Addgene pR-EGFP - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pR-EGFP

Map View

600 400 200 End (717) BsrGI (710) EGFP-C (654 .. 675) BlpI (622) BsiHKAI (610) BmrI (606) Bpu10I (604) MflI * - BstYI (500) EXFP-R (307 .. 326) PfoI * (281) BtsI - BtsαI (214) BssSI - BssSαI (181) BsrFI (151) BtgZI (122) EGFP-N (46 .. 67) BanI (36) BseRI (31) Start (0) mEGFP pR-EGFP 717 bp

Sequence View

BanIBseRIStart (0) 5ʹ 3ʹ 60 1 M 1a V 5 10 15 SKGEELFTGVVPILVELD mEGFP G A C C A G C T C G A C C T G EGFP-N MVSKGEELFTGVVPILVELD GEQGRGAVHRGGAHPGRAG HHALALLQEGPHHGDQDLQV a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g

120 20 25 30 35 GDVNGHKFSVSGEGEGDATY mEGFP C C G C T G C EGFP-N GDVNGHKFSVSGEGEGDATY RRRKRPQVQRVRRGRGRCHL AVYVAVLEAHGALALAIGGV g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g

BsrFIBtgZI 180 40 45 50 55 GKLTLKFICTTGKLPVPWPT mEGFP GKLTLKFICTTGKLPVPWPT RQADPEVHLHHRQAARALAH ALQGQLEDAGGALQGHGPGG g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g

BtsαIBtsIBssSαIBssSI 240 60 65 70 75 LVTTLTYGVQCFSRYPDHMK mEGFP LVTTLTYGVQCFSRYPDHMK PRDHPDLRRAVLQPLPRPHE EHGGQGVAHLAEAAVGVVHL c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c

PfoI* 300 80 85 90 95 QHDFFKSAMPEGYVQERTIF mEGFP QHDFFKSAMPEGYVQERTIF AARLLQVRHARRLRPGAHHL LVVEELGGHGFAVDLLAGDE c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g

360 100 105 110 115 FKDDGNYKTRAEVKFEGDTL mEGFP C T G C T G C C G T T G A T G T T C T G EXFP-R FKDDGNYKTRAEVKFEGDTL LQGRRQLQDPRRGEVRGRHP ELVVAVVLGAGLHLELAVGQ t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c

420 120 125 130 135 VNRIELKGIDFKEDGNILGH mEGFP VNRIELKGIDFKEDGNILGH GEPHRAEGHRLQGGRQHPGA HVADLQLADVELLVAVDQPV g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g

480 140 145 150 155 KLEYNYNSHNVYIMADKQKN mEGFP KLEYNYNSHNVYIMADKQKN QAGVQLQQPQRLYHGRQAEE LQLVVVVAVVDIDHGVLLLV a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g

BstYIMflI* 540 160 165 170 175 GIKVNFKIRHNIEDGSVQLA mEGFP GIKVNFKIRHNIEDGSVQLA RHQGELQDPPQHRGRQRAAR ADLHVELDAVVDLVAAHLEG g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g

600 180 185 190 195 DHYQQNTPIGDGPVLLPDNH mEGFP DHYQQNTPIGDGPVLLPDNH RPLPAEHPHRRRPRAAARQP VVVLLVGGDAVAGHQQGVVV g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g

BlpIBpu10IBmrIBsiHKAI 660 200 205 210 215 YLSTQSKLSKDPNEKRDHMV mEGFP C A T G G T C EGFP-C YLSTQSKLSKDPNEKRDHMV LPEHPVQAEQRPQREARSHG VQAGLGLQAFVGVLLAIVHD t a c c t g a g c a c c c a g t c c a a g c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c a t g g a c t c g t g g g t c a g g t t c g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g

End (717) BsrGI 3ʹ 5ʹ 717 220 225 230 235 LLEFVTAAGITLGMDELYK mEGFP C T G C T G G A G T T C G T G EGFP-C LLEFVTAAGITLGMDELYK PAGVRDRRRDHSRHGRAVQ QQLEHGGGPDSEAHVLQVL c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.