Addgene pLV-SFFV-CALU-WPRE-UbC-Emerald Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pLV-SFFV-CALU-WPRE-UbC-Emerald Sequencing Result


Map View

PmlI (11,713) EBV-rev (11,213 .. 11,232) SV40pA-R (11,159 .. 11,178) HpaI (11,121) SV40 NLS ApaI (10,402) PspOMI (10,398) PshAI (10,163) SfiI (9286) pBABE 3' (9014 .. 9034) L4440 (8927 .. 8944) pBR322ori-F (8674 .. 8693) AhdI (7941) FspI (7718) PvuI (7572) Amp-R (7372 .. 7391) pBRforEco (6963 .. 6981) pGEX 3' (6903 .. 6925) pRS-marker (6784 .. 6803) pBRrevBam (6551 .. 6570) SgrAI (6523) SP6 (6244 .. 6261) SP6 promoter PaeR7I - PspXI - XhoI (5891) BspEI (11,731) NruI * (504) FseI (821) gp41 peptide EcoRI (1893) BstEII (2140) AscI (2364) PflFI - Tth111I (2386) Bsu36I (2987) BstXI * (3258) XcmI (3283) NotI (3393) SalI (3409) SbfI (3419) WPRE-R (3504 .. 3524) SacII (3954) PaqCI (4816) hUBCpro-F (4972 .. 4991) SpeI (5085) EGFP-N (5216 .. 5237) EXFP-R (5477 .. 5496) EGFP-C (5824 .. 5845) pLV-SFFV-CALU-WPRE-UbC-Emerald 11,752 bp

Sequence View

5ʹ 3ʹ 60 3' LTR t g a t a t c g a g c t t g c t a c a a g g g a c t t t c c g c t g g g g a c t t t c c a g g g a g g c g t g g c c t g a c t a t a g c t c g a a c g a t g t t c c c t g a a a g g c g a c c c c t g a a a g g t c c c t c c g c a c c g g a c

120 3' LTR g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a t c c t g c a t a t a a g c a g c t g c t t t t t g c c t g c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t a g g a c g t a t a t t c g t c g a c g a a a a a c g g a c

180 3' LTR t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t

240 3' LTR c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a

300 3' LTR g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g

360 3' LTR HIV-1 Ψ t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t

420 HIV-1 Ψ c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a

480 HIV-1 Ψ a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a

NruI* 540 a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c

600 a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a

660 a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t

720 *IMIYYLLLGRN t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a

780 HADFSLSLLCWPLKLCSLPL t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t

FseI 840 AFCFYSWRVALPRGSIKLGP g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g g c c g c t g a t c t t c a g a c c t g c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c c g g c g a c t a g a a g t c t g g a c

900 MRDNWRSELYKYKVVK PPSILSLQLLSNYLYLTTFI g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t

960 IEPLGVAPTKAKRRVVQREK SGNPTAGVLAFLLTTCLSFL t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t

1020 RRE RAVGIGALFLGFLGAAGSTM ATPIPAKNRPNKPAAPLVIP g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c

1080 RRE GAASMTLTVQARQLLSGIVQ AADIVSVTCALCNNDPITCC g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g

1140 RRE QQNNLLRAIEAQQHLLQLTV CFLKSLAISACCCRNCSVTQ a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a

1200 RRE WGIKQLQARILAVERYLKDQ PM g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g

1260 RRE QLLGIWGCSGKLICTTAVPW a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t

1320 NASWSNKSLEQIWNHTTWME a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a

1380 WDREINNYTSLIHSLIEESQ g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t

1440 1 5 10 15 KNEQELLELDKWASL W gp41 peptide NQQEKNEQELLELDKWASLW a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t

1500 (in frame with gp41 peptide) NWFNITNWLWYIKLFIMIVG NWFNITNWLWYIKLFIMIVG a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c

1560 (in frame with gp41 peptide) GLVGLRIVFAVLSIVNRVRQ GLVGLRIVFAVLSIVNRVRQ g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c

1620 (in frame with gp41 peptide) GYSPLSFQTHLPTPRGPDRP GYSPLSFQTHLPTPRGPDRP g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c

1680 (in frame with gp41 peptide) EGIEEEGGERDRDRSIRLVN EGIEEEGGERDRDRSIRLVN a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c

1740 (in frame with gp41 peptide) GSRRYRQMAVFIHNFKRKGG cPPT/CTS GSRRYRQMAVFIHNFKRKGG g a t c t c g a c g g t a t c g c c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a c t a g a g c t g c c a t a g c g g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t

1800 (in frame with gp41 peptide) IGGYSAGERIVDIIATDIQT cPPT/CTS IGGYSAGERIVDIIATDIQT t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t

1860 (in frame with gp41 peptide) KELQKQITKIQNFRVYYRDS cPPT/CTS KELQKQITKIQNFRVYYRDS a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t

EcoRI 1920 (in frame with gp41 peptide) RDPVWIDKLDIEFLQPR* RDPVWIDKLDIEFLQPR* g a g a t c c a g t t t g g a t c g a t a a g c t t g a t a t c g a a t t c c t g c a g c c c c g a t a a a a t a a a a c t c t a g g t c a a a c c t a g c t a t t c g a a c t a t a g c t t a a g g a c g t c g g g g c t a t t t t a t t t t

1980 g a t t t t a t t t a g t c t c c a g a a a a a g g g g g g a a t g a a a g a c c c c a c c t g t a g g t t t g g c a a c t a a a a t a a a t c a g a g g t c t t t t t c c c c c c t t a c t t t c t g g g g t g g a c a t c c a a a c c g t t

2040 SFFV promoter g c t a g c t g c a g t a a c g c c a t t t t g c a a g g c a t g g a a a a a t a c c a a a c c a a g a a t a g a g a a c g a t c g a c g t c a t t g c g g t a a a a c g t t c c g t a c c t t t t t a t g g t t t g g t t c t t a t c t c t t

2100 SFFV promoter g t t c a g a t c a a g g g c g g g t a c a t g a a a a t a g c t a a c g t t g g g c c a a a c a g g a t a t c t g c g c a a g t c t a g t t c c c g c c c a t g t a c t t t t a t c g a t t g c a a c c c g g t t t g t c c t a t a g a c g c

BstEII 2160 SFFV promoter g t g a g c a g t t t c g g c c c c g g c c c g g g g c c a a g a a c a g a t g g t c a c c g c a g t t t c g g c c c c c a c t c g t c a a a g c c g g g g c c g g g c c c c g g t t c t t g t c t a c c a g t g g c g t c a a a g c c g g g g

2220 SFFV promoter g g c c c g a g g c c a a g a a c a g a t g g t c c c c a g a t a t g g c c c a a c c c t c a g c a g t t t c t t a a g c c g g g c t c c g g t t c t t g t c t a c c a g g g g t c t a t a c c g g g t t g g g a g t c g t c a a a g a a t t c

2280 SFFV promoter a c c c a t c a g a t g t t t c c a g g c t c c c c c a a g g a c c t g a a a t g a c c c t g c g c c t t a t t t g a a t g g g t a g t c t a c a a a g g t c c g a g g g g g t t c c t g g a c t t t a c t g g g a c g c g g a a t a a a c t t

2340 SFFV promoter t t a a c c a a t c a g c c t g c t t c t c g c t t c t g t t c g c g c g c t t c t g c t t c c c g a g c t c t a t a a a a t t g g t t a g t c g g a c g a a g a g c g a a g a c a a g c g c g c g a a g a c g a a g g g c t c g a g a t a t t

Tth111IPflFIAscI 2400 SFFV promoter a a g a g c t c a c a a c c c c t c a c t c g g c g c g c c a g t c c t c c g a c a g a c t g a g t c g c c c g g g g g t t c t c g a g t g t t g g g g a g t g a g c c g c g c g g t c a g g a g g c t g t c t g a c t c a g c g g g c c c c c

2460 MKETDLIIMDLRQF g a t c c a c c g g t c g c c a c c a t g a a g g a a a c t g a t c t a a t t a t c a t g g a c c t g c g a c a g t t t c t a g g t g g c c a g c g g t g g t a c t t c c t t t g a c t a g a t t a a t a g t a c c t g g a c g c t g t c a a a

2520 LMCLSLCTAFALSKPTEKKD c t t a t g t g c c t g t c c c t g t g c a c a g c c t t t g c c t t g a g c a a a c c c a c a g a a a a g a a g g a c g a a t a c a c g g a c a g g g a c a c g t g t c g g a a a c g g a a c t c g t t t g g g t g t c t t t t c t t c c t g

2580 RVHHEPQLSDKVHNDAQSFD c g t g t a c a t c a t g a g c c t c a g c t c a g t g a c a a g g t t c a c a a t g a t g c t c a g a g t t t t g a t g c a c a t g t a g t a c t c g g a g t c g a g t c a c t g t t c c a a g t g t t a c t a c g a g t c t c a a a a c t a

2640 YDHDAFLGAEEAKTFDQLTP t a t g a c c a t g a t g c c t t c t t g g g t g c t g a a g a a g c a a a g a c c t t t g a t c a g c t g a c a c c a a t a c t g g t a c t a c g g a a g a a c c c a c g a c t t c t t c g t t t c t g g a a a c t a g t c g a c t g t g g t

2700 EESKERLGKIVSKIDGDKDG g a a g a g a g c a a g g a a a g g c t t g g a a a g a t t g t a a g t a a a a t a g a t g g c g a c a a g g a c g g g c t t c t c t c g t t c c t t t c c g a a c c t t t c t a a c a t t c a t t t t a t c t a c c g c t g t t c c t g c c c

2760 FVTVDELKDWIKFAQKRWIY t t t g t c a c t g t g g a t g a g c t c a a a g a c t g g a t t a a a t t t g c a c a a a a g c g c t g g a t t t a c a a a c a g t g a c a c c t a c t c g a g t t t c t g a c c t a a t t t a a a c g t g t t t t c g c g a c c t a a a t g

2820 EDVERQWKGHDLNEDGLVSW g a g g a t g t a g a g c g a c a g t g g a a g g g g c a t g a c c t c a a t g a g g a c g g c c t c g t t t c c t g g c t c c t a c a t c t c g c t g t c a c c t t c c c c g t a c t g g a g t t a c t c c t g c c g g a g c a a a g g a c c

2880 EEYKNATYGYVLDDPDPDDG g a g g a g t a t a a a a a t g c c a c c t a c g g c t a c g t t t t a g a t g a t c c a g a t c c t g a t g a t g g a c t c c t c a t a t t t t t a c g g t g g a t g c c g a t g c a a a a t c t a c t a g g t c t a g g a c t a c t a c c t

2940 FNYKQMMVRDERRFKMADKD t t t a a c t a t a a a c a g a t g a t g g t t a g a g a t g a g c g g a g g t t t a a a a t g g c a g a c a a g g a t a a a t t g a t a t t t g t c t a c t a c c a a t c t c t a c t c g c c t c c a a a t t t t a c c g t c t g t t c c t a

Bsu36I 3000 GDLIATKEEFTAFLHPEEYD g g a g a c c t c a t t g c c a c c a a g g a g g a g t t c a c a g c t t t c c t g c a c c c t g a g g a g t a t g a c c c t c t g g a g t a a c g g t g g t t c c t c c t c a a g t g t c g a a a g g a c g t g g g a c t c c t c a t a c t g

3060 YMKDIVVQETMEDIDKNADG t a c a t g a a a g a t a t a g t a g t a c a g g a a a c a a t g g a a g a t a t a g a t a a g a a t g c t g a t g g t a t g t a c t t t c t a t a t c a t c a t g t c c t t t g t t a c c t t c t a t a t c t a t t c t t a c g a c t a c c a

3120 FIDLEEYIGDMYSHDGNTDE t t c a t t g a t c t a g a a g a g t a t a t t g g t g a c a t g t a c a g c c a t g a t g g g a a t a c t g a t g a g a a g t a a c t a g a t c t t c t c a t a t a a c c a c t g t a c a t g t c g g t a c t a c c c t t a t g a c t a c t c

3180 PEWVKTEREQFVEFRDKNRD c c a g a a t g g g t a a a g a c a g a g c g a g a g c a g t t t g t t g a g t t t c g g g a t a a g a a c c g t g a t g g t c t t a c c c a t t t c t g t c t c g c t c t c g t c a a a c a a c t c a a a g c c c t a t t c t t g g c a c t a

3240 GKMDKEETKDWILPSDYDHA g g g a a g a t g g a c a a g g a a g a g a c c a a a g a c t g g a t c c t t c c c t c a g a c t a t g a t c a t g c a c c c t t c t a c c t g t t c c t t c t c t g g t t t c t g a c c t a g g a a g g g a g t c t g a t a c t a g t a c g t

XcmIBstXI* 3300 EAEARHLVYESDQNKDGKLT g a g g c a g a a g c c a g g c a c c t g g t c t a t g a a t c a g a c c a a a a c a a g g a t g g c a a g c t t a c c c t c c g t c t t c g g t c c g t g g a c c a g a t a c t t a g t c t g g t t t t g t t c c t a c c g t t c g a a t g g

3360 KEEIVDKYDLFVGSQATDFG a a g g a g g a g a t c g t t g a c a a g t a t g a c t t a t t t g t t g g c a g c c a g g c c a c a g a t t t t g g g t t c c t c c t c t a g c a a c t g t t c a t a c t g a a t a a a c a a c c g t c g g t c c g g t g t c t a a a a c c c

SbfISalINotI 3420 EALVRHDEF* g a g g c c t t a g t a c g g c a t g a t g a g t t c t g a a g c g g c c g c g a c t c t a g a g t c g a c c t g c a g c t c c g g a a t c a t g c c g t a c t a c t c a a g a c t t c g c c g g c g c t g a g a t c t c a g c t g g a c g t c

3480 g c a t g c a a g c t t g a t a t c a a g c t t a t c g a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a c g t a c g t t c g a a c t a t a g t t c g a a t a g c t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t

3540 A C A A C G A G G A A A A T G C G A T A C WPRE-R a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t

3600 MPLYHAIASRMAFIFSSLYK MLLLPVWLSFSPPCIN a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t

3660 SWLLSLYEELWPVVRQRGVV PGCCLFMRSCGPLSGNVAWC t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c

3720 CTVFADATPTGWGIATTCQL ALCLLTQPPLVGALPPPVSS t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g

3780 LSGTFAFPLPIATAELIAAC FPGLSLSPSLLPRRNSSPPA c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g

3840 LARCWTGARLLGTDNSVVLS LPAAGQGLGCWALTIPWCCR c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c

3900 (in frame with Factor Xa site) *RGKRPQEGTNGGPNQAP GKSSSFPWLLACVATWILRG GNHRPFLGCSPVLPPGFCAG g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c

SacII 3960 (in frame with Factor Xa site) RGEAVDR 1 RGEI Factor Xa site TSFCYVPSALNPADLPSRGR RPSATSLRPSIQRTFLPAGA a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g g c g c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c c g c g

4020 PPPHGERCHVRRRAQRAS* PLLTASAATSDEGRSERPDP c c c c c t c c t c a c g g c g a g c g c t g c c a c g t c a g a c g a a g g g c g c a g c g a g c g t c c t g a t c c g g g g g a g g a g t g c c g c t c g c g a c g g t g c a g t c t g c t t c c c g c g t c g c t c g c a g g a c t a g g

4080 SARTLRTAARCS* t t c c g c c c g g a c g c t c a g g a c a g c g g c c c g c t g c t c a t a a g a c t c g g c c t t a g a a c c c c a a a g g c g g g c c t g c g a g t c c t g t c g c c g g g c g a c g a g t a t t c t g a g c c g g a a t c t t g g g g t

4140 g t a t c a g c a g a a g g a c a t t t t a g g a c g g g a c t t g g g t g a c t c t a g g g c a c t g g t t t t c t t c a t a g t c g t c t t c c t g t a a a a t c c t g c c c t g a a c c c a c t g a g a t c c c g t g a c c a a a a g a a

4200 t c c a g a g a g c g g a a c a g g c g a g g a a a a g t a g t c c c t t c t c g g c g a t t c t g c g g a g g g a t c a g g t c t c t c g c c t t g t c c g c t c c t t t t c a t c a g g g a a g a g c c g c t a a g a c g c c t c c c t a g

4260 t c c g t g g g g c g g t g a a c g c c g a t g a t t a t a t a a g g a c g c g c c g g g t g t g g c a c a g c t a g t a g g c a c c c c g c c a c t t g c g g c t a c t a a t a t a t t c c t g c g c g g c c c a c a c c g t g t c g a t c a

4320 t c c g t c g c a g c c g g g a t t t g g g t c g c g g t t c t t g t t t g t g g a t c g c t g t g a t c g t c a c t t a g g c a g c g t c g g c c c t a a a c c c a g c g c c a a g a a c a a a c a c c t a g c g a c a c t a g c a g t g a a

4380 g g t g a g t a g c g g g c t g c t g g g c t g g c c g g g g c t t t c g t g g c c g c c g g g c c g c t c g g t g g g c c a c t c a t c g c c c g a c g a c c c g a c c g g c c c c g a a a g c a c c g g c g g c c c g g c g a g c c a c c c

4440 a c g g a a g c g t g t g g a g a g a c c g c c a a g g g c t g t a g t c t g g g t c c g c g a g c a a g g t t g c c c t g c c t t c g c a c a c c t c t c t g g c g g t t c c c g a c a t c a g a c c c a g g c g c t c g t t c c a a c g g g

4500 t g a a c t g g g g g t t g g g g g g a g c g c a g c a a a a t g g c g g c t g t t c c c g a g t c t t g a a t g g a a a c t t g a c c c c c a a c c c c c c t c g c g t c g t t t t a c c g c c g a c a a g g g c t c a g a a c t t a c c t t

4560 g a c g c t t g t g a g g c g g g c t g t g a g g t c g t t g a a a c a a g g t g g g g g g c a t g g t g g g c g g c a c t g c g a a c a c t c c g c c c g a c a c t c c a g c a a c t t t g t t c c a c c c c c c g t a c c a c c c g c c g t

4620 a g a a c c c a a g g t c t t g a g g c c t t c g c t a a t g c g g g a a a g c t c t t a t t c g g g t g a g a t g g g t c t t g g g t t c c a g a a c t c c g g a a g c g a t t a c g c c c t t t c g a g a a t a a g c c c a c t c t a c c c

4680 c t g g g g c a c c a t c t g g g g a c c c t g a c g t g a a g t t t g t c a c t g a c t g g a g a a c t c g g t t t g g a c c c c g t g g t a g a c c c c t g g g a c t g c a c t t c a a a c a g t g a c t g a c c t c t t g a g c c a a a c

4740 t c g t c t g t t g c g g g g g c g g c a g t t a t g g c g g t g c c g t t g g g c a g t g c a c c c g t a c c t t t g a g c a g a c a a c g c c c c c g c c g t c a a t a c c g c c a c g g c a a c c c g t c a c g t g g g c a t g g a a a c

4800 g g a g c g c g c g c c c t c g t c g t g t c g t g a c g t c a c c c g t t c t g t t g g c t t a t a a t g c a g g g t c c t c g c g c g c g g g a g c a g c a c a g c a c t g c a g t g g g c a a g a c a a c c g a a t a t t a c g t c c c a

PaqCI 4860 g g g g c c a c c t g c c g g t a g g t g t g c g g t a g g c t t t t c t c c g t c g c a g g a c g c a g g g t t c g g c c c c g g t g g a c g g c c a t c c a c a c g c c a t c c g a a a a g a g g c a g c g t c c t g c g t c c c a a g c c

4920 g c c t a g g g t a g g c t c t c c t g a a t c g a c a g g c g c c g g a c c t c t g g t g a g g g g a g g g a t a a g c g g a t c c c a t c c g a g a g g a c t t a g c t g t c c g c g g c c t g g a g a c c a c t c c c c t c c c t a t t c

4980 T G A A G C T C C hUBCpro-F t g a g g c g t c a g t t t c t t t g g t c g g t t t t a t g t a c c t a t c t t c t t a a g t a g c t g a a g c t c c a c t c c g c a g t c a a a g a a a c c a g c c a a a a t a c a t g g a t a g a a g a a t t c a t c g a c t t c g a g g

5040 G G T T T T G A A C T hUBCpro-F g g t t t t g a a c t a t g c g c t c g g g g t t g g c g a g t g t g t t t t g t g a a g t t t t t t a g g c a c c t t c c a a a a c t t g a t a c g c g a g c c c c a a c c g c t c a c a c a a a a c a c t t c a a a a a a t c c g t g g a a

SpeI 5100 t t g a a a t g t a a t c a t t t g g g t c a a t a t g t a a t t t t c a g t g t t a g a c t a g t a a a t t g t c c g a a c t t t a c a t t a g t a a a c c c a g t t a t a c a t t a a a a g t c a c a a t c t g a t c a t t t a a c a g g c

5160 c t a a a t t c t g g c c g t t t t t g g c t t t t t t g t t a g a c g a a g c t t g g t a c c g a g c t c g g a t c a g a t t t a a g a c c g g c a a a a a c c g a a a a a a c a a t c t g c t t c g a a c c a t g g c t c g a g c c t a g t

5220 1 M 1a V 5 10 15 SKGEELFTGVVPILV EmGFP Kozak sequence G A C C A EGFP-N MVSKGEELFTGVVPILV a t t c g c c a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t t a a g c g g t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a

5280 20 25 30 35 ELDGDVNGHKFSVSGEGEGD EmGFP G C T C G A C C T G C C G C T G C EGFP-N ELDGDVNGHKFSVSGEGEGD c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t

5340 40 45 50 55 ATYGKLTLKFICTTGKLPVP EmGFP ATYGKLTLKFICTTGKLPVP t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g

5400 60 65 70 75 WPTLVTTLTYGVQCFARYPD EmGFP WPTLVTTLTYGVQCFARYPD c t g g c c c a c c c t c g t g a c c a c c t t g a c c t a c g g c g t g c a g t g c t t c g c c c g c t a c c c c g a g a c c g g g t g g g a g c a c t g g t g g a a c t g g a t g c c g c a c g t c a c g a a g c g g g c g a t g g g g c t

5460 80 85 90 95 HMKQHDFFKSAMPEGYVQER EmGFP HMKQHDFFKSAMPEGYVQER c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c

5520 100 105 110 115 TIFFKDDGNYKTRAEVKFEG EmGFP C T G C T G C C G T T G A T G T T C T G EXFP-R TIFFKDDGNYKTRAEVKFEG c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c

5580 120 125 130 135 DTLVNRIELKGIDFKEDGNI EmGFP DTLVNRIELKGIDFKEDGNI c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a

5640 140 145 150 155 LGHKLEYNYNSHKVYITADK EmGFP LGHKLEYNYNSHKVYITADK c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a g g t c t a t a t c a c c g c c g a c a a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t c c a g a t a t a g t g g c g g c t g t t

5700 160 165 170 175 QKNGIKVNFKTRHNIEDGSV EmGFP QKNGIKVNFKTRHNIEDGSV g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a c c c g c c a c a a c a t c g a g g a c g g c a g c g t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t g g g c g g t g t t g t a g c t c c t g c c g t c g c a

5760 180 185 190 195 QLADHYQQNTPIGDGPVLLP EmGFP QLADHYQQNTPIGDGPVLLP g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g

5820 200 205 210 215 DNHYLSTQSALSKDPNEKRD EmGFP DNHYLSTQSALSKDPNEKRD c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t

5880 220 225 230 235 HMVLLEFVTAAGITLGMDEL EmGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C HMVLLEFVTAAGITLGMDEL t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a

PaeR7IPspXIXhoI 5940 YK* EmGFP YK* g t a c a a g t a a c t c g a g g g a a t t a a t t c g a g c t c g g t a c c t t t a a g a c c a a t g a c t t a c a a c a t g t t c a t t g a g c t c c c t t a a t t a a g c t c g a g c c a t g g a a a t t c t g g t t a c t g a a t g t t

6000 3' LTR ( Δ U3) g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t

6060 3' LTR ( Δ U3) c t c c c a a c g a a g a c a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t g a g g g t t g c t t c t g t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a

6120 3' LTR ( Δ U3) c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a

6180 3' LTR ( Δ U3) g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g

6240 3' LTR ( Δ U3) c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g c a t c t a g a a t t a a t t c c g t g t g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c g t a g a t c t t a a t t a a g g c a c a

6300 SP6 promoter G A T A T C A C A G T G G A T T T A SP6 a t t c t a t a g t g t c a c c t a a a t c g t a t g t g t a t g a t a c a t a a g g t t a t g t a t t a a t t g t a g t a a g a t a t c a c a g t g g a t t t a g c a t a c a c a t a c t a t g t a t t c c a a t a c a t a a t t a a c a t c

6360 c c g c g t t c t a a c g a c a a t a t g t a c a a g c c t a a t t g t g t a g c a t c t g g c t t a c t g a a g c a g g g c g c a a g a t t g c t g t t a t a c a t g t t c g g a t t a a c a c a t c g t a g a c c g a a t g a c t t c g t c

6420 a c c c t a t c a t c t c t c t c g t a a a c t g c c g t c a g a g t c g g t t t g g t t g g a c g a a c c t t c t g a t g g g a t a g t a g a g a g a g c a t t t g a c g g c a g t c t c a g c c a a a c c a a c c t g c t t g g a a g a c t

6480 g t t t c t g g t a a c g c c g t c c c g c a c c c g g a a a t g g t c a g c g a a c c a a t c a g c a g g g t c a t c c a a a g a c c a t t g c g g c a g g g c g t g g g c c t t t a c c a g t c g c t t g g t t a g t c g t c c c a g t a g

SgrAI 6540 g c t a g c c a g a t c c t c t a c g c c g g a c g c a t c g t g g c c g g c a t c a c c g g c g c c a c a g g t g c g c g a t c g g t c t a g g a g a t g c g g c c t g c g t a g c a c c g g c c g t a g t g g c c g c g g t g t c c a c g c

6600 G G A T A T A G C G G C T G T A G T G G pBRrevBam g t t g c t g g c g c c t a t a t c g c c g a c a t c a c c g a t g g g g a a g a t c g g g c t c g c c a c t t c g g g c a a c g a c c g c g g a t a t a g c g g c t g t a g t g g c t a c c c c t t c t a g c c c g a g c g g t g a a g c c c

6660 c t c a t g a g c g c t t g t t t c g g c g t g g g t a t g g t g g c a g g c c c c g t g g c c g g g g g a c t g t t g g a g t a c t c g c g a a c a a a g c c g c a c c c a t a c c a c c g t c c g g g g c a c c g g c c c c c t g a c a a c

6720 g g c g c c a t c t c c t t g c a t g c a c c a t t c c t t g c g g c g g c g g t g c t c a a c g g c c t c a a c c t a c c g c g g t a g a g g a a c g t a c g t g g t a a g g a a c g c c g c c g c c a c g a g t t g c c g g a g t t g g a t

6780 c t a c t g g g c t g c t t c c t a a t g c a g g a g t c g c a t a a g g g a g a g c g t c g a a t g g t g c a c t c t g a t g a c c c g a c g a a g g a t t a c g t c c t c a g c g t a t t c c c t c t c g c a g c t t a c c a c g t g a g a

6840 A T G T T A G A C G A G A C T A C G G C pRS-marker c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g

6900 t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a

6960 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t

7020 C G G A G C A C T A T G C G G A T A A pBRforEco g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g

7080 AmpR promoter g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a

7140 AmpR promoter a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c

7200 1 5 10 15 signal sequence MSIQHFRVALIPFFAA AmpR promoter AmpR MSIQHFRVALIPFFAA a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g

7260 20 signal sequence FCLPVFA 25 30 35 HPETLVKVKDAED AmpR FCLPVFAHPETLVKVKDAED a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t

7320 40 45 50 55 QLGARVGYIELDLNSGKILE AmpR QLGARVGYIELDLNSGKILE t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t

7380 60 65 70 75 SFRPEERFPMMSTFKVLLCG AmpR C G A T A C A C C Amp-R SFRPEERFPMMSTFKVLLCG g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c

7440 80 85 90 95 AVLSRIDAGQEQLGRRIHYS AmpR G C G C C A T A A T A Amp-R AVLSRIDAGQEQLGRRIHYS c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g

7500 100 105 110 115 QNDLVEYSPVTEKHLTDGMT AmpR QNDLVEYSPVTEKHLTDGMT t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g

7560 120 125 130 135 VRELCSAAITMSDNTAANLL AmpR VRELCSAAITMSDNTAANLL a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a

PvuI 7620 140 145 150 155 LTTIGGPKELTAFLHNMGDH AmpR LTTIGGPKELTAFLHNMGDH t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t

7680 160 165 170 175 VTRLDRWEPELNEAIPNDER AmpR VTRLDRWEPELNEAIPNDER t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c

FspI 7740 180 185 190 195 DTTMPVAMATTLRKLLTGEL AmpR DTTMPVAMATTLRKLLTGEL t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a

7800 200 205 210 215 LTLASRQQLIDWMEADKVAG AmpR LTLASRQQLIDWMEADKVAG a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c

7860 220 225 230 235 PLLRSALPAGWFIADKSGAG AmpR PLLRSALPAGWFIADKSGAG a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c

7920 240 245 250 255 ERGSRGIIAALGPDGKPSRI AmpR ERGSRGIIAALGPDGKPSRI t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a

AhdI 7980 260 265 270 275 VVIYTTGSQATMDERNRQIA AmpR VVIYTTGSQATMDERNRQIA c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g

8040 280 285 EIGASLIKHW* AmpR EIGASLIKHW* t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a

8100 a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a

8160 t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g

8220 ori c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a

8280 ori g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g

8340 ori t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a

8400 ori g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a

8460 ori g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t

8520 ori c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g

8580 ori a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c

8640 ori a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a

8700 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g

8760 ori t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c

8820 ori g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g

8880 t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g

8940 A G C G A G T C A G T G A G L4440 c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c

9000 C G A G L4440 c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t

9060 SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' t t a a t g c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a a a t t a c g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t

9120 SV40 promoter g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t

9180 SV40 promoter g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g

9240 SV40 promoter SV40 ori c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g

SfiI 9300 SV40 promoter SV40 ori c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t

9360 SV40 promoter SV40 ori t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t t g g a c a c a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a a c c t g t g

9420 a a g a c a g g c t t g c g a g a t a t g t t t g a g a a t a c c a c t t t a t c c c g c g t c a g g g a g a g g c a g t t c t g t c c g a a c g c t c t a t a c a a a c t c t t a t g g t g a a a t a g g g c g c a g t c c c t c t c c g t c

9480 t g c g t a a a a a g a c g c g g a c t c a t g t g a a a t a c t g g t t t t t a g t g c g c c a g a t c t c t a t a a a c g c a t t t t t c t g c g c c t g a g t a c a c t t t a t g a c c a a a a a t c a c g c g g t c t a g a g a t a t t

9540 t c t c g c g c a a c c t a t t t t c c c c t c g a a c a c t t t t t a a g c c g t a g a t a a a c a g g c t g g g a c a g a g c g c g t t g g a t a a a a g g g g a g c t t g t g a a a a a t t c g g c a t c t a t t t g t c c g a c c c t g

9600 a c t t c a c a t g a g c g a a a a a t a c a t c g t c a c c t g g g a c a t g t t g c a g a t c c a t g c a c g t a a t g a a g t g t a c t c g c t t t t t a t g t a g c a g t g g a c c c t g t a c a a c g t c t a g g t a c g t g c a t t

9660 a c t c g c a a g c c g a c t g a t g c c t t c t g a a c a a t g g a a a g g c a t t a t t g c c g t a a g c c g t g g t g a g c g t t c g g c t g a c t a c g g a a g a c t t g t t a c c t t t c c g t a a t a a c g g c a t t c g g c a c c

9720 c g g t c t g t a c c g g g t g c g t t a c t g g c g c g t g a a c t g g g t a t t c g t c a t g t c g a t a c c g t t g c c a g a c a t g g c c c a c g c a a t g a c c g c g c a c t t g a c c c a t a a g c a g t a c a g c t a t g g c a a

9780 t g t a t t t c c a g c t a c g a t c a c g a c a a c c a g c g c g a g c t t a a a g t g c t g a a a c g c g c a g a a a c a t a a a g g t c g a t g c t a g t g c t g t t g g t c g c g c t c g a a t t t c a c g a c t t t g c g c g t c t t

9840 g g c g a t g g c g a a g g c t t c a t c g t t a t t g a t g a c c t g g t g g a t a c c g g t g g t a c t g c g g t t c c g c t a c c g c t t c c g a a g t a g c a a t a a c t a c t g g a c c a c c t a t g g c c a c c a t g a c g c c a a

9900 g c g a t t c g t g a a a t g t a t c c a a a a g c g c a c t t t g t c a c c a t c t t c g c a a a a c c g g c t g g t c g c t a a g c a c t t t a c a t a g g t t t t c g c g t g a a a c a g t g g t a g a a g c g t t t t g g c c g a c c a

9960 c g t c c g c t g g t t g a t g a c t a t g t t g t t g a t a t c c c g c a a g a t a c c t g g a t t g a a c a g c c g g c a g g c g a c c a a c t a c t g a t a c a a c a a c t a t a g g g c g t t c t a t g g a c c t a a c t t g t c g g c

10,020 t g g g a t a t g g g c g t c g t a t t c g t c c c g c c a a t c t c c g g t c g c t a a t c t t t t c a a c g c c t g a c c c t a t a c c c g c a g c a t a a g c a g g g c g g t t a g a g g c c a g c g a t t a g a a a a g t t g c g g a c

10,080 g c a c t g c c g g g c g t t g t t c t t t t t a a c t t c a g g c g g g t t a c a a t a g t t t c c a g t a a g t a t c g t g a c g g c c c g c a a c a a g a a a a a t t g a a g t c c g c c c a a t g t t a t c a a a g g t c a t t c a t a

10,140 t c t g g a g g c t g c a t c c a t g a c a c a g g c a a a c c t g a g c g a a a c c c t g t t c a a a c c c c g c t t a g a c c t c c g a c g t a g g t a c t g t g t c c g t t t g g a c t c g c t t t g g g a c a a g t t t g g g g c g a a

PshAI 10,200 t a a a c a t c c t g a a a c c t c g a c g c t a g t c c g c c g c t t t a a t c a c g g c g c a c a a c c g c c t g t a t t t g t a g g a c t t t g g a g c t g c g a t c a g g c g g c g a a a t t a g t g c c g c g t g t t g g c g g a c a

10,260 g c a g t c g g c c c t t g a t g g t a a a a c c a t c c c t c a c t g g t a t c g c a t g a t t a a c c g t c t g a t c g t c a g c c g g g a a c t a c c a t t t t g g t a g g g a g t g a c c a t a g c g t a c t a a t t g g c a g a c t a

10,320 g t g g a t c t g g c g c g g c a t t g a c c c a c g c g a a a t c c t c g a c g t c c a g g c a c g t a t t g t g a t c a c c t a g a c c g c g c c g t a a c t g g g t g c g c t t t a g g a g c t g c a g g t c c g t g c a t a a c a c t a

10,380 g a g c g a t g c c g a a c g t a c c g a c g a t g a t t t a t a c g a t a c g g t g a t t g g c t a c c g t g g c g g c t c g c t a c g g c t t g c a t g g c t g c t a c t a a a t a t g c t a t g c c a c t a a c c g a t g g c a c c g c c

PspOMIApaI 10,440 c a a c t g g a t t t a t g a g t g g g c c c c g g a t c t t t g t g a a g g a a c c t t a c t t c t g t g g t g t g a g t t g a c c t a a a t a c t c a c c c g g g g c c t a g a a a c a c t t c c t t g g a a t g a a g a c a c c a c a c t

10,500 small t intron c a t a a t t g g a c a a a c t a c c t a c a g a g a t t t a a a g c t c t a a g g t a a a t a t a a a a t t t t t a a g t a t t a a c c t g t t t g a t g g a t g t c t c t a a a t t t c g a g a t t c c a t t t a t a t t t t a a a a a t t

10,560 small t intron g t g t a t a a t g t g t t a a a c t a c t g a t t c t a a t t g t t t g t g t a t t t t a g a t t c c a a c c t a t g c a c a t a t t a c a c a a t t t g a t g a c t a a g a t t a a c a a a c a c a t a a a a t c t a a g g t t g g a t a c

10,620 g a a c t g a t g a a t g g g a g c a g t g g t g g a a t g c c t t t a a t g a g g a a a a c c t g t t t t g c t c a g c t t g a c t a c t t a c c c t c g t c a c c a c c t t a c g g a a a t t a c t c c t t t t g g a c a a a a c g a g t c

10,680 P SV40 NLS MPSSDDEATADSQHSTPP a a g a a a t g c c a t c t a g t g a t g a t g a g g c t a c t g c t g a c t c t c a a c a t t c t a c t c c t c c a a t t c t t t a c g g t a g a t c a c t a c t a c t c c g a t g a c g a c t g a g a g t t g t a a g a t g a g g a g g t t

10,740 130 KKKRKV (in frame with SV40 NLS) EDPKDFPSELLSFL SV40 NLS KKKRKVEDPKDFPSELLSFL a a a a g a a g a g a a a g g t a g a a g a c c c c a a g g a c t t t c c t t c a g a a t t g c t a a g t t t t t t g a t t t t c t t c t c t t t c c a t c t t c t g g g g t t c c t g a a a g g a a g t c t t a a c g a t t c a a a a a a c t

10,800 (in frame with SV40 NLS) SHAVFSNRTLACFAIYTTKE SHAVFSNRTLACFAIYTTKE g t c a t g c t g t g t t t a g t a a t a g a a c t c t t g c t t g c t t t g c t a t t t a c a c c a c a a a g g a a a c a g t a c g a c a c a a a t c a t t a t c t t g a g a a c g a a c g a a a c g a t a a a t g t g g t g t t t c c t t t

10,860 (in frame with SV40 NLS) KAALLYKKIMEKYSVTFISR KAALLYKKIMEKYSVTFISR a a g c t g c a c t g c t a t a c a a g a a a a t t a t g g a a a a a t a t t c t g t a a c c t t t a t a a g t a g g c t t c g a c g t g a c g a t a t g t t c t t t t a a t a c c t t t t t a t a a g a c a t t g g a a a t a t t c a t c c g

10,920 (in frame with SV40 NLS) HNSYNHNILFFLTPHRHRVS HNSYNHNILFFLTPHRHRVS a t a a c a g t t a t a a t c a t a a c a t a c t g t t t t t t c t t a c t c c a c a c a g g c a t a g a g t g t c t g t a t t g t c a a t a t t a g t a t t g t a t g a c a a a a a a g a a t g a g g t g t g t c c g t a t c t c a c a g a c

10,980 (in frame with SV40 NLS) AINNYAQKLCTFSFLICKGV AINNYAQKLCTFSFLICKGV c t a t t a a t a a c t a t g c t c a a a a a t t g t g t a c c t t t a g c t t t t t a a t t t g t a a a g g g g t t a g a t a a t t a t t g a t a c g a g t t t t t a a c a c a t g g a a a t c g a a a a a t t a a a c a t t t c c c c a a t

11,040 (in frame with SV40 NLS) NKEYLMYSALTRDHNQPYHI NKEYLMYSALTRDHNQPYHI a t a a g g a a t a t t t g a t g t a t a g t g c c t t g a c t a g a g a t c a t a a t c a g c c a t a c c a c a t t t t a t t c c t t a t a a a c t a c a t a t c a c g g a a c t g a t c t c t a g t a t t a g t c g g t a t g g t g t a a a

11,100 (in frame with SV40 NLS) CRGFTCFKKPPTPPPEPET* CRGFTCFKKPPTPPPEPET* g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t

HpaI 11,160 SV40 poly(A) signal C G SV40pA-R a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g

11,220 SV40 poly(A) signal G T G G T T T G EBV-rev T T A T C G T A G T G T T T A A A G SV40pA-R a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c

11,280 SV40 poly(A) signal T C C A A A C T C A T C EBV-rev t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g a t c a a c t g g a t a a c t c a a g c t a a c c a a a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g t t g a c c t a t t g a g t t c g a t t g g t t

11,340 a a t c a t c c c a a a c t t c c c a c c c c a t a c c c t a t t a c c a c t g c c a a t t a c c t g t g g t t t c a t t t a g t a g g g t t t g a a g g g t g g g g t a t g g g a t a a t g g t g a c g g t t a a t g g a c a c c a a a g t a

11,400 t t a c t c t a a a c c t g t g a t t c c t c t g a a t t a t t t t c a t t t t a a a g a a a t t g t a t t t g t t a a a a t g a g a t t t g g a c a c t a a g g a g a c t t a a t a a a a g t a a a a t t t c t t t a a c a t a a a c a a t t

11,460 3' LTR a t a t g t a c t a c a a a c t t a g t a g t t g g a a g g g c t a a t t c a c t c c c a a a g a a g a c a a g a t a t t a t a c a t g a t g t t t g a a t c a t c a a c c t t c c c g a t t a a g t g a g g g t t t c t t c t g t t c t a t a

11,520 3' LTR c c t t g a t c t g t g g a t c t a c c a c a c a c a a g g c t a c t t c c c t g a t t a g c a g a a c t a c a c a c c g g a a c t a g a c a c c t a g a t g g t g t g t g t t c c g a t g a a g g g a c t a a t c g t c t t g a t g t g t g g

11,580 3' LTR a g g g c c a g g g g t c a g a t a t c c a c t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a t c c c g g t c c c c a g t c t a t a g g t g a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t

11,640 3' LTR g c c a g a t a a g g t a g a a g a g g c c a a t a a a g g a g a g a a c a c c a g c t t g t t a c a c c c t g t g a g c g g t c t a t t c c a t c t t c t c c g g t t a t t t c c t c t c t t g t g g t c g a a c a a t g t g g g a c a c t c

11,700 3' LTR c c t g c a t g g g a t g g a t g a c c c g g a g a g a g a a g t g t t a g a g t g g a g g t t t g a c a g c c g c c t g g a c g t a c c c t a c c t a c t g g g c c t c t c t c t t c a c a a t c t c a c c t c c a a a c t g t c g g c g g a

BspEIPmlI 3ʹ 5ʹ 11,752 3' LTR a g c a t t t c a t c a c g t g g c c c g a g a g c t g c a t c c g g a g t a c t t c a a g a a c t g c t c g t a a a g t a g t g c a c c g g g c t c t c g a c g t a g g c c t c a t g a a g t t c t t g a c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.