Addgene AiP2028 - pAAV-AiE2518m-minBG-SYFP2-WPRE-BGHpA Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: AiP2028 - pAAV-AiE2518m-minBG-SYFP2-WPRE-BGHpA Sequencing Result


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PciI (4619) pBR322ori-F (4464 .. 4483) AlwNI (4210) BmrI (3691) BsaI (3665) AseI (3556) FspI (3508) PvuI (3362) ScaI (3250) Amp-R (3162 .. 3181) XmnI (3131) AatII (2812) ZraI (2810) pBRforEco (2753 .. 2771) EcoO109I (2751) pGEX 3' (2693 .. 2715) pRS-marker (2574 .. 2593) F1ori-F (2399 .. 2420) DraIII (2335) MluI (139) BstAPI (143) BsmI - SphI (457) NsiI (459) MfeI (463) Eco53kI (545) SacI (547) BamHI (602) BsaBI * (607) BstBI (615) NheI (620) BmtI (624) AfeI (625) AgeI (629) EGFP-N (687 .. 708) PasI (833) EXFP-R (948 .. 967) BlpI (1263) EGFP-C (1295 .. 1316) BsrGI (1351) SalI (1362) AccI (1363) HincII (1364) AscI (1369) SacII (1377) EcoRI (1384) EcoRV (1392) WPRE-R (1451 .. 1471) PaeR7I - XhoI (1642) PmlI (1867) RsrII (1872) KasI (2028) NarI (2029) SfoI (2030) PluTI (2032) F1ori-R (2189 .. 2208) NgoMIV (2227) NaeI (2229) AiP2028 - pAAV-AiE2518m-minBG-SYFP2-WPRE-BGHpA 4624 bp

Sequence View

60 AAV2 ITR (alternate) c c t g c a g g c a g c t g c g c g c t c g c t c g c t c a c t g a g g c c g c c c g g g c g t c g g g c g a c c t t t g g a c g t c c g t c g a c g c g c g a g c g a g c g a g t g a c t c c g g c g g g c c c g c a g c c c g c t g g a a a

120 AAV2 ITR (alternate) g g t c g c c c g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a a c t c c a t c a c t c c a g c g g g c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t t g a g g t a g t g a

BstAPIMluI 180 AAV2 ITR (alternate) a g g g g t t c c t g c g g c c g c a c g c g t t g c t a a a t t a a g c t c t a c c c a c a g a t a g t t t a t g g t t c c c c a a g g a c g c c g g c g t g c g c a a c g a t t t a a t t c g a g a t g g g t g t c t a t c a a a t a c c a

240 a t a t a c t t t g c a c t g a a a a c t c c a t t c a t t t c t a a g g c a a a t g a a t a a a t c c a g t g a t a a t a t a t g a a a c g t g a c t t t t g a g g t a a g t a a a g a t t c c g t t t a c t t a t t t a g g t c a c t a t t

300 t a a a c t c t t a a a g a g a t g a a a c a a g a a g a g a a a a t t t t g g c t a g g c a c a g a g a g a a t a t t a t t t g a g a a t t t c t c t a c t t t g t t c t t c t c t t t t a a a a c c g a t c c g t g t c t c t c t t a t a a

360 t a t a a a g g g a t a t g c a a c c t a c t g a g g t a c a t t t t a g g a a c c a t g g t a t t a t t a a g c t c a a t a t t t c c c t a t a c g t t g g a t g a c t c c a t g t a a a a t c c t t g g t a c c a t a a t a a t t c g a g t

420 a c c t a g t t c a g a g c c a t g a g a a t a a a t t a a a a a c a t t a t t c a c t g a a g c t g a a t t t a a t c t g g a t c a a g t c t c g g t a c t c t t a t t t a a t t t t t g t a a t a a g t g a c t t c g a c t t a a a t t a g

MfeIBsmISphINsiI 480 a a t g t a t a t a t g a c c a a a t t t g a a g a c t a t t a g c a t g c a t t c c a a t t g a g a a t t t t t c a c t t a c a t a t a t a c t g g t t t a a a c t t c t g a t a a t c g t a c g t a a g g t t a a c t c t t a a a a a g t g

540 t a a a t a a a t t c t g a a t g t t a g c a c t t a g t a t a g c a a a g a t a t a c t g c t a a a a a t t g t c t t a t t t a t t t a a g a c t t a c a a t c g t g a a t c a t a t c g t t t c t a t a t g a c g a t t t t t a a c a g a a

Eco53kISacI 600 c g g a g c t c g g g c t g g g c a t a a a a g t c a g g g c a g a g c c a t c t a t t g c t t a c a t t t g c t t c t g c c t c g a g c c c g a c c c g t a t t t t c a g t c c c g t c t c g g t a g a t a a c g a a t g t a a a c g a a g a

AgeIBstBINheIBmtIAfeIBamHIBsaBI* 660 1 M 1a V 5 SKGE SYFP2 Kozak sequence MVSKGE *RDGGHHALAL g g g a t c c a g a t c t t t c g a a g c t a g c g c t a c c g g t c g c c a c c a t g g t g a g c a a g g g c g a g g c c c t a g g t c t a g a a a g c t t c g a t c g c g a t g g c c a g c g g t g g t a c c a c t c g t t c c c g c t c c

720 10 15 20 25 ELFTGVVPILVELDGDVNGH SYFP2 G A C C A G C T C G A C C T G C C G C T G C EGFP-N ELFTGVVPILVELDGDVNGH LQEGPHHGDQDLQVAVYVAV a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t

780 30 35 40 45 KFSVSGEGEGDATYGKLTLK SYFP2 KFSVSGEGEGDATYGKLTLK LEAHGALALAIGGVALQGQL a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g c t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c g

PasI 840 50 55 60 65 LICTTGKLPVPWPTLVTTLG SYFP2 LICTTGKLPVPWPTLVTTLG QDAGGALQGHGPGGEHGGQA t g a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g g g c t a c t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c c c g a

900 70 75 80 85 YGVQCFARYPDHMKQHDFFK SYFP2 YGVQCFARYPDHMKQHDFFK VAHLAEGAVGVVHLLVVEEL a c g g c g t g c a g t g c t t c g c c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t t g c c g c a c g t c a c g a a g c g g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a

960 90 95 100 105 SAMPEGYVQERTIFFKDDGN SYFP2 C T G C T G C C G T T G A EXFP-R SAMPEGYVQERTIFFKDDGN GGHGFAVDLLAGDEELVVAV c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a

1020 110 115 120 125 YKTRAEVKFEGDTLVNRIEL SYFP2 T G T T C T G EXFP-R YKTRAEVKFEGDTLVNRIEL VLGAGLHLELAVGQHVADLQ a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t

1080 130 135 140 145 KGIDFKEDGNILGHKLEYNY SYFP2 KGIDFKEDGNILGHKLEYNY LADVELLVAVDQPVLQLVVV a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t

1140 150 155 160 165 NSHNVYITADKQKNGIKANF SYFP2 NSHNVYITADKQKNGIKANF VAVVDIDGGVLLLVADLGVE a c a g c c a c a a c g t c t a t a t c a c c g c c g a c a a g c a g a a g a a c g g c a t c a a g g c c a a c t t c a t g t c g g t g t t g c a g a t a t a g t g g c g g c t g t t c g t c t t c t t g c c g t a g t t c c g g t t g a a g t

1200 170 175 180 185 KIRHNIEDGGVQLADHYQQN SYFP2 KIRHNIEDGGVQLADHYQQN LDAVVDLVAAHLEGVVVLLV a g a t c c g c c a c a a c a t c g a g g a c g g c g g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a t c t a g g c g g t g t t g t a g c t c c t g c c g c c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t

1260 190 195 200 205 TPIGDGPVLLPDNHYLSYQS SYFP2 TPIGDGPVLLPDNHYLSYQS GGDAVAGHQQGVVVVQAVLG c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c t a c c a g t c c a g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g a t g g t c a g g t

BlpI 1320 210 215 220 225 KLSKDPNEKRDHMVLLEFVT SYFP2 C A T G G T C C T G C T G G A G T T C G T G EGFP-C KLSKDPNEKRDHMVLLEFVT LQAFVGVLLAIVHDQQLEHG a g c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g t c g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c

SacIIAscISalIAccIHincIIBsrGI 1380 230 235 AAGITLGMDELYK * SYFP2 AAGITLGMDELYK* GGPDSEAHVLQVLLDVARRP c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a g t c g a c g g c g c g c c g c g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t c a g c t g c c g c g c g g c g c c g

EcoRVEcoRI 1440 RSNSIM c g c g a a t t c g a t a t c a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a g c g c t t a a g c t a t a g t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t

1500 A C A A C G A G G A A A A T G C G A T A C WPRE-R t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g

1560 a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t a g t t c t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a t c a a g

1620 t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a

PaeR7IXhoI 1680 bGH poly(A) signal t g g g c a c t g a c a a t t c c g t g g c t c g a g a g a t c t t c g a c t g t g c c t t c t a g t t g c c a g c c a a c c c g t g a c t g t t a a g g c a c c g a g c t c t c t a g a a g c t g a c a c g g a a g a t c a a c g g t c g g t

1740 bGH poly(A) signal t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g

1800 bGH poly(A) signal c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c

1860 g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g a g c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c t c

RsrIIPmlI 1920 AAV2 ITR AAV2 ITR a t c t c a c g t g c g g a c c g a g c g g c c g c a g g a a c c c c t a g t g a t g g a g t t g g c c a c t c c c t c t a g a g t g c a c g c c t g g c t c g c c g g c g t c c t t g g g g a t c a c t a c c t c a a c c g g t g a g g g a g

1980 AAV2 ITR AAV2 ITR t c t g c g c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c c g a c g c c c g g g c t t a g a c g c g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g g c t g c g g g c c c g a a

KasINarISfoIPluTI 2040 AAV2 ITR AAV2 ITR t g c c c g g g c g g c c t c a g t g a g c g a g c g a g c g c g c a g c t g c c t g c a g g g g c g c c t g a t g c g a c g g g c c c g c c g g a g t c a c t c g c t c g c t c g c g c g t c g a c g g a c g t c c c c g c g g a c t a c g c

2100 g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a c g t c a a a g c a a c c a t a g c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t g c a g t t t c g t t g g t a t c

2160 f1 ori t a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c a t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g

2220 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R g c t a c a c t t g c c a g c g c c t t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c c g a t g t g a a c g g t c g c g g a a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g

NgoMIVNaeI 2280 f1 ori a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a

DraIII 2340 f1 ori a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t t g g g t g a t g g t t c a c g t a g t g g g t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a a c c c a c t a c c a a g t g c a t c a c c c

2400 f1 ori G T F1ori-F c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a

2460 f1 ori G G A C T C T T G T T C C A A A C T G G F1ori-F g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c t c t a t c t c g g g c t a t t c t t t t g a t t t a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g a g a t a g a g c c c g a t a a g a a a a c t a a a t

2520 f1 ori t a a g g g a t t t t g c c g a t t t c g g t c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a t t c c c t a a a a c g g c t a a a g c c a g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a

2580 f1 ori A T G T T A G pRS-marker a a c g c g a a t t t t a a c a a a a t a t t a a c g t t t a c a a t t t t a t g g t g c a c t c t c a g t a c a a t c t t g c g c t t a a a a t t g t t t t a t a a t t g c a a a t g t t a a a a t a c c a c g t g a g a g t c a t g t t a g

2640 A C G A G A C T A C G G C pRS-marker t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g

2700 C C G G G A G C pGEX 3' t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g

EcoO109I 2760 T G C A T G T G T C A G A G G pGEX 3' C G G A G C A C pBRforEco t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c

ZraIAatII 2820 T A T G C G G A T A A pBRforEco a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g

2880 AmpR promoter a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a

2940 AmpR promoter a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c

3000 1 5 10 15 signal sequence MSIQHFRVALIPFFAAFCL AmpR promoter AmpR MSIQHFRVALIPFFAAFCL a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a

3060 20 signal sequence PVFA 25 30 35 HPETLVKVKDAEDQLG AmpR PVFAHPETLVKVKDAEDQLG c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a

3120 40 45 50 55 ARVGYIELDLNSGKILESFR AmpR ARVGYIELDLNSGKILESFR g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g

XmnI 3180 60 65 70 75 PEERFPMMSTFKVLLCGAVL AmpR C G A T A C A C C G C G C C A T A A T Amp-R PEERFPMMSTFKVLLCGAVL c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t

3240 80 85 90 95 SRIDAGQEQLGRRIHYSQND AmpR A Amp-R SRIDAGQEQLGRRIHYSQND t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g

ScaI 3300 100 105 110 115 LVEYSPVTEKHLTDGMTVRE AmpR LVEYSPVTEKHLTDGMTVRE t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t

3360 120 125 130 135 LCSAAITMSDNTAANLLLTT AmpR LCSAAITMSDNTAANLLLTT t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c

PvuI 3420 140 145 150 155 IGGPKELTAFLHNMGDHVTR AmpR IGGPKELTAFLHNMGDHVTR a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g

3480 160 165 170 175 LDRWEPELNEAIPNDERDTT AmpR LDRWEPELNEAIPNDERDTT c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c

FspI 3540 180 185 190 195 MPVAMATTLRKLLTGELLTL AmpR MPVAMATTLRKLLTGELLTL a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t

AseI 3600 200 205 210 215 ASRQQLIDWMEADKVAGPLL AmpR ASRQQLIDWMEADKVAGPLL g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c

3660 220 225 230 235 RSALPAGWFIADKSGAGERG AmpR RSALPAGWFIADKSGAGERG c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c

BmrIBsaI 3720 240 245 250 255 SRGIIAALGPDGKPSRIVVI AmpR SRGIIAALGPDGKPSRIVVI t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g

3780 260 265 270 275 YTTGSQATMDERNRQIAEIG AmpR YTTGSQATMDERNRQIAEIG t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a

3840 280 285 ASLIKHW* AmpR ASLIKHW* g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a

3900 g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g

3960 a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c

4020 ori a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t

4080 ori a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c

4140 ori a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c

4200 ori t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c

AlwNI 4260 ori t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t

4320 ori t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g

4380 ori t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g

4440 ori a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t

4500 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a

4560 ori c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c

PciI 4620 ori a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g

4624 a t g t t a c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.