Addgene pcs2-GFP-IRES-mCherry Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pcs2-GFP-IRES-mCherry Sequencing Result


Map View

BspEI (6005) BmgBI (5973) IRES-F (5824 .. 5843) BfuAI - BspMI - PaqCI (5769) PmlI (5746) IRES reverse (5597 .. 5614) AvrII (5581) PpuMI (5332) EcoNI (5327) PfoI * (4983) Bsu36I (4971) PshAI (4679) BamHI (4652) SP6 (4609 .. 4626) NdeI (4140) SalI (3590) F1ori-R (3413 .. 3432) NaeI (3391) NgoMIV (3389) F1ori-F (3201 .. 3222) mCherry-R (31 .. 49) PstI - SbfI (241) BbvCI (424) SgrAI (567) XcmI (572) SnaBI (622) EBV-rev (650 .. 669) SV40pA-R (704 .. 723) HpaI (760) MfeI (769) EagI - NotI (828) SacII (834) BstXI - NsiI (846) T3 (873 .. 893) M13 rev M13 Reverse (912 .. 928) lac operator M13/pUC Reverse (925 .. 947) CAP binding site BspQI - SapI (1137) L4440 (1143 .. 1160) pBR322ori-F (1394 .. 1413) Amp-R (2696 .. 2715) pcs2-GFP-IRES-mCherry 6125 bp

Sequence View

5ʹ 3ʹ 60 40 45 50 55 EGRPYEGTQTAKLKVTKGGP mCherry G G T T C G A C T T C C A C T G G T T mCherry-R GRPYEGTQTAKLKVTKGGP a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g

120 60 65 70 75 LPFAWDILSPQFMYGSKAYV mCherry LPFAWDILSPQFMYGSKAYV t g c c c t t c g c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g a a c g g g a a g c g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c t

180 80 85 90 95 KHPADIPDYLKLSFPEGFKW mCherry KHPADIPDYLKLSFPEGFKW a g c a c c c c g c c g a c a t c c c c g a c t a c t t g a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g g t c g t g g g g c g g c t g t a g g g g c t g a t g a a c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c c

240 100 105 110 115 ERVMNFEDGGVVTVTQDSSL mCherry ERVMNFEDGGVVTVTQDSSL a g c g c g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c c t c c c t g c t c g c g c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g g a g g g a c g

SbfIPstI 300 120 125 130 135 QDGEFIYKVKLRGTNFPSDG mCherry QDGEFIYKVKLRGTNFPSDG a g g a c g g c g a g t t c a t c t a c a a g g t g a a g c t g c g c g g c a c c a a c t t c c c c t c c g a c g g c c t c c t g c c g c t c a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g t t g a a g g g g a g g c t g c c g g

360 140 145 150 155 PVMQKKTMGWEASSERMYPE mCherry PVMQKKTMGWEASSERMYPE c c g t a a t g c a g a a g a a a a c c a t g g g c t g g g a g g c c t c c t c c g a g c g g a t g t a c c c c g a g g g g c a t t a c g t c t t c t t t t g g t a c c c g a c c c t c c g g a g g a g g c t c g c c t a c a t g g g g c t c c

420 160 165 170 175 DGALKGEIKQRLKLKDGGHY mCherry DGALKGEIKQRLKLKDGGHY a c g g c g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g a a g g a c g g c g g c c a c t a c g t g c c g c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c t t c c t g c c g c c g g t g a t g c

BbvCI 480 180 185 190 195 DAEVKTTYKAKKPVQLPGAY mCherry DAEVKTTYKAKKPVQLPGAY a c g c t g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c a t g c g a c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g t

540 200 205 210 215 NVNIKLDITSHNEDYTIVEQ mCherry NVNIKLDITSHNEDYTIVEQ a c g t c a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t t g c a g t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a

XcmISgrAI 600 220 225 230 235 YERAEGRHSTGGMDELYK* mCherry YERAEGRHSTGGMDELYK* a c g a a c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g t g a t c t a t g c t t g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c a c t a g a t

SnaBI 660 SV40 poly(A) signal C T A C T C A A A C C EBV-rev g a a c t a t a g t g a g t c g t a t t a c g t a g a t c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g c t t g a t a t c a c t c a g c a t a a t g c a t c t a g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c

720 SV40 poly(A) signal G A A A T T T G T G A T G C T A T SV40pA-R T G T T T G G T G EBV-rev a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a

MfeIHpaI 780 SV40 poly(A) signal T G C SV40pA-R t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t

SacIIEagINotI 840 t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t a a t t c g c g g c c g c g g c g c c a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a a t t a a g c g c c g g c g c c g c g g

BstXINsiI 900 T3 promoter G G A A A T C A C T C C C A A T T A A C G T3 a a t g c a t t g g g c c c g g t a c c c a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t g c g c g c t t g t t a c g t a a c c c g g g c c a t g g g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a c g c g c g a a c

960 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g

1020 -10 -35 lac promoter CAP binding site a a c a t a c g a g c c g g g a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c t t g t a t g c t c g g c c c t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g

1080 CAP binding site a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c

BspQISapI 1140 c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a

1200 G A G C G A G T G A C T G A G C G A L4440 t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g

1260 t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t

1320 ori g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a

1380 ori a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g

1440 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a

1500 ori g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c

1560 ori c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c

1620 ori g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a

1680 ori c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c

1740 ori a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g

1800 ori g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t

1860 ori a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a

1920 ori g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a

1980 t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t

2040 t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g

2100 285 280 *WHKILSAG AmpR *WHKILSAG t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a

2160 275 270 265 260 IEAIQRNREDMTAQSGTTYI AmpR IEAIQRNREDMTAQSGTTYI a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t

2220 255 250 245 240 VVIRSPKGDPGLAAIIGRSG AmpR VVIRSPKGDPGLAAIIGRSG a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t

2280 235 230 225 220 REGAGSKDAIFWGAPLASRL AmpR REGAGSKDAIFWGAPLASRL c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t

2340 215 210 205 200 LPGAVKDAEMWDILQQRSAL AmpR LPGAVKDAEMWDILQQRSAL a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t

2400 195 190 185 180 TLLEGTLLKRLTTAMAVPMT AmpR TLLEGTLLKRLTTAMAVPMT g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c

2460 175 170 165 160 TDREDNPIAENLEPEWRDLR AmpR TDREDNPIAENLEPEWRDLR g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t

2520 155 150 145 140 TVHDGMNHLFATLEKPGGIT AmpR TVHDGMNHLFATLEKPGGIT g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a

2580 135 130 125 120 TLLLNAATNDSMTIAASCLE AmpR TLLLNAATNDSMTIAASCLE g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a

2640 115 110 105 100 RVTMGDTLHKETVPSYEVLD AmpR RVTMGDTLHKETVPSYEVLD c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t

2700 95 90 85 80 NQSYHIRRGLQEQGADIRSL AmpR A T A A T Amp-R NQSYHIRRGLQEQGADIRSL t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a

2760 75 70 65 60 VAGCLLVKFTSMMPFREEPR AmpR A C C G C G C C A C A T A G C Amp-R VAGCLLVKFTSMMPFREEPR a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t

2820 55 50 45 40 FSELIKGSNLDLEIYGVRAG AmpR FSELIKGSNLDLEIYGVRAG a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g

2880 35 30 25 LQDEADKVKVLTEPH 20 signal sequence AFVPL AmpR LQDEADKVKVLTEPHAFVPL a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c

2940 15 10 5 1 signal sequence CFAAFFPILAVRFHQISM AmpR AmpR promoter CFAAFFPILAVRFHQISM c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g

3000 AmpR promoter c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a

3060 AmpR promoter g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t

3120 f1 ori c c t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t g g a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a

3180 f1 ori c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c

3240 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a

3300 f1 ori c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g

3360 f1 ori c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t

NgoMIVNaeI 3420 f1 ori A G G G A A G A F1ori-R g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t

3480 f1 ori A A G C G A A A G G A G F1ori-R a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t

3540 f1 ori MRRYRARPIRHSGC c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c c c a t t c g c c a t t c a g g c t g c g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g g g t a a g c g g t a a g t c c g a c g

SalI 3600 CMV IE94 promoter ATVGKGDRCGPLRYYASRPP g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a c g c c a g t c g a c c g c c a c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t g c g g t c a g c t g g c g g t

3660 CMV IE94 promoter IQYGVYGLMPIQYGGSGPVP a t t c a a t a t g g c g t a t a t g g a c t c a t g c c a a t t c a a t a t g g t g g a t c t g g a c c t g t g c c a t a a g t t a t a c c g c a t a t a c c t g a g t a c g g t t a a g t t a t a c c a c c t a g a c c t g g a c a c g g t

3720 CMV IE94 promoter IQYGVYGLVPIQYGGSGPQP a t t c a a t a t g g c g t a t a t g g a c t c g t g c c a a t t c a a t a t g g t g g a t c t g g a c c c c a g c c a t a a g t t a t a c c g c a t a t a c c t g a g c a c g g t t a a g t t a t a c c a c c t a g a c c t g g g g t c g g t

3780 CMV IE94 promoter IQYGGLGTMPIQYGGPGTVP a t t c a a t a t g g c g g a c t t g g c a c c a t g c c a a t t c a a t a t g g c g g a c c t g g c a c t g t g c c a t a a g t t a t a c c g c c t g a a c c g t g g t a c g g t t a a g t t a t a c c g c c t g g a c c g t g a c a c g g t

3840 CMV IE94 promoter TGEGSTWHGAKFEEGSWPCA a c t g g g g a g g g g t c t a c t t g g c a c g g t g c c a a g t t t g a g g a g g g g t c t t g g c c c t g t g c c t g a c c c c t c c c c a g a t g a a c c g t g c c a c g g t t c a a a c t c c t c c c c a g a a c c g g g a c a c g g

3900 CMV IE94 promoter KSAILNWHGANNGGHIGYMP a a g t c c g c c a t a t t g a a t t g g c a t g g t g c c a a t a a t g g c g g c c a t a t t g g c t a t a t g c c a t t c a g g c g g t a t a a c t t a a c c g t a c c a c g g t t a t t a c c g c c g g t a t a a c c g a t a t a c g g t

3960 CMV IE94 promoter GSIYRQYPIWPYANMAIGQV g g a t c a a t a t a t a g g c a a t a t c c a a t a t g g c c c t a t g c c a a t a t g g c t a t t g g c c a g g t t c c t a g t t a t a t a t c c g t t a t a g g t t a t a c c g g g a t a c g g t t a t a c c g a t a a c c g g t c c a a

4020 CMV IE94 promoter QYYVLALCHIVFHIWVFLLT c a a t a c t a t g t a t t g g c c c t a t g c c a t a t a g t a t t c c a t a t a t g g g t t t t c c t a t t g a c g g t t a t g a t a c a t a a c c g g g a t a c g g t a t a t c a t a a g g t a t a t a c c c a a a a g g a t a a c t g c

4080 CMV IE94 promoter * t a g a t a g c c c c t c c c a a t g g g c g g t c c c a t a t a c c a t a t a t g g g g c t t c c t a a t a c c g c c a t c t a t c g g g g a g g g t t a c c c g c c a g g g t a t a t g g t a t a t a c c c c g a a g g a t t a t g g c g g

4140 CMV IE94 promoter c a t a g c c a c t c c c c c a t t g a c g t c a a t g g t c t c t a t a t a t g g t c t t t c c t a t t g a c g t c a g t a t c g g t g a g g g g g t a a c t g c a g t t a c c a g a g a t a t a t a c c a g a a a g g a t a a c t g c a g t

NdeI 4200 CMV IE94 promoter t a t g g g c g g t c c t a t t g a c g t a t a t g g c g c c t c c c c c a t t g a c g t c a a t t a c g g t a a a t g a t a c c c g c c a g g a t a a c t g c a t a t a c c g c g g a g g g g g t a a c t g c a g t t a a t g c c a t t t a c

4260 CMV IE94 promoter g c c c g c c t g g c t c a a t g c c c a t t g a c g t c a a t a g g a c c a c c c a c c a t t g a c g t c a a t g g g c g g g c g g a c c g a g t t a c g g g t a a c t g c a g t t a t c c t g g t g g g t g g t a a c t g c a g t t a c c c

4320 CMV IE94 promoter a t g g c t c a t t g c c c a t t c a t a t c c g t t c t c a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t a c c g a g t a a c g g g t a a g t a t a g g c a a g a g t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t

4380 CMV IE94 promoter t g g c c c a c t t g g c a g t a c a t c a a t a t c t a t t a a t a g t a a c t t g g c a a g t a c a t t a c t a t t a c c g g g t g a a c c g t c a t g t a g t t a t a g a t a a t t a t c a t t g a a c c g t t c a t g t a a t g a t a a

4440 CMV IE94 promoter g g a a g t a c g c c a g g g t a c a t t g g c a g t a c t c c c a t t g a c g t c a a t g g c g g t a a a t g g c c c c c t t c a t g c g g t c c c a t g t a a c c g t c a t g a g g g t a a c t g c a g t t a c c g c c a t t t a c c g g g

4500 CMV IE94 promoter g c g a t g g c t g c c a a g t a c a t c c c c a t t g a c g t c a a t g g g g a g g g g c a a t g a c g c a a a t g g c g c t a c c g a c g g t t c a t g t a g g g g t a a c t g c a g t t a c c c c t c c c c g t t a c t g c g t t t a c c

4560 CMV IE94 promoter g c g t t c c a t t g a c g t a a a t g g g c g g t a g g c g t g c c t a a t g g g a g g t c t a t a t a a g c a a t g c g c a a g g t a a c t g c a t t t a c c c g c c a t c c g c a c g g a t t a c c c t c c a g a t a t a t t c g t t a c

4620 CMV IE94 promoter SP6 promoter A T T T A G G T G A C A SP6 c t c g t t t a g g g a a c c g c c a t t c t g c c t g g g g a c g t c g g a g c a a g c t t g a t t t a g g t g a c a g a g c a a a t c c c t t g g c g g t a a g a c g g a c c c c t g c a g c c t c g t t c g a a c t a a a t c c a c t g t

PshAIBamHI 4680 SP6 promoter Kozak sequence C T A T A G SP6 c t a t a g a a t a c a a g c t a c t t g t t c t t t t t g c g g a t c c c g c c a c c a t g g g a g a c g g a c g a a g a t a t c t t a t g t t c g a t g a a c a a g a a a a a c g c c t a g g g c g g t g g t a c c c t c t g c c t g c t t

4740 1 5 10 MVSKGAELFTGIV AcGFP1 MVSKGAELFTGIV c g t c t c t g g a g g c g g a g g c a g c a t g g t g a g c a a g g g c g c c g a g c t g t t c a c c g g c a t c g t g c a g a g a c c t c c g c c t c c g t c g t a c c a c t c g t t c c c g c g g c t c g a c a a g t g g c c g t a g c a

4800 15 20 25 30 PILIELNGDVNGHKFSVSGE AcGFP1 PILIELNGDVNGHKFSVSGE g c c c a t c c t g a t c g a g c t g a a t g g c g a t g t g a a t g g c c a c a a g t t c a g c g t g a g c g g c g a c g g g t a g g a c t a g c t c g a c t t a c c g c t a c a c t t a c c g g t g t t c a a g t c g c a c t c g c c g c t

4860 35 40 45 50 GEGDATYGKLTLKFICTTGK AcGFP1 GEGDATYGKLTLKFICTTGK g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t

4920 55 60 65 70 LPVPWPTLVTTLSYGVQCFS AcGFP1 LPVPWPTLVTTLSYGVQCFS g c t g c c t g t g c c c t g g c c c a c c c t g g t g a c c a c c c t g a g c t a c g g c g t g c a g t g c t t c t c c g a c g g a c a c g g g a c c g g g t g g g a c c a c t g g t g g g a c t c g a t g c c g c a c g t c a c g a a g a g

Bsu36I 4980 75 80 85 90 RYPDHMKQHDFFKSAMPEGY AcGFP1 RYPDHMKQHDFFKSAMPEGY a c g c t a c c c c g a t c a c a t g a a g c a g c a c g a c t t c t t c a a g a g c g c c a t g c c t g a g g g c t a t g c g a t g g g g c t a g t g t a c t t c g t c g t g c t g a a g a a g t t c t c g c g g t a c g g a c t c c c g a t

PfoI* 5040 95 100 105 110 IQERTIFFEDDGNYKSRAEV AcGFP1 IQERTIFFEDDGNYKSRAEV c a t c c a g g a g c g c a c c a t c t t c t t c g a g g a t g a c g g c a a c t a c a a g t c g c g c g c c g a g g t g t a g g t c c t c g c g t g g t a g a a g a a g c t c c t a c t g c c g t t g a t g t t c a g c g c g c g g c t c c a

5100 115 120 125 130 KFEGDTLVNRIELTGTDFKE AcGFP1 KFEGDTLVNRIELTGTDFKE g a a g t t c g a g g g c g a t a c c c t g g t g a a t c g c a t c g a g c t g a c c g g c a c c g a t t t c a a g g a c t t c a a g c t c c c g c t a t g g g a c c a c t t a g c g t a g c t c g a c t g g c c g t g g c t a a a g t t c c t

5160 135 140 145 150 DGNILGNKMEYNYNAHNVYI AcGFP1 DGNILGNKMEYNYNAHNVYI g g a t g g c a a c a t c c t g g g c a a t a a g a t g g a g t a c a a c t a c a a c g c c c a c a a t g t g t a c a t c c t a c c g t t g t a g g a c c c g t t a t t c t a c c t c a t g t t g a t g t t g c g g g t g t t a c a c a t g t a

5220 155 160 165 170 MTDKAKNGIKVNFKIRHNIE AcGFP1 MTDKAKNGIKVNFKIRHNIE c a t g a c c g a c a a g g c c a a g a a t g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g t a c t g g c t g t t c c g g t t c t t a c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t

5280 175 180 185 190 DGSVQLADHYQQNTPIGDGP AcGFP1 DGSVQLADHYQQNTPIGDGP g g a t g g c a g c g t g c a g c t g g c c g a c c a c t a c c a g c a g a a t a c c c c c a t c g g c g a t g g c c c c c t a c c g t c g c a c g t c g a c c g g c t g g t g a t g g t c g t c t t a t g g g g g t a g c c g c t a c c g g g

PpuMIEcoNI 5340 195 200 205 210 VLLPDNHYLSTQSALSKDPN AcGFP1 VLLPDNHYLSTQSALSKDPN t g t g c t g c t g c c c g a t a a c c a c t a c c t g t c c a c c c a g a g c g c c c t g t c c a a g g a c c c c a a a c a c g a c g a c g g g c t a t t g g t g a t g g a c a g g t g g g t c t c g c g g g a c a g g t t c c t g g g g t t

5400 215 220 225 230 EKRDHMIYFGFVTAAAITHG AcGFP1 EKRDHMIYFGFVTAAAITHG c g a g a a g c g c g a t c a c a t g a t c t a c t t c g g c t t c g t g a c c g c c g c c g c c a t c a c c c a c g g g c t c t t c g c g c t a g t g t a c t a g a t g a a g c c g a a g c a c t g g c g g c g g c g g t a g t g g g t g c c

5460 235 240 MDELYK* AcGFP1 IRES MDELYK* c a t g g a t g a g c t g t a c a a g t g a t c t a g a g c c c c t c t c c c t c c c c c c c c c c t a a c g t t a c t g t a c c t a c t c g a c a t g t t c a c t a g a t c t c g g g g a g a g g g a g g g g g g g g g g a t t g c a a t g a

5520 IRES g g c c g a a g c c g c t t g g a a t a a g g c c g g t g t g c g t t t g t c t a t a t g t t a t t t t c c a c c a t a c c g g c t t c g g c g a a c c t t a t t c c g g c c a c a c g c a a a c a g a t a t a c a a t a a a a g g t g g t a t

5580 IRES t t g c c g t c t t t t g g c a a t g t g a g g g c c c g g a a a c c t g g c c c t g t c t t c t t g a c g a g c a t t a a c g g c a g a a a a c c g t t a c a c t c c c g g g c c t t t g g a c c g g g a c a g a a g a a c t g c t c g t a a

AvrII 5640 IRES G A G A G C G G T T T C C T T A C G IRES reverse c c t a g g g g t c t t t c c c c t c t c g c c a a a g g a a t g c a a g g t c t g t t g a a t g t c g t g a a g g a a g g a t c c c c a g a a a g g g g a g a g c g g t t t c c t t a c g t t c c a g a c a a c t t a c a g c a c t t c c t t

5700 IRES g c a g t t c c t c t g g a a g c t t c t t g a a g a c a a a c a a c g t c t g t a g c g a c c c t t t g c a g g c a g c g t c a a g g a g a c c t t c g a a g a a c t t c t g t t t g t t g c a g a c a t c g c t g g g a a a c g t c c g t c

PmlI 5760 IRES c g g a a c c c c c c a c c t g g c g a c a g g t g c c t c t g c g g c c a a a a g c c a c g t g t a t a a g a t a c a g c c t t g g g g g g t g g a c c g c t g t c c a c g g a g a c g c c g g t t t t c g g t g c a c a t a t t c t a t g t

BspMIPaqCIBfuAI 5820 IRES c c t g c a a a g g c g g c a c a a c c c c a g t g c c a c g t t g t g a g t t g g a t a g t t g t g g a a a g a g t c g g a c g t t t c c g c c g t g t t g g g g t c a c g g t g c a a c a c t c a a c c t a t c a a c a c c t t t c t c a g

5880 IRES T G G C T C T C C T C A A G C G T A T T IRES-F a a a t g g c t c t c c t c a a g c g t a t t c a a c a a g g g g c t g a a g g a t g c c c a g a a g g t a c c c c a t t t t a c c g a g a g g a g t t c g c a t a a g t t g t t c c c c g a c t t c c t a c g g g t c t t c c a t g g g g t a

5940 IRES t g t a t g g g a t c t g a t c t g g g g c c t c g g t g c a c a t g c t t t a c a t g t g t t t a g t c g a g g t t a a c a t a c c c t a g a c t a g a c c c c g g a g c c a c g t g t a c g a a a t g t a c a c a a a t c a g c t c c a a t

BmgBI 6000 IRES a a a a a a c g t c t a g g c c c c c c g a a c c a c g g g g a c g t g g t t t t c c t t t g a a a a a c a c g a t a a t t t t t t g c a g a t c c g g g g g g c t t g g t g c c c c t g c a c c a a a a g g a a a c t t t t t g t g c t a t t

BspEI 6060 1 5 10 15 MVSKGEEDNMAIIKEFM IRES mCherry MVSKGEEDNMAIIKEFM t a c c t c c g g a a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c c a t c a t c a a g g a g t t c a t a t g g a g g c c t t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g g t a g t a g t t c c t c a a g t a

6120 20 25 30 35 RFKVHMEGSVNGHEFEIEGE mCherry RFKVHMEGSVNGHEFEIEGE g c g c t t c a a g g t g c a c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a c g c g a a g t t c c a c g t g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t

3ʹ 5ʹ 6125 G mCherry G g g g c g c c c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.