Addgene FUW-hSNCA(D2E)-NE Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: FUW-hSNCA(D2E)-NE Sequencing Result


Map View

SgrDI (9668) SspI (9551) Amp-R (9297 .. 9316) ScaI (9227) PvuI (9117) FspI (8969) AhdI (8747) pBR322ori-F (7995 .. 8014) PciI (7854) L4440 (7744 .. 7761) CAP binding site lac promoter M13/pUC Reverse (7526 .. 7548) lac operator M13 Reverse (7513 .. 7529) M13 rev BstZ17I (7475) EBV-rev (7434 .. 7453) SV40pA-R (7380 .. 7399) FseI (7115) SgrAI (6953) MauBI (6875) MscI (6842) EM7 promoter SV40pro-F (6638 .. 6657) pBABE 3' (6391 .. 6411) F1ori-F (6251 .. 6272) F1ori-R (6041 .. 6060) BGH-rev (5678 .. 5695) PmeI (5662) ApaI (5657) PspOMI (5653) 5' LTR (truncated) BspEI (5459) KpnI (5085) Acc65I (5081) Bsu36I (5077) pBluescriptKS (4951 .. 4967) pRS-marker (26 .. 45) MluI (210) CMV enhancer NdeI (466) SnaBI (572) CMV-F (751 .. 771) NotI (1504) BbvCI (1780) AleI (1933) PacI (2577) AfeI (2656) PaqCI (3493) hUBCpro-F (3649 .. 3668) XbaI (3835) HpaI (4332) WPRE-R (4412 .. 4432) PflMI (4600) PaeR7I - XhoI (4963) FUW-hSNCA(D2E)-NE 9688 bp

Sequence View

5ʹ 3ʹ 60 A T G T T A G A C G A G A C T A C G G C pRS-marker c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a

120 a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t

180 c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c

MluI 240 CMV enhancer c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a g t t a t t g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t c a a t a a

300 CMV enhancer a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a

360 CMV enhancer a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t

420 CMV enhancer t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c

NdeI 480 CMV enhancer a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g

540 CMV enhancer c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a

SnaBI 600 CMV enhancer CMV promoter t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t

660 CMV promoter t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t

720 CMV promoter g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g

780 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c

840 CMV promoter 5' LTR (truncated) a g g t c t a t a t a a g c a g c g c g t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a t c c a g a t a t a t t c g t c g c g c a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t

900 5' LTR (truncated) g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a

960 5' LTR (truncated) t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g

1020 5' LTR (truncated) a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c

1080 HIV-1 Ψ c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c

1140 HIV-1 Ψ c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t

1200 HIV-1 Ψ a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c

1260 a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c

1320 c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a

1380 g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a

1440 c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t

1500 c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g

NotI 1560 MRDNWRSE a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t

1620 LYKYKVVKIEPLGVAPTKAK t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c

1680 RRE RRVVQREKRAVGIGALFLGF a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g

1740 RRE LGAAGSTMGAASMTLTVQAR t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t

BbvCI 1800 RRE QLLSGIVQQQNNLLRAIEAQ c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t

1860 RRE QHLLQLTVWGIKQLQARILA c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a

1920 RRE VERYLKDQQLLGIWGCSGKL g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g

AleI 1980 ICTTAVPWNASWSNKSLEQI a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a

2040 WNHTTWMEWDREINNYTSLI t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t

2100 1 5 KNEQELLE gp41 peptide HSLIEESQNQQEKNEQELLE c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t

2160 10 15 LDKWASL (in frame with gp41 peptide) WNWFNITNWLWYI gp41 peptide LDKWASLWNWFNITNWLWYI t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t

2220 (in frame with gp41 peptide) KLFIMIVGGLVGLRIVFAVL KLFIMIVGGLVGLRIVFAVL a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a

2280 (in frame with gp41 peptide) SIVNRVRQGYSPLSFQTHLP SIVNRVRQGYSPLSFQTHLP t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t

2340 (in frame with gp41 peptide) TPRGPDRPEGIEEEGGERDR TPRGPDRPEGIEEEGGERDR a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g a t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c t

2400 (in frame with gp41 peptide) DRSIRLVNGSALRAPILQTN DRSIRLVNGSALRAPILQTN g a c a g a t c c a t t c g a t t a g t g a a c g g a t c g g c a c t g c g t g c g c c a a t t c t g c a g a c a a a t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g c c g t g a c g c a c g c g g t t a a g a c g t c t g t t t a

2460 (in frame with gp41 peptide) GSIHPQF* cPPT/CTS GSIHPQF* g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t

2520 cPPT/CTS a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g

PacI 2580 cPPT/CTS a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a g t t t g g t t a a t t a a t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t c a a a c c a a t t a a t t

2640 UbC promoter c c c g t g t c g g c t c c a g a t c t g g c c t c c g c g c c g g g t t t t g g c g c c t c c c g c g g g c g c c c c g g g c a c a g c c g a g g t c t a g a c c g g a g g c g c g g c c c a a a a c c g c g g a g g g c g c c c g c g g g g

AfeI 2700 UbC promoter c c t c c t c a c g g c g a g c g c t g c c a c g t c a g a c g a a g g g c g c a g c g a g c g t c c t g a t c c t t c g g a g g a g t g c c g c t c g c g a c g g t g c a g t c t g c t t c c c g c g t c g c t c g c a g g a c t a g g a a g

2760 UbC promoter c g c c c g g a c g c t c a g g a c a g c g g c c c g c t g c t c a t a a g a c t c g g c c t t a g a a c c c c a g t a g c g g g c c t g c g a g t c c t g t c g c c g g g c g a c g a g t a t t c t g a g c c g g a a t c t t g g g g t c a t

2820 UbC promoter t c a g c a g a a g g a c a t t t t a g g a c g g g a c t t g g g t g a c t c t a g g g c a c t g g t t t t c t t t c c a g t c g t c t t c c t g t a a a a t c c t g c c c t g a a c c c a c t g a g a t c c c g t g a c c a a a a g a a a g g

2880 UbC promoter a g a g a g c g g a a c a g g c g a g g a a a a g t a g t c c c t t c t c g g c g a t t c t g c g g a g g g a t c t c c t c t c t c g c c t t g t c c g c t c c t t t t c a t c a g g g a a g a g c c g c t a a g a c g c c t c c c t a g a g g

2940 UbC promoter g t g g g g c g g t g a a c g c c g a t g a t t a t a t a a g g a c g c g c c g g g t g t g g c a c a g c t a g t t c c c a c c c c g c c a c t t g c g g c t a c t a a t a t a t t c c t g c g c g g c c c a c a c c g t g t c g a t c a a g g

3000 UbC promoter g t c g c a g c c g g g a t t t g g g t c g c g g t t c t t g t t t g t g g a t c g c t g t g a t c g t c a c t t g g t c a g c g t c g g c c c t a a a c c c a g c g c c a a g a a c a a a c a c c t a g c g a c a c t a g c a g t g a a c c a

3060 UbC promoter g a g t a g c g g g c t g c t g g g c t g g c c g g g g c t t t c g t g g c c g c c g g g c c g c t c g g t g g g a c g c t c a t c g c c c g a c g a c c c g a c c g g c c c c g a a a g c a c c g g c g g c c c g g c g a g c c a c c c t g c

3120 UbC promoter g a a g c g t g t g g a g a g a c c g c c a a g g g c t g t a g t c t g g g t c c g c g a g c a a g g t t g c c c t g a c t t c g c a c a c c t c t c t g g c g g t t c c c g a c a t c a g a c c c a g g c g c t c g t t c c a a c g g g a c t

3180 UbC promoter a c t g g g g g t t g g g g g g a g c g c a g c a a a a t g g c g g c t g t t c c c g a g t c t t g a a t g g a a g a c t g a c c c c c a a c c c c c c t c g c g t c g t t t t a c c g c c g a c a a g g g c t c a g a a c t t a c c t t c t g

3240 UbC promoter g c t t g t g a g g c g g g c t g t g a g g t c g t t g a a a c a a g g t g g g g g g c a t g g t g g g c g g c a a g a c g a a c a c t c c g c c c g a c a c t c c a g c a a c t t t g t t c c a c c c c c c g t a c c a c c c g c c g t t c t

3300 UbC promoter a c c c a a g g t c t t g a g g c c t t c g c t a a t g c g g g a a a g c t c t t a t t c g g g t g a g a t g g g c t g t g g g t t c c a g a a c t c c g g a a g c g a t t a c g c c c t t t c g a g a a t a a g c c c a c t c t a c c c g a c

3360 UbC promoter g g g c a c c a t c t g g g g a c c c t g a c g t g a a g t t t g t c a c t g a c t g g a g a a c t c g g t t t g t c g c c c g t g g t a g a c c c c t g g g a c t g c a c t t c a a a c a g t g a c t g a c c t c t t g a g c c a a a c a g c

3420 UbC promoter t c t g t t g c g g g g g c g g c a g t t a t g g c g g t g c c g t t g g g c a g t g c a c c c g t a c c t t t g g g a a g a c a a c g c c c c c g c c g t c a a t a c c g c c a c g g c a a c c c g t c a c g t g g g c a t g g a a a c c c t

3480 UbC promoter g c g c g c g c c c t c g t c g t g t c g t g a c g t c a c c c g t t c t g t t g g c t t a t a a t g c a g g g t g g g c g c g c g c g g g a g c a g c a c a g c a c t g c a g t g g g c a a g a c a a c c g a a t a t t a c g t c c c a c c c

PaqCI 3540 UbC promoter g c c a c c t g c c g g t a g g t g t g c g g t a g g c t t t t c t c c g t c g c a g g a c g c a g g g t t c g g g c c c g g t g g a c g g c c a t c c a c a c g c c a t c c g a a a a g a g g c a g c g t c c t g c g t c c c a a g c c c g g

3600 UbC promoter t a g g g t a g g c t c t c c t g a a t c g a c a g g c g c c g g a c c t c t g g t g a g g g g a g g g a t a a g t g a a t c c c a t c c g a g a g g a c t t a g c t g t c c g c g g c c t g g a g a c c a c t c c c c t c c c t a t t c a c t

3660 UbC promoter T G A A G C T C C G G T hUBCpro-F g g c g t c a g t t t c t t t g g t c g g t t t t a t g t a c c t a t c t t c t t a a g t a g c t g a a g c t c c g g t c c g c a g t c a a a g a a a c c a g c c a a a a t a c a t g g a t a g a a g a a t t c a t c g a c t t c g a g g c c a

3720 UbC promoter T T T G A A C T hUBCpro-F t t t g a a c t a t g c g c t c g g g g t t g g c g a g t g t g t t t t g t g a a g t t t t t t a g g c a c c t t t t g a a a c t t g a t a c g c g a g c c c c a a c c g c t c a c a c a a a a c a c t t c a a a a a a t c c g t g g a a a a c

3780 UbC promoter a a a t g t a a t c a t t t g g g t c a a t a t g t a a t t t t c a g t g t t a g a c t a g t a a a t t g t c c g c t a t t t a c a t t a g t a a a c c c a g t t a t a c a t t a a a a g t c a c a a t c t g a t c a t t t a a c a g g c g a t

XbaI 3840 UbC promoter a a t t c t g g c c g t t t t t g g c t t t t t t g t t a g a c g a a g c t t g g g c t g c a g g t c g a c t c t a g a t t a a g a c c g g c a a a a a c c g a a a a a a c a a t c t g c t t c g a a c c c g a c g t c c a g c t g a g a t c t

3900 Kozak sequence MEVFMKGLSKAKEGVVAA g c c a c c a t g g a g g t a t t c a t g a a a g g a c t t t c a a a g g c c a a g g a g g g a g t t g t g g c t g c t c g g t g g t a c c t c c a t a a g t a c t t t c c t g a a a g t t t c c g g t t c c t c c c t c a a c a c c g a c g a

3960 AEKTKQGVAEAAGKTKEGVL g c t g a g a a a a c c a a a c a g g g t g t g g c a g a a g c a g c a g g a a a g a c a a a a g a g g g t g t t c t c c g a c t c t t t t g g t t t g t c c c a c a c c g t c t t c g t c g t c c t t t c t g t t t t c t c c c a c a a g a g

4020 YVGSKTKEGVVHGVATVAEK t a t g t a g g c t c c a a a a c c a a g g a g g g a g t g g t g c a t g g t g t g g c a a c a g t g g c t g a g a a g a t a c a t c c g a g g t t t t g g t t c c t c c c t c a c c a c g t a c c a c a c c g t t g t c a c c g a c t c t t c

4080 TKEQVTNVGGAVVTGVTAVA a c c a a a g a g c a a g t g a c a a a t g t t g g a g g a g c a g t g g t g a c g g g t g t g a c a g c a g t a g c c t g g t t t c t c g t t c a c t g t t t a c a a c c t c c t c g t c a c c a c t g c c c a c a c t g t c g t c a t c g g

4140 QKTVEGAGSIAAATGFVKKD c a g a a g a c a g t g g a g g g a g c a g g g a g c a t t g c a g c a g c c a c t g g c t t t g t c a a a a a g g a c g t c t t c t g t c a c c t c c c t c g t c c c t c g t a a c g t c g t c g g t g a c c g a a a c a g t t t t t c c t g

4200 QLGKNEEGAPQEGILEDMPV c a g t t g g g c a a g a a t g a a g a a g g a g c c c c a c a g g a a g g g a t t c t g g a a g a t a t g c c t g t g g t c a a c c c g t t c t t a c t t c t t c c t c g g g g t g t c c t t c c c t a a g a c c t t c t a t a c g g a c a c

4260 DPDNEAYEMPSEEGYQDYEP g a t c c t g a c a a t g a g g c t t a t g a a a t g c c t t c t g a g g a a g g g t a t c a a g a c t a c g a a c c t c t a g g a c t g t t a c t c c g a a t a c t t t a c g g a a g a c t c c t t c c c a t a g t t c t g a t g c t t g g a

4320 EATKENPRSNQEESYDDNES g a a g c c a c c a a a g a a a a c c c g c g t a g c a a c c a g g a a g a a a g c t a t g a t g a t a a c g a a a g c c t t c g g t g g t t t c t t t t g g g c g c a t c g t t g g t c c t t c t t t c g a t a c t a c t a t t g c t t t c g

HpaI 4380 WPRE * t a a g g a t c c g t t a a c a a t t c g a t a t c a a g c t t a t c g a t a a t c a a c c t c t g g a t t a c a a a a a t t c c t a g g c a a t t g t t a a g c t a t a g t t c g a a t a g c t a t t a g t t g g a g a c c t a a t g t t t t

4440 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c

4500 WPRE MLLLPVWLSFSPP MPLYHAIASRMAFIFSS c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a

4560 WPRE CINPGCCLFMRSCGPLSGNV LYKSWLLSLYEELWPVVRQR t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c

PflMI 4620 WPRE AWCALCLLTQPPLVGALPPP GVVCTVFADATPTGWGIATT g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a

4680 WPRE VSSFPGLSLSPSLLPRRNSS CQLLSGTFAFPLPIATAELI g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c

4740 WPRE PPALPAAGQGLGCWALTIPW AACLARCWTGARLLGTDNSV c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c

4800 WPRE (in frame with Factor Xa site) *RGKRPQEGTNGGPN CCRGNHRPFLGCSPVLPPGF VLSGKSSSFPWLLACVATWI t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g

4860 WPRE (in frame with Factor Xa site) QAPRGEAVDR 1 RGEI Factor Xa site CAGRPSATSLRPSIQRTFLP LRGTSFCYVPSALNPADLPS t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g

4920 WPRE AACCRLCGLFRVFAFALRRV RGLLPALRPLPRLRLRPQTS g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g

PaeR7IXhoI 4980 WPRE KS primer C T A T G G C A G C T G G A G C T pBluescriptKS GSPFGPPPRIDTVDLET* RISLWAASPHRYRRPRDLEK g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g a t a c c g t c g a c c t c g a g a c c t a g a a a a a c c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c t a t g g c a g c t g g a g c t c t g g a t c t t t t t g

5040 HGAITSSNTAATNADCAWLE a t g g a g c a a t c a c a a g t a g c a a t a c a g c a g c t a c c a a t g c t g a t t g t g c c t g g c t a g a a g t a c c t c g t t a g t g t t c a t c g t t a t g t c g t c g a t g g t t a c g a c t a a c a c g g a c c g a t c t t c

Bsu36IAcc65IKpnI 5100 AQEEEEVGFPVTPQVPLRPM c a c a a g a g g a g g a g g a g g t g g g t t t t c c a g t c a c a c c t c a g g t a c c t t t a a g a c c a a t g a g t g t t c t c c t c c t c c t c c a c c c a a a a g g t c a g t g t g g a g t c c a t g g a a a t t c t g g t t a c t

5160 TYKAAVDLSHFLKEKGGLEG c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g

5220 LIHSQRRQDILDLWIYHTQG t a a t t c a c t c c c a a c g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a c a c a a g g c t a t t a a g t g a g g g t t g c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t g t g t t c c g a

5280 YFPDWQNYTPGPGIRYPLTF a c t t c c c t g a t t g g c a g a a c t a c a c a c c a g g g c c a g g g a t c a g a t a t c c a c t g a c c t t t g t g a a g g g a c t a a c c g t c t t g a t g t g t g g t c c c g g t c c c t a g t c t a t a g g t g a c t g g a a a c

5340 GWCYKLVPVEQEKVEEANEG g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c a a g a g a a g g t a g a a g a a g c c a a t g a a g g a g c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g t t c t c t t c c a t c t t c t t c g g t t a c t t c c t c

5400 ENTRLLHPVSLHGMDDPERE a g a a c a c c c g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g a g a g a g a a g t c t t g t g g g c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c t c t c t c t t c

BspEI 5460 VLEWRFDSRLAFHHMARELH t a t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c a t g g c c c g a g a g c t g c a t c a t a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g t a c c g g g c t c t c g a c g t a g

5520 5' LTR (truncated) PDCTGSLWLDQI* c g g a c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c g c c t g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g

5580 5' LTR (truncated) t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c

5640 5' LTR (truncated) c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t

PmeIPspOMIApaI 5700 5' LTR (truncated) bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev a a a t c t c t a g c a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c t t t a g a g a t c g t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g

5760 bGH poly(A) signal c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g

5820 bGH poly(A) signal a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a

5880 bGH poly(A) signal a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c

5940 bGH poly(A) signal c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a

6000 f1 ori a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c

6060 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a

6120 f1 ori t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t

6180 f1 ori g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a

6240 f1 ori t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c

6300 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a

6360 f1 ori t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a

6420 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a

6480 SV40 promoter c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t

6540 SV40 promoter g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a

6600 SV40 promoter SV40 ori a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a

6660 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c

6720 SV40 promoter SV40 ori c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a

6780 SV40 promoter EM7 promoter g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c a c g t g t t g a c a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c g t g c a c a a c t g t t

6840 1 M EM7 promoter BleoR t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g g a a c t a a a c c a t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t c c t t g a t t t g g t a

MauBIMscI 6900 5 10 15 20 AKLTSAVPVLTARDVAGAVE BleoR g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t c a c c g c g c g c g a c g t c g c c g g a g c g g t c g a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a g t g g c g c g c g c t g c a g c g g c c t c g c c a g c t

SgrAI 6960 25 30 35 40 FWTDRLGFSRDFVEDDFAGV BleoR g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g g a c t t c g t g g a g g a c g a c t t c g c c g g t g t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c c c t g a a g c a c c t c c t g c t g a a g c g g c c a c a

7020 45 50 55 60 VRDDVTLFISAVQDQVVPDN BleoR g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c g c g g t c c a g g a c c a g g t g g t g c c g g a c a a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c g c g c c a g g t c c t g g t c c a c c a c g g c c t g t t

7080 65 70 75 80 TLAWVWVRGLDELYAEWSEV BleoR c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g g a c g a g c t g t a c g c c g a g t g g t c g g a g g t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a c c t g c t c g a c a t g c g g c t c a c c a g c c t c c a

FseI 7140 85 90 95 100 VSTNFRDASGPAMTEIGEQP BleoR c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g g c c g g c c a t g a c c g a g a t c g g c g a g c a g c c g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c c g g c c g g t a c t g g c t c t a g c c g c t c g t c g g

7200 105 110 115 120 WGREFALRDPAGNCVHFVAE BleoR g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c g g c c g g c a a c t g c g t g c a c t t c g t g g c c g a c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g c c g g c c g t t g a c g c a c g t g a a g c a c c g g c t

7260 125 EQD* BleoR g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a

7320 g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a

7380 SV40 poly(A) signal C SV40pA-R g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c

7440 SV40 poly(A) signal G T G G T T T EBV-rev G T T A T C G T A G T G T T T A A A G SV40pA-R c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a

BstZ17I 7500 SV40 poly(A) signal G T C C A A A C T C A T C EBV-rev g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a

7560 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t

7620 -10 -35 lac promoter CAP binding site c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a

7680 CAP binding site c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a

7740 g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g

7800 G A G C G A G T G A C T G A G C G A L4440 t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t

PciI 7860 c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c

7920 ori a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t

7980 ori t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t

8040 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g

8100 ori t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g

8160 ori g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a

8220 ori g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c

8280 ori t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c

8340 ori g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t

8400 ori c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c

8460 ori a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a

8520 ori t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a

8580 t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c

8640 a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a

8700 285 280 *WHKILSAG AmpR *WHKILSAG c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g

AhdI 8760 275 270 265 260 IEAIQRNREDMTAQSGTTYI AmpR IEAIQRNREDMTAQSGTTYI t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a

8820 255 250 245 240 VVIRSPKGDPGLAAIIGRSG AmpR VVIRSPKGDPGLAAIIGRSG a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g

8880 235 230 225 220 REGAGSKDAIFWGAPLASRL AmpR REGAGSKDAIFWGAPLASRL a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c

8940 215 210 205 200 LPGAVKDAEMWDILQQRSAL AmpR LPGAVKDAEMWDILQQRSAL a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c

FspI 9000 195 190 185 180 TLLEGTLLKRLTTAMAVPMT AmpR TLLEGTLLKRLTTAMAVPMT a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a

9060 175 170 165 160 TDREDNPIAENLEPEWRDLR AmpR TDREDNPIAENLEPEWRDLR g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c

PvuI 9120 155 150 145 140 TVHDGMNHLFATLEKPGGIT AmpR TVHDGMNHLFATLEKPGGIT a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a

9180 135 130 125 120 TLLLNAATNDSMTIAASCLE AmpR TLLLNAATNDSMTIAASCLE t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g

ScaI 9240 115 110 105 100 RVTMGDTLHKETVPSYEVLD AmpR RVTMGDTLHKETVPSYEVLD t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g

9300 95 90 85 80 NQSYHIRRGLQEQGADIRSL AmpR A T A A Amp-R NQSYHIRRGLQEQGADIRSL a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t

9360 75 70 65 60 VAGCLLVKFTSMMPFREEPR AmpR T A C C G C G C C A C A T A G C Amp-R VAGCLLVKFTSMMPFREEPR t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c

9420 55 50 45 40 FSELIKGSNLDLEIYGVRAG AmpR FSELIKGSNLDLEIYGVRAG a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c t a t g t a a c c c a c t c g t g c a c c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g a t a c a t t g g g t g a g c a c g t g g

9480 35 30 25 LQDEADKVKVLTEPH 20 signal sequence AFVPL AmpR LQDEADKVKVLTEPHAFVPL c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c

9540 15 10 5 1 signal sequence CFAAFFPILAVRFHQISM AmpR AmpR promoter CFAAFFPILAVRFHQISM g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a

SspI 9600 AmpR promoter c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a

9660 AmpR promoter t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g

SgrDI 3ʹ 5ʹ 9688 a c c t g a c g t c g a c g g a t c g g g a g a t c t c t g g a c t g c a g c t g c c t a g c c c t c t a g a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.