Addgene HA-MBP-TEVpCS-ADGRG3 CTF-FLAG Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: HA-MBP-TEVpCS-ADGRG3 CTF-FLAG Sequencing Result


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pause site RVprimer3 (6207 .. 6226) poly(A) signal pBR322ori-F (5492 .. 5511) AflIII - PciI (5351) SnaBI (5052) SpeI (4922) ApaI (4918) PspOMI (4914) MauBI (4395) FseI (4163) DraIII (4085) SspI (3889) SV40pA-R (3494 .. 3513) EBV-rev (3440 .. 3459) BmtI (3414) NheI (3410) AsiSI - PvuI (11) PvuII (242) EcoRI (626) HA NcoI (1624) FspAI (1695) PflMI (1733) PmlI (1964) Acc65I (2223) KpnI (2227) PshAI (2430) Eco53kI (2485) SacI (2487) AccI (2545) BspHI * (2815) HA-MBP-TEVpCS-ADGRG3 CTF-FLAG 6264 bp

Sequence View

AsiSIPvuI 60 EF-1 α core promoter g g a t c t g c g a t c g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g c c t a g a c g c t a g c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c

120 EF-1 α core promoter a g a a g t t g g g g g g a g g g g t c g g c a a t t g a a c g g g t g c c t a g a g a a g g t g g c g c g g g g t a a t c t t c a a c c c c c c t c c c c a g c c g t t a a c t t g c c c a c g g a t c t c t t c c a c c g c g c c c c a t t

180 EF-1 α core promoter a c t g g g a a a g t g a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t t g a c c c t t t c a c t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a

240 EF-1 α core promoter a t a t a a g t g c a g t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c t a t a t t c a c g t c a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g

PvuII 300 EF-1 α core promoter 5' LTR (truncated) a g c t g a a g c t t c g a g g g g c t c g c a t c t c t c c t t c a c g c g c c c g c c g c c c t a c c t g a g g c c t c g a c t t c g a a g c t c c c c g a g c g t a g a g a g g a a g t g c g c g g g c g g c g g g a t g g a c t c c g g

360 5' LTR (truncated) g c c a t c c a c g c c g g t t g a g t c g c g t t c t g c c g c c t c c c g c c t g t g g t g c c t c c t g a a c t g c g g t a g g t g c g g c c a a c t c a g c g c a a g a c g g c g g a g g g c g g a c a c c a c g g a g g a c t t g a c

420 5' LTR (truncated) c g t c c g c c g t c t a g g t a a g t t t a a a g c t c a g g t c g a g a c c g g g c c t t t g t c c g g c g c t c c g c a g g c g g c a g a t c c a t t c a a a t t t c g a g t c c a g c t c t g g c c c g g a a a c a g g c c g c g a g g

480 5' LTR (truncated) c t t g g a g c c t a c c t a g a c t c a g c c g g c t c t c c a c g c t t t g c c t g a c c c t g c t t g c t c a a c g a a c c t c g g a t g g a t c t g a g t c g g c c g a g a g g t g c g a a a c g g a c t g g g a c g a a c g a g t t g

540 5' LTR (truncated) t c t a c g t c t t t g t t t c g t t t t c t g t t c t g c g c c g t t a c a g a t c c a a g c t g t g a c c g g c g c a g a t g c a g a a a c a a a g c a a a a g a c a a g a c g c g g c a a t g t c t a g g t t c g a c a c t g g c c g c g

600 1 5 MYRMQLLSC IL-2 signal sequence MYRMQLLSC c t a c c t g a g a t c a c c g g c g a a g g a g g g c c a c c a t g t a c a g g a t g c a a c t c c t g t c t t g c a g a t g g a c t c t a g t g g c c g c t t c c t c c c g g t g g t a c a t g t c c t a c g t t g a g g a c a g a a c g t

EcoRI 660 10 15 20 IALSLALVTNS 1 5 YPYDVPDYA IL-2 signal sequence HA IALSLALVTNSYPYDVPDYA *TKCKDRIRVWVINWIVS t t g c a c t a a g t c t t g c a c t t g t c a c g a a t t c g t a c c c a t a c g a t g t t c c a g a t t a c g c t g a a c g t g a t t c a g a a c g t g a a c a g t g c t t a a g c a t g g g t a t g c t a c a a g g t c t a a t g c g a c

720 (in frame with HA) GSKIEEGKLVIWINGDKGYN GSKIEEGKLVIWINGDKGYN SGFNFFAFQHNPNVAIFAIV g a t c c a a a a t t g a a g a a g g c a a a c t g g t g a t t t g g a t t a a c g g c g a t a a a g g c t a t a a c g c t a g g t t t t a a c t t c t t c c g t t t g a c c a c t a a a c c t a a t t g c c g c t a t t t c c g a t a t t g c

780 (in frame with HA) GLAEVGKKFEKDTGIKVTVE GLAEVGKKFEKDTGIKVTVE AQRFHAFFKFFIGANFHGHF g c c t g g c g g a a g t g g g c a a a a a a t t t g a a a a a g a t a c c g g c a t t a a a g t g a c c g t g g a a c c g g a c c g c c t t c a c c c g t t t t t t a a a c t t t t t c t a t g g c c g t a a t t t c a c t g g c a c c t t g

840 (in frame with HA) HPDKLEEKFPQVAATGDGPD HPDKLEEKFPQVAATGDGPD MRIFQFFFKRLHRRGAIARI a t c c g g a t a a a c t g g a a g a a a a a t t t c c g c a g g t g g c g g c g a c c g g c g a t g g c c c g g a t a t a g g c c t a t t t g a c c t t c t t t t t a a a g g c g t c c a c c g c c g c t g g c c g c t a c c g g g c c t a t

900 (in frame with HA) IIFWAHDRFGGYAQSGLLAE IIFWAHDRFGGYAQSGLLAE NNKPRMIAKAAIRLAAQQRF t t a t t t t t t g g g c g c a t g a t c g c t t t g g c g g c t a t g c g c a g a g c g g c c t g c t g g c g g a a a a a t a a a a a a c c c g c g t a c t a g c g a a a c c g c c g a t a c g c g t c t c g c c g g a c g a c c g c c t t t

960 (in frame with HA) ITPDKAFQDKLYPFTWDAVR ITPDKAFQDKLYPFTWDAVR NGRIFRKLIFQIRKGPIRHA t t a c c c c g g a t a a a g c g t t t c a g g a t a a a c t g t a t c c g t t t a c c t g g g a t g c g g t g c g c t a a t g g g g c c t a t t t c g c a a a g t c c t a t t t g a c a t a g g c a a a t g g a c c c t a c g c c a c g c g a

1020 (in frame with HA) YNGKLIAYPIAVEALSLIYN YNGKLIAYPIAVEALSLIYN IVAFQNRIRNRHFRQAQNIV a t a a c g g c a a a c t g a t t g c g t a t c c g a t t g c g g t g g a a g c g c t g a g c c t g a t t t a t a a c a t a t t g c c g t t t g a c t a a c g c a t a g g c t a a c g c c a c c t t c g c g a c t c g g a c t a a a t a t t g t

1080 (in frame with HA) KDLLPNPPKTWEEIPALDKE KDLLPNPPKTWEEIPALDKE FIQQRVRRFGPFFNRRQIFF a a g a t c t g c t g c c g a a c c c g c c g a a a a c c t g g g a a g a a a t t c c g g c g c t g g a t a a a g a a c t t c t a g a c g a c g g c t t g g g c g g c t t t t g g a c c c t t c t t t a a g g c c g c g a c c t a t t t c t t g

1140 (in frame with HA) LKAKGKSALMFNLQEPYFTW LKAKGKSALMFNLQEPYFTW QFRFAFARQHKVQLFRIKGP t g a a a g c g a a a g g c a a a a g c g c g c t g a t g t t t a a c c t g c a g g a a c c g t a t t t t a c c t g g c a c t t t c g c t t t c c g t t t t c g c g c g a c t a c a a a t t g g a c g t c c t t g g c a t a a a a t g g a c c g

1200 (in frame with HA) PLIAADGGYAFKYENGKYDI PLIAADGGYAFKYENGKYDI RQNRRIAAIRKFIFVAFIIN c g c t g a t t g c g g c g g a t g g c g g c t a t g c g t t t a a a t a t g a a a a c g g c a a a t a t g a t a t t a g c g a c t a a c g c c g c c t a c c g c c g a t a c g c a a a t t t a t a c t t t t g c c g t t t a t a c t a t a a t

1260 (in frame with HA) KDVGVDNAGAKAGLTFLVDL KDVGVDNAGAKAGLTFLVDL FIHAHIVRARFRAQGKQHIQ a a g a t g t g g g c g t g g a t a a c g c g g g c g c g a a a g c g g g c c t g a c c t t t c t g g t g g a t c t g a t t c t a c a c c c g c a c c t a t t g c g c c c g c g c t t t c g c c c g g a c t g g a a a g a c c a c c t a g a c t

1320 (in frame with HA) IKNKHMNADTDYSIAEAAFN IKNKHMNADTDYSIAEAAFN NFVFM t t a a a a a c a a a c a t a t g a a c g c g g a t a c c g a t t a t a g c a t t g c g g a a g c g g c g t t t a a c a a a t t t t t g t t t g t a t a c t t g c g c c t a t g g c t a a t a t c g t a a c g c c t t c g c c g c a a a t t g t

1380 (in frame with HA) KGETAMTINGPWAWSNIDTS KGETAMTINGPWAWSNIDTS a a g g c g a a a c c g c g a t g a c c a t t a a c g g c c c g t g g g c g t g g a g c a a c a t t g a t a c c a g c a t t c c g c t t t g g c g c t a c t g g t a a t t g c c g g g c a c c c g c a c c t c g t t g t a a c t a t g g t c g t

1440 (in frame with HA) KVNYGVTVLPTFKGQPSKPF KVNYGVTVLPTFKGQPSKPF a a g t g a a c t a t g g c g t g a c c g t g c t g c c g a c c t t t a a a g g c c a g c c g a g c a a a c c g t t t g t t c a c t t g a t a c c g c a c t g g c a c g a c g g c t g g a a a t t t c c g g t c g g c t c g t t t g g c a a a c

1500 (in frame with HA) VGVLSAGINAASPNKELAKE VGVLSAGINAASPNKELAKE t g g g c g t g c t g a g c g c g g g c a t t a a c g c g g c g a g c c c g a a c a a a g a a c t g g c g a a a g a a t a c c c g c a c g a c t c g c g c c c g t a a t t g c g c c g c t c g g g c t t g t t t c t t g a c c g c t t t c t t a

1560 (in frame with HA) FLENYLLTDEGLEAVNKDKP FLENYLLTDEGLEAVNKDKP t t c t g g a a a a c t a t c t g c t g a c c g a t g a a g g c c t g g a a g c g g t g a a c a a a g a t a a a c c g c a a g a c c t t t t g a t a g a c g a c t g g c t a c t t c c g g a c c t t c g c c a c t t g t t t c t a t t t g g c g

1620 (in frame with HA) LGAVALKSYEEELAKDPRIA LGAVALKSYEEELAKDPRIA t g g g c g c g g t g g c g c t g a a a a g c t a t g a a g a a g a a c t g g c g a a a g a t c c g c g c a t t g c g g a c c c g c g c c a c c g c g a c t t t t c g a t a c t t c t t c t t g a c c g c t t t c t a g g c g c g t a a c g c c

NcoI 1680 (in frame with HA) ATMENAQKGEIMPNIPQMSA ATMENAQKGEIMPNIPQMSA c g a c c a t g g a a a a c g c g c a g a a a g g c g a a a t t a t g c c g a a c a t t c c g c a g a t g a g c g c g t g c t g g t a c c t t t t g c g c g t c t t t c c g c t t t a a t a c g g c t t g t a a g g c g t c t a c t c g c g c a

PflMIFspAI 1740 (in frame with HA) FWYAVRTAVINAASGRQTVD FWYAVRTAVINAASGRQTVD t t t g g t a t g c g g t g c g c a c c g c g g t g a t t a a c g c g g c g a g c g g c c g c c a g a c c g t g g a t g a a a c c a t a c g c c a c g c g t g g c g c c a c t a a t t g c g c c g c t c g c c g g c g g t c t g g c a c c t a c

1800 (in frame with HA) EALKDAQTNENLYFQTFFAL EALKDAQTNENLYFQTFFAL a a g c g c t g a a a g a t g c g c a g a c c a a c g a g a a c c t t t a c t t c c a a a c g t t t t t c g c c c t g c t t c g c g a c t t t c t a c g c g t c t g g t t g c t c t t g g a a a t g a a g g t t t g c a a a a a g c g g g a c g

1860 (in frame with HA) LLRPTLDQSTVHILTRISQA LLRPTLDQSTVHILTRISQA t c c t g c g c c c c a c t c t t g a t c a g t c a a c c g t t c a c a t c c t t a c c c g c a t c t c t c a g g c t g a g g a c g c g g g g t g a g a a c t a g t c a g t t g g c a a g t g t a g g a a t g g g c g t a g a g a g t c c g a c

1920 (in frame with HA) GCGVSMIFLAFTIILYAFLR GCGVSMIFLAFTIILYAFLR g c t g c g g c g t g a g c a t g a t c t t c c t g g c a t t c a c c a t t a t c c t g t a t g c t t t c c t g c g t c c g a c g c c g c a c t c g t a c t a g a a g g a c c g t a a g t g g t a a t a g g a c a t a c g a a a g g a c g c a g

PmlI 1980 (in frame with HA) LSRERFKSEDAPKIHVALGG LSRERFKSEDAPKIHVALGG t g t c c c g c g a g c g c t t t a a a a g c g a g g a t g c c c c c a a g a t t c a c g t g g c t c t g g g c g g g a a c a g g g c g c t c g c g a a a t t t t c g c t c c t a c g g g g g t t c t a a g t g c a c c g a g a c c c g c c c t

2040 (in frame with HA) SLFLLNLAFLVNVGSGSKGS SLFLLNLAFLVNVGSGSKGS g c c t g t t c c t g c t c a a t t t g g c t t t t c t g g t t a a c g t g g g a t c c g g a t c a a a g g g t a g c g c g g a c a a g g a c g a g t t a a a c c g a a a a g a c c a a t t g c a c c c t a g g c c t a g t t t c c c a t c g c

2100 (in frame with HA) DAACWARGAVFHYFLLCAFT DAACWARGAVFHYFLLCAFT a c g c c g c t t g c t g g g c g a g g g g c g c c g t g t t t c a c t a c t t c c t c c t g t g t g c t t t c a c t t t g c g g c g a a c g a c c c g c t c c c c g c g g c a c a a a g t g a t g a a g g a g g a c a c a c g a a a g t g a a

2160 (in frame with HA) WMGLEAFHLYLLAVRVFNTY WMGLEAFHLYLLAVRVFNTY g g a t g g g c t t g g a g g c c t t c c a t c t g t a c c t c c t g g c t g t t c g c g t g t t c a a c a c t t a t t c c t a c c c g a a c c t c c g g a a g g t a g a c a t g g a g g a c c g a c a a g c g c a c a a g t t g t g a a t a a

2220 (in frame with HA) FGHYFLKLSLVGWGLPALMV FGHYFLKLSLVGWGLPALMV t c g g c c a t t a t t t c c t g a a g c t t t c c c t c g t g g g t t g g g g c c t g c c g g c c c t g a t g g t g a a g c c g g t a a t a a a g g a c t t c g a a a g g g a g c a c c c a a c c c c g g a c g g c c g g g a c t a c c a c t

Acc65IKpnI 2280 (in frame with HA) IGTGSANSYGLYTIRDRENR IGTGSANSYGLYTIRDRENR t c g g t a c c g g c t c c g c a a a t t c c t a c g g a c t g t a c a c a a t c c g c g a c a g a g a a a a c c g c a a g c c a t g g c c g a g g c g t t t a a g g a t g c c t g a c a t g t g t t a g g c g c t g t c t c t t t t g g c g t

2340 (in frame with HA) TSLELCWFREGTTMYALYIT TSLELCWFREGTTMYALYIT c g a g c c t g g a a c t g t g t t g g t t c c g c g a g g g c a c g a c c a t g t a c g c c c t g t a c a t c a c c g g c t c g g a c c t t g a c a c a a c c a a g g c g c t c c c g t g c t g g t a c a t g c g g g a c a t g t a g t g g c

2400 (in frame with HA) VHGYFLITFLFGMVVLALVV VHGYFLITFLFGMVVLALVV t g c a c g g a t a t t t c c t g a t c a c a t t c c t g t t t g g a a t g g t c g t g c t g g c t c t t g t g g t c t a c g t g c c t a t a a a g g a c t a g t g t a a g g a c a a a c c t t a c c a g c a c g a c c g a g a a c a c c a g a

PshAI 2460 (in frame with HA) WKIFTLSRATAVKERGKNRK WKIFTLSRATAVKERGKNRK g g a a g a t c t t c a c t c t c t c c c g g g c g a c c g c g g t c a a g g a g c g t g g t a a g a a t a g g a a g a c c t t c t a g a a g t g a g a g a g g g c c c g c t g g c g c c a g t t c c t c g c a c c a t t c t t a t c c t t c t

Eco53kISacI 2520 (in frame with HA) KVLTLLGLSSLVGVTWGLAI KVLTLLGLSSLVGVTWGLAI a a g t c c t g a c t c t t c t g g g c c t g a g c t c c c t g g t g g g c g t g a c t t g g g g t c t g g c c a t c t t t c a g g a c t g a g a a g a c c c g g a c t c g a g g g a c c a c c c g c a c t g a a c c c c a g a c c g g t a g a

AccI 2580 (in frame with HA) FTPLGLSTVYIFALFNSLQG FTPLGLSTVYIFALFNSLQG t c a c t c c a c t c g g c c t g a g c a c t g t c t a c a t c t t c g c c t t g t t t a a t t c c t t g c a g g g t g a g t g a g g t g a g c c g g a c t c g t g a c a g a t g t a g a a g c g g a a c a a a t t a a g g a a c g t c c c a c

2640 (in frame with HA) VFICCWFTILYLPSQSTTVS VFICCWFTILYLPSQSTTVS t a t t c a t c t g c t g t t g g t t t a c t a t c c t c t a c c t g c c g t c c c a g t c c a c a a c t g t c t c c t a t a a g t a g a c g a c a a c c a a a t g a t a g g a g a t g g a c g g c a g g g t c a g g t g t t g a c a g a g g a

2700 (in frame with HA) SSTARLDQAHSASQEGS 1 DYK FLAG SSTARLDQAHSASQEGSDYK c t t c c a c c g c t a g a c t g g a t c a g g c g c a c t c t g c t t c t c a g g a a g g t t c t g a c t a c a a a g g a a g g t g g c g a t c t g a c c t a g t c c g c g t g a g a c g a a g a g t c c t t c c a a g a c t g a t g t t t c

2760 5 DDDDK * FLAG DDDDK* a c g a t g a c g a c a a g t g a a g a t c t g a c a a a a c t c a c a c a t g c c c a c c g t g c c c a g c a c c t g t g c t a c t g c t g t t c a c t t c t a g a c t g t t t t g a g t g t g t a c g g g t g g c a c g g g t c g t g g a c

BspHI* 2820 M a a c t c c t g g g g g g a c c g t c a g t c t t c c t c t t c c c c c c a a a a c c c a a g g a c a c c c t c a t g a t t g a g g a c c c c c c t g g c a g t c a g a a g g a g a a g g g g g g t t t t g g g t t c c t g t g g g a g t a c t

2880 ISRTPEVTCVVVDVSHEDPE t c t c c c g g a c c c c t g a g g t c a c a t g c g t g g t g g t g g a c g t g a g c c a c g a a g a c c c t g a g g a g a g g g c c t g g g g a c t c c a g t g t a c g c a c c a c c a c c t g c a c t c g g t g c t t c t g g g a c t c c

2940 VKFNWYVDGVEVHNAKTKPR t c a a g t t c a a c t g g t a c g t g g a c g g c g t g g a g g t g c a t a a t g c c a a g a c a a a g c c g c g g g a g t t c a a g t t g a c c a t g c a c c t g c c g c a c c t c c a c g t a t t a c g g t t c t g t t t c g g c g c c c

3000 EEQYNSTYRVVSVLTVLHQD a g g a g c a g t a c a a c a g c a c g t a c c g t g t g g t c a g c g t c c t c a c c g t c c t g c a c c a g g a c t t c c t c g t c a t g t t g t c g t g c a t g g c a c a c c a g t c g c a g g a g t g g c a g g a c g t g g t c c t g a

3060 WLNGKEYKCKVSNKALPAPI g g c t g a a t g g c a a g g a g t a c a a g t g c a a g g t c t c c a a c a a a g c c c t c c c a g c c c c c a t c g c c g a c t t a c c g t t c c t c a t g t t c a c g t t c c a g a g g t t g t t t c g g g a g g g t c g g g g g t a g c

3120 EKTISKAKGQPREPQVYTLP a g a a a a c c a t c t c c a a a g c c a a a g g g c a g c c c c g a g a a c c a c a g g t g t a c a c c c t g c c c c t c t t t t g g t a g a g g t t t c g g t t t c c c g t c g g g g c t c t t g g t g t c c a c a t g t g g g a c g g g g

3180 PSREEMTKNQVSLTCLVKGF c a t c c c g g g a g g a g a t g a c c a a g a a c c a g g t c a g c c t g a c c t g c c t g g t c a a a g g c t t c t g t a g g g c c c t c c t c t a c t g g t t c t t g g t c c a g t c g g a c t g g a c g g a c c a g t t t c c g a a g a

3240 YPSDIAVEWESNGQPENNYK a t c c c a g c g a c a t c g c c g t g g a g t g g g a g a g c a a t g g g c a g c c g g a g a a c a a c t a c a a g a t a g g g t c g c t g t a g c g g c a c c t c a c c c t c t c g t t a c c c g t c g g c c t c t t g t t g a t g t t c t

3300 TTPPVLDSDGSFFLYSKLTV c c a c g c c t c c c g t g c t g g a c t c c g a c g g c t c c t t c t t c c t c t a c a g c a a g c t c a c c g t g g g g t g c g g a g g g c a c g a c c t g a g g c t g c c g a g g a a g a a g g a g a t g t c g t t c g a g t g g c a c c

3360 DKSRWQQGNVFSCSVMHEAL a c a a g a g c a g g t g g c a g c a g g g g a a c g t c t t c t c a t g c t c c g t g a t g c a c g a g g c t c t g c t g t t c t c g t c c a c c g t c g t c c c c t t g c a g a a g a g t a c g a g g c a c t a c g t g c t c c g a g a c g

BmtINheI 3420 HNHYTQKSLSLSPGK* a c a a c c a c t a c a c g c a g a a g a g c c t c t c c c t g t c t c c g g g t a a a t g a g t g c t a g c t g g c c t g t t g g t g a t g t g c g t c t t c t c g g a g a g g g a c a g a g g c c c a t t t a c t c a c g a t c g a c c g g

3480 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t

3540 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t

3600 SV40 poly(A) signal t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c

3660 β- globin poly(A) g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a t g g a a t t a a t t c t a a a a t a c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t a c c t t a a t t a a g a t t t t a t

3720 β- globin poly(A) c a g c a t a g c a a a a c t t t a a c c t c c a a a t c a a g c c t c t a c t t g a a t c c t t t t c t g a g g g a t g t c g t a t c g t t t t g a a a t t g g a g g t t t a g t t c g g a g a t g a a c t t a g g a a a a g a c t c c c t a

3780 β- globin poly(A) g a a t a a g g c a t a g g c a t c a g g g g c t g t t g c c a a t g t g c a t t a g c t g t t t g c a g c c t c a c c c t t a t t c c g t a t c c g t a g t c c c c g a c a a c g g t t a c a c g t a a t c g a c a a a c g t c g g a g t g g

3840 β- globin poly(A) t t c t t t c a t g g a g t t t a a g a t a t a g t g t a t t t t c c c a a g g t t t g a a c t a g c t c t t c a t t t a a g a a a g t a c c t c a a a t t c t a t a t c a c a t a a a a g g g t t c c a a a c t t g a t c g a g a a g t a a a

SspI 3900 β- globin poly(A) c t t t a t g t t t t a a a t g c a c t g a c c t c c c a c a t t c c c t t t t t a g t a a a a t a t t c a g a a a t a g a a a t a c a a a a t t t a c g t g a c t g g a g g g t g t a a g g g a a a a a t c a t t t t a t a a g t c t t t a t

3960 β- globin poly(A) a t t t a a a t a c a t c a t t g c a a t g a a a a t a a a t g t t t t t t a t t a g g c a g a a t c c a g a t g c t c t a a a t t t a t g t a g t a a c g t t a c t t t t a t t t a c a a a a a a t a a t c c g t c t t a g g t c t a c g a g

4020 β- globin poly(A) a a g g c c c t t c a t a a t a t c c c c c a g t t t a g t a g t t g g a c t t a g g g a a c a a a g g a a c c t t t a t t c c g g g a a g t a t t a t a g g g g g t c a a a t c a t c a a c c t g a a t c c c t t g t t t c c t t g g a a a t

4080 125 120 *DQEEA β- globin poly(A) BleoR a t a g a a a t t g g a c a g c a a g a a a g c g a g c t t c t a g c t t a t c c t c a g t c c t g c t c c t c t g c c t a t c t t t a a c c t g t c g t t c t t t c g c t c g a a g a t c g a a t a g g a g t c a g g a c g a g g a g a c g g

DraIII 4140 115 110 105 100 VFHVCNGAPDRLAFERGWPQ BleoR a c a a a g t g c a c g c a g t t g c c g g c c g g g t c g c g c a g g g c g a a c t c c c g c c c c c a c g g c t g c t g t t t c a c g t g c g t c a a c g g c c g g c c c a g c g c g t c c c g c t t g a g g g c g g g g g t g c c g a c g

FseI 4200 95 90 85 80 EGIETMAPGSADRFNTSVVE BleoR t c g c c g a t c t c g g t c a t g g c c g g c c c g g a g g c g t c c c g g a a g t t c g t g g a c a c g a c c t c c a g c g g c t a g a g c c a g t a c c g g c c g g g c c t c c g c a g g g c c t t c a a g c a c c t g t g c t g g a g g

4260 75 70 65 60 SWEAYLEDLGRVWVWALTND BleoR g a c c a c t c g g c g t a c a g c t c g t c c a g g c c g c g c a c c c a c a c c c a g g c c a g g g t g t t g t c c c t g g t g a g c c g c a t g t c g a g c a g g t c c g g c g c g t g g g t g t g g g t c c g g t c c c a c a a c a g g

4320 55 50 45 40 PVVQDQVASIFLTVDDRVVG BleoR g g c a c c a c c t g g t c c t g g a c c g c g c t g a t g a a c a g g g t c a c g t c g t c c c g g a c c a c a c c g c c g t g g t g g a c c a g g a c c t g g c g c g a c t a c t t g t c c c a g t g c a g c a g g g c c t g g t g t g g c

4380 35 30 25 20 AFDDEVFDRSFGLRDTWFEV BleoR g c g a a g t c g t c c t c c a c g a a g t c c c g g g a g a a c c c g a g c c g g t c g g t c c a g a a c t c g a c c c g c t t c a g c a g g a g g t g c t t c a g g g c c c t c t t g g g c t c g g c c a g c c a g g t c t t g a g c t g g

MauBI 4440 15 10 5 1 AGAVDRATLVPVASTLKAM BleoR g c t c c g g c g a c g t c g c g c g c g g t g a g c a c c g g a a c g g c a c t g g t c a a c t t g g c c a t g a t g c g a g g c c g c t g c a g c g c g c g c c a c t c g t g g c c t t g c c g t g a c c a g t t g a a c c g g t a c t a c

4500 g c t c c t c c t g t c a g g a g a g g a a a g a g a a g a a g g t t a g t a c a a t t g c t a t a g t g a g t t g t a c g a g g a g g a c a g t c c t c t c c t t t c t c t t c t t c c a a t c a t g t t a a c g a t a t c a c t c a a c a t

4560 t t a t a c t a t g c a g a t a t a c t a t g c c a a t g a t t a a t t g t c a a a c t a g g g c t g c a g g g t t c a a a t a t g a t a c g t c t a t a t g a t a c g g t t a c t a a t t a a c a g t t t g a t c c c g a c g t c c c a a g t

4620 t a g t g c c a c t t t t c c t g c a c t g c c c c a t c t c c t g c c c a c c c t t t c c c a g g c a t a g a c a g t a t c a c g g t g a a a a g g a c g t g a c g g g g t a g a g g a c g g g t g g g a a a g g g t c c g t a t c t g t c a

4680 c a g t g a c t t a c c a a a c t c a c a g g a g g g a g a a g g c a g a a g c t t g a g a c a g a c c c g c g g g a c g t c a c t g a a t g g t t t g a g t g t c c t c c c t c t t c c g t c t t c g a a c t c t g t c t g g g c g c c c t g

4740 c g c c g a a c t g c g a g g g g a c g t g g c t a g g g c g g c t t c t t t t a t g g t g c g c c g g c c c t c g g a g c g g c t t g a c g c t c c c c t g c a c c g a t c c c g c c g a a g a a a a t a c c a c g c g g c c g g g a g c c t

4800 g g c a g g g c g c t c g g g g a g g c c t a g c g g c c a a t c t g c g g t g g c a g g a g g c g g g g c c g a a g g c c g t c c c g c g a g c c c c t c c g g a t c g c c g g t t a g a c g c c a c c g t c c t c c g c c c c g g c t t c c

4860 c c g t g c c t g a c c a a t c c g g a g c a c a t a g g a g t c t c a g c c c c c c g c c c c a a a g c a a g g g g a g g c a c g g a c t g g t t a g g c c t c g t g t a t c c t c a g a g t c g g g g g g c g g g g t t t c g t t c c c c t

PspOMIApaI 4920 a g t c a c g c g c c t g t a g c g c c a g c g t g t t g t g a a a t g g g g g c t t g g g g g g g t t g g g g c c c t t c a g t g c g c g g a c a t c g c g g t c g c a c a a c a c t t t a c c c c c g a a c c c c c c c a a c c c c g g g a

SpeI 4980 g a c t a g t c a a a a c a a a c t c c c a t t g a c g t c a a t g g g g t g g a g a c t t g g a a a t c c c c g t g a c t g a t c a g t t t t g t t t g a g g g t a a c t g c a g t t a c c c c a c c t c t g a a c c t t t a g g g g c a c t

5040 CMV enhancer g t c a a a c c g c t a t c c a c g c c c a t t g a t g t a c t g c c a a a a c c g c a t c a t c a t g g t a a t a g c c a g t t t g g c g a t a g g t g c g g g t a a c t a c a t g a c g g t t t t g g c g t a g t a g t a c c a t t a t c g

SnaBI 5100 CMV enhancer g a t g a c t a a t a c g t a g a t g t a c t g c c a a g t a g g a a a g t c c c a t a a g g t c a t g t a c t g g g c c t a c t g a t t a t g c a t c t a c a t g a c g g t t c a t c c t t t c a g g g t a t t c c a g t a c a t g a c c c g

5160 CMV enhancer a t a a t g c c a g g c g g g c c a t t t a c c g t c a t t g a c g t c a a t a g g g g g c g t a c t t g g c a t a t g t a t t a c g g t c c g c c c g g t a a a t g g c a g t a a c t g c a g t t a t c c c c c g c a t g a a c c g t a t a c

5220 CMV enhancer a t a c a c t t g a t g t a c t g c c a a g t g g g c a g t t t a c c g t a a a t a c t c c a c c c a t t g a c g t c a t a t g t g a a c t a c a t g a c g g t t c a c c c g t c a a a t g g c a t t t a t g a g g t g g g t a a c t g c a g t

5280 CMV enhancer a t g g a a a g t c c c t a t t g g c g t t a c t a t g g g a a c a t a c g t c a t t a t t g a c g t c a a t g g g c g t a c c t t t c a g g g a t a a c c g c a a t g a t a c c c t t g t a t g c a g t a a t a a c t g c a g t t a c c c g c

5340 CMV enhancer g g g g t c g t t g g g c g g t c a g c c a g g c g g g c c a t t t a c c g t a a g t t a t g t a a c g c c t g c a g g c c c c a g c a a c c c g c c a g t c g g t c c g c c c g g t a a a t g g c a t t c a a t a c a t t g c g g a c g t c c

AflIIIPciI 5400 t t a a t t a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g a a t t a a t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c

5460 ori t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t

5520 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g

5580 ori t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g

5640 ori c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c

5700 ori g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g

5760 ori t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t

5820 ori g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c

5880 ori a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c

5940 ori a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a

6000 ori g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t

6060 g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t

6120 poly(A) signal g g g a t t t t g g t c a t g g c t a g t t a a t t a a c a t t t a a a t c a g c g g c c g c a a t a a a a t a t c t t c c c t a a a a c c a g t a c c g a t c a a t t a a t t g t a a a t t t a g t c g c c g g c g t t a t t t t a t a g a a

6180 poly(A) signal pause site t a t t t t c a t t a c a t c t g t g t g t t g g t t t t t t g t g t g a a t c g t a a c t a a c a t a c g c t c t c c a t a a a a g t a a t g t a g a c a c a c a a c c a a a a a a c a c a c t t a g c a t t g a t t g t a t g c g a g a g g

6240 pause site C T A G C A A A A T A G G C T G T C C C RVprimer3 a t c a a a a c a a a a c g a a a c a a a a c a a a c t a g c a a a a t a g g c t g t c c c c a g t g c a a g t g c a g t a g t t t t g t t t t g c t t t g t t t t g t t t g a t c g t t t t a t c c g a c a g g g g t c a c g t t c a c g t c

6264 pause site g t g c c a g a a c a t t t c t c t a t c g a a c a c g g t c t t g t a a a g a g a t a g c t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.