Addgene AA066 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: AA066 Sequencing Result


Map View

pRS-marker (151 .. 170) pGEX 3' (29 .. 51) PfoI (46) BtgZI (3799) BsrDI (3780) pENTR-R (3694 .. 3713) Kan-R (3616 .. 3635) SspI (3351) AsiSI (3278) PflMI (3016) AlwNI (2482) BciVI (2269) BssSI - BssSαI (2239) pBR322ori-F (2207 .. 2226) AflIII - PciI (2066) L4440 (1956 .. 1973) BspQI - SapI (1950) CAP binding site M13/pUC Reverse (1738 .. 1760) TatI (167) NdeI (184) BstAPI (185) BglI (251) FspI (258) M13/pUC Forward (364 .. 386) EcoRI (396) Eco53kI (404) SacI (406) Acc65I (408) KpnI (412) XbaI (425) MslI (427) SacII (474) BseRI (476) AfeI (499) DraIII (841) BsaI (910) EcoO109I - KflI - PpuMI (1157) BpmI (1205) ZraI (1288) AatII (1290) PsiI (1309) BfuAI - BspMI - PaqCI (1336) AvrII (1382) AflII (1482) hUBCpro-F (1492 .. 1511) SpeI (1605) BamHI (1671) TspMI - XmaI (1675) SmaI (1677) PspOMI (1678) ApaI (1682) SalI (1684) AccI (1685) HincII (1686) PstI (1693) SphI (1705) HindIII (1707) M13 rev M13 Reverse (1725 .. 1741) lac operator AA066 3815 bp

Sequence View

PfoI 60 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t

120 c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c

TatI 180 C G G C A T C A G A G C A G A T T G T A pRS-marker t t g g c g g g t g t c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a a c c g c c c a c a g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g

NdeIBstAPI 240 a c c a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g c g c c t g g t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c g c g g

FspIBglI 300 a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a

360 t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a

EcoRIEco53kISacIAcc65IKpnI 420 C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g a g a a t t c g a g c t c g g t a c c t c g c g a a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c t c t t a a g c t c g a g c c a t g g a g c g c t t

SacIIBseRIXbaIMslI 480 UbC promoter t a c a t c t a g a t g a a g a c g t a c a c g g c c t c c g c g c c g g g t t t t g g c g c c t c c c g c g g g c g c a t g t a g a t c t a c t t c t g c a t g t g c c g g a g g c g c g g c c c a a a a c c g c g g a g g g c g c c c g c g

AfeI 540 UbC promoter c c c c c t c c t c a c g g c g a g c g c t g c c a c g t c a g a c g a a g g g c g c a g c g a g c g t c c t g a t c c g g g g g a g g a g t g c c g c t c g c g a c g g t g c a g t c t g c t t c c c g c g t c g c t c g c a g g a c t a g g

600 UbC promoter t t c c g c c c g g a c g c t c a g g a c a g c g g c c c g c t g c t c a t a a g a c t c g g c c t t a g a a c c c c a a a g g c g g g c c t g c g a g t c c t g t c g c c g g g c g a c g a g t a t t c t g a g c c g g a a t c t t g g g g t

660 UbC promoter g t a t c a g c a g a a g g a c a t t t t a g g a c g g g a c t t g g g t g a c t c t a g g g c a c t g g t t t t c t t c a t a g t c g t c t t c c t g t a a a a t c c t g c c c t g a a c c c a c t g a g a t c c c g t g a c c a a a a g a a

720 UbC promoter t c c a g a g a g c g g a a c a g g c g a g g a a a a g t a g t c c c t t c t c g g c g a t t c t g c g g a g g g a t c a g g t c t c t c g c c t t g t c c g c t c c t t t t c a t c a g g g a a g a g c c g c t a a g a c g c c t c c c t a g

780 UbC promoter t c c g t g g g g c g g t g a a c g c c g a t g a t t a t a t a a g g a c g c g c c g g g t g t g g c a c a g c t a g t a g g c a c c c c g c c a c t t g c g g c t a c t a a t a t a t t c c t g c g c g g c c c a c a c c g t g t c g a t c a

840 UbC promoter t c c g t c g c a g c c g g g a t t t g g g t c g c a g t t c t t g t t t g t g g a t c g c t g t g a t c g t c a c t t a g g c a g c g t c g g c c c t a a a c c c a g c g t c a a g a a c a a a c a c c t a g c g a c a c t a g c a g t g a a

DraIII 900 UbC promoter g g t g a g t a g c g g g c t g c t g g g c t g g c c g g g g c t t t c g t g g c c g c c g g g c c g c t c g g t g g g c c a c t c a t c g c c c g a c g a c c c g a c c g g c c c c g a a a g c a c c g g c g g c c c g g c g a g c c a c c c

BsaI 960 UbC promoter a c g g a g g c g t g t g g a g a g a c c g c c a a g g g c t g t a g t c t g g g t c c g c g a g c a a g g t t g c c c t g c c t c c g c a c a c c t c t c t g g c g g t t c c c g a c a t c a g a c c c a g g c g c t c g t t c c a a c g g g

1020 UbC promoter t g a a c t g g g g g t t g g g g g g a g c g c a g c a a a a t g g c g g c t g t t c c c g a g t c t t g a a t g g a a a c t t g a c c c c c a a c c c c c c t c g c g t c g t t t t a c c g c c g a c a a g g g c t c a g a a c t t a c c t t

1080 UbC promoter g a t g c t t g t g a g g c g g g c t g t g a g g t c g t t g a a a c a a g g t g g g g g g c a t g g t g g g c g g c a c t a c g a a c a c t c c g c c c g a c a c t c c a g c a a c t t t g t t c c a c c c c c c g t a c c a c c c g c c g t

1140 UbC promoter a g a a c c c a a g g t c t t g a g g c c t t c g c t a a t g c g g g a a a g c t c t t a t t c g g g t g a g a t g g g t c t t g g g t t c c a g a a c t c c g g a a g c g a t t a c g c c c t t t c g a g a a t a a g c c c a c t c t a c c c

EcoO109IPpuMIKflI 1200 UbC promoter c t g g g g c a c c a t c t g g g g a c c c t g a c g t g a a g t t t g t c a c t g a c t g g a g a a c t c g g t t t g g a c c c c g t g g t a g a c c c c t g g g a c t g c a c t t c a a a c a g t g a c t g a c c t c t t g a g c c a a a c

BpmI 1260 UbC promoter t c g t c t g t t g c g g g g g c g g c a g t t a t g g c g g t g c c g t t g g g c a g t g c a c c c g t a c c t t t g a g c a g a c a a c g c c c c c g c c g t c a a t a c c g c c a c g g c a a c c c g t c a c g t g g g c a t g g a a a c

PsiIZraIAatII 1320 UbC promoter g g a g c g c g c g c c c t c g t c g t g t c g t g a c g t c a c c c g t t c t g t t g g c t t a t a a t g c a g g g t c c t c g c g c g c g g g a g c a g c a c a g c a c t g c a g t g g g c a a g a c a a c c g a a t a t t a c g t c c c a

BspMIPaqCIBfuAI 1380 UbC promoter g g g g c c a c c t g c c g g t a g g t g t g c g g t a g g c t t t t c t c c g t c g c a g g a c g c a g g g t t c g g c c c c g g t g g a c g g c c a t c c a c a c g c c a t c c g a a a a g a g g c a g c g t c c t g c g t c c c a a g c c

AvrII 1440 UbC promoter g c c t a g g g t a g g c t c t c c t g a a t c g a c a g g c g c c g g a c c t c t g g t g a g g g g a g g g a t a a g c g g a t c c c a t c c g a g a g g a c t t a g c t g t c c g c g g c c t g g a g a c c a c t c c c c t c c c t a t t c

AflII 1500 UbC promoter T G A A G C T C C hUBCpro-F t g a g g c g t c a g t t t c t c t g g t c g g t t t t a t g t a c c t a t c t t c t t a a g t a g c t g a a g c t c c a c t c c g c a g t c a a a g a g a c c a g c c a a a a t a c a t g g a t a g a a g a a t t c a t c g a c t t c g a g g

1560 UbC promoter G G T T T T G A A C T hUBCpro-F g g t t t t g a a c t a t g c g c t c g g g g t t g g c g a g t g t g t t t t g t g a a g t t t t t t a g g c a c c t t c c a a a a c t t g a t a c g c g a g c c c c a a c c g c t c a c a c a a a a c a c t t c a a a a a a t c c g t g g a a

SpeI 1620 UbC promoter t t g a a a t g t a a t c a t t t g g g t c a a t a t g t a a t t t t c a g t g t t a g a c t a g t a a a t t g t c c g a a c t t t a c a t t a g t a a a c c c a g t t a t a c a t t a a a a g t c a c a a t c t g a t c a t t t a a c a g g c

BamHITspMIXmaISmaIPspOMI 1680 UbC promoter c t a a a t t c t g g c c g t t t t t g g c t t t t t t g t t a g a c a g g a g c g t c t t c a t c g g a t c c c g g g g a t t t a a g a c c g g c a a a a a c c g a a a a a a c a a t c t g t c c t c g c a g a a g t a g c c t a g g g c c c

SphIHindIIIPstIApaISalIAccIHincII 1740 M13 rev C A G T A T C G A C A A A G G A M13 Reverse G G A M13/pUC Reverse c c c g t c g a c t g c a g a g g c c t g c a t g c a a g c t t g g t g t a a t c a t g g t c a t a g c t g t t t c c t g g g c a g c t g a c g t c t c c g g a c g t a c g t t c g a a c c a c a t t a g t a c c a g t a t c g a c a a a g g a

1800 -10 -35 M13 rev lac operator lac promoter C M13 Reverse C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a

1860 -35 lac promoter CAP binding site a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g

1920 g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c

BspQISapI 1980 G A G C G A G T G A C T G A G C G A L4440 a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c

2040 g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t

AflIIIPciI 2100 g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g

2160 ori c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g

2220 ori T T T C T A T G G T C C G C pBR322ori-F a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c

BciVIBssSαIBssSI 2280 ori A A A G G G pBR322ori-F t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g

2340 ori c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a

2400 ori c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c

2460 ori c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g

AlwNI 2520 ori t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a

2580 ori g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a

2640 ori a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g

2700 ori a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t

2760 ori a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g

2820 g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g

2880 c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a g c c c a a t c t g a a t a a t g t t a c a a c c a a t t g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t c g g g t t a g a c t t a t t a c a a t g t t g g t t a a

2940 270 265 260 255 *FFEDLMLHFQLKNMD KanR *FFEDLMLHFQLKNMD a a c c a a t t c t g a t t a g a a a a a c t c a t c g a g c a t c a a a t g a a a c t g c a a t t t a t t c a t a t c t t g g t t a a g a c t a a t c t t t t t g a g t a g c t c g t a g t t t a c t t t g a c g t t a a a t a a g t a t a g

3000 250 245 240 235 PNDIGYKQFLRKQLSPSFEG KanR PNDIGYKQFLRKQLSPSFEG a g g a t t a t c a a t a c c a t a t t t t t g a a a a a g c c g t t t c t g t a a t g a a g g a g a a a a c t c a c c t c c t a a t a g t t a t g g t a t a a a a a c t t t t t c g g c a a a g a c a t t a c t t c c t c t t t t g a g t g g

PflMI 3060 230 225 220 215 LCNWLIALDQYRDAIGVRGV KanR LCNWLIALDQYRDAIGVRGV g a g g c a g t t c c a t a g g a t g g c a a g a t c c t g g t a t c g g t c t g c g a t t c c g a c t c g t c c a a c c t c c g t c a a g g t a t c c t a c c g t t c t a g g a c c a t a g c c a g a c g c t a a g g c t g a g c a g g t t g

3120 210 205 200 195 DICGILKGEDFILNDLSFDG KanR DICGILKGEDFILNDLSFDG a t c a a t a c a a c c t a t t a a t t t c c c c t c g t c a a a a a t a a g g t t a t c a a g t g a g a a a t c a c c t a g t t a t g t t g g a t a a t t a a a g g g g a g c a g t t t t t a t t c c a a t a g t t c a c t c t t t a g t g g

3180 190 185 180 175 HTVVSDPSFPLLKHMEKWVQ KanR HTVVSDPSFPLLKHMEKWVQ a t g a g t g a c g a c t g a a t c c g g t g a g a a t g g c a a a a g t t t a t g c a t t t c t t t c c a g a c t t g t a c t c a c t g c t g a c t t a g g c c a c t c t t a c c g t t t t c a a a t a c g t a a a g a a a g g t c t g a a c

3240 170 165 160 155 EVPWGNREDDFDSADVLGNN KanR EVPWGNREDDFDSADVLGNN t t c a a c a g g c c a g c c a t t a c g c t c g t c a t c a a a a t c a c t c g c a t c a a c c a a a c c g t t a t t a a g t t g t c c g g t c g g t a a t g c g a g c a g t a g t t t t a g t g a g c g t a g t t g g t t t g g c a a t a a

AsiSI 3300 150 145 140 135 MRSQAQALRFVRDSNFPCNC KanR MRSQAQALRFVRDSNFPCNC c a t t c g t g a t t g c g c c t g a g c g a g a c g a a a t a c g c g a t c g c t g t t a a a a g g a c a a t t a c a g t a a g c a c t a a c g c g g a c t c g c t c t g c t t t a t g c g c t a g c g a c a a t t t t c c t g t t a a t g t

SspI 3360 130 125 120 115 VPISHLRRLFVALADVINEG KanR VPISHLRRLFVALADVINEG a a c a g g a a t c g a a t g c a a c c g g c g c a g g a a c a c t g c c a g c g c a t c a a c a a t a t t t t c a c c t t g t c c t t a g c t t a c g t t g g c c g c g t c c t t g t g a c g g t c g c g t a g t t g t t a t a a a a g t g g

3420 110 105 100 95 SDPYEELVQFATKGPIATTL KanR SDPYEELVQFATKGPIATTL t g a a t c a g g a t a t t c t t c t a a t a c c t g g a a t g c t g t t t t t c c g g g g a t c g c a g t g g t g a g a c t t a g t c c t a t a a g a a g a t t a t g g a c c t t a c g a c a a a a a g g c c c c t a g c g t c a c c a c t c

3480 90 85 80 75 LWADDPTRIFHKITPLPMFE KanR LWADDPTRIFHKITPLPMFE t a a c c a t g c a t c a t c a g g a g t a c g g a t a a a a t g c t t g a t g g t c g g a a g a g g c a t a a a t t c a t t g g t a c g t a g t a g t c c t c a t g c c t a t t t t a c g a a c t a c c a g c c t t c t c c g t a t t t a a g

3540 70 65 60 55 TLWNLRVMEDTVDNAVSGKG KanR TLWNLRVMEDTVDNAVSGKG c g t c a g c c a g t t t a g t c t g a c c a t c t c a t c t g t a a c a t c a t t g g c a a c g c t a c c t t t g c c g c a g t c g g t c a a a t c a g a c t g g t a g a g t a g a c a t t g t a g t a a c c g t t g c g a t g g a a a c g g

3600 50 45 40 35 HKLFLEPADPKGYLRYITAG KanR HKLFLEPADPKGYLRYITAG a t g t t t c a g a a a c a a c t c t g g c g c a t c g g g c t t c c c a t a c a a g c g a t a g a t t g t c g c a c c t a c a a a g t c t t t g t t g a g a c c g c g t a g c c c g a a g g g t a t g t t c g c t a t c t a a c a g c g t g g

3660 30 25 20 15 SQGVNDRAWKYGYLDADMNS KanR A T C G C G A G C C C A T T T A T A C C Kan-R SQGVNDRAWKYGYLDADMNS t g a t t g c c c g a c a t t a t c g c g a g c c c a t t t a t a c c c a t a t a a a t c a g c a t c c a t g t t g g a a c t a a c g g g c t g t a a t a g c g c t c g g g t a a a t a t g g g t a t a t t t a g t c g t a g g t a c a a c c t

3720 10 5 1 NLRPRSTERQIHSM KanR A T G G C T C A T A A C A C C C C T T G pENTR-R NLRPRSTERQIHSM a t t t a a t c g c g g c c t c g a c g t t t c c c g t t g a a t a t g g c t c a t a a c a c c c c t t g t a t t a c t t a a a t t a g c g c c g g a g c t g c a a a g g g c a a c t t a t a c c g a g t a t t g t g g g g a a c a t a a t g a

BsrDI 3780 g t t t a t g t a a g c a g a c a g t t t t a t t g t t c a t g a t g a t a t a t t t t t a t c t t g t g c a a t g t a c a a a t a c a t t c g t c t g t c a a a a t a a c a a g t a c t a c t a t a t a a a a a t a g a a c a c g t t a c a t

BtgZI 3815 a c a t c a g a g a t t t t g a g a c a c g g g c c a g a g c t g c a t g t a g t c t c t a a a a c t c t g t g c c c g g t c t c g a c g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.