Addgene Lox-Stop-Lox TOPO Hygro - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: Lox-Stop-Lox TOPO Hygro


Map View

CAP binding site L4440 (9616 .. 9633) pBR322ori-F (9363 .. 9382) FseI (8638) BleoR CsiI - SexAI * (8525) SgrAI (8476) MauBI (8398) SfiI (8362) Neo-F (8025 .. 8044) Neo-R (7415 .. 7434) BclI * (7330) XcmI (7305) BsaI (6928) ccdB-fwd (6891 .. 6910) BsrGI (6763) BsiWI - SnaBI (6703) M13/pUC Forward (6565 .. 6587) M13 Forward (6556 .. 6573) M13 fwd T7 (6530 .. 6549) T7 promoter PspXI (6500) NotI (6494) XmnI (6040) SacII (5716) NdeI (5386) AsiSI (5300) PshAI (4963) mPGK-F (4825 .. 4844) M13/pUC Reverse (186 .. 208) lac operator M13 Reverse (205 .. 221) M13 rev SP6 (239 .. 256) SP6 promoter Acc65I (282) KpnI (286) PacI (303) MluI (309) AscI (316) Eco53kI (327) SacI (329) loxP Kozak sequence NheI (434) BmtI (438) EcoRI (445) SV40 NLS small t intron SV40 poly(A) signal SV40 NLS small t intron SV40 poly(A) signal SV40 NLS SV40 poly(A) signal small t intron AflII (4045) MSCV-rev (4428 .. 4451) AgeI (4503) SpeI (4638) BlpI (4666) StuI (4687) Lox-Stop-Lox TOPO Hygro 9643 bp

Sequence View

5ʹ 3ʹ 60 a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g

120 CAP binding site a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g

180 -35 -10 CAP binding site lac promoter t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t

240 lac operator M13 rev SP6 promoter A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse A T SP6 t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c t a t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g a t a

Acc65IKpnI 300 SP6 promoter T T A G G T G A C A C T A T A G SP6 t t a g g t g a c a c t a t a g a a t a c t c a a g c t a t g c a t c a a g c t t g g t a c c a a t c t c g a g c c t t a a t c c a c t g t g a t a t c t t a t g a g t t c g a t a c g t a g t t c g a a c c a t g g t t a g a g c t c g g a a

Eco53kISacIAscIMluIPacI 360 a a t t a a c c a c g c g t g g c g c g c c t t g a g c t c g g a t c c g g g c c c c c c c t c g a t c g a g g t c g a t t a a t t g g t g c g c a c c g c g c g g a a c t c g a g c c t a g g c c c g g g g g g g a g c t a g c t c c a g c t

420 loxP c a t a a c t t c g t a t a a t g t a t g c t a t a c g a a g t t a t t a g g t c c c t c g a c c t g c a g a c t t a c g t a t t g a a g c a t a t t a c a t a c g a t a t g c t t c a a t a a t c c a g g g a g c t g g a c g t c t g a a t g

EcoRIBmtINheI 480 Kozak sequence SV40 poly(A) signal t c a a g c c a t g g t g g c t a g c t t g c g g a a t t c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g a g t t c g g t a c c a c c g a t c g a a c g c c t t a a g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c

540 SV40 poly(A) signal g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t

600 SV40 poly(A) signal t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g

660 (in frame with SV40 NLS) *TEPEPPPTPPKKFCTFGR *TEPEPPPTPPKKFCTFGR a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a

720 (in frame with SV40 NLS) CIHYPQNHDRTLASYMLYEK CIHYPQNHDRTLASYMLYEK a c a a a t g t g g t a t g g c t g a t t a t g a t c t c t a g t c a a g g c a c t a t a c a t c a a a t a t t c c t t t g t t t a c a c c a t a c c g a c t a a t a c t a g a g a t c a g t t c c g t g a t a t g t a g t t t a t a a g g a a

780 (in frame with SV40 NLS) NVGKCILFSFTCLKQAYNNI NVGKCILFSFTCLKQAYNNI a t t a a c c c c t t t a c a a a t t a a a a a g c t a a a g g t a c a c a a t t t t t g a g c a t a g t t a t t a a t t a a t t g g g g a a a t g t t t a a t t t t t c g a t t t c c a t g t g t t a a a a a c t c g t a t c a a t a a t t a

840 (in frame with SV40 NLS) ASVRHRHPTLFFLINHNYSN ASVRHRHPTLFFLINHNYSN a g c a g a c a c t c t a t g c c t g t g t g g a g t a a g a a a a a a c a g t a t g t t a t g a t t a t a a c t g t t t c g t c t g t g a g a t a c g g a c a c a c c t c a t t c t t t t t t g t c a t a c a a t a c t a a t a t t g a c a a

900 (in frame with SV40 NLS) HRSIFTVSYKEMIKKYLLAA HRSIFTVSYKEMIKKYLLAA a t g c c t a c t t a t a a a g g t t a c a g a a t a t t t t t c c a t a a t t t t c t t g t a t a g c a g t g c a g c t a c g g a t g a a t a t t t c c a a t g t c t t a t a a a a a g g t a t t a a a a g a a c a t a t c g t c a c g t c g

960 (in frame with SV40 NLS) KEKTTYIAFCALTRNSFVAH KEKTTYIAFCALTRNSFVAH t t t t t c c t t t g t g g t g t a a a t a g c a a a g c a a g c a a g a g t t c t a t t a c t a a a c a c a g c a t g a a a a a g g a a a c a c c a c a t t t a t c g t t t c g t t c g t t c t c a a g a t a a t g a t t t g t g t c g t a c

1020 (in frame with SV40 NLS) SLFSLLESPFDKPDE 130 VKRKK SV40 NLS SLFSLLESPFDKPDEVKRKK a c t c a a a a a a c t t a g c a a t t c t g a a g g a a a g t c c t t g g g g t c t t c t a c c t t t c t c t t c t t t g a g t t t t t t g a a t c g t t a a g a c t t c c t t t c a g g a a c c c c a g a a g a t g g a a a g a g a a g a a

1080 KP SV40 NLS KPPTSHQSDATAEDDSSPM t t t t g g a g g a g t a g a a t g t t g a g a g t c a g c a g t a g c c t c a t c a t c a c t a g a t g g c a t t t c a a a a c c t c c t c a t c t t a c a a c t c t c a g t c g t c a t c g g a g t a g t a g t g a t c t a c c g t a a a g

1140 t t c t g a g c a a a a c a g g t t t t c c t c a t t a a a g g c a t t c c a c c a c t g c t c c c a t t c a t c a g t a a g a c t c g t t t t g t c c a a a a g g a g t a a t t t c c g t a a g g t g g t g a c g a g g g t a a g t a g t c a

1200 small t intron t c c a t a g g t t g g a a t c t a a a a t a c a c a a a c a a t t a g a a t c a g t a g t t t a a c a c a t t a t a c a g g t a t c c a a c c t t a g a t t t t a t g t g t t t g t t a a t c t t a g t c a t c a a a t t g t g t a a t a t g

1260 small t intron a c t t a a a a a t t t t a t a t t t a c c t t a g a g c t t t a a a t c t c t g t a g g t a g t t t g t c c a a t t a t g a a t t t t t a a a a t a t a a a t g g a a t c t c g a a a t t t a g a g a c a t c c a t c a a a c a g g t t a a t

1320 SV40 poly(A) signal t g t c a c a c c a c a g a a g t a a g g t t c c t t c a c a a a g a t c c c t c g a c c a g a c a t g a t a a g a t a a c a g t g t g g t g t c t t c a t t c c a a g g a a g t g t t t c t a g g g a g c t g g t c t g t a c t a t t c t a t

1380 SV40 poly(A) signal c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t

1440 SV40 poly(A) signal a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t

1500 (in frame with SV40 NLS) *TEPEPPPTPPKKF *TEPEPPPTPPKKF c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t a a a g g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a a t t t c

1560 (in frame with SV40 NLS) CTFGRCIHYPQNHDRTLASY CTFGRCIHYPQNHDRTLASY c a a g t a a a a c c t c t a c a a a t g t g g t a t g g c t g a t t a t g a t c t c t a g t c a a g g c a c t a t a c g t t c a t t t t g g a g a t g t t t a c a c c a t a c c g a c t a a t a c t a g a g a t c a g t t c c g t g a t a t g

1620 (in frame with SV40 NLS) MLYEKNVGKCILFSFTCLKQ MLYEKNVGKCILFSFTCLKQ a t c a a a t a t t c c t t a t t a a c c c c t t t a c a a a t t a a a a a g c t a a a g g t a c a c a a t t t t t g a t a g t t t a t a a g g a a t a a t t g g g g a a a t g t t t a a t t t t t c g a t t t c c a t g t g t t a a a a a c t

1680 (in frame with SV40 NLS) AYNNIASVRHRHPTLFFLIN AYNNIASVRHRHPTLFFLIN g c a t a g t t a t t a a t a g c a g a c a c t c t a t g c c t g t g t g g a g t a a g a a a a a a c a g t a t g t t a c g t a t c a a t a a t t a t c g t c t g t g a g a t a c g g a c a c a c c t c a t t c t t t t t t g t c a t a c a a t

1740 (in frame with SV40 NLS) HNYSNHRSIFTVSYKEMIKK HNYSNHRSIFTVSYKEMIKK t g a t t a t a a c t g t t a t g c c t a c t t a t a a a g g t t a c a g a a t a t t t t t c c a t a a t t t t c t t g a c t a a t a t t g a c a a t a c g g a t g a a t a t t t c c a a t g t c t t a t a a a a a g g t a t t a a a a g a a c

1800 (in frame with SV40 NLS) YLLAAKEKTTYIAFCALTRN YLLAAKEKTTYIAFCALTRN t a t a g c a g t g c a g c t t t t t c c t t t g t g g t g t a a a t a g c a a a g c a a g c a a g a g t t c t a t t a a t a t c g t c a c g t c g a a a a a g g a a a c a c c a c a t t t a t c g t t t c g t t c g t t c t c a a g a t a a t

1860 (in frame with SV40 NLS) SFVAHSLFSLLESPFDKPDE SV40 NLS SFVAHSLFSLLESPFDKPDE c t a a a c a c a g c a t g a c t c a a a a a a c t t a g c a a t t c t g a a g g a a a g t c c t t g g g g t c t t c t g a t t t g t g t c g t a c t g a g t t t t t t g a a t c g t t a a g a c t t c c t t t c a g g a a c c c c a g a a g a

1920 130 VKRKKKP SV40 NLS VKRKKKPPTSHQSDATAEDD a c c t t t c t c t t c t t t t t t g g a g g a g t a g a a t g t t g a g a g t c a g c a g t a g c c t c a t c a t c a t g g a a a g a g a a g a a a a a a c c t c c t c a t c t t a c a a c t c t c a g t c g t c a t c g g a g t a g t a g t

1980 SSPM c t a g a t g g c a t t t c t t c t g a g c a a a a c a g g t t t t c c t c a t t a a a g g c a t t c c a c c a c t g c g a t c t a c c g t a a a g a a g a c t c g t t t t g t c c a a a a g g a g t a a t t t c c g t a a g g t g g t g a c g

2040 small t intron t c c c a t t c a t c a g t t c c a t a g g t t g g a a t c t a a a a t a c a c a a a c a a t t a g a a t c a g t a g t a g g g t a a g t a g t c a a g g t a t c c a a c c t t a g a t t t t a t g t g t t t g t t a a t c t t a g t c a t c a

2100 small t intron t t a a c a c a t t a t a c a c t t a a a a a t t t t a t a t t t a c c t t a g a g c t t t a a a t c t c t g t a g g t a a t t g t g t a a t a t g t g a a t t t t t a a a a t a t a a a t g g a a t c t c g a a a t t t a g a g a c a t c c a

2160 a g t t t g t c c a a t t a t g t c a c a c c a c a g a a g t a a g g t t c c t t c a c a a a g a t c c c t c g a c c a t c a a a c a g g t t a a t a c a g t g t g g t g t c t t c a t t c c a a g g a a g t g t t t c t a g g g a g c t g g t

2220 SV40 poly(A) signal g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t

2280 SV40 poly(A) signal t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a

2340 (in frame with SV40 NLS) *TEPEPPPTP SV40 poly(A) signal *TEPEPPPTP a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c

2400 (in frame with SV40 NLS) PKKFCTFGRCIHYPQNHDRT PKKFCTFGRCIHYPQNHDRT g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a t g g c t g a t t a t g a t c t c t a g c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t a c c g a c t a a t a c t a g a g a t c

2460 (in frame with SV40 NLS) LASYMLYEKNVGKCILFSFT LASYMLYEKNVGKCILFSFT t c a a g g c a c t a t a c a t c a a a t a t t c c t t a t t a a c c c c t t t a c a a a t t a a a a a g c t a a a g g a g t t c c g t g a t a t g t a g t t t a t a a g g a a t a a t t g g g g a a a t g t t t a a t t t t t c g a t t t c c

2520 (in frame with SV40 NLS) CLKQAYNNIASVRHRHPTLF CLKQAYNNIASVRHRHPTLF t a c a c a a t t t t t g a g c a t a g t t a t t a a t a g c a g a c a c t c t a t g c c t g t g t g g a g t a a g a a a t g t g t t a a a a a c t c g t a t c a a t a a t t a t c g t c t g t g a g a t a c g g a c a c a c c t c a t t c t t

2580 (in frame with SV40 NLS) FLINHNYSNHRSIFTVSYKE FLINHNYSNHRSIFTVSYKE a a a a c a g t a t g t t a t g a t t a t a a c t g t t a t g c c t a c t t a t a a a g g t t a c a g a a t a t t t t t t t t t g t c a t a c a a t a c t a a t a t t g a c a a t a c g g a t g a a t a t t t c c a a t g t c t t a t a a a a a

2640 (in frame with SV40 NLS) MIKKYLLAAKEKTTYIAFCA MIKKYLLAAKEKTTYIAFCA c c a t a a t t t t c t t g t a t a g c a g t g c a g c t t t t t c c t t t g t g g t g t a a a t a g c a a a g c a a g g g t a t t a a a a g a a c a t a t c g t c a c g t c g a a a a a g g a a a c a c c a c a t t t a t c g t t t c g t t c

2700 (in frame with SV40 NLS) LTRNSFVAHSLFSLLESPFD LTRNSFVAHSLFSLLESPFD c a a g a g t t c t a t t a c t a a a c a c a g c a t g a c t c a a a a a a c t t a g c a a t t c t g a a g g a a a g t g t t c t c a a g a t a a t g a t t t g t g t c g t a c t g a g t t t t t t g a a t c g t t a a g a c t t c c t t t c a

2760 KPDE 130 VKRKKKP SV40 NLS KPDEVKRKKKPPTSHQSDAT c c t t g g g g t c t t c t a c c t t t c t c t t c t t t t t t g g a g g a g t a g a a t g t t g a g a g t c a g c a g g g a a c c c c a g a a g a t g g a a a g a g a a g a a a a a a c c t c c t c a t c t t a c a a c t c t c a g t c g t c

2820 AEDDSSPM t a g c c t c a t c a t c a c t a g a t g g c a t t t c t t c t g a g c a a a a c a g g t t t t c c t c a t t a a a g g a t c g g a g t a g t a g t g a t c t a c c g t a a a g a a g a c t c g t t t t g t c c a a a a g g a g t a a t t t c c

2880 small t intron c a t t c c a c c a c t g c t c c c a t t c a t c a g t t c c a t a g g t t g g a a t c t a a a a t a c a c a a a c a a g t a a g g t g g t g a c g a g g g t a a g t a g t c a a g g t a t c c a a c c t t a g a t t t t a t g t g t t t g t t

2940 small t intron t t a g a a t c a g t a g t t t a a c a c a t t a t a c a c t t a a a a a t t t t a t a t t t a c c t t a g a g c t t t a a t c t t a g t c a t c a a a t t g t g t a a t a t g t g a a t t t t t a a a a t a t a a a t g g a a t c t c g a a a

3000 a a a t c t c t g t a g g t a g t t t g t c c a a t t a t g t c a c a c c a c a g a a g t a a g g t t c c t t c a c a a t t t a g a g a c a t c c a t c a a a c a g g t t a a t a c a g t g t g g t g t c t t c a t t c c a a g g a a g t g t t

3060 SV40 poly(A) signal a g a t c c c t c g a c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t c t a g g g a g c t g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t

3120 SV40 poly(A) signal t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t

3180 (in frame with SV40 NLS) *TEPE SV40 poly(A) signal *TEPE t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g

3240 (in frame with SV40 NLS) PPPTPPKKFCTFGRCIHYPQ PPPTPPKKFCTFGRCIHYPQ a g g g g g a g g t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a t g g c t g t c c c c c t c c a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t a c c g a c

3300 (in frame with SV40 NLS) NHDRTLASYMLYEKNVGKCI NHDRTLASYMLYEKNVGKCI a t t a t g a t c t c t a g t c a a g g c a c t a t a c a t c a a a t a t t c c t t a t t a a c c c c t t t a c a a a t t a a t a c t a g a g a t c a g t t c c g t g a t a t g t a g t t t a t a a g g a a t a a t t g g g g a a a t g t t t a

3360 (in frame with SV40 NLS) LFSFTCLKQAYNNIASVRHR LFSFTCLKQAYNNIASVRHR t a a a a a g c t a a a g g t a c a c a a t t t t t g a g c a t a g t t a t t a a t a g c a g a c a c t c t a t g c c t a t t t t t c g a t t t c c a t g t g t t a a a a a c t c g t a t c a a t a a t t a t c g t c t g t g a g a t a c g g a

3420 (in frame with SV40 NLS) HPTLFFLINHNYSNHRSIFT HPTLFFLINHNYSNHRSIFT g t g t g g a g t a a g a a a a a a c a g t a t g t t a t g a t t a t a a c t g t t a t g c c t a c t t a t a a a g g t c a c a c c t c a t t c t t t t t t g t c a t a c a a t a c t a a t a t t g a c a a t a c g g a t g a a t a t t t c c a

3480 (in frame with SV40 NLS) VSYKEMIKKYLLAAKEKTTY VSYKEMIKKYLLAAKEKTTY t a c a g a a t a t t t t t c c a t a a t t t t c t t g t a t a g c a g t g c a g c t t t t t c c t t t g t g g t g t a a t g t c t t a t a a a a a g g t a t t a a a a g a a c a t a t c g t c a c g t c g a a a a a g g a a a c a c c a c a t

3540 (in frame with SV40 NLS) IAFCALTRNSFVAHSLFSLL IAFCALTRNSFVAHSLFSLL a a t a g c a a a g c a a g c a a g a g t t c t a t t a c t a a a c a c a g c a t g a c t c a a a a a a c t t a g c a a t t a t c g t t t c g t t c g t t c t c a a g a t a a t g a t t t g t g t c g t a c t g a g t t t t t t g a a t c g t t

3600 (in frame with SV40 NLS) ESPFDKPDE 130 VKRKKKP SV40 NLS ESPFDKPDEVKRKKKPPTSH t t c t g a a g g a a a g t c c t t g g g g t c t t c t a c c t t t c t c t t c t t t t t t g g a g g a g t a g a a t g a a g a c t t c c t t t c a g g a a c c c c a g a a g a t g g a a a g a g a a g a a a a a a c c t c c t c a t c t t a c

3660 QSDATAEDDSSPM t t g a g a g t c a g c a g t a g c c t c a t c a t c a c t a g a t g g c a t t t c t t c t g a g c a a a a c a g g t t a a c t c t c a g t c g t c a t c g g a g t a g t a g t g a t c t a c c g t a a a g a a g a c t c g t t t t g t c c a a

3720 small t intron t t c c t c a t t a a a g g c a t t c c a c c a c t g c t c c c a t t c a t c a g t t c c a t a g g t t g g a a t c t a a a g g a g t a a t t t c c g t a a g g t g g t g a c g a g g g t a a g t a g t c a a g g t a t c c a a c c t t a g a t

3780 small t intron a a a t a c a c a a a c a a t t a g a a t c a g t a g t t t a a c a c a t t a t a c a c t t a a a a a t t t t a t a t t t t t a t g t g t t t g t t a a t c t t a g t c a t c a a a t t g t g t a a t a t g t g a a t t t t t a a a a t a t a a

3840 small t intron t a c c t t a g a g c t t t a a a t c t c t g t a g g t a g t t t g t c c a a t t a t g t c a c a c c a c a g a a g t a a t g g a a t c t c g a a a t t t a g a g a c a t c c a t c a a a c a g g t t a a t a c a g t g t g g t g t c t t c a t

3900 a g g t t c c t t c a c a a a g a t c c c t c g a t c g a g a a a a a a a a t a t a a a a g a g a t g g a g g a a c g g t c c a a g g a a g t g t t t c t a g g g a g c t a g c t c t t t t t t t t a t a t t t t c t c t a c c t c c t t g c c

3960 g a a a a a g t t a g t t g t g g t g a t a g g t g g c a a g t g g t a t t c c g t a a g a a c a a c a a g a a a a g c c t t t t t c a a t c a a c a c c a c t a t c c a c c g t t c a c c a t a a g g c a t t c t t g t t g t t c t t t t c g

4020 MAELSEQVQNLSINDNN a t t t c a t a t t a t g g c t g a a c t g a g c g a a c a a g t g c a a a a t t t a a g c a t c a a c g a c a a c a a t a a a g t a t a a t a c c g a c t t g a c t c g c t t g t t c a c g t t t t a a a t t c g t a g t t g c t g t t g t t

AflII 4080 ENGYVPPHLRGKPRSARNNM c g a g a a t g g t t a t g t t c c t c c t c a c t t a a g a g g a a a a c c a a g a a g t g c c a g a a a t a a c a t g c t c t t a c c a a t a c a a g g a g g a g t g a a t t c t c c t t t t g g t t c t t c a c g g t c t t t a t t g t a

4140 SNYNNNNGGYNGGRGGGSFF g a g c a a c t a c a a t a a c a a c a a c g g c g g c t a c a a c g g t g g c c g t g g c g g t g g c a g c t t c t t c t c g t t g a t g t t a t t g t t g t t g c c g c c g a t g t t g c c a c c g g c a c c g c c a c c g t c g a a g a a

4200 SNNRRGGYGNGGFFGGNNGG t a g c a a c a a c c g t c g t g g t g g t t a c g g c a a c g g t g g t t t c t t c g g t g g a a a c a a c g g t g g a t c g t t g t t g g c a g c a c c a c c a a t g c c g t t g c c a c c a a a g a a g c c a c c t t t g t t g c c a c c

4260 SRSNGRSGGRWIDGKHVPAP c a g c a g a t c t a a c g g c c g t t c t g g t g g t a g a t g g a t c g a t g g c a a a c a t g t c c c a g c t c c g t c g t c t a g a t t g c c g g c a a g a c c a c c a t c t a c c t a g c t a c c g t t t g t a c a g g g t c g a g g

4320 RNEKAEIAIFGVPEDPCGKK a a g a a a c g a a a a g g c c g a g a t c g c c a t a t t t g g t g t c c c c g a g g a t c c c t g t g g a a a a a a t t c t t t g c t t t t c c g g c t c t a g c g g t a t a a a c c a c a g g g g c t c c t a g g g a c a c c t t t t t t

4380 RDRISMTSSRKPWTTAPFDL a a g g g a c a g g a t a a g t a t g a c a t c a t c a a g g a a a c c c t g g a c t a c t g c g c c c t t c g a c c t t t c c c t g t c c t a t t c a t a c t g t a g t a g t t c c t t t g g g a c c t g a t g a c g c g g g a a g c t g g a

4440 PGK promoter C G T A C G C G A A A T C MSCV-rev EILPGRGGAFPKAVWSMRFS c g a a a t t c t a c c g g g t a g g g g a g g c g c t t t t c c c a a g g c a g t c t g g a g c a t g c g c t t t a g g c t t t a a g a t g g c c c a t c c c c t c c g c g a a a a g g g t t c c g t c a g a c c t c g t a c g c g a a a t c

4500 PGK promoter G T C G G G G C G A C MSCV-rev SPAGHLALHKWPLASHTFHI c a g c c c c g c t g g g c a c t t g g c g c t a c a c a a g t g g c c t c t g g c c t c g c a c a c a t t c c a c a t g t c g g g g c g a c c c g t g a a c c g c g a t g t g t t c a c c g g a g a c c g g a g c g t g t g t a a g g t g t a

AgeI 4560 PGK promoter HR* c c a c c g g t a g g c g c c a a c c g g c t c c g t t c t t t g g t g g c c c c t t c g c g c c a c c t t c t a c t c g g t g g c c a t c c g c g g t t g g c c g a g g c a a g a a a c c a c c g g g g a a g c g c g g t g g a a g a t g a g

4620 PGK promoter c t c c c c t a g t c a g g a a g t t c c c c c c c g c c c c g c a g c t c g c g t c g t g c a g g a c g t g a c a a a g a g g g g a t c a g t c c t t c a a g g g g g g g c g g g g c g t c g a g c g c a g c a c g t c c t g c a c t g t t t

BlpISpeI 4680 PGK promoter t g g a a g t a g c a c g t c t c a c t a g t c t c g t g c a g a t g g a c a g c a c c g c t g a g c a a t g g a a g c a c c t t c a t c g t g c a g a g t g a t c a g a g c a c g t c t a c c t g t c g t g g c g a c t c g t t a c c t t c g

StuI 4740 PGK promoter g g g t a g g c c t t t g g g g c a g c g g c c a a t a g c a g c t t t g c t c c t t c g c t t t c t g g g c t c a g a c c c a t c c g g a a a c c c c g t c g c c g g t t a t c g t c g a a a c g a g g a a g c g a a a g a c c c g a g t c t

4800 PGK promoter *PRPPR g g c t g g g a a g g g g t g g g t c c g g g g g c g g g c t c a g g g g c g g g c t c a g g g g c g g g g c g g g c g c c g a c c c t t c c c c a c c c a g g c c c c c g c c c g a g t c c c c g c c c g a g t c c c c g c c c c g c c c g c

4860 PGK promoter C A T T C T G C A C G C T T C A A A A G mPGK-F GFTRRLGPMRCAEFACTQRA c c c g a a g g t c c t c c g g a g g c c c g g c a t t c t g c a c g c t t c a a a a g c g c a c g t c t g c c g c g c g g g c t t c c a g g a g g c c t c c g g g c c g t a a g a c g t g c g a a g t t t t c g c g t g c a g a c g g c g c g

4920 PGK promoter TRRKRMEPGKSRCGDLDRDL t g t t c t c c t c t t c c t c a t c t c c g g g c c t t t c g a c c t g c a t c c a t c t a g a t c t c g a t c g a g a c a a g a g g a g a a g g a g t a g a g g c c c g g a a a g c t g g a c g t a g g t a g a t c t a g a g c t a g c t c

PshAI 4980 1 5 10 15 MKKPELTATSVEKFL HygR 1 5 10 15 MKKPELTATSVEKFL HygR MKKPELTATSVEKFL LQLKGHFLRFEGRRRDLLKQ c a g c t g a a g c t t a c c a t g a a a a a g c c t g a a c t c a c c g c g a c g t c t g t c g a g a a g t t t c t g g t c g a c t t c g a a t g g t a c t t t t t c g g a c t t g a g t g g c g c t g c a g a c a g c t c t t c a a a g a c

5040 20 25 30 35 IEKFDSVSDLMQLSEGEESR HygR 20 25 30 35 IEKFDSVSDLMQLSEGEESR HygR IEKFDSVSDLMQLSEGEESR DFLEVADGVQHLERLAFFRT a t c g a a a a g t t c g a c a g c g t c t c c g a c c t g a t g c a g c t c t c g g a g g g c g a a g a a t c t c g t t a g c t t t t c a a g c t g t c g c a g a g g c t g g a c t a c g t c g a g a g c c t c c c g c t t c t t a g a g c a

5100 40 45 50 55 AFSFDVGGRGYVLRVNSCAD HygR 40 45 50 55 AFSFDVGGRGYVLRVNSCAD HygR AFSFDVGGRGYVLRVNSCAD SEAEIYSPTSIDQPYIAAGI g c t t t c a g c t t c g a t g t a g g a g g g c g t g g a t a t g t c c t g c g g g t a a a t a g c t g c g c c g a t c g a a a g t c g a a g c t a c a t c c t c c c g c a c c t a t a c a g g a c g c c c a t t t a t c g a c g c g g c t a

5160 60 65 70 75 GFYKDRYVYRHFASAALPIP HygR 60 65 70 75 GFYKDRYVYRHFASAALPIP HygR GFYKDRYVYRHFASAALPIP TEVFITINIPVKCRGRERNR g g t t t c t a c a a a g a t c g t t a t g t t t a t c g g c a c t t t g c a t c g g c c g c g c t c c c g a t t c c g c c a a a g a t g t t t c t a g c a a t a c a a a t a g c c g t g a a a c g t a g c c g g c g c g a g g g c t a a g g c

5220 80 85 90 95 EVLDIGEFSESLTYCISRRA HygR 80 85 90 95 EVLDIGEFSESLTYCISRRA HygR EVLDIGEFSESLTYCISRRA FHKVNPFKALAQGIADGATC g a a g t g c t t g a c a t t g g g g a a t t t a g c g a g a g c c t g a c c t a t t g c a t c t c c c g c c g t g c a c t t c a c g a a c t g t a a c c c c t t a a a t c g c t c t c g g a c t g g a t a a c g t a g a g g g c g g c a c g t

5280 100 105 110 115 QGVTLQDLPETELPAVLQPV HygR 100 105 110 115 QGVTLQDLPETELPAVLQPV HygR QGVTLQDLPETELPAVLQPV LTDRQLVQRFGFQGSNQLRD c a g g g t g t c a c g t t g c a a g a c c t g c c t g a a a c c g a a c t g c c c g c t g t t c t g c a g c c g g t c g t c c c a c a g t g c a a c g t t c t g g a c g g a c t t t g g c t t g a c g g g c g a c a a g a c g t c g g c c a g

AsiSI 5340 120 125 130 135 AEAMDAIAAADLSQTSGFGP HygR 120 125 130 135 AEAMDAIAAADLSQTSGFGP HygR AEAMDAIAAADLSQTSGFGP RLGHIRDSRGIKALRAPEAW g c g g a g g c c a t g g a t g c g a t c g c t g c g g c c g a t c t t a g c c a g a c g a g c g g g t t c g g c c c a c g c c t c c g g t a c c t a c g c t a g c g a c g c c g g c t a g a a t c g g t c t g c t c g c c c a a g c c g g g t

NdeI 5400 140 145 150 155 FGPQGIGQYTTWRDFICAIA HygR 140 145 150 155 FGPQGIGQYTTWRDFICAIA HygR FGPQGIGQYTTWRDFICAIA ESRLSDTLVSCPTIEYARNS t t c g g a c c g c a a g g a a t c g g t c a a t a c a c t a c a t g g c g t g a t t t c a t a t g c g c g a t t g c t a a g c c t g g c g t t c c t t a g c c a g t t a t g t g a t g t a c c g c a c t a a a g t a t a c g c g c t a a c g a

5460 160 165 170 175 DPHVYHWQTVMDDTVSASVA HygR 160 165 170 175 DPHVYHWQTVMDDTVSASVA HygR DPHVYHWQTVMDDTVSASVA IGMHIVPLSHHVVGDTRGDR g a t c c c c a t g t g t a t c a c t g g c a a a c t g t g a t g g a c g a c a c c g t c a g t g c g t c c g t c g c g c t a g g g g t a c a c a t a g t g a c c g t t t g a c a c t a c c t g c t g t g g c a g t c a c g c a g g c a g c g c

5520 180 185 190 195 QALDELMLWAEDCPEVRHLV HygR 180 185 190 195 QALDELMLWAEDCPEVRHLV HygR QALDELMLWAEDCPEVRHLV LSEILQHKPGLVAGFDPVEH c a g g c t c t c g a t g a g c t g a t g c t t t g g g c c g a g g a c t g c c c c g a a g t c c g g c a c c t c g t g g t c c g a g a g c t a c t c g a c t a c g a a a c c c g g c t c c t g a c g g g g c t t c a g g c c g t g g a g c a c

5580 200 205 210 215 HADFGSNNVLTDNGRITAVI HygR 200 205 210 215 HADFGSNNVLTDNGRITAVI HygR HADFGSNNVLTDNGRITAVI VRIEAGVIDQRVIAAYCRDN c a c g c g g a t t t c g g c t c c a a c a a t g t c c t g a c g g a c a a t g g c c g c a t a a c a g c g g t c a t t g t g c g c c t a a a g c c g a g g t t g t t a c a g g a c t g c c t g t t a c c g g c g t a t t g t c g c c a g t a a

5640 220 225 230 235 DWSEAMFGDSQYEVANIFFW HygR 220 225 230 235 DWSEAMFGDSQYEVANIFFW HygR DWSEAMFGDSQYEVANIFFW VPALRHEPIGLVLDGVDEEP g a c t g g a g c g a g g c g a t g t t c g g g g a t t c c c a a t a c g a g g t c g c c a a c a t c t t c t t c t g g c t g a c c t c g c t c c g c t a c a a g c c c c t a a g g g t t a t g c t c c a g c g g t t g t a g a a g a a g a c c

5700 240 245 250 255 RPWLACMEQQTRYFERRHPE HygR 240 245 250 255 RPWLACMEQQTRYFERRHPE HygR RPWLACMEQQTRYFERRHPE PRPQSTHLLLRAVELPPM a g g c c g t g g t t g g c t t g t a t g g a g c a g c a g a c g c g c t a c t t c g a g c g g a g g c a t c c g g a g t c c g g c a c c a a c c g a a c a t a c c t c g t c g t c t g c g c g a t g a a g c t c g c c t c c g t a g g c c t c

SacII 5760 260 265 270 275 LAGSPRLRAYMLRIGLDQLY HygR 260 265 270 275 LAGSPRLRAYMLRIGLDQLY HygR LAGSPRLRAYMLRIGLDQLY c t t g c a g g a t c g c c g c g g c t c c g g g c g t a t a t g c t c c g c a t t g g t c t t g a c c a a c t c t a t g a a c g t c c t a g c g g c g c c g a g g c c c g c a t a t a c g a g g c g t a a c c a g a a c t g g t t g a g a t a

5820 280 285 290 295 QSLVDGNFDDAAWAQGRCDA HygR 280 285 290 295 QSLVDGNFDDAAWAQGRCDA HygR QSLVDGNFDDAAWAQGRCDA c a g a g c t t g g t t g a c g g c a a t t t c g a t g a t g c a g c t t g g g c g c a g g g t c g a t g c g a c g c a g t c t c g a a c c a a c t g c c g t t a a a g c t a c t a c g t c g a a c c c g c g t c c c a g c t a c g c t g c g t

5880 300 305 310 315 IVRSGAGTVGRTQIARRSAA HygR 300 305 310 315 IVRSGAGTVGRTQIARRSAA HygR IVRSGAGTVGRTQIARRSAA a t c g t c c g a t c c g g a g c c g g g a c t g t c g g g c g t a c a c a a a t c g c c c g c a g a a g c g c g g c c t a g c a g g c t a g g c c t c g g c c c t g a c a g c c c g c a t g t g t t t a g c g g g c g t c t t c g c g c c g g

5940 320 325 330 335 VWTDGCVEVLADSGNRRPST HygR 320 325 330 335 VWTDGCVEVLADSGNRRPST HygR VWTDGCVEVLADSGNRRPST g t c t g g a c c g a t g g c t g t g t a g a a g t a c t c g c c g a t a g t g g a a a c c g a c g c c c c a g c a c t c a g a c c t g g c t a c c g a c a c a t c t t c a t g a g c g g c t a t c a c c t t t g g c t g c g g g g t c g t g a

6000 340 RPRAKE* HygR 340 RPRAKE* HygR RPRAKE* c g t c c g a g g g c a a a g g a a t a g a g t a t c g a t g a t a t c a g a t c c c c g g g a t g c a g a a a t t g a g c a g g c t c c c g t t t c c t t a t c t c a t a g c t a c t a t a g t c t a g g g g c c c t a c g t c t t t a a c t

XmnI 6060 t g a t c t a t t a a a c a a t a a a g a t g t c c a c t a a a a t g g a a g t t t t t c c t g t c a t a c t t t g t t a c t a g a t a a t t t g t t a t t t c t a c a g g t g a t t t t a c c t t c a a a a a g g a c a g t a t g a a a c a a

6120 a a g a a g g g t g a g a a c a g a g t a c c t a c a t t t t g a a t g g a a g g a t t g g a g c t a c g g g g g t g g t t c t t c c c a c t c t t g t c t c a t g g a t g t a a a a c t t a c c t t c c t a a c c t c g a t g c c c c c a c c

6180 g g g t g g g g t g g g a t t a g a t a a a t g c c t g c t c t t t a c t g a a g g c t c t t t a c t a t t g c t t t a c c c a c c c c a c c c t a a t c t a t t t a c g g a c g a g a a a t g a c t t c c g a g a a a t g a t a a c g a a a t

6240 t g a t a a t g t t t c a t a g t t g g a t a t c a t a a t t t a a a c a a g c a a a a c c a a a t t a a g g g c c a g a c t a t t a c a a a g t a t c a a c c t a t a g t a t t a a a t t t g t t c g t t t t g g t t t a a t t c c c g g t c

6300 c t c a t t c c t c c c a c t c a t g a t c t a t a g a t c t a t a g a t c t c t c g t g g g a t c a t t g t t t t t c g a g t a a g g a g g g t g a g t a c t a g a t a t c t a g a t a t c t a g a g a g c a c c c t a g t a a c a a a a a g

6360 t c t t g a t t c c c a c t t t g t g g t t c t a a g t a c t g t g g t t t c c a a a t g t g t c a g t t t c a t a g c a g a a c t a a g g g t g a a a c a c c a a g a t t c a t g a c a c c a a a g g t t t a c a c a g t c a a a g t a t c g

6420 c t g a a g a a c g a g a t c a g c a g c c t c t g t t c c a c a t a c a c t t c a t t c t c a g t a t t g t t t t g c g a c t t c t t g c t c t a g t c g t c g g a g a c a a g g t g t a t g t g a a g t a a g a g t c a t a a c a a a a c g

6480 loxP c a a g t t c t a a t t c c a t c a g a a g c t g g t c g a g a t a a c t t c g t a t a a t g t a t g c t a t a c g a a g t t c a a g a t t a a g g t a g t c t t c g a c c a g c t c t a t t g a a g c a t a t t a c a t a c g a t a t g c t t

PspXINotI 6540 loxP T7 promoter G G G A T A T C A C T T7 g t t a t g t c g a c a g c g g c c g c t c g a g c a t g c a t c t a g a g g g c c c a a t t c g c c c t a t a g t g a c a a t a c a g c t g t c g c c g g c g a g c t c g t a c g t a g a t c t c c c g g g t t a a g c g g g a t a t c a c t

6600 T7 promoter M13 fwd C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward g t c g t a t t a c a a t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t c a g c a t a a t g t t a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a

6660 t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t

SnaBIBsiWI 6720 5 QFKV ccdB g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t a t a c g t a c g g c a g t t t a a g g t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a t a t g c a t g c c g t c a a a t t c c a

BsrGI 6780 10 15 20 25 YTYKRESRYRLFVDVQSDII ccdB t t a c a c c t a t a a a a g a g a g a g c c g t t a t c g t c t g t t t g t g g a t g t a c a g a g t g a t a t t a t a a t g t g g a t a t t t t c t c t c t c g g c a a t a g c a g a c a a a c a c c t a c a t g t c t c a c t a t a a t a

6840 30 35 40 45 DTPGRRMVIPLASARLLSDK ccdB t g a c a c g c c g g g g c g a c g g a t g g t g a t c c c c c t g g c c a g t g c a c g t c t g c t g t c a g a t a a a c t g t g c g g c c c c g c t g c c t a c c a c t a g g g g g a c c g g t c a c g t g c a g a c g a c a g t c t a t t

6900 50 55 60 65 VSRELYPVVHIGDESWRMMT ccdB G C A T G A T G A C ccdB-fwd a g t c t c c c g t g a a c t t t a c c c g g t g g t g c a t a t c g g g g a t g a a a g c t g g c g c a t g a t g a c t c a g a g g g c a c t t g a a a t g g g c c a c c a c g t a t a g c c c c t a c t t t c g a c c g c g t a c t a c t g

BsaI 6960 70 75 80 85 TDMASVPVSVIGEEVADLSH ccdB C A C C G A T A T G ccdB-fwd c a c c g a t a t g g c c a g t g t g c c g g t c t c c g t t a t c g g g g a a g a a g t g g c t g a t c t c a g c c a g t g g c t a t a c c g g t c a c a c g g c c a g a g g c a a t a g c c c c t t c t t c a c c g a c t a g a g t c g g t

7020 90 95 100 RENDIKNAINLMFWGI* ccdB c c g c g a a a a t g a c a t c a a a a a c g c c a t t a a c c t g a t g t t c t g g g g a a t a t a a a t g t c a g g g g c g c t t t t a c t g t a g t t t t t g c g g t a a t t g g a c t a c a a g a c c c c t t a t a t t t a c a g t c c

7080 c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t c a c g t a g a a a g c c a g t c c g c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a g t g c a t c t t t c g g t c a g g c g t

7140 g a a a c g g t g c t g a c c c c g g a t g a a t g t c a g c t a c t g g g c t a t c t g g a c a a g g g a a a a c g c c t t t g c c a c g a c t g g g g c c t a c t t a c a g t c g a t g a c c c g a t a g a c c t g t t c c c t t t t g c g

7200 a a g c g c a a a g a g a a a g c a g g t a g c t t g c a g t g g g c t t a c a t g g c g a t a g c t a g a c t g g g c t t c g c g t t t c t c t t t c g t c c a t c g a a c g t c a c c c g a a t g t a c c g c t a t c g a t c t g a c c c g

7260 g g t t t t a t g g a c a g c a a g c g a a c c g g a a t t g c c a g c t g g g g c g c c c t c t g g t a a g g t t g g c c a a a a t a c c t g t c g t t c g c t t g g c c t t a a c g g t c g a c c c c g c g g g a g a c c a t t c c a a c c

XcmI 7320 g a a g c c c t g c a a a g t a a a c t g g a t g g c t t t c t c g c c g c c a a g g a t c t g a t g g c g c a g g g g c t t c g g g a c g t t t c a t t t g a c c t a c c g a a a g a g c g g c g g t t c c t a g a c t a c c g c g t c c c c

BclI* 7380 1 5 MIEQDGL NeoR/KanR 1 5 MIEQDGL NeoR/KanR MIEQDGL a t c a a g c t c t g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t t a g t t c g a g a c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a

7440 10 15 20 25 HAGSPAAWVERLFGYDWAQQ NeoR/KanR 10 15 20 25 HAGSPAAWVERLFGYDWAQQ NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R HAGSPAAWVERLFGYDWAQQ g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t

7500 30 35 40 45 TIGCSDAAVFRLSAQGRPVL NeoR/KanR 30 35 40 45 TIGCSDAAVFRLSAQGRPVL NeoR/KanR TIGCSDAAVFRLSAQGRPVL g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a

7560 50 55 60 65 FVKTDLSGALNELQDEAARL NeoR/KanR 50 55 60 65 FVKTDLSGALNELQDEAARL NeoR/KanR FVKTDLSGALNELQDEAARL t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a

7620 70 75 80 85 SWLATTGVPCAAVLDVVTEA NeoR/KanR 70 75 80 85 SWLATTGVPCAAVLDVVTEA NeoR/KanR SWLATTGVPCAAVLDVVTEA a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g

7680 90 95 100 105 GRDWLLLGEVPGQDLLSSHL NeoR/KanR 90 95 100 105 GRDWLLLGEVPGQDLLSSHL NeoR/KanR GRDWLLLGEVPGQDLLSSHL *RVK g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a

7740 110 115 120 125 APAEKVSIMADAMRRLHTLD NeoR/KanR 110 115 120 125 APAEKVSIMADAMRRLHTLD NeoR/KanR APAEKVSIMADAMRRLHTLD SRGLFYGDHSICHPPQMRKI t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t

7800 130 135 140 145 PATCPFDHQAKHRIERARTR NeoR/KanR 130 135 140 145 PATCPFDHQAKHRIERARTR NeoR/KanR PATCPFDHQAKHRIERARTR RSGAWEVVLRFMADLSCTSP t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c

7860 150 155 160 165 MEAGLVDQDDLDEEHQGLAP NeoR/KanR 150 155 160 165 MEAGLVDQDDLDEEHQGLAP NeoR/KanR MEAGLVDQDDLDEEHQGLAP HFGTKDILIIQVFLMLPERW g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g

7920 170 175 180 185 AELFARLKASMPDGEDLVVT NeoR/KanR 170 175 180 185 AELFARLKASMPDGEDLVVT NeoR/KanR AELFARLKASMPDGEDLVVT GFQEGPELRAHGVALIEDHG a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g

7980 190 195 200 205 HGDACLPNIMVENGRFSGFI NeoR/KanR 190 195 200 205 HGDACLPNIMVENGRFSGFI NeoR/KanR HGDACLPNIMVENGRFSGFI MAIGAQRIDHHFIAAKRSED c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a

8040 210 215 220 225 DCGRLGVADRYQDIALATRD NeoR/KanR 210 215 220 225 DCGRLGVADRYQDIALATRD NeoR/KanR C G T T G G C T A C C C G T G A Neo-F DCGRLGVADRYQDIALATRD VTAPQTHRVAILVYRQSGTI c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t

8100 230 235 240 245 IAEELGGEWADRFLVLYGIA NeoR/KanR 230 235 240 245 IAEELGGEWADRFLVLYGIA NeoR/KanR T A T T Neo-F IAEELGGEWADRFLVLYGIA NSFLKAAFPSVAEEHKVTDG t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g

8160 250 255 260 265 APDSQRIAFYRLLDEFF* NeoR/KanR 250 255 260 265 APDSQRIAFYRLLDEFF* NeoR/KanR APDSQRIAFYRLLDEFF* SGIRLADGEIAKKVLEESNN c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a a t t a t g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t t a a t a

8220 VSVIEQHPIKEKRM t a a c g c t t a c a a t t t c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a a t t g c g a a t g t t a a a g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t

8280 c c g c a t a c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c g g c g t a t g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g

8340 t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t

MauBISfiI 8400 1 5 10 MAKLTSAVPVLTA BleoR Kozak sequence t a g c a c g t g a g g a g g g c c a c c a t g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t c a c c g c g a t c g t g c a c t c c t c c c g g t g g t a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a g t g g c g c

8460 15 20 25 30 RDVAGAVEFWTDRLGFSRDF BleoR c g c g a c g t c g c c g g a g c g g t c g a g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g g a c t t c g c g c t g c a g c g g c c t c g c c a g c t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c c c t g a a g

SgrAI 8520 35 40 45 50 VEDDFAGVVRDDVTLFISAV BleoR g t g g a g g a c g a c t t c g c c g g t g t g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c g c g g t c c a c c t c c t g c t g a a g c g g c c a c a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c g c g c c a g

SexAI*CsiI 8580 55 60 65 70 QDQVVPDNTLAWVWVRGLDE BleoR c a g g a c c a g g t g g t g c c g g a c a a c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g g a c g a g g t c c t g g t c c a c c a c g g c c t g t t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a c c t g c t c

FseI 8640 75 80 85 90 LYAEWSEVVSTNFRDASGPA BleoR c t g t a c g c c g a g t g g t c g g a g g t c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g g c c g g c c g a c a t g c g g c t c a c c a g c c t c c a g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c c g g c c g g

8700 95 100 105 110 MTEIGEQPWGREFALRDPAG BleoR a t g a c c g a g a t c g g c g a g c a g c c g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c g g c c g g c t a c t g g c t c t a g c c g c t c g t c g g c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g c c g g c c g

8760 115 120 125 NCVHFVAEEQD* BleoR a a c t g c g t g c a c t t c g t g g c c g a g g a g c a g g a c t g a c a c g t g c t a a a a c t t c a t t t t t a a t t g a c g c a c g t g a a g c a c c g g c t c c t c g t c c t g a c t g t g c a c g a t t t t g a a g t a a a a a t t

8820 t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a

8880 ori g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a

8940 ori c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c

9000 ori g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t

9060 ori g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g

9120 ori t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a

9180 ori g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c

9240 ori c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g

9300 ori g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c

9360 ori g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t

9420 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a

9480 ori c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g

9540 t t t t t a c g g t t c c t g g g c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c a a a a a t g c c a a g g a c c c g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g

9600 c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g

3ʹ 5ʹ 9643 A G C G A G T C A G T G A G C G A G L4440 c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.