Addgene VPS13B^GFP Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: VPS13B^GFP Sequencing Result


Map View

F1ori-F (18,810 .. 14) f1 ori F1ori-R (18,600 .. 18,619) HSV TK poly(A) signal TK-pA-R (18,219 .. 18,238) SacII (18,112) WPRE-R (17,662 .. 17,682) AgeI (17,579) MreI (16,430) SrfI (16,238) BstEII (16,222) PshAI (15,039) BmtI (13,638) NheI (13,634) FspAI (12,560) AflII (12,098) BbvCI (11,199) BspDI * - ClaI * (10,395) KflI (9960) pBABE 3' (133 .. 153) SV40 promoter SV40pro-F (380 .. 399) AvrII (452) EagI (568) Neo-R (588 .. 607) BssHII (1059) RsrII (1178) Neo-F (1198 .. 1217) SV40pA-R (1541 .. 1560) EBV-rev (1595 .. 1614) M13 rev M13 Reverse (1674 .. 1690) lac operator M13/pUC Reverse (1687 .. 1709) lac promoter CAP binding site L4440 (1905 .. 1922) pBR322ori-F (2156 .. 2175) PvuI (3278) Amp-R (3458 .. 3477) AmpR promoter SgrDI (3829) pRS-marker (3875 .. 3894) MluI (4059) SpeI (4080) CMV-F (4600 .. 4620) LNCX (4646 .. 4670) XbaI (4739) AfeI (7756) EGFP-N (8723 .. 8744) EGFP-C (9331 .. 9352) VPS13B^GFP 18,817 bp

Sequence View

5ʹ 3ʹ 60 f1 ori T T G T T C C A A A C T G G F1ori-F t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c

120 f1 ori a t t t t g g g g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g t a a a a c c c c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c

180 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c

240 SV40 promoter g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c

300 SV40 promoter g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g

360 SV40 promoter SV40 ori c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g

420 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c t g a g c t a t t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g a c t c g a t a

AvrII 480 SV40 promoter SV40 ori t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c g g g a g a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g c c c t c

540 1 MI NeoR/KanR MI c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c

EagI 600 5 10 15 20 EQDGLHAGSPAAWVERLFGY NeoR/KanR G A T A A G C C G A T A C Neo-R EQDGLHAGSPAAWVERLFGY a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c

660 25 30 35 40 DWAQQTIGCSDAAVFRLSAQ NeoR/KanR T G A C C C G Neo-R DWAQQTIGCSDAAVFRLSAQ a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c

720 45 50 55 60 GRPVLFVKTDLSGALNELQD NeoR/KanR GRPVLFVKTDLSGALNELQD g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a g g a c g c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t c c t g c

780 65 70 75 80 EAARLSWLATTGVPCAAVLD NeoR/KanR EAARLSWLATTGVPCAAVLD a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c

840 85 90 95 100 VVTEAGRDWLLLGEVPGQDL NeoR/KanR VVTEAGRDWLLLGEVPGQDL t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g

900 105 110 115 120 LSSHLAPAEKVSIMADAMRR NeoR/KanR LSSHLAPAEKVSIMADAMRR t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g

960 125 130 135 140 LHTLDPATCPFDHQAKHRIE NeoR/KanR LHTLDPATCPFDHQAKHRIE t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g

1020 145 150 155 160 RARTRMEAGLVDQDDLDEEH NeoR/KanR RARTRMEAGLVDQDDLDEEH g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g

BssHII 1080 165 170 175 180 QGLAPAELFARLKARMPDGE NeoR/KanR QGLAPAELFARLKARMPDGE a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g c a t g c c c g a c g g c g a g g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c g t a c g g g c t g c c g c t c c

1140 185 190 195 200 DLVVTHGDACLPNIMVENGR NeoR/KanR DLVVTHGDACLPNIMVENGR a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a

RsrII 1200 205 210 215 220 FSGFIDCGRLGVADRYQDIA NeoR/KanR C G T Neo-F FSGFIDCGRLGVADRYQDIA t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a

1260 225 230 235 240 LATRDIAEELGGEWADRFLV NeoR/KanR T G G C T A C C C G T G A T A T T Neo-F LATRDIAEELGGEWADRFLV t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g

1320 245 250 255 260 LYGIAAPDSQRIAFYRLLDE NeoR/KanR LYGIAAPDSQRIAFYRLLDE t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a

1380 265 FF* NeoR/KanR FF* t c t t c t g a g c g g g a c t c t g g g g t t c g c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a a g a a g a c t c g c c c t g a g a c c c c a a g c g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t

1440 t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g

1500 c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g

1560 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g

1620 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t

1680 SV40 poly(A) signal M13 rev C A G T A T C M13 Reverse t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c

1740 -10 M13 rev lac operator lac promoter G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g

1800 -35 lac promoter CAP binding site a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g

1860 t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t

1920 G A G C G A G T G A C T G A G C L4440 c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c

1980 G A L4440 c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c

2040 t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c

2100 ori g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g

2160 ori T T T C T pBR322ori-F g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t

2220 ori A T G G T C C G C A A A G G G pBR322ori-F t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a

2280 ori a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g

2340 ori t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g

2400 ori c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t

2460 ori a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a

2520 ori g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a c a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c c t g t

2580 ori g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a

2640 ori t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a

2700 ori a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a

2760 c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g

2820 a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t

2880 285 280 275 *WHKILSAGIEAIQR AmpR *WHKILSAGIEAIQR a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t

2940 270 265 260 255 NREDMTAQSGTTYIVVIRSP AmpR NREDMTAQSGTTYIVVIRSP t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g

3000 250 245 240 235 KGDPGLAAIIGRSGREGAGS AmpR KGDPGLAAIIGRSGREGAGS t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a

3060 230 225 220 215 KDAIFWGAPLASRLLPGAVK AmpR KDAIFWGAPLASRLLPGAVK t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t

3120 210 205 200 195 DAEMWDILQQRSALTLLEGT AmpR DAEMWDILQQRSALTLLEGT t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a

3180 190 185 180 175 LLKRLTTAMAVPMTTDREDN AmpR LLKRLTTAMAVPMTTDREDN a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a

3240 170 165 160 155 PIAENLEPEWRDLRTVHDGM AmpR PIAENLEPEWRDLRTVHDGM g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c

PvuI 3300 150 145 140 135 NHLFATLEKPGGITTLLLNA AmpR NHLFATLEKPGGITTLLLNA t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g

3360 130 125 120 115 ATNDSMTIAASCLERVTMGD AmpR ATNDSMTIAASCLERVTMGD g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g

3420 110 105 100 95 TLHKETVPSYEVLDNQSYHI AmpR TLHKETVPSYEVLDNQSYHI g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c

3480 90 85 80 75 RRGLQEQGADIRSLVAGCLL AmpR A T A A T A C C G C G C C A C A T A G C Amp-R RRGLQEQGADIRSLVAGCLL c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t

3540 70 65 60 55 VKFTSMMPFREEPRFSELIK AmpR VKFTSMMPFREEPRFSELIK a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t

3600 50 45 40 35 GSNLDLEIYGVRAGLQDEAD AmpR GSNLDLEIYGVRAGLQDEAD c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a

3660 30 25 KVKVLTEPH 20 15 signal sequence AFVPLCFAAFF AmpR KVKVLTEPHAFVPLCFAAFF t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c

3720 10 5 1 signal sequence PILAVRFHQISM AmpR AmpR promoter PILAVRFHQISM g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t

3780 AmpR promoter a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a

SgrDI 3840 AmpR promoter a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c g a c g g a t c g t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g c t g c c t a g c

3900 A T G T T A G A C G A G A C T A C G G C pRS-marker g g a g a t c t c c c g a t c c c c t a t g g t c g a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t c c t c t a g a g g g c t a g g g g a t a c c a g c t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a

3960 t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t

4020 t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t

MluI 4080 CMV enhancer g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t

SpeI 4140 CMV enhancer c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g

4200 CMV enhancer g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a

4260 CMV enhancer t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g

4320 CMV enhancer a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g

4380 CMV enhancer c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a

4440 CMV enhancer a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t

4500 CMV enhancer CMV promoter c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c

4560 CMV promoter g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g

4620 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c

4680 CMV promoter A G C T C G T T T A G T G A A C C G T C A G A T C LNCX t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c g c c t g g a g a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g c g g a c c t c t g

XbaI 4740 g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g g g a c c g a t c c a g c c t c c g g a c t c c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c c c t g g c t a g g t c g g a g g c c t g a g

4800 MLESYVTPILMSYVNRY t a g a g c c a c c a t g c t c g a g t c t t a c g t t a c a c c c a t c c t c a t g a g t t a c g t a a a t a g a t a a t c t c g g t g g t a c g a g c t c a g a a t g c a a t g t g g g t a g g a g t a c t c a a t g c a t t t a t c t a t

4860 IKNLKPSDLQLSLWGGDVVL c a t c a a a a a t c t g a a g c c t t c t g a c t t a c a g c t g a g t t t g t g g g g c g g c g a c g t g g t c c t g t a g t t t t t a g a c t t c g g a a g a c t g a a t g t c g a c t c a a a c a c c c c g c c g c t g c a c c a g g a

4920 SKLELKLDVLEQELKLPFTF g t c g a a g c t a g a a t t g a a g c t t g a c g t g c t a g a a c a g g a a c t g a a a c t c c c c t t c a c c t t c a g c t t c g a t c t t a a c t t c g a a c t g c a c g a t c t t g t c c t t g a c t t t g a g g g g a a g t g g a a

4980 LSGHIHELRIHVPWTKLGSE c t t g t c g g g c c a c a t c c a c g a g c t c a g g a t a c a t g t g c c a t g g a c c a a g c t g g g t a g c g a g a a c a g c c c g g t g t a g g t g c t c g a g t c c t a t g t a c a c g g t a c c t g g t t c g a c c c a t c g c t

5040 PVVITINTMECILKLKDGIQ g c c t g t g g t c a t t a c a a t c a a c a c t a t g g a a t g t a t c c t c a a g c t g a a g g a t g g c a t c c a c g g a c a c c a g t a a t g t t a g t t g t g a t a c c t t a c a t a g g a g t t c g a c t t c c t a c c g t a g g t

5100 DDHESCGSNSTNRSTAESTK a g a c g a c c a c g a g a g c t g c g g c a g t a a c t c t a c c a a c a g a a g c a c a g c c g a g t c g a c c a a t c t g c t g g t g c t c t c g a c g c c g t c a t t g a g a t g g t t g t c t t c g t g t c g g c t c a g c t g g t t

5160 SSIKPRRMQQAAPTDPDLPP g a g c t c t a t c a a g c c a c g g c g g a t g c a g c a g g c c g c c c c c a c t g a c c c c g a c c t c c c g c c c t c g a g a t a g t t c g g t g c c g c c t a c g t c g t c c g g c g g g g g t g a c t g g g g c t g g a g g g c g g

5220 GYVQSLIRRVVNNVNIVINN t g g t t a t g t a c a g t c c c t g a t c a g a c g a g t g g t g a a c a a c g t g a a c a t c g t g a t c a a c a a a c c a a t a c a t g t c a g g g a c t a g t c t g c t c a c c a c t t g t t g c a c t t g t a g c a c t a g t t g t t

5280 LILKYVEDDIVLSVNITSAE c c t a a t c c t g a a g t a c g t g g a a g a c g a c a t t g t t c t g t c t g t g a a c a t c a c a t c t g c c g a g g a t t a g g a c t t c a t g c a c c t t c t g c t g t a a c a a g a c a g a c a c t t g t a g t g t a g a c g g c t

5340 CYTVGELWDRAFMDISATDL a t g c t a c a c c g t g g g g g a a c t g t g g g a c c g g g c t t t c a t g g a c a t a a g t g c c a c a g a c c t t a c g a t g t g g c a c c c c c t t g a c a c c c t g g c c c g a a a g t a c c t g t a t t c a c g g t g t c t g g a

5400 VLRKVINFSDCTVCLDKRNA g g t g c t c c g c a a g g t g a t a a a t t t c a g c g a c t g c a c c g t g t g t c t t g a t a a g a g a a a t g c c c a c g a g g c g t t c c a c t a t t t a a a g t c g c t g a c g t g g c a c a c a g a a c t a t t c t c t t t a c g

5460 SGKIEFYQDPLLYKCSFRTR c t c t g g c a a a a t c g a g t t c t a c c a a g a t c c c c t c c t g t a c a a g t g c t c t t t c a g a a c t a g g a g a c c g t t t t a g c t c a a g a t g g t t c t a g g g g a g g a c a t g t t c a c g a g a a a g t c t t g a t c

5520 LHFTYENLNSKMPSVIKIHT a c t a c a c t t c a c c t a c g a g a a t t t g a a c a g c a a a a t g c c c t c a g t c a t t a a g a t c c a c a c t g a t g t g a a g t g g a t g c t c t t a a a c t t g t c g t t t t a c g g g a g t c a g t a a t t c t a g g t g t g

5580 LVESLKLSITDQQLPMFIRI a c t g g t g g a a t c g c t a a a g c t g a g t a t c a c c g a c c a a c a a t t a c c t a t g t t t a t c a g g a t t g a c c a c c t t a g c g a t t t c g a c t c a t a g t g g c t g g t t g t t a a t g g a t a c a a a t a g t c c t a

5640 MQLGIALYYGEIGNFKEGEI c a t g c a g c t g g g a a t c g c c c t g t a c t a c g g t g a g a t c g g c a a c t t c a a g g a g g g c g a g a t g t a c g t c g a c c c t t a g c g g g a c a t g a t g c c a c t c t a g c c g t t g a a g t t c c t c c c g c t c t a

5700 EDLTCHNKDMLGNITGSEDE c g a g g a c c t g a c a t g t c a c a a c a a g g a t a t g c t g g g c a a c a t c a c c g g a t c t g a g g a c g a g c t c c t g g a c t g t a c a g t g t t g t t c c t a t a c g a c c c g t t g t a g t g g c c t a g a c t c c t g c t

5760 TRIDMQYPAQHKGQELYSQQ g a c t c g g a t c g a c a t g c a g t a c c c c g c c c a g c a c a a g g g c c a g g a a t t g t a c t c t c a a c a c t g a g c c t a g c t g t a c g t c a t g g g g c g g g t c g t g t t c c c g g t c c t t a a c a t g a g a g t t g t

5820 DEEQPQGWVSWAWSFVPAIV g g a t g a a g a g c a a c c g c a g g g a t g g g t t t c c t g g g c t t g g t c a t t c g t g c c c g c c a t c g t c c t a c t t c t c g t t g g c g t c c c t a c c c a a a g g a c c c g a a c c a g t a a g c a c g g g c g g t a g c a

5880 SYDDGEEDFVGNDPASTMHQ c t c t t a t g a t g a c g g a g a g g a a g a c t t c g t c g g a a a t g a c c c t g c c t c a a c c a t g c a c c a g a g a a t a c t a c t g c c t c t c c t t c t g a a g c a g c c t t t a c t g g g a c g g a g t t g g t a c g t g g t

5940 QKAQTLKDPIVSIGFYCTKA g c a g a a a g c c c a g a c c c t g a a g g a c c c t a t t g t g t c c a t a g g c t t t t a c t g t a c c a a a g c c g t c t t t c g g g t c t g g g a c t t c c t g g g a t a a c a c a g g t a t c c g a a a a t g a c a t g g t t t c g

6000 TVTFKLTEMQVESSYYSPQK c a c c g t c a c c t t c a a g t t a a c g g a g a t g c a a g t g g a a t c t a g t t a c t a t a g c c c t c a g a a g t g g c a g t g g a a g t t c a a t t g c c t c t a c g t t c a c c t t a g a t c a a t g a t a t c g g g a g t c t t

6060 VKSKEVLCWEQEGTTVEALM g g t g a a a a g c a a g g a a g t a t t a t g t t g g g a a c a g g a a g g c a c c a c c g t a g a g g c c c t c a t c c a c t t t t c g t t c c t t c a t a a t a c a a c c c t t g t c c t t c c g t g g t g g c a t c t c c g g g a g t a

6120 MGEPFFDCQIGFVGCRAMCL g a t g g g c g a g c c c t t t t t c g a t t g t c a a a t c g g c t t c g t g g g a t g c a g g g c c a t g t g c c t c t a c c c g c t c g g g a a a a a g c t a a c a g t t t a g c c g a a g c a c c c t a c g t c c c g g t a c a c g g a

6180 KGIMGVKDFEENMNRSETEA a a a g g g c a t c a t g g g c g t g a a a g a c t t t g a a g a g a a c a t g a a t a g a t c a g a a a c a g a a g c t t t c c c g t a g t a c c c g c a c t t t c t g a a a c t t c t c t t g t a c t t a t c t a g t c t t t g t c t t c g

6240 CFFICGDNLSTKGFTYLTNS c t g c t t c t t c a t t t g c g g c g a t a a t t t g t c c a c a a a a g g c t t t a c a t a c c t g a c c a a c t c g a c g a a g a a g t a a a c g c c g c t a t t a a a c a g g t g t t t t c c g a a a t g t a t g g a c t g g t t g a g

6300 LFDYRSPENNGTRAEFILDS c c t c t t t g a c t a t a g a a g c c c t g a g a a c a a c g g c a c c a g a g c t g a g t t c a t a c t c g a t a g g g a g a a a c t g a t a t c t t c g g g a c t c t t g t t g c c g t g g t c t c g a c t c a a g t a t g a g c t a t c

6360 THHKETYTEIAGMQRFGAFY t a c c c a t c a c a a a g a g a c a t a c a c c g a a a t c g c c g g c a t g c a g c g g t t c g g c g c c t t t t a a t g g g t a g t g t t t c t c t g t a t g t g g c t t t a g c g g c c g t a c g t c g c c a a g c c g c g g a a a a t

6420 MDYLYTMENTSGKGSTNQQD c a t g g a c t a c c t g t a c a c c a t g g a a a a c a c c a g c g g a a a a g g t a g c a c a a a c c a g c a g g a g t a c c t g a t g g a c a t g t g g t a c c t t t t g t g g t c g c c t t t t c c a t c g t g t t t g g t c g t c c t

6480 FSSGKSEDLGTVQEKSTKSL c t t t a g c t c c g g a a a g t c c g a a g a c c t c g g t a c a g t c c a g g a g a a g a g c a c a a a g a g c c t g a a a t c g a g g c c t t t c a g g c t t c t g g a g c c a t g t c a g g t c c t c t t c t c g t g t t t c t c g g a

6540 VIGPLDFRLDSSAVHRILKM a g t a a t c g g c c c t c t g g a c t t c c g g c t c g a c t c c t c t g c c g t g c a c a g a a t c c t g a a a a t t c a t t a g c c g g g a g a c c t g a a g g c c g a g c t g a g g a g a c g g c a c g t g t c t t a g g a c t t t t a

6600 IVCALEHEYEPYSRLKSDIK g a t c g t c t g t g c c c t g g a g c a c g a g t a c g a a c c t t a c t c t c g g c t t a a a a g c g a c a t t a a c t a g c a g a c a c g g g a c c t c g t g c t c a t g c t t g g a a t g a g a g c c g a a t t t t c g c t g t a a t t

6660 DENETILNPEEVALLEEYIP g g a c g a g a a c g a g a c c a t c c t g a a c c c t g a g g a g g t g g c g c t g t t a g a a g a g t a c a t c c c c c t g c t c t t g c t c t g g t a g g a c t t g g g a c t c c t c c a c c g c g a c a a t c t t c t c a t g t a g g g

6720 TRHTSVTLLKCTCTISMAEF t a c c a g a c a c a c t t c t g t g a c a c t a c t g a a g t g c a c c t g t a c a a t c a g t a t g g c t g a a t t a t g g t c t g t g t g a a g a c a c t g t g a t g a c t t c a c g t g g a c a t g t t a g t c a t a c c g a c t t a a

6780 NLLDHLLPVIMGEKNSSNFM c a a t c t g c t g g a c c a c c t g c t g c c c g t c a t c a t g g g c g a g a a g a a c t c c t c c a a t t t c a t g t t a g a c g a c c t g g t g g a c g a c g g g c a g t a g t a c c c g c t c t t c t t g a g g a g g t t a a a g t a

6840 NTTNFQSLRPLPSIRILVDK g a a t a c t a c c a a t t t c c a g a g c c t g a g a c c c c t t c c t a g c a t c a g a a t t c t a g t t g a t a a c t t a t g a t g g t t a a a g g t c t c g g a c t c t g g g g a a g g a t c g t a g t c t t a a g a t c a a c t a t t

6900 INLEHSVPMYAEQLVHVVSS g a t c a a c c t a g a g c a c t c t g t g c c a a t g t a c g c c g a g c a g c t t g t c c a c g t g g t a t c t t c c t a g t t g g a t c t c g t g a g a c a c g g t t a c a t g c g g c t c g t c g a a c a g g t g c a c c a t a g a a g

6960 LTQPSDNLLHYCYVHCYLKI a c t c a c c c a g c c t t c a g a t a a c c t g c t g c a t t a c t g t t a c g t t c a c t g c t a c c t g a a g a t t g a g t g g g t c g g a a g t c t a t t g g a c g a c g t a a t g a c a a t g c a a g t g a c g a t g g a c t t c t a

7020 FGFQAGLTSLDCSGSYCLPV c t t c g g c t t c c a a g c a g g c c t g a c a t c g c t c g a c t g c t c t g g a t c a t a c t g c c t c c c a g t g a a g c c g a a g g t t c g t c c g g a c t g t a g c g a g c t g a c g a g a c c t a g t a t g a c g g a g g g t c a

7080 PVIPSFSTALYGKLLKLPTC c c c c g t g a t c c c c a g t t t t t c g a c c g c t c t g t a c g g a a a g t t a c t g a a g c t g c c a a c c t g g g g g c a c t a g g g g t c a a a a a g c t g g c g a g a c a t g c c t t t c a a t g a c t t c g a c g g t t g g a c

7140 WTKRSQIAITEGIFELPNLT t t g g a c c a a g c g t a g t c a g a t c g c c a t c a c c g a a g g a a t c t t c g a g c t g c c t a a c c t g a c a a c c t g g t t c g c a t c a g t c t a g c g g t a g t g g c t t c c t t a g a a g c t c g a c g g a t t g g a c t g

7200 IQATRAQTLLLQAIYQSWSH a a t c c a g g c c a c c a g a g c t c a g a c g c t g t t g c t c c a a g c c a t c t a c c a g t c t t g g t c c c a t t a g g t c c g g t g g t c t c g a g t c t g c g a c a a c g a g g t t c g g t a g a t g g t c a g a a c c a g g g t

7260 LGNVSSSAVIEALINEIFLS c c t g g g c a a c g t g a g c t c c t c t g c c g t g a t c g a g g c c c t g a t c a a c g a g a t c t t t t t g a g g g a c c c g t t g c a c t c g a g g a g a c g g c a c t a g c t c c g g g a c t a g t t g c t c t a g a a a a a c t c

7320 IGVKSKNPLPTLEGSIQNVE t a t c g g c g t g a a a a g c a a g a a c c c t c t g c c t a c a c t g g a a g g g a g c a t c c a g a a c g t g g a a t a g c c g c a c t t t t c g t t c t t g g g a g a c g g a t g t g a c c t t c c c t c g t a g g t c t t g c a c c t

7380 LKYCSTSLVKCASGTMGSIK g c t g a a a t a c t g t t c a a c a a g c c t t g t g a a a t g c g c c a g c g g g a c c a t g g g a a g c a t c a a c g a c t t t a t g a c a a g t t g t t c g g a a c a c t t t a c g c g g t c g c c c t g g t a c c c t t c g t a g t t

7440 ICAKAPVDSGKEKLIPLLQG g a t t t g c g c t a a a g c t c c g g t g g a c a g c g g c a a g g a a a a a c t g a t a c c a c t g c t g c a g g g c t a a a c g c g a t t t c g a g g c c a c c t g t c g c c g t t c c t t t t t g a c t a t g g t g a c g a c g t c c c

7500 PSDTKDLHSTKWLNESRKPE c c c g a g c g a c a c c a a a g a c c t c c a c a g t a c t a a g t g g c t g a a t g a g a g c c g a a a a c c t g a g g g c t c g c t g t g g t t t c t g g a g g t g t c a t g a t t c a c c g a c t t a c t c t c g g c t t t t g g a c t

7560 SLLAPDLMAFTIQVPQYIDY g a g t c t c c t t g c c c c c g a t c t g a t g g c c t t c a c c a t c c a g g t g c c c c a a t a c a t c g a c t a c t c a g a g g a a c g g g g g c t a g a c t a c c g g a a g t g g t a g g t c c a c g g g g t t a t g t a g c t g a t

7620 CHNSGAVLLCSIQGLAVNID c t g c c a c a a t t c a g g g g c t g t g t t a c t g t g c a g c a t t c a g g g a c t g g c t g t g a a c a t c g a g a c g g t g t t a a g t c c c c g a c a c a a t g a c a c g t c g t a a g t c c c t g a c c g a c a c t t g t a g c t

7680 PILYTWLIYQPQKRTSRHMQ c c c c a t t c t g t a c a c c t g g c t c a t c t a t c a g c c a c a a a a g a g a a c c a g t c g t c a c a t g c a g g g g t a a g a c a t g t g g a c c g a g t a g a t a g t c g g t g t t t t c t c t t g g t c a g c a g t g t a c g t

7740 QQPVVAVPLVMPVCRRKEDE g c a g c a g c c c g t g g t a g c c g t g c c t c t c g t g a t g c c a g t g t g t c g c c g g a a a g a g g a c g a c g t c g t c g g g c a c c a t c g g c a c g g a g a g c a c t a c g g t c a c a c a g c g g c c t t t c t c c t g c t

AfeI 7800 VSIGSAPLAKQQSYQASEYA g g t g t c t a t c g g c a g c g c t c c t c t c g c a a a g c a g c a g t c t t a c c a g g c c t c g g a g t a c g c c c a c a g a t a g c c g t c g c g a g g a g a g c g t t t c g t c g t c a g a a t g g t c c g g a g c c t c a t g c g

7860 SSPVKTKTVTESRPLSVPVK c t c t a g c c c t g t g a a g a c c a a g a c c g t c a c t g a a t c t c g a c c c c t t t c g g t c c c c g t g a a g a g a t c g g g a c a c t t c t g g t t c t g g c a g t g a c t t a g a g c t g g g g a a a g c c a g g g g c a c t t

7920 AMLNISESCRSPEERMKEFI a g c c a t g c t t a a c a t c t c t g a g t c c t g c a g a a g t c c g g a g g a g c g g a t g a a g g a a t t c a t t c g g t a c g a a t t g t a g a g a c t c a g g a c g t c t t c a g g c c t c c t c g c c t a c t t c c t t a a g t a

7980 GIVWNAVKHLTLQLEVQSCC c g g t a t t g t g t g g a a t g c c g t g a a a c a t c t c a c a c t g c a a c t c g a g g t g c a a a g c t g c t g g c c a t a a c a c a c c t t a c g g c a c t t t g t a g a g t g t g a c g t t g a g c t c c a c g t t t c g a c g a c

8040 VFIPNDSLPSPSTIVSGDIP t g t g t t c a t c c c c a a c g a c t c t t t g c c t a g c c c t t c c a c c a t c g t g t c t g g c g a t a t c c c a c a c a a g t a g g g g t t g c t g a g a a a c g g a t c g g g a a g g t g g t a g c a c a g a c c g c t a t a g g g

8100 GTVRSWYHGQTSMPGTLVLC g g g g a c t g t c c g g a g c t g g t a c c a c g g c c a g a c c a g t a t g c c c g g c a c t c t a g t a c t c t g c c c c t g a c a g g c c t c g a c c a t g g t g c c g g t c t g g t c a t a c g g g c c g t g a g a t c a t g a g a c

8160 LPQIKIISAGHKYMEPLQEI t c t t c c a c a g a t t a a a a t t a t c t c t g c c g g a c a c a a g t a c a t g g a a c c t c t c c a g g a a a t a g a a g g t g t c t a a t t t t a a t a g a g a c g g c c t g t g t t c a t g t a c c t t g g a g a g g t c c t t t a

8220 PFVIPRPILEEGDAFPWTIS c c c a t t c g t g a t c c c t a g a c c t a t c c t g g a a g a a g g c g a t g c g t t c c c t t g g a c a a t c t c g g g t a a g c a c t a g g g a t c t g g a t a g g a c c t t c t t c c g c t a c g c a a g g g a a c c t g t t a g a g

8280 LHNFSIYTLLGKQVTLCLVE c c t c c a t a a t t t t t c g a t c t a t a c a c t g c t g g g a a a g c a g g t t a c g c t g t g c c t t g t g g a g g a g g t a t t a a a a a g c t a g a t a t g t g a c g a c c c t t t c g t c c a a t g c g a c a c g g a a c a c c t

8340 PMGCTSTLAVTSQKLLATGP g c c a a t g g g t t g c a c a t c t a c c c t g g c c g t g a c c t c g c a g a a a c t g c t t g c c a c c g g a c c c g g t t a c c c a a c g t g t a g a t g g g a c c g g c a c t g g a g c g t c t t t g a c g a a c g g t g g c c t g g

8400 DTRHSFVVCLHVDLESLEIK a g a c a c c c g g c a c t c a t t c g t a g t c t g c t t g c a t g t g g a c t t a g a g t c c c t c g a a a t c a a t c t g t g g g c c g t g a g t a a g c a t c a g a c g a a c g t a c a c c t g a a t c t c a g g g a g c t t t a g t t

8460 CSNPQVQLFYELTDIMNKVW a t g c t c t a a c c c g c a g g t g c a g c t g t t t t a c g a a c t g a c c g a t a t t a t g a a c a a g g t g t g t a c g a g a t t g g g c g t c c a c g t c g a c a a a a t g c t t g a c t g g c t a t a a t a c t t g t t c c a c a c

8520 NKIQKRGNLNLSPTSPETMA g a a c a a a a t a c a g a a a c g g g g c a a c c t t a a c c t g a g c c c a a c t a g c c c a g a g a c a a t g g c c t t g t t t t a t g t c t t t g c c c c g t t g g a a t t g g a c t c g g g t t g a t c g g g t c t c t g t t a c c g

8580 GPVPTSPVRSSIGTAPPDTS t g g g c c t g t g c c t a c g a g t c c c g t g a g a t c c a g c a t c g g t a c c g c c c c t c c t g a c a c t t c a c c c g g a c a c g g a t g c t c a g g g c a c t c t a g g t c g t a g c c a t g g c g g g g a g g a c t g t g a a g

8640 TCSPSADIGTTTEGDSIQAG t a c c t g t t c g c c t t c c g c t g a c a t c g g c a c a a c t a c c g a g g g g g a c a g t a t a c a g g c t g g a t g g a c a a g c g g a a g g c g a c t g t a g c c g t g t t g a t g g c t c c c c c t g t c a t a t g t c c g a c c

8700 1 M 1a V 5 SKGEEL EGFP 1 M 1a V 5 SKGEEL EGFP EESPGSGSSGSGMVSKGEEL *SGAHHALALLQ c g a g g a g t c t c c t g g c t c t g g c t c t t c a g g a t c c g g c a t g g t g a g c a a g g g c g a g g a g c t g c t c c t c a g a g g a c c g a g a c c g a g a a g t c c t a g g c c g t a c c a c t c g t t c c c g c t c c t c g a

8760 10 15 20 25 FTGVVPILVELDGDVNGHKF EGFP 10 15 20 25 FTGVVPILVELDGDVNGHKF EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N FTGVVPILVELDGDVNGHKF EGPHHGDQDLQVAVYVAVLE g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a

8820 30 35 40 45 SVSGEGEGDATYGKLTLKFI EGFP 30 35 40 45 SVSGEGEGDATYGKLTLKFI EGFP SVSGEGEGDATYGKLTLKFI AHGALALAIGGVALQGQLED c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a

8880 50 55 60 65 CTTGKLPVPWPTLVTTLTYG EGFP 50 55 60 65 CTTGKLPVPWPTLVTTLTYG EGFP CTTGKLPVPWPTLVTTLTYG AGGALQGHGPGGEHGGQGVA c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c

8940 70 75 80 85 VQCFSRYPDHMKQHDFFKSA EGFP 70 75 80 85 VQCFSRYPDHMKQHDFFKSA EGFP VQCFSRYPDHMKQHDFFKSA HLAEAAVGVVHLLVVEELGG c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g

9000 90 95 100 105 MPEGYVQERTIFFKDDGNYK EGFP 90 95 100 105 MPEGYVQERTIFFKDDGNYK EGFP MPEGYVQERTIFFKDDGNYK HGFAVDLLAGDEELVVAVVL c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t

9060 110 115 120 125 TRAEVKFEGDTLVNRIELKG EGFP 110 115 120 125 TRAEVKFEGDTLVNRIELKG EGFP TRAEVKFEGDTLVNRIELKG GAGLHLELAVGQHVADLQLA g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c

9120 130 135 140 145 IDFKEDGNILGHKLEYNYNS EGFP 130 135 140 145 IDFKEDGNILGHKLEYNYNS EGFP IDFKEDGNILGHKLEYNYNS DVELLVAVDQPVLQLVVVVA c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c

9180 150 155 160 165 HNVYIMADKQKNGIKVNFKI EGFP 150 155 160 165 HNVYIMADKQKNGIKVNFKI EGFP HNVYIMADKQKNGIKVNFKI VVDIDHGVLLLVADLHVELD c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a

9240 170 175 180 185 RHNIEDGSVQLADHYQQNTP EGFP 170 175 180 185 RHNIEDGSVQLADHYQQNTP EGFP RHNIEDGSVQLADHYQQNTP AVVDLVAAHLEGVVVLLVGG c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g

9300 190 195 200 205 IGDGPVLLPDNHYLSTQSAL EGFP 190 195 200 205 IGDGPVLLPDNHYLSTQSAL EGFP IGDGPVLLPDNHYLSTQSAL DAVAGHQQGVVVVQAGLGGQ c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a

9360 210 215 220 225 SKDPNEKRDHMVLLEFVTAA EGFP 210 215 220 225 SKDPNEKRDHMVLLEFVTAA EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C SKDPNEKRDHMVLLEFVTAA AFVGVLLAIVHDQQLEHGGG g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g

9420 230 235 GITLGMDELYK (in frame with EGFP) GGGSGGFSD EGFP 230 235 GITLGMDELYK (in frame with EGFP) GGGSGGFSD EGFP GITLGMDELYKGGGSGGFSD PDSEAHVLQVLAASGASETI c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g g c g g c g g a t c c g g c g g a t t c a g t g a g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c c c g c c g c c t a g g c c g c c t a a g t c a c t

9480 (in frame with EGFP) SVTLEQTTSNIGGTSGRVSL (in frame with EGFP) SVTLEQTTSNIGGTSGRVSL SVTLEQTTSNIGGTSGRVSL AHGKFLGSGIDTTGTAPHRQ t a g c g t g a c c c t t g a a c a g a c c a c t t c c a a t a t c g g t g g t a c c a g t g g c c g g g t g t c t c t a t c g c a c t g g g a a c t t g t c t g g t g a a g g t t a t a g c c a c c a t g g t c a c c g g c c c a c a g a g a

9540 (in frame with EGFP) WMQWVLPKITIKLFAPDPEN (in frame with EGFP) WMQWVLPKITIKLFAPDPEN WMQWVLPKITIKLFAPDPEN PHLPDQRFDCNLQEGRVGLI g t g g a t g c a g t g g g t c t t g c c t a a a a t c a c a a t t a a g c t g t t c g c c c c t g a c c c c g a g a a c a c c t a c g t c a c c c a g a a c g g a t t t t a g t g t t a a t t c g a c a a g c g g g g a c t g g g g c t c t t

9600 (in frame with EGFP) KGTEVCMVSELEDLSASIDV (in frame with EGFP) KGTEVCMVSELEDLSASIDV KGTEVCMVSELEDLSASIDV LTGFHAHHRFQLVQGGANVH t a a g g g t a c c g a a g t g t g c a t g g t g t c t g a a c t g g a g g a c c t g t c c g c c a g c a t t g a c g t a t t c c c a t g g c t t c a c a c g t a c c a c a g a c t t g a c c t c c t g g a c a g g c g g t c g t a a c t g c a

9660 (in frame with EGFP) QDVYTKVKCKIESFNIDHYR (in frame with EGFP) QDVYTKVKCKIESFNIDHYR QDVYTKVKCKIESFNIDHYR LVHVGLDLALDLGKVYVVVA g c a g g a c g t g t a c a c c a a g g t c a a g t g c a a g a t c g a g t c c t t t a a c a t a g a c c a c t a c c g c g t c c t g c a c a t g t g g t t c c a g t t c a c g t t c t a g c t c a g g a a a t t g t a t c t g g t g a t g g c

9720 (in frame with EGFP) SSLGEECWSLGQCGGVFLSC (in frame with EGFP) SSLGEECWSLGQCGGVFLSC SSLGEECWSLGQCGGVFLSC RRQPLFAPGQALTAAHKKAA c t c t t c t c t g g g g g a g g a a t g c t g g t c c c t g g g c c a g t g t g g c g g c g t g t t t c t t a g c t g g a g a a g a g a c c c c c t c c t t a c g a c c a g g g a c c c g g t c a c a c c g c c g c a c a a a g a a t c g a c

9780 (in frame with EGFP) TDKLNRRTLLVRPISKQDPF (in frame with EGFP) TDKLNRRTLLVRPISKQDPF TDKLNRRTLLVRPISKQDPF GILEVSAGEKHSRDGLLVGE c a c c g a t a a g c t c a a c a g a c g c a c c c t c c t t g t g a g a c c t a t c t c c a a g c a a g a c c c c t t g t g g c t a t t c g a g t t g t c t g c g t g g g a g g a a c a c t c t g g a t a g a g g t t c g t t c t g g g g a a

9840 (in frame with EGFP) SNCSGFFPSTTTKLLDGTHQ (in frame with EGFP) SNCSGFFPSTTTKLLDGTHQ SNCSGFFPSTTTKLLDGTHQ TVAGSKKRAGGCLQQVSGM c a g t a a c t g c t c c g g a t t t t t t c c t a g c a c c a c c a c a a a g c t g c t g g a c g g a a c c c a t c a g t c a t t g a c g a g g c c t a a a a a a g g a t c g t g g t g g t g t t t c g a c g a c c t g c c t t g g g t a g t

9900 (in frame with EGFP) QHGFLSLTYTKAVTKNVRHK (in frame with EGFP) QHGFLSLTYTKAVTKNVRHK QHGFLSLTYTKAVTKNVRHK g c a a c a c g g c t t c t t a t c t c t g a c c t a c a c c a a g g c t g t c a c c a a g a a c g t g a g a c a t a a c g t t g t g c c g a a g a a t a g a g a c t g g a t g t g g t t c c g a c a g t g g t t c t t g c a c t c t g t a t t

9960 (in frame with EGFP) LTSRNERRSFHKLSEGLMDG (in frame with EGFP) LTSRNERRSFHKLSEGLMDG LTSRNERRSFHKLSEGLMDG g c t g a c c a g c c g g a a t g a g a g a a g a t c t t t c c a c a a g c t a t c c g a a g g g c t c a t g g a c g g c g a c t g g t c g g c c t t a c t c t c t t c t a g a a a g g t g t t c g a t a g g c t t c c c g a g t a c c t g c c

KflI 10,020 (in frame with EGFP) SPHFLHEILLSAQAFDIVLY (in frame with EGFP) SPHFLHEILLSAQAFDIVLY SPHFLHEILLSAQAFDIVLY g t c c c c c c a c t t c c t g c a c g a a a t a c t g c t c t c c g c c c a a g c c t t c g a c a t a g t t c t g t a c a g g g g g g t g a a g g a c g t g c t t t a t g a c g a g a g g c g g g t t c g g a a g c t g t a t c a a g a c a t

10,080 (in frame with EGFP) FPLLNAIASIFQAKLPKTQK (in frame with EGFP) FPLLNAIASIFQAKLPKTQK FPLLNAIASIFQAKLPKTQK c t t t c c c c t a c t g a a c g c c a t c g c c a g c a t c t t c c a g g c c a a g c t g c c t a a g a c a c a a a a g a a a g g g g a t g a c t t g c g g t a g c g g t c g t a g a a g g t c c g g t t c g a c g g a t t c t g t g t t t t

10,140 (in frame with EGFP) EKRKSPGQPMRTHTLTSRNL (in frame with EGFP) EKRKSPGQPMRTHTLTSRNL EKRKSPGQPMRTHTLTSRNL g g a a a a g c g g a a g a g c c c c g g a c a g c c a a t g c g g a c t c a c a c a c t c a c c a g c a g a a a c c t c c t t t t c g c c t t c t c g g g g c c t g t c g g t t a c g c c t g a g t g t g t g a g t g g t c g t c t t t g g a

10,200 (in frame with EGFP) PLIYVNTSVIRIFIPKTEEM (in frame with EGFP) PLIYVNTSVIRIFIPKTEEM PLIYVNTSVIRIFIPKTEEM g c c c c t c a t c t a c g t g a a t a c t t c g g t g a t c a g a a t c t t t a t c c c c a a g a c a g a a g a g a t c g g g g a g t a g a t g c a c t t a t g a a g c c a c t a g t c t t a g a a a t a g g g g t t c t g t c t t c t c t a

10,260 (in frame with EGFP) QPTVEANQAAKEDTVVLKIG (in frame with EGFP) QPTVEANQAAKEDTVVLKIG QPTVEANQAAKEDTVVLKIG g c a g c c g a c c g t g g a a g c c a a c c a g g c c g c c a a a g a a g a t a c a g t g g t t c t a a a a a t c g g c g t c g g c t g g c a c c t t c g g t t g g t c c g g c g g t t t c t t c t a t g t c a c c a a g a t t t t t a g c c

10,320 (in frame with EGFP) SVAMAPQADNPLGRSVLRKD (in frame with EGFP) SVAMAPQADNPLGRSVLRKD SVAMAPQADNPLGRSVLRKD c t c t g t t g c a a t g g c c c c g c a g g c c g a c a a c c c t c t g g g a a g a a g c g t g c t g a g a a a g g a g a g a c a a c g t t a c c g g g g c g t c c g g c t g t t g g g a g a c c c t t c t t c g c a c g a c t c t t t c c t

10,380 (in frame with EGFP) IYQRALNLGILRDPGSEIED (in frame with EGFP) IYQRALNLGILRDPGSEIED IYQRALNLGILRDPGSEIED *RARFRPMSLSGPLSISS t a t c t a t c a g c g g g c c c t g a a c c t g g g c a t a c t g a g a g a c c c t g g a a g c g a a a t c g a a g a a t a g a t a g t c g c c c g g g a c t t g g a c c c g t a t g a c t c t c t g g g a c c t t c g c t t t a g c t t c t

BspDI*ClaI* 10,440 (in frame with EGFP) RQYQIDLQSINIGTAQWHQL (in frame with EGFP) RQYQIDLQSINIGTAQWHQL RQYQIDLQSINIGTAQWHQL RCYWISRCLMLMPVAWHCWS c c g a c a g t a c c a g a t c g a t c t g c a g a g c a t c a a c a t c g g c a c g g c c c a a t g g c a c c a g c t g g c t g t c a t g g t c t a g c t a g a c g t c t c g t a g t t g t a g c c g t g c c g g g t t a c c g t g g t c g a

10,500 (in frame with EGFP) KPEKESVSGGVVTETERNSQ (in frame with EGFP) KPEKESVSGGVVTETERNSQ KPEKESVSGGVVTETERNSQ FGSFSDTDPPTTVSVSLFEW g a a g c c t g a g a a g g a g t c t g t g t c c g g g g g c g t c g t t a c a g a g a c c g a a a g a a a c t c c c a c t t c g g a c t c t t c c t c a g a c a c a g g c c c c c g c a g c a a t g t c t c t g g c t t t c t t t g a g g g t

10,560 (in frame with EGFP) NPALEWNMASSIRRHQERRA (in frame with EGFP) NPALEWNMASSIRRHQERRA NPALEWNMASSIRRHQERRA FGARSHFMALLMLRCWSLLA a a a c c c c g c c c t g g a g t g g a a c a t g g c g a g c a g c a t a a g g c g g c a c c a g g a g a g a a g a g c t t t g g g g c g g g a c c t c a c c t t g t a c c g c t c g t c g t a t t c c g c c g t g g t c c t c t c t t c t c g

10,620 (in frame with EGFP) ILTPVLTDFSVRITGAPAVI (in frame with EGFP) ILTPVLTDFSVRITGAPAVI ILTPVLTDFSVRITGAPAVI MRVGTSVSKETLIVPAGATI c a t c c t g a c c c c t g t g c t c a c a g a t t t c t c a g t a a g a a t c a c c g g c g c t c c c g c a g t g a t g t a g g a c t g g g g a c a c g a g t g t c t a a a g a g t c a t t c t t a g t g g c c g c g a g g g c g t c a c t a

10,680 (in frame with EGFP) FTKVVSPENLHTEEILVCGH (in frame with EGFP) FTKVVSPENLHTEEILVCGH FTKVVSPENLHTEEILVCGH KVLTTDGSFRWVSSIRTQPW t t t t a c t a a a g t g g t a t c t c c t g a a a a c c t c c a t a c a g a a g a a a t c c t c g t c t g c g g c c a a a a a t g a t t t c a c c a t a g a g g a c t t t t g g a g g t a t g t c t t c t t t a g g a g c a g a c g c c g g t

10,740 (in frame with EGFP) SLEVNITTNLDFFLSVAQVQ (in frame with EGFP) SLEVNITTNLDFFLSVAQVQ SLEVNITTNLDFFLSVAQVQ LRSTFIVVLRSKKRDTACTW c a g t c t c g a g g t g a a t a t c a c t a c c a a c c t c g a c t t c t t c c t g t c t g t c g c a c a g g t c c a g t c a g a g c t c c a c t t a t a g t g a t g g t t g g a g c t g a a g a a g g a c a g a c a g c g t g t c c a g g t

10,800 (in frame with EGFP) LLHQLIVANMTGLEPSNKAA (in frame with EGFP) LLHQLIVANMTGLEPSNKAA LLHQLIVANMTGLEPSNKAA SSCWSITALIVPRSGLLLAA g c t g c t g c a c c a g c t g a t t g t g g c c a a t a t g a c a g g c c t g g a a c c c a g c a a c a a g g c t g c c g a c g a c g t g g t c g a c t a a c a c c g g t t a t a c t g t c c g g a c c t t g g g t c g t t g t t c c g a c g

10,860 (in frame with EGFP) EISKQEQKKVDIFDGGMAET (in frame with EGFP) EISKQEQKKVDIFDGGMAET EISKQEQKKVDIFDGGMAET SIELCSCFFTSINSPPMASV c g a a a t t t c c a a g c a g g a a c a g a a g a a g g t c g a t a t a t t t g a c g g a g g c a t g g c c g a g a c g c t t t a a a g g t t c g t c c t t g t c t t c t t c c a g c t a t a t a a a c t g c c t c c g t a c c g g c t c t g

10,920 (in frame with EGFP) SSRYSGAQDSGIGSDSVKIR (in frame with EGFP) SSRYSGAQDSGIGSDSVKIR SSRYSGAQDSGIGSDSVKIR LLRYLPACSLPM a a g c a g c c g g t a c a g t g g c g c a c a g g a c a g c g g c a t t g g a t c t g a t a g t g t c a a g a t c a g t t c g t c g g c c a t g t c a c c g c g t g t c c t g t c g c c g t a a c c t a g a c t a t c a c a g t t c t a g t c

10,980 (in frame with EGFP) IVQIEQHSGASQHRIARPSR (in frame with EGFP) IVQIEQHSGASQHRIARPSR IVQIEQHSGASQHRIARPSR a a t a g t g c a g a t c g a g c a g c a c a g c g g c g c c t c t c a g c a t a g g a t t g c a a g a c c g t c c a g t t a t c a c g t c t a g c t c g t c g t g t c g c c g c g g a g a g t c g t a t c c t a a c g t t c t g g c a g g t c

11,040 (in frame with EGFP) QSSIVKNLNFIPFDIFITAS (in frame with EGFP) QSSIVKNLNFIPFDIFITAS QSSIVKNLNFIPFDIFITAS a c a g t c a a g c a t c g t g a a a a a c c t g a a c t t c a t c c c c t t t g a c a t t t t c a t c a c c g c c t c t g t c a g t t c g t a g c a c t t t t t g g a c t t g a a g t a g g g g a a a c t g t a a a a g t a g t g g c g g a g

11,100 (in frame with EGFP) RISLMTYSCMALSKSKSQEQ (in frame with EGFP) RISLMTYSCMALSKSKSQEQ RISLMTYSCMALSKSKSQEQ a a g a a t c a g c c t g a t g a c c t a t a g c t g c a t g g c t t t g a g c a a g t c a a a g t c c c a a g a a c a t t c t t a g t c g g a c t a c t g g a t a t c g a c g t a c c g a a a c t c g t t c a g t t t c a g g g t t c t t g t

11,160 (in frame with EGFP) KNNEKTDKSSLNLPEVDSDV (in frame with EGFP) KNNEKTDKSSLNLPEVDSDV KNNEKTDKSSLNLPEVDSDV g a a g a a c a a c g a a a a g a c a g a c a a a t c t a g c c t g a a c c t a c c c g a a g t a g a t a g c g a t g t c t t c t t g t t g c t t t t c t g t c t g t t t a g a t c g g a c t t g g a t g g g c t t c a t c t a t c g c t a c a

BbvCI 11,220 (in frame with EGFP) AKPNQACISTVTAEDLLRSS (in frame with EGFP) AKPNQACISTVTAEDLLRSS AKPNQACISTVTAEDLLRSS g g c c a a g c c t a a t c a g g c c t g c a t c a g c a c a g t c a c a g c t g a g g a t c t a c t g a g a t c c t c c c g g t t c g g a t t a g t c c g g a c g t a g t c g t g t c a g t g t c g a c t c c t a g a t g a c t c t a g g a g

11,280 (in frame with EGFP) ISFPSGKKIGVLSLESLHAS (in frame with EGFP) ISFPSGKKIGVLSLESLHAS ISFPSGKKIGVLSLESLHAS c a t c t c t t t t c c t a g t g g t a a a a a a a t c g g g g t c t t a t c a t t g g a a t c g c t g c a c g c c t c g t a g a g a a a a g g a t c a c c a t t t t t t t a g c c c c a g a a t a g t a a c c t t a g c g a c g t g c g g a g

11,340 (in frame with EGFP) TRSSARQALGITIVRQPGRR (in frame with EGFP) TRSSARQALGITIVRQPGRR TRSSARQALGITIVRQPGRR c a c t a g a a g c t c t g c a c g g c a g g c t c t a g g c a t c a c a a t c g t g a g a c a g c c t g g c a g a a g g t g a t c t t c g a g a c g t g c c g t c c g a g a t c c g t a g t g t t a g c a c t c t g t c g g a c c g t c t t c

11,400 (in frame with EGFP) GTGDLQLEPFLYFIVSQPSL (in frame with EGFP) GTGDLQLEPFLYFIVSQPSL GTGDLQLEPFLYFIVSQPSL a g g t a c a g g c g a c c t g c a g c t c g a g c c c t t c c t t t a t t t c a t c g t g t c c c a g c c t a g t c t t c c a t g t c c g c t g g a c g t c g a g c t c g g g a a g g a a a t a a a g t a g c a c a g g g t c g g a t c a g a

11,460 (in frame with EGFP) LLSCHHRKQRVEVSIFDAVL (in frame with EGFP) LLSCHHRKQRVEVSIFDAVL LLSCHHRKQRVEVSIFDAVL c c t g c t g t c g t g c c a t c a c a g a a a g c a g a g a g t g g a g g t t t c c a t c t t c g a c g c c g t t c t g g a c g a c a g c a c g g t a g t g t c t t t c g t c t c t c a c c t c c a a a g g t a g a a g c t g c g g c a a g a

11,520 (in frame with EGFP) KGVASDYKCIDPGKTLPEAL (in frame with EGFP) KGVASDYKCIDPGKTLPEAL KGVASDYKCIDPGKTLPEAL a a a g g g c g t c g c c t c t g a c t a c a a g t g t a t t g a c c c g g g a a a a a c c c t g c c c g a g g c a c t t t t c c c g c a g c g g a g a c t g a t g t t c a c a t a a c t g g g c c c t t t t t g g g a c g g g c t c c g t g a

11,580 (in frame with EGFP) DYCTVWLQTVPGEIDSKSGI (in frame with EGFP) DYCTVWLQTVPGEIDSKSGI DYCTVWLQTVPGEIDSKSGI t g a t t a c t g t a c c g t g t g g c t g c a a a c c g t g c c c g g c g a g a t t g a c t c c a a g t c c g g a a t a c t a a t g a c a t g g c a c a c c g a c g t t t g g c a c g g g c c g c t c t a a c t g a g g t t c a g g c c t t a

11,640 (in frame with EGFP) PPSFITLQIKDFLNGPADVN (in frame with EGFP) PPSFITLQIKDFLNGPADVN PPSFITLQIKDFLNGPADVN c c c t c c c a g t t t c a t t a c a c t g c a a a t c a a a g a t t t c c t c a a c g g c c c t g c c g a c g t g a a g g g a g g g t c a a a g t a a t g t g a c g t t t a g t t t c t a a a g g a g t t g c c g g g a c g g c t g c a c t t

11,700 (in frame with EGFP) LDISKPLKANLSFTKLDQIN (in frame with EGFP) LDISKPLKANLSFTKLDQIN LDISKPLKANLSFTKLDQIN c c t g g a c a t c a g t a a g c c t c t c a a a g c a a a c c t g a g t t t c a c a a a g c t g g a t c a g a t c a a g g a c c t g t a g t c a t t c g g a g a g t t t c g t t t g g a c t c a a a g t g t t t c g a c c t a g t c t a g t t

11,760 (in frame with EGFP) LFLKKIKNAHSLAHSEETSA (in frame with EGFP) LFLKKIKNAHSLAHSEETSA LFLKKIKNAHSLAHSEETSA c c t g t t t c t g a a g a a a a t a a a g a a t g c c c a c t c a c t c g c t c a c t c t g a g g a a a c c t c t g c g g a c a a a g a c t t c t t t t a t t t c t t a c g g g t g a g t g a g c g a g t g a g a c t c c t t t g g a g a c g

11,820 (in frame with EGFP) MSNTMVNKDDLPVSKYYRGK (in frame with EGFP) MSNTMVNKDDLPVSKYYRGK MSNTMVNKDDLPVSKYYRGK c a t g t c t a a t a c c a t g g t g a a c a a a g a c g a t c t g c c c g t g t c a a a g t a c t a t a g a g g c a a g t a c a g a t t a t g g t a c c a c t t g t t t c t g c t a g a c g g g c a c a g t t t c a t g a t a t c t c c g t t

11,880 (in frame with EGFP) LSKPKIHGDGVQKISAQENM (in frame with EGFP) LSKPKIHGDGVQKISAQENM LSKPKIHGDGVQKISAQENM a c t t a g t a a a c c t a a a a t t c a c g g c g a t g g a g t a c a a a a g a t c t c g g c c c a g g a a a a t a t t g a a t c a t t t g g a t t t t a a g t g c c g c t a c c t c a t g t t t t c t a g a g c c g g g t c c t t t t a t a

11,940 (in frame with EGFP) WRAVSCFQKISVQTTQIVIS (in frame with EGFP) WRAVSCFQKISVQTTQIVIS WRAVSCFQKISVQTTQIVIS g t g g c g a g c t g t c t c c t g c t t c c a g a a a a t t t c c g t t c a g a c g a c c c a g a t c g t c a t c a g c a c c g c t c g a c a g a g g a c g a a g g t c t t t t a a a g g c a a g t c t g c t g g g t c t a g c a g t a g t c

12,000 (in frame with EGFP) METVPHTSKPCLLASLSNLN (in frame with EGFP) METVPHTSKPCLLASLSNLN METVPHTSKPCLLASLSNLN t a t g g a a a c c g t t c c t c a c a c c t c g a a g c c t t g t c t g c t c g c t t c t c t g t c c a a t c t g a a a t a c c t t t g g c a a g g a g t g t g g a g c t t c g g a a c a g a c g a g c g a a g a g a c a g g t t a g a c t t

12,060 (in frame with EGFP) GSLSVKATQKVPGIILGSSF (in frame with EGFP) GSLSVKATQKVPGIILGSSF GSLSVKATQKVPGIILGSSF c g g c t c c c t g t c a g t g a a g g c c a c a c a g a a a g t g c c c g g a a t c a t t c t g g g c t c g t c t t t g c c g a g g g a c a g t c a c t t c c g g t g t g t c t t t c a c g g g c c t t a g t a a g a c c c g a g c a g a a a

AflII 12,120 (in frame with EGFP) LLSINDFLLKTSLKERSRIL (in frame with EGFP) LLSINDFLLKTSLKERSRIL LLSINDFLLKTSLKERSRIL c c t g c t g t c a a t c a a c g a c t t c c t g c t g a a g a c c t c c c t t a a g g a a c g g a g c a g a a t c c t g g a c g a c a g t t a g t t g c t g a a g g a c g a c t t c t g g a g g g a a t t c c t t g c c t c g t c t t a g g a

12,180 (in frame with EGFP) IGPCCATANLEAKWCKHSGN (in frame with EGFP) IGPCCATANLEAKWCKHSGN IGPCCATANLEAKWCKHSGN g a t t g g g c c a t g c t g c g c c a c a g c g a a c c t g g a a g c t a a a t g g t g c a a g c a c t c t g g a a a c t a a c c c g g t a c g a c g c g g t g t c g c t t g g a c c t t c g a t t t a c c a c g t t c g t g a g a c c t t t

12,240 (in frame with EGFP) PGPEQSIPKISIDLRGGLLQ (in frame with EGFP) PGPEQSIPKISIDLRGGLLQ PGPEQSIPKISIDLRGGLLQ t c c t g g t c c t g a g c a a t c t a t c c c a a a g a t c a g t a t c g a c c t t a g g g g c g g c c t g c t g c a a g g a c c a g g a c t c g t t a g a t a g g g t t t c t a g t c a t a g c t g g a a t c c c c g c c g g a c g a c g t

12,300 (in frame with EGFP) VFWGQEHLNCLVLLHELLNG (in frame with EGFP) VFWGQEHLNCLVLLHELLNG VFWGQEHLNCLVLLHELLNG a g t g t t c t g g g g a c a g g a g c a c c t c a a t t g c c t g g t g c t g c t g c a c g a g t t a c t g a a t g g t c a c a a g a c c c c t g t c c t c g t g g a g t t a a c g g a c c a c g a c g a c g t g c t c a a t g a c t t a c c

12,360 (in frame with EGFP) YLNEEGNFEVQVSEPVPQMS (in frame with EGFP) YLNEEGNFEVQVSEPVPQMS YLNEEGNFEVQVSEPVPQMS g t a c c t a a a t g a g g a g g g c a a c t t c g a g g t a c a g g t g a g t g a g c c t g t g c c a c a g a t g a g c a t g g a t t t a c t c c t c c c g t t g a a g c t c c a t g t c c a c t c a c t c g g a c a c g g t g t c t a c t c

12,420 (in frame with EGFP) SPVEKNQTFKSEQSSDDLRT (in frame with EGFP) SPVEKNQTFKSEQSSDDLRT SPVEKNQTFKSEQSSDDLRT c a g c c c g g t t g a g a a g a a t c a g a c a t t c a a g a g c g a g c a g a g c t c a g a t g a t c t c a g a a c g t c g g g c c a a c t c t t c t t a g t c t g t a a g t t c t c g c t c g t c t c g a g t c t a c t a g a g t c t t g

12,480 (in frame with EGFP) GLFQYVQDAESLKLPGVYEV (in frame with EGFP) GLFQYVQDAESLKLPGVYEV GLFQYVQDAESLKLPGVYEV t g g c c t g t t c c a g t a c g t t c a g g a t g c c g a g a g c c t g a a g c t g c c t g g a g t g t a c g a a g t a c c g g a c a a g g t c a t g c a a g t c c t a c g g c t c t c g g a c t t c g a c g g a c c t c a c a t g c t t c a

12,540 (in frame with EGFP) LFYNETEDCPGMMLWRYPEP (in frame with EGFP) LFYNETEDCPGMMLWRYPEP LFYNETEDCPGMMLWRYPEP t c t a t t c t a c a a c g a g a c t g a a g a c t g c c c c g g c a t g a t g c t c t g g c g g t a c c c a g a a c c a g a t a a g a t g t t g c t c t g a c t t c t g a c g g g g c c g t a c t a c g a g a c c g c c a t g g g t c t t g g

FspAI 12,600 (in frame with EGFP) RVLTLVRITPVPFNTTEDPD (in frame with EGFP) RVLTLVRITPVPFNTTEDPD RVLTLVRITPVPFNTTEDPD t a g a g t g c t g a c c c t g g t g c g c a t c a c a c c a g t g c c a t t c a a c a c c a c c g a a g a t c c t g a a t c t c a c g a c t g g g a c c a c g c g t a g t g t g g t c a c g g t a a g t t g t g g t g g c t t c t a g g a c t

12,660 (in frame with EGFP) ISTADLGDVLQVPCSLEYWD (in frame with EGFP) ISTADLGDVLQVPCSLEYWD ISTADLGDVLQVPCSLEYWD t a t c a g t a c c g c c g a c c t g g g g g a t g t g c t g c a g g t c c c t t g c a g c c t c g a a t a c t g g g a a t a g t c a t g g c g g c t g g a c c c c c t a c a c g a c g t c c a g g g a a c g t c g g a g c t t a t g a c c c t

12,720 (in frame with EGFP) ELQKVFVAFREFNLSESKVC (in frame with EGFP) ELQKVFVAFREFNLSESKVC ELQKVFVAFREFNLSESKVC t g a g c t g c a g a a g g t a t t c g t g g c c t t t a g a g a g t t c a a t c t g t c t g a a t c a a a g g t g t g a c t c g a c g t c t t c c a t a a g c a c c g g a a a t c t c t c a a g t t a g a c a g a c t t a g t t t c c a c a c

12,780 (in frame with EGFP) ELQLPDINLVNDQKKLVSSD (in frame with EGFP) ELQLPDINLVNDQKKLVSSD ELQLPDINLVNDQKKLVSSD c g a g c t g c a a t t a c c t g a c a t c a a c c t c g t t a a c g a c c a g a a g a a a c t g g t t t c t a g c g a g c t c g a c g t t a a t g g a c t g t a g t t g g a g c a a t t g c t g g t c t t c t t t g a c c a a a g a t c g c t

12,840 (in frame with EGFP) LWRIVLNSSQNGADDQSSAS (in frame with EGFP) LWRIVLNSSQNGADDQSSAS LWRIVLNSSQNGADDQSSAS c c t g t g g c g g a t c g t t c t t a a c a g c t c a c a a a a c g g c g c c g a t g a t c a g t c c a g t g c c t c g g a c a c c g c c t a g c a a g a a t t g t c g a g t g t t t t g c c g c g g c t a c t a g t c a g g t c a c g g a g

12,900 (in frame with EGFP) ESGSQSTCDPLVTPTALAAC (in frame with EGFP) ESGSQSTCDPLVTPTALAAC ESGSQSTCDPLVTPTALAAC g g a a t c t g g c t c c c a g t c t a c c t g c g a c c c t c t c g t c a c t c c g a c c g c g c t c g c c g c c t g c c t t a g a c c g a g g g t c a g a t g g a c g c t g g g a g a g c a g t g a g g c t g g c g c g a g c g g c g g a c

12,960 (in frame with EGFP) TRVDSCFTPWFVPSLCVSFQ (in frame with EGFP) TRVDSCFTPWFVPSLCVSFQ TRVDSCFTPWFVPSLCVSFQ c a c a c g g g t g g a t a g c t g c t t t a c c c c a t g g t t c g t g c c c t c t c t g t g c g t g a g c t t c c a g t g t g c c c a c c t a t c g a c g a a a t g g g g t a c c a a g c a c g g g a g a g a c a c g c a c t c g a a g g t

13,020 (in frame with EGFP) FAHLEFHLCHHLDQLGTAAP (in frame with EGFP) FAHLEFHLCHHLDQLGTAAP FAHLEFHLCHHLDQLGTAAP g t t c g c c c a c t t g g a a t t t c a c t t a t g c c a c c a t c t g g a t c a a c t g g g c a c c g c c g c c c c c a a g c g g g t g a a c c t t a a a g t g a a t a c g g t g g t a g a c c t a g t t g a c c c g t g g c g g c g g g g

13,080 (in frame with EGFP) QYLQPFVSDRNMPSELEYMI (in frame with EGFP) QYLQPFVSDRNMPSELEYMI QYLQPFVSDRNMPSELEYMI c c a a t a c c t t c a g c c c t t c g t g a g c g a c c g g a a c a t g c c t a g c g a g t t g g a g t a c a t g a t g g t t a t g g a a g t c g g g a a g c a c t c g c t g g c c t t g t a c g g a t c g c t c a a c c t c a t g t a c t a

13,140 (in frame with EGFP) VSFREPHMYLRQWNNGSVCQ (in frame with EGFP) VSFREPHMYLRQWNNGSVCQ VSFREPHMYLRQWNNGSVCQ t g t g t c t t t c a g a g a g c c c c a t a t g t a c c t g a g a c a g t g g a a c a a c g g g t c t g t a t g c c a a c a c a g a a a g t c t c t c g g g g t a t a c a t g g a c t c t g t c a c c t t g t t g c c c a g a c a t a c g g t

13,200 (in frame with EGFP) EIQFLAQADCKLLECRNVTM (in frame with EGFP) EIQFLAQADCKLLECRNVTM EIQFLAQADCKLLECRNVTM g g a g a t c c a a t t c c t g g c c c a g g c g g a c t g c a a g c t c c t g g a g t g c a g a a a c g t g a c a a t c c t c t a g g t t a a g g a c c g g g t c c g c c t g a c g t t c g a g g a c c t c a c g t c t t t g c a c t g t t a

13,260 (in frame with EGFP) QSVVKPFSIFGQMAVSSDVV (in frame with EGFP) QSVVKPFSIFGQMAVSSDVV QSVVKPFSIFGQMAVSSDVV g c a g a g t g t g g t g a a a c c t t t t t c t a t c t t c g g t c a g a t g g c c g t g t c c t c c g a c g t g g t c g t c t c a c a c c a c t t t g g a a a a a g a t a g a a g c c a g t c t a c c g g c a c a g g a g g c t g c a c c a

13,320 (in frame with EGFP) EKLLDCTVIVDSVFVNLGQH (in frame with EGFP) EKLLDCTVIVDSVFVNLGQH EKLLDCTVIVDSVFVNLGQH g g a g a a a c t g t t a g a t t g t a c c g t t a t c g t a g a c a g c g t c t t t g t a a a c c t g g g c c a g c a c c t c t t t g a c a a t c t a a c a t g g c a a t a g c a t c t g t c g c a g a a a c a t t t g g a c c c g g t c g t

13,380 (in frame with EGFP) VVHSLNTAIQAWQQNKCPEV (in frame with EGFP) VVHSLNTAIQAWQQNKCPEV VVHSLNTAIQAWQQNKCPEV t g t g g t t c a t t c g t t g a a c a c t g c g a t c c a g g c t t g g c a g c a a a a t a a g t g c c c t g a a g t a c a c c a a g t a a g c a a c t t g t g a c g c t a g g t c c g a a c c g t c g t t t t a t t c a c g g g a c t t c a

13,440 (in frame with EGFP) EELVFSHFVICNDTQETLRF (in frame with EGFP) EELVFSHFVICNDTQETLRF EELVFSHFVICNDTQETLRF g g a g g a a c t g g t g t t c t c g c a t t t c g t g a t c t g t a a c g a c a c a c a g g a g a c c c t g a g a t t c c t c c t t g a c c a c a a g a g c g t a a a g c a c t a g a c a t t g c t g t g t g t c c t c t g g g a c t c t a a

13,500 (in frame with EGFP) GQVDTDENILLASLHSHQYS (in frame with EGFP) GQVDTDENILLASLHSHQYS GQVDTDENILLASLHSHQYS c g g c c a g g t a g a c a c c g a c g a a a a t a t t t t g c t t g c c t c t c t a c a c a g t c a c c a a t a c t c g c c g g t c c a t c t g t g g c t g c t t t t a t a a a a c g a a c g g a g a g a t g t g t c a g t g g t t a t g a g

13,560 (in frame with EGFP) WRSHKSPQLLHICIEGWGNW (in frame with EGFP) WRSHKSPQLLHICIEGWGNW WRSHKSPQLLHICIEGWGNW c t g g a g a t c c c a c a a g t c c c c t c a a c t c c t g c a c a t c t g c a t c g a g g g a t g g g g c a a c t g g a c c t c t a g g g t g t t c a g g g g a g t t g a g g a c g t g t a g a c g t a g c t c c c t a c c c c g t t g a c

13,620 (in frame with EGFP) RWSEPFSVDHAGTFIRTIQY (in frame with EGFP) RWSEPFSVDHAGTFIRTIQY RWSEPFSVDHAGTFIRTIQY g c g g t g g a g c g a g c c a t t c t c c g t g g a c c a c g c c g g c a c t t t t a t t c g g a c c a t a c a a t a c g c c a c c t c g c t c g g t a a g a g g c a c c t g g t g c g g c c g t g a a a a t a a g c c t g g t a t g t t a t

BmtINheI 13,680 (in frame with EGFP) RGRTASLIIKVQQLNGVQKQ (in frame with EGFP) RGRTASLIIKVQQLNGVQKQ RGRTASLIIKVQQLNGVQKQ t c g g g g g a g a a c c g c t a g c c t c a t c a t c a a g g t t c a g c a g c t g a a c g g g g t c c a g a a g c a a g c c c c c t c t t g g c g a t c g g a g t a g t a g t t c c a a g t c g t c g a c t t g c c c c a g g t c t t c g t

13,740 (in frame with EGFP) IIICGRQIICSYLSQSIELK (in frame with EGFP) IIICGRQIICSYLSQSIELK IIICGRQIICSYLSQSIELK a a t c a t t a t c t g t g g c c g t c a a a t c a t c t g c t c a t a c c t g t c t c a g t c c a t c g a g c t g a a t t a g t a a t a g a c a c c g g c a g t t t a g t a g a c g a g t a t g g a c a g a g t c a g g t a g c t c g a c t t

13,800 (in frame with EGFP) VVQHYIGQDGQAVVREHFDC (in frame with EGFP) VVQHYIGQDGQAVVREHFDC VVQHYIGQDGQAVVREHFDC g g t g g t g c a g c a c t a c a t t g g c c a a g a t g g c c a g g c c g t a g t t c g g g a a c a c t t c g a t t g c c a c c a c g t c g t g a t g t a a c c g g t t c t a c c g g t c c g g c a t c a a g c c c t t g t g a a g c t a a c

13,860 (in frame with EGFP) LTAKQKLPSYILENNELTEL (in frame with EGFP) LTAKQKLPSYILENNELTEL LTAKQKLPSYILENNELTEL c c t g a c c g c c a a g c a g a a g c t g c c t t c c t a c a t c c t g g a g a a c a a t g a g c t t a c a g a g c t g g a c t g g c g g t t c g t c t t c g a c g g a a g g a t g t a g g a c c t c t t g t t a c t c g a a t g t c t c g a

13,920 (in frame with EGFP) CVKAKGDEDWSRDVCLESKA (in frame with EGFP) CVKAKGDEDWSRDVCLESKA CVKAKGDEDWSRDVCLESKA g t g c g t g a a g g c g a a g g g g g a c g a g g a c t g g t c c a g a g a t g t t t g t t t a g a a a g c a a a g c c a c g c a c t t c c g c t t c c c c c t g c t c c t g a c c a g g t c t c t a c a a a c a a a t c t t t c g t t t c g

13,980 (in frame with EGFP) PEYSIVIQVPSSNSSIIYVW (in frame with EGFP) PEYSIVIQVPSSNSSIIYVW PEYSIVIQVPSSNSSIIYVW a c c g g a a t a c t c t a t c g t a a t c c a g g t g c c a t c t a g c a a c t c g t c t a t c a t c t a c g t g t g t g g c c t t a t g a g a t a g c a t t a g g t c c a c g g t a g a t c g t t g a g c a g a t a g t a g a t g c a c a c

14,040 (in frame with EGFP) CTVLTLEPNSQVQQRMIVFS (in frame with EGFP) CTVLTLEPNSQVQQRMIVFS CTVLTLEPNSQVQQRMIVFS g t g c a c c g t g c t g a c a c t g g a g c c c a a c a g c c a a g t g c a g c a a a g a a t g a t c g t g t t t t c c a c g t g g c a c g a c t g t g a c c t c g g g t t g t c g g t t c a c g t c g t t t c t t a c t a g c a c a a a a g

14,100 (in frame with EGFP) PLFIMRSHLPDPIIIHLEKR (in frame with EGFP) PLFIMRSHLPDPIIIHLEKR PLFIMRSHLPDPIIIHLEKR c c c a c t g t t c a t c a t g a g a t c c c a c c t g c c a g a c c c a a t c a t c a t c c a c c t a g a a a a g a g g g g t g a c a a g t a g t a c t c t a g g g t g g a c g g t c t g g g t t a g t a g t a g g t g g a t c t t t t c t c

14,160 (in frame with EGFP) SLGLSETQIIPGKGQEKPLQ (in frame with EGFP) SLGLSETQIIPGKGQEKPLQ SLGLSETQIIPGKGQEKPLQ a t c t c t c g g c c t g t c c g a g a c t c a g a t c a t a c c t g g t a a g g g c c a a g a a a a a c c a c t g c a t a g a g a g c c g g a c a g g c t c t g a g t c t a g t a t g g a c c a t t c c c g g t t c t t t t t g g t g a c g t

14,220 (in frame with EGFP) NIEPDLVHHLTFQAREEYDP (in frame with EGFP) NIEPDLVHHLTFQAREEYDP NIEPDLVHHLTFQAREEYDP g a a t a t c g a g c c t g a t c t g g t g c a c c a c c t g a c c t t c c a g g c t a g a g a a g a g t a c g a t c c c t t a t a g c t c g g a c t a g a c c a c g t g g t g g a c t g g a a g g t c c g a t c t c t t c t c a t g c t a g g

14,280 (in frame with EGFP) SDCAVPISTSLIKQIATKVH (in frame with EGFP) SDCAVPISTSLIKQIATKVH SDCAVPISTSLIKQIATKVH c t c c g a c t g c g c c g t c c c a a t c t c t a c c t c t c t t a t c a a g c a g a t c g c a a c a a a a g t g c a g a g g c t g a c g c g g c a g g g t t a g a g a t g g a g a g a a t a g t t c g t c t a g c g t t g t t t t c a c g t

14,340 (in frame with EGFP) PGGTVNQILDEFYGPEKSLQ (in frame with EGFP) PGGTVNQILDEFYGPEKSLQ PGGTVNQILDEFYGPEKSLQ c c c c g g c g g c a c c g t c a a c c a g a t c c t g g a c g a g t t c t a c g g c c c t g a a a a g a g c c t g c a g g g g c c g c c g t g g c a g t t g g t c t a g g a c c t g c t c a a g a t g c c g g g a c t t t t c t c g g a c g t

14,400 (in frame with EGFP) PIWPYNKKDSDRNEQLSQWD (in frame with EGFP) PIWPYNKKDSDRNEQLSQWD PIWPYNKKDSDRNEQLSQWD g c c c a t c t g g c c t t a c a a c a a a a a g g a t t c c g a c a g a a a c g a g c a g c t g t c a c a g t g g g a c g g g t a g a c c g g a a t g t t g t t t t t c c t a a g g c t g t c t t t g c t c g t c g a c a g t g t c a c c c t

14,460 (in frame with EGFP) SPMRVKLSIWKPYVRTLLIE (in frame with EGFP) SPMRVKLSIWKPYVRTLLIE SPMRVKLSIWKPYVRTLLIE c a g t c c a a t g c g g g t g a a g t t a t c a a t c t g g a a a c c c t a c g t g c g g a c g t t a c t g a t a g a g t c a g g t t a c g c c c a c t t c a a t a g t t a g a c c t t t g g g a t g c a c g c c t g c a a t g a c t a t c t

14,520 (in frame with EGFP) LLPWALLINESKWDLWLFEG (in frame with EGFP) LLPWALLINESKWDLWLFEG LLPWALLINESKWDLWLFEG g c t g t t a c c t t g g g c g t t g c t g a t a a a c g a g t c a a a g t g g g a c c t g t g g c t g t t t g a g g g c g a c a a t g g a a c c c g c a a c g a c t a t t t g c t c a g t t t c a c c c t g g a c a c c g a c a a a c t c c c

14,580 (in frame with EGFP) EKIVLQVPAGKIIIPPNFQE (in frame with EGFP) EKIVLQVPAGKIIIPPNFQE EKIVLQVPAGKIIIPPNFQE c g a g a a a a t c g t g c t t c a a g t a c c a g c c g g c a a g a t c a t t a t c c c t c c a a a c t t c c a g g a g c t c t t t t a g c a c g a a g t t c a t g g t c g g c c g t t c t a g t a a t a g g g a g g t t t g a a g g t c c t

14,640 (in frame with EGFP) AFQIGIYWANTNTVHKSVAI (in frame with EGFP) AFQIGIYWANTNTVHKSVAI AFQIGIYWANTNTVHKSVAI g g c c t t t c a g a t c g g c a t c t a c t g g g c c a a c a c c a a c a c c g t c c a t a a g t c t g t g g c c a t c c g g a a a g t c t a g c c g t a g a t g a c c c g g t t g t g g t t g t g g c a g g t a t t c a g a c a c c g g t a

14,700 (in frame with EGFP) KLVHNLTSPKWKDGGNGEVV (in frame with EGFP) KLVHNLTSPKWKDGGNGEVV KLVHNLTSPKWKDGGNGEVV t a a g t t a g t g c a t a a c t t g a c c t c t c c t a a a t g g a a g g a c g g c g g c a a c g g a g a g g t t g t a t t c a a t c a c g t a t t g a a c t g g a g a g g a t t t a c c t t c c t g c c g c c g t t g c c t c t c c a a c a

14,760 (in frame with EGFP) TLDEEAFVDTEIRLGAFPGH (in frame with EGFP) TLDEEAFVDTEIRLGAFPGH TLDEEAFVDTEIRLGAFPGH g a c c c t g g a c g a a g a g g c c t t c g t g g a c a c c g a g a t c a g a c t t g g t g c a t t c c c t g g c c a c t g g g a c c t g c t t c t c c g g a a g c a c c t g t g g c t c t a g t c t g a a c c a c g t a a g g g a c c g g t

14,820 (in frame with EGFP) QKLCQFCISSMVQQGIQIIQ (in frame with EGFP) QKLCQFCISSMVQQGIQIIQ QKLCQFCISSMVQQGIQIIQ c c a g a a g c t g t g t c a g t t c t g c a t c a g c t c c a t g g t c c a g c a g g g c a t c c a g a t c a t t c a g g t c t t c g a c a c a g t c a a g a c g t a g t c g a g g t a c c a g g t c g t c c c g t a g g t c t a g t a a g t

14,880 (in frame with EGFP) IEDKTTIINNTPYQIFYKPQ (in frame with EGFP) IEDKTTIINNTPYQIFYKPQ IEDKTTIINNTPYQIFYKPQ g a t t g a a g a c a a g a c a a c c a t t a t c a a c a a c a c a c c c t a c c a g a t c t t c t a c a a a c c t c a c t a a c t t c t g t t c t g t t g g t a a t a g t t g t t g t g t g g g a t g g t c t a g a a g a t g t t t g g a g t

14,940 (in frame with EGFP) LSVCNPHSGKEYFRVPDSAT (in frame with EGFP) LSVCNPHSGKEYFRVPDSAT LSVCNPHSGKEYFRVPDSAT g t t a t c c g t g t g t a a c c c t c a c a g c g g c a a g g a a t a t t t c c g a g t g c c t g a t t c t g c c a c c a a t a g g c a c a c a t t g g g a g t g t c g c c g t t c c t t a t a a a g g c t c a c g g a c t a a g a c g g t g

15,000 (in frame with EGFP) FSICPGGEQPAMKSSSLPCW (in frame with EGFP) FSICPGGEQPAMKSSSLPCW FSICPGGEQPAMKSSSLPCW g t t c t c a a t c t g c c c t g g c g g a g a g c a g c c t g c t a t g a a g t c c t c g t c c c t g c c t t g c t g c a a g a g t t a g a c g g g a c c g c c t c t c g t c g g a c g a t a c t t c a g g a g c a g g g a c g g a a c g a c

PshAI 15,060 (in frame with EGFP) DLMPDISQSVLDASLLQKQI (in frame with EGFP) DLMPDISQSVLDASLLQKQI DLMPDISQSVLDASLLQKQI g g a t c t g a t g c c a g a c a t c t c c c a g a g c g t a c t c g a c g c c a g t c t c c t a c a g a a a c a g a t c c t a g a c t a c g g t c t g t a g a g g g t c t c g c a t g a g c t g c g g t c a g a g g a t g t c t t t g t c t a

15,120 (in frame with EGFP) MLGFSPAPGADSSQCWSLPA (in frame with EGFP) MLGFSPAPGADSSQCWSLPA MLGFSPAPGADSSQCWSLPA c a t g c t g g g t t t c a g t c c a g c t c c a g g c g c c g a c a g c a g c c a a t g t t g g a g c c t t c c t g c g t a c g a c c c a a a g t c a g g t c g a g g t c c g c g g c t g t c g t c g g t t a c a a c c t c g g a a g g a c g

15,180 (in frame with EGFP) IVRPEFPRQSVAVPLGNFRE (in frame with EGFP) IVRPEFPRQSVAVPLGNFRE IVRPEFPRQSVAVPLGNFRE t a t a g t t c g g c c c g a a t t c c c t c g g c a g a g c g t g g c c g t g c c t t t a g g a a a t t t c a g a g a a t a t c a a g c c g g g c t t a a g g g a g c c g t c t c g c a c c g g c a c g g a a a t c c t t t a a a g t c t c t

15,240 (in frame with EGFP) NGFCTRAIVLTYQEHLGVTY (in frame with EGFP) NGFCTRAIVLTYQEHLGVTY NGFCTRAIVLTYQEHLGVTY a a a c g g c t t t t g c a c a a g a g c c a t t g t g c t a a c a t a c c a a g a g c a c c t g g g a g t g a c t t a t t t g c c g a a a a c g t g t t c t c g g t a a c a c g a t t g t a t g g t t c t c g t g g a c c c t c a c t g a a t

15,300 (in frame with EGFP) LTLSEDPSPRVIIHNRCPVK (in frame with EGFP) LTLSEDPSPRVIIHNRCPVK LTLSEDPSPRVIIHNRCPVK c c t g a c g c t g a g c g a a g a t c c a t c t c c c a g a g t c a t c a t c c a c a a c a g a t g c c c c g t t a a g g a c t g c g a c t c g c t t c t a g g t a g a g g g t c t c a g t a g t a g g t g t t g t c t a c g g g g c a a t t

15,360 (in frame with EGFP) MLIKENIKDIPKFEVYCKKI (in frame with EGFP) MLIKENIKDIPKFEVYCKKI MLIKENIKDIPKFEVYCKKI g a t g c t g a t t a a g g a g a a c a t t a a g g a c a t c c c g a a g t t c g a a g t g t a c t g c a a g a a a a t c t a c g a c t a a t t c c t c t t g t a a t t c c t g t a g g g c t t c a a g c t t c a c a t g a c g t t c t t t t a

15,420 (in frame with EGFP) PSECSIHHELYHQISSYPDC (in frame with EGFP) PSECSIHHELYHQISSYPDC PSECSIHHELYHQISSYPDC c c c g t c c g a g t g c a g c a t a c a c c a t g a a c t c t a c c a c c a g a t t t c c a g c t a c c c c g a t t g g g g c a g g c t c a c g t c g t a t g t g g t a c t t g a g a t g g t g g t c t a a a g g t c g a t g g g g c t a a c

15,480 (in frame with EGFP) KTKDLLPSLLLRVEPLDEVT (in frame with EGFP) KTKDLLPSLLLRVEPLDEVT KTKDLLPSLLLRVEPLDEVT c a a g a c g a a g g a c c t g c t g c c c a g c t t g c t g c t g a g a g t g g a a c c t c t g g a c g a g g t g a c g t t c t g c t t c c t g g a c g a c g g g t c g a a c g a c g a c t c t c a c c t t g g a g a c c t g c t c c a c t g

15,540 (in frame with EGFP) TEWSDAIDINSQGTQVVFLT (in frame with EGFP) TEWSDAIDINSQGTQVVFLT TEWSDAIDINSQGTQVVFLT t a c a g a g t g g t c t g a c g c c a t c g a c a t c a a c a g c c a g g g c a c a c a a g t a g t t t t c t t g a c a t g t c t c a c c a g a c t g c g g t a g c t g t a g t t g t c g g t c c c g t g t g t t c a t c a a a a g a a c t g

15,600 (in frame with EGFP) GFGYVYVDVVHQCGTVFITV (in frame with EGFP) GFGYVYVDVVHQCGTVFITV GFGYVYVDVVHQCGTVFITV *WHPVTNMVT a g g a t t t g g c t a c g t c t a c g t c g a t g t c g t t c a c c a g t g t g g c a c t g t g t t c a t t a c t g t t c c t a a a c c g a t g c a g a t g c a g c t a c a g c a a g t g g t c a c a c c g t g a c a c a a g t a a t g a c a

15,660 (in frame with EGFP) APEGKAGPILTNTNRAPEKI (in frame with EGFP) APEGKAGPILTNTNRAPEKI APEGKAGPILTNTNRAPEKI AGSPFAPGMRVLVLLAGSFI a g c c c c t g a a g g g a a g g c t g g c c c c a t t c t c a c c a a c a c c a a c a g a g c c c c g g a a a a a a t t c g g g g a c t t c c c t t c c g a c c g g g g t a a g a g t g g t t g t g g t t g t c t c g g g g c c t t t t t t a

15,720 (in frame with EGFP) VTFKMFITQLSLAVFDDLTH (in frame with EGFP) VTFKMFITQLSLAVFDDLTH VTFKMFITQLSLAVFDDLTH TVKLINMVCSDNATNSSRVW t g t g a c c t t t a a g a t g t t c a t c a c a c a a c t g t c a t t g g c t g t g t t t g a t g a t c t g a c c c a a c a c t g g a a a t t c t a c a a g t a g t g t g t t g a c a g t a a c c g a c a c a a a c t a c t a g a c t g g g t

15,780 (in frame with EGFP) HKASAELLRLTLDNIFLCVA (in frame with EGFP) HKASAELLRLTLDNIFLCVA HKASAELLRLTLDNIFLCVA WLALASSRRSVKSLMKRHTA c c a c a a a g c c a g c g c g g a g c t c c t g c g a c t c a c c t t a g a t a a c a t c t t t c t g t g c g t c g c g g t g t t t c g g t c g c g c c t c g a g g a c g c t g a g t g g a a t c t a t t g t a g a a a g a c a c g c a g c g

15,840 (in frame with EGFP) PGAGPLPGEEPVAALFELYC (in frame with EGFP) PGAGPLPGEEPVAALFELYC PGAGPLPGEEPVAALFELYC GPAPGSGPSSGTAARNSSYQ c c c a g g c g c t g g c c c a c t g c c t g g c g a g g a a c c c g t c g c c g c c c t g t t c g a g c t g t a t t g g g g t c c g c g a c c g g g t g a c g g a c c g c t c c t t g g g c a g c g g c g g g a c a a g c t c g a c a t a a c

15,900 (in frame with EGFP) VEICCGDLQLDNQLYNKSNF (in frame with EGFP) VEICCGDLQLDNQLYNKSNF VEICCGDLQLDNQLYNKSNF TSIQQPSRCSSLWSYLLLLK c g t t g a g a t t t g c t g c g g c g a c c t a c a a c t g g a c a a c c a g c t g t a c a a c a a a a g c a a t t t g c a a c t c t a a a c g a c g c c g c t g g a t g t t g a c c t g t t g g t c g a c a t g t t g t t t t c g t t a a a

15,960 (in frame with EGFP) HFAVLVCQGEKAEPIQCSKM (in frame with EGFP) HFAVLVCQGEKAEPIQCSKM HFAVLVCQGEKAEPIQCSKM WKATRTQWPSFASGIWHLLI c c a c t t t g c c g t c c t g g t t t g c c a g g g c g a a a a g g c t g a a c c a a t c c a g t g t a g c a a g a t g g t g a a a c g g c a g g a c c a a a c g g t c c c g c t t t t c c g a c t t g g t t a g g t c a c a t c g t t c t a

16,020 (in frame with EGFP) QSLLISNKELEEYKEKCFIK (in frame with EGFP) QSLLISNKELEEYKEKCFIK QSLLISNKELEEYKEKCFIK CLRSILLLSNSSYLSFHKML g c a g a g c c t g c t t a t c a g c a a c a a g g a a t t g g a g g a g t a c a a g g a a a a g t g t t t c a t c a a c g t c t c g g a c g a a t a g t c g t t g t t c c t t a a c c t c c t c a t g t t c c t t t t c a c a a a g t a g t t

16,080 (in frame with EGFP) LCITLNEGKSILCDINEFSF (in frame with EGFP) LCITLNEGKSILCDINEFSF LCITLNEGKSILCDINEFSF SHMVRFSPLDM g c t g t g c a t t a c c c t g a a c g a a g g c a a a t c c a t c c t c t g c g a t a t c a a t g a a t t t a g c t t c g a c a c g t a a t g g g a c t t g c t t c c g t t t a g g t a g g a g a c g c t a t a g t t a c t t a a a t c g a a

16,140 (in frame with EGFP) ELKPARLYVEDTFVYYIKTL (in frame with EGFP) ELKPARLYVEDTFVYYIKTL ELKPARLYVEDTFVYYIKTL c g a a c t c a a a c c a g c c a g a c t g t a c g t t g a g g a t a c a t t c g t g t a c t a c a t c a a g a c t c t g c t t g a g t t t g g t c g g t c t g a c a t g c a a c t c c t a t g t a a g c a c a t g a t g t a g t t c t g a g a

16,200 (in frame with EGFP) FDTYLPNSRLAGHSTHLSGG (in frame with EGFP) FDTYLPNSRLAGHSTHLSGG FDTYLPNSRLAGHSTHLSGG c t t c g a c a c a t a c c t g c c t a a c a g c c g t c t a g c c g g a c a c a g t a c c c a c c t g a g c g g t g g g a a g c t g t g t a t g g a c g g a t t g t c g g c a g a t c g g c c t g t g t c a t g g g t g g a c t c g c c a c c

SrfIBstEII 16,260 (in frame with EGFP) KQVLPMQVTQHARALVNPVK (in frame with EGFP) KQVLPMQVTQHARALVNPVK KQVLPMQVTQHARALVNPVK c a a g c a g g t g c t g c c t a t g c a g g t g a c c c a g c a t g c c c g g g c c c t g g t c a a c c c c g t a a a g t t c g t c c a c g a c g g a t a c g t c c a c t g g g t c g t a c g g g c c c g g g a c c a g t t g g g g c a t t t

16,320 (in frame with EGFP) LRKLVIQPVNLLVSIHASLK (in frame with EGFP) LRKLVIQPVNLLVSIHASLK LRKLVIQPVNLLVSIHASLK g c t g a g a a a a c t g g t t a t c c a g c c a g t a a a c c t g c t g g t g a g c a t c c a t g c g t c g c t g a a c g a c t c t t t t g a c c a a t a g g t c g g t c a t t t g g a c g a c c a c t c g t a g g t a c g c a g c g a c t t

16,380 (in frame with EGFP) LYIASDHTPLSFSVFERGPI (in frame with EGFP) LYIASDHTPLSFSVFERGPI LYIASDHTPLSFSVFERGPI g t t a t a t a t c g c c a g c g a c c a t a c c c c g c t g a g c t t c t c t g t g t t t g a g c g a g g a c c c a t c a a t a t a t a g c g g t c g c t g g t a t g g g g c g a c t c g a a g a g a c a c a a a c t c g c t c c t g g g t a

MreI 16,440 (in frame with EGFP) FTTARQLVHALAMHYAAGAL (in frame with EGFP) FTTARQLVHALAMHYAAGAL FTTARQLVHALAMHYAAGAL c t t t a c c a c a g c c a g a c a g c t g g t g c a c g c c c t g g c a a t g c a c t a c g c c g c c g g c g c c c t g a a a t g g t g t c g g t c t g t c g a c c a c g t g c g g g a c c g t t a c g t g a t g c g g c g g c c g c g g g a

16,500 (in frame with EGFP) FRAGWVVGSLDILGSPASLV (in frame with EGFP) FRAGWVVGSLDILGSPASLV FRAGWVVGSLDILGSPASLV c t t t c g g g c t g g c t g g g t g g t a g g c a g c t t g g a t a t c c t c g g t a g c c c t g c c a g c c t g g t g a a a g c c c g a c c g a c c c a c c a t c c g t c g a a c c t a t a g g a g c c a t c g g g a c g g t c g g a c c a

16,560 (in frame with EGFP) RSIGNGVADFFRLPYEGLTR (in frame with EGFP) RSIGNGVADFFRLPYEGLTR RSIGNGVADFFRLPYEGLTR g c g a t c c a t c g g a a a c g g c g t g g c t g a c t t c t t c c g a c t g c c c t a c g a a g g c c t g a c c a g c g c t a g g t a g c c t t t g c c g c a c c g a c t g a a g a a g g c t g a c g g g a t g c t t c c g g a c t g g t c

16,620 (in frame with EGFP) GPGAFVSGVSRGTTSFVKHI (in frame with EGFP) GPGAFVSGVSRGTTSFVKHI GPGAFVSGVSRGTTSFVKHI a g g c c c t g g c g c c t t c g t t a g t g g c g t t a g c a g a g g c a c t a c c t c a t t c g t g a a g c a c a t t c c g g g a c c g c g g a a g c a a t c a c c g c a a t c g t c t c c g t g a t g g a g t a a g c a c t t c g t g t a

16,680 (in frame with EGFP) SKGTLTSITNLATSLARNMD (in frame with EGFP) SKGTLTSITNLATSLARNMD SKGTLTSITNLATSLARNMD c t c t a a g g g a a c c c t t a c t t c a a t c a c c a a c t t a g c c a c a t c t c t c g c a a g a a a c a t g g a g a g a t t c c c t t g g g a a t g a a g t t a g t g g t t g a a t c g g t g t a g a g a g c g t t c t t t g t a c c t

16,740 (in frame with EGFP) RLSLDEEHYNRQEEWRRQLP (in frame with EGFP) RLSLDEEHYNRQEEWRRQLP RLSLDEEHYNRQEEWRRQLP c a g a c t t a g c c t c g a t g a g g a a c a c t a c a a c c g g c a g g a g g a g t g g c g g a g a c a g c t g c c g t c t g a a t c g g a g c t a c t c c t t g t g a t g t t g g c c g t c c t c c t c a c c g c c t c t g t c g a c g g

16,800 (in frame with EGFP) ESLGEGLRQGLSRLGISLLG (in frame with EGFP) ESLGEGLRQGLSRLGISLLG ESLGEGLRQGLSRLGISLLG t g a g t c g c t g g g g g a g g g a c t g a g g c a g g g c c t g t c t c g g t t a g g c a t c a g c c t g t t a g g a c t c a g c g a c c c c c t c c c t g a c t c c g t c c c g g a c a g a g c c a a t c c g t a g t c g g a c a a t c c

16,860 (in frame with EGFP) AIAGIVDQPMQNFQKTSEAQ (in frame with EGFP) AIAGIVDQPMQNFQKTSEAQ AIAGIVDQPMQNFQKTSEAQ a g c c a t c g c g g g a a t c g t g g a c c a g c c t a t g c a g a a t t t t c a g a a g a c t t c t g a a g c c c a t c g g t a g c g c c c t t a g c a c c t g g t c g g a t a c g t c t t a a a a g t c t t c t g a a g a c t t c g g g t

16,920 (in frame with EGFP) ASAGHKAKGVISGVGKGIMG (in frame with EGFP) ASAGHKAKGVISGVGKGIMG ASAGHKAKGVISGVGKGIMG g g c c a g t g c c g g c c a c a a g g c t a a g g g a g t c a t c a g c g g c g t t g g c a a g g g g a t c a t g g g c c g g t c a c g g c c g g t g t t c c g a t t c c c t c a g t a g t c g c c g c a a c c g t t c c c c t a g t a c c c

16,980 (in frame with EGFP) VFTKPIGGAAELVSQTGYGI (in frame with EGFP) VFTKPIGGAAELVSQTGYGI VFTKPIGGAAELVSQTGYGI c g t t t t c a c t a a a c c a a t c g g g g g c g c a g c c g a g c t g g t g a g t c a g a c a g g c t a c g g c a t g c a a a a g t g a t t t g g t t a g c c c c c g c g t c g g c t c g a c c a c t c a g t c t g t c c g a t g c c g t a

17,040 (in frame with EGFP) LHGAGLSQLPKQRHQPSDLH (in frame with EGFP) LHGAGLSQLPKQRHQPSDLH LHGAGLSQLPKQRHQPSDLH t c t g c a c g g c g c a g g c c t g t c t c a g c t g c c t a a a c a a c g g c a t c a g c c a t c t g a c c t g c a a g a c g t g c c g c g t c c g g a c a g a g t c g a c g g a t t t g t t g c c g t a g t c g g t a g a c t g g a c g t

17,100 (in frame with EGFP) ADQAPNSHVKYVWKMLQSLG (in frame with EGFP) ADQAPNSHVKYVWKMLQSLG ADQAPNSHVKYVWKMLQSLG c g c c g a c c a g g c t c c c a a c a g c c a c g t g a a g t a c g t g t g g a a g a t g c t g c a g a g c c t g g g g c g g c t g g t c c g a g g g t t g t c g g t g c a c t t c a t g c a c a c c t t c t a c g a c g t c t c g g a c c c

17,160 (in frame with EGFP) RPEVHMALDVVLVRGSGQEH (in frame with EGFP) RPEVHMALDVVLVRGSGQEH RPEVHMALDVVLVRGSGQEH c a g a c c t g a g g t g c a t a t g g c c c t g g a c g t g g t g c t c g t g c g g g g c a g c g g t c a g g a a c a g t c t g g a c t c c a c g t a t a c c g g g a c c t g c a c c a c g a g c a c g c c c c g t c g c c a g t c c t t g t

17,220 (in frame with EGFP) EGCLLLTSEVLFVVSVSEDT (in frame with EGFP) EGCLLLTSEVLFVVSVSEDT EGCLLLTSEVLFVVSVSEDT c g a g g g c t g c c t g c t g t t a a c c t c c g a g g t g c t g t t c g t c g t g a g c g t a t c g g a g g a t a c g c t c c c g a c g g a c g a c a a t t g g a g g c t c c a c g a c a a g c a g c a c t c g c a t a g c c t c c t a t g

17,280 (in frame with EGFP) QQQAFPVTEIDCAQDSKQNN (in frame with EGFP) QQQAFPVTEIDCAQDSKQNN QQQAFPVTEIDCAQDSKQNN c c a g c a g c a a g c t t t t c c t g t g a c c g a g a t c g a c t g c g c c c a g g a t t c t a a g c a g a a c a a g g t c g t c g t t c g a a a a g g a c a c t g g c t c t a g c t g a c g c g g g t c c t a a g a t t c g t c t t g t t

17,340 (in frame with EGFP) LLTVQLKQPRVACDVEVDGV (in frame with EGFP) LLTVQLKQPRVACDVEVDGV LLTVQLKQPRVACDVEVDGV c t t a c t g a c a g t g c a a c t g a a g c a g c c t a g a g t g g c c t g t g a c g t a g a a g t c g a c g g t g t g a a t g a c t g t c a c g t t g a c t t c g t c g g a t c t c a c c g g a c a c t g c a t c t t c a g c t g c c a c a

17,400 (in frame with EGFP) RERLSEQQYNRLVDYITKTS (in frame with EGFP) RERLSEQQYNRLVDYITKTS RERLSEQQYNRLVDYITKTS g a g a g a g a g a t t g t c c g a a c a g c a g t a c a a c c g g c t g g t g g a c t a c a t t a c c a a g a c g a g c t c t c t c t c t a a c a g g c t t g t c g t c a t g t t g g c c g a c c a c c t g a t g t a a t g g t t c t g c t c

17,460 (in frame with EGFP) CHLAPSCSSMQIPCPVVAAE (in frame with EGFP) CHLAPSCSSMQIPCPVVAAE CHLAPSCSSMQIPCPVVAAE c t g c c a c c t g g c c c c t a g c t g t a g c a g t a t g c a g a t c c c c t g t c c t g t t g t g g c c g c c g a g a c g g t g g a c c g g g g a t c g a c a t c g t c a t a c g t c t a g g g g a c a g g a c a a c a c c g g c g g c t

17,520 (in frame with EGFP) PPPSTVKTYHYLVDPHFAQV (in frame with EGFP) PPPSTVKTYHYLVDPHFAQV PPPSTVKTYHYLVDPHFAQV a c c c c c c c c c t c c a c g g t g a a g a c t t a c c a c t a t c t g g t c g a c c c a c a t t t c g c c c a g g t t g g g g g g g g g a g g t g c c a c t t c t g a a t g g t g a t a g a c c a g c t g g g t g t a a a g c g g g t c c a

AgeI 17,580 (in frame with EGFP) FLSKFTMVKNKALRKGFP* (in frame with EGFP) FLSKFTMVKNKALRKGFP* FLSKFTMVKNKALRKGFP* g t t c c t t t c c a a g t t c a c c a t g g t g a a a a a c a a g g c c c t c a g a a a g g g c t t t c c c t g a a c c a a g g a a a g g t t c a a g t g g t a c c a c t t t t t g t t c c g g g a g t c t t t c c c g a a a g g g a c t t g

17,640 WPRE c g g t t a g t a a t g a g t t t g a t a t c t c g a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g g c c a a t c a t t a c t c a a a c t a t a g a g c t g t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c

17,700 WPRE *H A C A A C G A G G A A A A T G C G A T A C WPRE-R M a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a

17,760 WPRE (in frame with Factor Xa site) RQIMSNSGTHSENEGGQIFG PLYHAIASRMAFIFSSLYKS g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g

17,820 WPRE (in frame with Factor Xa site) PQQRKILLQPGNDPLTAHHA WLLSLYEELWPVVRQRGVVC c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c

17,880 WPRE (in frame with Factor Xa site) SHKSVCGGSTPANGGGTLEK TVFADATPTGWGIATTCQLL c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a

17,940 WPRE (in frame with Factor Xa site) GPSESEGERNGRRFEDGGAK SGTFAFPLPIATAELIAACL t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a

18,000 WPRE (in frame with Factor Xa site) GAAPCPSPQQASVIGHHQRP ARCWTGARLLGTDNSVVLSG t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c

18,060 WPRE (in frame with Factor Xa site) LQRGKWPQEGTNGGPNQAPR KLTSFPWLLACVATWILRGT g a a g c t g a c g t c c t t t c c a t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c c t t c g a c t g c a g g a a a g g t a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g

SacII 18,120 WPRE (in frame with Factor Xa site) GEAVDR 1 RGEI Factor Xa site SFCYVPSALNPADLPSRGLL g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a

18,180 WPRE PALRPLPRLRLRPQTSRISL g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a

18,240 WPRE A C T T T G T G C C T T C C T C T G T T TK-pA-R WAASPPGNGGG* t t g g g c c g c c t c c c c g c c t g g a a a c g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a a a c c c g g c g g a g g g g c g g a c c t t t g c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t

18,300 HSV TK poly(A) signal c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g g t g t t g g g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c c a c a a c c

18,360 HSV TK poly(A) signal g t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g

18,420 g a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g c t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c

18,480 t t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c a g a a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g t c t

18,540 f1 ori t c t g c g c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c a g a c g c g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g

18,600 f1 ori G F1ori-R g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g

18,660 f1 ori A G G A A A G C G A A A G A A G G G A F1ori-R t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a

18,720 f1 ori a a a t c g g g g c a t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a t t t a g c c c c g t a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t

18,780 f1 ori a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g

3ʹ 5ʹ 18,817 f1 ori G T G G A C T C F1ori-F t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.