Addgene tandem PA-GFP-KanR Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: tandem PA-GFP-KanR Sequencing Result

Map View

800 600 400 200 End (928) BtgI - StyI - NcoI (732) BamHI (728) HincII (724) AccI (723) SalI (722) BsrGI (708) EGFP-C (652 .. 673) EXFP-R (305 .. 324) PfoI * (279) BtsI - BtsαI (212) Start (0) PA-GFP tandem PA-GFP-KanR 928 bp

Sequence View

Start (0) 5ʹ 3ʹ 60 1a V 5 10 15 20 SKGEELFTGVVPILVELDG PA-GFP GEQGRGAVHRGGAHPGRAGR VSKGEELFTGVVPILVELDG HALALLQEGPHHGDQDLQVA g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c

120 25 30 35 40 DVNGHKFSVSGEGEGDATYG PA-GFP RRKRPQVQRVRRGRGRCHLR DVNGHKFSVSGEGEGDATYG VYVAVLEAHGALALAIGGVA c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c

180 45 50 55 60 KLTLKFICTTGKLPVPWPTL PA-GFP QADPEVHLHHRQAARALAHP KLTLKFICTTGKLPVPWPTL LQGQLEDAGGALQGHGPGGE c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a

BtsαIBtsI 240 65 70 75 80 VTTFSYGVQCFSRYPDHMKQ PA-GFP RDHLQLRRAVLQPLPRPHEA VTTFSYGVQCFSRYPDHMKQ HGGEAVAHLAEAAVGVVHLL c g t g a c c a c c t t c a g c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c t g g t g g a a g t c g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t

PfoI* 300 85 90 95 100 HDFFKSAMPEGYVQERTIFF PA-GFP ARLLQVRHARRLRPGAHHLL HDFFKSAMPEGYVQERTIFF VVEELGGHGFAVDLLAGDEE g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a

360 105 110 115 120 KDDGNYKTRAEVKFEGDTLV PA-GFP C T G C T G C C G T T G A T G T T C T G EXFP-R QGRRQLQDPRRGEVRGRHPG KDDGNYKTRAEVKFEGDTLV LVVAVVLGAGLHLELAVGQH c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a

420 125 130 135 140 NRIELKGIDFKEDGNILGHK PA-GFP EPHRAEGHRLQGGRQHPGAQ NRIELKGIDFKEDGNILGHK VADLQLADVELLVAVDQPVL g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t

480 145 150 155 160 LEYNYNSHNVYIMADKQKNG PA-GFP AGVQLQQPQRLYHGRQAEER LEYNYNSHNVYIMADKQKNG QLVVVVAVVDIDHGVLLLVA g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c

540 165 170 175 180 IKANFKIRHNIEDGSVQLAD PA-GFP HQGQLQDPPQHRGRQRAARR IKANFKIRHNIEDGSVQLAD DLGVELDAVVDLVAAHLEGV c a t c a a g g c c a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a g t a g t t c c g g t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t

600 185 190 195 200 HYQQNTPIGDGPVLLPDNHY PA-GFP PLPAEHPHRRRPRAAARQPL HYQQNTPIGDGPVLLPDNHY VVLLVGGDAVAGHQQGVVVV c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t

660 205 210 215 220 LSHQSALSKDPNEKRDHMVL PA-GFP C A T G G T C C T EGFP-C PEPPVRPEQRPQREARSHGP LSHQSALSKDPNEKRDHMVL QAVLGGQAFVGVLLAIVHDQ c c t g a g c c a c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g g a c t c g g t g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a

BsrGI 720 225 230 235 LEFVTAAGITLGMDELYK GA PA-GFP G C T G G A G T T C G T G EGFP-C AGVRDRRRDHSRHGRAVQGC LEFVTAAGITLGMDELYKGA QLEHGGGPDSEAHVLQVLTS g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g g t g c c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c c c a c g

SalIAccIHincIIBamHINcoIStyIBtgI 780 (in frame with PA-GFP) VDGSMVSKGEELFTGVVPIL CRRIHGEQGRGAVHRGGAHP VDGSMVSKGEELFTGVVPIL DVSGHHALALLQEGPHHGDQ t g t c g a c g g a t c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t a c a g c t g c c t a g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a

840 (in frame with PA-GFP) VELDGDVNGHKFSVSGEGEG GRAGRRRKRPQVQRVRRGRG VELDGDVNGHKFSVSGEGEG DLQVAVYVAVLEAHGALALA g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c

900 (in frame with PA-GFP) DATYGKLTLKFICTTGKLPV RCHLRQADPEVHLHHRQAAR DATYGKLTLKFICTTGKLPV IGGVALQGQLEDAGGALQGH c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a

End (928) 3ʹ 5ʹ 928 (in frame with PA-GFP) PWPNPRDHL ALAQPS* PWPNPRDHL GPGVRTVVK g c c c t g g c c c a a c c c t c g t g a c c a c c t t c g g g a c c g g g t t g g g a g c a c t g g t g g a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.