Addgene pTrc-clbS_Dickeya Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pTrc-clbS_Dickeya Sequencing Result


Map View

LacI-R (4582 .. 4601) NsiI (4476) SphI (4474) MauBI (4452) pGEX 3' (4385 .. 4407) PflFI - Tth111I (4285) BsaAI (4279) BstZ17I (4260) AccI (4259) pRS-marker (4225 .. 4244) NdeI (4208) BspQI - SapI (4146) L4440 (4127 .. 4144) PciI (4029) pBR322ori-F (3874 .. 3893) AlwNI (3620) AhdI (3141) BglI (3023) FspI (2918) PvuI (2772) ScaI (2660) Amp-R (2572 .. 2591) MluI (137) BclI * (151) PspOMI (344) ApaI (348) EcoRV (587) HpaI (643) KasI (776) NarI * (777) SfoI (778) PluTI (780) trc promoter M13/pUC Reverse (1143 .. 1165) lac operator minicistron Eco53kI (1413) SacI (1415) MscI (1439) AgeI (1561) AvaI - BsoBI (1582) BsaBI * (1703) BglII (1704) BmgBI (1766) PaqCI (1832) pBAD Reverse (1868 .. 1885) rrnB T1 terminator rrnB T2 terminator pTrc-clbS_Dickeya 4776 bp

Sequence View

60 75 80 85 90 ALHAPSQIVAAIKSRADQLG lacI LHAPSQIVAAIKSRADQLG c c c t g c a c g c g c c g t c g c a a a t t g t c g c g g c g a t t a a a t c t c g c g c c g a t c a a c t g g g t g g g g a c g t g c g c g g c a g c g t t t a a c a g c g c c g c t a a t t t a g a g c g c g g c t a g t t g a c c c a c

120 95 100 105 110 ASVVVSMVERSGVEACKAAV lacI ASVVVSMVERSGVEACKAAV c c a g c g t g g t g g t g t c g a t g g t a g a a c g a a g c g g c g t c g a a g c c t g t a a a g c g g c g g t g c g g t c g c a c c a c c a c a g c t a c c a t c t t g c t t c g c c g c a g c t t c g g a c a t t t c g c c g c c a c g

BclI*MluI 180 115 120 125 130 HNLLAQRVSGLIINYPLDDQ lacI HNLLAQRVSGLIINYPLDDQ a c a a t c t t c t c g c g c a a c g c g t c a g t g g g c t g a t c a t t a a c t a t c c g c t g g a t g a c c a g g t g t t a g a a g a g c g c g t t g c g c a g t c a c c c g a c t a g t a a t t g a t a g g c g a c c t a c t g g t c c

240 135 140 145 150 DAIAVEAACTNVPALFLDVS lacI DAIAVEAACTNVPALFLDVS a t g c c a t t g c t g t g g a a g c t g c c t g c a c t a a t g t t c c g g c g t t a t t t c t t g a t g t c t c t g t a c g g t a a c g a c a c c t t c g a c g g a c g t g a t t a c a a g g c c g c a a t a a a g a a c t a c a g a g a c

300 155 160 165 170 DQTPINSIIFSHEDGTRLGV lacI DQTPINSIIFSHEDGTRLGV a c c a g a c a c c c a t c a a c a g t a t t a t t t t c t c c c a t g a a g a c g g t a c g c g a c t g g g c g t g g t g g t c t g t g g g t a g t t g t c a t a a t a a a a g a g g g t a c t t c t g c c a t g c g c t g a c c c g c a c c

PspOMIApaI 360 175 180 185 190 EHLVALGHQQIALLAGPLSS lacI EHLVALGHQQIALLAGPLSS a g c a t c t g g t c g c a t t g g g t c a c c a g c a a a t c g c g c t g t t a g c g g g c c c a t t a a g t t c t g t c g t a g a c c a g c g t a a c c c a g t g g t c g t t t a g c g c g a c a a t c g c c c g g g t a a t t c a a g a c

420 195 200 205 210 VSARLRLAGWHKYLTRNQIQ lacI VSARLRLAGWHKYLTRNQIQ t c t c g g c g c g t c t g c g t c t g g c t g g c t g g c a t a a a t a t c t c a c t c g c a a t c a a a t t c a g c a g a g c c g c g c a g a c g c a g a c c g a c c g a c c g t a t t t a t a g a g t g a g c g t t a g t t t a a g t c g

480 215 220 225 230 PIAEREGDWSAMSGFQQTMQ lacI PIAEREGDWSAMSGFQQTMQ c g a t a g c g g a a c g g g a a g g c g a c t g g a g t g c c a t g t c c g g t t t t c a a c a a a c c a t g c a a a g c t a t c g c c t t g c c c t t c c g c t g a c c t c a c g g t a c a g g c c a a a a g t t g t t t g g t a c g t t t

540 235 240 245 250 MLNEGIVPTAMLVANDQMAL lacI MLNEGIVPTAMLVANDQMAL t g c t g a a t g a g g g c a t c g t t c c c a c t g c g a t g c t g g t t g c c a a c g a t c a g a t g g c g c t g g a c g a c t t a c t c c c g t a g c a a g g g t g a c g c t a c g a c c a a c g g t t g c t a g t c t a c c g c g a c c

EcoRV 600 255 260 265 270 GAMRAITESGLRVGADISVV lacI GAMRAITESGLRVGADISVV g c g c a a t g c g c g c c a t t a c c g a g t c c g g g c t g c g c g t t g g t g c g g a t a t c t c g g t a g t g g c g c g t t a c g c g c g g t a a t g g c t c a g g c c c g a c g c g c a a c c a c g c c t a t a g a g c c a t c a c c

HpaI 660 275 280 285 290 GYDDTEDSSCYIPPLTTIKQ lacI GYDDTEDSSCYIPPLTTIKQ g a t a c g a c g a t a c c g a a g a c a g c t c a t g t t a t a t c c c g c c g t t a a c c a c c a t c a a a c a g g c t a t g c t g c t a t g g c t t c t g t c g a g t a c a a t a t a g g g c g g c a a t t g g t g g t a g t t t g t c c

720 295 300 305 310 DFRLLGQTSVDRLLQLSQGQ lacI DFRLLGQTSVDRLLQLSQGQ a t t t t c g c c t g c t g g g g c a a a c c a g c g t g g a c c g c t t g c t g c a a c t c t c t c a g g g c c a g g t a a a a g c g g a c g a c c c c g t t t g g t c g c a c c t g g c g a a c g a c g t t g a g a g a g t c c c g g t c c

KasINarI*SfoIPluTI 780 315 320 325 330 AVKGNQLLPVSLVKRKTTLA lacI AVKGNQLLPVSLVKRKTTLA c g g t g a a g g g c a a t c a g c t g t t g c c c g t c t c a c t g g t g a a a a g a a a a a c c a c c c t g g c g c g c c a c t t c c c g t t a g t c g a c a a c g g g c a g a g t g a c c a c t t t t c t t t t t g g t g g g a c c g c g

840 335 340 345 350 PNTQTASPRALADSLMQLAR lacI PNTQTASPRALADSLMQLAR c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g

900 355 360 QVSRLESGQ* lacI QVSRLESGQ* a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t a a g t t a g c g c g a a t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a t t c a a t c g c g c t t

960 t t g a t c t g g t t t g a c a g c t t a t c a t c g a c t g c a c g g t g c a c c a a t g c t t c t g g c g t c a g g a a c t a g a c c a a a c t g t c g a a t a g t a g c t g a c g t g c c a c g t g g t t a c g a a g a c c g c a g t c c

1020 c a g c c a t c g g a a g c t g t g g t a t g g c t g t g c a g g t c g t a a a t c a c t g c a t a a t t c g t g t c g g t c g g t a g c c t t c g a c a c c a t a c c g a c a c g t c c a g c a t t t a g t g a c g t a t t a a g c a c a g c

1080 c t c a a g g c g c a c t c c c g t t c t g g a t a a t g t t t t t t g c g c c g a c a t c a t a a c g g t t c t g g c g a g t t c c g c g t g a g g g c a a g a c c t a t t a c a a a a a a c g c g g c t g t a g t a t t g c c a a g a c c g

1140 -35 -10 trc promoter lac operator a a a t a t t c t g a a a t g a g c t g t t g a c a a t t a a t c a t c c g g c t c g t a t a a t g t g t g g a a t t g t t t a t a a g a c t t t a c t c g a c a a c t g t t a a t t a g t a g g c c g a g c a t a t t a c a c a c c t t a a c

1200 lac operator rrnG antiterminator A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c g c c g c t g a g a a a a a g c g a a g c g g c a c t g a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g c g g c g a c t c t t t t t c g c t t c g c c g t g a c

1260 rrnG antiterminator c t c t t t a a c a a t t t a t c a g a c a a t c t g t g t g g g c a c t c g a c c g g a a t t a t c g a t t a a c t t g a g a a a t t g t t a a a t a g t c t g t t a g a c a c a c c c g t g a g c t g g c c t t a a t a g c t a a t t g a a

1320 1 5 MYRLNKEE* minicistron t a t t a t t a a a a a t t a a a g a g g t a t a t a t t a a t g t a t c g a t t a a a t a a g g a g g a a t a a a c c a t a a t a a t t t t t a a t t t c t c c a t a t a t a a t t a c a t a g c t a a t t t a t t c c t c c t t a t t t g g

1380 MGVPQTKSELLFAIDKNFTK a t g g g c g t g c c a c a a a c a a a a t c c g a a c t a c t t t t c g c t a t t g a t a a a a a t t t t a c g a a a t a c c c g c a c g g t g t t t g t t t t a g g c t t g a t g a a a a g c g a t a a c t a t t t t t a a a a t g c t t t

MscIEco53kISacI 1440 LIGYLNAIPPELTADKSLDG t t a a t c g g t t a c c t c a a t g c g a t t c c g c c a g a g c t c a c t g c g g a t a a a t c g c t g g a t g g c a a t t a g c c a a t g g a g t t a c g c t a a g g c g g t c t c g a g t g a c g c c t a t t t a g c g a c c t a c c g

1500 HAKGTEMSVCELVSYLLGWN c a t g c a a a a g g c a c g g a g a t g a g t g t t t g t g a g c t t g t t t c g t a t t t g c t g g g a t g g a a c g t a c g t t t t c c g t g c c t c t a c t c a c a a a c a c t c g a a c a a a g c a t a a a c g a c c c t a c c t t g

1560 TLVVKWISGDATGQPVDFPE a c t c t g g t t g t a a a a t g g a t t a g c g g t g a c g c a a c a g g g c a a c c t g t t g a t t t c c c g g a a t g a g a c c a a c a t t t t a c c t a a t c g c c a c t g c g t t g t c c c g t t g g a c a a c t a a a g g g c c t t

BsoBIAvaIAgeI 1620 TGYKWNQLGLLAQKFYFDYK a c c g g t t a c a a g t g g a a t c a g c t c g g g c t t c t g g c g c a g a a a t t t t a c t t t g a t t a c a a a t g g c c a a t g t t c a c c t t a g t c g a g c c c g a a g a c c g c g t c t t t a a a a t g a a a c t a a t g t t t

1680 ELNYQTLINNLQSVKDEIVR g a a t t a a a t t a c c a g a c g t t a a t c a a t a a c c t t c a g t c g g t a a a a g a c g a a a t a g t g a g g c t t a a t t t a a t g g t c t g c a a t t a g t t a t t g g a a g t c a g c c a t t t t c t g c t t t a t c a c t c c

BsaBI*BglII 1740 LIDERTDEDLYGKPWYGKWT c t g a t t g a t g a g c g c a c t g a t g a a g a t c t g t a t g g c a a g c c a t g g t a t g g c a a a t g g a c a g a c t a a c t a c t c g c g t g a c t a c t t c t a g a c a t a c c g t t c g g t a c c a t a c c g t t t a c c t g t

BmgBI 1800 MGRMISLNTSSPYANANGRL a t g g g a c g t a t g a t c t c a t t g a a c a c g t c a t c a c c c t a t g c c a a t g c t a a t g g a a g a t t a t a c c c t g c a t a c t a g a g t a a c t t g t g c a g t a g t g g g a t a c g g t t a c g a t t a c c t t c t a a t

PaqCI 1860 RQWAKNNHLRLK* a g g c a g t g g g c a a a g a a c a a t c a c c t g c g t t t a a a a t g a g t t t t g g c g g a t g a g a g a a g a t c c g t c a c c c g t t t c t t g t t a g t g g a c g c a a a t t t t a c t c a a a a c c g c c t a c t c t c t t c t

1920 G G A C T A T G T C T A A T T T A G pBAD Reverse t t t t c a g c c t g a t a c a g a t t a a a t c a g a a c g c a g a a g c g g t c t g a t a a a a c a g a a t t t g c a a a a g t c g g a c t a t g t c t a a t t t a g t c t t g c g t c t t c g c c a g a c t a t t t t g t c t t a a a c g

1980 c t g g c g g c a g t a g c g c g g t g g t c c c a c c t g a c c c c a t g c c g a a c t c a g a a g t g a a a c g c c g a c c g c c g t c a t c g c g c c a c c a g g g t g g a c t g g g g t a c g g c t t g a g t c t t c a c t t t g c g g

2040 rrnB T1 terminator g t a g c g c c g a t g g t a g t g t g g g g t c t c c c c a t g c g a g a g t a g g g a a c t g c c a g g c a t c a a c a t c g c g g c t a c c a t c a c a c c c c a g a g g g g t a c g c t c t c a t c c c t t g a c g g t c c g t a g t t

2100 rrnB T1 terminator a t a a a a c g a a a g g c t c a g t c g a a a g a c t g g g c c t t t c g t t t t a t c t g t t g t t t g t c g g t g t a t t t t g c t t t c c g a g t c a g c t t t c t g a c c c g g a a a g c a a a a t a g a c a a c a a a c a g c c a c

2160 rrnB T1 terminator a a c g c t c t c c t g a g t a g g a c a a a t c c g c c g g g a g c g g a t t t g a a c g t t g c g a a g c a a c g g t t g c g a g a g g a c t c a t c c t g t t t a g g c g g c c c t c g c c t a a a c t t g c a a c g c t t c g t t g c c

2220 rrnB T2 terminator c c c g g a g g g t g g c g g g c a g g a c g c c c g c c a t a a a c t g c c a g g c a t c a a a t t a a g c a g a a g g g g c c t c c c a c c g c c c g t c c t g c g g g c g g t a t t t g a c g g t c c g t a g t t t a a t t c g t c t t c

2280 rrnB T2 terminator AmpR promoter g c c a t c c t g a c g g a t g g c c t t t t t g c g t t t c t a c a a a c t c t t t t g t t t a t t t t t c t a a a t c g g t a g g a c t g c c t a c c g g a a a a a c g c a a a g a t g t t t g a g a a a a c a a a t a a a a a g a t t t a

2340 AmpR promoter a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c

2400 1 5 10 15 signal sequence MSIQHFRVALIPFFAA AmpR promoter AmpR MSIQHFRVALIPFFAA a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g

2460 20 signal sequence FCLPVFA 25 30 35 HPETLVKVKDAED AmpR FCLPVFAHPETLVKVKDAED a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t

2520 40 45 50 55 QLGARVGYIELDLNSGKILE AmpR QLGARVGYIELDLNSGKILE t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t

2580 60 65 70 75 SFRPEERFPMMSTFKVLLCG AmpR C G A T A C A C C Amp-R SFRPEERFPMMSTFKVLLCG g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c

2640 80 85 90 95 AVLSRVDAGQEQLGRRIHYS AmpR G C G C C A T A A T A Amp-R AVLSRVDAGQEQLGRRIHYS c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g

ScaI 2700 100 105 110 115 QNDLVEYSPVTEKHLTDGMT AmpR QNDLVEYSPVTEKHLTDGMT t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g

2760 120 125 130 135 VRELCSAAITMSDNTAANLL AmpR VRELCSAAITMSDNTAANLL a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a

PvuI 2820 140 145 150 155 LTTIGGPKELTAFLHNMGDH AmpR LTTIGGPKELTAFLHNMGDH t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t

2880 160 165 170 175 VTRLDRWEPELNEAIPNDER AmpR VTRLDRWEPELNEAIPNDER t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c

FspI 2940 180 185 190 195 DTTMPVAMATTLRKLLTGEL AmpR DTTMPVAMATTLRKLLTGEL t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a

3000 200 205 210 215 LTLASRQQLIDWMEADKVAG AmpR LTLASRQQLIDWMEADKVAG a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c

BglI 3060 220 225 230 235 PLLRSALPAGWFIADKSGAG AmpR PLLRSALPAGWFIADKSGAG a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c

3120 240 245 250 255 ERGSRGIIAALGPDGKPSRI AmpR ERGSRGIIAALGPDGKPSRI t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a

AhdI 3180 260 265 270 275 VVIYTTGSQATMDERNRQIA AmpR VVIYTTGSQATMDERNRQIA c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g

3240 280 285 EIGASLIKHW* AmpR EIGASLIKHW* t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a

3300 a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a

3360 t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g

3420 ori c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a

3480 ori g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g

3540 ori t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a

3600 ori g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t t t a g c a c c g c c t a c a t a c c t c g c t c t c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a a a t c g t g g c g g a t g t a t g g a g c g a g a

AlwNI 3660 ori g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t

3720 ori c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g

3780 ori a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c

3840 ori a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a

3900 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g

3960 ori t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c

4020 ori g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g

PciI 4080 t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g

4140 A G C G A G T C A G T G A G L4440 c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c

BspQISapI 4200 bom C G A G L4440 c g a g g a a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c g c t c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g

AccIBstZ17INdeI 4260 bom A T G T T A G A C G A G A C T A C G G C pRS-marker a c a c c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t g t g g c g t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t

Tth111IPflFIBsaAI 4320 bom t a c a c t c c g c t a t c g c t a c g t g a c t g g g t c a t g g c t g c g c c c c g a c a c c c g c c a a c a c c c a t g t g a g g c g a t a g c g a t g c a c t g a c c c a g t a c c g a c g c g g g g c t g t g g g c g g t t g t g g g

4380 g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g

4440 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g g c a g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c c g t c

SphINsiIMauBI 4500 lacIq promoter c a g a t c a a t t c g c g c g c g a a g g c g a a g c g g c a t g c a t t t a c g t t g a c a c c a t c g a a t g g t g t c t a g t t a a g c g c g c g c t t c c g c t t c g c c g t a c g t a a a t g c a a c t g t g g t a g c t t a c c a

4560 lacIq promoter g c a a a a c c t t t c g c g g t a t g g c a t g a t a g c g c c c g g a a g a g a g t c a a t t c a g g g t g g t g a c g t t t t g g a a a g c g c c a t a c c g t a c t a t c g c g g g c c t t c t c t c a g t t a a g t c c c a c c a c t

4620 1 5 10 15 MKPVTLYDVAEYAGVSYQT lacIq promoter lacI T G C T A C A G C G T C T C A T A C G G LacI-R a t g t g a a a c c a g t a a c g t t a t a c g a t g t c g c a g a g t a t g c c g g t g t c t c t t a t c a g a c c g t a c a c t t t g g t c a t t g c a a t a t g c t a c a g c g t c t c a t a c g g c c a c a g a g a a t a g t c t g g c

4680 20 25 30 35 VSRVVNQASHVSAKTREKVE lacI t t t c c c g c g t g g t g a a c c a g g c c a g c c a c g t t t c t g c g a a a a c g c g g g a a a a a g t g g a a g a a a g g g c g c a c c a c t t g g t c c g g t c g g t g c a a a g a c g c t t t t g c g c c c t t t t t c a c c t t c

4740 40 45 50 55 AAMAELNYIPNRVAQQLAGK lacI MAELNYIPNRVAQQLAGK c g g c g a t g g c g g a g c t g a a t t a c a t t c c c a a c c g c g t g g c a c a a c a a c t g g c g g g c a a a c g c c g c t a c c g c c t c g a c t t a a t g t a a g g g t t g g c g c a c c g t g t t g t t g a c c g c c c g t t t g

4776 60 65 70 QSLLIGVATSSL lacI QSLLIGVATSSL a g t c g t t g c t g a t t g g c g t t g c c a c c t c c a g t c t g g t c a g c a a c g a c t a a c c g c a a c g g t g g a g g t c a g a c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.