Addgene pGag-mCherry Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pGag-mCherry Sequencing Result


Map View

SfiI (6974) AvrII (6920) BstAPI (6827) NaeI (6590) NgoMIV (6588) pBR322ori-F (6336 .. 6355) ScaI (5246) Amp-R (5158 .. 5177) XmnI (5127) pBRforEco (4749 .. 4767) XbaI (4725) AccI (4621) BspQI - SapI (4230) SwaI (4166) BsgI (3978) HpaI (3922) SphI (3872) BGH-rev (3666 .. 3683) BclI * (3659) SpeI (96) CMV enhancer NdeI (331) SnaBI (437) BtgI - NcoI (457) CMV-F (616 .. 636) LNCX (662 .. 686) AflIII - PciI (762) Acc65I (768) KpnI (772) BamHI (780) CsiI - SexAI * (1561) PflFI - Tth111I (1563) Bpu10I (1636) pLXSN 5' (1678 .. 1700) pBABE 5' (1716 .. 1732) PvuII (2144) PaeR7I - PspXI - XhoI (2268) PspOMI (2406) ApaI (2410) BglII (2614) NruI * (2830) EcoRV (3185) PfoI (3187) BstBI (3247) SbfI (3292) pGag-mCherry 7017 bp

Sequence View

60 a g c t t g g c c c a t t g c a t a c g t t g t a t c c a t a t c a t a a t a t g t a c a t t t a t a t t g g c t c a t t c g a a c c g g g t a a c g t a t g c a a c a t a g g t a t a g t a t t a t a c a t g t a a a t a t a a c c g a g t a

SpeI 120 CMV enhancer g t c c a a c a t t a c c g c c a t g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c a g g t t g t a a t g g c g g t a c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t

180 CMV enhancer c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c

240 CMV enhancer g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g

300 CMV enhancer c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t

NdeI 360 CMV enhancer c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t

420 CMV enhancer a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t

NcoIBtgISnaBI 480 CMV enhancer CMV promoter c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a

540 CMV promoter a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c

600 CMV promoter a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t

660 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t

720 CMV promoter A G C T C G T T T A G T G A A C C G T C A G A T C LNCX g a g c t c g t t t a g t g a a c c g t c a g a t c g c c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c c t c g a g c a a a t c a c t t g g c a g t c t a g c g g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g

AflIIIPciIAcc65IKpnI 780 a t a g a a g a c a c c g g g a c c g a t c c a g c c t c c c c t c g a a g c t t a c a t g t g g t a c c g a g c t c g t a t c t t c t g t g g c c c t g g c t a g g t c g g a g g g g a g c t t c g a a t g t a c a c c a t g g c t c g a g c

BamHI 840 β- globin intron g a t c c t g a g a a c t t c a g g g t g a g t c t a t g g g a c c c t t g a t g t t t t c t t t c c c c t t c t t t t c t a g g a c t c t t g a a g t c c c a c t c a g a t a c c c t g g g a a c t a c a a a a g a a a g g g g a a g a a a a

900 β- globin intron c t a t g g t t a a g t t c a t g t c a t a g g a a g g g g a g a a g t a a c a g g g t a c a c a t a t t g a c c a a a g a t a c c a a t t c a a g t a c a g t a t c c t t c c c c t c t t c a t t g t c c c a t g t g t a t a a c t g g t t t

960 β- globin intron t c a g g g t a a t t t t g c a t t t g t a a t t t t a a a a a a t g c t t t c t t c t t t t a a t a t a c t t t t t t a g t c c c a t t a a a a c g t a a a c a t t a a a a t t t t t t a c g a a a g a a g a a a a t t a t a t g a a a a a a

1020 β- globin intron g t t t a t c t t a t t t c t a a t a c t t t c c c t a a t c t c t t t c t t t c a g g g c a a t a a t g a t a c a a t c a a a t a g a a t a a a g a t t a t g a a a g g g a t t a g a g a a a g a a a g t c c c g t t a t t a c t a t g t t a

1080 β- globin intron g t a t c a t g c c t c t t t g c a c c a t t c t a a a g a a t a a c a g t g a t a a t t t c t g g g t t a a g g c a a c a t a g t a c g g a g a a a c g t g g t a a g a t t t c t t a t t g t c a c t a t t a a a g a c c c a a t t c c g t t

1140 β- globin intron t a g c a a t a t t t c t g c a t a t a a a t a t t t c t g c a t a t a a a t t g t a a c t g a t g t a a g a g g t t t a t c g t t a t a a a g a c g t a t a t t t a t a a a g a c g t a t a t t t a a c a t t g a c t a c a t t c t c c a a a

1200 β- globin intron c a t a t t g c t a a t a g c a g c t a c a a t c c a g c t a c c a t t c t g c t t t t a t t t t a t g g t t g g g a t g t a t a a c g a t t a t c g t c g a t g t t a g g t c g a t g g t a a g a c g a a a a t a a a a t a c c a a c c c t a

1260 β- globin intron MFIPL a a g g c t g g a t t a t t c t g a g t c c a a g c t a g g c c c t t t t g c t a a t c a t g t t c a t a c c t c t t a t t c c g a c c t a a t a a g a c t c a g g t t c g a t c c g g g a a a a c g a t t a g t a c a a g t a t g g a g a a t

1320 β- globin intron IFLPQLLGNVLVCVLAHHFG t c t t c c t c c c a c a g c t c c t g g g c a a c g t g c t g g t c t g t g t g c t g g c c c a t c a c t t t g g c a a g a a g g a g g g t g t c g a g g a c c c g t t g c a c g a c c a g a c a c a c g a c c g g g t a g t g a a a c c g t

1380 gag (truncated) KEFMGQTVTTPLSLTLGHWK a a g a a t t c a t g g g c c a g a c t g t t a c c a c t c c c t t a a g t t t g a c c t t a g g t c a c t g g a a a g t t c t t a a g t a c c c g g t c t g a c a a t g g t g a g g g a a t t c a a a c t g g a a t c c a g t g a c c t t t c

1440 gag (truncated) DVERIAHNQSVDVKKRRWVT a t g t c g a g c g g a t c g c t c a c a a c c a g t c g g t a g a t g t c a a g a a g a g a c g t t g g g t t a c c t t a c a g c t c g c c t a g c g a g t g t t g g t c a g c c a t c t a c a g t t c t t c t c t g c a a c c c a a t g g a

1500 gag (truncated) FCSAEWPTFNVGWPRDGTFN t c t g c t c t g c a g a a t g g c c a a c c t t t a a c g t c g g a t g g c c g c g a g a c g g c a c c t t t a a c c a g a c g a g a c g t c t t a c c g g t t g g a a a t t g c a g c c t a c c g g c g c t c t g c c g t g g a a a t t g g

1560 gag (truncated) RDLITQVKIKVFSPGPHGHP g a g a c c t c a t c a c c c a g g t t a a g a t c a a g g t c t t t t c a c c t g g c c c g c a t g g a c a c c c a g c t c t g g a g t a g t g g g t c c a a t t c t a g t t c c a g a a a a g t g g a c c g g g c g t a c c t g t g g g t c

SexAI*CsiITth111IPflFI 1620 gag (truncated) DQVPYIVTWEALAFDPPPWV a c c a g g t c c c c t a c a t c g t g a c c t g g g a a g c c t t g g c t t t t g a c c c c c c t c c c t g g g t c a t g g t c c a g g g g a t g t a g c a c t g g a c c c t t c g g a a c c g a a a a c t g g g g g g a g g g a c c c a g t

Bpu10I 1680 gag (truncated) C C C pLXSN 5' KPFVHPKPPPPLPPSAPSLP a g c c c t t t g t a c a c c c t a a g c c t c c g c c t c c t c t t c c t c c a t c c g c c c c g t c t c t c c c c c t c g g g a a a c a t g t g g g a t t c g g a g g c g g a g g a g a a g g a g g t a g g c g g g g c a g a g a g g g g g

1740 gag (truncated) T T G A A C C T C C T C G T T C G A C C pLXSN 5' C T T T A T C C A G C C C T C A C pBABE 5' LEPPRSTPPRSSLYPALTPS t t g a a c c t c c t c g t t c g a c c c c g c c t c g a t c c t c c c t t t a t c c a g c c c t c a c t c c t t c t c a a c t t g g a g g a g c a a g c t g g g g c g g a g c t a g g a g g g a a a t a g g t c g g g a g t g a g g a a g a g

1800 gag (truncated) LGAKPKPQVLSDSGGPLIDL t a g g c g c c a a a c c t a a a c c t c a a g t t c t t t c t g a c a g t g g g g g g c c g c t c a t c g a c c t a c a t c c g c g g t t t g g a t t t g g a g t t c a a g a a a g a c t g t c a c c c c c c g g c g a g t a g c t g g a t g

1860 LTEDPPPYRDPRPPPSDRDG t t a c a g a a g a c c c c c c g c c t t a t a g g g a c c c a a g a c c a c c c c c t t c c g a c a g g g a c g g a a a a t g t c t t c t g g g g g g c g g a a t a t c c c t g g g t t c t g g t g g g g g a a g g c t g t c c c t g c c t t

1920 NGGEATPAGEAPDPSPMASR a t g g t g g a g a a g c g a c c c c t g c g g g a g a g g c a c c g g a c c c c t c c c c a a t g g c a t c t c g c c t a c c a c c t c t t c g c t g g g g a c g c c c t c t c c g t g g c c t g g g g a g g g g t t a c c g t a g a g c g g

1980 LRGRREPPVADSTTSQAFPL t a c g t g g g a g a c g g g a g c c c c c t g t g g c c g a c t c c a c t a c c t c g c a g g c a t t c c c c c t c c a t g c a c c c t c t g c c c t c g g g g g a c a c c g g c t g a g g t g a t g g a g c g t c c g t a a g g g g g a g g

2040 RAGGNGQLQYWPFSSSDLYN g c g c a g g a g g a a a c g g a c a g c t t c a a t a c t g g c c g t t c t c c t c t t c t g a c c t t t a c a a c t c g c g t c c t c c t t t g c c t g t c g a a g t t a t g a c c g g c a a g a g g a g a a g a c t g g a a a t g t t g a

2100 WKNNNPSFSEDPGKLTALIE g g a a a a a t a a t a a c c c t t c t t t t t c t g a a g a t c c a g g t a a a c t g a c a g c t c t g a t c g a g t c c t t t t t a t t a t t g g g a a g a a a a a g a c t t c t a g g t c c a t t t g a c t g t c g a g a c t a g c t c a

PvuII 2160 SVLITHQPTWDDCQQLLGTL c t g t c c t c a t c a c c c a t c a g c c c a c c t g g g a c g a c t g t c a g c a g c t g t t g g g g a c t c t g c g a c a g g a g t a g t g g g t a g t c g g g t g g a c c c t g c t g a c a g t c g t c g a c a a c c c c t g a g a c g

2220 LTGEEKQRVLLEARKAVRGD t g a c c g g a g a a g a a a a a c a a c g g g t g c t c t t a g a g g c t a g a a a g g c g g t g c g g g g c g a t g a c t g g c c t c t t c t t t t t g t t g c c c a c g a g a a t c t c c g a t c t t t c c g c c a c g c c c c g c t a c

PaeR7IPspXIXhoI 2280 DGRPTQLPNEVDAAFPLERP a t g g g c g c c c c a c t c a a c t g c c c a a t g a a g t c g a t g c c g c t t t t c c c c t c g a g c g c c c a g t a c c c g c g g g g t g a g t t g a c g g g t t a c t t c a g c t a c g g c g a a a a g g g g a g c t c g c g g g t c

2340 DWDYTTQAGRNHLVHYRQLL a c t g g g a t t a c a c c a c c c a g g c a g g t a g g a a c c a c c t a g t c c a c t a t c g c c a g t t g c t c c t g a c c c t a a t g t g g t g g g t c c g t c c a t c c t t g g t g g a t c a g g t g a t a g c g g t c a a c g a g g

2400 LAGLQNAGRSPTNLAKVKGI t a g c g g g t c t c c a a a a c g c g g g c a g a a g c c c c a c c a a t t t g g c c a a g g t a a a a g g a a t a a a t c g c c c a g a g g t t t t g c g c c c g t c t t c g g g g t g g t t a a a c c g g t t c c a t t t t c c t t a t t

PspOMIApaI 2460 TQGPNESPSAFLERLKEAYR c a c a a g g g c c c a a t g a g t c t c c c t c g g c c t t c c t a g a g a g a c t t a a g g a a g c c t a t c g c a g t g t t c c c g g g t t a c t c a g a g g g a g c c g g a a g g a t c t c t c t g a a t t c c t t c g g a t a g c g t

2520 RYTPYDPEDPGQETNVSMSF g g t a c a c t c c t t a t g a c c c t g a g g a c c c a g g g c a a g a a a c t a a t g t g t c t a t g t c t t t c a c c a t g t g a g g a a t a c t g g g a c t c c t g g g t c c c g t t c t t t g a t t a c a c a g a t a c a g a a a g t

2580 IWQSAPDIGRKLERLEDLKN t t t g g c a g t c t g c c c c a g a c a t t g g g a g a a a g t t a g a g a g g t t a g a a g a t t t a a a a a a c a a a a c c g t c a g a c g g g g t c t g t a a c c c t c t t t c a a t c t c t c c a a t c t t c t a a a t t t t t t g t

BglII 2640 KTLGDLVREAEKIFNKRETP a g a c g c t t g g a g a t t t g g t t a g a g a g g c a g a a a a g a t c t t t a a t a a a c g a g a a a c c c c g g t c t g c g a a c c t c t a a a c c a a t c t c t c c g t c t t t t c t a g a a a t t a t t t g c t c t t t g g g g c c

2700 EEREERIRRETEEKEERRRT a a g a a a g a g a g g a a c g t a t c a g g a g a g a a a c a g a g g a a a a a g a a g a a c g c c g t a g g a c a g t t c t t t c t c t c c t t g c a t a g t c c t c t c t t t g t c t c c t t t t t c t t c t t g c g g c a t c c t g t c

2760 EDEQKEKERDRRRHREMSKL a g g a t g a g c a g a a a g a g a a a g a a a g a g a t c g t a g g a g a c a t a g a g a g a t g a g c a a g c t a t t c c t a c t c g t c t t t c t c t t t c t t t c t c t a g c a t c c t c t g t a t c t c t c t a c t c g t t c g a t a

2820 LATVVSGQKQDRQGGERRRS t g g c c a c t g t c g t t a g t g g a c a g a a a c a g g a t a g a c a g g g a g g a g a a c g a a g g a g g t c c c a c c g g t g a c a g c a a t c a c c t g t c t t t g t c c t a t c t g t c c c t c c t c t t g c t t c c t c c a g g g

NruI* 2880 QLDRDQCAYCKEKGHWAKDC a a c t c g a t c g c g a c c a g t g t g c c t a c t g c a a a g a a a a g g g g c a c t g g g c t a a a g a t t g t c t t g a g c t a g c g c t g g t c a c a c g g a t g a c g t t t c t t t t c c c c g t g a c c c g a t t t c t a a c a g

2940 PKKPRGPRGPRPQGSGSGSG c c a a g a a a c c a c g a g g a c c t c g g g g a c c a a g a c c g c a g g g a t c a g g a t c a g g a t c a g g a g g g t t c t t t g g t g c t c c t g g a g c c c c t g g t t c t g g c g t c c c t a g t c c t a g t c c t a g t c c t c

3000 VSKGEEDNMAIIKEFMRFKV t g t c c a a g g g c g a g g a a g a t a a c a t g g c c a t c a t c a a g g a g t t c a t g a g g t t t a a g g t c c a c a g g t t c c c g c t c c t t c t a t t g t a c c g g t a g t a g t t c c t c a a g t a c t c c a a a t t c c a g g

3060 HMEGSVNGHEFEIEGEGEGR a c a t g g a g g g t t c a g t c a a t g g c c a c g a g t t c g a g a t t g a a g g c g a g g g c g a g g g c c g c c t g t a c c t c c c a a g t c a g t t a c c g g t g c t c a a g c t c t a a c t t c c g c t c c c g c t c c c g g c g g

3120 PYEGTQTAKLKVTKGGPLPF c c t a c g a a g g g a c a c a g a c g g c g a a a t t g a a g g t g a c c a a a g g c g g g c c a t t g c c c t t c g g g a t g c t t c c c t g t g t c t g c c g c t t t a a c t t c c a c t g g t t t c c g c c c g g t a a c g g g a a g c

3180 AWDILSPQFMYGSKAYVKHP c a t g g g a c a t c t t g t c c c c t c a g t t t a t g t a t g g c a g c a a g g c c t a t g t t a a g c a c c c c g g t a c c c t g t a g a a c a g g g g a g t c a a a t a c a t a c c g t c g t t c c g g a t a c a a t t c g t g g g g c

EcoRVPfoI 3240 ADIPDYLKLSFPEGFKWERV c t g a t a t c c c g g a c t a c t t g a a g c t g t c c t t t c c a g a g g g g t t t a a a t g g g a g c g c g t t a g a c t a t a g g g c c t g a t g a a c t t c g a c a g g a a a g g t c t c c c c a a a t t t a c c c t c g c g c a a t

SbfIBstBI 3300 MNFEDGGVVTVTQDSSLQDG t g a a t t t c g a a g a c g g a g g a g t g g t t a c g g t g a c g c a g g a c t c a t c c c t g c a g g a c g g a g a c t t a a a g c t t c t g c c t c c t c a c c a a t g c c a c t g c g t c c t g a g t a g g g a c g t c c t g c c t c

3360 EFIYKVKLRGTNFPSDGPVM a a t t t a t a t a t a a g g t t a a g t t g a g a g g c a c a a a c t t c c c a a g c g a c g g c c c t g t g a t g c t t a a a t a t a t a t t c c a a t t c a a c t c t c c g t g t t t g a a g g g t t c g c t g c c g g g a c a c t a c g

3420 QKKTMGWEASSERMYPEDGA a g a a g a a a a c a a t g g g g t g g g a a g c t t c c a g c g a g c g c a t g t a c c c c g a a g a t g g c g c c c t c t t c t t t t g t t a c c c c a c c c t t c g a a g g t c g c t c g c g t a c a t g g g g c t t c t a c c g c g g g

3480 LKGEIKQRLKLKDGGHYDAE t c a a g g g c g a g a t a a a g c a a a g g c t g a a a c t t a a g g a c g g c g g t c a t t a c g a c g c g g a g g a g t t c c c g c t c t a t t t c g t t t c c g a c t t t g a a t t c c t g c c g c c a g t a a t g c t g c g c c t c c

3540 VKTTYKAKKPVQLPGAYNVN t c a a g a c a a c t t a c a a g g c t a a a a a a c c c g t t c a g t t g c c t g g g g c t t a c a a t g t t a a t a a g t t c t g t t g a a t g t t c c g a t t t t t t g g g c a a g t c a a c g g a c c c c g a a t g t t a c a a t t a t

3600 IKLDITSHNEDYTIVEQYER t c a a a c t t g a c a t c a c a a g c c a c a a t g a a g a c t a t a c g a t c g t g g a g c a g t a t g a a c g a g a g t t t g a a c t g t a g t g t t c g g t g t t a c t t c t g a t a t g c t a g c a c c t c g t c a t a c t t g c t c

BclI* 3660 AEGRHSTGGMDELYK* c g g a a g g c a g g c a c t c a a c g g g g g g g a t g g a c g a g c t t t a c a a g t a a c t a g a g c t c g c t g g c c t t c c g t c c g t g a g t t g c c c c c c c t a c c t g c t c g a a a t g t t c a t t g a t c t c g a g c g a c

3720 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev a t c a g c c t c g a c t g t g c c t t c t a g t t a c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t a g t c g g a g c t g a c a c g g a a g a t c a a t g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g

3780 bGH poly(A) signal t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g

3840 bGH poly(A) signal a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t

SphI 3900 bGH poly(A) signal g g g g g a g g a t t g g g a a g a g a a t a g c a g g c a t g c t g g g g a c a t g g c g c g t t c a a c g c t c t c c c c c c t c c t a a c c c t t c t c t t a t c g t c c g t a c g a c c c c t g t a c c g c g c a a g t t g c g a g a g

HpaI 3960 a a a a c c c c t t a a a a a t a a g g t t a a c c c g c g a g g c c c c c t a a g a a t t c a c c c c a c c a g t g c t t t t g g g g a a t t t t t a t t c c a a t t g g g c g c t c c g g g g g a t t c t t a a g t g g g g t g g t c a c g

BsgI 4020 a g g c t g c c t a t c a g a a a g t g g t g g c t g g t g t g g c t a a t g c c c t g g c c c a c a a g t a t c a c t t c c g a c g g a t a g t c t t t c a c c a c c g a c c a c a c c g a t t a c g g g a c c g g g t g t t c a t a g t g a

4080 β- globin poly(A) a a g c t c g c t t t c t t g c t g t c c a a t t t c t a t t a a a g g t t c c t t t g t t c c c t a a g t c c a a c t t t c g a g c g a a a g a a c g a c a g g t t a a a g a t a a t t t c c a a g g a a a c a a g g g a t t c a g g t t g a

4140 β- globin poly(A) a c t a a a c t g g g g g a t a t t a t g a a g g g c c t t g a g c a t c t g g a t t c t g c c t a a t a a a a a a c a t g a t t t g a c c c c c t a t a a t a c t t c c c g g a a c t c g t a g a c c t a a g a c g g a t t a t t t t t t g t

SwaI 4200 β- globin poly(A) t t t a t t t t c a t t g c a a t g a t g t a t t t a a a t t a t t t c t g a a t a t t t t a c t a a a a a g g g a a t a a a t a a a a g t a a c g t t a c t a c a t a a a t t t a a t a a a g a c t t a t a a a a t g a t t t t t c c c t t a

BspQISapI 4260 β- globin poly(A) g t g g g a g g t c a g t g c a t t t a a a a c a t a a a g a a a t g a a g a g c t a g t t c a a a c c t t g g g a a a c a c c c t c c a g t c a c g t a a a t t t t g t a t t t c t t t a c t t c t c g a t c a a g t t t g g a a c c c t t t

4320 β- globin poly(A) a t a c a c t a t a t c t t a a a c t c c a t g a a a g a a g g t g a g g c t g c a a a c a g c t a a t g c a c a t t g t a t g t g a t a t a g a a t t t g a g g t a c t t t c t t c c a c t c c g a c g t t t g t c g a t t a c g t g t a a c

4380 β- globin poly(A) g c a a c a g c c c c t g a t g c c t a t g c c t t a t t c a t c c c t c a g a a a a g g a t t c a a g t a g a g g c t c g t t g t c g g g g a c t a c g g a t a c g g a a t a a g t a g g g a g t c t t t t c c t a a g t t c a t c t c c g a

4440 β- globin poly(A) t g a t t t g g a g g t t a a a g t t t t g c t a t g c t g t a t t t t a c a t t a c t t a t t g t t t t a g c t g t c a c t a a a c c t c c a a t t t c a a a a c g a t a c g a c a t a a a a t g t a a t g a a t a a c a a a a t c g a c a g

4500 c t c a t g a a t g t c t t t t c a c t a c c c a t t t g c t t a t c c t g c a t c t c t c a g c c t t g a c t c c a c g a g t a c t t a c a g a a a a g t g a t g g g t a a a c g a a t a g g a c g t a g a g a g t c g g a a c t g a g g t g

4560 t c a g t t c t c t t g c t t a g a g a t a c c a c c t t t c c c c t g a a g t g t t c c t t c c a t g t t t t a c g g a g t c a a g a g a a c g a a t c t c t a t g g t g g a a a g g g g a c t t c a c a a g g a a g g t a c a a a a t g c c

4620 c g a g a t g g t t t c t c c t c g c c t g g c c a c t c a g c c t t a g t t g t c t c t g t t g t c t t a t a g a g g g c t c t a c c a a a g a g g a g c g g a c c g g t g a g t c g g a a t c a a c a g a g a c a a c a g a a t a t c t c c

AccI 4680 t c t a c t t g a a g a a g g a a a a a c a g g g g g c a t g g t t t g a c t g t c c t g t g a g c c c t t c t t c c c a g a t g a a c t t c t t c c t t t t t g t c c c c c g t a c c a a a c t g a c a g g a c a c t c g g g a a g a a g g g

XbaI 4740 t g c c t c c c c c a c t c a c a g t g a c c c g g a a t c c c t c g a c a t g g c a g t c t a g a a t t c t t g a a g a c g g a g g g g g t g a g t g t c a c t g g g c c t t a g g g a g c t g t a c c g t c a g a t c t t a a g a a c t t c

4800 C G G A G C A C T A T G C G G A T A A pBRforEco a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g

4860 AmpR promoter t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a

4920 AmpR promoter c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t

4980 1 5 10 signal sequence MSIQHFRVALIPFF AmpR promoter AmpR MSIQHFRVALIPFF a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a

5040 15 20 signal sequence AAFCLPVFA 25 30 HPETLVKVKDA AmpR AAFCLPVFAHPETLVKVKDA t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g

5100 35 40 45 50 EDQLGARVGYIELDLNSGKI AmpR EDQLGARVGYIELDLNSGKI t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a

XmnI 5160 55 60 65 70 LESFRPEERFPMMSTFKVLL AmpR C G A Amp-R LESFRPEERFPMMSTFKVLL c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a

5220 75 80 85 90 CGAVLSRVDAGQEQLGRRIH AmpR T A C A C C G C G C C A T A A T A Amp-R CGAVLSRVDAGQEQLGRRIH a t g t g g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a t a c a c c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t

ScaI 5280 95 100 105 110 YSQNDLVEYSPVTEKHLTDG AmpR YSQNDLVEYSPVTEKHLTDG c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c

5340 115 120 125 130 MTVRELCSAAITMSDNTAAN AmpR MTVRELCSAAITMSDNTAAN c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t

5400 135 140 145 150 LLLTTIGGPKELTAFLHNMG AmpR LLLTTIGGPKELTAFLHNMG c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c

5460 155 160 165 170 DHVTRLDRWEPELNEAIPND AmpR DHVTRLDRWEPELNEAIPND g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t

5520 175 180 185 190 ERDTTMPAAMATTLRKLLTG AmpR ERDTTMPAAMATTLRKLLTG c g a g c g t g a c a c c a c g a t g c c t g c a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g g c t c g c a c t g t g g t g c t a c g g a c g t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c

5580 195 200 205 210 ELLTLASRQQLIDWMEADKV AmpR ELLTLASRQQLIDWMEADKV c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a

5640 215 220 225 230 AGPLLRSALPAGWFIADKSG AmpR AGPLLRSALPAGWFIADKSG t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c

5700 235 240 245 250 AGERGSRGIIAALGPDGKPS AmpR AGERGSRGIIAALGPDGKPS a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g

5760 255 260 265 270 RIVVIYTTGSQATMDERNRQ AmpR RIVVIYTTGSQATMDERNRQ c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t

5820 275 280 285 IAEIGASLIKHW* AmpR IAEIGASLIKHW* g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g

5880 a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g c t g c t g c t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c g a c g a c g

5940 t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t

6000 a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t

6060 g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a

6120 c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c

6180 g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g

6240 a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t

6300 t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c

6360 G G G A A A C G C C T G G T A T C T T T pBR322ori-F g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a

6420 c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c

6480 c g g a g c c t a t g g a a a a a c g c c a g c a a c g g a g a t g c g c c g c g t g c g g c t g c t g g a g a t g g c g c c t c g g a t a c c t t t t t g c g g t c g t t g c c t c t a c g c g g c g c a c g c c g a c g a c c t c t a c c g

6540 g g a c g c g a t g g a t a t g t t c t g c c a a g g g t t g g t t t g c g c a t t c a c a g t t c t c c g c a a g a a c c t g c g c t a c c t a t a c a a g a c g g t t c c c a a c c a a a c g c g t a a g t g t c a a g a g g c g t t c t t

NgoMIVNaeI 6600 t t g a t t g g c t c c a a t t c t t g g a g t g g t g a a t c c g t t a g c g a g g t g c c g c c g g c t t c c a t t a a c t a a c c g a g g t t a a g a a c c t c a c c a c t t a g g c a a t c g c t c c a c g g c g g c c g a a g g t a a

6660 c a g g t c g a g g t g g c c c g g c t c c a t g c a c c g c g a c g c a a c g c g g g g a g g c a g a c a a g g t a t g t c c a g c t c c a c c g g g c c g a g g t a c g t g g c g c t g c g t t g c g c c c c t c c g t c t g t t c c a t a

6720 a g g g c g g c g c c t a c a a t c c a t g c c a a c c c g t t c c a t g t g c t c g c c g a g g c g g c a t a a a t c t c c c g c c g c g g a t g t t a g g t a c g g t t g g g c a a g g t a c a c g a g c g g c t c c g c c g t a t t t a g

6780 g c c g t g a c g a t c a g c g g t c c a a t g a t c g a a g t t a g g c t g g t a a g a g c c g c g a g c g a t c c t c g g c a c t g c t a g t c g c c a g g t t a c t a g c t t c a a t c c g a c c a t t c t c g g c g c t c g c t a g g a

BstAPI 6840 t g a a g c t g t c c c t g a t g g t c g t c a t c t a c c t g c c t g g a c a g c a t g g c c t g c a a c g c g g g c a c t t c g a c a g g g a c t a c c a g c a g t a g a t g g a c g g a c c t g t c g t a c c g g a c g t t g c g c c c g

6900 a t c c c g a t g c c g c c g g a a g c g a g a a g a a t c a t a a t g g g g a a g g c c a t c c a g c c t c g c g t c t a g g g c t a c g g c g g c c t t c g c t c t t c t t a g t a t t a c c c c t t c c g g t a g g t c g g a g c g c a g

AvrII 6960 g g g g a g c t t t t t g c a a a a g c c t a g g c c t c c a a a a a a g c c t c c t c a c t a c t t c t g g a a t a g c c c c t c g a a a a a c g t t t t c g g a t c c g g a g g t t t t t t c g g a g g a g t g a t g a a g a c c t t a t c

SfiI 7017 c t c a g a g g c c g a g g c g g c c t c g g c c t c t g c a t a a a t a a a a a a a a t t a g t c a g c c a t g g a g t c t c c g g c t c c g c c g g a g c c g g a g a c g t a t t t a t t t t t t t t a a t c a g t c g g t a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.