Addgene His-GST-ATG13 (363-517) Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: His-GST-ATG13 (363-517) Sequencing Result


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tet promoter BspDI - ClaI (5904) HindIII (5897) BmtI (5701) NheI (5697) T7 Term (5466 .. 5484) BlpI (5469) MluI (5404) KpnI (5402) Acc65I (5398) FseI (5395) DraIII (5387) CsiI - SexAI * (5383) AscI (5376) AsiSI (5371) BamHI (5361) BclI * (4617) SwaI (4610) BstBI (4580) BstXI (4171) 6xHis NdeI (4142) XbaI (4102) SbfI (4091) NotI (4079) PacI (4074) SacI (4067) Eco53kI (4065) T7 (4007 .. 4026) BglII (3987) SgrAI (3946) pBRrevBam (3904 .. 3923) SphI (3798) SalI (3705) PshAI (3644) NruI (3386) BstAPI (3312) BfuAI - BspMI (3302) BsmI (3003) pBRforEco (18 .. 36) ZraI (75) AatII (77) Amp-R (427 .. 446) AlwNI (1475) pBR322ori-F (1729 .. 1748) L4440 (1982 .. 1999) pRS-marker (2079 .. 2098) BstZ17I (2114) BsaAI (2133) PflFI - Tth111I (2139) pGEX 3' (2239 .. 2261) BsmBI - Esp3I (2240) PvuII (2294) His-GST-ATG13 (363-517) 5930 bp

Sequence View

5ʹ 3ʹ 60 C G G A G C A C T A T G C G G A T A A pBRforEco t t c t t g a a g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a g a a c t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a

ZraIAatII 120 AmpR promoter a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c

180 AmpR promoter t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a

240 1 5 10 signal sequence MSIQHFRVALI AmpR promoter AmpR MSIQHFRVALI g c t t c a a t a a c a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t c g a a g t t a t t g t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a

300 15 20 signal sequence PFFAAFCLPVFA 25 30 HPETLVKV AmpR PFFAAFCLPVFAHPETLVKV t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a

360 35 40 45 50 KDAEDQLGARVGYIELDLNS AmpR KDAEDQLGARVGYIELDLNS a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c

420 55 60 65 70 GKILESFRPEERFPMMSTFK AmpR GKILESFRPEERFPMMSTFK c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t

480 75 80 85 90 VLLCGAVLSRVDAGQEQLGR AmpR C G A T A C A C C G C G C C A T A A T A Amp-R VLLCGAVLSRVDAGQEQLGR a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c

540 95 100 105 110 RIHYSQNDLVEYSPVTEKHL AmpR RIHYSQNDLVEYSPVTEKHL c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a

600 115 120 125 130 TDGMTVRELCSAAITMSDNT AmpR TDGMTVRELCSAAITMSDNT t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g

660 135 140 145 150 AANLLLTTIGGPKELTAFLH AmpR AANLLLTTIGGPKELTAFLH t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t

720 155 160 165 170 NMGDHVTRLDRWEPELNEAI AmpR NMGDHVTRLDRWEPELNEAI c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a

780 175 180 185 190 PNDERDTTMPAAMATTLRKL AmpR PNDERDTTMPAAMATTLRKL a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g c a g c a a t g g c a a c a a c g t t g c g c a a a c t t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c g t c g t t a c c g t t g t t g c a a c g c g t t t g a

840 195 200 205 210 LTGELLTLASRQQLIDWMEA AmpR LTGELLTLASRQQLIDWMEA a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g

900 215 220 225 230 DKVAGPLLRSALPAGWFIAD AmpR DKVAGPLLRSALPAGWFIAD g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t

960 235 240 245 250 KSGAGERGSRGIIAALGPDG AmpR KSGAGERGSRGIIAALGPDG t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c

1020 255 260 265 270 KPSRIVVIYTTGSQATMDER AmpR KPSRIVVIYTTGSQATMDER t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c

1080 275 280 285 NRQIAEIGASLIKHW* AmpR NRQIAEIGASLIKHW* a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t

1140 a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t

1200 g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t

1260 ori c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c

1320 ori c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t

1380 ori t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t

1440 ori t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g

AlwNI 1500 ori t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c

1560 ori t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g

1620 ori g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a

1680 ori a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g

1740 ori G G G A A A C G C C T G pBR322ori-F g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c

1800 ori G T A T C T T T pBR322ori-F g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c

1860 ori c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a

1920 g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t

1980 t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c

2040 bom A G C G A G T C A G T G A G C G A G L4440 c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t

2100 bom A T G T T A G A C G A G A C T A C G G C pRS-marker t c t g t g c g g t a t t t c a c a c c g c a a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a a g a c a c g c c a t a a a g t g t g g c g t t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t

Tth111IPflFIBsaAIBstZ17I 2160 bom t a g t t a a g c c a g t a t a c a c t c c g c t a t c g c t a c g t g a c t g g g t c a t g g c t g c g c c c c g a c a t c a a t t c g g t c a t a t g t g a g g c g a t a g c g a t g c a c t g a c c c a g t a c c g a c g c g g g g c t g

2220 a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t

BsmBIEsp3I 2280 60 *LNEGDDGF rop C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t

PvuII 2340 55 50 45 40 RALCSRYLEDAHDHLSECID rop a a c g c g c g a g g c a g c t g c g g t a a a g c t c a t c a g c g t g g t c g t g a a g c g a t t c a c a g a t g t t t g c g c g c t c c g t c g a c g c c a t t t c g a g t a g t c g c a c c a g c a c t t c g c t a a g t g t c t a c a

2400 35 30 25 20 AQEDADLENLKELLTLTQSR rop c t g c c t g t t c a t c c g c g t c c a g c t c g t t g a g t t t c t c c a g a a g c g t t a a t g t c t g g c t t c g a c g g a c a a g t a g g c g c a g g t c g a g c a a c t c a a a g a g g t c t t c g c a a t t a c a g a c c g a a g

2460 15 10 5 1 IFRAMNLATKEQKTM rop t g a t a a a g c g g g c c a t g t t a a g g g c g g t t t t t t c c t g t t t g g t c a c t g a t g c c t c c g t g t a c t a t t t c g c c c g g t a c a a t t c c c g c c a a a a a a g g a c a a a c c a g t g a c t a c g g a g g c a c a

2520 a a g g g g g a t t t c t g t t c a t g g g g g t a a t g a t a c c g a t g a a a c g a g a g a g g a t g c t c a c g a t t c c c c c t a a a g a c a a g t a c c c c c a t t a c t a t g g c t a c t t t g c t c t c t c c t a c g a g t g c t

2580 t a c g g g t t a c t g a t g a t g a a c a t g c c c g g t t a c t g g a a c g t t g t g a g g g t a a a c a a c t g g a t g c c c a a t g a c t a c t a c t t g t a c g g g c c a a t g a c c t t g c a a c a c t c c c a t t t g t t g a c c

2640 c g g t a t g g a t g c g g c g g g a c c a g a g a a a a a t c a c t c a g g g t c a a t g c c a g c g c t t c g t t a g c c a t a c c t a c g c c g c c c t g g t c t c t t t t t a g t g a g t c c c a g t t a c g g t c g c g a a g c a a t

2700 a t a c a g a t g t a g g t g t t c c a c a g g g t a g c c a g c a g c a t c c t g c g a t g c a g a t c c g g a a c a t a t g t c t a c a t c c a c a a g g t g t c c c a t c g g t c g t c g t a g g a c g c t a c g t c t a g g c c t t g t

2760 t a a t g g t g c a g g g c g c t g a c t t c c g c g t t t c c a g a c t t t a c g a a a c a c g g a a a c c g a a g a a t t a c c a c g t c c c g c g a c t g a a g g c g c a a a g g t c t g a a a t g c t t t g t g c c t t t g g c t t c t

2820 c c a t t c a t g t t g t t g c t c a g g t c g c a g a c g t t t t g c a g c a g c a g t c g c t t c a c g t t c g c t g g t a a g t a c a a c a a c g a g t c c a g c g t c t g c a a a a c g t c g t c g t c a g c g a a g t g c a a g c g a

2880 c g c g t a t c g g t g a t t c a t t c t g c t a a c c a g t a a g g c a a c c c c g c c a g c c t a g c c g g g t c c g c g c a t a g c c a c t a a g t a a g a c g a t t g g t c a t t c c g t t g g g g c g g t c g g a t c g g c c c a g g

2940 t c a a c g a c a g g a g c a c g a t c a t g c g c a c c c g t g g c c a g g a c c c a a c g c t g c c c g a g a t g c a g t t g c t g t c c t c g t g c t a g t a c g c g t g g g c a c c g g t c c t g g g t t g c g a c g g g c t c t a c g

3000 g c c g c g t g c g g c t g c t g g a g a t g g c g g a c g c g a t g g a t a t g t t c t g c c a a g g g t t g g t t t c g g c g c a c g c c g a c g a c c t c t a c c g c c t g c g c t a c c t a t a c a a g a c g g t t c c c a a c c a a a

BsmI 3060 g c g c a t t c a c a g t t c t c c g c a a g a a t t g a t t g g c t c c a a t t c t t g g a g t g g t g a a t c c g t c g c g t a a g t g t c a a g a g g c g t t c t t a a c t a a c c g a g g t t a a g a a c c t c a c c a c t t a g g c a

3120 *TSTARSWAGRR t a g c g a g g t g c c g c c g g c t t c c a t t c a g g t c g a g g t g g c c c g g c t c c a t g c a c c g c g a c g a t c g c t c c a c g g c g g c c g a a g g t a a g t c c a g c t c c a c c g g g c c g a g g t a c g t g g c g c t g c

3180 LAPLCVLYLAAGVIWALGNW c a a c g c g g g g a g g c a g a c a a g g t a t a g g g c g g c g c c t a c a a t c c a t g c c a a c c c g t t c c a g t t g c g c c c c t c c g t c t g t t c c a t a t c c c g c c g c g g a t g t t a g g t a c g g t t g g g c a a g g t

3240 TSASAAYIATVILPGIISTL t g t g c t c g c c g a g g c g g c a t a a a t c g c c g t g a c g a t c a g c g g t c c a a t g a t c g a a g t t a g a c a c g a g c g g c t c c g c c g t a t t t a g c g g c a c t g c t a g t c g c c a g g t t a c t a g c t t c a a t c

3300 MVVIYLP STLAALSGQLQGQHDDDVQR g c t g g t a a g a g c c g c g a g c g a t c c t t g a a g c t g t c c c t g a t g g t c g t c a t c t a c c t g c c t c g a c c a t t c t c g g c g c t c g c t a g g a a c t t c g a c a g g g a c t a c c a g c a g t a g a t g g a c g g a

BstAPIBspMIBfuAI 3360 GQHGLQRGHPDAAGSEKNHN SLMAQLAPMGIGGSALLIMI g g a c a g c a t g g c c t g c a a c g c g g g c a t c c c g a t g c c g c c g g a a g c g a g a a g a a t c a t a a t c c t g t c g t a c c g g a c g t t g c g c c c g t a g g g c t a c g g c g g c c t t c g c t c t t c t t a g t a t t a

NruI 3420 GEGHPASRRERQQDVAQRVG PFAMWGRTAFALLVYGLADA g g g g a a g g c c a t c c a g c c t c g c g t c g c g a a c g c c a g c a a g a c g t a g c c c a g c g c g t c g g c c c c c t t c c g g t a g g t c g g a g c g c a g c g c t t g c g g t c g t t c t g c a t c g g g t c g c g c a g c c g

3480 RHAGDNGLLLAETFGGGTSD AMGAIIAQKEGFRKTAPGTV c g c c a t g c c g g c g a t a a t g g c c t g c t t c t c g c c g a a a c g t t t g g t g g c g g g a c c a g t g a c g c g g t a c g g c c g c t a t t a c c g g a c g a a g a g c g g c t t t g c a a a c c a c c g c c c t g g t c a c t g

3540 EGLSEGVQDSEYRKRQADHR FAQALAHLIGFVALSLGIMT g a a g g c t t g a g c g a g g g c g t g c a a g a t t c c g a a t a c c g c a a g c g a c a g g c c g a t c a t c g t c t t c c g a a c t c g c t c c c g c a c g t t c t a a g g c t t a t g g c g t t c g c t g t c c g g c t a g t a g c a

3600 RAPAKAVLAENDPERCRHLS ASWRFRDEGFIVWLAAPVQG c g c g c t c c a g c g a a a g c g g t c c t c g c c g a a a a t g a c c c a g a g c g c t g c c g g c a c c t g t c c g c g c g a g g t c g c t t t c g c c a g g a g c g g c t t t t a c t g g g t c t c g c g a c g g c c g t g g a c a g g

PshAI 3660 YELHDKEDSHKCGDDSHAPR VLQMIFFVTMLAAVITMGRA t a c g a g t t g c a t g a t a a a g a a g a c a g t c a t a a g t g c g g c g a c g a t a g t c a t g c c c c g c g c a t g c t c a a c g t a c t a t t t c t t c t g t c a g t a t t c a c g c c g c t g c t a t c a g t a c g g g g c g c g

SalI 3720 PPEGADWVEGSQGHRSTLSL WRFSSVPNFARLPMPRREGK c c a c c g g a a g g a g c t g a c t g g g t t g a a g g c t c t c a a g g g c a t c g g t c g a c g c t c t c c c t t g g t g g c c t t c c t c g a c t g a c c c a a c t t c c g a g a g t t c c c g t a g c c a g c t g c g a g a g g g a a

3780 MRLLH* HSEQMLFCGLLLNLGNLVAA a t g c g a c t c c t g c a t t a g g a a g c a g c c c a g t a g t a g g t t g a g g c c g t t g a g c a c c g c c g c t a c g c t g a g g a c g t a a t c c t t c g t c g g g t c a t c a t c c a a c t c c g g c a a c t c g t g g c g g c g

SphI 3840 ALFPAHLSIAGLLGGAVPGA c g c a a g g a a t g g t g c a t g c a a g g a g a t g g c g c c c a a c a g t c c c c c g g c c a c g g g g c c t g c g c g t t c c t t a c c a c g t a c g t t c c t c t a c c g c g g g t t g t c a g g g g g c c g g t g c c c c g g a c g

3900 VMGVGFCASM c a c c a t a c c c a c g c c g a a a c a a g c g c t c a t g a g c c c g a a g t g g c g a g c c c g a t c t t c c c c g t g g t a t g g g t g c g g c t t t g t t c g c g a g t a c t c g g g c t t c a c c g c t c g g g c t a g a a g g g g

SgrAI 3960 G G T G A T G T C G G C G A T A T A G G pBRrevBam a t c g g t g a t g t c g g c g a t a t a g g c g c c a g c a a c c g c a c c t g t g g c g c c g g t g a t g c c g g c t a g c c a c t a c a g c c g c t a t a t c c g c g g t c g t t g g c g t g g a c a c c g c g g c c a c t a c g g c c g

BglII 4020 T7 promoter T A A T A C G A C T C A C T T7 c a c g a t g c g t c c g g c g t a g a g g a t c g a g a t c t c g a t c c c g c g a a a t t a a t a c g a c t c a c t g t g c t a c g c a g g c c g c a t c t c c t a g c t c t a g a g c t a g g g c g c t t t a a t t a t g c t g a g t g a

NotIPacIEco53kISacI 4080 T7 promoter A T A G G G T7 a t a g g g a g a c c a c a a c g g t t t c c c t c t a g t g c c g g c t c c g g a g a g c t c t t t a a t t a a g c g t a t c c c t c t g g t g t t g c c a a a g g g a g a t c a c g g c c g a g g c c t c t c g a g a a a t t a a t t c g c

XbaISbfI 4140 RBS g c c g c c c t g c a g g a c t c g a g t t c t a g a a a t a a t t t t g t t t a a c t t t a a g a a g g a g a t a t a c g g c g g g a c g t c c t g a g c t c a a g a t c t t t a t t a a a a c a a a t t g a a a t t c t t c c t c t a t a t

BstXINdeI 4200 1 5 HHHHHH GSS 1 5 MSPILG 6xHis GST MKSSHHHHHHGSSMSPILG c a t a t g a a a t c t t c t c a c c a t c a c c a t c a c c a t g g t t c t t c t a t g t c c c c t a t a c t a g g t g t a t a c t t t a g a a g a g t g g t a g t g g t a g t g g t a c c a a g a a g a t a c a g g g g a t a t g a t c c a

4260 10 15 20 25 YWKIKGLVQPTRLLLEYLEE GST YWKIKGLVQPTRLLLEYLEE t a t t g g a a a a t t a a g g g c c t t g t g c a a c c c a c t c g a c t t c t t t t g g a a t a t c t t g a a g a a a t a a c c t t t t a a t t c c c g g a a c a c g t t g g g t g a g c t g a a g a a a a c c t t a t a g a a c t t c t t

4320 30 35 40 45 KYEEHLYERDEGDKWRNKKF GST KYEEHLYERDEGDKWRNKKF a a a t a t g a a g a g c a t t t g t a t g a g c g c g a t g a a g g t g a t a a a t g g c g a a a c a a a a a g t t t t t t a t a c t t c t c g t a a a c a t a c t c g c g c t a c t t c c a c t a t t t a c c g c t t t g t t t t t c a a a

4380 50 55 60 65 ELGLEFPNLPYYIDGDVKLT GST ELGLEFPNLPYYIDGDVKLT g a a t t g g g t t t g g a g t t t c c c a a t c t t c c t t a t t a t a t t g a t g g t g a t g t t a a a t t a a c a c t t a a c c c a a a c c t c a a a g g g t t a g a a g g a a t a a t a t a a c t a c c a c t a c a a t t t a a t t g t

4440 70 75 80 85 QSMAIIRYIADKHNMLGGCP GST QSMAIIRYIADKHNMLGGCP c a g t c t a t g g c c a t c a t a c g t t a t a t a g c t g a c a a g c a c a a c a t g t t g g g t g g t t g t c c a g t c a g a t a c c g g t a g t a t g c a a t a t a t c g a c t g t t c g t g t t g t a c a a c c c a c c a a c a g g t

4500 90 95 100 105 KERAEISMLEGAVLDIRYGV GST KERAEISMLEGAVLDIRYGV a a a g a g c g t g c a g a g a t t t c a a t g c t t g a a g g a g c g g t t t t g g a t a t t a g a t a c g g t g t t t t t c t c g c a c g t c t c t a a a g t t a c g a a c t t c c t c g c c a a a a c c t a t a a t c t a t g c c a c a a

4560 110 115 120 125 SRIAYSKDFETLKVDFLSKL GST SRIAYSKDFETLKVDFLSKL t c g a g a a t t g c a t a t a g t a a a g a c t t t g a a a c t c t c a a a g t t g a t t t t c t t a g c a a g c t a a g c t c t t a a c g t a t a t c a t t t c t g a a a c t t t g a g a g t t t c a a c t a a a a g a a t c g t t c g a t

BclI*SwaIBstBI 4620 130 135 140 145 PEMLKMFEDRLCHKTYLNGD GST PEMLKMFEDRLCHKTYLNGD c c t g a a a t g c t g a a a a t g t t c g a a g a t c g t t t a t g t c a t a a a a c a t a t t t a a a t g g t g a t g g a c t t t a c g a c t t t t a c a a g c t t c t a g c a a a t a c a g t a t t t t g t a t a a a t t t a c c a c t a

4680 150 155 160 165 HVTHPDFMLYDALDVVLYMD GST HVTHPDFMLYDALDVVLYMD c a t g t a a c c c a t c c t g a c t t c a t g t t g t a t g a c g c t c t t g a t g t t g t t t t a t a c a t g g a c g t a c a t t g g g t a g g a c t g a a g t a c a a c a t a c t g c g a g a a c t a c a a c a a a a t a t g t a c c t g

4740 170 175 180 185 PMCLDAFPKLVCFKKRIEAI GST PMCLDAFPKLVCFKKRIEAI c c a a t g t g c c t g g a t g c g t t c c c a a a a t t a g t t t g t t t t a a a a a a c g t a t t g a a g c t a t c g g t t a c a c g g a c c t a c g c a a g g g t t t t a a t c a a a c a a a a t t t t t t g c a t a a c t t c g a t a g

4800 190 195 200 205 PQIDKYLKSSKYIAWPLQGW GST PQIDKYLKSSKYIAWPLQGW c c a c a a a t t g a t a a g t a c t t g a a a t c c a g c a a g t a t a t a g c a t g g c c t t t g c a g g g c t g g g g t g t t t a a c t a t t c a t g a a c t t t a g g t c g t t c a t a t a t c g t a c c g g a a a c g t c c c g a c c

4860 210 215 QATFGGGDHPPK GIE 1 5 ENLYF GST TEV site QATFGGGDHPPKGIEENLYF c a a g c c a c g t t t g g t g g t g g c g a c c a t c c t c c a a a a g g g a t c g a g g a a a a c c t g t a c t t c g t t c g g t g c a a a c c a c c a c c g c t g g t a g g a g g t t t t c c c t a g c t c c t t t t g g a c a t g a a g

4920 QS (in frame with TEV site) NAHDVLETIFVRKVGAFV TEV site QSNAHDVLETIFVRKVGAFV c a a t c c a a t g c a c a c g a t g t c c t g g a a a c c a t c t t c g t g a g a a a a g t c g g c g c t t t t g t g g t t a g g t t a c g t g t g c t a c a g g a c c t t t g g t a g a a g c a c t c t t t t c a g c c g c g a a a a c a c

4980 (in frame with TEV site) NKPINQVTLTSLDIPFAMFA NKPINQVTLTSLDIPFAMFA a a c a a g c c c a t t a a t c a g g t c a c a c t c a c c t c t c t g g a c a t c c c t t t c g c t a t g t t t g c a t t g t t c g g g t a a t t a g t c c a g t g t g a g t g g a g a g a c c t g t a g g g a a a g c g a t a c a a a c g t

5040 (in frame with TEV site) PKNLELEDTDPMVNPPDSPE PKNLELEDTDPMVNPPDSPE c c a a a g a a c c t c g a g c t g g a a g a c a c t g a t c c c a t g g t g a a t c c t c c a g a c t c a c c c g a g g g t t t c t t g g a g c t c g a c c t t c t g t g a c t a g g g t a c c a c t t a g g a g g t c t g a g t g g g c t c

5100 (in frame with TEV site) TESPLQGSLHSDGSSGGSSG TESPLQGSLHSDGSSGGSSG a c a g a a a g t c c t c t c c a g g g c a g t c t g c a c a g c g a t g g a a g c t c c g g t g g c t c t t c a g g c t g t c t t t c a g g a g a g g t c c c g t c a g a c g t g t c g c t a c c t t c g a g g c c a c c g a g a a g t c c g

5160 (in frame with TEV site) NTHDDFVMIDFKPAFSKDDI NTHDDFVMIDFKPAFSKDDI a a c a c a c a t g a c g a t t t c g t c a t g a t t g a c t t c a a a c c c g c t t t t t c c a a g g a c g a t a t c t t g t g t g t a c t g c t a a a g c a g t a c t a a c t g a a g t t t g g g c g a a a a a g g t t c c t g c t a t a g

5220 (in frame with TEV site) LPMDLGTFYREFQNPPQLSS LPMDLGTFYREFQNPPQLSS c t c c c t a t g g a t c t g g g a a c c t t c t a c a g g g a g t t t c a g a a t c c c c c t c a g c t c a g t a g c g a g g g a t a c c t a g a c c c t t g g a a g a t g t c c c t c a a a g t c t t a g g g g g a g t c g a g t c a t c g

5280 (in frame with TEV site) LSIDIGAQSMAEDLDSLPEK LSIDIGAQSMAEDLDSLPEK c t g a g c a t t g a c a t c g g t g c c c a g t c c a t g g c t g a g g a c c t c g a t t c t c t g c c t g a a a a a g a c t c g t a a c t g t a g c c a c g g g t c a g g t a c c g a c t c c t g g a g c t a a g a g a c g g a c t t t t t

5340 (in frame with TEV site) LAVHEKNVREFDAFVETLQ* LAVHEKNVREFDAFVETLQ* c t g g c t g t g c a c g a g a a g a a c g t c a g a g a a t t c g a c g c a t t t g t g g a g a c c c t g c a g t a a g a c c g a c a c g t g c t c t t c t t g c a g t c t c t t a a g c t g c g t a a a c a c c t c t g g g a c g t c a t t

FseIAcc65IAsiSIAscISexAI*CsiIDraIIIBamHI 5400 t a a c a t t g g a a g t g g a t a a c g g a t c c g c g a t c g c g g c g c g c c a c c t g g t g g c c g g c c g g t a t t g t a a c c t t c a c c t a t t g c c t a g g c g c t a g c g c c g c g c g g t g g a c c a c c g g c c g g c c a

KpnIMluI 5460 a c c a c g c g t g c g c g c t g a t c c g g c t g c t a a c a a a g c c c g a a a g g a a g c t g a g t t g g c t g c t g g t g c g c a c g c g c g a c t a g g c c g a c g a t t g t t t c g g g c t t t c c t t c g a c t c a a c c g a c g

BlpI 5520 T7 terminator G G C G A C T C G T T A T T G A T C G T7 Term t g c c a c c g c t g a g c a a t a a c t a g c a t a a c c c c t t g g g g c c t c t a a a c g g g t c t t g a g g g g a c g g t g g c g a c t c g t t a t t g a t c g t a t t g g g g a a c c c c g g a g a t t t g c c c a g a a c t c c c c

5580 T7 terminator t t t t t t g c t g a a a g g a g g a a c t a t a t c c g g a t a t c c a c a g g a c g g g t g t g g t c g c c a t g a a a a a a a c g a c t t t c c t c c t t g a t a t a g g c c t a t a g g t g t c c t g c c c a c a c c a g c g g t a c t

5640 t c g c g t a g t c g a t a g t g g c t c c a a g t a g c g a a g c g a g c a g g a c t g g g c g g c g g c c a a a g c a g c g c a t c a g c t a t c a c c g a g g t t c a t c g c t t c g c t c g t c c t g a c c c g c c g c c g g t t t c g

NheI 5700 g g t c g g a c a g t g c t c c g a g a a c g g g t g c g c a t a g a a a t t g c a t c a a c g c a t a t a g c g c t a c c a g c c t g t c a c g a g g c t c t t g c c c a c g c g t a t c t t t a a c g t a g t t g c g t a t a t c g c g a t

BmtI 5760 g c a g c a c g c c a t a g t g a c t g g c g a t g c t g t c g g a a t g g a c g a t a t c c c g c a a g a g g c c c g c g t c g t g c g g t a t c a c t g a c c g c t a c g a c a g c c t t a c c t g c t a t a g g g c g t t c t c c g g g c

5820 g c a g t a c c g g c a t a a c c a a g c c t a t g c c t a c a g c a t c c a g g g t g a c g g t g c c g a g g a t g a c g t c a t g g c c g t a t t g g t t c g g a t a c g g a t g t c g t a g g t c c c a c t g c c a c g g c t c c t a c t

5880 c g a t g a g c g c a t t g t t a g a t t t c a t a c a c g g t g c c t g a c t g c g t t a g c a a t t t a a c t g t g g c t a c t c g c g t a a c a a t c t a a a g t a t g t g c c a c g g a c t g a c g c a a t c g t t a a a t t g a c a c

BspDIClaIHindIII 3ʹ 5ʹ 5930 -10 -35 tet promoter a t a a a c t a c c g c a t t a a a g c t t a t c g a t g a t a a g c t g t c a a a c a t g a g a a t a t t t g a t g g c g t a a t t t c g a a t a g c t a c t a t t c g a c a g t t t g t a c t c t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.