Addgene pTST-EGFP-GPIBY55 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pTST-EGFP-GPIBY55 Sequencing Result


Map View

PciI (4966) pBR322ori-F (4811 .. 4830) ApaLI (4652) BsaI (4037) TK-pA-R (3902 .. 3921) Neo-F (3584 .. 3603) RsrII (3564) BsrDI (3281) PflFI - Tth111I (3166) FspI (3150) PluTI (3051) SfoI (3049) NarI (3048) KasI (3047) Neo-R (2974 .. 2993) BspDI * - ClaI * (2888) StuI (2869) SfiI (2823) SV40 promoter CsiI - SexAI * (2637) pBABE 3' (2551 .. 2571) AseI (7) CMV enhancer NdeI (234) SnaBI (340) CMV-F (519 .. 539) NheI (591) BmtI (595) AfeI (596) AgeI (600) EcoNI (625) EGFP-N (831 .. 852) EXFP-R (1092 .. 1111) EGFP-C (1439 .. 1460) BsrGI (1495) BstEII (1598) BglII (1632) PaeR7I - XhoI (1636) Eco53kI (1641) SacI (1643) HindIII (1645) EcoRI (1652) PstI (1661) SalI (1662) AccI (1663) SacII (1675) PspOMI (1676) TspMI - XmaI (1679) ApaI (1680) SmaI (1681) BamHI (1683) XbaI * (1695) BclI * (1705) MfeI (1798) HpaI (1811) SV40pA-R (1849 .. 1868) EBV-rev (1903 .. 1922) MluI (1934) F1ori-F (2077 .. 2098) DraIII (2164) F1ori-R (2289 .. 2308) pTST-EGFP-GPIBY55 5024 bp

Sequence View

AseI 5ʹ 3ʹ 60 t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c

120 CMV enhancer c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a

180 CMV enhancer g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t

NdeI 240 CMV enhancer a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g

300 CMV enhancer a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t

SnaBI 360 CMV enhancer c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g

420 CMV enhancer CMV promoter c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c

480 CMV promoter *HSNTKAGFDV a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F PSELIDYCSRGMSAFPRYAH g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a

NheIBmtIAfeI 600 CMV promoter VTPPRYLCLQNLSGDSGSAS a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t

EcoNIAgeI 660 MLLEPGRGCCALAILLA GTGHQQLRASAAASQGDQQG c c g g t a c c a t g c t g c t g g a g c c t g g c a g a g g c t g c t g c g c t c t g g c c a t c c t g c t g g c c a g g c c a t g g t a c g a c g a c c t c g g a c c g t c t c c g a c g a c g c g a g a c c g g t a g g a c g a c c g g t

720 1 SA 5 10 Strep-Tag II WSHPQFEK GG Twin-Strep-tag IVDIQSGGSAWSHPQFEKGG DHVDLASAAGPAVRLELFSS t c g t g g a c a t c c a g a g c g g a g g c a g c g c c t g g a g c c a c c c t c a g t t c g a g a a a g g a g g a g a g c a c c t g t a g g t c t c g c c t c c g t c g c g g a c c t c g g t g g g a g t c a a g c t c t t t c c t c c t c

780 15 20 GSGGGSGGSA 25 30 Strep-Tag II WSHPQFEK PV Twin-Strep-tag Kozak sequence GSGGGSGGSAWSHPQFEKPV AAAASASAGSPGVWLEFLRD g c a g c g g c g g c g g a a g c g g a g g c t c c g c t t g g t c c c a c c c a c a g t t c g a a a a g c c g g t c g c g t c g c c g c c g c c t t c g c c t c c g a g g c g a a c c a g g g t g g g t g t c a a g c t t t t c g g c c a g c

840 AT 1 M 1a V 5 10 15 SKGEELFTGVVPILVE EGFP 1 M 1a V 5 10 15 SKGEELFTGVVPILVE EGFP Kozak sequence G A C C A G C T C G EGFP-N ATMVSKGEELFTGVVPILVE GGHHALALLQEGPHHGDQDL c c a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c g g t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g

900 20 25 30 35 LDGDVNGHKFSVSGEGEGDA EGFP 20 25 30 35 LDGDVNGHKFSVSGEGEGDA EGFP A C C T G C C G C T G C EGFP-N LDGDVNGHKFSVSGEGEGDA QVAVYVAVLEAHGALALAIG t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t

960 40 45 50 55 TYGKLTLKFICTTGKLPVPW EGFP 40 45 50 55 TYGKLTLKFICTTGKLPVPW EGFP TYGKLTLKFICTTGKLPVPW GVALQGQLEDAGGALQGHGP c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g

1020 60 65 70 75 PTLVTTLTYGVQCFSRYPDH EGFP 60 65 70 75 PTLVTTLTYGVQCFSRYPDH EGFP PTLVTTLTYGVQCFSRYPDH GGEHGGQGVAHLAEAAVGVV c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t

1080 80 85 90 95 MKQHDFFKSAMPEGYVQERT EGFP 80 85 90 95 MKQHDFFKSAMPEGYVQERT EGFP MKQHDFFKSAMPEGYVQERT HLLVVEELGGHGFAVDLLAG t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t

1140 100 105 110 115 IFFKDDGNYKTRAEVKFEGD EGFP 100 105 110 115 IFFKDDGNYKTRAEVKFEGD EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R IFFKDDGNYKTRAEVKFEGD DEELVVAVVLGAGLHLELAV t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t

1200 120 125 130 135 TLVNRIELKGIDFKEDGNIL EGFP 120 125 130 135 TLVNRIELKGIDFKEDGNIL EGFP TLVNRIELKGIDFKEDGNIL GQHVADLQLADVELLVAVDQ c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c

1260 140 145 150 155 GHKLEYNYNSHNVYIMADKQ EGFP 140 145 150 155 GHKLEYNYNSHNVYIMADKQ EGFP GHKLEYNYNSHNVYIMADKQ PVLQLVVVVAVVDIDHGVLL g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t

1320 160 165 170 175 KNGIKVNFKIRHNIEDGSVQ EGFP 160 165 170 175 KNGIKVNFKIRHNIEDGSVQ EGFP KNGIKVNFKIRHNIEDGSVQ LVADLHVELDAVVDLVAAHL a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g

1380 180 185 190 195 LADHYQQNTPIGDGPVLLPD EGFP 180 185 190 195 LADHYQQNTPIGDGPVLLPD EGFP LADHYQQNTPIGDGPVLLPD EGVVVLLVGGDAVAGHQQGV t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t

1440 200 205 210 215 NHYLSTQSALSKDPNEKRDH EGFP 200 205 210 215 NHYLSTQSALSKDPNEKRDH EGFP C A EGFP-C NHYLSTQSALSKDPNEKRDH VVVQAGLGGQAFVGVLLAIV a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t

BsrGI 1500 220 225 230 235 MVLLEFVTAAGITLGMDELY EGFP 220 225 230 235 MVLLEFVTAAGITLGMDELY EGFP T G G T C C T G C T G G A G T T C G T G EGFP-C MVLLEFVTAAGITLGMDELY HDQQLEHGGGPDSEAHVLQV t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t

1560 K (in frame with EGFP) SGRTQIRQHLEFSHNEGTL EGFP K (in frame with EGFP) SGRTQIRQHLEFSHNEGTL EGFP KSGRTQIRQHLEFSHNEGTL LGAPSLDPLVQLEAVVLAGQ a g t c c g g c c g g a c t c a g a t c c g g c a g c a c c t g g a g t t c a g c c a c a a c g a g g g c a c c c t g a t c a g g c c g g c c t g a g t c t a g g c c g t c g t g g a c c t c a a g t c g g t g t t g c t c c c g t g g g a c t

BstEII 1620 (in frame with EGFP) SSGFLQEKVWVMLVTSLVAL (in frame with EGFP) SSGFLQEKVWVMLVTSLVAL SSGFLQEKVWVMLVTSLVAL AAAEELLLHPHHQHGAQHSE g c a g c g g c t t c c t c c a g g a g a a g g t g t g g g t g a t g c t g g t g a c c a g c c t g g t g g c t c t c c c g t c g c c g a a g g a g g t c c t c t t c c a c a c c c a c t a c g a c c a c t g g t c g g a c c a c c g a g a g g

SacIIPspOMITspMIXmaIApaIPstISalIAccIEcoRIBglIIPaeR7IXhoIEco53kISacIHindIII 1680 QAL* MCS QAL* QAL* LGQLSRSSLSRIRCDVTGRA a g g c c c t g t a g a g a t c t c g a g c t c a a g c t t c g a a t t c t g c a g t c g a c g g t a c c g c g g g c c t c c g g g a c a t c t c t a g a g c t c g a g t t c g a a g c t t a a g a c g t c a g c t g c c a t g g c g c c c g g

BclI*XbaI*SmaIBamHI 1740 MCS RSGGSRSLQDYDAM c g g g a t c c a c c g g a t c t a g a t a a c t g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t g c c c t a g g t g g c c t a g a t c t a t t g a c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a

MfeI 1800 t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t

HpaI 1860 SV40 poly(A) signal C G T T A T C G T A G T SV40pA-R t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t

1920 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A EBV-rev G T T T A A A G SV40pA-R c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t

MluI 1980 SV40 poly(A) signal f1 ori T C EBV-rev t c a a t g t a t c t t a a c g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t a g t t a c a t a g a a t t g c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a

2040 f1 ori t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t

2100 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t

2160 f1 ori t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t

DraIII 2220 f1 ori c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a

2280 f1 ori c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c

2340 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g

2400 f1 ori a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a

2460 AmpR promoter g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t

2520 AmpR promoter a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c

2580 AmpR promoter SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a

SexAI*CsiI 2640 SV40 promoter SV40 enhancer c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t

2700 SV40 promoter SV40 enhancer g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a

2760 SV40 promoter SV40 enhancer SV40 ori a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a

2820 SV40 promoter SV40 ori c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c

StuISfiI 2880 SV40 promoter SV40 ori c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a

BspDI*ClaI* 2940 1 5 MIEQDGL SV40 promoter NeoR/KanR 1 5 MIEQDGL NeoR/KanR MIEQDGL g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c

3000 10 15 20 25 HAGSPAAWVERLFGYDWAQQ NeoR/KanR 10 15 20 25 HAGSPAAWVERLFGYDWAQQ NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R HAGSPAAWVERLFGYDWAQQ c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c

KasINarISfoIPluTI 3060 30 35 40 45 TIGCSDAAVFRLSAQGRPVL NeoR/KanR 30 35 40 45 TIGCSDAAVFRLSAQGRPVL NeoR/KanR TIGCSDAAVFRLSAQGRPVL a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a

3120 50 55 60 65 FVKTDLSGALNELQDEAARL NeoR/KanR 50 55 60 65 FVKTDLSGALNELQDEAARL NeoR/KanR FVKTDLSGALNELQDEAARL t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t

Tth111IPflFIFspI 3180 70 75 80 85 SWLATTGVPCAAVLDVVTEA NeoR/KanR 70 75 80 85 SWLATTGVPCAAVLDVVTEA NeoR/KanR SWLATTGVPCAAVLDVVTEA t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c

3240 90 95 100 105 GRDWLLLGEVPGQDLLSSHL NeoR/KanR 90 95 100 105 GRDWLLLGEVPGQDLLSSHL NeoR/KanR GRDWLLLGEVPGQDLLSSHL *RVK g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a

BsrDI 3300 110 115 120 125 APAEKVSIMADAMRRLHTLD NeoR/KanR 110 115 120 125 APAEKVSIMADAMRRLHTLD NeoR/KanR APAEKVSIMADAMRRLHTLD SRGLFYGDHSICHPPQMRKI g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a

3360 130 135 140 145 PATCPFDHQAKHRIERARTR NeoR/KanR 130 135 140 145 PATCPFDHQAKHRIERARTR NeoR/KanR PATCPFDHQAKHRIERARTR RSGAWEVVLRFMADLSCTSP c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c

3420 150 155 160 165 MEAGLVDQDDLDEEHQGLAP NeoR/KanR 150 155 160 165 MEAGLVDQDDLDEEHQGLAP NeoR/KanR MEAGLVDQDDLDEEHQGLAP HFGTKDILIIQVFLMLPERW a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t

3480 170 175 180 185 AELFARLKASMPDGEDLVVT NeoR/KanR 170 175 180 185 AELFARLKASMPDGEDLVVT NeoR/KanR AELFARLKASMPDGEDLVVT GFQEGPELRAHGVALIEDHG g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g

3540 190 195 200 205 HGDACLPNIMVENGRFSGFI NeoR/KanR 190 195 200 205 HGDACLPNIMVENGRFSGFI NeoR/KanR HGDACLPNIMVENGRFSGFI MAIGAQRIDHHFIAAKRSED c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g

RsrII 3600 210 215 220 225 DCGRLGVADRYQDIALATRD NeoR/KanR 210 215 220 225 DCGRLGVADRYQDIALATRD NeoR/KanR C G T T G G C T A C C C G T G A T Neo-F DCGRLGVADRYQDIALATRD VTAPQTHRVAILVYRQSGTI g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a

3660 230 235 240 245 IAEELGGEWADRFLVLYGIA NeoR/KanR 230 235 240 245 IAEELGGEWADRFLVLYGIA NeoR/KanR A T T Neo-F IAEELGGEWADRFLVLYGIA NSFLKAAFPSVAEEHKVTDG a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g

3720 250 255 260 265 APDSQRIAFYRLLDEFF* NeoR/KanR 250 255 260 265 APDSQRIAFYRLLDEFF* NeoR/KanR APDSQRIAFYRLLDEFF* SGIRLADGEIAKKVLEESRS g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t

3780 MTDQATPNLPSRDFD EPTRFSRGLSAWGAM c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a

3840 STAAFYERLGFGIVFRDAGW c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t

3900 MILQRGDLMLEFFAHPRGRL t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t

3960 HSV TK poly(A) signal A C T T T G T G C C T T C C T C T G T T TK-pA-R TETRKETIPEGTRAMTAIKR c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g

4020 HSV TK poly(A) signal QNKTHGVGSFVHKRGVRSQG a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a

BsaI 4080 WHSVDTPPRPHWGQYARVSS g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a

4140 FSPPHPPSSGEGPGLAANVG t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c

4200 AAGPAIASGYSYIL* c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g

4260 a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g

4320 c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a

4380 ori c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g

4440 ori c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a

4500 ori t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a

4560 ori t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c

4620 ori c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t

ApaLI 4680 ori a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t

4740 ori c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c

4800 ori g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c

4860 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g

4920 ori t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t

PciI 4980 a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c

3ʹ 5ʹ 5024 c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a t g c a t g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.