Addgene pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1 Sequencing Result


Map View

NotI (7879) hU6-F (7842 .. 7862) LKO.1 5' (7672 .. 7691) NcoI (7598) HindIII (7495) PaeR7I - PspXI - XhoI (7483) SphI (7458) SV40 NLS BstZ17I (7102) SalI (7002) EcoRV (6974) BmgBI (6689) BbvCI (6556) BspDI * - ClaI * (6397) XcmI (6242) PfoI * (6202) BsmBI - Esp3I (6179) PpuMI (5779) ApaI * (4662) PspOMI * (4658) StuI (4363) M13 fwd M13 Forward (136 .. 153) M13/pUC Forward (145 .. 167) F1ori-R (381 .. 400) F1ori-F (591 .. 612) PsiI (652) Amp-R (1098 .. 1117) BpmI (1598) pBR322ori-F (2400 .. 2419) PciI (2555) CAP binding site M13/pUC Reverse (2866 .. 2888) lac operator M13 Reverse (2885 .. 2901) M13 rev XbaI (3064) NheI (3074) BmtI (3078) MfeI (3175) SV40 NLS Eco53kI (3892) SacI (3894) BspEI (3903) pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1 8006 bp

Sequence View

60 g a t g g a g t t g g c c a c t c c c t c t c t g c g c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a c t a c c t c a a c c g g t g a g g g a g a g a c g c g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t

120 g g t c g c c c g a c g c c c g g g c t t t g c c c g g g c g g c c t c a g t g a g c g a g c g a g c g c g c a g c c t c c a g c g g g c t g c g g g c c c g a a a c g g g c c c g c c g g a g t c a c t c g c t c g c t c g c g c g t c g g a

180 M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward t a a t t a a c c t a a t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t a t t a a t t g g a t t a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a

240 t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t

300 f1 ori g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g g a c g c g c c c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c c t g c g c g g

360 f1 ori c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a

420 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g

480 f1 ori c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a

540 f1 ori a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g

600 f1 ori G T G G A C T C T T F1ori-F c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a

PsiI 660 f1 ori G T T C C A A A C T G G F1ori-F g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a

720 f1 ori t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t

780 f1 ori AmpR promoter t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t

840 AmpR promoter a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t

900 1 5 signal sequence MSIQHFR AmpR promoter AmpR MSIQHFR c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a

960 10 15 20 signal sequence VALIPFFAAFCLPVFA 25 HPET AmpR VALIPFFAAFCLPVFAHPET g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c

1020 30 35 40 45 LVKVKDAEDQLGARVGYIEL AmpR LVKVKDAEDQLGARVGYIEL c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c

1080 50 55 60 65 DLNSGKILESFRPEERFPMM AmpR DLNSGKILESFRPEERFPMM g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c

1140 70 75 80 85 STFKVLLCGAVLSRIDAGQE AmpR C G A T A C A C C G C G C C A T A A T A Amp-R STFKVLLCGAVLSRIDAGQE a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c

1200 90 95 100 105 QLGRRIHYSQNDLVEYSPVT AmpR QLGRRIHYSQNDLVEYSPVT c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t

1260 110 115 120 125 EKHLTDGMTVRELCSAAITM AmpR EKHLTDGMTVRELCSAAITM g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c

1320 130 135 140 145 SDNTAANLLLTTIGGPKELT AmpR SDNTAANLLLTTIGGPKELT a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g

1380 150 155 160 165 AFLHNMGDHVTRLDRWEPEL AmpR AFLHNMGDHVTRLDRWEPEL g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c

1440 170 175 180 185 NEAIPNDERDTTMPVAMATT AmpR NEAIPNDERDTTMPVAMATT a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c

1500 190 195 200 205 LRKLLTGELLTLASRQQLID AmpR LRKLLTGELLTLASRQQLID t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g

1560 210 215 220 225 WMEADKVAGPLLRSALPAGW AmpR WMEADKVAGPLLRSALPAGW t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c

BpmI 1620 230 235 240 245 FIADKSGAGERGSRGIIAAL AmpR FIADKSGAGERGSRGIIAAL t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c

1680 250 255 260 265 GPDGKPSRIVVIYTTGSQAT AmpR GPDGKPSRIVVIYTTGSQAT g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a

1740 270 275 280 285 MDERNRQIAEIGASLIKHW* AmpR MDERNRQIAEIGASLIKHW* a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t

1800 c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a

1860 a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c

1920 ori t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a

1980 ori t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a

2040 ori t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c

2100 ori c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a

2160 ori g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g

2220 ori g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c

2280 ori t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t

2340 ori c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c

2400 ori G pBR322ori-F g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c

2460 ori G G A A A C G C C T G G T A T C T T T pBR322ori-F g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t

2520 ori t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a

PciI 2580 t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a

2640 g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t

2700 a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c

2760 c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c

2820 CAP binding site a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c

2880 -35 -10 lac promoter lac operator A G C G G A T A A C A A T T T M13/pUC Reverse g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a

2940 M13 rev AAV2 ITR C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a g a t t t a a t t a a g g c t g c g c g c t c g c t g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t c t a a a t t a a t t c c g a c g c g c g a g c g a

3000 AAV2 ITR c g c t c a c t g a g g c c g c c c g g g c a a a g c c c g g g c g t c g g g c g a c c t t t g g t c g c c c g g c c t g c g a g t g a c t c c g g c g g g c c c g t t t c g g g c c c g c a g c c c g c t g g a a a c c a g c g g g c c g g a

3060 AAV2 ITR c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a a c t c c a t c a c t a g g g g t t c c t g c g g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t t g a g g t a g t g a t c c c c a a g g a c g c

BmtINheIXbaI 3120 *FESALDQFSHSNAGTGTL g c c t c t a g a a t t c g c t a g c t a g g t c t t g a a a g g a g t g g g a a t t g g c t c c g g t g c c c g t c a c g g a g a t c t t a a g c g a t c g a t c c a g a a c t t t c c t c a c c c t t a a c c g a g g c c a c g g g c a g t

MfeI 3180 EF-1 α core promoter PCLACRGCDGLLQPSPDAIS g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t t g c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a a c

3240 EF-1 α core promoter GTGLSPPAPYVPFTIDHVPE a t c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g c t t a g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c g a

3300 EF-1 α core promoter AKKGLTPSFRIYTCYDGHVN c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g t g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g c a

3360 EF-1 α core promoter MKRT KKAVPKGGSCLVPAVMFRVA t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g a c c g g t g c c a c c a t g a a a c g g a c a g a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c t g g c c a c g g t g g t a c t t t g c c t g t c

3420 130 PKKKRKV (in frame with SV40 NLS) SEVEF SV40 NLS ADGSEFESPKKKRKVSEVEF SPLSNSDGFFFRFTESTSNE c c g a c g g a a g c g a g t t c g a g t c a c c a a a g a a g a a g c g g a a a g t c t c t g a g g t g g a g t t t t g g c t g c c t t c g c t c a a g c t c a g t g g t t t c t t c t t c g c c t t t c a g a g a c t c c a c c t c a a a a

3480 (in frame with SV40 NLS) SHEYWMRHALTLAKRARDER SHEYWMRHALTLAKRARDER WSYQILCARVRALLARSSLS c c c a c g a g t a c t g g a t g a g a c a t g c c c t g a c c c t g g c c a a g a g g g c a c g g g a t g a g a g g g g g g t g c t c a t g a c c t a c t c t g t a c g g g a c t g g g a c c g g t t c t c c c g t g c c c t a c t c t c c c

3540 (in frame with SV40 NLS) EVPVGAVLVLNNRVIGEGWN EVPVGAVLVLNNRVIGEGWN TGTPATSTSFLLTIPSPQFL a g g t g c c t g t g g g a g c c g t g c t g g t g c t g a a c a a t a g a g t g a t c g g c g a g g g c t g g a a c a t c c a c g g a c a c c c t c g g c a c g a c c a c g a c t t g t t a t c t c a c t a g c c g c t c c c g a c c t t g t

3600 (in frame with SV40 NLS) RAIGLHDPTAHAEIMALRQG RAIGLHDPTAHAEIMALRQG AMPRCSGVAWASIIARLCPP g a g c c a t c g g c c t g c a c g a c c c a a c a g c c c a t g c c g a a a t t a t g g c c c t g a g a c a g g g c g c t c g g t a g c c g g a c g t g c t g g g t t g t c g g g t a c g g c t t t a a t a c c g g g a c t c t g t c c c g c

3660 (in frame with SV40 NLS) GLVMQNYRLIDATLYVTFEP GLVMQNYRLIDATLYVTFEP RTM g c c t g g t c a t g c a g a a c t a c a g a c t g a t t g a c g c c a c c c t g t a c g t g a c a t t c g a g c c t t c g g a c c a g t a c g t c t t g a t g t c t g a c t a a c t g c g g t g g g a c a t g c a c t g t a a g c t c g g a a

3720 (in frame with SV40 NLS) CVMCAGAMIHSRIGRVVFGV CVMCAGAMIHSRIGRVVFGV g c g t g a t g t g c g c c g g c g c c a t g a t c c a c t c t a g g a t c g g c c g c g t g g t g t t t g g c g t g a c g c a c t a c a c g c g g c c g c g g t a c t a g g t g a g a t c c t a g c c g g c g c a c c a c a a a c c g c a c t

3780 (in frame with SV40 NLS) RNSKRGAAGSLMNVLNYPGM RNSKRGAAGSLMNVLNYPGM g g a a c t c a a a a a g a g g c g c c g c a g g c t c c c t g a t g a a c g t g c t g a a c t a c c c c g g c a t g a c c t t g a g t t t t t c t c c g c g g c g t c c g a g g g a c t a c t t g c a c g a c t t g a t g g g g c c g t a c t

3840 (in frame with SV40 NLS) NHRVEITEGILADECAALLC NHRVEITEGILADECAALLC a t c a c c g c g t c g a a a t t a c c g a g g g a a t c c t g g c a g a t g a a t g t g c c g c c c t g c t g t g c g t a g t g g c g c a g c t t t a a t g g c t c c c t t a g g a c c g t c t a c t t a c a c g g c g g g a c g a c a c g c

Eco53kISacI 3900 (in frame with SV40 NLS) DFYRMPRQVFNAQKKAQSSI DFYRMPRQVFNAQKKAQSSI a t t t c t a t c g g a t g c c t a g a c a g g t g t t c a a t g c t c a g a a g a a g g c c c a g a g c t c c a t c a t a a a g a t a g c c t a c g g a t c t g t c c a c a a g t t a c g a g t c t t c t t c c g g g t c t c g a g g t a g t

BspEI 3960 (in frame with SV40 NLS) NSGGSSGGSSGSETPGTSES NSGGSSGGSSGSETPGTSES a c t c c g g a g g a t c t a g c g g a g g c t c c t c t g g c t c t g a g a c a c c t g g c a c a a g c g a g a g c g t g a g g c c t c c t a g a t c g c c t c c g a g g a g a c c g a g a c t c t g t g g a c c g t g t t c g c t c t c g c

4020 (in frame with SV40 NLS) ATPESSGGSSGGSMQENQQK ATPESSGGSSGGSMQENQQK c a a c a c c t g a a a g c a g c g g g g g c a g c a g c g g g g g g t c a a t g c a g g a g a a c c a g c a g a a g c g t t g t g g a c t t t c g t c g c c c c c g t c g t c g c c c c c c a g t t a c g t c c t c t t g g t c g t c t t c g

4080 (in frame with SV40 NLS) QNYILGLAIGITSVGYGLID QNYILGLAIGITSVGYGLID a a a a t t a c a t c c t g g g c c t g g c c a t c g g c a t c a c c a g c g t g g g c t a t g g c c t g a t c g a c a t t t t a a t g t a g g a c c c g g a c c g g t a g c c g t a g t g g t c g c a c c c g a t a c c g g a c t a g c t g t

4140 (in frame with SV40 NLS) SKTREVIDAGVRLFPEADSE SKTREVIDAGVRLFPEADSE g c a a g a c c a g a g a a g t g a t t g a c g c c g g c g t g c g g c t a t t c c c a g a g g c c g a c t c t g a a a c g t t c t g g t c t c t t c a c t a a c t g c g g c c g c a c g c c g a t a a g g g t c t c c g g c t g a g a c t t t

4200 (in frame with SV40 NLS) NNSNRRSKRGARRLKRRRIH NNSNRRSKRGARRLKRRRIH a c a a c a g c a a t a g a a g a t c t a a g c g g g g c g c c c g g a g a c t g a a a a g a c g a a g a a t c c a c a t g t t g t c g t t a t c t t c t a g a t t c g c c c c g c g g g c c t c t g a c t t t t c t g c t t c t t a g g t g t

4260 (in frame with SV40 NLS) RLNRVKDLLADYQMIDLNNV RLNRVKDLLADYQMIDLNNV *VVH g a c t g a a c a g a g t g a a a g a c c t c c t g g c t g a c t a c c a g a t g a t c g a c t t a a a c a a c g t g c c t g a c t t g t c t c a c t t t c t g g a g g a c c g a c t g a t g g t c t a c t a g c t g a a t t t g t t g c a c g

4320 (in frame with SV40 NLS) PKSTDPYTIRVKGLREPLTK PKSTDPYTIRVKGLREPLTK GLRGVGVGDPHLSQPFRQGL c c a a g t c t a c c g a c c c c t a c a c c a t c c g g g t g a a g g g a c t g c g g g a a c c t c t g a c c a a g g g g t t c a g a t g g c t g g g g a t g t g g t a g g c c c a c t t c c c t g a c g c c c t t g g a g a c t g g t t c c

StuI 4380 (in frame with SV40 NLS) EEFAIALLHIAKRRGLHNIS EEFAIALLHIAKRRGLHNIS FLKGDSQQMDGLSSAQVVDG a a g a g t t t g c c a t c g c t c t g c t g c a t a t c g c c a a g a g a a g a g g c c t g c a c a a c a t c t c c g t t c t c a a a c g g t a g c g a g a c g a c g t a t a g c g g t t c t c t t c t c c g g a c g t g t t g t a g a g g c

4440 (in frame with SV40 NLS) VSMGDEEQDNELSTKQQLQK VSMGDEEQDNELSTKQQLQK HAHAILFLVVLQGGLLLQLL t g a g c a t g g g c g a t g a g g a a c a g g a c a a c g a g c t g t c c a c c a a g c a g c a g c t g c a g a a g a a c t c g t a c c c g c t a c t c c t t g t c c t g t t g c t c g a c a g g t g g t t c g t c g t c g a c g t c t t c t

4500 (in frame with SV40 NLS) NAQQLQDKYVCELQLERLTN NAQQLQDKYVCELQLERLTN VSLLQLVFVHALQLQFSQGV a c g c t c a g c a g c t g c a g g a c a a a t a c g t g t g c g a g c t g c a a c t g g a a a g a c t g a c c a a c a t g c g a g t c g t c g a c g t c c t g t t t a t g c a c a c g c t c g a c g t t g a c c t t t c t g a c t g g t t g t

4560 (in frame with SV40 NLS) INKVRGEKNRFKTEDFVKEV INKVRGEKNRFKTEDFVKEV DVLDSALLVPELGFIEHLFH t c a a c a a g g t c a g a g g c g a g a a g a a c c g g t t c a a g a c c g a a g a t t t c g t g a a g g a a g t g a a g t t g t t c c a g t c t c c g c t c t t c t t g g c c a a g t t c t g g c t t c t a a a g c a c t t c c t t c a c t

4620 (in frame with SV40 NLS) KQLCETQRQYHNIDDQFIQQ KQLCETQRQYHNIDDQFIQQ LLQALGLPLVVVDVILEDLL a g c a g c t g t g c g a g a c c c a g c g g c a g t a c c a c a a c a t c g a c g a t c a g t t c a t c c a g c a g t t c g t c g a c a c g c t c t g g g t c g c c g t c a t g g t g t t g t a g c t g c t a g t c a a g t a g g t c g t c a

PspOMI*ApaI* 4680 (in frame with SV40 NLS) YIDLVSTRREYFEGPGNGSP YIDLVSTRREYFEGPGNGSP VDVQHAGPSFVELARAVARW a c a t c g a c c t g g t g a g c a c c c g g a g a g a a t a c t t c g a g g g c c c t g g c a a c g g c t c t c c a t t g t a g c t g g a c c a c t c g t g g g c c t c t c t t a t g a a g c t c c c g g g a c c g t t g c c g a g a g g t a

4740 (in frame with SV40 NLS) YGWDGDLLKWYEKLMGRCTY YGWDGDLLKWYEKLMGRCTY IAPISIQQLPILLQHASAGV a t g g c t g g g a t g g a g a t c t g c t g a a g t g g t a t g a g a a g c t g a t g g g c a g a t g c a c c t a c t t a c c g a c c c t a c c t c t a g a c g a c t t c a c c a t a c t c t t c g a c t a c c c g t c t a c g t g g a t g a

4800 (in frame with SV40 NLS) FPEELRSVKYAYSADLFNAL FPEELRSVKYAYSADLFNAL ERLLQSAHLVGVAGIQKVGQ t c c c t g a g g a g c t g a g a a g c g t g a a g t a c g c c t a c a g c g c c g a t c t g t t t a a c g c c c t g a a g g g a c t c c t c g a c t c t t c g c a c t t c a t g c g g a t g t c g c g g c t a g a c a a a t t g c g g g a c t

4860 (in frame with SV40 NLS) NDLNNLVVTRDDNPKLEYYE NDLNNLVVTRDDNPKLEYYE VIQVVQNHGAVVVRLQFVVL a c g a t c t g a a c a a c c t g g t t g t g a c c c g c g a c g a c a a c c c t a a g c t g g a a t a c t a c g a g a t g c t a g a c t t g t t g g a c c a a c a c t g g g c g c t g c t g t t g g g a t t c g a c c t t a t g a t g c t c t

4920 (in frame with SV40 NLS) KYHIIENVFKQKKNPTLKQI KYHIIENVFKQKKNPTLKQI FVVNDLVHELLLFIWSQLLD a a t a c c a c a t t a t c g a g a a c g t g t t c a a g c a g a a g a a a a a t c c a a c t c t g a a g c a a a t c g t t a t g g t g t a a t a g c t c t t g c a c a a g t t c g t c t t c t t t t t a g g t t g a g a c t t c g t t t a g c

4980 (in frame with SV40 NLS) AKEIGVQDYDIRGYRITKSG AKEIGVQDYDIRGYRITKSG GFLDAHLIVVDSSVSNGLGT c c a a a g a g a t c g g c g t g c a g g a t t a c g a c a t c a g a g g a t a c a g a a t t a c c a a g t c c g g t a g g t t t c t c t a g c c g c a c g t c c t a a t g c t g t a g t c t c c t a t g t c t t a a t g g t t c a g g c c a t

5040 (in frame with SV40 NLS) KPQFTSFKLYHDLKNIFEQA KPQFTSFKLYHDLKNIFEQA LRLEGAEFEVVVQFVDKFLG a g c c t c a g t t c a c c a g c t t c a a a c t c t a c c a c g a c c t g a a a a a c a t c t t t g a a c a g g c c a t c g g a g t c a a g t g g t c g a a g t t t g a g a t g g t g c t g g a c t t t t t g t a g a a a c t t g t c c g g t

5100 (in frame with SV40 NLS) KYLEDVEMLDEIAKILTIYQ KYLEDVEMLDEIAKILTIYQ FVQFIHLHQILYSFDQCDVL a a t a c c t g g a a g a t g t g g a g a t g c t g g a t g a g a t a g c t a a a a t c c t g a c a a t c t a c c a a g t t a t g g a c c t t c t a c a c c t c t a c g a c c t a c t c t a t c g a t t t t a g g a c t g t t a g a t g g t t c

5160 (in frame with SV40 NLS) DEISIKKALDQLPELLTESE DEISIKKALDQLPELLTESE VLDADLFGQVLQRLQQGLAF a c g a g a t c a g c a t c a a g a a a g c c c t g g a c c a g c t g c c t g a g c t g c t g a c c g a g a g c g a a a t g c t c t a g t c g t a g t t c t t t c g g g a c c t g g t c g a c g g a c t c g a c g a c t g g c t c t c g c t t t

5220 (in frame with SV40 NLS) KSQIAQLTGYTGTHRLSLKC KSQIAQLTGYTGTHRLSLKC FALDSLQGAVGTCMSQRQLA a a a g c c a g a t c g c t c a g c t g a c c g g c t a c a c c g g t a c a c a t a g a c t g t c t c t g a a g t g c a t t t c g g t c t a g c g a g t c g a c t g g c c g a t g t g g c c a t g t g t a t c t g a c a g a g a c t t c a c g t

5280 (in frame with SV40 NLS) IHIVIDELWESPENQMEIFT IHIVIDELWESPENQMEIFT DVDHDVLQPLAGFVLHFDEG t c c a c a t c g t g a t c g a c g a g c t g t g g g a g a g c c c c g a a a a c c a g a t g g a a a t c t t c a c c a a g g t g t a g c a c t a g c t g c t c g a c a c c c t c t c g g g g c t t t t g g t c t a c c t t t a g a a g t g g t

5340 (in frame with SV40 NLS) RLNLKPKKVEMSEIDSIPTT RLNLKPKKVEMSEIDSIPTT SQVELWLLHFHALDVADRGC g a c t g a a c c t c a a g c c a a a g a a g g t g g a a a t g a g c g a g a t c g a c a g c a t c c c t a c c a c a c c t g a c t t g g a g t t c g g t t t c t t c c a c c t t t a c t c g c t c t a g c t g t c g t a g g g a t g g t g t g

5400 (in frame with SV40 NLS) LVDEFILSPVVKRAFIQSIK LVDEFILSPVVKRAFIQSIK QHILEDQARHHLPGEDLGDL t g g t g g a t g a g t t c a t c c t g a g c c c t g t g g t g a a g c g g g c c t t c a t c c a g t c c a t c a a g g a c c a c c t a c t c a a g t a g g a c t c g g g a c a c c a c t t c g c c c g g a a g t a g g t c a g g t a g t t c c

5460 (in frame with SV40 NLS) VINAVINRFGLPEDIIIELA VINAVINRFGLPEDIIIELA HDVSHDVSEAQGLVDNDLQG t g a t c a a c g c t g t g a t c a a c a g a t t c g g c c t g c c c g a g g a c a t c a t t a t c g a g c t g g c c a a c t a g t t g c g a c a c t a g t t g t c t a a g c c g g a c g g g c t c c t g t a g t a a t a g c t c g a c c g g t

5520 (in frame with SV40 NLS) REKNSKDRRKFINKLQKQNE REKNSKDRRKFINKLQKQNE SLLVALVSPLEDVLQLFLIL g a g a g a a g a a c a g c a a g g a c a g a a g g a a g t t c a t c a a c a a g c t g c a g a a a c a g a a t g a g g c t c t c t t c t t g t c g t t c c t g t c t t c c t t c a a g t a g t t g t t c g a c g t c t t t g t c t t a c t c c

5580 (in frame with SV40 NLS) ATRKKIEQLLAKYGNTNAKY ATRKKIEQLLAKYGNTNAKY GGPFFDLLQQGLVAIGIGLV c c a c c c g g a a a a a a a t c g a g c a g c t g c t g g c c a a g t a c g g c a a t a c c a a t g c c a a g t a c a g g t g g g c c t t t t t t t a g c t c g t c g a c g a c c g g t t c a t g c c g t t a t g g t t a c g g t t c a t g t

5640 (in frame with SV40 NLS) MIEKIKLHDMQEGKCLYSLE MIEKIKLHDMQEGKCLYSLE HDFLDLQM t g a t c g a a a a g a t c a a g c t g c a t g a c a t g c a g g a g g g c a a g t g t c t g t a c a g c c t g g a a g a c t a g c t t t t c t a g t t c g a c g t a c t g t a c g t c c t c c c g t t c a c a g a c a t g t c g g a c c t t c

5700 (in frame with SV40 NLS) AIPLEDLLSNPTHYEVDHII AIPLEDLLSNPTHYEVDHII c t a t c c c c c t g g a a g a c c t g c t g t c t a a t c c t a c a c a c t a c g a g g t g g a c c a c a t c a t c c g a t a g g g g g a c c t t c t g g a c g a c a g a t t a g g a t g t g t g a t g c t c c a c c t g g t g t a g t a g g

5760 (in frame with SV40 NLS) PRSVSFDNSLNNKVLVKQSE PRSVSFDNSLNNKVLVKQSE c t a g a a g c g t g t c c t t c g a c a a c a g c c t g a a c a a c a a g g t t c t g g t g a a g c a a a g c g a g a g a t c t t c g c a c a g g a a g c t g t t g t c g g a c t t g t t g t t c c a a g a c c a c t t c g t t t c g c t c t

PpuMI 5820 (in frame with SV40 NLS) NSKKGNRTPYQYLSSNESKI NSKKGNRTPYQYLSSNESKI a c a g c a a g a a g g g c a a t a g g a c c c c t t a c c a g t a c c t g a g c a g c a a c g a g t c c a a g a t c t t g t c g t t c t t c c c g t t a t c c t g g g g a a t g g t c a t g g a c t c g t c g t t g c t c a g g t t c t a g a

5880 (in frame with SV40 NLS) SYNQFKQHILNLSKAKDRIS SYNQFKQHILNLSKAKDRIS c t t a c a a c c a g t t c a a g c a g c a c a t t c t g a a c c t g t c t a a g g c c a a a g a t a g a a t c a g c a g a a t g t t g g t c a a g t t c g t c g t g t a a g a c t t g g a c a g a t t c c g g t t t c t a t c t t a g t c g t

5940 (in frame with SV40 NLS) KKKRDMLLEERDINKFEVQK KKKRDMLLEERDINKFEVQK a g a a g a a a c g a g a t a t g c t g c t g g a a g a a c g g g a c a t c a a c a a a t t c g a g g t g c a g a a g g t c t t c t t t g c t c t a t a c g a c g a c c t t c t t g c c c t g t a g t t g t t t a a g c t c c a c g t c t t c c

6000 (in frame with SV40 NLS) EFINRNLVDTRYATRELSNL EFINRNLVDTRYATRELSNL a a t t c a t c a a c a g a a a c c t t g t g g a c a c c c g g t a c g c c a c t c g g g a g c t g a g c a a c c t g c t t a a g t a g t t g t c t t t g g a a c a c c t g t g g g c c a t g c g g t g a g c c c t c g a c t c g t t g g a c g

6060 (in frame with SV40 NLS) LKTYFSTHDYAVKVKTINGG LKTYFSTHDYAVKVKTINGG t g a a g a c c t a c t t c a g c a c a c a c g a c t a c g c c g t g a a a g t g a a g a c c a t c a a c g g c g g c t a c t t c t g g a t g a a g t c g t g t g t g c t g a t g c g g c a c t t t c a c t t c t g g t a g t t g c c g c c g a

6120 (in frame with SV40 NLS) FTNHLRKVWDFKKHRNHGYK FTNHLRKVWDFKKHRNHGYK t c a c a a a c c a c c t g a g g a a g g t g t g g g a c t t c a a g a a g c a c c g g a a c c a c g g c t a c a a g c a g t g t t t g g t g g a c t c c t t c c a c a c c c t g a a g t t c t t c g t g g c c t t g g t g c c g a t g t t c g

BsmBIEsp3I 6180 (in frame with SV40 NLS) HHAEDALVIANADFLFKTHK HHAEDALVIANADFLFKTHK a c c a c g c c g a g g a t g c c c t g g t c a t c g c c a a c g c c g a c t t t c t g t t c a a a a c c c a c a a g g t g g t g c g g c t c c t a c g g g a c c a g t a g c g g t t g c g g c t g a a a g a c a a g t t t t g g g t g t t c c

PfoI* 6240 (in frame with SV40 NLS) ALRRTDKILEQPGLEVNDTT ALRRTDKILEQPGLEVNDTT c c c t g a g a c g g a c a g a c a a g a t c c t g g a a c a g c c t g g a c t g g a a g t c a a c g a c a c c a c c g g g g a c t c t g c c t g t c t g t t c t a g g a c c t t g t c g g a c c t g a c c t t c a g t t g c t g t g g t g g c

XcmI 6300 (in frame with SV40 NLS) VKVDTEEKYQELFETPKQVK VKVDTEEKYQELFETPKQVK t g a a g g t g g a c a c a g a g g a g a a g t a c c a g g a g t t a t t c g a g a c a c c g a a a c a a g t g a a g a a c t t c c a c c t g t g t c t c c t c t t c a t g g t c c t c a a t a a g c t c t g t g g c t t t g t t c a c t t c t

6360 (in frame with SV40 NLS) NIKQFRDFKYSHRVDKKPNR NIKQFRDFKYSHRVDKKPNR a c a t c a a g c a g t t t a g a g a t t t c a a g t a t t c t c a c a g a g t t g a c a a g a a g c c c a a c c g g c t g t a g t t c g t c a a a t c t c t a a a g t t c a t a a g a g t g t c t c a a c t g t t c t t c g g g t t g g c c g

BspDI*ClaI* 6420 (in frame with SV40 NLS) QLINDTLYSTREIDGETYVV QLINDTLYSTREIDGETYVV a g c t g a t c a a t g a t a c c c t g t a c a g t a c c a g a g a g a t c g a t g g c g a a a c c t a c g t g g t c c t c g a c t a g t t a c t a t g g g a c a t g t c a t g g t c t c t c t a g c t a c c g c t t t g g a t g c a c c a g g

6480 (in frame with SV40 NLS) QTLKDLYAKDNEKVKKLFTE QTLKDLYAKDNEKVKKLFTE a a a c a c t g a a a g a c c t g t a c g c c a a g g a c a a t g a a a a g g t g a a a a a g c t c t t t a c a g a a c t t t g t g a c t t t c t g g a c a t g c g g t t c c t g t t a c t t t t c c a c t t t t t c g a g a a a t g t c t t g

6540 (in frame with SV40 NLS) RPQKILMYQHDPKTFEKLMT RPQKILMYQHDPKTFEKLMT g g c c t c a a a a g a t a c t g a t g t a c c a g c a c g a t c c t a a g a c c t t t g a g a a a c t g a t g a c c a c c g g a g t t t t c t a t g a c t a c a t g g t c g t g c t a g g a t t c t g g a a a c t c t t t g a c t a c t g g t

BbvCI 6600 (in frame with SV40 NLS) ILNQYAEAKNPLAAYYEDKG ILNQYAEAKNPLAAYYEDKG t t c t g a a t c a g t a c g c t g a g g c a a a g a a t c c t c t g g c c g c t t a t t a c g a g g a t a a g g g c g a a g a c t t a g t c a t g c g a c t c c g t t t c t t a g g a g a c c g g c g a a t a a t g c t c c t a t t c c c g c

6660 (in frame with SV40 NLS) EYVTKYAKKGNGPAIHKIKY EYVTKYAKKGNGPAIHKIKY a a t a c g t g a c c a a g t a c g c c a a g a a g g g c a a c g g c c c t g c c a t c c a c a a g a t c a a a t a c a t t a t g c a c t g g t t c a t g c g g t t c t t c c c g t t g c c g g g a c g g t a g g t g t t c t a g t t t a t g t

BmgBI 6720 (in frame with SV40 NLS) IDKKLGSYLDVSNKYPETQN IDKKLGSYLDVSNKYPETQN t c g a c a a g a a a c t g g g c a g c t a c c t g g a c g t g a g t a a c a a a t a t c c t g a g a c a c a g a a c a a g c t g t t c t t t g a c c c g t c g a t g g a c c t g c a c t c a t t g t t t a t a g g a c t c t g t g t c t t g t

6780 (in frame with SV40 NLS) KLVKLSLKSFRFDIYKCEQG KLVKLSLKSFRFDIYKCEQG a g c t g g t g a a a c t g t c t c t g a a g a g c t t t a g a t t c g a c a t c t a c a a a t g t g a a c a g g g c t t c g a c c a c t t t g a c a g a g a c t t c t c g a a a t c t a a g c t g t a g a t g t t t a c a c t t g t c c c g a

6840 (in frame with SV40 NLS) YKMVSIGYLDVLKKDNYYYI YKMVSIGYLDVLKKDNYYYI a c a a g a t g g t g t c c a t t g g c t a c c t c g a c g t a c t g a a g a a g g a c a a c t a c t a c t a c a t c c t g t t c t a c c a c a g g t a a c c g a t g g a g c t g c a t g a c t t c t t c c t g t t g a t g a t g a t g t a g g

6900 (in frame with SV40 NLS) PKDKYEAEKQKKKIKESDLF PKDKYEAEKQKKKIKESDLF c c a a a g a t a a g t a c g a g g c c g a g a a g c a g a a a a a g a a g a t c a a g g a a a g c g a c c t c t t c g g g t t t c t a t t c a t g c t c c g g c t c t t c g t c t t t t t c t t c t a g t t c c t t t c g c t g g a g a a g c

6960 (in frame with SV40 NLS) VGSFYYNDLIMYEDELFRVI VGSFYYNDLIMYEDELFRVI t g g g c a g c t t c t a c t a c a a c g a c c t g a t c a t g t a c g a g g a c g a a c t c t t c c g g g t g a t c g a c c c g t c g a a g a t g a t g t t g c t g g a c t a g t a c a t g c t c c t g c t t g a g a a g g c c c a c t a g c

SalIEcoRV 7020 (in frame with SV40 NLS) GVNSDINNLVELNMVDITYK GVNSDINNLVELNMVDITYK g a g t g a a c t c c g a t a t c a a c a a c c t g g t t g a g c t g a a t a t g g t c g a c a t c a c c t a c a a g g c t c a c t t g a g g c t a t a g t t g t t g g a c c a a c t c g a c t t a t a c c a g c t g t a g t g g a t g t t c c

7080 (in frame with SV40 NLS) DFCEVNNVTGEKRIKKTIGK DFCEVNNVTGEKRIKKTIGK a t t t c t g c g a g g t g a a c a a c g t g a c a g g c g a g a a g a g a a t c a a g a a a a c c a t c g g a a a g a t a a a g a c g c t c c a c t t g t t g c a c t g t c c g c t c t t c t c t t a g t t c t t t t g g t a g c c t t t c t

BstZ17I 7140 (in frame with SV40 NLS) RVVLIEKYTTDILGNLYKTP RVVLIEKYTTDILGNLYKTP g a g t g g t g c t g a t c g a g a a g t a t a c g a c c g a c a t c c t g g g a a a t c t g t a t a a a a c g c c c c c t c a c c a c g a c t a g c t c t t c a t a t g c t g g c t g t a g g a c c c t t t a g a c a t a t t t t g c g g g g

7200 (in frame with SV40 NLS) LPKKPQLIFKRGELSGGSKR LPKKPQLIFKRGELSGGSKR t g c c t a a g a a g c c c c a g c t c a t t t t c a a g a g a g g c g a g c t g t c t g g c g g c t c a a a a a g a a a c g g a t t c t t c g g g g t c g a g t a a a a g t t c t c t c c g c t c g a c a g a c c g c c g a g t t t t t c t t

7260 (in frame with SV40 NLS) TADGSEFE 130 PKKKRKV * SV40 NLS bGH poly(A) signal TADGSEFEPKKKRKV* c c g c c g a c g g c a g c g a a t t c g a g c c c a a g a a g a a g a g g a a a g t c t a a t a g a t c t c g a c t g g g c g g c t g c c g t c g c t t a a g c t c g g g t t c t t c t t c t c c t t t c a g a t t a t c t a g a g c t g a c

7320 bGH poly(A) signal t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c

7380 bGH poly(A) signal a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t

7440 bGH poly(A) signal g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c

HindIIIPaeR7IPspXIXhoISphI 7500 bGH poly(A) signal a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t c g a g c g g c c c a a g c t t t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a g c t c g c c g g g t t c g a a

7560 a a a a a a a t c t c g c c a a c a a g t t g a c g a g a t a a a c a c g g c a t t t t g c c t t g t t t t a g t a g a t t t t t t t a g a g c g g t t g t t c a a c t g c t c t a t t t g t g c c g t a a a a c g g a a c a a a a t c a t c t

NcoI 7620 U6 promoter t t c t g t a a t t t t c a t t a c a g a g t a c t a a a a c a c c c c g c c a t g g c t t c g a c c a c g g t g t t t a a g a c a t t a a a a g t a a t g t c t c a t g a t t t t g t g g g g c g g t a c c g a a g c t g g t g c c a c a a a

7680 U6 promoter T G C C A T T C G LKO.1 5' c g t c c t t t c c a c a a g a t a t a t a a a g c c a a g a a a t c g a a a t a c t t t c a a g t t a c g g t a a g c g c a g g a a a g g t g t t c t a t a t a t t t c g g t t c t t t a g c t t t a t g a a a g t t c a a t g c c a t t c g

7740 U6 promoter T A T A C T A T C A G LKO.1 5' a t a t g a t a g t c c a t t t t a a a a c a t a a t t t t a a a a c t g c a a a c t a c c c a a g a a a t t a t t a c t a t a c t a t c a g g t a a a a t t t t g t a t t a a a a t t t t g a c g t t t g a t g g g t t c t t t a a t a a t g

7800 U6 promoter t t t c t a c g t c a c g t a t t t t g t a c t a a t a t c t t t g t g t t t a c a g t c a a a t t a a t t c t a a t t a a a g a t g c a g t g c a t a a a a c a t g a t t a t a g a a a c a c a a a t g t c a g t t t a a t t a a g a t t a a

7860 U6 promoter T T A G T A C C C T T T A T C C G G G hU6-F a t c t c t c t a a c a g c c t t g t a t c g t a t a t g c a a a t a t g a a g g a a t c a t g g g a a a t a g g c c c t a g a g a g a t t g t c g g a a c a t a g c a t a t a c g t t t a t a c t t c c t t a g t a c c c t t t a t c c g g g

NotI 7920 U6 promoter A G hU6-F t c t t c c t g c c c g a c c t t g c g g c c g c a g g a a c c c c t a g t g a t g g a g t t g g c c a c t c c c t c t a g a a g g a c g g g c t g g a a c g c c g g c g t c c t t g g g g a t c a c t a c c t c a a c c g g t g a g g g a g a

7980 c t g c g c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c c g a c g c c c g g g c t t t g a c g c g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g g c t g c g g g c c c g a a a

8006 g c c c g g g c g g c c t c a g t g a g c g a g c g c g g g c c c g c c g g a g t c a c t c g c t c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.