Addgene pAM1954 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAM1954


Map View

PflMI (10,733) PmlI (10,583) GFP-R (10,289 .. 10,317) BamHI (10,227) SmaI (10,223) TspMI - XmaI (10,221) SwaI (10,169) BspDI - ClaI (9796) SalI (9786) EcoO109I (9484) DraIII (8952) Bsu36I (7211) pBR322ori-F (593 .. 612) PciI (748) L4440 (846 .. 863) pRS-marker (944 .. 963) pGEX 3' (1104 .. 1126) pBRrevBam (1346 .. 1365) SgrAI (1388) BstBI (1552) Neo-F (1682 .. 1701) RsrII (1717) Neo-R (2292 .. 2311) BglII (2396) XcmI (2421) BspEI (4573) ScaI (4933) SpeI (5004) BglI (5091) pAM1954 11,002 bp

Sequence View

5ʹ 3ʹ 60 a g c t c g a a t t g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c g a g c t t a a c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a

120 ori t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g

180 ori t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g

240 ori c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g

300 ori c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g

360 ori c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a

420 ori c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a

480 ori g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a

540 ori a c c t a c a g c g t g a g c a t t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t t g g a t g t c g c a c t c g t a a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a

600 ori G G G A A A C G pBR322ori-F a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c

660 ori C C T G G T A T C T T T pBR322ori-F c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a

720 ori g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a

PciI 780 t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c

840 t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c

900 bom A G C G A G T C A G T G A G C G A G L4440 a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t

960 bom A T G T T A G A C G A G A C T A C pRS-marker c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c

1020 bom G G C pRS-marker c c g c a t a g t t a a g c c a g t a t a c a c t c c g c t a t c g c t a c g t g a c t g g g t c a t g g c t g c g c c g g c g t a t c a a t t c g g t c a t a t g t g a g g c g a t a g c g a t g c a c t g a c c c a g t a c c g a c g c g g

1080 c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g

1140 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g

1200 a c c g a a a c g c g c g a g g c a g c t g c g g t a a a g c t c a t c a g c g t g g t c g t g a a g c g a t t c a c a t g g c t t t g c g c g c t c c g t c g a c g c c a t t t c g a g t a g t c g c a c c a g c a c t t c g c t a a g t g t

1260 g a t g t c t g c c t g t t c a t c c g c g t c c a g c t c g t t g a g t t t c t c c a g a a g c g t t a a t g t c t g c t a c a g a c g g a c a a g t a g g c g c a g g t c g a g c a a c t c a a a g a g g t c t t c g c a a t t a c a g a c

1320 g c t t c t g a t a a a g c g g g c c t g c c a c c a t a c c c a c g c c g a a a c a a g c g c t c a t g a g c c c g a c g a a g a c t a t t t c g c c c g g a c g g t g g t a t g g g t g c g g c t t t g t t c g c g a g t a c t c g g g c t

1380 G G T G A T G T C G G C G A T A T A G G pBRrevBam a g t g g c g a g c c c g a t c t t c c c c a t c g g t g a t g t c g g c g a t a t a g g c g c c a g c a a c c g c a c t c a c c g c t c g g g c t a g a a g g g g t a g c c a c t a c a g c c g c t a t a t c c g c g g t c g t t g g c g t g

SgrAI 1440 c t g t g g c g c c g g t g a t g c c g g t g g g c g a a g a a c t c c a g c a t g a g a t c c c c g c g c t g g a g g g a c a c c g c g g c c a c t a c g g c c a c c c g c t t c t t g a g g t c g t a c t c t a g g g g c g c g a c c t c c

1500 a t c a t c c a g c c g g c g t c c c g g a a a a c g a t t c c g a a g c c c a a c c t t t c a t a g a a g g c g g c g t a g t a g g t c g g c c g c a g g g c c t t t t g c t a a g g c t t c g g g t t g g a a a g t a t c t t c c g c c g c

BstBI 1560 MAGWASLGRSFRTP g t g g a a t c g a a a t c t c g t g a t g g c a g g t t g g g c g t c g c t t g g t c g g t c a t t t c g a a c c c c c a c c t t a g c t t t a g a g c a c t a c c g t c c a a c c c g c a g c g a a c c a g c c a g t a a a g c t t g g g g

1620 265 260 255 250 *FFEDLLRYFAIRQSDP NeoR/KanR ESRSEELVKKAIEGDALRIG *FFEDLLRYFAIRQSDP a g a g t c c c g c t c a g a a g a a c t c g t c a a g a a g g c g a t a g a a g g c g a t g c g c t g c g a a t c g g t c t c a g g g c g a g t c t t c t t g a g c a g t t c t t c c g c t a t c t t c c g c t a c g c g a c g c t t a g c c

1680 245 240 235 230 AAIGYLVLFRDAWEGGLEEA NeoR/KanR SGDTVKHEEAVSPFAAKLFS AAIGYLVLFRDAWEGGLEEA g a g c g g c g a t a c c g t a a a g c a c g a g g a a g c g g t c a g c c c a t t c g c c g c c a a g c t c t t c a g c t c g c c g c t a t g g c a t t t c g t g c t c c t t c g c c a g t c g g g t a a g c g g c g g t t c g a g a a g t c

RsrII 1740 225 220 215 210 IDRTALAIDQYRDAVGLRGC NeoR/KanR T T A T A G T G C C C A T C G G T T G C Neo-F NITGSQRYVLIAVRHTQPAT IDRTALAIDQYRDAVGLRGC c a a t a t c a c g g g t a g c c a a c g c t a t g t c c t g a t a g c g g t c c g c c a c a c c c a g c c g g c c a c g t t a t a g t g c c c a t c g g t t g c g a t a c a g g a c t a t c g c c a g g c g g t g t g g g t c g g c c g g t g

1800 205 200 195 190 DIFGSFRGNEVMINPLCADG NeoR/KanR VDESRKAAIFHHDIRQAGIA DIFGSFRGNEVMINPLCADG a g t c g a t g a a t c c a g a a a a g c g g c c a t t t t c c a c c a t g a t a t t c g g c a a g c a g g c a t c g c t c a g c t a c t t a g g t c t t t t c g c c g g t a a a a g g t g g t a c t a t a a g c c g t t c g t c c g t a g c g

1860 185 180 175 170 HTVVLDEGDPMRAKLRAFLE NeoR/KanR MGHDEILAVGHARLEPGEQF HTVVLDEGDPMRAKLRAFLE c a t g g g t c a c g a c g a g a t c c t c g c c g t c g g g c a t g c g c g c c t t g a g c c t g g c g a a c a g t t g t a c c c a g t g c t g c t c t a g g a g c g g c a g c c c g t a c g c g c g g a a c t c g g a c c g c t t g t c a a

1920 165 160 155 150 APALGQHEEDLDDQDVLGAE NeoR/KanR GWREPLMLFVQIILIDKTGF APALGQHEEDLDDQDVLGAE c g g c t g g c g c g a g c c c c t g a t g c t c t t c g t c c a g a t c a t c c t g a t c g a c a a g a c c g g c t t g c c g a c c g c g c t c g g g g a c t a c g a g a a g c a g g t c t a g t a g g a c t a g c t g t t c t g g c c g a a

1980 145 140 135 130 MRTRAREIRHKAQHDFPCTA NeoR/KanR HPSTCSLDAMFRLVVEWAGS MRTRAREIRHKAQHDFPCTA c c a t c c g a g t a c g t g c t c g c t c g a t g c g a t g t t t c g c t t g g t g g t c g a a t g g g c a g g t a g g g t a g g c t c a t g c a c g a g c g a g c t a c g c t a c a a a g c g a a c c a c c a g c t t a c c c g t c c a t c

2040 125 120 115 110 PDLTHLRRMADAMISVKEAP NeoR/KanR RIKRMQPPHCISHDGYFLGR PDLTHLRRMADAMISVKEAP c c g g a t c a a g c g t a t g c a g c c g c c g c a t t g c a t c a g c c a t g a t g g a t a c t t t c t c g g c a g g g c c t a g t t c g c a t a c g t c g g c g g c g t a a c g t a g t c g g t a c t a c c t a t g a a a g a g c c g t c

2100 105 100 95 90 ALHSSLLDQGPVEGLLLWDR NeoR/KanR SKVR* ALHSSLLDQGPVEGLLLWDR g a g c a a g g t g a g a t g a c a g g a g a t c c t g c c c c g g c a c t t c g c c c a a t a g c a g c c a g t c c c c t c g t t c c a c t c t a c t g t c c t c t a g g a c g g g g c c g t g a a g c g g g t t a t c g t c g g t c a g g g

2160 85 80 75 70 GAETVVDLVAACPVGTTALW NeoR/KanR GAETVVDLVAACPVGTTALW t t c c c g c t t c a g t g a c a a c g t c g a g c a c a g c t g c g c a a g g a a c g c c c g t c g t g g c c a g c c a a g g g c g a a g t c a c t g t t g c a g c t c g t g t c g a c g c g t t c c t t g c g g g c a g c a c c g g t c g g

2220 65 60 55 50 SLRAAEDQLENLAGSLDTKV NeoR/KanR SLRAAEDQLENLAGSLDTKV a c g a t a g c c g c g c t g c c t c g t c c t g c a g t t c a t t c a g g g c a c c g g a c a g g t c g g t c t t g a t g c t a t c g g c g c g a c g g a g c a g g a c g t c a a g t a a g t c c c g t g g c c t g t c c a g c c a g a a c t

2280 45 40 35 30 FLVPRGQASLRFVAADSCGI NeoR/KanR FLVPRGQASLRFVAADSCGI c a a a a a g a a c c g g g c g c c c c t g c g c t g a c a g c c g g a a c a c g g c g g c a t c a g a g c a g c c g a g t t t t t c t t g g c c c g c g g g g a c g c g a c t g t c g g c c t t g t g c c g c c g t a g t c t c g t c g g c t

2340 25 20 15 10 TQQAWDYGFLREVWAAPSGA NeoR/KanR G C C C A G T C A T A G C C G A A T A G Neo-R TQQAWDYGFLREVWAAPSGA t t g t c t g t t g t g c c c a g t c a t a g c c g a a t a g c c t c t c c a c c c a a g c g g c c g g a g a a c c t g a a c a g a c a a c a c g g g t c a g t a t c g g c t t a t c g g a g a g g t g g g t t c g c c g g c c t c t t g g a c

BglII 2400 5 1 HLGDQEIM NeoR/KanR HLGDQEIM c g t g c a a t c c a t c t t g t t c a a t c a t g c g a a a c g a t c c t c a t c c t g t c t c t t g a t c a g a t c g c a c g t t a g g t a g a a c a a g t t a g t a c g c t t t g c t a g g a g t a g g a c a g a g a a c t a g t c t a g

XcmI 2460 t t g a t c c c c t g c g c c a t c a g a t c c t t g g c g g c a a g a a a g c c a t c c a g t t t a c t t t g c a g g a a c t a g g g g a c g c g g t a g t c t a g g a a c c g c c g t t c t t t c g g t a g g t c a a a t g a a a c g t c c

2520 *WLAGWSAIGTRKSDM g c t t c c c a a c c t t a c c a g a g g g c g c c c c a g c t g g c a a t t c c g g t t c g c t t g c t g t c c a t a c g a a g g g t t g g a a t g g t c t c c c g c g g g g t c g a c c g t t a a g g c c a a g c g a a c g a c a g g t a t

2580 FGGLRAIAMYAWQLSGAKEK a a a c c g c c c a g t c t a g c t a t c g c c a t g t a a g c c c a c t g c a a g c t a c c t g c t t t c t c t t t g t t t g g c g g g t c a g a t c g a t a g c g g t a c a t t c g g g t g a c g t t c g a t g g a c g a a a g a g a a a c

2640 RKRKGKDLYGLLQCEDPTLV c g c t t g c g t t t t c c c t t g t c c a g a t a g c c c a g t a g c t g a c a t t c a t c c g g g g t c a g c a c c g c g a a c g c a a a a g g g a a c a g g t c t a t c g g g t c a t c g a c t g t a a g t a g g c c c c a g t c g t g g

2700 TEASQSEVHEAEKLLGQARL g t t t c t g c g g a c t g g c t t t c t a c g t g t t c c g c t t c c t t t a g c a g c c c t t g c g c c c t g a g t c a a a g a c g c c t g a c c g a a a g a t g c a c a a g g c g a a g g a a a t c g t c g g g a a c g c g g g a c t c a

2760 AQPLTFSERLQLLRVAVFSL g c t t g c g g c a g c g t g a a g c t t t c t c t g a g c t g t a a c a g c c t g a c c g c a a c a a a c g a g a g g c g a a c g c c g t c g c a c t t c g a a a g a g a c t c g a c a t t g t c g g a c t g g c g t t g t t t g c t c t c c

2820 ISVMRELNDPEEMRQREAGA a t c g a g a c c a t c c g c t c c a g a t t a t c c g g c t c c t c c a t g c g t t g c c t c t c g g c t c c t g c t t a g c t c t g g t a g g c g a g g t c t a a t a g g c c g a g g a g g t a c g c a a c g g a g a g c c g a g g a c g a

2880 GTKWAKHFEEIRWRHTYIDI c c g g t t t t c c a t g c c t t a t g g a a c t c c t c g a t c c g c c a g c g a t g g g t a t a a a t g t c g a t g g g c c a a a a g g t a c g g a a t a c c t t g a g g a g c t a g g c g g t c g c t a c c c a t a t t t a c a g c t a c

2940 VRLAQALSEVPEGTLLLWKL a c g c g c a a g g c t t g g g c t a g c g a c t c g a c c g g t t c g c c g g t c a g c a a c a a c c a t t t c a a c t g c g c g t t c c g a a c c c g a t c g c t g a g c t g g c c a a g c g g c c a g t c g t t g t t g g t a a a g t t g

3000 PTEGKPPNIEEALVANLTIN g g g g t c t c a c c c t t g g g c g g g t t a a t c t c c t c g g c c a g c a c c g c g t t g a g c g t g a t a t t c c c c c a g a g t g g g a a c c c g c c c a a t t a g a g g a g c c g g t c g t g g c g c a a c t c g c a c t a t a a g

3060 GQKLTIRGSRM c c c t g t t t t a g c g t g a t g c g c c c a c t g c g c a t a g a a a t t g c a t c a a c g c a t a t a g c g c t a g g g a c a a a a t c g c a c t a c g c g g g t g a c g c g t a t c t t t a a c g t a g t t g c g t a t a t c g c g a t

3120 g c a g c a c g c c a t a g t g a c t g g c g a t g c t g t c g g a a t g g a c g a t a t c t a g a c t t a t a t a g a c g t c g t g c g g t a t c a c t g a c c g c t a c g a c a g c c t t a c c t g c t a t a g a t c t g a a t a t a t c t

3180 c a c t a a t a t a g a c a a t a g t t t a t a c t g c t a t c t a t a c a a g t a t a g a c a t t a t c t a a t c a t g t g a t t a t a t c t g t t a t c a a a t a t g a c g a t a g a t a t g t t c a t a t c t g t a a t a g a t t a g t a

3240 g g c a g a c a a a a c t c t a g c c a c t t t t c g t a t t g a c t c c g a a g a a t g g g a g t c t t t t a a a a a c c g t c t g t t t t g a g a t c g g t g a a a a g c a t a a c t g a g g c t t c t t a c c c t c a g a a a a t t t t t

3300 c c t t g c t a g t t c t g a a a g t t c c a a c g c c t c a g c a c t g t t a a c a g a a t t t g t t c g t t g g t a g g a a c g a t c a a g a c t t t c a a g g t t g c g g a g t c g t g a c a a t t g t c t t a a a c a a g c a a c c a t

3360 t t t g g c a g g t a a c a g g t t t a a t a c t c c c a c t t c t c a c a c t c c c a c c c a t c t a g a c a c a t c a a a c c g t c c a t t g t c c a a a t t a t g a g g g t g a a g a g t g t g a g g g t g g g t a g a t c t g t g t a g

3420 c c t c g a a c a g c g t a t a g a c a a t a t t g a a c a a c g t c t a g a t a a a g t c a c a a c t a a t a a t c t g g a g c t t g t c g c a t a t c t g t t a t a a c t t g t t g c a g a t c t a t t t c a g t g t t g a t t a t t a g a

3480 a g a c a a t a t a g a t g a a t t t a t a g a c a a g c g t a t a g a a g a t a a t c t a g c a a c a c g t c t a g a t c t g t t a t a t c t a c t t a a a t a t c t g t t c g c a t a t c t t c t a t t a g a t c g t t g t g c a g a t c t

3540 c a a a c t t c a a t c g c a a c t g g a g g a a c t g c g g g g a a a a t c g a a a g c c c g g t a g t t c a g g c a g t t t g a a g t t a g c g t t g a c c t c c t t g a c g c c c c t t t t a g c t t t c g g g c c a t c a a g t c c g t

3600 g a a g g a c a a g c t a c c g g g c a a g a c a g a a a g a a t a t a g a c a a t a g t a t a g a c a a t c t a g a c c t t c c t g t t c g a t g g c c c g t t c t g t c t t t c t t a t a t c t g t t a t c a t a t c t g t t a g a t c t g

3660 a a a t t g g a g g c a a c c c g c g a t c g c a c c c t c a a t a a g c t a a a a a t g g g t a g g c a g t c a g c c t t t a a c c t c c g t t g g g c g c t a g c g t g g g a g t t a t t c g a t t t t t a c c c a t c c g t c a g t c g g

3720 g c c g g g a a a g c c a t c g a c g c g t t t a t c a a a g a g t t g c t t t c t t c a g g a g a c a a c a t a a g c c g g c c c t t t c g g t a g c t g c g c a a a t a g t t t c t c a a c g a a a g a a g t c c t c t g t t g t a t t c g

3780 t g a a g t t a t c a a a a t t c t g t c c t t a c g t c g a a a g c c t g a t t t t a c c g t g c a a c g a t t g a t a c t t c a a t a g t t t t a a g a c a g g a a t g c a g c t t t c g g a c t a a a a t g g c a c g t t g c t a a c t a

3840 a a g c t t g g c t a a a c t a g c a c t g g c t t t c a a c a g a a a g c a t a c g a a g a a t c a a t a g a t a t a t t c g a a c c g a t t t g a t c g t g a c c g a a a g t t g t c t t t c g t a t g c t t c t t a g t t a t c t a t a t

3900 g c c a c c a a t t c c a c a a a a t g c a g a t a a c g t g t a g a g t a t t g g a a t g c t t a a t c t g t a a g g c g g t g g t t a a g g t g t t t t a c g t c t a t t g c a c a t c t c a t a a c c t t a c g a a t t a g a c a t t c c

3960 MKVNGNGRAKILTSDELRR g t t a t g a a g g t t a a c g g c a a c g g a c g a g c c a a a a t a c t c a c c t c c g a c g a a c t c a g g c g a c a a t a c t t c c a a t t g c c g t t g c c t g c t c g g t t t t a t g a g t g g a g g c t g c t t g a g t c c g c t

4020 LFSDGFTTPRDRVLFGICLF c t g t t t a g c g a c g g a t t c a c c a c a c c g c g c g a t c g c g t t t t g t t t g g c a t c t g t c t a t t c g a c a a a t c g c t g c c t a a g t g g t g t g g c g c g c t a g c g c a a a a c a a a c c g t a g a c a g a t a a g

4080 TGCRVSEALALQTTDIKGET a c c g g t t g c c g c g t t a g t g a a g c t c t a g c a c t c c a a a c a a c g g a c a t t a a a g g c g a a a c a t g g c c a a c g g c g c a a t c a c t t c g a g a t c g t g a g g t t t g t t g c c t g t a a t t t c c g c t t t g t

4140 LTFRKSTTKGKLKTRVVDIQ c t a a c c t t t a g g a a g t c t a c c a c c a a a g g g a a a c t c a a a a c c c g c g t g g t t g a c a t c c a g g a t t g g a a a t c c t t c a g a t g g t g g t t t c c c t t t g a g t t t t g g g c g c a c c a a c t g t a g g t c

4200 PGLAALMADYHPKPGTLFPG c c a g g a c t a g c c g c a c t c a t g g c t g a c t a t c a c c c c a a a c c g g g a a c c c t g t t c c c t g g c g g t c c t g a t c g g c g t g a g t a c c g a c t g a t a g t g g g g t t t g g c c c t t g g g a c a a g g g a c c g

4260 MRGVSDRLTRYAADKILRDA a t g a g g g g a g t c a g c g a t a g g c t c a c g c g a t a c g c g g c g g a t a a a a t c t t g c g c g a t g c a t a c t c c c c t c a g t c g c t a t c c g a g t g c g c t a t g c g c c g c c t a t t t t a g a a c g c g c t a c g t

4320 AKRIGLEGISTHSFRRTALN g c c a a a a g a a t c g g g c t a g a a g g c a t c a g t a c c c a c a g t t t c c g c c g t a c t g c c c t c a a c c g g t t t t c t t a g c c c g a t c t t c c g t a g t c a t g g g t g t c a a a g g c g g c a t g a c g g g a g t t g

4380 QMSSAGIPLRHIQEISGHND c a a a t g t c t a g c g c c g g t a t c c c g t t g c g a c a c a t t c a a g a g a t a t c c g g t c a c a a t g a c g t t t a c a g a t c g c g g c c a t a g g g c a a c g c t g t g t a a g t t c t c t a t a g g c c a g t g t t a c t g

4440 LGTLQRYLEVTPEQRRKAVS c t t g g c a c a c t g c a a c g c t a t c t t g a a g t t a c a c c c g a a c a g c g a c g c a a a g c t g t a t c c g a a c c g t g t g a c g t t g c g a t a g a a c t t c a a t g t g g g c t t g t c g c t g c g t t t c g a c a t a g g

4500 VIGF* g t g a t t g g c t t c t a a t g t a c g c c a a c g c t g t t t a g a c c c c t a t g g g t g c t a a a a a a a g a c c a c t a a c c g a a g a t t a c a t g c g g t t g c g a c a a a t c t g g g g a t a c c c a c g a t t t t t t t c t g

4560 g c a g c c t a a a c a c a c g c t c t a c a c t t g a g g a t a c t t t t a a a g t a t c c a t c g g t t c t a g a a c g t c g g a t t t g t g t g c g a g a t g t g a a c t c c t a t g a a a a t t t c a t a g g t a g c c a a g a t c t t

BspEI 4620 c t c t g c a c a c g t t c c g g a c t t t g g a a a c g t t a t a c c t t t c c c t g t g t t g c a g a a t g c t g c g a g a c g t g t g c a a g g c c t g a a a c c t t t g c a a t a t g g a a a g g g a c a c a a c g t c t t a c g a c g

4680 a a t a t t t c t t c g a c a a g t t a a c t t g t g a c t g g t t t a a t a t t t t c t c a a a t t g c c c c a a a a t t a t a a a g a a g c t g t t c a a t t g a a c a c t g a c c a a a t t a t a a a a g a g t t t a a c g g g g t t t t

4740 c a a c a c g c c t a a a t c c t t a g a c g t t t c t g t g g a a a c c t a t t a g g t t t t t a t c g c c g t t g t g t t g t g c g g a t t t a g g a a t c t g c a a a g a c a c c t t t g g a t a a t c c a a a a a t a g c g g c a a c a

4800 t t t a g t g g t a a a c c c a a a g g g t t t g t a t a t t c t t g t a t g a a g t t c g a c t c t g a g g g t t a a a a a t c a c c a t t t g g g t t t c c c a a a c a t a t a a g a a c a t a c t t c a a g c t g a g a c t c c c a a t t

4860 g a a g a a t g g c t c g c c g a a t t t t t t a c a a g t g g a a a c c g a t t a a a g g t t a a g g g t c a a t c g c t t c t t a c c g a g c g g c t t a a a a a a t g t t c a c c t t t g g c t a a t t t c c a a t t c c c a g t t a g c

4920 g g a c g a t g a a t a t t t t c t a a t t g t g a c c t t c t c c a t c t a a t a a g c t t t c t t t g g g g t t a a c c t g c t a c t t a t a a a a g a t t a a c a c t g g a a g a g g t a g a t t a t t c g a a a g a a a c c c c a a t t

ScaI 4980 g g t c g a a g a a a g t a c t a c g c a t g a t c t g c a t a c g a t c t c t a t t g c c a a a a a g c c g c g a c c c c a g c t t c t t t c a t g a t g c g t a c t a g a c g t a t g c t a g a g a t a a c g g t t t t t c g g c g c t g g

SpeI 5040 c t a t a g g c t c t c g g t c a t g c t g c a c t a g t t c g t g t c g a t c a c t a t a c t g g t t g c c g c a g c g a t a t c c g a g a g c c a g t a c g a c g t g a t c a a g c a c a g c t a g t g a t a t g a c c a a c g g c g t c g

BglI 5100 *IEGSHCG a t t t c a c g c t a a a a a a a a a t t c t t a a a a a t g t c c t t c a t a t c t c g c c a g a g t g g c a a c c t t a a a g t g c g a t t t t t t t t t a a g a a t t t t t a c a g g a a g t a t a g a g c g g t c t c a c c g t t g g a

5160 IVFRNGVRGARNESFHCQSL a t t a c a a a a c g g t t g c c t a c c c g a c c g g c t c g a t t t t c g c t g a a g t g g c a c t g t g a c a g t t a a t g t t t t g c c a a c g g a t g g g c t g g c c g a g c t a a a a g c g a c t t c a c c g t g a c a c t g t c a

5220 KFHYKRRAASMTTLTFQERY t t g a a a t g g t a c t t c c g c c g t g c t g c t g a c a t c g t t g t t a g g g t g a a t t g t t c g c g g t a g a a c t t t a c c a t g a a g g c g g c a c g a c g a c t g t a g c a a c a a t c c c a c t t a a c a a g c g c c a t c

5280 INCRNMFVKDGVKFLRGDFE a t g t t g c a c c g a t t c a t g a a c a c c t t g t c a c c c a c t t t g a a t a a t c g a c c g t c a a a t t c a t a c a a c g t g g c t a a g t a c t t g t g g a a c a g t g g g t g a a a c t t a t t a g c t g g c a g t t t a a g t

5340 TADIQYTNPQRKKAGPAIGD g t c g c g t c a a t t t g g t a a g t g t t g g g c t g t c t c t t t t t g g c t c c a g g g g c a a t g c c a t c a c a g c g c a g t t a a a c c a t t c a c a a c c c g a c a g a g a a a a a c c g a g g t c c c c g t t a c g g t a g t

5400 SFVVADGMVQYGIDTKTGTF g a a a a c a c a a c c g c g t c a c c c a t a a c t t g a t a a c c g a t a t c a g t t t t g g t t c c a g t g a a a c t t t t g t g t t g g c g c a g t g g g t a t t g a a c t a t t g g c t a t a g t c a a a a c c a a g g t c a c t t t

5460 AWFESADNNRAHRLQNYEIK g c c c a a a a t t c a g a c g c g t c a t t a t t c c g a g c g t g c c g g a g t t g a t t g t a c t c a a t t t t g c g g g t t t t a a g t c t g c g c a g t a a t a a g g c t c g c a c g g c c t c a a c t a a c a t g a g t t a a a a c

5520 AQCLQRRNMGLQKQHRRVTR g c t t g g c a a a g t t g a c g g c g a t t c a t g c c c a g c t g c t t t t g a t g t c g t c g c a c t g t g c g c c g a a c c g t t t c a a c t g c c g c t a a g t a c g g g t c g a c g a a a a c t a c a g c a g c g t g a c a c g c g

5580 KHIGLECSVAKQSTGYHIFS t t g t g a a t a c c c a a c t c a c a g c t g a c a g c t t t t t g a g a t g t a c c a t a g t g g a t g a a a c t t a a c a c t t a t g g g t t g a g t g t c g a c t g t c g a a a a a c t c t a c a t g g t a t c a c c t a c t t t g a a

5640 KSVFIRSSSIHLVCPVEGEP t t t g a g a c g a a t a t c c g c g a c g a a c t a a t g t g a a g t a c a c a a g g t a c t t c c c c c t c t g g c a a a c t c t g c t t a t a g g c g c t g c t t g a t t a c a c t t c a t g t g t t c c a t g a a g g g g g a g a c c g

5700 SKLSSQRTDKVLENPHPAGF g a t t t a a g a g a g g a t t g c c t t g t g t c c t t c a c t a g c t c g t t c g g g t g t g g c g c t c c a a a a c t a a a t t c t c t c c t a a c g g a a c a c a g g a a g t g a t c g a g c a a g c c c a c a c c g c g a g g t t t t

5760 LKRYEPKLQRNAAYRSKQQF a g t t t t c t g t a c t c t g g t t t a a g t t g t c t g t t g g c c g c a t a g c g g c t c t t t t g t t g a a a g t c a a a a g a c a t g a g a c c a a a t t c a a c a g a c a a c c g g c g t a t c g c c g a g a a a a c a a c t t t c

5820 SQTVIGTTLSRLDSLVQVLP c t t t g t g t g a c t a t g c c a g t g g t c a g t g a g c g t a a a t c g c t t a a c a c t t g g a c t a a a g g c g a a a c a c a c t g a t a c g g t c a c c a g t c a c t c g c a t t t a g c g a a t t g t g a a c c t g a t t t c c g

5880 VVAVDGWRKLNLNYCVQFMG a c t a c t g c a a c a t c a c c c c a t c t t t t t a a a t t t a g g t t g t a a c a a a c t t g a a a c a t a c c g t g a t g a c g t t g t a g t g g g g t a g a a a a a t t t a a a t c c a a c a t t g t t t g a a c t t t g t a t g g c

5940 GLYVTIMGAKIKYRRFAGIK c c c a a g t a g a c g g t t a t c a t t c c t g c t t t a a t t t t g t a g c g g c g g a a t g c t c c t a t t t t t g g g t t c a t c t g c c a a t a g t a a g g a c g a a a t t a a a a c a t c g c c g c c t t a c g a g g a t a a a a a

6000 KGDQLWRYVSKDSCDLFTQV t t t c c a t c c t g t a a c c a a c g g t a a a c a g a c t t a t c a c t a c a a t c t a a g a a c g t c t g t a c t a a a g g t a g g a c a t t g g t t g c c a t t t g t c t g a a t a g t g a t g t t a g a t t c t t g c a g a c a t g a

6060 VPLPLTLHGSGDKDLARCLY a c a g g c a a t g g c a a t g t t a a a t g a c c a g a c c c a t c c t t a t c a a g c g c t c g a c a c a a a t a c t g t c c g t t a c c g t t a c a a t t t a c t g g t c t g g g t a g g a a t a g t t c g c g a g c t g t g t t t a t g

6120 WLRACPERGIRTHVRVTYTG c a c a a c c g c g c a c a a g g t t c t c g a c c a a t g c g a g t g t g t a c c c t g a c c g t g t a a g t g c c a g t g t t g g c g c g t g t t c c a a g a g c t g g t t a c g c t c a c a c a t g g g a c t g g c a c a t t c a c g g t

6180 LIIETQLERTLLTLSVNTNL a g a a t t a t t t c a g t t t g t a g t t c c c t t g t a a g c a g g g t t a g t g a t a c a t t t g t a t t t a a g t c t t a a t a a a g t c a a a c a t c a a g g g a a c a t t c g t c c c a a t c a c t a t g t a a a c a t a a a t t c

6240 SEPSIM c t t t c t g g g c t g a t c a t t t g g a a a t g t c t c a g t c c a g t a c c t a t t g a a t g t t a t t t g c t t g a a a g a c c c g a c t a g t a a a c c t t t a c a g a g t c a g g t c a t g g a t a a c t t a c a a t a a a c g a a

6300 a a c c t g a a g c t a a a t a a a a c t t g t t a a c t a c a c c c a t t a a t t g a t a a a t t c a a a g c a c g t t t g g a c t t c g a t t t a t t t t g a a c a a t t g a t g t g g g t a a t t a a c t a t t t a a g t t t c g t g c a

6360 t t t t t c t g t t t g g t g t t t g g t g t g g t a a c a a t t c t g t g t a t g t g t g t t t t a t t t a g c t t c a a a a a g a c a a a c c a c a a a c c a c a c c a t t g t t a a g a c a c a t a c a c a c a a a a t a a a t c g a a g

6420 g g t t a a g t a g c a t a a c a a c c t c c a a g c a c t g a a c t t t t t t t a a t a g g t a a t t t a a a c t t t c c a a t t c a t c g t a t t g t t g g a g g t t c g t g a c t t g a a a a a a a t t a t c c a t t a a a t t t g a a a

6480 g c c t a t c g g c a a a a t t t t c a a t c a a t t g t a c g c c a a a g t g t t g c a t g a t c a a c g t t t g a c c g g a t a g c c g t t t t a a a a g t t a g t t a a c a t g c g g t t t c a c a a c g t a c t a g t t g c a a a c t g

6540 t t a t t t t t g t a t t t a c t a a a t a c t g a a t t t c g c c g t g a c g c t t t t t a c a g a t g g a a a t t c a a t a a a a a c a t a a a t g a t t t a t g a c t t a a a g c g g c a c t g c g a a a a a t g t c t a c c t t t a a g

6600 a c g g c a a a a t g t t t t t t g c t a a c t t t g c t a t g t a a a a c a a g a a a c t t g g c a c t c g g t t a t t g c c g t t t t a c a a a a a a c g a t t g a a a c g a t a c a t t t t g t t c t t t g a a c c g t g a g c c a a t a

6660 t a c t a a a t a a a c t g g t a a a a a a t a a c c a t t a g a a c c a a a a a g a a c g a a a a c c a g t a c a c c a t g a t t t a t t t g a c c a t t t t t t a t t g g t a a t c t t g g t t t t t c t t g c t t t t g g t c a t g t g g

6720 c t t g c c a g t t t t c a a g c t t t t g c t a t g a c g a c t c t a a t a a t c g g g t t t a a c a c c a t t c c g g a a c g g t c a a a a g t t c g a a a a c g a t a c t g c t g a g a t t a t t a g c c c a a a t t g t g g t a a g g c

6780 c t t t g a g a a a a t t a t c c t t g t a c a g c a a g t a a c a g t c a a t g c t a a a c g c a c c g c t a c a a a g a a a c t c t t t t a a t a g g a a c a t g t c g t t c a t t g t c a g t t a c g a t t t g c g t g g c g a t g t t t

6840 t c c t t a a g t t t t t c c a g t a g c g a t t t a c c t t c t t g g t a a c t g c c c g c c t t g a t a g c c c a g a g g a a t t c a a a a a g g t c a t c g c t a a a t g g a a g a a c c a t t g a c g g g c g g a a c t a t c g g g t c

6900 a a t g t c t t t a a t c a c t g t a a c t t t c t g a a a a a c g c c t g c g t t c c a g a c a g g c t t t t g c c a t t a c a g a a a t t a g t g a c a t t g a a a g a c t t t t t g c g g a c g c a a g g t c t g t c c g a a a a c g g t

6960 c t t t t g c t a g g g t t t c a t c t c t t g g a t c t g g t a g g g a t g a a a c g g g c t g t a a t g c t t g t t g a a a a c g a t c c c a a a g t a g a g a a c c t a g a c c a t c c c t a c t t t g c c c g a c a t t a c g a a c a a

7020 c t g a g g t c g g t t g a c g c c g t t g t g g a c g t g g g t g a a a a c t g g t t a c a g a c t g a a c c g g g g g a c t c c a g c c a a c t g c g g c a a c a c c t g c a c c c a c t t t t g a c c a a t g t c t g a c t t g g c c c c

7080 c a t a a c c a t t a t g t a a c t g c t t t a c a t c t t g g t a a c t g a c a c g g c a t a t c a t c c a c c a t g g t a t t g g t a a t a c a t t g a c g a a a t g t a g a a c c a t t g a c t g t g c c g t a t a g t a g g t g g t a c

7140 *PSP c a g c g a t a t t t c c c c g a g c t t t a a c c a c t c c a c a a g g g c a a g g t c t t t t a a g g a c t t g g c g t c g c t a t a a a g g g g c t c g a a a t t g g t g a g g t g t t c c c g t t c c a g a a a a t t c c t g a a c c g

7200 TALQLSVWAYCGLCKRVGGE g t g g c t a a c t g c a a a c t t a c c c a g g c g t a a c a t c c t a a a c a c t t a c g g a c a c c g c c t t c a c a c c g a t t g a c g t t t g a a t g g g t c c g c a t t g t a g g a t t t g t g a a t g c c t g t g g c g g a a g t

Bsu36I 7260 GEIGLTKVNDDQTLGIVFRK c c c t c g a t a c c t a a g g t c t t g a c a t t a t c a t c t t g a g t c a g c c c a a t a a c a a a a c g c t t g g g g a g c t a t g g a t t c c a g a a c t g t a a t a g t a g a a c t c a g t c g g g t t a t t g t t t t g c g a a c

7320 PKRGRRAHKILWETAIEVED g g c t t g c g g c c g c g c c t g g c g t g t t t g a t g a g c c a t t c g g t t g c t a t c t c g a c t t c a t c t c c g a a c g c c g g c g c g g a c c g c a c a a a c t a c t c g g t a a g c c a a c g a t a g a g c t g a a g t a g a

7380 RLLPLEEAILVSEKGALAKD c t c a g c a g t g g c a g t t c t t c a g c a a t t a a a a c g c t t t c t t t t c c t g c t a g t g c c t t a t c c g a g t c g t c a c c g t c a a g a a g t c g t t a a t t t t g c g a a a g a a a a g g a c g a t c a c g g a a t a g g

7440 GSEGRLEIRRQLEELDAAMG c c a g a c t c a c c c c g t a g c t c a a t c c g g c g c t g c a a t t c c t c c a g g t c a g c a g c c a t g c c c g g t c t g a g t g g g g c a t c g a g t t a g g c c g c g a c g t t a a g g a g g t c c a g t c g t c g g t a c g g g

7500 SQIAEFDGRRGIVNLGTWED g a c t g t a t a g c c t c a a a g t c a c c a c g g c g g c c a a t g a c a t t t a a c c c c g t c c a c t c g t c c c t g a c a t a t c g g a g t t t c a g t g g t g c c g c c g g t t a c t g t a a a t t g g g g c a g g t g a g c a g g

7560 PAADADYVTVEGGVQYALWQ g g t g c a g c g t c a g c g t c a t a g a c t g t c a c c t c a c c c c c g a c t t g a t a a g c a a g c c a t t g g c c a c g t c g c a g t c g c a g t a t c t g a c a g t g g a g t g g g g g c t g a a c t a t t c g t t c g g t a a c c

7620 AITSKGTGTDGVILCHKGSL g c t a t g g t g c t t t t g c c a g t t c c c g t a t c c c c a a c t a t t a a a c a g t g c t t a c c a g a c a g a c g a t a c c a c g a a a a c g g t c a a g g g c a t a g g g g t t g a t a a t t t g t c a c g a a t g g t c t g t c t

7680 AQMLDTIIGEPEVALTVATT g c t t g c a t c a a g t c g g t g a t g a t t c c c t c t g g t t c g a c c g c a a g g g t g a c g g c g g t a g t g c g a a c g t a g t t c a g c c a c t a c t a a g g g a g a c c a a g c t g g c g t t c c c a c t g c c g c c a t c a c

7740 DIIAAGYTGAYPLQDYVKVL t c a a t g a t a g c c g c g c c g t a a g t g c c a g c a t a g g g c a a t t g g t c g t a a a c t t t g a c c a a g a g t t a c t a t c g g c g c g g c a t t c a c g g t c g t a t c c c g t t a a c c a g c a t t t g a a a c t g g t t c

7800 NYVSQRCWKVVTLATQLAYS t t g t a t a c a g a c t g t c t a c a c c a c t t c a c c a c t g t t a a c g c t g t t t g c a a a g c g t a a g a c a a c a t a t g t c t g a c a g a t g t g g t g a a g t g g t g a c a a t t g c g a c a a a c g t t t c g c a t t c t g

7860 TADFLFISASFTLRGLGWLL g t g g c a t c a a a t a a a a a t a t g c t g g c a c t a a a a g t t a a t c g c c c c a a t c c c c a c a g t a a a c a c c g t a g t t t a t t t t t a t a c g a c c g t g a t t t t c a a t t a g c g g g g t t a g g g g t g t c a t t t

7920 FRGLQQRSPLHMEIWDNAM a a c c t g c c t a g c t g t t g a c g a c t a g g c a a g t g c a t t t c a a t c c a g t c a t t t g c c a t a a a t t t g g a c g g a t c g a c a a c t g c t g a t c c g t t c a c g t a a a g t t a g g t c a g t a a a c g g t a t t t a

7980 c a c c c c g t c t t t a a a g c c t t g c a g t t g a g c g c g a c a g g t a t t t a a c t g t g c t t g t a a c t c g t g g g g c a g a a a t t t c g g a a c g t c a a c t c g c g c t g t c c a t a a a t t g a c a c g a a c a t t g a g

8040 t g t t t g c t g g t t t t g a t a c c a c a g a c t g a c g g c g g c g g c c g c c a g t c c t a a a a a t a g a a a a c a a a c g a c c a a a a c t a t g g t g t c t g a c t g c c g c c g c c g g c g g t c a g g a t t t t t a t c t t t

8100 c t g g c g a t c g c t c a t t a t t g a c t t a c t c c c t g t t g a t t a g c g t g g t a g t g a g t c a t a g c c g a c c g c t a g c g a g t a a t a a c t g a a t g a g g g a c a a c t a a t c g c a c c a t c a c t c a g t a t c g g

8160 g c a t t g a c c g c t t c t t g g g c t t g g g g t g t t c t g c c a a g a t t g g g t t t t g t a g g g t c a t c g c g t a a c t g g c g a a g a a c c c g a a c c c c a c a a g a c g g t t c t a a c c c a a a a c a t c c c a g t a g c

8220 t t g g c t a c g a c t a a g g a c g c t t g t t c g g c t a t c g c t t g c g g g a c a c c a a c t t t a g t t a a c a a c c g a t g c t g a t t c c t g c g a a c a a g c c g a t a g c g a a c g c c c t g t g g t t g a a a t c a a t t g

8280 t c t g t c a a g g a t a c t t g g t a a a g t c g c t c g t t c a t t a g c c g a t t c t c c g g t a c a t a a a a c a g a c a g t t c c t a t g a a c c a t t t c a g c g a g c a a g t a a t c g g c t a a g a g g c c a t g t a t t t t g

8340 t g t t g c t g g c a g t c c c t t c a t t g g c g a c g a g t t c t t c a g c c g g a g t a t c a g c g a t a a t g t a c a a c g a c c g t c a g g g a a g t a a c c g c t g c t c a a g a a g t c g g c c t c a t a g t c g c t a t t a c a

8400 c a g c c c a g c c g g t g a c a t t a t t a t t a a t a a t g t t t t g t t c g g c a a t t g c a c c c a a g c c a g g t c g g g t c g g c c a c t g t a a t a a t a a t t a t t a c a a a a c a a g c c g t t a a c g t g g g t t c g g t c

8460 g a c g c g c c g t t t c a a a c t c a g a g a t g a c t t g c t g c t c t t t c t c g g t g a g t g g t c t a t c t g c t g c g c g g c a a a g t t t g a g t c t c t a c t g a a c g a c g a g a a a g a g c c a c t c a c c a g a t a g a c

8520 t c a t g a t a a t t a t g t c c t t c a t t a t g t a g g c g a t t c c a g t g g g t g t t t a c g a g g c a g t c c a g t a c t a t t a a t a c a g g a a g t a a t a c a t c c g c t a a g g t c a c c c a c a a a t g c t c c g t c a g g

8580 a c a g g a a t c a g t g c g a t t c a c c t t t a a g g t g a a t c g t c a t c a a a a a a t c a c t c g g t a g c a t g t c c t t a g t c a c g c t a a g t g g a a a t t c c a c t t a g c a g t a g t t t t t t a g t g a g c c a t c g t

8640 a c g a c c c g a a c c g a c c a g g a t t g a t t t c c c g g t t c t c a g t t c g c a g g c t t t t g a g c g c g t t g c t g g g c t t g g c t g g t c c t a a c t a a a g g g c c a a g a g t c a a g c g t c c g a a a a c t c g c g c a

8700 c a c c t t g a c c a t t g g g t a a c t g c c a t c a g c c g a t a a g c t a a a c g g g c t g t a t a g c g g t a a g t g g a a c t g g t a a c c c a t t g a c g g t a g t c g g c t a t t c g a t t t g c c c g a c a t a t c g c c a t t

8760 a g c a t c c c a c a c a g t c g g g c t g g c a t c a a c t t t g c a g g a a t a g c t c a c g t c a c t c a t c t c t c g t a g g g t g t g t c a g c c c g a c c g t a g t t g a a a c g t c c t t a t c g a g t g c a g t g a g t a g a g

8820 a c t c g c g c c t g g g t t g g a t g g c a g c g a a g g c a g a t t a c g a c g c a g t t t t t t a c t g g c a c t t g a g c g c g g a c c c a a c c t a c c g t c g c t t c c g t c t a a t g c t g c g t c a a a a a a t g a c c g t g a

8880 t t t a c c c g c a t t a a a a a c g g g t a c a g t g c c a t t g t t g a c g g t c t g t a c t t c g g t c a t a t a a a a t g g g c g t a a t t t t t g c c c a t g t c a c g g t a a c a a c t g c c a g a c a t g a a g c c a g t a t a t

8940 c t c g g t g t a c a c t t a a t a c a c t c t a t a c t a t t a c t g c c g a t t a g t a c a t t t g t c a a t c a c g a g c c a c a t g t g a a t t a t g t g a g a t a t g a t a a t g a c g g c t a a t c a t g t a a a c a g t t a g t g

DraIII 9000 t c t t t g c a c a a g g t g t a t g a t a t g g a c t c a g g a g t a c a c c a a a c g t c a t g c c a a c c a a t a a g a a a c g t g t t c c a c a t a c t a t a c c t g a g t c c t c a t g t g g t t t g c a g t a c g g t t g g t t a t

9060 a a g g g a g a a t a g c a g t c a c t c t a g a a g c t g a a a t t t a c c a a t g g a t t g c t a a c c g a g c g t t t c c c t c t t a t c g t c a g t g a g a t c t t c g a c t t t a a a t g g t t a c c t a a c g a t t g g c t c g c a

9120 c t g a g g a a g g a a g a c c g t t g g c t a a t c t t g c c g c t t t c t t a c t c a c a c g a g t t g t t a a a g g a c t c c t t c c t t c t g g c a a c c g a t t a g a a c g g c g a a a g a a t g a g t g t g c t c a a c a a t t t c

9180 a a c a a a t g g a a c a a g a a g c c a a g g a c a a c c a a g a c a a g c a g g g g g c a g c a t g a g c g a a g a t t g t t t a c c t t g t t c t t c g g t t c c t g t t g g t t c t g t t c g t c c c c c g t c g t a c t c g c t t c t

9240 c a g a c t a g c c a g a a t a g a a g c t g c g t t a g a c a g c c a a g t t g c a g t g a a t g c c g a c c t c c g g t c t g a t c g g t c t t a t c t t c g a c g c a a t c t g t c g g t t c a a c g t c a c t t a c g g c t g g a g g c

9300 c a c a t c g g t t a c a g a a c t c c g c g c a a c c g c a g a a g c a t t g t t g c a a a c a g t t c a a a t c c a g t g t a g c c a a t g t c t t g a g g c g c g t t g g c g t c t t c g t a a c a a c g t t t g t c a a g t t t a g g t

9360 t c a g c a g a a c t t t g a a a t t c t t a c c g c t a g g c a a t t a c a a a c c g a a g c a c g g c t t g a t g a a g t c g t c t t g a a a c t t t a a g a a t g g c g a t c c g t t a a t g t t t g g c t t c g t g c c g a a c t a c t

9420 g t a c c a a c g t a c c a c t a g c g c g g c a c t c g a c a g a a t t g g c g c g g t c t t a g a c t a c c t c g t c a t g g t t g c a t g g t g a t c g c g c c g t g a g c t g t c t t a a c c g c g c c a g a a t c t g a t g g a g c a

9480 t a g g c a g c a a a a c g g t t g a g g t g a g g g a t g a g c g a t g a c t a t c t a g a c g g a t a t c c c g c a a t c c g t c g t t t t g c c a a c t c c a c t c c c t a c t c g c t a c t g a t a g a t c t g c c t a t a g g g c g t

EcoO109I 9540 a g a g g c c c t t t c g t c t t c a a g a a t t c c c g t t t g a c t g g c g a t g c t g c t a c t g a a a c t a a c t c t c c g g g a a a g c a g a a g t t c t t a a g g g c a a a c t g a c c g c t a c g a c g a t g a c t t t g a t t g

9600 a a c t a c a t c g a c t a c g c a a t t a a c g c c c t c a g c t a a t t t t g c t t a g t c t a g g c c c g g a t g t t g a t g t a g c t g a t g c g t t a a t t g c g g g a g t c g a t t a a a a c g a a t c a g a t c c g g g c c t a c

9660 g g t a a g t g g t t t t c a g c t t a a g t g t t g g g t t c t a c t t a c t t c t c c g g g t c t t g c t c t a t c c c a t t c a c c a a a a g t c g a a t t c a c a a c c c a a g a t g a a t g a a g a g g c c c a g a a c g a g a t a g

9720 t a a a a a c a t t g g t t t a a c a a g g a g t a t t a g g c a a a t g c c a g t t a c t g t c g c t g c c t c t c g a t t t t t g t a a c c a a a t t g t t c c t c a t a a t c c g t t t a c g g t c a a t g a c a g c g a c g g a g a g c

9780 c t t g g g a a c c g c t g c g t t t g a c c a a t c a c c c g t c g a a c t g c g c g c t a a c t a t t c t c g a c c g a a c c c t t g g c g a c g c a a a c t g g t t a g t g g g c a g c t t g a c g c g c g a t t g a t a a g a g c t g g

BspDIClaISalI 9840 t g c a g g t c g a c g g t a t c g a t a a g c t t g a t a a c g c t t t t a c t t t t t c c t c t t g c c a t a g t t a c g t c c a g c t g c c a t a g c t a t t c g a a c t a t t g c g a a a a t g a a a a a g g a g a a c g g t a t c a a

9900 a a a a a a t t c t t t t t t a t c t g t t c t t c t c a c c c a c t a a a t t g c t t t t a t c c c t t c a c g a c c t t t t t t a a g a a a a a a t a g a c a a g a a g a g t g g g t g a t t t a a c g a a a a t a g g g a a g t g c t g g

9960 t t c g c g g t t t a c g g a a a g t a t g a g a t g a t t c c c c t a t a t t t c g g g g t a t t t a a t g a a t t g a a g c g c c a a a t g c c t t t c a t a c t c t a c t a a g g g g a t a t a a a g c c c c a t a a a t t a c t t a a c

10,020 g g t t a g t t t t c a c a c a t t c c c g c t t c t g c a c t c t t g c c g a c c t c c t t t a t t g a c a g g a g a c c a a t c a a a a g t g t g t a a g g g c g a a g a c g t g a g a a c g g c t g g a g g a a a t a a c t g t c c t c t

10,080 c g a g c t a a g a g c c a a t t t a c t t g a c g g a t a t t t a g t a g a g t a a t c a g c g c a a g a t g a a a a g c t c g a t t c t c g g t t a a a t g a a c t g c c t a t a a a t c a t c t c a t t a g t c g c g t t c t a c t t t t

10,140 a a t t g c a c a c c a c g t a a t t t g t a a c t t g t c t c a c a a t a t a t c a a g a a a t g g c a t a a g t t a t t a a c g t g t g g t g c a t t a a a c a t t g a a c a g a g t g t t a t a t a g t t c t t t a c c g t a t t c a a t

SwaI 10,200 t t c t t t g a a a g t t c t t a a c t t a c a t a t t t a a a t t t g t t a t t a c a t a a a g t t t t a a a c g t t a a g a a a c t t t c a a g a a t t g a a t g t a t a a a t t t a a a c a a t a a t g t a t t t c a a a a t t t g c a a

TspMIXmaISmaIBamHI 10,260 1 M GFP (S65T) M t t g t t a t c g a a t t c c t g c a g c c c g g g g g a t c c t c t a g a t t t a a g a a g g a g a t a t a c a t a t a a c a a t a g c t t a a g g a c g t c g g g c c c c c t a g g a g a t c t a a a t t c t t c c t c t a t a t g t a t a

10,320 5 10 15 20 SKGEELFTGVVPILVELDGD GFP (S65T) C A A C A G G G T T A A G A A C A A C T T A A T C T A C C GFP-R SKGEELFTGVVPILVELDGD g a g t a a a g g a g a a g a a c t t t t c a c t g g a g t t g t c c c a a t t c t t g t t g a a t t a g a t g g c g a c t c a t t t c c t c t t c t t g a a a a g t g a c c t c a a c a g g g t t a a g a a c a a c t t a a t c t a c c g c t

10,380 25 30 35 40 VNGQKFSVSGEGEGDATYGK GFP (S65T) VNGQKFSVSGEGEGDATYGK t g t t a a t g g g c a a a a a t t c t c t g t c a g t g g a g a g g g t g a a g g t g a t g c a a c a t a c g g a a a a c a a t t a c c c g t t t t t a a g a g a c a g t c a c c t c t c c c a c t t c c a c t a c g t t g t a t g c c t t t

10,440 45 50 55 60 LTLKFICTTGKLPVPWPTLV GFP (S65T) LTLKFICTTGKLPVPWPTLV a c t t a c c c t t a a a t t t a t t t g c a c t a c t g g g a a g c t a c c t g t t c c a t g g c c a a c a c t t g t t g a a t g g g a a t t t a a a t a a a c g t g a t g a c c c t t c g a t g g a c a a g g t a c c g g t t g t g a a c a

10,500 65 70 75 80 TTFAYGLQCFARYPDHMKQH GFP (S65T) TTFAYGLQCFARYPDHMKQH c a c t a c t t t c g c g t a t g g t c t t c a a t g c t t t g c g a g a t a c c c a g a t c a t a t g a a a c a g c a g t g a t g a a a g c g c a t a c c a g a a g t t a c g a a a c g c t c t a t g g g t c t a g t a t a c t t t g t c g t

10,560 85 90 95 100 DFFKSAMPEGYVQERTIFYK GFP (S65T) DFFKSAMPEGYVQERTIFYK t g a c t t t t t c a a g a g t g c c a t g c c c g a a g g t t a t g t a c a g g a a a g a a c t a t a t t t t a c a a a c t g a a a a a g t t c t c a c g g t a c g g g c t t c c a a t a c a t g t c c t t t c t t g a t a t a a a a t g t t

PmlI 10,620 105 110 115 120 DDGNYKTRAEVKFEGDTLVN GFP (S65T) DDGNYKTRAEVKFEGDTLVN a g a t g a c g g g a a c t a c a a g a c a c g t g c t g a a g t c a a g t t t g a a g g t g a t a c c c t t g t t a a t c t a c t g c c c t t g a t g t t c t g t g c a c g a c t t c a g t t c a a a c t t c c a c t a t g g g a a c a a t t

10,680 125 130 135 140 RIELKGIDFKEDGNILGHKM GFP (S65T) RIELKGIDFKEDGNILGHKM t a g a a t c g a g t t a a a a g g t a t t g a t t t t a a a g a a g a t g g a a a c a t t c t t g g a c a c a a a a t a t c t t a g c t c a a t t t t c c a t a a c t a a a a t t t c t t c t a c c t t t g t a a g a a c c t g t g t t t t a

PflMI 10,740 145 150 155 160 EYNYNSHNVYIMADKPKNGI GFP (S65T) EYNYNSHNVYIMADKPKNGI g g a a t a c a a c t a t a a c t c a c a t a a t g t a t a c a t c a t g g c a g a c a a a c c a a a g a a t g g a a t c c t t a t g t t g a t a t t g a g t g t a t t a c a t a t g t a g t a c c g t c t g t t t g g t t t c t t a c c t t a

10,800 165 170 175 180 KVNFKIRHNIKDGSVQLADH GFP (S65T) KVNFKIRHNIKDGSVQLADH c a a a g t t a a c t t c a a a a t t a g a c a c a a c a t t a a a g a t g g a a g c g t t c a a t t a g c a g a c c a g t t t c a a t t g a a g t t t t a a t c t g t g t t g t a a t t t c t a c c t t c g c a a g t t a a t c g t c t g g t

10,860 185 190 195 200 YQQNTPIGDGPVLLPDNHYL GFP (S65T) YQQNTPIGDGPVLLPDNHYL t t a t c a a c a a a a t a c t c c a a t t g g c g a t g g c c c t g t c c t t t t a c c a g a c a a c c a t t a c c t a a t a g t t g t t t t a t g a g g t t a a c c g c t a c c g g g a c a g g a a a a t g g t c t g t t g g t a a t g g a

10,920 205 210 215 220 STQSALSKDPNEKRDHMILL GFP (S65T) STQSALSKDPNEKRDHMILL g t c c a c a c a a t c t g c c c t t t c c a a a g a t c c c a a c g a a a a g a g a g a t c a c a t g a t c c t t c t c a g g t g t g t t a g a c g g g a a a g g t t t c t a g g g t t g c t t t t c t c t c t a g t g t a c t a g g a a g a

10,980 225 230 235 EFVTAAGITHGMDELYK * GFP (S65T) EFVTAAGITHGMDELYK* t g a g t t t g t a a c a g c t g c t g g g a t t a c a c a t g g c a t g g a t g a a c t a t a c a a a t a a g a a t t a c t c a a a c a t t g t c g a c g a c c c t a a t g t g t a c c g t a c c t a c t t g a t a t g t t t a t t c t t a a

3ʹ 5ʹ 11,002 c a g a c c t g c a g g c a t g c a a g c t g t c t g g a c g t c c g t a c g t t c g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.