Addgene H75S SUMO1 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: H75S SUMO1 Sequencing Result


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FspAI (5640) MscI * (5630) AvaI - BsoBI (5609) StyI (5553) BsmI (5543) BfuAI - BspMI (5238) NruI (5158) EagI (5123) PshAI (4900) HpaI (4561) EcoRV (4505) BssHII (4466) ApaI (4266) PspOMI (4262) BstEII (4236) MluI (4055) LacI-R (3724 .. 3743) EcoNI (3590) pBRrevBam (3402 .. 3421) SgrAI (3374) BglII (3333) T7 (3299 .. 3318) T7 promoter XbaI (3267) RBS 8xHis TEV site NsiI (3178) EcoRI (3168) BstBI (2947) BmgBI (2888) BseRI (2883) Bpu10I (76) BspEI * (159) pGEX 3' (595 .. 617) PflFI - Tth111I (715) BsaAI (722) pRS-marker (758 .. 777) BspQI - SapI (851) L4440 (857 .. 874) PciI (967) pBR322ori-F (1108 .. 1127) AlwNI (1383) AhdI (1860) BsaI (1921) PstI (2105) PvuI (2230) ScaI (2340) Amp-R (2410 .. 2429) SspI (2664) ZraI (2780) AatII (2782) pBRforEco (2820 .. 2838) H75S SUMO1 5689 bp

Sequence View

5ʹ 3ʹ 60 c c t t a c t g g t t a g c a g a a t g a a t c a c c g a t a c g c g a g c g a a c g t g a a g c g a c t g c t g c t g g g a a t g a c c a a t c g t c t t a c t t a g t g g c t a t g c g c t c g c t t g c a c t t c g c t g a c g a c g a c

Bpu10I 120 c a a a a c g t c t g c g a c c t g a g c a a c a a c a t g a a t g g t c t t c g g t t t c c g t g t t t c g t a a a g g t t t t g c a g a c g c t g g a c t c g t t g t t g t a c t t a c c a g a a g c c a a a g g c a c a a a g c a t t t c

BspEI* 180 t c t g g a a a c g c g g a a g t c a g c g c c c t g c a c c a t t a t g t t c c g g a t c t g c a t c g c a g g a t g a g a c c t t t g c g c c t t c a g t c g c g g g a c g t g g t a a t a c a a g g c c t a g a c g t a g c g t c c t a c

240 c t g c t g g c t a c c c t g t g g a a c a c c t a c a t c t g t a t t a a c g a a g c g c t g g c a t t g a c c c t g g a c g a c c g a t g g g a c a c c t t g t g g a t g t a g a c a t a a t t g c t t c g c g a c c g t a a c t g g g a c

300 a g t g a t t t t t c t c t g g t c c c g c c g c a t c c a t a c c g c c a g t t g t t t a c c c t c a c a a c g t t c t c a c t a a a a a g a g a c c a g g g c g g c g t a g g t a t g g c g g t c a a c a a a t g g g a g t g t t g c a a g

360 c a g t a a c c g g g c a t g t t c a t c a t c a g t a a c c c g t a t c g t g a g c a t c c t c t c t c g t t t c a t g t c a t t g g c c c g t a c a a g t a g t a g t c a t t g g g c a t a g c a c t c g t a g g a g a g a g c a a a g t a

420 1 MTKQ rop c g g t a t c a t t a c c c c c a t g a a c a g a a a t c c c c c t t a c a c g g a g g c a t c a g t g a c c a a a c a g c c a t a g t a a t g g g g g t a c t t g t c t t t a g g g g g a a t g t g c c t c c g t a g t c a c t g g t t t g t

480 5 10 15 20 EKTALNMARFIRSQTLTLLE rop g g a a a a a a c c g c c c t t a a c a t g g c c c g c t t t a t c a g a a g c c a g a c a t t a a c g c t t c t g g a c c t t t t t t g g c g g g a a t t g t a c c g g g c g a a a t a g t c t t c g g t c t g t a a t t g c g a a g a c c t

540 25 30 35 40 KLNELDADEQADICESLHDH rop g a a a c t c a a c g a g c t g g a c g c g g a t g a a c a g g c a g a c a t c t g t g a a t c g c t t c a c g a c c a c t t t g a g t t g c t c g a c c t g c g c c t a c t t g t c c g t c t g t a g a c a c t t a g c g a a g t g c t g g t

600 45 50 55 60 ADELYRSCLARFGDDGENL* rop G G A G A C pGEX 3' c g c t g a t g a g c t t t a c c g c a g c t g c c t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g g c g a c t a c t c g a a a t g g c g t c g a c g g a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c

660 rop T G T G T A C G T C G A G G G C C pGEX 3' a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t

Tth111IPflFI 720 bom a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g t c g g g g c g c a g c c a t g a c c c a g t c t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c a g c c c c g c g t c g g t a c t g g g t c a g

BsaAI 780 bom C G G C A T C A G A G C A G A T T G T A pRS-marker a c g t a g c g a t a g c g g a g t g t a t a c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g t g c a t c g c t a t c g c c t c a c a t a t g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c

840 bom a g a g t g c a c c a t t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a t c t c a c g t g g t a a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t

BspQISapI 900 bom G A G C G A G T G A C T G A G C G A L4440 g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c

960 c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c

PciI 1020 g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g

1080 ori t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g

1140 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g

1200 ori t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a

1260 ori c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c

1320 ori t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a

1380 ori c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c

AlwNI 1440 ori c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a

1500 ori g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c c a c c g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g

1560 ori c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t

1620 ori g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c

1680 a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t

1740 t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t

1800 285 *WHKI AmpR *WHKI g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t

AhdI 1860 280 275 270 265 LSAGIEAIQRNREDMTAQSG AmpR LSAGIEAIQRNREDMTAQSG t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g

1920 260 255 250 245 TTYIVVIRSPKGDPGLAAII AmpR TTYIVVIRSPKGDPGLAAII c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t

BsaI 1980 240 235 230 225 GRSGREGAGSKDAIFWGAPL AmpR GRSGREGAGSKDAIFWGAPL t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t

2040 220 215 210 205 ASRLLPGAVKDAEMWDILQQ AmpR ASRLLPGAVKDAEMWDILQQ g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a

2100 200 195 190 185 RSALTLLEGTLLKRLTTAMA AmpR RSALTLLEGTLLKRLTTAMA g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c

PstI 2160 180 175 170 165 APMTTDREDNPIAENLEPEW AmpR APMTTDREDNPIAENLEPEW c t g c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c g a c g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g

2220 160 155 150 145 RDLRTVHDGMNHLFATLEKP AmpR RDLRTVHDGMNHLFATLEKP a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c

PvuI 2280 140 135 130 125 GGITTLLLNAATNDSMTIAA AmpR GGITTLLLNAATNDSMTIAA g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c

ScaI 2340 120 115 110 105 SCLERVTMGDTLHKETVPSY AmpR SCLERVTMGDTLHKETVPSY c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a

2400 100 95 90 85 EVLDNQSYHIRRGLQEQGAD AmpR EVLDNQSYHIRRGLQEQGAD a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a

2460 80 75 70 65 VRSLVAGCLLVKFTSMMPFR AmpR A T A A T A C C G C G C C A C A T A G C Amp-R VRSLVAGCLLVKFTSMMPFR c a a c a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t g t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g

2520 60 55 50 45 EEPRFSELIKGSNLDLEIYG AmpR EEPRFSELIKGSNLDLEIYG g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g

2580 40 35 30 25 VRAGLQDEADKVKVLTEPH A AmpR VRAGLQDEADKVKVLTEPHA c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c

2640 20 15 10 5 signal sequence FVPLCFAAFFPILAVRFHQI AmpR FVPLCFAAFFPILAVRFHQI c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t

SspI 2700 1 signal sequ... SM AmpR AmpR promoter SM t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t

2760 AmpR promoter g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g

ZraIAatII 2820 A pBRforEco c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t

2880 (in frame with TEV site) *GGTQEQYV A T A G G C G T A T C A C G A G G C pBRforEco a t a g g c g t a t c a c g a g g c c c t t t c g g g c t t t g t t a t c c g c c g g t c t g t t c c t g g t a t a c t t a t c c g c a t a g t g c t c c g g g a a a g c c c g a a a c a a t a g g c g g c c a g a c a a g g a c c a t a t g a

BmgBIBseRI 2940 (in frame with TEV site) EIVDEEEMGLEKPTSNDAIR t c a a t c a c g t c c t c c t c t t c c a t a c c c a g c t c t t t a g g a g t a g a g t t g t c c g c g a t g c g c a g t t a g t g c a g g a g g a g a a g g t a t g g g t c g a g a a a t c c t c a t c t c a a c a g g c g c t a c g c g

BstBI 3000 (in frame with TEV site) QGEFLFRLSNMPVGQRQCYS t g g c c t t c g a a c a g g a a g c g t a a g c t g t t c a t c g g a a c a c c c t g g c g c t g g c a a t a c g a t a c c g g a a g c t t g t c c t t c g c a t t c g a c a a g t a g c c t t g t g g g a c c g c g a c c g t t a t g c t a

3060 (in frame with TEV site) EKLKKLHTTMKVKFHIESSD t c c t t c a g c t t t t t c a g a t g c g t a g t c a t c t t a a c t t t g a a a t g a a t c t c g c t a c t g t c c a g g a a g t c g a a a a a g t c t a c g c a t c a g t a g a a t t g a a a c t t t a c t t a g a g c g a t g a c a g g

3120 (in frame with TEV site) QGIVKLKIYEGEKKDGLDET t g t c c a a t c a c t t t c a g t t t a a t a t a c t c c c c t t c t t t t t t a t c a c c a a g a t c c t c c g t t a c a g g t t a g t g a a a g t c a a a t t a t a t g a g g g g a a g a a a a a a t a g t g g t t c t a g g a g g c a a

NsiIEcoRI 3180 (in frame with TEV site) SPKAEQDSMSGGWSGGFEHM TEV site g a g g g t t t g g c t t c c t g a t c a c t c a t a g a a c c a c c c c a t g a g c c g c c g a a t t c a t g c a t g c t c c c a a a c c g a a g g a c t a g t g a g t a t c t t g g t g g g g t a c t c g g c g g c t t a a g t a c g t a c

3240 5 1 GQFYLNE 5 1 HHHHHHHH TEV site 8xHis RBS c c c t g a a a a t a c a g g t t t t c g t g a t g a t g g t g g t g a t g g t g g t g c a t g a t a t g t a t a t c t g g g a c t t t t a t g t c c a a a a g c a c t a c t a c c a c c a c t a c c a c c a c g t a c t a t a c a t a t a g a

XbaI 3300 RBS lac operator T7 promoter G G T7 c c t t c t t a a a g t t a a a c a a a a t t a t t t c t a g a g g g g a a t t g t t a t c c g c t c a c a a t t c c c g g a a g a a t t t c a a t t t g t t t t a a t a a a g a t c t c c c c t t a a c a a t a g g c g a g t g t t a a g g g

BglII 3360 T7 promoter G A T A T C A C T C A G C A T A A T T7 c t a t a g t g a g t c g t a t t a a t t t c g c g g g a t c g a g a t c t c g a t c c t c t a c g c c g g a c g c a t g a t a t c a c t c a g c a t a a t t a a a g c g c c c t a g c t c t a g a g c t a g g a g a t g c g g c c t g c g t a

SgrAI 3420 G G A T A T A G C G G C T G T A G T G pBRrevBam c g t g g c c g g c a t c a c c g g c g c c a c a g g t g c g g t t g c t g g c g c c t a t a t c g c c g a c a t c a c g c a c c g g c c g t a g t g g c c g c g g t g t c c a c g c c a a c g a c c g c g g a t a t a g c g g c t g t a g t g

3480 G pBRrevBam MSACFGVGM c g a t g g g g a a g a t c g g g c t c g c c a c t t c g g g c t c a t g a g c g c t t g t t t c g g c g t g g g t a t g c t a c c c c t t c t a g c c c g a g c g g t g a a g c c c g a g t a c t c g c g a a c a a a g c c g c a c c c a t a

3540 VAGPVAGGLLGAISLHAPFL g g t g g c a g g c c c c g t g g c c g g g g g a c t g t t g g g c g c c a t c t c c t t g c a t g c a c c a t t c c t c c a c c g t c c g g g g c a c c g g c c c c c t g a c a a c c c g c g g t a g a g g a a c g t a c g t g g t a a g g a

EcoNI 3600 AAAVLNGLNLLLGCFLMQES t g c g g c g g c g g t g c t c a a c g g c c t c a a c c t a c t a c t g g g c t g c t t c c t a a t g c a g g a g t c a c g c c g c c g c c a c g a g t t g c c g g a g t t g g a t g a t g a c c c g a c g a a g g a t t a c g t c c t c a g

3660 lacI promoter HKGERRDPGHHRMAQNLSRY g c a t a a g g g a g a g c g t c g a g a t c c c g g a c a c c a t c g a a t g g c g c a a a a c c t t t c g c g g t a c g t a t t c c c t c t c g c a g c t c t a g g g c c t g t g g t a g c t t a c c g c g t t t t g g a a a g c g c c a t

3720 1 5 MKPVT lacI promoter lacI GMIAPGRESIQGGECETSNV t g g c a t g a t a g c g c c c g g a a g a g a g t c a a t t c a g g g t g g t g a a t g t g a a a c c a g t a a c g t a c c g t a c t a t c g c g g g c c t t c t c t c a g t t a a g t c c c a c c a c t t a c a c t t t g g t c a t t g c a

3780 10 15 20 25 LYDVAEYAGVSYQTVSRVVN lacI T G C T A C A G C G T C T C A T A C G G LacI-R IRCRRVCRCLLSDRFPRGEP t a t a c g a t g t c g c a g a g t a t g c c g g t g t c t c t t a t c a g a c c g t t t c c c g c g t g g t g a a c c a t a t g c t a c a g c g t c t c a t a c g g c c a c a g a g a a t a g t c t g g c a a a g g g c g c a c c a c t t g g

3840 30 35 40 45 QASHVSAKTREKVEAAMAEL lacI GQPRFCENAGKSGSGDGGAE MAEL a g g c c a g c c a c g t t t c t g c g a a a a c g c g g g a a a a a g t g g a a g c g g c g a t g g c g g a g c t g a t c c g g t c g g t g c a a a g a c g c t t t t g c g c c c t t t t t c a c c t t c g c c g c t a c c g c c t c g a c t

3900 50 55 60 65 NYIPNRVAQQLAGKQSLLIG lacI LHSQPRGTTTGGQTVVADWR NYIPNRVAQQLAGKQSLLIG a t t a c a t t c c c a a c c g c g t g g c a c a a c a a c t g g c g g g c a a a c a g t c g t t g c t g a t t g g c g t a a t g t a a g g g t t g g c g c a c c g t g t t g t t g a c c g c c c g t t t g t c a g c a a c g a c t a a c c g c

3960 70 75 80 85 VATSSLALHAPSQIVAAIKS lacI CHLQSGPARAVANCRGD* VATSSLALHAPSQIVAAIKS t t g c c a c c t c c a g t c t g g c c c t g c a c g c g c c g t c g c a a a t t g t c g c g g c g a t t a a a t c t c a a c g g t g g a g g t c a g a c c g g g a c g t g c g c g g c a g c g t t t a a c a g c g c c g c t a a t t t a g a g

4020 90 95 100 105 RADQLGASVVVSMVERSGVE lacI RADQLGASVVVSMVERSGVE g c g c c g a t c a a c t g g g t g c c a g c g t g g t g g t g t c g a t g g t a g a a c g a a g c g g c g t c g a a g c g c g g c t a g t t g a c c c a c g g t c g c a c c a c c a c a g c t a c c a t c t t g c t t c g c c g c a g c t t c

MluI 4080 110 115 120 125 ACKAAVHNLLAQRVSGLIIN lacI ACKAAVHNLLAQRVSGLIIN c c t g t a a a g c g g c g g t g c a c a a t c t t c t c g c g c a a c g c g t c a g t g g g c t g a t c a t t a a c t g g a c a t t t c g c c g c c a c g t g t t a g a a g a g c g c g t t g c g c a g t c a c c c g a c t a g t a a t t g a

4140 130 135 140 145 YPLDDQDAIAVEAACTNVPA lacI YPLDDQDAIAVEAACTNVPA a t c c g c t g g a t g a c c a g g a t g c c a t t g c t g t g g a a g c t g c c t g c a c t a a t g t t c c g g c g t t a g g c g a c c t a c t g g t c c t a c g g t a a c g a c a c c t t c g a c g g a c g t g a t t a c a a g g c c g c a

4200 150 155 160 165 LFLDVSDQTPINSIIFSHED lacI LFLDVSDQTPINSIIFSHED t a t t t c t t g a t g t c t c t g a c c a g a c a c c c a t c a a c a g t a t t a t t t t c t c c c a t g a a g a c g a t a a a g a a c t a c a g a g a c t g g t c t g t g g g t a g t t g t c a t a a t a a a a g a g g g t a c t t c t g c

BstEII 4260 170 175 180 185 GTRLGVEHLVALGHQQIALL lacI GTRLGVEHLVALGHQQIALL g t a c g c g a c t g g g c g t g g a g c a t c t g g t c g c a t t g g g t c a c c a g c a a a t c g c g c t g t t a g c a t g c g c t g a c c c g c a c c t c g t a g a c c a g c g t a a c c c a g t g g t c g t t t a g c g c g a c a a t c

PspOMIApaI 4320 190 195 200 205 AGPLSSVSARLRLAGWHKYL lacI AGPLSSVSARLRLAGWHKYL c g g g c c c a t t a a g t t c t g t c t c g g c g c g t c t g c g t c t g g c t g g c t g g c a t a a a t a t c t c a g c c c g g g t a a t t c a a g a c a g a g c c g c g c a g a c g c a g a c c g a c c g a c c g t a t t t a t a g a g t

4380 210 215 220 225 TRNQIQPIAEREGDWSAMSG lacI TRNQIQPIAEREGDWSAMSG c t c g c a a t c a a a t t c a g c c g a t a g c g g a a c g g g a a g g c g a c t g g a g t g c c a t g t c c g g t t g a g c g t t a g t t t a a g t c g g c t a t c g c c t t g c c c t t c c g c t g a c c t c a c g g t a c a g g c c a a

4440 230 235 240 245 FQQTMQMLNEGIVPTAMLVA lacI FQQTMQMLNEGIVPTAMLVA t t c a a c a a a c c a t g c a a a t g c t g a a t g a g g g c a t c g t t c c c a c t g c g a t g c t g g t t g c c a a a g t t g t t t g g t a c g t t t a c g a c t t a c t c c c g t a g c a a g g g t g a c g c t a c g a c c a a c g g t

BssHII 4500 250 255 260 265 NDQMALGAMRAITESGLRVG lacI NDQMALGAMRAITESGLRVG a c g a t c a g a t g g c g c t g g g c g c a a t g c g c g c c a t t a c c g a g t c c g g g c t g c g c g t t g g t g t g c t a g t c t a c c g c g a c c c g c g t t a c g c g c g g t a a t g g c t c a g g c c c g a c g c g c a a c c a c

EcoRV 4560 270 275 280 285 ADISVVGYDDTEDSSCYIPP lacI ADISVVGYDDTEDSSCYIPP c g g a t a t c t c g g t a g t g g g a t a c g a c g a t a c c g a a g a c a g c t c a t g t t a t a t c c c g c c g t g c c t a t a g a g c c a t c a c c c t a t g c t g c t a t g g c t t c t g t c g a g t a c a a t a t a g g g c g g c a

HpaI 4620 290 295 300 305 LTTIKQDFRLLGQTSVDRLL lacI LTTIKQDFRLLGQTSVDRLL *VPNEGAPAFWRPGSAA t a a c c a c c a t c a a a c a g g a t t t t c g c c t g c t g g g g c a a a c c a g c g t g g a c c g c t t g c t g c a t t g g t g g t a g t t t g t c c t a a a a g c g g a c g a c c c c g t t t g g t c g c a c c t g g c g a a c g a c g

4680 310 315 320 325 QLSQGQAVKGNQLLPVSLVK lacI QLSQGQAVKGNQLLPVSLVK VREPGPPSPCDATARRVPSF a a c t c t c t c a g g g c c a g g c g g t g a a g g g c a a t c a g c t g t t g c c c g t c t c a c t g g t g a a a a t t g a g a g a g t c c c g g t c c g c c a c t t c c c g t t a g t c g a c a a c g g g c a g a g t g a c c a c t t t t

4740 330 335 340 345 RKTTLAPNTQTASPRALADS lacI RKTTLAPNTQTASPRALADS FFWGPAWYAFRREGRTPRNM g a a a a a c c a c c c t g g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t c t t t t t g g t g g g a c c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a

4800 350 355 360 LMQLARQVSRLESGQ* lacI LMQLARQVSRLESGQ* LAAPVVPKGVPFRATLAVCN t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a

4860 MPLRAF IYTLESMLCRSRSRHGQSGE a a t g t a a g t t a g c t c a c t c a t t a g g c a c c g g g a t c t c g a c c g a t g c c c t t g a g a g c c t t c t t a c a t t c a a t c g a g t g a g t a a t c c g t g g c c c t a g a g c t g g c t a c g g g a a c t c t c g g a a g

PshAI 4920 NPVSSFRWARGMTIVAALMT VWDAGEPPRPAHSDDGCKHS a a c c c a g t c a g c t c c t t c c g g t g g g c g c g g g g c a t g a c t a t c g t c g c c g c a c t t a t g a c t t t g g g t c a g t c g a g g a a g g c c a c c c g c g c c c c g t a c t g a t a g c a g c g g c g t g a a t a c t g a

4980 VFFIMQLVGQVPAALWVIFG DEKDHLEYSLHRCREPDNEA g t c t t c t t t a t c a t g c a a c t c g t a g g a c a g g t g c c g g c a g c g c t c t g g g t c a t t t t c g g c c a g a a g a a a t a g t a c g t t g a g c a t c c t g t c c a c g g c c g t c g c g a g a c c c a g t a a a a g c c g

5040 EDRFRWSATMIGLSLAVFGI LVAKAPARRHDAQRKRYESD g a g g a c c g c t t t c g c t g g a g c g c g a c g a t g a t c g g c c t g t c g c t t g c g g t a t t c g g a a t c c t c c t g g c g a a a g c g a c c t c g c g c t g c t a c t a g c c g g a c a g c g a a c g c c a t a a g c c t t a g

5100 LHALAQAFVTGPATKRFGEK QVGESLGEDSTGGGFTEALL t t g c a c g c c c t c g c t c a a g c c t t c g t c a c t g g t c c c g c c a c c a a a c g t t t c g g c g a g a a g a a c g t g c g g g a g c g a g t t c g g a a g c a g t g a c c a g g g c g g t g g t t t g c a a a g c c g c t c t t c

NruIEagI 5160 QAIIAGMAADALGYVLLAFA LGNDGAHRGVRQAVDQQRER c a g g c c a t t a t c g c c g g c a t g g c g g c c g a c g c g c t g g g c t a c g t c t t g c t g g c g t t c g c g g t c c g g t a a t a g c g g c c g t a c c g c c g g c t g c g c g a c c c g a t g c a g a a c g a c c g c a a g c g c

5220 TRGWMAFPIMILLASGGIGM RSAPHGEGNHNKESGAADPH a c g c g a g g c t g g a t g g c c t t c c c c a t t a t g a t t c t t c t c g c t t c c g g c g g c a t c g g g a t g t g c g c t c c g a c c t a c c g g a a g g g g t a a t a c t a a g a a g a g c g a a g g c c g c c g t a g c c c t a c

BspMIBfuAI 5280 PALQAMLSRQVDDDHQGQLQ GRQLGHQGPLYIVVM c c c g c g t t g c a g g c c a t g c t g t c c a g g c a g g t a g a t g a c g a c c a t c a g g g a c a g c t t c a a g g g c g c a a c g t c c g g t a c g a c a g g t c c g t c c a t c t a c t g c t g g t a g t c c c t g t c g a a g t t

5340 GSLAALTSLTSIIGPLIVTA g g a t c g c t c g c g g c t c t t a c c a g c c t a a c t t c g a t c a t t g g a c c g c t g a t c g t c a c g g c g c c t a g c g a g c g c c g a g a a t g g t c g g a t t g a a g c t a g t a a c c t g g c g a c t a g c a g t g c c g c

5400 IYAASASTWNGLAWIVGAAL a t t t a t g c c g c c t c g g c g a g c a c a t g g a a c g g g t t g g c a t g g a t t g t a g g c g c c g c c c t a t a a a t a c g g c g g a g c c g c t c g t g t a c c t t g c c c a a c c g t a c c t a a c a t c c g c g g c g g g a t

5460 YLVCLPALRRGAWSRATST* t a c c t t g t c t g c c t c c c c g c g t t g c g t c g c g g t g c a t g g a g c c g g g c c a c c t c g a c c t g a a t g g a a c a g a c g g a g g g g c g c a a c g c a g c g c c a c g t a c c t c g g c c c g g t g g a g c t g g a c t

5520 a t g g a a g c c g g c g g c a c c t c g c t a a c g g a t t c a c c a c t c c a a g a a t t g g a g c c a a t c a a t t a c c t t c g g c c g c c g t g g a g c g a t t g c c t a a g t g g t g a g g t t c t t a a c c t c g g t t a g t t a

StyIBsmI 5580 t c t t g c g g a g a a c t g t g a a t g c g c a a a c c a a c c c t t g g c a g a a c a t a t c c a t c g c g t c c g a g a a c g c c t c t t g a c a c t t a c g c g t t t g g t t g g g a a c c g t c t t g t a t a g g t a g c g c a g g c

FspAIMscI*BsoBIAvaI 5640 c c a t c t c c a g c a g c c g c a c g c g g c g c a t c t c g g g c a g c g t t g g g t c c t g g c c a c g g g t g c g g t a g a g g t c g t c g g c g t g c g c c g c g t a g a g c c c g t c g c a a c c c a g g a c c g g t g c c c a c g

3ʹ 5ʹ 5689 g c a t g a t c g t g c t c c t g t c g t t g a g g a c c c g g c t a g g c t g g c g g g g t t g c g t a c t a g c a c g a g g a c a g c a a c t c c t g g g c c g a t c c g a c c g c c c c a a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.