Addgene pCMV-FLAG-p31(comet)-QF Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pCMV-FLAG-p31(comet)-QF Sequencing Result


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NdeI (4980) SnaBI (4876) CMV-F (4678 .. 4698) BmtI (4625) NheI (4621) SacI (4571) Eco53kI (4569) SacII (4562) NotI (4550) SrfI (4512) BamHI (4503) PstI (4495) EcoRI (4485) AflII (4200) XmnI (4184) SpeI (4045) BsmBI - Esp3I (3909) PflMI (3720) BbsI (3701) HindIII (3654) AccI (3640) SalI (3639) pBluescriptKS (3634 .. 3650) AbsI - PaeR7I - PspXI - XhoI (3633) ApaI (3628) PspOMI (3624) T7 (3560 .. 3579) PvuI (3548) BclI * (3388) MfeI (3295) HpaI (3286) SV40pA-R (3230 .. 3249) BtsI - BtsαI (3212) EBV-rev (3176 .. 3195) MluI (3159) F1ori-F (3000 .. 3021) DraIII (2936) F1ori-R (2790 .. 2809) CMV enhancer PciI (127) pBR322ori-F (268 .. 287) ApaLI (441) BsaI (1056) TK-pA-R (1177 .. 1196) PfoI (1269) BstBI (1365) Neo-F (1495 .. 1514) RsrII (1530) MscI (1967) Neo-R (2105 .. 2124) SfiI (2277) SV40 promoter pBABE 3' (2527 .. 2547) pCMV-FLAG-p31(comet)-QF 5143 bp

Sequence View

5ʹ 3ʹ 60 CMV enhancer a a g t t a t g t a a c g c g g a a c t c c a t a t a t g g g c t a t g a a c t a a t g a c c c c g t a a t t g a t t a t t c a a t a c a t t g c g c c t t g a g g t a t a t a c c c g a t a c t t g a t t a c t g g g g c a t t a a c t a a t

120 c t a t t a a t a a c t a a t g c a t g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a t a a t t a t t g a t t a c g t a c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c

PciI 180 g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g

240 ori t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g

300 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g

360 ori t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a

420 ori c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c

ApaLI 480 ori t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a

540 ori c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c

600 ori c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a

660 ori g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g

720 ori c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t

780 ori g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c

840 a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t

900 t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t

960 *LIYSYGSAIAPGAAG g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a c c t g a g g c t a t g g c a g g g c c t g c c g c c c c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t g g a c t c c g a t a c c g t c c c g g a c g g c g g g

1020 VNAALGPGEGSSPPHPPSFS c g a c g t t g g c t g c g a g c c c t g g g c c t t c a c c c g a a c t t g g g g g g t g g g g t g g g g a a a a g g g c t g c a a c c g a c g c t c g g g a c c c g g a a g t g g g c t t g a a c c c c c c a c c c c a c c c c t t t t c c

BsaI 1080 SVRAYQGWHPRPPTDVSHWG a a g a a a c g c g g g c g t a t t g g c c c c a a t g g g g t c t c g g t g g g g t a t c g a c a g a g t g c c a g c t t c t t t g c g c c c g c a t a a c c g g g g t t a c c c c a g a g c c a c c c c a t a g c t g t c t c a c g g t c g

1140 HSV TK poly(A) signal QSRVGRKHVFSGVGHTKNQR c c t g g g a c c g a a c c c c g c g t t t a t g a a c a a a c g a c c c a a c a c c g t g c g t t t t a t t c t g t c g g a c c c t g g c t t g g g g c g c a a a t a c t t g t t t g c t g g g t t g t g g c a c g c a a a a t a a g a c a g

1200 HSV TK poly(A) signal T T G T C T C C T T C C G T G T T T C A TK-pA-R KIATMARTGEPITEKRTETL t t t t t a t t g c c g t c a t a g c g c g g g t t c c t t c c g g t a t t g t c t c c t t c c g t g t t t c a g t t a a a a a a t a a c g g c a g t a t c g c g c c c a a g g a a g g c c a t a a c a g a g g a a g g c a c a a a g t c a a t

1260 RGRPHAFFELMLDGRQLIMW g c c t c c c c c t a g g g t g g g c g a a g a a c t c c a g c a t g a g a t c c c c g c g c t g g a g g a t c a t c c c g g a g g g g g a t c c c a c c c g c t t c t t g a g g t c g t a c t c t a g g g g c g c g a c c t c c t a g t a g g

PfoI 1320 GADRFVIGFGLREYFAATSD a g c c g g c g t c c c g g a a a a c g a t t c c g a a g c c c a a c c t t t c a t a g a a g g c g g c g g t g g a a t t c g g c c g c a g g g c c t t t t g c t a a g g c t t c g g g t t g g a a a g t a t c t t c c g c c g c c a c c t t a

BstBI 1380 MAGWASLGRSFRTPES FDRSPLNPTAQDTM c g a a a t c t c g t g a t g g c a g g t t g g g c g t c g c t t g g t c g g t c a t t t c g a a c c c c a g a g t c c g c t t t a g a g c a c t a c c g t c c a a c c c g c a g c g a a c c a g c c a g t a a a g c t t g g g g t c t c a g g

1440 265 260 255 250 *FFEDLLRYFAIRQSDPAA NeoR/KanR 265 260 255 250 *FFEDLLRYFAIRQSDPAA NeoR/KanR RSEELVKKAIEGDALRIGSG *FFEDLLRYFAIRQSDPAA c g c t c a g a a g a a c t c g t c a a g a a g g c g a t a g a a g g c g a t g c g c t g c g a a t c g g g a g c g g c g c g a g t c t t c t t g a g c a g t t c t t c c g c t a t c t t c c g c t a c g c g a c g c t t a g c c c t c g c c g

1500 245 240 235 230 IGYLVLFRDAWEGGLEEAID NeoR/KanR 245 240 235 230 IGYLVLFRDAWEGGLEEAID NeoR/KanR T T A T A G Neo-F DTVKHEEAVSPFAAKFFSNI IGYLVLFRDAWEGGLEEAID g a t a c c g t a a a g c a c g a g g a a g c g g t c a g c c c a t t c g c c g c c a a g t t c t t c a g c a a t a t c c t a t g g c a t t t c g t g c t c c t t c g c c a g t c g g g t a a g c g g c g g t t c a a g a a g t c g t t a t a g

RsrII 1560 225 220 215 210 RTALAIDQYRDAVGLRGCDI NeoR/KanR 225 220 215 210 RTALAIDQYRDAVGLRGCDI NeoR/KanR T G C C C A T C G G T T G C Neo-F TGSQRYVLIAVRHTQPATVD RTALAIDQYRDAVGLRGCDI a c g g g t a g c c a a c g c t a t g t c c t g a t a g c g g t c c g c c a c a c c c a g c c g g c c a c a g t c g a t t g c c c a t c g g t t g c g a t a c a g g a c t a t c g c c a g g c g g t g t g g g t c g g c c g g t g t c a g c t a

1620 205 200 195 190 FGSFRGNEVMINPLCADGHT NeoR/KanR 205 200 195 190 FGSFRGNEVMINPLCADGHT NeoR/KanR ESRKAAIFHHDIRQAGIAMG FGSFRGNEVMINPLCADGHT g a a t c c a g a a a a g c g g c c a t t t t c c a c c a t g a t a t t c g g c a a g c a g g c a t c g c c a t g g g t c t t a g g t c t t t t c g c c g g t a a a a g g t g g t a c t a t a a g c c g t t c g t c c g t a g c g g t a c c c a

1680 185 180 175 170 VVLDEGDPMSAKLRAFLEAP NeoR/KanR 185 180 175 170 VVLDEGDPMSAKLRAFLEAP NeoR/KanR HDEILAVGHARLEPGEQFGW VVLDEGDPMSAKLRAFLEAP c a c g a c g a g a t c c t c g c c g t c g g g c a t g c t c g c c t t g a g c c t g g c g a a c a g t t c g g c t g g g t g c t g c t c t a g g a g c g g c a g c c c g t a c g a g c g g a a c t c g g a c c g c t t g t c a a g c c g a c c

1740 165 160 155 150 ALGQHEEDLDDQDVLGAEMR NeoR/KanR 165 160 155 150 ALGQHEEDLDDQDVLGAEMR NeoR/KanR REPLMFFVQIILIDKTGFHP ALGQHEEDLDDQDVLGAEMR c g c g a g c c c c t g a t g t t c t t c g t c c a g a t c a t c c t g a t c g a c a a g a c c g g c t t c c a t c c g g c g c t c g g g g a c t a c a a g a a g c a g g t c t a g t a g g a c t a g c t g t t c t g g c c g a a g g t a g g c

1800 145 140 135 130 TRAREIRHKAQHDFPCTAPD NeoR/KanR 145 140 135 130 TRAREIRHKAQHDFPCTAPD NeoR/KanR STCSLDAMFRLVVEWAGSRI TRAREIRHKAQHDFPCTAPD a g t a c g t g c t c g c t c g a t g c g a t g t t t c g c t t g g t g g t c g a a t g g g c a g g t a g c c g g a t c t c a t g c a c g a g c g a g c t a c g c t a c a a a g c g a a c c a c c a g c t t a c c c g t c c a t c g g c c t a g

1860 125 120 115 110 LTHLRRMADAMISVKEAPAL NeoR/KanR 125 120 115 110 LTHLRRMADAMISVKEAPAL NeoR/KanR KRMQPPHCISHDGYFLGRSK LTHLRRMADAMISVKEAPAL a a g c g t a t g c a g c c g c c g c a t t g c a t c a g c c a t g a t g g a t a c t t t c t c g g c a g g a g c a a g t t c g c a t a c g t c g g c g g c g t a a c g t a g t c g g t a c t a c c t a t g a a a g a g c c g t c c t c g t t c

1920 105 100 95 90 HSSLLDQGPVEGLLLWDRGA NeoR/KanR 105 100 95 90 HSSLLDQGPVEGLLLWDRGA NeoR/KanR VR* HSSLLDQGPVEGLLLWDRGA g t g a g a t g a c a g g a g a t c c t g c c c c g g c a c t t c g c c c a a t a g c a g c c a g t c c c t t c c c g c c a c t c t a c t g t c c t c t a g g a c g g g g c c g t g a a g c g g g t t a t c g t c g g t c a g g g a a g g g c g

MscI 1980 85 80 75 70 ETVVDLVAACPVGTTALWSL NeoR/KanR 85 80 75 70 ETVVDLVAACPVGTTALWSL NeoR/KanR ETVVDLVAACPVGTTALWSL t t c a g t g a c a a c g t c g a g c a c a g c t g c g c a a g g a a c g c c c g t c g t g g c c a g c c a c g a t a g a a g t c a c t g t t g c a g c t c g t g t c g a c g c g t t c c t t g c g g g c a g c a c c g g t c g g t g c t a t c

2040 65 60 55 50 RAAEDQLENLAGSLDTKVFL NeoR/KanR 65 60 55 50 RAAEDQLENLAGSLDTKVFL NeoR/KanR RAAEDQLENLAGSLDTKVFL c c g c g c t g c c t c g t c t t g c a g t t c a t t c a g g g c a c c g g a c a g g t c g g t c t t g a c a a a a a g g g c g c g a c g g a g c a g a a c g t c a a g t a a g t c c c g t g g c c t g t c c a g c c a g a a c t g t t t t t c

2100 45 40 35 30 VPRGQASLRFVAADSCGITQ NeoR/KanR 45 40 35 30 VPRGQASLRFVAADSCGITQ NeoR/KanR VPRGQASLRFVAADSCGITQ a a c c g g g c g c c c c t g c g c t g a c a g c c g g a a c a c g g c g g c a t c a g a g c a g c c g a t t g t c t g t t g g c c c g c g g g g a c g c g a c t g t c g g c c t t g t g c c g c c g t a g t c t c g t c g g c t a a c a g a c

2160 25 20 15 10 QAWDYGFLREVWAAPSGAHL NeoR/KanR 25 20 15 10 QAWDYGFLREVWAAPSGAHL NeoR/KanR G C C C A G T C A T A G C C G A A T A G Neo-R QAWDYGFLREVWAAPSGAHL t t g t g c c c a g t c a t a g c c g a a t a g c c t c t c c a c c c a a g c g g c c g g a g a a c c t g c g t g c a a a a c a c g g g t c a g t a t c g g c t t a t c g g a g a g g t g g g t t c g c c g g c c t c t t g g a c g c a c g t t

2220 5 1 GDQEIM NeoR/KanR SV40 promoter 5 1 GDQEIM NeoR/KanR GDQEIM t c c a t c t t g t t c a a t c a t g c g a a a c g a t c c t c a t c c t g t c t c t t g a t c g a t c t t t g c a a a a g g t a g a a c a a g t t a g t a c g c t t t g c t a g g a g t a g g a c a g a g a a c t a g c t a g a a a c g t t t

SfiI 2280 SV40 promoter SV40 ori a g c c t a g g c c t c c a a a a a a g c c t c c t c a c t a c t t c t g g a a t a g c t c a g a g g c c g a g g c g g t c g g a t c c g g a g g t t t t t t c g g a g g a g t g a t g a a g a c c t t a t c g a g t c t c c g g c t c c g c c

2340 SV40 promoter SV40 ori c c t c g g c c t c t g c a t a a a t a a a a a a a a t t a g t c a g c c a t g g g g c g g a g a a t g g g c g g a a c g g a g c c g g a g a c g t a t t t a t t t t t t t t a a t c a g t c g g t a c c c c g c c t c t t a c c c g c c t t g

2400 SV40 promoter SV40 ori SV40 enhancer t g g g c g g a g t t a g g g g c g g g a t g g g c g g a g t t a g g g g c g g g a c t a t g g t t g c t g a c t a a t a c c c g c c t c a a t c c c c g c c c t a c c c g c c t c a a t c c c c g c c c t g a t a c c a a c g a c t g a t t a

2460 SV40 promoter SV40 enhancer t g a g a t g c a t g c t t t g c a t a c t t c t g c c t g c t g g g g a g c c t g g g g a c t t t c c a c a c c t g g a c t c t a c g t a c g a a a c g t a t g a a g a c g g a c g a c c c c t c g g a c c c c t g a a a g g t g t g g a c c

2520 SV40 promoter SV40 enhancer t t g c t g a c t a a t t g a g a t g c a t g c t t t g c a t a c t t c t g c c t g c t g g g g a g c c t g g g g a c t a a c g a c t g a t t a a c t c t a c g t a c g a a a c g t a t g a a g a c g g a c g a c c c c t c g g a c c c c t g a

2580 SV40 promoter AmpR promoter SV40 enhancer A C C C T A A C T G A C A C A C A T T C C pBABE 3' t t c c a c a c c c t a a c t g a c a c a c a t t c c a c a g c t g g t t c t t t c c g c c t c a g g a t t c t t c c t a a g g t g t g g g a t t g a c t g t g t g t a a g g t g t c g a c c a a g a a a g g c g g a g t c c t a a g a a g g a

2640 AmpR promoter t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t

2700 AmpR promoter a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g

2760 f1 ori t g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c a c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g

2820 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g

2880 f1 ori c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a

DraIII 2940 f1 ori t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c

3000 f1 ori G F1ori-F g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c

3060 f1 ori T G G A C T C T T G T T C C A A A C T G G F1ori-F t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a

3120 f1 ori a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a

MluI 3180 f1 ori SV40 poly(A) signal C T A C T EBV-rev t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a c g c g t t a a g a t a c a t t g a t g a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t g c g c a a t t c t a t g t a a c t a c t

BtsαIBtsI 3240 SV40 poly(A) signal G A A A T T T G T G A SV40pA-R C A A A C C T G T T T G G T G EBV-rev g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t

MfeIHpaI 3300 SV40 poly(A) signal T G C T A T T G C SV40pA-R t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c

3360 c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t a a a g c a a g t a a a a g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a a t t t c g t t c a t t t t

BclI* 3420 c c t c t a c a a a t g t g g t a t g g c t g a t t a t g a t c a t g a a c a g a c t g t g a g g a c t g a g g g g c c g g a g a t g t t t a c a c c a t a c c g a c t a a t a c t a g t a c t t g t c t g a c a c t c c t g a c t c c c c g g

3480 t g a a a t g a g c c t t g g g a c t g t g a a t c t a a a a t a c a c a a a c a a t t a g a a t c a g t a g t t t a a a c t t t a c t c g g a a c c c t g a c a c t t a g a t t t t a t g t g t t t g t t a a t c t t a g t c a t c a a a t t

3540 c a c a t t a t a c a c t t a a a a a t t g g a t c t c c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a g t g t a a t a t g t g a a t t t t t a a c c t a g a g g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t

PvuI 3600 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 a g g g c g a t c g a g t g a a t t g t a a t a c g a c t c a c t a t a g g g c g a a t t g g g t a c a c t t a c c t g t c c c g c t a g c t c a c t t a a c a t t a t g c t g a g t g a t a t c c c g c t t a a c c c a t g t g a a t g g a c

HindIIIPspOMIApaIPaeR7IPspXIXhoIAbsISalIAccI 3660 KS primer T C G A G G T C G A C G G T A T C pBluescriptKS g t a c c t t a a t t a a t t a a g g t a c c g g g c c c c c c c t c g a g g t c g a c g g t a t c g a t a a g c t t t c a t g g a a t t a a t t a a t t c c a t g g c c c g g g g g g g a g c t c c a g c t g c c a t a g c t a t t c g a a a

PflMIBbsI 3720 (in frame with FLAG) *ERFGKFTVPAQFWIYDEWA *ERFGKFTVPAQFWIYDEWA c a c t c g c g g a a g c c t t t a a a t g t c a c t g g t g c c t g g a a c c a a a t g t a g t c t t c c c a a g c c g t g a g c g c c t t c g g a a a t t t a c a g t g a c c a c g g a c c t t g g t t t a c a t c a g a a g g g t t c g g

3780 (in frame with FLAG) TTRISPRGCSLTVTLKHGRS TTRISPRGCSLTVTLKHGRS g t g g t t c g g a t g g a a g g t c t g c c a c a t g a c a g g g t c a c a g t c a g t t t a t g g c c c c g g c t g c a c c a a g c c t a c c t t c c a g a c g g t g t a c t g t c c c a g t g t c a g t c a a a t a c c g g g g c c g a c

3840 (in frame with FLAG) PVRYNLKPRFWDEGCNRHGQ PVRYNLKPRFWDEGCNRHGQ g g c a c t c g a t a g t t g a g c t t g g g t c g a a a c c a a t c t t c t c c a c a g t t g c g g t g t c c c t g t c c g t g a g c t a t c a a c t c g a a c c c a g c t t t g g t t a g a a g a g g t g t c a a c g c c a c a g g g a c a

3900 (in frame with FLAG) AMVVTGMLPPAQLESFADAM AMVVTGMLPPAQLESFADAM g c c a t g a c g a c g g t g c c c a t g a g t g g a g g a g c c t g a a g c t c g c t a a a g g c a t c a g c c a t g c g g t a c t g c t g c c a c g g g t a c t c a c c t c c t c g g a c t t c g a g c g a t t t c c g t a g t c g g t a c

BsmBIEsp3I 3960 (in frame with FLAG) FIARALRRLCAATSLSQDVS FIARALRRLCAATSLSQDVS a a t a t g g c t c g g g c g a g a c g g c g c a a a c a a g c t g c t g t g c t c a g g c t c t g g t c c a c g c t g t t a t a c c g a g c c c g c t c t g c c g c g t t t g t t c g a c g a c a c g a g t c c g a g a c c a g g t g c g a c

4020 (in frame with FLAG) YPALLSLDLEYFEKPSLANG YPALLSLDLEYFEKPSLANG t a g g g g g c c a g c a g a g a c a a g t c g a g t t c a t a g a a c t c c t t g g g g c t t a g g g c a t t g c c c a t c c c c c g g t c g t c t c t g t t c a g c t c a a g t a t c t t g a g g a a c c c c g a a t c c c g t a a c g g g

SpeI 4080 (in frame with FLAG) GLLILVRPVLTRAFFDELHS GLLILVRPVLTRAFFDELHS c c a a g g a g a a t c a g c a c t c g c g g t a c t a g t g t c c g t g c a a a g a a g t c c t c c a g g t g g c t g g g t t c c t c t t a g t c g t g a g c g c c a t g a t c a c a g g c a c g t t t c t t c a g g a g g t c c a c c g a c

4140 (in frame with FLAG) LVSELEALAQQCKRSSVETT LVSELEALAQQCKRSSVETT a g g a c a c t c t c c a g t t c t g c c a g g g c t t g t t g g c a t t t c c t g c t g c t c a c c t c a g t g g t g t c c t g t g a g a g g t c a a g a c g g t c c c g a a c a a c c g t a a a g g a c g a c g a g t g g a g t c a c c a c

XmnI 4200 (in frame with FLAG) ARPKKKLMEEAQPSPKRYFH ARPKKKLMEEAQPSPKRYFH g c c c g a g g t t t c t t c t t c a g c a t c t c c t c t g c c t g g g g a g a a g g t t t t c g g t a a a a g t g c c g g g c t c c a a a g a a g a a g t c g t a g a g g a g a c g g a c c c c t c t t c c a a a a g c c a t t t t c a c g

AflII 4260 (in frame with FLAG) KLQEYPLPLQQRAYMIHKLL KLQEYPLPLQQRAYMIHKLL t t a a g c t g t t c a t a g g g c a g a g g g a g c t g c t g g c g t g c a t a c a t g a t a t g c t t t a g a a g t a a t t c g a c a a g t a t c c c g t c t c c c t c g a c g a c c g c a c g t a t g t a c t a t a c g a a a t c t t c a

4320 (in frame with FLAG) ECTFQCCGEQSVPGPFVVPV ECTFQCCGEQSVPGPFVVPV t c a c a a g t a a a c t g a c a g c a g c c t t c c t g g c t c a c a g g c c c a g g a a a c a c c a c t g g t a c c a g t g t t c a t t t g a c t g t c g t c g g a a g g a c c g a g t g t c c g g g t c c t t t g t g g t g a c c a t g g

4380 (in frame with FLAG) MCDRPCFAESANLPEQTSST MCDRPCFAESANLPEQTSST a t g c a g t c t c t t g g g c a a a a g g c c t c c g a a g c g t t g a g a g g t t c c t g c g t a g a g c t t g t c t a c g t c a g a g a a c c c g t t t t c c g g a g g c t t c g c a a c t c t c c a a g g a c g c a t c t c g a a c a g

4440 (in frame with FLAG) ELLEIQSAHTEESKEYWELD ELLEIQSAHTEESKEYWELD t c a a g t a g t t c t a t c t g g g a g g c g t g a g t t t c t t c g g a c t t c t c a t a c c a c t c c a a a t c a a g t t c a t c a a g a t a g a c c c t c c g c a c t c a a a g a a g c c t g a a g a g t a t g g t g a g g t t t a g t

PstIEcoRI 4500 (in frame with FLAG) PVAASSLVEAEPAAMFEQLG PVAASSLVEAEPAAMFEQLG g g g a c t g c g g c t g a g g a c a g a a c c t c c g c c t c c g g c g c c g c c a t g a a t t c c t g c a g c c c g c c c t g a c g c c g a c t c c t g t c t t g g a g g c g g a g g c c g c g g c g g t a c t t a a g g a c g t c g g g c

NotISrfIBamHI 4560 (in frame with FLAG) PSGGPS 5 1 KDDDDKYD FLAG Kozak sequence PSGGPSKDDDDKYDM g g g g a t c c g c c c g g g c t c t t a t c g t c g t c a t c c t t g t a a t c c a t g g t g g c g g c c g c c a c c c c c c t a g g c g g g c c c g a g a a t a g c a g c a g t a g g a a c a t t a g g t a c c a c c g c c g g c g g t g g

SacIIEco53kISacI 4620 T3 promoter g c g g t g g a g c t c c a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t t c g a g c t t g g c g t a a t c c g c c a c c t c g a g g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a a g c t c g a a c c g c a t t a g

BmtINheI 4680 CMV promoter G T G CMV-F g c t a g c g g a t c t g a c g g t t c a c t a a a c c a g c t c t g c t t a t a t a g a c c t c c c a c c g t a c a c c g a t c g c c t a g a c t g c c a a g t g a t t t g g t c g a g a c g a a t a t a t c t g g a g g g t g g c a t g t g

4740 CMV promoter C G G A T G G C G G G T A A A C G C CMV-F g c c t a c c g c c c a t t t g c g t c a a t g g g g c g g a g t t g t t a c g a c a t t t t g g a a a g t c c c g t t c g g a t g g c g g g t a a a c g c a g t t a c c c c g c c t c a a c a a t g c t g t a a a a c c t t t c a g g g c a a

4800 CMV promoter g a t t t t g g t g c c a a a a c a a a c t c c c a t t g a c g t c a a t g g g g t g g a g a c t t g g a a a t c c c c c t a a a a c c a c g g t t t t g t t t g a g g g t a a c t g c a g t t a c c c c a c c t c t g a a c c t t t a g g g g

4860 CMV promoter CMV enhancer g t g a g t c a a a c c g c t a t c c a c g c c c a t t g a t g t a c t g c c a a a a c c g c a t c a c c a t g g t a a c a c t c a g t t t g g c g a t a g g t g c g g g t a a c t a c a t g a c g g t t t t g g c g t a g t g g t a c c a t t

SnaBI 4920 CMV enhancer t a g c g a t g a c t a a t a c g t a g a t g t a c t g c c a a g t a g g a a a g t c c c a t a a g g t c a t g t a c t a t c g c t a c t g a t t a t g c a t c t a c a t g a c g g t t c a t c c t t t c a g g g t a t t c c a g t a c a t g a

4980 CMV enhancer g g g c a t a a t g c c a g g c g g g c c a t t t a c c g t c a t t g a c g t c a a t a g g g g g c g t a c t t g g c a c c c g t a t t a c g g t c c g c c c g g t a a a t g g c a g t a a c t g c a g t t a t c c c c c g c a t g a a c c g t

NdeI 5040 CMV enhancer t a t g a t a c a c t t g a t g t a c t g c c a a g t g g g c a g t t t a c c g t a a a t a c t c c a c c c a t t g a c a t a c t a t g t g a a c t a c a t g a c g g t t c a c c c g t c a a a t g g c a t t t a t g a g g t g g g t a a c t g

5100 CMV enhancer g t c a a t g g a a a g t c c c t a t t g g c g t t a c t a t g g g a a c a t a c g t c a t t a t t g a c g t c a a t g c a g t t a c c t t t c a g g g a t a a c c g c a a t g a t a c c c t t g t a t g c a g t a a t a a c t g c a g t t a c

3ʹ 5ʹ 5143 CMV enhancer g g c g g g g g t c g t t g g g c g g t c a g c c a g g c g g g c c a t t t a c c g t c c g c c c c c a g c a a c c c g c c a g t c g g t c c g c c c g g t a a a t g g c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.