Addgene pD(Nt)_His6 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pD(Nt)_His6 Sequencing Result


Map View

PI-SceI (1850) AvrII (1817) BlpI (1795) BpuEI (1751) SmlI (1730) NruI (1663) BspDI - ClaI (1656) SpeI (1649) BstXI (1639) KpnI (1593) Acc65I (1589) SacII (1588) EagI - NotI (1582) SnaBI (1538) AseI (1373) PsiI (1329) HindIII (1294) BstAPI (1144) AflIII (1119) PspFI (1072) BseYI (1068) Bsu36I (1000) AgeI - BsrFI (950) BglII (1858) MmeI (73) CAT-R (198 .. 217) EcoRI (345) BsrDI (364) AclI (432) BpmI - Eco57MI (467) PasI (577) MscI (612) TatI (760) ScaI (762) BaeGI - Bme1580I - Bsp1286I (816) BstBI (876) PflFI - Tth111I (893) DrdI (896) pD(Nt)_His6 1863 bp

Sequence View

60 cat promoter g g g c c a a c t t t t g g c g a a a a t g a g a c g t t g a t c g g c a c g t a a g a g g g t c c a a c t t t c a c c c c c g g t t g a a a a c c g c t t t t a c t c t g c a a c t a g c c g t g c a t t c t c c c a g g t t g a a a g t g g

MmeI 120 cat promoter a t a a t g a a a t a a g a t c a c t a c c g g g c g t a t t t t t t g a g t t a t c g a g a t t t t c a g g a g c t a t a t t a c t t t a t t c t a g t g a t g g c c c g c a t a a a a a a c t c a a t a g c t c t a a a a g t c c t c g a t

180 1 5 10 15 MEKKITGYTTVDISQW cat promoter CmR MEKKITGYTTVDISQW a g g a a g c t a a a a t g g a g a a a a a a a t c a c t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c t c c t t c g a t t t t a c c t c t t t t t t t a g t g a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g

240 20 25 30 35 HRKEHFEAFQSVAQCTYNQT CmR C T C C G T A A A G T C A G T C A A C G CAT-R HRKEHFEAFQSVAQCTYNQT a t c g t a a a g a a c a t t t t g a g g c a t t t c a g t c a g t t g c t c a a t g t a c c t a t a a c c a g a c c g t a g c a t t t c t t g t a a a a c t c c g t a a a g t c a g t c a a c g a g t t a c a t g g a t a t t g g t c t g g c

300 40 45 50 55 VQLDITAFLKTVKKNKHKFY CmR VQLDITAFLKTVKKNKHKFY t t c a g c t g g a t a t t a c g g c c t t t t t a a a g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c a a g t c g a c c t a t a a t g c c g g a a a a a t t t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g

EcoRI 360 60 65 70 75 PAFIHILARLMNAHPEFRMA CmR PAFIHILARLMNAHPEFRMA c g g c c t t t a t t c a c a t t c t t g c c c g c c t g a t g a a t g c t c a t c c g g a a t t c c g t a t g g c a a g c c g g a a a t a a g t g t a a g a a c g g g c g g a c t a c t t a c g a g t a g g c c t t a a g g c a t a c c g t t

BsrDI 420 80 85 90 95 MKDGELVIWDSVHPCYTVFH CmR MKDGELVIWDSVHPCYTVFH t g a a a g a c g g t g a g c t g g t g a t a t g g g a t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a c t t t c t g c c a c t c g a c c a c t a t a c c c t a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c

Eco57MIBpmIAclI 480 100 105 110 115 EQTETFSSLWSEYHDDFRQF CmR EQTETFSSLWSEYHDDFRQF a g c a a a c t g a a a c g t t t t c a t c g c t c t g g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t c g t t t g a c t t t g c a a a a g t a g c g a g a c c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g

540 120 125 130 135 LHIYSQDVACYGENLAYFPK CmR LHIYSQDVACYGENLAYFPK t a c a c a t a t a t t c g c a a g a t g t g g c g t g t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g a t g t g t a t a t a a g c g t t c t a c a c c g c a c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c

PasI 600 140 145 150 155 GFIENMFFVSANPWVSFTSF CmR GFIENMFFVSANPWVSFTSF g g t t t a t t g a g a a t a t g t t t t t c g t c t c a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g c c a a a t a a c t c t t a t a c a a a a a g c a g a g t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c

MscI 660 160 165 170 175 DLNVANMDNFFAPVFTMGKY CmR DLNVANMDNFFAPVFTMGKY a t t t a a a c g t g g c c a a t a t g g a c a a c t t c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t t a a a t t t g c a c c g g t t a t a c c t g t t g a a g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a

720 180 185 190 195 YTQGDKVLMPLAIQVHHAVC CmR YTQGDKVLMPLAIQVHHAVC a t a c g c a a g g c g a c a a g g t g c t g a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t t t g t g t a t g c g t t c c g c t g t t c c a c g a c t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a a a c a c

TatIScaI 780 200 205 210 215 DGFHVGRMLNELQQYCDEWQ CmR DGFHVGRMLNELQQYCDEWQ a t g g c t t c c a t g t c g g c a g a a t g c t t a a t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g t a c c g a a g g t a c a g c c g t c t t a c g a a t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c

Bme1580IBsp1286IBaeGI 840 220 GGA* CmR GGA* g t g g g g c g t a a t t t t t t t a a g g c a g t t a t t g g t g c c c t t a a a c g c c t g g t t g c t a c g c c t c a c c c c g c a t t a a a a a a a t t c c g t c a a t a a c c a c g g g a a t t t g c g g a c c a a c g a t g c g g a

Tth111IPflFIDrdIBstBI 900 g a a t a a g t g a t a a t a a g c g g a t g a a t g g c a g a a a t t c g a a a g c a a a t t c g a c c c g g t c g t c t t a t t c a c t a t t a t t c g c c t a c t t a c c g t c t t t a a g c t t t c g t t t a a g c t g g g c c a g c a

BsrFIAgeI 960 c g g t t c a g g g c a g g g t c g t t a a a t a g c c g c t t a t g t c t a t t g c t g g t t t a c c g g t t t a t t g c c a a g t c c c g t c c c a g c a a t t t a t c g g c g a a t a c a g a t a a c g a c c a a a t g g c c a a a t a a

Bsu36I 1020 g a c t a c c g g a a g c a g t g t g a c c g t g t g c t t c t c a a a t g c c t g a g g c c a g t t t g c t c a g g c c t g a t g g c c t t c g t c a c a c t g g c a c a c g a a g a g t t t a c g g a c t c c g g t c a a a c g a g t c c g

BseYIPspFI 1080 t c t c c c c g t g g a g g t a a t a a t t g a c g a t a t g a t c a t t t a t t c t g c c t c c c a g c t g a c a t t a g a g g g g c a c c t c c a t t a t t a a c t g c t a t a c t a g t a a a t a a g a c g g a g g g t c g a c t g t a a

AflIII 1140 c a t c c g g g g t c a g c a c c g t t t c t g c g g a c t g g c t t t c t a c g t g t t c c g c t t c c t t t a g c a g t a g g c c c c a g t c g t g g c a a a g a c g c c t g a c c g a a a g a t g c a c a a g g c g a a g g a a a t c g t

BstAPI 1200 R6K γ ori g c c c t t g c g c c c t g a g t g c t t g c g g c a g c g t g a a g c t a a t t c c c a t g t c a g c c g t t a a g t c g g g a a c g c g g g a c t c a c g a a c g c c g t c g c a c t t c g a t t a a g g g t a c a g t c g g c a a t t c a

1260 R6K γ ori g t t c c t g t g t c a c t c a a a a t t g c t t t g a g a g g c t c t a a g g g c t t c t c a g t g c g t t a c a t c c a a g g a c a c a g t g a g t t t t a a c g a a a c t c t c c g a g a t t c c c g a a g a g t c a c g c a a t g t a g

HindIII 1320 R6K γ ori c c t g g c t t g t t g t c c a c a a c c g t t a a a c c t t a a a a g c t t t a a a a g c c t t a t a t a t t c t t t g g a c c g a a c a a c a g g t g t t g g c a a t t t g g a a t t t t c g a a a t t t t c g g a a t a t a t a a g a a a

AseIPsiI 1380 R6K γ ori t t t t t c t t a t a a a a c t t a a a a c c t t a g a g g c t a t t t a a g t t g c t g a t t t a t a t t a a t t t t a a a a a g a a t a t t t t g a a t t t t g g a a t c t c c g a t a a a t t c a a c g a c t a a a t a t a a t t a a a a

1440 R6K γ ori a t t g t t c a a a c a t g a g a g c t t a g t a c g t g a a a c a t g a g a g c t t a g t a c g t t a g c c a t g a g t a a c a a g t t t g t a c t c t c g a a t c a t g c a c t t t g t a c t c t c g a a t c a t g c a a t c g g t a c t c

1500 R6K γ ori a g c t t a g t a c g t t a g c c a t g a g g g t t t a g t t c g t t a a a c a t g a g a g c t t a g t a c g t t a a a t c g a a t c a t g c a a t c g g t a c t c c c a a a t c a a g c a a t t t g t a c t c t c g a a t c a t g c a a t t t

SnaBI 1560 R6K γ ori c a t g a g a g c t t a g t a c g t g a a a c a t g a g a g c t t a g t a c g t a c t a t c a a c a g g t t g a a c t g g t a c t c t c g a a t c a t g c a c t t t g t a c t c t c g a a t c a t g c a t g a t a g t t g t c c a a c t t g a c

EagINotISacIIAcc65IKpnI 1620 loxP c t g a t c a a c a g a t c c t c t a c g c g g c c g c g g t a c c a t a a c t t c g t a t a g c a t a c a t t a t a c g a c t a g t t g t c t a g g a g a t g c g c c g g c g c c a t g g t a t t g a a g c a t a t c g t a t g t a a t a t g

NruIBspDIClaISpeIBstXI 1680 loxP g a a g t t a t c t g c c a g g c a c a t g g g t t t t a c t a g t a t c g a t t c g c g a c c t a c t c c g g a a t a c t t c a a t a g a c g g t c c g t g t a c c c a a a a t g a t c a t a g c t a a g c g c t g g a t g a g g c c t t a t

SmlI 1740 1 5 HHHHHH (in frame with 6xHis) AMETHCLSL 6xHis t t a g g t c t c a g g c g c a c c a t c a c c a t c a c c a t g c c a t g g a g a c c c a c t g c t t g a g c c t a g a a t c c a g a g t c c g c g t g g t a g t g g t a g t g g t a c g g t a c c t c t g g g t g a c g a a c t c g g a t c

BlpIBpuEI 1800 (in frame with 6xHis) EDPAANKARKEAELAAVTAE a a g a t c c g g c t g c t a a c a a a g c c c g a a a g g a a g c t g a g t t g g c t g c t g t c a c c g c t g a g c t t c t a g g c c g a c g a t t g t t t c g g g c t t t c c t t c g a c t c a a c c g a c g a c a g t g g c g a c t c g

BglIIPI-SceIAvrII 1860 Q* a a t a a c t a t c a t a a c c c c t a g g t g c c a t t t c a t t a c c t c t t t c t c c g c a c c c g a c a t a g a t t a t t g a t a g t a t t g g g g a t c c a c g g t a a a g t a a t g g a g a a a g a g g c g t g g g c t g t a t c t

1863 t c t a g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.