a a t g g c a a t t c g c g g a c t t t t t t

PmeI 6300 165 170 175 180 QGNKVELLPENSEFKPIVVD LexA QGNKVELLPENSEFKPIVVD c a g g g c a a t a a a g t c g a a c t g t t g c c a g a a a a t a g c g a g t t t a a a c c a a t t g t c g t a g a t g t c c c g t t a t t t c a g c t t g a c a a c g g t c t t t t a t c g c t c a a a t t t g g t t a a c a g c a t c t a

XcmI 6360 185 190 195 200 LRQQSFTIEGLAVGVIRNGD LexA C G T C A G C A G A G C T T C A C C A T T G LexA LRQQSFTIEGLAVGVIRNGD c t t c g t c a g c a g a g c t t c a c c a t t g a a g g g c t g g c g g t t g g g g t t a t t c g c a a c g g c g a c g a a g c a g t c g t c t c g a a g t g g t a a c t t c c c g a c c g c c a a c c c c a a t a a g c g t t g c c g c t g

PspXIEagINotI 6420 WL (in frame with LexA) EFPGIRRPWRPLESTCSQ LexA Kozak sequence WLEFPGIRRPWRPLESTCSQ t g g c t g g a a t t c c c g g g g a t c c g t c g a c c a t g g c g g c c g c t c g a g t c g a c c t g c a g c c a a a c c g a c c t t a a g g g c c c c t a g g c a g c t g g t a c c g c c g g c g a g c t c a g c t g g a c g t c g g t t

6480 (in frame with LexA) ANSGRISYDLGGS 1 5 MKMDKKT CRY2PHR ANSGRISYDLGGSMKMDKKT g c t a a t t c c g g g c g a a t t t c t t a t g a t t t a g g c g g c a g c a t g a a g a t g g a c a a a a a g a c t c g a t t a a g g c c c g c t t a a a g a a t a c t a a a t c c g c c g t c g t a c t t c t a c c t g t t t t t c t g a

6540 10 15 20 25 IVWFRRDLRIEDNPALAAAA CRY2PHR IVWFRRDLRIEDNPALAAAA a t a g t t t g g t t t a g a a g a g a c c t a a g g a t t g a g g a t a a t c c t g c a t t a g c a g c a g c t g c t t a t c a a a c c a a a t c t t c t c t g g a t t c c t a a c t c c t a t t a g g a c g t a a t c g t c g t c g a c g a

6600 30 35 40 45 HEGSVFPVFIWCPEEEGQFY CRY2PHR HEGSVFPVFIWCPEEEGQFY c a c g a a g g a t c t g t t t t t c c t g t c t t c a t t t g g t g t c c t g a a g a a g a a g g a c a g t t t t a t g t g c t t c c t a g a c a a a a a g g a c a g a a g t a a a c c a c a g g a c t t c t t c t t c c t g t c a a a a t a

6660 50 55 60 65 PGRASRWWMKQSLAHLSQSL CRY2PHR PGRASRWWMKQSLAHLSQSL c c t g g a a g a g c t t c a a g a t g g t g g a t g a a a c a a t c a c t t g c t c a c t t a t c t c a a t c c t t g g g a c c t t c t c g a a g t t c t a c c a c c t a c t t t g t t a g t g a a c g a g t g a a t a g a g t t a g g a a c

6720 70 75 80 85 KALGSDLTLIKTHNTISAIL CRY2PHR KALGSDLTLIKTHNTISAIL a a g g c t c t t g g a t c t g a c c t c a c t t t a a t c a a a a c c c a c a a c a c g a t t t c a g c g a t c t t g t t c c g a g a a c c t a g a c t g g a g t g a a a t t a g t t t t g g g t g t t g t g c t a a a g t c g c t a g a a c

6780 90 95 100 105 DCIRVTGATKVVFNHLYDPV CRY2PHR DCIRVTGATKVVFNHLYDPV g a t t g t a t c c g c g t t a c c g g t g c t a c a a a a g t c g t c t t t a a c c a c c t c t a t g a t c c t g t t c t a a c a t a g g c g c a a t g g c c a c g a t g t t t t c a g c a g a a a t t g g t g g a g a t a c t a g g a c a a

6840 110 115 120 125 SLVRDHTVKEKLVERGISVQ CRY2PHR SLVRDHTVKEKLVERGISVQ t c g t t a g t t c g g g a c c a t a c c g t a a a g g a g a a g c t g g t g g a a c g t g g g a t c t c t g t g c a a a g c a a t c a a g c c c t g g t a t g g c a t t t c c t c t t c g a c c a c c t t g c a c c c t a g a g a c a c g t t

6900 130 135 140 145 SYNGDLLYEPWEIYCEKGKP CRY2PHR SYNGDLLYEPWEIYCEKGKP a g c t a c a a t g g a g a t c t a t t g t a t g a a c c g t g g g a g a t a t a c t g c g a a a a g g g c a a a c c t t c g a t g t t a c c t c t a g a t a a c a t a c t t g g c a c c c t c t a t a t g a c g c t t t t c c c g t t t g g a

BseRI 6960 150 155 160 165 FTSFNSYWKKCLDMSIESVM CRY2PHR FTSFNSYWKKCLDMSIESVM t t t a c g a g t t t c a a t t c t t a c t g g a a g a a a t g c t t a g a t a t g t c g a t t g a a t c c g t t a t g a a a t g c t c a a a g t t a a g a a t g a c c t t c t t t a c g a a t c t a t a c a g c t a a c t t a g g c a a t a c

7020 170 175 180 185 LPPPWRLMPITAAAEAIWAC CRY2PHR LPPPWRLMPITAAAEAIWAC c t t c c t c c t c c t t g g c g g t t g a t g c c a a t a a c t g c a g c g g c t g a a g c g a t t t g g g c g t g t g a a g g a g g a g g a a c c g c c a a c t a c g g t t a t t g a c g t c g c c g a c t t c g c t a a a c c c g c a c a

7080 190 195 200 205 SIEELGLENEAEKPSNALLT CRY2PHR SIEELGLENEAEKPSNALLT t c g a t t g a a g a a c t a g g g c t g g a g a a t g a g g c c g a g a a a c c g a g c a a t g c g t t g t t a a c t a g c t a a c t t c t t g a t c c c g a c c t c t t a c t c c g g c t c t t t g g c t c g t t a c g c a a c a a t t g a

7140 210 215 220 225 RAWSPGWSNADKLLNEFIEK CRY2PHR RAWSPGWSNADKLLNEFIEK a g a g c t t g g t c t c c a g g a t g g a g c a a t g c t g a t a a g t t a c t a a a t g a g t t c a t c g a g a a g t c t c g a a c c a g a g g t c c t a c c t c g t t a c g a c t a t t c a a t g a t t t a c t c a a g t a g c t c t t c

7200 230 235 240 245 QLIDYAKNSKKVVGNSTSLL CRY2PHR QLIDYAKNSKKVVGNSTSLL c a g t t g a t a g a t t a t g c a a a g a a c a g c a a g a a a g t t g t t g g g a a t t c t a c t t c a c t a c t t g t c a a c t a t c t a a t a c g t t t c t t g t c g t t c t t t c a a c a a c c c t t a a g a t g a a g t g a t g a a

7260 250 255 260 265 SPYLHFGEISVRHVFQCARM CRY2PHR SPYLHFGEISVRHVFQCARM t c t c c g t a t c t c c a t t t c g g g g a a a t a a g c g t c a g a c a c g t t t t c c a g t g t g c c c g g a t g a g a g g c a t a g a g g t a a a g c c c c t t t a t t c g c a g t c t g t g c a a a a g g t c a c a c g g g c c t a c

7320 270 275 280 285 KQIIWARDKNSEGEESADLF CRY2PHR KQIIWARDKNSEGEESADLF a a a c a a a t t a t a t g g g c a a g a g a t a a g a a c a g t g a a g g a g a a g a a a g t g c a g a t c t t t t t t t t g t t t a a t a t a c c c g t t c t c t a t t c t t g t c a c t t c c t c t t c t t t c a c g t c t a g a a a a a

7380 290 295 300 305 LRGIGLREYSRYICFNFPFT CRY2PHR LRGIGLREYSRYICFNFPFT c t t a g g g g a a t c g g t t t a a g a g a g t a t t c t c g g t a t a t a t g t t t c a a c t t c c c g t t t a c t g a a t c c c c t t a g c c a a a t t c t c t c a t a a g a g c c a t a t a t a c a a a g t t g a a g g g c a a a t g a

7440 310 315 320 325 HEQSLLSHLRFFPWDADVDK CRY2PHR HEQSLLSHLRFFPWDADVDK c a c g a g c a a t c g t t g t t g a g t c a t c t t c g g t t t t t c c c t t g g g a t g c t g a t g t t g a t a a g g t g c t c g t t a g c a a c a a c t c a g t a g a a g c c a a a a a g g g a a c c c t a c g a c t a c a a c t a t t c

StuI* 7500 330 335 340 345 FKAWRQGRTGYPLVDAGMRE CRY2PHR FKAWRQGRTGYPLVDAGMRE t t c a a g g c c t g g a g a c a a g g c a g g a c c g g t t a t c c g t t g g t g g a t g c c g g a a t g a g a g a g a a g t t c c g g a c c t c t g t t c c g t c c t g g c c a a t a g g c a a c c a c c t a c g g c c t t a c t c t c t c

7560 350 355 360 365 LWATGWMHNRIRVIVSSFAV CRY2PHR LWATGWMHNRIRVIVSSFAV c t t t g g g c t a c c g g a t g g a t g c a t a a c a g a a t a a g a g t g a t t g t t t c a a g c t t t g c t g t g g a a a c c c g a t g g c c t a c c t a c g t a t t g t c t t a t t c t c a c t a a c a a a g t t c g a a a c g a c a c

7620 370 375 380 385 KFLLLPWKWGMKYFWDTLLD CRY2PHR KFLLLPWKWGMKYFWDTLLD a a g t t t c t t c t c c t t c c a t g g a a a t g g g g a a t g a a g t a t t t c t g g g a t a c a c t t t t g g a t t t c a a a g a a g a g g a a g g t a c c t t t a c c c c t t a c t t c a t a a a g a c c c t a t g t g a a a a c c t a

MscI 7680 390 395 400 405 ADLECDILGWQYISGSIPDG CRY2PHR ADLECDILGWQYISGSIPDG g c t g a t t t g g a a t g t g a c a t c c t t g g c t g g c a g t a t a t c t c t g g g a g t a t c c c c g a t g g c c g a c t a a a c c t t a c a c t g t a g g a a c c g a c c g t c a t a t a g a g a c c c t c a t a g g g g c t a c c g

7740 410 415 420 425 HELDRLDNPALQGAKYDPEG CRY2PHR HELDRLDNPALQGAKYDPEG c a c g a g c t t g a t c g c t t g g a c a a t c c c g c g t t a c a a g g c g c c a a a t a t g a c c c a g a a g g t g t g c t c g a a c t a g c g a a c c t g t t a g g g c g c a a t g t t c c g c g g t t t a t a c t g g g t c t t c c a

7800 430 435 440 445 EYIRQWLPELARLPTEWIHH CRY2PHR EYIRQWLPELARLPTEWIHH g a g t a c a t a a g g c a a t g g c t t c c c g a g c t t g c g a g a t t g c c a a c t g a a t g g a t c c a t c a t c t c a t g t a t t c c g t t a c c g a a g g g c t c g a a c g c t c t a a c g g t t g a c t t a c c t a g g t a g t a

7860 450 455 460 465 PWDAPLTVLKASGVELGTNY CRY2PHR PWDAPLTVLKASGVELGTNY c c a t g g g a c g c t c c t t t a a c c g t a c t c a a a g c t t c t g g t g t g g a a c t c g g a a c a a a c t a t g g t a c c c t g c g a g g a a a t t g g c a t g a g t t t c g a a g a c c a c a c c t t g a g c c t t g t t t g a t a

7920 470 475 480 485 AKPIVDIDTARELLAKAISR CRY2PHR AKPIVDIDTARELLAKAISR g c g a a a c c c a t t g t a g a c a t c g a c a c a g c t c g t g a g c t a c t a g c t a a a g c t a t t t c a a g a c g c t t t g g g t a a c a t c t g t a g c t g t g t c g a g c a c t c g a t g a t c g a t t t c g a t a a a g t t c t

7980 490 495 TREAQIMIGAA (in frame with CRY2PHR) ARVDLQPS* CRY2PHR TREAQIMIGAAARVDLQPS* a c c c g t g a a g c a c a g a t c a t g a t c g g a g c a g c a g c c c g g g t c g a c c t g c a g c c a a g c t a a t g g g c a c t t c g t g t c t a g t a c t a g c c t c g t c g t c g g g c c c a g c t g g a c g t c g g t t c g a t t

8040 ADH1 terminator t t c c g g g c g a a t t t c t t a t g a t t t a t g a t t t t t a t t a t t a a a t a a g t t a t a a a a a a a a t a a a g g c c c g c t t a a a g a a t a c t a a a t a c t a a a a a t a a t a a t t t a t t c a a t a t t t t t t t t a t

8100 ADH1 terminator C A C A T A T G T T T A A A A T T T C A C T G A G pHybLex-R a g t g t a t a c a a a t t t t a a a g t g a c t c t t a g g t t t t a a a a c g a a a a t t c t t g t t c t t g a g t t c a c a t a t g t t t a a a a t t t c a c t g a g a a t c c a a a a t t t t g c t t t t a a g a a c a a g a a c t c a

8160 ADH1 terminator a a c t c t t t c c t g t a g g t c a g g t t g c t t t c t c a g g t a t a g c a t g a g g t c g c t c t t a t t g a c t t g a g a a a g g a c a t c c a g t c c a a c g a a a g a g t c c a t a t c g t a c t c c a g c g a g a a t a a c t g

8220 ADH1 terminator c a c a c c t c t a c c g g c a t g c c g g c a a g t g c a c a a a c a a t a c t t a a a t a a a t a c t a c t c a g t g t g t g g a g a t g g c c g t a c g g c c g t t c a c g t g t t t g t t a t g a a t t t a t t t a t g a t g a g t c a

8280 225 220 215 210 *KKANKVFNAIKNSDKVG TRP1 *KKANKVFNAIKNSDKVG a a t a a c c t a t t t c t t a g c a t t t t t g a c g a a a t t t g c t a t t t t g t t a g a g t c t t t t a c a c c t t a t t g g a t a a a g a a t c g t a a a a a c t g c t t t a a a c g a t a a a a c a a t c t c a g a a a a t g t g g

8340 205 200 195 190 NTEVGGSVDVGIVGNLRLAD TRP1 NTEVGGSVDVGIVGNLRLAD a t t t g t c t c c a c a c c t c c g c t t a c a t c a a c a c c a a t a a c g c c a t t t a a t c t a a g c g c a t c t a a a c a g a g g t g t g g a g g c g a a t g t a g t t g t g g t t a t t g c g g t a a a t t a g a t t c g c g t a g

8400 185 180 175 170 GVNEPTLGGALMFHLSEPSE TRP1 GVNEPTLGGALMFHLSEPSE a c c a a c a t t t t c t g g c g t c a g t c c a c c a g c t a a c a t a a a a t g t a a g c t t t c g g g g c t c t c t g g t t g t a a a a g a c c g c a g t c a g g t g g t c g a t t g t a t t t t a c a t t c g a a a g c c c c g a g a g

BmrI 8460 165 160 155 150 QRGVWDSISNWDLLEGTGGA TRP1 QRGVWDSISNWDLLEGTGGA t t g c c t t c c a a c c c a g t c a g a a a t c g a g t t c c a a t c c a a a a g t t c a c c t g t c c c a c c t g c a a c g g a a g g t t g g g t c a g t c t t t a g c t c a a g g t t a g g t t t t c a a g t g g a c a g g g t g g a c g

PaqCI 8520 145 140 135 130 ESDFLPIFSHPKQSAASLLI TRP1 ESDFLPIFSHPKQSAASLLI t t c t g a a t c a a a c a a g g g a a t a a a c g a a t g a g g t t t c t g t g a a g c t g c a c t g a g t a g t a t a a g a c t t a g t t t g t t c c c t t a t t t g c t t a c t c c a a a g a c a c t t c g a c g t g a c t c a t c a t a

8580 125 120 115 110 NCDKPFVLRKIVPLGLFEQY TRP1 NCDKPFVLRKIVPLGLFEQY g t t g c a g t c t t t t g g a a a t a c g a g t c t t t t a a t a a c t g g c a a a c c g a g g a a c t c t t g g t a c a a c g t c a g a a a a c c t t t a t g c t c a g a a a a t t a t t g a c c g t t t g g c t c c t t g a g a a c c a t

8640 105 100 95 90 EQWSEDGHLQVIDIGYDNVL TRP1 EQWSEDGHLQVIDIGYDNVL t t c t t g c c a c g a c t c a t c t c c a t g c a g t t g g a c g a t a t c a a t g c c g t a a t c a t t g a c c a g a a g a a c g g t g c t g a g t a g a g g t a c g t c a a c c t g c t a t a g t t a c g g c a t t a g t a a c t g g t c

8700 85 80 75 70 ALVDEKPQNRFVGVLYKPTG TRP1 ALVDEKPQNRFVGVLYKPTG a g c c a a a a c a t c c t c c t t a g g t t g a t t a c g a a a c a c g c c a a c c a a g t a t t t c g g a g t g c c t c g g t t t t g t a g g a g g a a t c c a a c t a a t g c t t t g t g c g g t t g g t t c a t a a a g c c t c a c g g

8760 65 60 55 50 SSNKYAKVLSSIKRAIVPDI TRP1 SSNKYAKVLSSIKRAIVPDI t g a a c t a t t t t t a t a t g c t t t t a c a a g a c t t g a a a t t t t c c t t g c a a t a a c c g g g t c a a t a c t t g a t a a a a a t a t a c g a a a a t g t t c t g a a c t t t a a a a g g a a c g t t a t t g g c c c a g t t a

XbaI 8820 45 40 35 30 TRKRNPVCIIGLLDADSDLA TRP1 TRKRNPVCIIGLLDADSDLA t g t t c t c t t t c t a t t g g g c a c a c a t a t a a t a c c c a g c a a g t c a g c a t c g g a a t c t a g a g c a c a a g a g a a a g a t a a c c c g t g t g t a t a t t a t g g g t c g t t c a g t c g t a g c c t t a g a t c t c g

8880 25 20 15 10 CEAAETSQLGCVKVLPGSSG TRP1 CEAAETSQLGCVKVLPGSSG a c a t t c t g c g g c c t c t g t g c t c t g c a a g c c g c a a a c t t t c a c c a a t g g a c c a g a a c t a c c t g t a a g a c g c c g g a g a c a c g a g a c g t t c g g c g t t t g a a a g t g g t t a c c t g g t c t t g a t g g

8940 5 1 TFNIVSM TRP1 TRP1 promoter TFNIVSM t g t g a a a t t a a t a a c a g a c a t a c t c c a a g c t g c c t t t g t g t g c t t a a t c a c g t a t a c t c a a c a c t t t a a t t a t t g t c t g t a t g a g g t t c g a c g g a a a c a c a c g a a t t a g t g c a t a t g a g t

9000 TRP1 promoter c g t g c t c a a t a g t c a c c a a t g c c c t c c c t c t t g g c c c t c t c c t t t t c t t t t t t c g a c c g a g c a c g a g t t a t c a g t g g t t a c g g g a g g g a g a a c c g g g a g a g g a a a a g a a a a a a g c t g g c t

9060 TRP1 promoter M13 rev A T G T T A pRS-marker a t t a a t t c g t a a t c a t g g t c a t a g c t g t t t c c t g t c g a t a t g g t g c a c t c t c a g t a c a a t t a a t t a a g c a t t a g t a c c a g t a t c g a c a a a g g a c a g c t a t a c c a c g t g a g a g t c a t g t t a

9120 G A C G A G A C T A C G G C pRS-marker c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g

9180 C C G G G A G pGEX 3' c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c

3ʹ 5ʹ 9224 C T G C A T G T G T C A G A G G pGEX 3' c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.