Addgene CIRTS-YTHDF2-Malat1 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: CIRTS-YTHDF2-Malat1 Sequencing Result


Map View

AmpR promoter SspI (12,281) Amp-R (12,027 .. 12,046) ScaI (11,957) PvuI (11,847) AhdI (11,477) pBR322ori-F (10,725 .. 10,744) PciI (10,584) L4440 (10,474 .. 10,491) CAP binding site lac promoter M13/pUC Reverse (10,256 .. 10,278) lac operator M13 Reverse (10,243 .. 10,259) M13 rev BstZ17I (10,205) EBV-rev (10,164 .. 10,183) SV40pA-R (10,110 .. 10,129) FseI (9845) SgrAI (9683) MauBI (9605) EM7 promoter SV40pro-F (9368 .. 9387) pBABE 3' (9121 .. 9141) F1ori-F (8981 .. 9002) F1ori-R (8771 .. 8790) BGH-rev (8408 .. 8425) PmeI (8392) ApaI (8387) PspOMI (8383) BspEI (8189) pBluescriptKS (7681 .. 7697) PaeR7I - XhoI (7693) KS primer WPRE-R (7142 .. 7162) EcoRI (7065) LKO.1 5' (6916 .. 6935) hU6-F (6745 .. 6765) XbaI (6739) BsmBI - Esp3I (6428) SgrDI (1) pRS-marker (47 .. 66) MluI (231) SpeI (252) SnaBI (593) CMV-F (772 .. 792) KasI (1018) NarI (1019) SfoI (1020) PluTI (1022) NotI (1525) KflI (2311) PacI (2598) FspAI (3360) AfeI (3595) EGFP-N (3777 .. 3798) EXFP-R (4038 .. 4057) EGFP-C (4385 .. 4406) BlpI (5382) NheI (5715) BmtI (5719) BstEII (5801) BstXI (6137) CIRTS-YTHDF2-Malat1 12,397 bp

Sequence View

SgrDI 5ʹ 3ʹ 60 A T G T T A G A C G A G A C pRS-marker g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c

120 T A C G G C pRS-marker a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a

180 g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g

MluI 240 CMV enhancer t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g

SpeI 300 CMV enhancer a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a

360 CMV enhancer a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c

420 CMV enhancer a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a

480 CMV enhancer t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c

540 CMV enhancer t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g

SnaBI 600 CMV enhancer a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c

660 CMV enhancer CMV promoter t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a

720 CMV promoter t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g

780 CMV promoter C G C A A A T G G CMV-F a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c

840 CMV promoter 5' LTR (truncated) G C G G T A G G C G T G CMV-F g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g c g c g t t t t g c c t g t a c t g g g t c t c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c g c g c a a a a c g g a c a t g a c c c a g a

900 5' LTR (truncated) c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a

960 5' LTR (truncated) a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g

KasINarISfoI 1020 5' LTR (truncated) t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g

PluTI 1080 HIV-1 Ψ g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g

1140 HIV-1 Ψ g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t

1200 HIV-1 Ψ t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c

1260 g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t

1320 a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g

1380 t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t

1440 c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g

1500 a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t

NotI 1560 M a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c

1620 RDNWRSELYKYKVVKIEPLG a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t

1680 RRE VAPTKAKRRVVQREKRAVGI g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t

1740 RRE GALFLGFLGAAGSTMGAASM g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c

1800 RRE TLTVQARQLLSGIVQQQNNL a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c

1860 RRE LRAIEAQQHLLQLTVWGIKQ c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c

1920 RRE LQARILAVERYLKDQQLLGI c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a

1980 WGCSGKLICTTAVPWNASWS t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a

2040 NKSLEQIWNHTTWMEWDREI a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a

2100 1 K gp41 peptide NNYTSLIHSLIEESQNQQEK a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c

2160 5 10 15 NEQELLELDKWASL (in frame with gp41 peptide) WNWFNI gp41 peptide NEQELLELDKWASLWNWFNI a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t

2220 (in frame with gp41 peptide) TNWLWYIKLFIMIVGGLVGL TNWLWYIKLFIMIVGGLVGL a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t

2280 (in frame with gp41 peptide) RIVFAVLSIVNRVRQGYSPL RIVFAVLSIVNRVRQGYSPL a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t

KflI 2340 (in frame with gp41 peptide) SFQTHLPTPRGPDRPEGIEE SFQTHLPTPRGPDRPEGIEE t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t

2400 (in frame with gp41 peptide) EGGERDRDRSIRLVNGSALR EGGERDRDRSIRLVNGSALR g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c g g c a c t g c g t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g c c g t g a c g c a

2460 (in frame with gp41 peptide) APILQTNGSIHPQF* cPPT/CTS APILQTNGSIHPQF* g c g c c a a t t c t g c a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t c g c g g t t a a g a c g t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a

2520 cPPT/CTS t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t

2580 cPPT/CTS a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c

PacI 2640 a g a t c c a g t t t g g t t a a t t a a g g g t t t t g g c t a c g t c c a g a g c a g a g g a a t g a g g g c a t g t c t a g g t c a a a c c a a t t a a t t c c c a a a a c c g a t g c a g g t c t c g t c t c c t t a c t c c c g t a c

2700 t a g a c t a a a t a t g t t c g t g t g g a a g a g g c t g a a t a c a c a t c a g a g t t a g t g c t g c a g g a a a t c t g a t t t a t a c a a g c a c a c c t t c t c c g a c t t a t g t g t a g t c t c a a t c a c g a c g t c c t t

2760 a t g c t t c t g c a t t g c a t a c c c a g a g t t t c c t t g c t c a t c t g a g a g c a t g t g t t t t t t c c a t a c g a a g a c g t a a c g t a t g g g t c t c a a a g g a a c g a g t a g a c t c t c g t a c a c a a a a a a g g t

2820 g a t g t g t g t a c t t g t g t g a g a t t c t c t g g g t g t g t g t c a a t g t g t t g c c t g a a c g t g c a t c t a c a c a c a t g a a c a c a c t c t a a g a g a c c c a c a c a c a g t t a c a c a a c g g a c t t g c a c g t a

2880 t g c t c a a t a t g c t c a t g t g t g t t a c c c t g g g c t t g t a c a t c t a c a t a t a t a c c t g g a t g c a c g a g t t a t a c g a g t a c a c a c a a t g g g a c c c g a a c a t g t a g a t g t a t a t a t g g a c c t a c g

2940 c c g t g t g t t c t g t g a t g t a c a t a t a c c c t g t g t c a t t c c t t g t t t t t c t a t t t g t g t t a t g g c a c a c a a g a c a c t a c a t g t a t a t g g g a c a c a g t a a g g a a c a a a a a g a t a a a c a c a a t a

3000 t c c a t g t g t t c c t t c a g g c t c t c a c t a c c c a a g t g t c c a c c t c c g c c t g t c t g g t g a t g t a g g t a c a c a a g g a a g t c c g a g a g t g a t g g g t t c a c a g g t g g a g g c g g a c a g a c c a c t a c a

3060 t t a c g c t a c c c c g t g c t c t t t t c t t t g c c t g a c a g t g t t g t c g t g g a a g a c a t c t c g c c a a a t g c g a t g g g g c a c g a g a a a a g a a a c g g a c t g t c a c a a c a g c a c c t t c t g t a g a g c g g t

3120 g g a a c a c t g c a g t a a g g a g a a t t t c t a g t t t t a t g t t c c c c t c c g a g t a t g c t t c t a t c c c c t t g t g a c g t c a t t c c t c t t a a a g a t c a a a a t a c a a g g g g a g g c t c a t a c g a a g a t a g g

3180 c g a c c c t c a a c c c c a a a a t g c c t t c a g a g g t g a a a a t c a a c a c t g g a a a c a c a a g t a t c t g c t g g g a g t t g g g g t t t t a c g g a a g t c t c c a c t t t t a g t t g t g a c c t t t g t g t t c a t a g a

3240 g g g a a g g g t a a c a a t g c a a g t t a g c c t g a g g a t t t a g g a g g a g g c t g a a a a a c a g a g t a g c c c t t c c c a t t g t t a c g t t c a a t c g g a c t c c t a a a t c c t c c t c c g a c t t t t t g t c t c a t c

3300 g a g c c t t a c t a c g g g t c c a g a c c c t a c g g a c a a g a a c c c c c a c t c c c a c t c c c c a a a t t g c t c g g a a t g a t g c c c a g g t c t g g g a t g c c t g t t c t t g g g g g t g a g g g t g a g g g g t t t a a c

FspAI 3360 c g c a t t c c c t c c c c c a t c a g a g g g g g a g g g g a a g a g g a t g c a g c g c g g c g c g g c g c g t g c g c g t a a g g g a g g g g g t a g t c t c c c c c t c c c c t t c t c c t a c g t c g c g c c g c g c c g c g c a c g

3420 g c a c t g t c g g a t t t a g t a c c g c g g a c a g a g c c t t c g c c c c c g c t g c c g g c g c g c g c c a c c c g t g a c a g c c t a a a t c a t g g c g c c t g t c t c g g a a g c g g g g g c g a c g g c c g c g c g c g g t g g

3480 a c c t c c c c a g c a c c a a a g g c g g g c t g a c g t c a c t c t c c a g c c c t c c c c a a a c t c c c c t a c t g g a g g g g t c g t g g t t t c c g c c c g a c t g c a g t g a g a g g t c g g g a g g g g t t t g a g g g g a t g

3540 c t c a c c g c c t t g g t c g c g t c c g t g c a g c g g t g a g t c c a g t c g g g c c g c a c c a c a a g a g g t g a g t g g c g g a a c c a g c g c a g g c a c g t c g c c a c t c a g g t c a g c c c g g c g t g g t g t t c t c c a

AfeI 3600 g c a a g a t a g g g g g g t g c a g g c g c g a c c a t a c g c t c t g c g g c g g c a g a g c c t c a g c g c t g c c g t t c t a t c c c c c c a c g t c c g c g c t g g t a t g c g a g a c g c c g c c g t c t c g g a g t c g c g a c g

3660 c t c a g t c t g c a g c g g g c a g c a g a g g a g t c g c g t c g t g c c a g a g a g c g c c g c c g t g c t c c t g a g t c a g a c g t c g c c c g t c g t c t c c t c a g c g c a g c a c g g t c t c t c g c g g c g g c a c g a g g a

3720 g a g c c c c t t g c g c t c c g c c c c c g c g g c c c a c c g a c c c a c t g c c c c t t g g a t c c c c g g g t a c t c g g g g a a c g c g a g g c g g g g g c g c c g g g t g g c t g g g t g a c g g g g a a c c t a g g g g c c c a t

3780 1 M 1a V 5 10 15 SKGEELFTGVVPIL EGFP 1 M 1a V 5 10 15 SKGEELFTGVVPIL EGFP Kozak sequence G A C C EGFP-N MVSKGEELFTGVVPIL c c g g t g c c a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g g g c c a c g g t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c

3840 20 25 30 35 VELDGDVNGHKFSVSGEGEG EGFP 20 25 30 35 VELDGDVNGHKFSVSGEGEG EGFP A G C T C G A C C T G C C G C T G C EGFP-N VELDGDVNGHKFSVSGEGEG t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c

3900 40 45 50 55 DATYGKLTLKFICTTGKLPV EGFP 40 45 50 55 DATYGKLTLKFICTTGKLPV EGFP DATYGKLTLKFICTTGKLPV a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g

3960 60 65 70 75 PWPTLVTTLTYGVQCFSRYP EGFP 60 65 70 75 PWPTLVTTLTYGVQCFSRYP EGFP PWPTLVTTLTYGVQCFSRYP c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c

4020 80 85 90 95 DHMKQHDFFKSAMPEGYVQE EGFP 80 85 90 95 DHMKQHDFFKSAMPEGYVQE EGFP DHMKQHDFFKSAMPEGYVQE a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g

4080 100 105 110 115 RTIFFKDDGNYKTRAEVKFE EGFP 100 105 110 115 RTIFFKDDGNYKTRAEVKFE EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R RTIFFKDDGNYKTRAEVKFE g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c

4140 120 125 130 135 GDTLVNRIELKGIDFKEDGN EGFP 120 125 130 135 GDTLVNRIELKGIDFKEDGN EGFP GDTLVNRIELKGIDFKEDGN g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t

4200 140 145 150 155 ILGHKLEYNYNSHNVYIMAD EGFP 140 145 150 155 ILGHKLEYNYNSHNVYIMAD EGFP ILGHKLEYNYNSHNVYIMAD t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t

4260 160 165 170 175 KQKNGIKVNFKIRHNIEDGS EGFP 160 165 170 175 KQKNGIKVNFKIRHNIEDGS EGFP KQKNGIKVNFKIRHNIEDGS a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c

4320 180 185 190 195 VQLADHYQQNTPIGDGPVLL EGFP 180 185 190 195 VQLADHYQQNTPIGDGPVLL EGFP VQLADHYQQNTPIGDGPVLL t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g

4380 200 205 210 215 PDNHYLSTQSALSKDPNEKR EGFP 200 205 210 215 PDNHYLSTQSALSKDPNEKR EGFP PDNHYLSTQSALSKDPNEKR c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c

4440 220 225 230 235 DHMVLLEFVTAAGITLGMDE EGFP 220 225 230 235 DHMVLLEFVTAAGITLGMDE EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C DHMVLLEFVTAAGITLGMDE a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g

4500 LYK GSG 140 145 150 EGRGSLLTCGDVEE EGFP T2A LYK (in frame with EGFP) GSGEGRGSLLTCGDVEE EGFP LYKGSGEGRGSLLTCGDVEE t g t a c a a g g g c t c c g g c g a g g g c a g g g g a a g t c t a c t a a c a t g c g g g g a c g t g g a g g a a a a c a t g t t c c c g a g g c c g c t c c c g t c c c c t t c a g a t g a t t g t a c g c c c c t g c a c c t c c t t t

4560 (in frame with EGFP) NPGPTGMGIINTLQKYYCRV 155 NPGP (in frame with T2A) TGMGIINTLQKYYCRV T2A NPGPTGMGIINTLQKYYCRV a t c c c g g c c c a a c c g g t a t g g g a a t t a t c a a c a c c c t t c a a a a a t a c t a c t g t c g c g t c c t a g g g c c g g g t t g g c c a t a c c c t t a a t a g t t g t g g g a a g t t t t t a t g a t g a c a g c g c a g g

4620 (in frame with EGFP) RGGRCAVLSCLPKEEQIGKC (in frame with T2A) RGGRCAVLSCLPKEEQIGKC RGGRCAVLSCLPKEEQIGKC g t g g g g g t c g t t g c g c a g t c c t g t c g t g c c t g c c t a a a g a g g a g c a g a t c g g t a a a t g c t c a c c c c c a g c a a c g c g t c a g g a c a g c a c g g a c g g a t t t c t c c t c g t c t a g c c a t t t a c g a

4680 (in frame with EGFP) STRGRKCCRRKKGGSGGSGG (in frame with T2A) STRGRKCCRRKKGGSGGSGG STRGRKCCRRKKGGSGGSGG c a a c c c g t g g a c g t a a g t g c t g t c g t c g c a a g a a a g g c g g t t c t g g a g g c t c a g g g g g g a g t t g g g c a c c t g c a t t c a c g a c a g c a g c g t t c t t t c c g c c a a g a c c t c c g a g t c c c c c c t

4740 (in frame with EGFP) SGGSGGSGGSMAVPETRPNH (in frame with T2A) SGGSGGSGGSMAVPETRPNH SGGSGGSGGSMAVPETRPNH g c g g a g g g t c c g g t g g a t c a g g a g g c t c c a t g g c a g t t c c c g a g a c c c g c c c t a a c c a c a c g c c t c c c a g g c c a c c t a g t c c t c c g a g g t a c c g t c a a g g g c t c t g g g c g g g a t t g g t g t

4800 (in frame with EGFP) TIYINNLNSKIKKDELKKSL (in frame with T2A) TIYINNLNSKIKKDELKKSL TIYINNLNSKIKKDELKKSL c t a t t t a t a t c a a c a a c c t c a a t a g c a a g a t c a a g a a g g a t g a g c t a a a a a a g t c c c t g t g a t a a a t a t a g t t g t t g g a g t t a t c g t t c t a g t t c t t c c t a c t c g a t t t t t t c a g g g a c a

4860 (in frame with EGFP) YAIFSQFGQILDILVPRQRT (in frame with T2A) YAIFSQFGQILDILVPRQRT YAIFSQFGQILDILVPRQRT a c g c c a t c t t c t c c c a g t t t g g c c a g a t c c t g g a t a t c c t g g t a c c g c g g c a g c g t a c c c t g c g g t a g a a g a g g g t c a a a c c g g t c t a g g a c c t a t a g g a c c a t g g c g c c g t c g c a t g g g

4920 (in frame with EGFP) PRGQAFVIFKEVSSATNALR (in frame with T2A) PRGQAFVIFKEVSSATNALR PRGQAFVIFKEVSSATNALR c g a g g g g c c a g g c c t t t g t c a t c t t c a a g g a g g t c a g c a g c g c c a c c a a c g c c c t g c g c t g c t c c c c g g t c c g g a a a c a g t a g a a g t t c c t c c a g t c g t c g c g g t g g t t g c g g g a c g c g a

4980 (in frame with EGFP) SMQGFPFYDKPMRIQYAKTD (in frame with T2A) SMQGFPFYDKPMRIQYAKTD SMQGFPFYDKPMRIQYAKTD c c a t g c a g g g t t t c c c t t t c t a t g a c a a a c c t a t g c g t a t c c a g t a t g c c a a g a c c g a c a g g t a c g t c c c a a a g g g a a a g a t a c t g t t t g g a t a c g c a t a g g t c a t a c g g t t c t g g c t g t

5040 (in frame with EGFP) KRIPAKMKGTFVGSLQ 1 LPPL NES (in frame with T2A) KRIPAKMKGTFVGSLQLPPL KRIPAKMKGTFVGSLQLPPL a a c g t a t c c c g g c c a a g a t g a a a g g c a c c t t c g t g g g a t c c c t t c a a c t g c c t c c a c t t g t t g c a t a g g g c c g g t t c t a c t t t c c g t g g a a g c a c c c t a g g g a a g t t g a c g g a g g t g a a c

5100 (in frame with T2A) ERLTLGGSGGSGGSGGSGGS 5 ERLTL (in frame with NES) GGSGGSGGSGGSGGS NES ERLTLGGSGGSGGSGGSGGS a a a g a c t g a c a c t g g g c g g t a g t g g a g g a a g c g g g g g g a g c g g a g g g a g t g g t g g a t c c g t t t c t g a c t g t g a c c c g c c a t c a c c t c c t t c g c c c c c c t c g c c t c c c t c a c c a c c t a g g c

5160 (in frame with T2A) GGSMSASSLLEQRPKGQGNK (in frame with NES) GGSMSASSLLEQRPKGQGNK GGSMSASSLLEQRPKGQGNK g c g g c t c c a t g t c g g c c a g c a g c c t c t t g g a g c a g a g a c c a a a a g g t c a a g g a a a c a a a g c g c c g a g g t a c a g c c g g t c g t c g g a g a a c c t c g t c t c t g g t t t t c c a g t t c c t t t g t t t c

5220 (in frame with T2A) VQNGSVHQKDGLNDDDFEPY (in frame with NES) VQNGSVHQKDGLNDDDFEPY VQNGSVHQKDGLNDDDFEPY t a c a a a a t g g a t c t g t a c a t c a a a a g g a t g g a t t a a a c g a t g a t g a t t t t g a a c c t t a c t a t g t t t t a c c t a g a c a t g t a g t t t t c c t a c c t a a t t t g c t a c t a c t a a a a c t t g g a a t g a

5280 (in frame with T2A) LSPQARPNNAYTAMSDSYLP (in frame with NES) LSPQARPNNAYTAMSDSYLP LSPQARPNNAYTAMSDSYLP t g a g t c c a c a g g c a a g g c c c a a t a a t g c a t a t a c t g c c a t g t c a g a t t c c t a c t t a c c c a a c t c a g g t g t c c g t t c c g g g t t a t t a c g t a t a t g a c g g t a c a g t c t a a g g a t g a a t g g g t

5340 (in frame with T2A) SYYSPSIGFSYSLGEAAWST (in frame with NES) SYYSPSIGFSYSLGEAAWST SYYSPSIGFSYSLGEAAWST g t t a c t a c a g t c c c t c c a t t g g c t t c t c c t a t t c t t t g g g t g a a g c t g c t t g g t c t a c g g c a a t g a t g t c a g g g a g g t a a c c g a a g a g g a t a a g a a a c c c a c t t c g a c g a a c c a g a t g c c

BlpI 5400 (in frame with T2A) GGDTAMPYLTSYGQLSNGEP (in frame with NES) GGDTAMPYLTSYGQLSNGEP GGDTAMPYLTSYGQLSNGEP g g g g t g a c a c a g c c a t g c c c t a c t t a a c t t c t t a t g g a c a g c t g a g c a a c g g a g a g c c c c c c c c a c t g t g t c g g t a c g g g a t g a a t t g a a g a a t a c c t g t c g a c t c g t t g c c t c t c g g g g

5460 (in frame with T2A) HFLPDAMFGQPGALGSTPFL (in frame with NES) HFLPDAMFGQPGALGSTPFL HFLPDAMFGQPGALGSTPFL a c t t c c t a c c a g a t g c a a t g t t t g g g c a a c c a g g a g c c c t a g g t a g c a c t c c a t t t c t t g t g a a g g a t g g t c t a c g t t a c a a a c c c g t t g g t c c t c g g g a t c c a t c g t g a g g t a a a g a a c

5520 (in frame with T2A) GQHGFNFFPSGIDFSAWGNN (in frame with NES) GQHGFNFFPSGIDFSAWGNN GQHGFNFFPSGIDFSAWGNN g t c a g c a t g g t t t t a a t t t c t t t c c c a g t g g g a t t g a c t t c t c a g c a t g g g g a a a t a a c a c a g t c g t a c c a a a a t t a a a g a a a g g g t c a c c c t a a c t g a a g a g t c g t a c c c c t t t a t t g t

5580 (in frame with T2A) SSQGQSTQSSGYSSNYAYAP (in frame with NES) SSQGQSTQSSGYSSNYAYAP SSQGQSTQSSGYSSNYAYAP g t t c t c a g g g a c a g t c t a c t c a g a g c t c t g g a t a t a g t a g c a a t t a t g c t t a t g c a c c t a c a a g a g t c c c t g t c a g a t g a g t c t c g a g a c c t a t a t c a t c g t t a a t a c g a a t a c g t g g a t

5640 (in frame with T2A) SSLGGAMIDGQSAFANETLN (in frame with NES) SSLGGAMIDGQSAFANETLN SSLGGAMIDGQSAFANETLN *SKGILGEI g c t c c t t a g g t g g a g c c a t g a t t g a t g g a c a g t c a g c t t t t g c c a a t g a g a c c c t c a a t a c g a g g a a t c c a c c t c g g t a c t a a c t a c c t g t c a g t c g a a a a c g g t t a c t c t g g g a g t t a t

5700 (in frame with T2A) KAPGMNTIDQGMAAGSDYKD (in frame with NES) KAPGMNTIDQGMAAGS 1 DYKD FLAG KAPGMNTIDQGMAAGSDYKD LSRAHISYVLPHCCSGIVLV a g g c t c c t g g c a t g a a t a c t a t a g a c c a a g g g a t g g c a g c a g g a t c c g a t t a c a a g g a c g t c c g a g g a c c g t a c t t a t g a t a t c t g g t t c c c t a c c g t c g t c c t a g g c t a a t g t t c c t g c

BmtINheI 5760 (in frame with T2A) DDDK* 5 DDDK * FLAG DDDK* VIVLLSAPAEARRRCGPHSE a c g a t g a c a a g t a g g c t a g c g g g a g c c t c t g c c c t c c t c c t g c a g c c c g g a t g g c t c t c c t g c t a c t g t t c a t c c g a t c g c c c t c g g a g a c g g g a g g a g g a c g t c g g g c c t a c c g a g a g g

BstEII 5820 ETKPKSGKTQGEKPHGPHGG t c t g t t t t t g g t t t g c t t c c t t t t g t t t g c c c t t c t t t t g g g t g a c c g g g g t g g c c a c c a a g a c a a a a a c c a a a c g a a g g a a a a c a a a c g g g a a g a a a a c c c a c t g g c c c c a c c g g t g g t

5880 NRDRGLPSPLLAKQWSSSCT t t c c t g t c c c t g c c c a g t g g g g a g g g g a g c a a g g c c t t c t g c c a a g a g g a g g a g c a t g t c a a g g a c a g g g a c g g g t c a c c c c t c c c c t c g t t c c g g a a g a c g g t t c t c c t c c t c g t a c a g

5940 GQWQQMCGPGTVRHALGAAQ c c c t g c c a c t g t t g c a t g c a c c c c g g c c c t g t g a c t c t g t g t g c a a g c c c a g c a g c c t g t g g g a c g g t g a c a a c g t a c g t g g g g c c g g g a c a c t g a g a c a c a c g t t c g g g t c g t c g g a c a

6000 PAPHWSPVPRVNWSLPLRPH g g g g c a g g g t g c c a a g a g g g g a c g g g a c g g a c g t t c c a g g a c a g a g g g a g t c g a g g a t g t c c c c g t c c c a c g g t t c t c c c c t g c c c t g c c t g c a a g g t c c t g t c t c c c t c a g c t c c t a c a

6060 SGPRTPPPLTPFPLFAKSAL g a t c c a g g a c g g g t g g g g g g t g g a a g g g t g g g a a a g g g c a g g a a g g c c t t g g a a g c t a a c c t a g g t c c t g c c c a c c c c c c a c c t t c c c a c c c t t t c c c g t c c t t c c g g a a c c t t c g a t t g

6120 MSESSSPSPYLPHCEPRVSA a t g c t t t c a c t a c t g g a g g g g g a c g g a t a g a g c g g a t g g c a c t c g g g t c t c a c t g a c g c t t a c g a a a g t g a t g a c c t c c c c c t g c c t a t c t c g c c t a c c g t g a g c c c a g a g t g a c t g c g a

BstXI 6180 TRFGDPQSESTLSPLPSDDA g t t c t g a a a c c a t c t g g c t g g c t t t c t g a g g t c a g g c t g g g t a g g g g g c t g t c a t c g g c c c a a g a c t t t g g t a g a c c g a c c g a a a g a c t c c a g t c c g a c c c a t c c c c c g a c a g t a g c c g g

6240 MSSIVSARM a t a c t g c t g a t c a c a c t t g c c c t c a t c c g t c c a g c g a t c t c a c g c t g t t c t g t a a a t a c c t a t g a c g a c t a g t g t g a a c g g g a g t a g g c a g g t c g c t a g a g t g c g a c a a g a c a t t t a t g g

6300 t g g t g c t a a c a t c c c a t g c c g c t c c c c c a t g g c a c c c c c a g c c t c c t g c c c a g t t c a g g c a c c a c g a t t g t a g g g t a c g g c g a g g g g g t a c c g t g g g g g t c g g a g g a c g g g t c a a g t c c g

6360 t g g t c c a g g a a t g g a t g t g g g g g a t g g t c g c t g t g t a a c g t g c t g g t g a t c t t t t t c c c c a c c a g g t c c t t a c c t a c a c c c c c t a c c a g c g a c a c a t t g c a c g a c c a c t a g a a a a a g g g g

6420 t a c c c c t t t t a t g a c c c t t a a a a c t t t g a t t t t g t c c t a a a c a t g c c g g g c c a a c t a a a g a t g g g g a a a a t a c t g g g a a t t t t g a a a c t a a a a c a g g a t t t g t a c g g c c c g g t t g a t t t c

BsmBIEsp3I 6480 g g t g g g g a g a g g a g a g a c g a g c c c c a c c a t g c t c a c a a g a a c c a a c c t g c a a t a a a c a c t c c a c c c c t c t c c t c t c t g c t c g g g g t g g t a c g a g t g t t c t t g g t t g g a c g t t a t t t g t g a

6540 t c t g t g g g c c g c c g c g c a g a g c a c g c a g c g g g c a c c t c c t g c c c c t c c a t g g c g a c t t g g a g a c a c c c g g c g g c g c g t c t c g t g c g t c g c c c g t g g a g g a c g g g g a g g t a c c g c t g a a c c

6600 c a t c g c t t t c t g c c c g c c t c t g c c c t c g c c c t g c c t g c c c c a c c c c c c a g c g g a g a g c a t g t a g c g a a a g a c g g g c g g a g a c g g g a g c g g g a c g g a c g g g g t g g g g g g t c g c c t c t c g t a

6660 g a t c c g c g c c c t t g c t t c t g a c t c t a g c c t c t g g g a c a c a t c a g t c a a c g t g g g c a g t t c c t a g g c g c g g g a a c g a a g a c t g a g a t c g g a g a c c c t g t g t a g t c a g t t g c a c c c g t c a a g

6720 a g t c t g g g t t c g t t c c t t t c t c t g t t c t c a t c t g g c c c c c c a g g g c g a t c t t g t t g c t t t t c a g a c c c a a g c a a g g a a a g a g a c a a g a g t a g a c c g g g g g g t c c c g c t a g a a c a a c g a a a

XbaI 6780 U6 promoter G A G G G C C T A T T T C C C A T G A T T hU6-F t t g c t g g g a c c t g c c c a g t c t a g a g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t t t g c a t a a c g a c c c t g g a c g g g t c a g a t c t c t c c c g g a t a a a g g g t a c t a a g g a a g t a t a a a c g t a

6840 U6 promoter a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a c a c a a a g a t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t g t g t t t c t a t

6900 U6 promoter t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t a a a a a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a a a a t t t t

6960 U6 promoter G A C T A T C A T A T G C T T A C C G T LKO.1 5' t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t c g a t t t c t t g a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a g c t a a a g a a c

7020 U6 promoter g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g g c c a g a t c t g a g c c t g g g a g c t c t c t c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c c g g t c t a g a c t c g g a c c c t c g a g a g a

EcoRI 7080 g g c c t t a t t c c a a a t a c t a g c c t a a c c t t t t t g t t t t t t t t t t t g a a t t c g a t a t c a a g c c c g g a a t a a g g t t t a t g a t c g g a t t g g a a a a a c a a a a a a a a a a a c t t a a g c t a t a g t t c g

7140 WPRE t t a t c g a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a a t a g c t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a

7200 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R MLL MPLYHAI a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c

7260 WPRE LPVWLSFSPPCINPGCCLFM ASRMAFIFSSLYKSWLLSLY c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c

7320 WPRE RSCGPLSGNVAWCALCLLTQ EELWPVVRQRGVVCTVFADA a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t

7380 WPRE PPLVGALPPPVSSFPGLSLS TPTGWGIATTCQLLSGTFAF c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g

7440 WPRE PSLLPRRNSSPPALPAAGQG PLPIATAELIAACLARCWTG c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c

7500 WPRE *RGKR LGCWALTIPWCCRGNHRPFL ARLLGTDNSVVLSGKSSSFP c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a

7560 WPRE (in frame with Factor Xa site) PQEGTNGGPNQAPRGEAVDR Factor Xa site GCSPVLPPGFCAGRPSATSL WLLACVATWILRGTSFCYVP g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a

7620 WPRE 1 RGEI Factor Xa site RPSIQRTFLPAACCRLCGLF SALNPADLPSRGLLPALRPL c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g

7680 WPRE KS primer RVFAFALRRVGSPFGPPPRI PRLRLRPQTSRISLWAASPH c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g

PaeR7IXhoI 7740 KS primer C T A T G G C A G C T G G A G C T pBluescriptKS DTVDLET* RYRRPRDLEKHGAITSSNTA g a t a c c g t c g a c c t c g a g a c c t a g a a a a a c a t g g a g c a a t c a c a a g t a g c a a t a c a g c a g c t a t g g c a g c t g g a g c t c t g g a t c t t t t t g t a c c t c g t t a g t g t t c a t c g t t a t g t c g t c

7800 ATNADCAWLEAQEEEEVGFP c t a c c a a t g c t g a t t g t g c c t g g c t a g a a g c a c a a g a g g a g g a g g a g g t g g g t t t t c c a g g a t g g t t a c g a c t a a c a c g g a c c g a t c t t c g t g t t c t c c t c c t c c t c c a c c c a a a a g g t c

7860 VTPQVPLRPMTYKAAVDLSH t c a c a c c t c a g g t a c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t a g t g t g g a g t c c a t g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a

7920 FLKEKGGLEGLIHSQRRQDI t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t a t c c a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a t a g g

7980 LDLWIYHTQGYFPDWQNYTP t t g a t c t g t g g a t c t a c c a c a c a c a a g g c t a c t t c c c t g a t t g g c a g a a c t a c a c a c c a g a a c t a g a c a c c t a g a t g g t g t g t g t t c c g a t g a a g g g a c t a a c c g t c t t g a t g t g t g g t c

8040 GPGIRYPLTFGWCYKLVPVE g g c c a g g g a t c a g a t a t c c a c t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c c c g g t c c c t a g t c t a t a g g t g a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g

8100 QEKVEEANEGENTRLLHPVS a a g a g a a g g t a g a a g a a g c c a a t g a a g g a g a g a a c a c c c g c t t g t t a c a c c c t g t g a g c c t t c t c t t c c a t c t t c t t c g g t t a c t t c c t c t c t t g t g g g c g a a c a a t g t g g g a c a c t c g g

8160 LHGMDDPEREVLEWRFDSRL t g c a t g g g a t g g a t g a c c c g g a g a g a g a a g t a t t a g a g t g g a g g t t t g a c a g c c g c c t a g a c g t a c c c t a c c t a c t g g g c c t c t c t c t t c a t a a t c t c a c c t c c a a a c t g t c g g c g g a t c

BspEI 8220 5' LTR (truncated) AFHHMARELHPDCTGSLWLD c a t t t c a t c a c a t g g c c c g a g a g c t g c a t c c g g a c t g t a c t g g g t c t c t c t g g t t a g a c c g t a a a g t a g t g t a c c g g g c t c t c g a c g t a g g c c t g a c a t g a c c c a g a g a g a c c a a t c t g g

8280 5' LTR (truncated) QI* a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t

8340 5' LTR (truncated) g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t

PmeIPspOMIApaI 8400 5' LTR (truncated) g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g g g c c c g t t t a a a c c c g c c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c c c g g g c a a a t t t g g g c g

8460 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c

8520 bGH poly(A) signal c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a

8580 bGH poly(A) signal g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g

8640 bGH poly(A) signal a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a

8700 f1 ori t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g

8760 f1 ori g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g

8820 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g

8880 f1 ori c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g

8940 f1 ori g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c

9000 f1 ori G T G G A C T C T T G T T C C A A A C T F1ori-F g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a

9060 f1 ori G G F1ori-F g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a

9120 f1 ori t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a

9180 SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g

9240 SV40 promoter a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c

9300 SV40 promoter g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g

9360 SV40 promoter SV40 ori c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t

9420 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a

9480 SV40 promoter SV40 ori g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t

9540 EM7 promoter t t t t c g g a t c t g a t c a g c a c g t g t t g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a a a a g c c t a g a c t a g t c g t g c a c a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a

9600 1 5 10 MAKLTSAVPVL EM7 promoter BleoR a t a a t a c g a c a a g g t g a g g a a c t a a a c c a t g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t t a t t a t g c t g t t c c a c t c c t t g a t t t g g t a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a

MauBI 9660 15 20 25 30 TARDVAGAVEFWTDRLGFSR BleoR c a c c g c g c g c g a c g t c g c c g g a g c g g t c g a g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g t g g c g c g c g c t g c a g c g g c c t c g c c a g c t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c

SgrAI 9720 35 40 45 50 DFVEDDFAGVVRDDVTLFIS BleoR g g a c t t c g t g g a g g a c g a c t t c g c c g g t g t g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c c t g a a g c a c c t c c t g c t g a a g c g g c c a c a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c

9780 55 60 65 70 AVQDQVVPDNTLAWVWVRGL BleoR c g c g g t c c a g g a c c a g g t g g t g c c g g a c a a c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g c g c c a g g t c c t g g t c c a c c a c g g c c t g t t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a

9840 75 80 85 90 DELYAEWSEVVSTNFRDASG BleoR g g a c g a g c t g t a c g c c g a g t g g t c g g a g g t c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g c c t g c t c g a c a t g c g g c t c a c c a g c c t c c a g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c

FseI 9900 95 100 105 110 PAMTEIGEQPWGREFALRDP BleoR g c c g g c c a t g a c c g a g a t c g g c g a g c a g c c g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c c g g c c g g t a c t g g c t c t a g c c g c t c g t c g g c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g

9960 115 120 125 AGNCVHFVAEEQD* BleoR g g c c g g c a a c t g c g t g c a c t t c g t g g c c g a g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t c c g g c c g t t g a c g c a c g t g a a g c a c c g g c t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a

10,020 c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c

10,080 SV40 poly(A) signal c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a

10,140 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g

10,200 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a

BstZ17I 10,260 M13 rev C A G T A T C G A C A A A G G A C M13 Reverse G G A C A M13/pUC Reverse c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a

10,320 -10 -35 lac operator lac promoter C A C T T T A A C A A T A G G C G A M13/pUC Reverse g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t

10,380 lac promoter CAP binding site a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g

10,440 t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c

10,500 G A G C G A G T G A C T G A G C G A L4440 a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a

10,560 c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t

PciI 10,620 a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c

10,680 ori t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t

10,740 ori T T T C T A T G G T C C G C A A pBR322ori-F a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a

10,800 ori A G G G pBR322ori-F t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a

10,860 ori g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a

10,920 ori c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g

10,980 ori c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a

11,040 ori a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g

11,100 ori t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a

11,160 ori c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t

11,220 ori a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t

11,280 ori a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t

11,340 a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a

11,400 t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t

11,460 285 280 275 270 *WHKILSAGIEAIQRNRED AmpR *WHKILSAGIEAIQRNRED c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g

AhdI 11,520 265 260 255 250 MTAQSGTTYIVVIRSPKGDP AmpR MTAQSGTTYIVVIRSPKGDP c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c

11,580 245 240 235 230 GLAAIIGRSGREGAGSKDAI AmpR GLAAIIGRSGREGAGSKDAI c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a

11,640 225 220 215 210 FWGAPLASRLLPGAVKDAEM AmpR FWGAPLASRLLPGAVKDAEM a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a

11,700 205 200 195 190 WDILQQRSALTLLEGTLLKR AmpR WDILQQRSALTLLEGTLLKR c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c

11,760 185 180 175 170 LTTAMAVPMTTDREDNPIAE AmpR LTTAMAVPMTTDREDNPIAE c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g

11,820 165 160 155 150 NLEPEWRDLRTVHDGMNHLF AmpR NLEPEWRDLRTVHDGMNHLF a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t

PvuI 11,880 145 140 135 130 ATLEKPGGITTLLLNAATND AmpR ATLEKPGGITTLLLNAATND a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g

11,940 125 120 115 110 SMTIAASCLERVTMGDTLHK AmpR SMTIAASCLERVTMGDTLHK a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a

ScaI 12,000 105 100 95 90 ETVPSYEVLDNQSYHIRRGL AmpR ETVPSYEVLDNQSYHIRRGL t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c

12,060 85 80 75 70 QEQGADIRSLVAGCLLVKFT AmpR A T A A T A C C G C G C C A C A T A G C Amp-R QEQGADIRSLVAGCLLVKFT t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a

12,120 65 60 55 50 SMMPFREEPRFSELIKGSNL AmpR SMMPFREEPRFSELIKGSNL g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c

12,180 45 40 35 30 DLEIYGVRAGLQDEADKVKV AmpR DLEIYGVRAGLQDEADKVKV a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g

12,240 25 LTEPH 20 15 10 signal sequence AFVPLCFAAFFPILA AmpR LTEPHAFVPLCFAAFFPILA c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g

SspI 12,300 5 1 signal sequence VRFHQISM AmpR AmpR promoter VRFHQISM g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t

12,360 AmpR promoter g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c

3ʹ 5ʹ 12,397 AmpR promoter g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.