Addgene pfwB - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pfwB


Map View

SbfI (7) PaqCI (5582) XcmI (5572) DrdI (5278) pBR322ori-F (5225 .. 5244) ScaI (4210) BmtI (3991) NheI (3987) AatII (3816) ZraI (3814) PflMI (3604) WPRE-R (3416 .. 3436) EcoRV (3347) SpeI (3315) pBluescriptSK (3309 .. 3325) BsrGI (3269) EGFP-C (3213 .. 3234) pBABE 3' (53 .. 73) AvrII (372) BclI * (423) PmlI (432) BspHI (499) EBV-rev (973 .. 992) SV40pA-R (1027 .. 1046) PsiI (1063) BsaBI * (1184) PvuI (1224) BspQI - SapI (1245) AclI (1458) AflII (1644) BglII (1831) FseI (1969) BbvCI (2167) KasI (2203) NarI (2204) SfoI (2205) PluTI (2207) EF1a-F (2391 .. 2411) BstBI (2508) SalI (2520) AccI (2521) EGFP-N (2605 .. 2626) EXFP-R (2866 .. 2885) pfwB 5904 bp

Sequence View

SbfI 60 SV40 promoter C C T T A C A C pBABE 3' c c c t g c a g g g c c t g a a a t a a c c t c t g a a a g a g g a a c t t g g t t a g g t a c c t g t g g a a t g t g g g g a c g t c c c g g a c t t t a t t g g a g a c t t t c t c c t t g a a c c a a t c c a t g g a c a c c t t a c a c

120 SV40 promoter A C A G T C A A T C C C A pBABE 3' t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c

180 SV40 promoter c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a

240 SV40 promoter SV40 ori a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g

300 SV40 promoter SV40 ori c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a

360 SV40 promoter SV40 ori a t t t a t a c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t a a a t a t g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g

AvrII 420 SV40 promoter SV40 ori t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t

PmlIBclI* 480 EM7 promoter *RCLIDAYYLV t c t g a t c a g c a c g t g t t g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c g a g a c t a g t c g t g c a c a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t g c

BspHI 540 EM7 promoter VLHPVLDHLGEVDRLLIQLQ a c a a g g t g a g g a a c t a a a t c a t g a a g a c c t t c a a c a t c t c t c a g c a g g a t c t g g a g c t g g t g t t c c a c t c c t t g a t t t a g t a c t t c t g g a a g t t g t a g a g a g t c g t c c t a g a c c t c g a c c

600 HLDGSLLDGHEILVVLVMDP t g g a g g t c g c c a c t g a g a a g a t c a c c a t g c t c t a t g a g g a c a a c a a g c a c c a t g t c g g g g a c c t c c a g c g g t g a c t c t t c t a g t g g t a c g a g a t a c t c c t g t t g t t c g t g g t a c a g c c c c

660 RGDPGLSPLDDRSDVNLGVN c g g c c a t c a g g a c c a a g a c t g g g g a g a t c a t c t c t g c t g t c c a c a t t g a g g c c t a c a t t g g c c g g t a g t c c t g g t t c t g a c c c c t c t a g t a g a g a c g a c a g g t g t a a c t c c g g a t g t a a c

720 APDSDTSFGNGNPRSHAVPL g c a g g g t c a c t g t c t g t g c t g a a g c c a t t g c c a t t g g g t c t g c t g t g a g c a a c g g g c a g a c g t c c c a g t g a c a g a c a c g a c t t c g g t a a c g g t a a c c c a g a c g a c a c t c g t t g c c c g t c t

780 LVKVGNHSDPVGVRILHVSG a g g a c t t t g a c a c c a t t g t g g c t g t c a g g c a c c c c t a c t c t g a t g a g g t g g a c a g a t c c a t c c t g a a a c t g t g g t a a c a c c g a c a g t c c g t g g g g a t g a g a c t a c t c c a c c t g t c t a g g t

840 DPHDAGTAHASLEDRVISRV t c a g g g t g g t c a g c c c c t g t g g c a t g t g c a g a g a g c t c a t c t c t g a c t a t g c t c c t g a c t a g t c c c a c c a g t c g g g g a c a c c g t a c a c g t c t c t c g a g t a g a g a c t g a t a c g a g g a c t g a

900 AKHENLHIALQDFGGNLFED g c t t t g t g c t c a t t g a g a t g a a t g g c a a g c t g g t c a a a a c c a c c a t t g a g g a a c t c a t c c c g a a a c a c g a g t a a c t c t a c t t a c c g t t c g a c c a g t t t t g g t g g t a a c t c c t t g a g t a g g

960 GELVGPVLGSNALPGSRASM c c c t c a a g t a c a c c a g g a a c t a a a c c t g a a t t c g c t a g a g g g c c g c t t c g a g c a g a c a t g g g g a g t t c a t g t g g t c c t t g a t t t g g a c t t a a g c g a t c t c c c g g c g a a g c t c g t c t g t a c

1020 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev ILYMSSNPCVVVLICHFFHK a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a

PsiI 1080 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R IQSIQSAIAKNTVMILQLLC a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t

1140 SV40 poly(A) signal TLLLLQM g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a

BsaBI* 1200 t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a a a a t c g a t a a g g a t c c g g g c t g g c g t a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t t t t a g c t a t t c c t a g g c c c g a c c g c a

BspQISapIPvuI 1260 a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g g t g g a g a a g a g c a t g c t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c c a c c t c t t c t c g t a c g

1320 EF-1 α promoter g t g a g g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t c a c t c c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a

1380 EF-1 α promoter g g g g g g a g g g g t c g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a c c c c c c t c c c c a g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t

1440 EF-1 α promoter a a g t g a t g t c g t g t a c t g g c t c c g c c c t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t t c a c t a c a g c a c a t g a c c g a g g c g g g a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c

AclI 1500 EF-1 α promoter EF-1 α intron A t g c a g t a g t c g c t g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g a c g t c a t c a g c g a c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c

1560 EF-1 α promoter EF-1 α intron A t g c c g t g t g t g g t t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a c g g c a c a c a c c a a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c

1620 EF-1 α promoter EF-1 α intron A *KWRAATRSEQDRAEPQFH a a t t a c t t c c a c c t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g t t a a t g a a g g t g g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c

AflII 1680 EF-1 α promoter EF-1 α intron A TPSNSAKRKLLGKAEHKLQP g t g g g a g a g t t c g a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c a c c c t c t c a a g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g

1740 EF-1 α promoter EF-1 α intron A RAQASPGGRAFRTAGERRDR c t g g c c t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c g a c c g g a c c c g c g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g

1800 EF-1 α promoter EF-1 α intron A QKRYTELWKFNKIVQQSAKK t g c t t t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t a c g a a a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a

BglII 1860 EF-1 α promoter EF-1 α intron A RALYDQLHPGLDACQYKPKQ c t g g c a a g a t a g t c t t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g a c c g t t c t a t c a g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c

1920 EF-1 α promoter EF-1 α intron A PRPRRRPGHTGACMNPSAPG g g c c g c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t c c g g c g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a

FseI 1980 EF-1 α promoter EF-1 α intron A ALAAVSFRVPTTELQGAQEP g c g a g c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t c g c t c g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a

2040 EF-1 α promoter EF-1 α intron A AQGRAATYRGARPPLAPGTP g c c t g g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c c g g a c c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g

2100 EF-1 α promoter EF-1 α intron A VLQTLPFIAAERGQQLSSLI a c c a g t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g t g g t c a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c

2160 EF-1 α promoter EF-1 α intron A SSAASPLAPPHTVWVFSFPR g a g g a c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t c t c c t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a

KasINarISfoIPluTIBbvCI 2220 EF-1 α promoter EF-1 α intron A ETRLRRKM t c c g t c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t a g g c a g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a

2280 EF-1 α promoter EF-1 α intron A c g a t t a g t t c t c g a g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g c t a a t c a a g a g c t c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g

2340 EF-1 α promoter EF-1 α intron A g a t g g a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t c t a c c t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a

2400 EF-1 α promoter EF-1 α intron A T C A A G C C T C A EF1a-F g t a a t t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a c a t t a a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t

2460 EF-1 α promoter EF-1 α intron A G A C A G T G G T T C EF1a-F g a c a g t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a g g a a t t c t c t a g a g a t c c t g t c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t c c t t a a g a g a t c t c t a g

BstBI 2520 c c t c g a c c t c g a g a t c c a t t g t g c t g g a t c t g c g a t c t a a g t a a g c t t c g a a t t c t g c a g g g a g c t g g a g c t c t a g g t a a c a c g a c c t a g a c g c t a g a t t c a t t c g a a g c t t a a g a c g t c

SalIAccI 2580 1 M 1a V 5 SKGEE EGFP 1 M 1a V 5 SKGEE EGFP Kozak sequence MVSKGEE t c g a c g g t a c c g c g g g c c c g g g a t c c a c c g g t c g c c a c c a t g g t g a g c a a g g g c g a g g a g a g c t g c c a t g g c g c c c g g g c c c t a g g t g g c c a g c g g t g g t a c c a c t c g t t c c c g c t c c t c

2640 10 15 20 25 LFTGVVPILVELDGDVNGHK EGFP 10 15 20 25 LFTGVVPILVELDGDVNGHK EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N LFTGVVPILVELDGDVNGHK c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c

2700 30 35 40 45 FSVSGEGEGDATYGKLTLKF EGFP 30 35 40 45 FSVSGEGEGDATYGKLTLKF EGFP FSVSGEGEGDATYGKLTLKF t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g

2760 50 55 60 65 ICTTGKLPVPWPTLVTTLTY EGFP 50 55 60 65 ICTTGKLPVPWPTLVTTLTY EGFP ICTTGKLPVPWPTLVTTLTY a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g

2820 70 75 80 85 GVQCFSRYPDHMKQHDFFKS EGFP 70 75 80 85 GVQCFSRYPDHMKQHDFFKS EGFP GVQCFSRYPDHMKQHDFFKS g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g

2880 90 95 100 105 AMPEGYVQERTIFFKDDGNY EGFP 90 95 100 105 AMPEGYVQERTIFFKDDGNY EGFP C T G C T G C C G T T G A T G EXFP-R AMPEGYVQERTIFFKDDGNY g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g

2940 110 115 120 125 KTRAEVKFEGDTLVNRIELK EGFP 110 115 120 125 KTRAEVKFEGDTLVNRIELK EGFP T T C T G EXFP-R KTRAEVKFEGDTLVNRIELK a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c

3000 130 135 140 145 GIDFKEDGNILGHKLEYNYN EGFP 130 135 140 145 GIDFKEDGNILGHKLEYNYN EGFP GIDFKEDGNILGHKLEYNYN g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g

3060 150 155 160 165 SHNVYIMADKQKNGIKVNFK EGFP 150 155 160 165 SHNVYIMADKQKNGIKVNFK EGFP SHNVYIMADKQKNGIKVNFK a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c

3120 170 175 180 185 IRHNIEDGSVQLADHYQQNT EGFP 170 175 180 185 IRHNIEDGSVQLADHYQQNT EGFP IRHNIEDGSVQLADHYQQNT a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g

3180 190 195 200 205 PIGDGPVLLPDNHYLSTQSA EGFP 190 195 200 205 PIGDGPVLLPDNHYLSTQSA EGFP PIGDGPVLLPDNHYLSTQSA c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g

3240 210 215 220 225 LSKDPNEKRDHMVLLEFVTA EGFP 210 215 220 225 LSKDPNEKRDHMVLLEFVTA EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C LSKDPNEKRDHMVLLEFVTA c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g

BsrGI 3300 230 235 AGITLGMDELYK* EGFP 230 235 AGITLGMDELYK * EGFP AGITLGMDELYK* g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a a g c g g c c g c t c c a c c g c g g t g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t t c g c c g g c g a g g t g g c g c c a c

EcoRVSpeI 3360 SK primer T C T A G A A C T A G T G G A T C pBluescriptSK g c g g c c g c t c t a g a a c t a g t g g a t c c c c c g g g c t g c a g g a a t t c g a t a t c a a g c t t a t c g c g c c g g c g a g a t c t t g a t c a c c t a g g g g g c c c g a c g t c c t t a a g c t a t a g t t c g a a t a g c

3420 WPRE A C A A C WPRE-R a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c

3480 WPRE G A G G A A A A T G C G A T A C WPRE-R MLLLP MPLYHAIAS c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g

3540 WPRE VWLSFSPPCINPGCCLFMRS RMAFIFSSLYKSWLLSLYEE g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a

3600 WPRE CGPLSGNVAWCALCLLTQPP LWPVVRQRGVVCTVFADATP t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t

PflMI 3660 WPRE LVGALPPPVSSFPGLSLSPS TGWGIATTCQLLSGTFAFPL c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g

3720 WPRE LLPRRNSSPPALPAAGQGLG PIATAELIAACLARCWTGAR c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g

3780 WPRE (in frame with Factor Xa site) *RGKRPQ CWALTIPWCCRGNHRPFLGC LLGTDNSVVLSGKSSSFPWL t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g

ZraIAatII 3840 WPRE (in frame with Factor Xa site) EGTNGGPNQAPRGEAVDR RG Factor Xa site SPVLPPGFCAGRPSATSLRP LACVATWILRGTSFCYVPSA t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g

3900 WPRE 1 EI Factor Xa site SIQRTFLPAACCRLCGLFRV LNPADLPSRGLLPALRPLPR t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g

3960 WPRE FAFALRRVGSPFGPPPRIDT LRLRPQTSRISLWAASPHRY t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g a t a c c a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c t a t g g

BmtINheI 4020 EF-1 α poly(A) signal VGPLLPKPELALSLIPATPL RRPTAP* g t c g g c c c a c t g c t c c c t a a a c c t g a g c t a g c a t t a t c c c t a a t a c c t g c c a c c c c a c t c c a g c c g g g t g a c g a g g g a t t t g g a c t c g a t c g t a a t a g g g a t t a t g g a c g g t g g g g t g a g

4080 EF-1 α poly(A) signal LISGGRTVSELFVSIGHLSL t t a a t c a g t g g t g g a a g a a c g g t c t c a g a a c t g t t t g t t t c a a t t g g c c a t t t a a g t t t a a a t t a g t c a c c a c c t t c t t g c c a g a g t c t t g a c a a a c a a a g t t a a c c g g t a a a t t c a a a t

4140 EF-1 α poly(A) signal VVKDWLMITMHRKTFRRKGE g t a g t a a a a g a c t g g t t a a t g a t a a c a a t g c a t c g t a a a a c c t t c a g a a g g a a a g g a g a a c a t c a t t t t c t g a c c a a t t a c t a t t g t t a c g t a g c a t t t t g g a a g t c t t c c t t t c c t c t t

4200 EF-1 α poly(A) signal CFVDHFGFLFCVWQF* t g t t t t g t g g a c c a c t t t g g t t t t c t t t t t t g c g t g t g g c a g t t t t a a g t t a t t a g t t t t a c a a a a c a c c t g g t g a a a c c a a a a g a a a a a a c g c a c a c c g t c a a a a t t c a a t a a t c a a a a

ScaI 4260 EF-1 α poly(A) signal t a a a a t c a g t a c t t t t t a a t g g a a a c a a c t t g a c c a a a a a t t t g t c a c a g a a t t t t g a g a a t t t t a g t c a t g a a a a a t t a c c t t t g t t g a a c t g g t t t t t a a a c a g t g t c t t a a a a c t c t

4320 EF-1 α poly(A) signal c c c a t t a a a a a a g t t a a a t g a g a a a c c t g t g t g t t c c t t t g g t c a a c a c c g a g a c a t t t a g g g t a a t t t t t t c a a t t t a c t c t t t g g a c a c a c a a g g a a a c c a g t t g t g g c t c t g t a a a t

4380 EF-1 α poly(A) signal g g t g a a a g a c a t c t a a t t c t g g t t t t a c g a a t c t g g a a a c t t c t t g a a a a t g t a a t t c t t c c a c t t t c t g t a g a t t a a g a c c a a a a t g c t t a g a c c t t t g a a g a a c t t t t a c a t t a a g a a

4440 EF-1 α poly(A) signal g a g t t a a c a c t t c t g g g t g g a g a a t a g g g t t g t t t t c c c c c c a c a t a a t t g g a a g g g g a a c t c a a t t g t g a a g a c c c a c c t c t t a t c c c a a c a a a a g g g g g g t g t a t t a a c c t t c c c c t t

4500 EF-1 α poly(A) signal g g a a t a t c a t t t a a a g c t a t g g g a g g g t t t c t t t g a t t a c a a c a c t g g a g a g a a a t g c a g c c t t a t a g t a a a t t t c g a t a c c c t c c c a a a g a a a c t a a t g t t g t g a c c t c t c t t t a c g t c

4560 EF-1 α poly(A) signal c a t g t t g c t g a t t g c c t g t c a c t a a a a c a g g c c a a a a a c t g a g t c c t t g g g t t g c a t a g a g t a c a a c g a c t a a c g g a c a g t g a t t t t g t c c g g t t t t t g a c t c a g g a a c c c a a c g t a t c t

4620 EF-1 α poly(A) signal a a g c t t c a t g t t g c t a a a c c a a t g t t a a g t g a a t c t t t g g a a a c a a a a t g t t t c c a a a t t t t c g a a g t a c a a c g a t t t g g t t a c a a t t c a c t t a g a a a c c t t t g t t t t a c a a a g g t t t a a

4680 a c t g g g a t g t g c a t g t t g a a a c g t g g g t t a a t t a a c t a g c c a t g a c c a a a a t c c c t t a a c t g a c c c t a c a c g t a c a a c t t t g c a c c c a a t t a a t t g a t c g g t a c t g g t t t t a g g g a a t t g

4740 ori g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c

4800 ori a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c

4860 ori t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t

4920 ori g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t

4980 ori a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t

5040 ori g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g

5100 ori a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t

5160 ori c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t

5220 ori a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g

DrdI 5280 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g

5340 ori g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c

5400 c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t a a t t a a a t t t t g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a t t a a t t t a a a a

5460 EM7 promoter T7 promoter t c a a a a g t a g t t g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c t c a a g t t t t c a t c a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g a g t

5520 T7 promoter c t a t a g g a g g g c c a t c a t g g c c a a g t t g a c c a g t g c t g t c c c a g t g c t c a c a g c c a g g g a g a t a t c c t c c c g g t a g t a c c g g t t c a a c t g g t c a c g a c a g g g t c a c g a g t g t c g g t c c c t

XcmI 5580 t g t g g c t g g a g c t g t t g a g t t c t g g a c t g a c a g g t t g g g g t t c t c c a g a g a t t t t g t g g a a c a c c g a c c t c g a c a a c t c a a g a c c t g a c t g t c c a a c c c c a a g a g g t c t c t a a a a c a c c t

PaqCI 5640 g g a t g a c t t t g c a g g t g t g g t c a g a g a t g a t g t c a c c c t g t t c a t c t c a g c a g t c c a g g a c c t a c t g a a a c g t c c a c a c c a g t c t c t a c t a c a g t g g g a c a a g t a g a g t c g t c a g g t c c t

5700 c c a g g t g g t g c c t g a c a a c a c c c t g g c t t g g g t g t g g g t g a g a g g a c t g g a t g a g c t g t a g g t c c a c c a c g g a c t g t t g t g g g a c c g a a c c c a c a c c c a c t c t c c t g a c c t a c t c g a c a t

5760 t g c t g a g t g g a g t g a g g t g g t c t c c a c c a a c t t c a g g g a t g c c a g t g g c c c t g c c a t g a c a c g a c t c a c c t c a c t c c a c c a g a g g t g g t t g a a g t c c c t a c g g t c a c c g g g a c g g t a c t g

5820 a g a g a t t g g a g a g c a g c c c t g g g g g a g a g a g t t t g c c c t g a g a g a c c c a g c a g g c a a c t g t c t c t a a c c t c t c g t c g g g a c c c c c t c t c t c a a a c g g g a c t c t c t g g g t c g t c c g t t g a c

5880 t g t g c a c t t t g t g g c a g a g g a g c a g g a c t g a g g a t a a g a a t t g t a a c a a a a a a c c c c g c c a c a c g t g a a a c a c c g t c t c c t c g t c c t g a c t c c t a t t c t t a a c a t t g t t t t t t g g g g c g g

5904 c c g g c g g g g t t t t t t g t t a a t t a a g g c c g c c c c a a a a a a c a a t t a a t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.