Addgene pET Trx His6 LIC cloning vector (2Tc-T) Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pET Trx His6 LIC cloning vector (2Tc-T) Sequencing Result

Map View

800 600 400 200 End (870) BtgZI (851) BmtI (816) HaeII (813) NheI (812) AfeI (811) FspI - FspAI (783) BmrI (747) BspEI - BsaWI (661) BpuEI (648) EcoO109I (611) StyI (606) T7 Term (581 .. 599) BlpI (584) MspA1I (583) MluI - AflIII (519) KpnI (517) BanI - Acc65I (513) FseI (510) PflMI - DraIII (502) CsiI - SexAI * (498) AscI (491) PvuI - AsiSI (486) MflI * - BstYI - BamHI (476) AseI (457) XmnI (407) BaeGI - Bme1580I (385) ApaLI (381) BciVI (338) BtgI (322) BtsI - BtsαI (300) ClaI * - BspDI * (285) RsrII (203) TatI (151) HpaI - HincII (70) XbaI (29) AvaI - XhoI - PspXI - BsoBI - PaeR7I (22) PstI - SbfI (18) SfcI (14) NotI (6) Start (0) RBS TrxA 6xHis T7 terminator pET Trx His6 LIC cloning vector (2Tc-T) 870 bp

Sequence View

XbaIPaeR7IBsoBIPspXIXhoIAvaISbfIPstISfcINotIStart (0) 5ʹ 3ʹ 60 RBS LRGGQLVRTRSIIKNLKLFS t t a a g c g g c c g c c c t g c a g g a c t c g a g t t c t a g a a a t a a t t t t g t t t a a c t t t a a g a a g g a a t t c g c c g g c g g g a c g t c c t g a g c t c a a g a t c t t t a t t a a a a c a a a t t g a a a t t c t t c c

HincIIHpaI 120 1 5 MSDKII RBS TrxA IYNVEVELGARAARHAIFNN a g a t a t a g t t a a c c t c t a c t t c c a a t c c g g c t c t a g c g g c t c t a t g a g c g a t a a a a t t a t t c t a t a t c a a t t g g a g a t g a a g g t t a g g c c g a g a t c g c c g a g a t a c t c g c t a t t t t a a t a

TatI 180 10 15 20 25 HLTDDSFDTDVLKADGAILV TrxA VQSVVTKVRIYEFRVPRDED t c a c c t g a c t g a c g a c a g t t t t g a c a c g g a t g t a c t c a a a g c g g a c g g g g c g a t c c t c g t a g t g g a c t g a c t g c t g t c a a a a c t g t g c c t a c a t g a g t t t c g c c t g c c c c g c t a g g a g c a

RsrII 240 30 35 40 45 DFWAEWCGPCKMIAPILDEI TrxA IEPCLPATRAFHDGRNQIFD c g a t t t c t g g g c a g a g t g g t g c g g t c c g t g c a a a a t g a t c g c c c c g a t t c t g g a t g a a a t g c t a a a g a c c c g t c t c a c c a c g c c a g g c a c g t t t t a c t a g c g g g g c t a a g a c c t a c t t t a

BtsαIBtsIBspDI*ClaI* 300 50 55 60 65 ADEYQGKLTVAKLNIDQNPG TrxA SVFILAFQGNCFQVDILVRA c g c t g a c g a a t a t c a g g g c a a a c t g a c c g t t g c a a a a c t g a a c a t c g a t c a a a a c c c t g g g c g a c t g c t t a t a g t c c c g t t t g a c t g g c a a c g t t t t g a c t t g t a g c t a g t t t t g g g a c c

BciVIBtgI 360 70 75 80 85 TAPKYGIRGIPTLLLFKNGE TrxA SRRFIADTTDRSQQQEFVTF c a c t g c g c c g a a a t a t g g c a t c c g t g g t a t c c c g a c t c t g c t g c t g t t c a a a a a c g g t g a g t g a c g c g g c t t t a t a c c g t a g g c a c c a t a g g g c t g a g a c g a c g a c a a g t t t t t g c c a c t

XmnIApaLIBme1580IBaeGI 420 90 95 100 105 VAATKVGALSKGQLKEFLDA TrxA HRCGFHTCQRFTLQFLEEVS a g t g g c g g c a a c c a a a g t g g g t g c a c t g t c t a a a g g t c a g t t g a a a g a g t t c c t c g a c g c t c a c c g c c g t t g g t t t c a c c c a c g t g a c a g a t t t c c a g t c a a c t t t c t c a a g g a g c t g c g

BamHIBstYIMflI*AseI 480 NLA GSS 1 5 HHHHHH * TrxA 6xHis VQGATAVMVMVM t a a c c t g g c c g g c a g t a g c c a c c a t c a c c a t c a c c a t t a a t a a c c a a c t c c a t a a g g a t c a t t g g a c c g g c c g t c a t c g g t g g t a g t g g t a g t g g t a a t t a t t g g t t g a g g t a t t c c t a g

FseIAcc65IBanIKpnIAflIIIMluIAsiSIPvuIAscISexAI*CsiIDraIIIPflMI 540 c g c g a t c g c g g c g c g c c a c c t g g t g g c c g g c c g g t a c c a c g c g t g c g c g c t g a t c c g g c t g c g c t a g c g c c g c g c g g t g g a c c a c c g g c c g g c c a t g g t g c g c a c g c g c g a c t a g g c c g a

MspA1IBlpI 600 T7 terminator G G C G A C T C G T T A T T G A T C G T7 Term g c t a a c a a a g c c c g a a a g g a a g c t g a g t t g g c t g c t g c c a c c g c t g a g c a a t a a c t a g c a c g a t t g t t t c g g g c t t t c c t t c g a c t c a a c c g a c g a c g g t g g c g a c t c g t t a t t g a t c g t

BpuEIEcoO109IStyI 660 T7 terminator t a a c c c c t t g g g g c c t c t a a a c g g g t c t t g a g g g g t t t t t t g c t g a a a g g a g g a a c t a t a a t t g g g g a a c c c c g g a g a t t t g c c c a g a a c t c c c c a a a a a a c g a c t t t c c t c c t t g a t a t

BsaWIBspEI 720 t c c g g a c a t c c a c a g g a c g g g t g t g g t c g c c a t g a t c g c g t a g t c g a t a g t g g c t c c a a g a g g c c t g t a g g t g t c c t g c c c a c a c c a g c g g t a c t a g c g c a t c a g c t a t c a c c g a g g t t c

BmrI 780 t a g c g a a g c g a g c a g g a c t g g g c g g c g g c c a a a g c g g t c g g a c a g t g c t c c g a g a a c g g g a t c g c t t c g c t c g t c c t g a c c c g c c g c c g g t t t c g c c a g c c t g t c a c g a g g c t c t t g c c c

AfeINheIHaeIIBmtIFspAIFspI 840 t g c g c a t a g a a a t t g c a t c a a c g c a t a t a g c g c t a g c a g c a c g c c a t a g t g a c t g g c g a t a c g c g t a t c t t t a a c g t a g t t g c g t a t a t c g c g a t c g t c g t g c g g t a t c a c t g a c c g c t a

End (870) BtgZI 3ʹ 5ʹ 870 g c t g t c g g a a t g g a c g a c a t c c c g c a a g a g c g a c a g c c t t a c c t g c t g t a g g g c g t t c t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.