Addgene pEGFP-PRKCZ-C20 (N-PRKCZ-C20) - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pEGFP-PRKCZ-C20 (N-PRKCZ-C20)


Map View

2000 1500 1000 500 End (2069) EBV-rev (2039 .. 2058) SV40pA-R (1985 .. 2004) ApoI (1998) PsiI (1967) TsoI (1961) HpaI - HincII (1947) MfeI (1934) BsaBI * (1846) BclI * (1841) XbaI * (1831) SmaI (1817) ApaI (1816) XmaI - TspMI (1815) PspOMI (1812) SacII (1811) KpnI (1808) Acc65I (1804) BstBI (1754) PvuII (1704) PshAI (1658) MluI - AflIII (1574) AhdI (1566) PflMI (1549) NaeI (1540) NgoMIV (1538) PpuMI - EcoO109I (1515) PspFI (1342) BseYI (1338) BstAPI (1262) SmlI XhoI PaeR7I (1208) BglII (1204) BsrGI (1187) EGFP-C (1131 .. 1152) EXFP-R (784 .. 803) BssSI - BssSαI (658) EGFP-N (523 .. 544) BsiEI (470) AgeI (465) AfeI (461) BmtI (460) NheI (456) CMV-F (384 .. 404) BsaAI - SnaBI (205) NdeI (99) Start (0) CMV promoter EGFP SV40 poly(A) signal EGFP Kozak sequence pEGFP-PRKCZ-C20 (N-PRKCZ-C20) 2069 bp

Sequence View

Start (0) 60 g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a

NdeI 120 a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a

180 t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t

SnaBIBsaAI 240 CMV promoter c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a

300 CMV promoter t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t

360 CMV promoter c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c

420 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t

BsiEIAgeINheIBmtIAfeI 480 1 M CMV promoter EGFP 1 M EGFP Kozak sequence M *RDGGH t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a c c g g t c g c c a c c a t g a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t g g c c a g c g g t g g t a c

540 1a V 5 10 15 20 SKGEELFTGVVPILVELDG EGFP 1a V 5 10 15 20 SKGEELFTGVVPILVELDG EGFP Kozak sequence G A C C A G C T C G A C C T G C C G EGFP-N VSKGEELFTGVVPILVELDG HALALLQEGPHHGDQDLQVA g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g

600 25 30 35 40 DVNGHKFSVSGEGEGDATYG EGFP 25 30 35 40 DVNGHKFSVSGEGEGDATYG EGFP C T G C EGFP-N DVNGHKFSVSGEGEGDATYG VYVAVLEAHGALALAIGGVA g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g

BssSαIBssSI 660 45 50 55 60 KLTLKFICTTGKLPVPWPTL EGFP 45 50 55 60 KLTLKFICTTGKLPVPWPTL EGFP KLTLKFICTTGKLPVPWPTL LQGQLEDAGGALQGHGPGGE a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g

720 65 70 75 80 VTTLTYGVQCFSRYPDHMKQ EGFP 65 70 75 80 VTTLTYGVQCFSRYPDHMKQ EGFP VTTLTYGVQCFSRYPDHMKQ HGGQGVAHLAEAAVGVVHLL g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c

780 85 90 95 100 HDFFKSAMPEGYVQERTIFF EGFP 85 90 95 100 HDFFKSAMPEGYVQERTIFF EGFP HDFFKSAMPEGYVQERTIFF VVEELGGHGFAVDLLAGDEE c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g

840 105 110 115 120 KDDGNYKTRAEVKFEGDTLV EGFP 105 110 115 120 KDDGNYKTRAEVKFEGDTLV EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R KDDGNYKTRAEVKFEGDTLV LVVAVVLGAGLHLELAVGQH a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c

900 125 130 135 140 NRIELKGIDFKEDGNILGHK EGFP 125 130 135 140 NRIELKGIDFKEDGNILGHK EGFP NRIELKGIDFKEDGNILGHK VADLQLADVELLVAVDQPVL a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c

960 145 150 155 160 LEYNYNSHNVYIMADKQKNG EGFP 145 150 155 160 LEYNYNSHNVYIMADKQKNG EGFP LEYNYNSHNVYIMADKQKNG QLVVVVAVVDIDHGVLLLVA c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g

1020 165 170 175 180 IKVNFKIRHNIEDGSVQLAD EGFP 165 170 175 180 IKVNFKIRHNIEDGSVQLAD EGFP IKVNFKIRHNIEDGSVQLAD DLHVELDAVVDLVAAHLEGV a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g

1080 185 190 195 200 HYQQNTPIGDGPVLLPDNHY EGFP 185 190 195 200 HYQQNTPIGDGPVLLPDNHY EGFP HYQQNTPIGDGPVLLPDNHY VVLLVGGDAVAGHQQGVVVV c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g

1140 205 210 215 220 LSTQSALSKDPNEKRDHMVL EGFP 205 210 215 220 LSTQSALSKDPNEKRDHMVL EGFP C A T G G T C C T G EGFP-C LSTQSALSKDPNEKRDHMVL QAGLGGQAFVGVLLAIVHDQ c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c

BsrGI 1200 225 230 235 LEFVTAAGITLGMDELYK SG EGFP 225 230 235 LEFVTAAGITLGMDELYK SG EGFP C T G G A G T T C G T G EGFP-C LEFVTAAGITLGMDELYKSG QLEHGGGPDSEAHVLQVLGS c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t c c g g a g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a g g c c t

BglIIPaeR7IXhoISmlI 1260 (in frame with EGFP) LRSRAGGSSGGGGAGDTTST (in frame with EGFP) LRSRAGGSSGGGGAGDTTST LRSRAGGSSGGGGAGDTTST ESRSSATGRTTTTRTVCRAS c t c a g a t c t c g a g c t g g c g g t t c c t c t g g t g g t g g t g g t g c g g g t g a c a c a a c g a g c a c t g a g t c t a g a g c t c g a c c g c c a a g g a g a c c a c c a c c a c c a c g c c c a c t g t g t t g c t c g t g a

BstAPI 1320 (in frame with EGFP) FCGTPNYIAPEILRGEEYGF (in frame with EGFP) FCGTPNYIAPEILRGEEYGF FCGTPNYIAPEILRGEEYGF EASGRIVDGGFDQPSLLVPE t t c t g c g g a a c c c c g a a t t a c a t c g c c c c c g a a a t c c t g c g g g g a g a g g a g t a c g g g t t c a a g a c g c c t t g g g g c t t a a t g t a g c g g g g g c t t t a g g a c g c c c c t c t c c t c a t g c c c a a g

BseYIPspFI 1380 (in frame with EGFP) SVDWWALGVLMFEMMAGRSP (in frame with EGFP) SVDWWALGVLMFEMMAGRSP SVDWWALGVLMFEMMAGRSP AHVPPRQSDEHKLHHGPAGR a g c g t g g a c t g g t g g g c g c t g g g a g t c c t c a t g t t t g a g a t g a t g g c c g g g c g c t c c c c g t c g c a c c t g a c c a c c c g c g a c c c t c a g g a g t a c a a a c t c t a c t a c c g g c c c g c g a g g g g c

1440 (in frame with EGFP) FDIITDNPDMNTEDYLFQVI (in frame with EGFP) FDIITDNPDMNTEDYLFQVI FDIITDNPDMNTEDYLFQVI EVDDGVVRVHVCLVVKELHD t t c g a c a t c a t c a c c g a c a a c c c g g a c a t g a a c a c a g a g g a c t a c c t t t t c c a a g t g a t c a a g c t g t a g t a g t g g c t g t t g g g c c t g t a c t t g t g t c t c c t g a t g g a a a a g g t t c a c t a g

1500 (in frame with EGFP) LEKPIRIPRFLSVKASHVLK (in frame with EGFP) LEKPIRIPRFLSVKASHVLK LEKPIRIPRFLSVKASHVLK QLLGDPDGPEQGDFGGM c t g g a g a a g c c c a t c c g g a t c c c c c g g t t c c t g t c c g t c a a a g c c t c c c a t g t t t t a a a a g a c c t c t t c g g g t a g g c c t a g g g g g c c a a g g a c a g g c a g t t t c g g a g g g t a c a a a a t t t t

PflMINgoMIVNaeIEcoO109IPpuMI 1560 (in frame with EGFP) GFLNKDPKERLGCRPQTGFS (in frame with EGFP) GFLNKDPKERLGCRPQTGFS GFLNKDPKERLGCRPQTGFS g g a t t t t t a a a t a a g g a c c c c a a a g a g a g g c t c g g c t g c c g g c c a c a g a c t g g a t t t t c t c c t a a a a a t t t a t t c c t g g g g t t t c t c t c c g a g c c g a c g g c c g g t g t c t g a c c t a a a a g a

AflIIIMluIAhdI 1620 (in frame with EGFP) DIKSHAFFRSIDWDLLEKKQ (in frame with EGFP) DIKSHAFFRSIDWDLLEKKQ DIKSHAFFRSIDWDLLEKKQ g a c a t c a a g t c c c a c g c g t t c t t c c g c a g c a t a g a c t g g g a c t t g c t g g a g a a g a a g c a g c t g t a g t t c a g g g t g c g c a a g a a g g c g t c g t a t c t g a c c c t g a a c g a c c t c t t c t t c g t c

PshAI 1680 (in frame with EGFP) ALPPFQPQITDDYGLDNFDT (in frame with EGFP) ALPPFQPQITDDYGLDNFDT ALPPFQPQITDDYGLDNFDT g c g c t c c c t c c a t t c c a g c c a c a g a t c a c a g a c g a c t a c g g t c t g g a c a a c t t t g a c a c a c g c g a g g g a g g t a a g g t c g g t g t c t a g t g t c t g c t g a t g c c a g a c c t g t t g a a a c t g t g t

PvuII 1740 (in frame with EGFP) QFTSEPVQLTPDDEDAIKRI (in frame with EGFP) QFTSEPVQLTPDDEDAIKRI QFTSEPVQLTPDDEDAIKRI c a g t t c a c c a g c g a g c c c g t g c a g c t g a c c c c a g a c g a t g a g g a t g c c a t a a a g a g g a t c g t c a a g t g g t c g c t c g g g c a c g t c g a c t g g g g t c t g c t a c t c c t a c g g t a t t t c t c c t a g

BstBI 1800 (in frame with EGFP) DQSEFEGFEYINPLLLSTEE (in frame with EGFP) DQSEFEGFEYINPLLLSTEE DQSEFEGFEYINPLLLSTEE g a c c a g t c a g a g t t c g a a g g c t t t g a g t a t a t c a a c c c a t t a t t g c t g t c c a c c g a g g a g c t g g t c a g t c t c a a g c t t c c g a a a c t c a t a t a g t t g g g t a a t a a c g a c a g g t g g c t c c t c

BsaBI*BclI*XbaI*Acc65IKpnISacIIPspOMITspMIXmaIApaISmaI 1860 (in frame with EGFP) SGTAGPGSTGSR* (in frame with EGFP) SGTAGPGSTGSR* SGTAGPGSTGSR* t c g g g t a c c g c g g g c c c g g g a t c c a c c g g a t c t a g a t a a c t g a t c a t a a t c a g c c a t a c c a g c c c a t g g c g c c c g g g c c c t a g g t g g c c t a g a t c t a t t g a c t a g t a t t a g t c g g t a t g g

1920 a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t

PsiITsoIHincIIHpaIMfeI 1980 SV40 poly(A) signal c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t

ApoI 2040 SV40 poly(A) signal G T EBV-rev C G T T A T C G T A G T G T T T A A A G SV40pA-R t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a

End (2069) 2069 SV40 poly(A) signal G G T T T G T C C A A A C T C A T C EBV-rev g g t t t g t c c a a a c t c a t c a a t g t a t c t t a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.