Addgene pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS Sequencing Result


Map View

M13 rev M13 Reverse (9946 .. 9962) lac operator M13/pUC Reverse (9927 .. 9949) CAP binding site SfiI (9725) L4440 (9591 .. 9608) pBR322ori-F (9338 .. 9357) FspI (8382) PvuI (8236) Amp-R (8036 .. 8055) SspI (7800) pBRforEco (7627 .. 7645) PacI (7590) SmaI (7582) TspMI - XmaI (7580) PsiI (7543) SacII (7115) WPRE-R (6665 .. 6685) BspDI - ClaI (6600) BsrGI (6453) SalI (6140) PflFI - Tth111I (6137) BstXI (5902) IRES-F (5710 .. 5729) PaqCI (5655) IRES reverse (5483 .. 5500) ApaI (5433) PspOMI (5429) PaeR7I - PspXI - XhoI (5306) EBV-rev (10,012 .. 10,031) BspEI (308) NruI * (833) FseI (1150) MfeI (1189) BbvCI (1424) AleI (1577) KflI - PpuMI (1934) SpeI (2212) SnaBI (2506) CMV promoter CMV-F (2685 .. 2705) LNCX (2731 .. 2755) BsmBI - Esp3I (2755) NotI (2804) NheI (2822) BmtI (2826) EcoRI (3617) AscI (3827) SwaI (3930) PasI (4432) XcmI (4542) BstBI (5055) pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS 10,222 bp

Sequence View

5ʹ 3ʹ 60 3' LTR t g g a a g g g c t a a t t c a c t c c c a a a g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a a c c t t c c c g a t t a a g t g a g g g t t t c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t

120 3' LTR c a c a a g g c t a c t t c c c t g a t t a g c a g a a c t a c a c a c c a g g g c c a g g g g t c a g a t a t c c a c g t g t t c c g a t g a a g g g a c t a a t c g t c t t g a t g t g t g g t c c c g g t c c c c a g t c t a t a g g t g

180 3' LTR t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c c a g a t a a g g t a g a a g a g g c c a a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g g t c t a t t c c a t c t t c t c c g g t

240 3' LTR a t a a a g g a g a g a a c a c c a g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g t a t t t c c t c t c t t g t g g t c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c

300 3' LTR a g a g a g a a g t g t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c g t g g c c c g a g t c t c t c t t c a c a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g c a c c g g g c t c

BspEI 360 3' LTR a g c t g c a t c c g g a g t a c t t c a a g a a c t g c t g a t a t c g a g c t t g c t a c a a g g g a c t t t c c g t c g a c g t a g g c c t c a t g a a g t t c t t g a c g a c t a t a g c t c g a a c g a t g t t c c c t g a a a g g c

420 3' LTR c t g g g g a c t t t c c a g g g a g g c g t g g c c t g g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a t g a c c c c t g a a a g g t c c c t c c g c a c c g g a c c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t a

480 3' LTR c c t g c a t a t a a g c a g c t g c t t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g g a c g t a t a t t c g t c g a c g a a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t

540 3' LTR g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a

600 3' LTR t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g

660 3' LTR a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c

720 HIV-1 Ψ c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c

780 HIV-1 Ψ c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t

NruI* 840 HIV-1 Ψ a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c

900 a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c

960 c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a

1020 *I g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a

1080 MIYYLLLGRNHADFSLSLLC c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t

1140 WPLKLCSLPLAFCFYSWRVA c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g

MfeIFseI 1200 MRDNWRS LPRGSIKLGPPPSILSLQLL a a g c g g c c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t t c g c c g g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c

1260 ELYKYKVVKIEPLGVAPTKA SNYLYLTTFISGNPTAGVLA t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g

1320 RRE KRRVVQREKRAVGIGALFLG FLLTTCLSFLATPIPAKNRP a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c

1380 RRE FLGAAGSTMGAASMTLTVQA NKPAAPLVIPAADIVSVTCA g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g

BbvCI 1440 RRE RQLLSGIVQQQNNLLRAIEA LCNNDPITCCCFLKSLAISA c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g

1500 RRE QQHLLQLTVWGIKQLQARIL CCCRNCSVTQPM g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a

1560 RRE AVERYLKDQQLLGIWGCSGK g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t

AleI 1620 LICTTAVPWNASWSNKSLEQ a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t

1680 IWNHTTWMEWDREINNYTSL g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a

1740 1 5 KNEQELL gp41 peptide IHSLIEESQNQQEKNEQELL a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a

1800 10 15 ELDKWASL (in frame with gp41 peptide) WNWFNITNWLWY gp41 peptide ELDKWASLWNWFNITNWLWY g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t

1860 (in frame with gp41 peptide) IKLFIMIVGGLVGLRIVFAV IKLFIMIVGGLVGLRIVFAV t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a

1920 (in frame with gp41 peptide) LSIVNRVRQGYSPLSFQTHL LSIVNRVRQGYSPLSFQTHL a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a

PpuMIKflI 1980 (in frame with gp41 peptide) PTPRGPDRPEGIEEEGGERD PTPRGPDRPEGIEEEGGERD c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t

2040 (in frame with gp41 peptide) RDRSIRLVNGSRRYRQIHKW RDRSIRLVNGSRRYRQIHKW c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g c c a a a t t c a c a a a t g g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c g g t t t a a g t g t t t a c

2100 (in frame with gp41 peptide) QYSSTILKEKGGLGGTVQGK cPPT/CTS QYSSTILKEKGGLGGTVQGK g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t

2160 E* cPPT/CTS E* a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t

SpeI 2220 cPPT/CTS a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a g t t t g g a c t a g t g g a t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t c a a a c c t g a t c a c c t

2280 CMV enhancer g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c

2340 CMV enhancer c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c

2400 CMV enhancer a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t

2460 CMV enhancer t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g

SnaBI 2520 CMV enhancer c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g

2580 CMV enhancer CMV promoter t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g

2640 CMV promoter a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t

2700 CMV promoter C G C A A A T G G G C G G T A G CMV-F MGGR t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c

BsmBIEsp3I 2760 CMV promoter G C G T G CMV-F A G C T C G T T T A G T G A A C C G T C A G A T C LNCX RVRWEVYISRARLVNRQIAW *HVTLDGP g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c g c c t g c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g c g g a c

NotI 2820 RRHPRCFDLHRRHRRPHRSA SAMWATKVEMSSVPPRVPRC g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g g c g g c c g c a c c g g t c t g c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c c g c c g g c g t g g c c a g a c g

BmtINheI 2880 ASMANLGCWMLVLFVATWSD SAHRVKAAPHQNEKHGCPTV a g c t a g c a t g g c g a a c c t t g g c t g c t g g a t g c t g g t t c t c t t t g t g g c c a c a t g g a g t g a t c g a t c g t a c c g c t t g g a a c c g a c g a c c t a c g a c c a a g a g a a a c a c c g g t g t a c c t c a c t

2940 5 10 15 FKNLIWLKEVDSTQER BioID2 LGLCFKNLIWLKEVDSTQER QAEAELVQDPQLLHVAGLLS c c t g g g c c t c t g c t t c a a g a a c c t g a t c t g g c t g a a g g a g g t g g a c a g c a c c c a g g a g a g g g a c c c g g a g a c g a a g t t c t t g g a c t a g a c c g a c t t c c t c c a c c t g t c g t g g g t c c t c t c

3000 20 25 30 35 LKEWNVSYGTALVADRQTKG BioID2 LKEWNVSYGTALVADRQTKG *PVARTASLCVLP QLLPVNAVAGGQHGVSLGLA a c t g a a g g a g t g g a a c g t t a g c t a c g g c a c c g c c c t g g t g g c c g a c a g a c a g a c c a a g g g t g a c t t c c t c a c c t t g c a a t c g a t g c c g t g g c g g g a c c a c c g g c t g t c t g t c t g g t t c c c

3060 40 45 50 55 RGGLGRKWLSQEGGLYFSFL BioID2 RGGLGRKWLSQEGGLYFSFL LPPRPLFHSLWSPPRYKLKR SAAQASLPQALLAAQVEAEQ c a g a g g c g g c c t g g g c a g a a a g t g g c t g a g c c a g g a g g g c g g c c t g t a c t t c a g c t t c c t g t c t c c g c c g g a c c c g t c t t t c a c c g a c t c g g t c c t c c c g c c g g a c a t g a a g t c g a a g g a

3120 60 65 70 75 LNPKEFENLLQLPLVLGLSV BioID2 LNPKEFENLLQLPLVLGLSV SFGLSNSFRSCSGRTSPRLT QVGLLELVQQLQGQHQAQAH g c t g a a c c c c a a g g a g t t c g a g a a c c t g c t g c a g c t g c c c c t g g t g c t g g g c c t g a g c g t c g a c t t g g g g t t c c t c a a g c t c t t g g a c g a c g t c g a c g g g g a c c a c g a c c c g g a c t c g c a

3180 80 85 90 95 SEALEEITEIPFSLKWPNDV BioID2 SEALEEITEIPFSLKWPNDV LSARSSIVSIGKLRFHGLST ALGQLLDGLDGEAQLPGVVH g a g c g a g g c c c t g g a g g a g a t c a c c g a g a t c c c c t t c a g c c t g a a g t g g c c c a a c g a c g t c t c g c t c c g g g a c c t c c t c t a g t g g c t c t a g g g g a a g t c g g a c t t c a c c g g g t t g c t g c a

3240 100 105 110 115 YFQEKKVSGVLCELSKDKLI BioID2 YFQEKKVSGVLCELSKDKLI YKWSFFTLPTSHSSLLSLSI VELLLLHAAHQALQALVLQD g t a c t t c c a g g a g a a g a a g g t g a g c g g c g t g c t g t g c g a g c t g a g c a a g g a c a a g c t g a t c a t g a a g g t c c t c t t c t t c c a c t c g c c g c a c g a c a c g c t c g a c t c g t t c c t g t t c g a c t a

3300 120 125 130 135 VGIGINVNQREIPEEIKDRA BioID2 VGIGINVNQREIPEEIKDRA TPMPMLTFWLSIGSSILSLA HADADVHVLSLDGLLDLVSG c g t g g g c a t c g g c a t c a a c g t g a a c c a g a g a g a g a t c c c c g a g g a g a t c a a g g a c a g a g c g c a c c c g t a g c c g t a g t t g c a c t t g g t c t c t c t c t a g g g g c t c c t c t a g t t c c t g t c t c g

3360 140 145 150 155 TTLYEITGKDWDRKEVLLKV BioID2 TTLYEITGKDWDRKEVLLKV VVRYSIVPLSQSLFSTSSFT GGQVLDGALVPVSLLHQQLH c a c c a c c c t g t a c g a g a t c a c c g g c a a g g a c t g g g a c a g a a a g g a g g t g c t g c t g a a g g t g t g g t g g g a c a t g c t c t a g t g g c c g t t c c t g a c c c t g t c t t t c c t c c a c g a c g a c t t c c a

3420 160 165 170 175 LKRISENLKKFKEKSFKEFK BioID2 LKRISENLKKFKEKSFKEFK SFLILSFRFFNLSFLKLSNL QLSDALVQLLELLLAELLEL g c t g a a g a g a a t c a g c g a g a a c c t g a a g a a g t t c a a g g a g a a g a g c t t c a a g g a g t t c a a c g a c t t c t c t t a g t c g c t c t t g g a c t t c t t c a a g t t c c t c t t c t c g a a g t t c c t c a a g t t

3480 180 185 190 195 GKIESKMLYLGEEVKLLGEG BioID2 GKIESKMLYLGEEVKLLGEG PLISLLISYRPSSTFSSPSP ALDLALHQVQALLHLQQALA g g g c a a g a t c g a g a g c a a g a t g c t g t a c c t g g g c g a g g a g g t g a a g c t g c t g g g c g a g g g c c c g t t c t a g c t c t c g t t c t a c g a c a t g g a c c c g c t c c t c c a c t t c g a c g a c c c g c t c c c

3540 200 205 210 215 KITGKLVGLSEKGGALILTE BioID2 KITGKLVGLSEKGGALILTE LIVPLSTPRLSFPPARIRVS LDGALQHAQALLAAGQDQGL c a a g a t c a c c g g c a a g c t g g t g g g c c t g a g c g a g a a g g g c g g c g c c c t g a t c c t g a c c g a g t t c t a g t g g c c g t t c g a c c a c c c g g a c t c g c t c t t c c c g c c g c g g g a c t a g g a c t g g c t

3600 220 225 230 EGIKEILSGEFSLRRS 1 YPYD BioID2 HA EGIKEILSGEFSLRRSYPYD SPM LADLLDQAALEAQSSAVRVI g g a g g g c a t c a a g g a g a t c c t g a g c g g c g a g t t c a g c c t g a g a a g a a g c t a c c c t t a c g a c c t c c c g t a g t t c c t c t a g g a c t c g c c g c t c a a g t c g g a c t c t t c t t c g a t g g g a a t g c t

EcoRI 3660 5 VPDYA (in frame with HA) EFGSGGGGSGGGGSG HA VPDYAEFGSGGGGSGGGGSG YRIVCFESGTSAPRSAPTTA t g t a c c g g a t t a c g c a g a a t t c g g a t c c g g t g g a g g c g g g t c t g g a g g c g g g g g t a g t g g a c a t g g c c t a a t g c g t c t t a a g c c t a g g c c a c c t c c g c c c a g a c c t c c g c c c c c a t c a c c

3720 (in frame with HA) GGGSGGGGGSGGGGSGGGGS GGGSGGGGGSGGGGSGGGGS PTSAPTSAPRTASTASATST c g g g g g t g g a a g c g g g g g t g g a g g c g g g t c g g g t g g c g g a g g t a g c g g a g g c g g t g g a a g g c c c c c a c c t t c g c c c c c a c c t c c g c c c a g c c c a c c g c c t c c a t c g c c t c c g c c a c c t t c

3780 (in frame with HA) GGGGSGGGGSGGGGSGGGGS GGGGSGGGGSGGGGSGGGGS SATSTTSSTRTASTATASTA t g g a g g c g g t g g a a g t g g t g g a g g a g g t t c t g g t g g c g g a g g t a g c g g t g g c g g a g g t a g a c c t c c g c c a c c t t c a c c a c c t c c t c c a a g a c c a c c g c c t c c a t c g c c a c c g c c t c c a t c

AscI 3840 (in frame with HA) GGGGSGGGGSGGGGSARRSR GGGGSGGGGSGGGGSARRSR SATSTTASTASATSRRATRS c g g a g g c g g t g g a a g t g g t g g c g g a g g t a g c g g a g g c g g t g g a t c g g c g c g c c g t t c t a g g c c t c c g c c a c c t t c a c c a c c g c c t c c a t c g c c t c c g c c a c c t a g c c g c g c g g c a a g a t c

3900 (in frame with HA) KFTIVFPHNQKGNWKNVPSN KFTIVFPHNQKGNWKNVPSN LEGYNKWVVLFSVPFINRRI a a a g t t c a c c a t a g t t t t t c c a c a c a a c c a a a a a g g a a a c t g g a a a a a t g t t c c t t c t a a t t t c a a g t g g t a t c a a a a a g g t g t g t t g g t t t t t c c t t t g a c c t t t t t a c a a g g a a g a t t

SwaI 3960 (in frame with HA) YHYCPSSSDLNWHNDLIGTA YHYCPSSSDLNWHNDLIGTA VM t t a c c a t t a t t g c c c g t c a a g c t c a g a t t t a a a t t g g c a t a a t g a c t t a a t a g g c a c a g c a a t g g t a a t a a c g g g c a g t t c g a g t c t a a a t t t a a c c g t a t t a c t g a a t t a t c c g t g t c g

4020 (in frame with HA) LQVKMPKSHKAIQADGWMCH LQVKMPKSHKAIQADGWMCH c t t a c a a g t c a a a a t g c c c a a g a g t c a c a a g g c t a t t c a a g c a g a c g g t t g g a t g t g t c a g a a t g t t c a g t t t t a c g g g t t c t c a g t g t t c c g a t a a g t t c g t c t g c c a a c c t a c a c a g t

4080 (in frame with HA) ASKWVTTCDFRWYGPKYITQ ASKWVTTCDFRWYGPKYITQ t g c t t c c a a a t g g g t c a c t a c t t g t g a t t t c c g c t g g t a t g g a c c g a a g t a t a t a a c a c a a c g a a g g t t t a c c c a g t g a t g a a c a c t a a a g g c g a c c a t a c c t g g c t t c a t a t a t t g t g t

4140 (in frame with HA) SIRSFTPSVEQCKESIEQTK SIRSFTPSVEQCKESIEQTK g t c c a t c c g a t c c t t c a c t c c a t c t g t a g a a c a a t g c a a g g a a a g c a t t g a a c a a a c g a a c a g g t a g g c t a g g a a g t g a g g t a g a c a t c t t g t t a c g t t c c t t t c g t a a c t t g t t t g c t t

4200 (in frame with HA) QGTWLNPGFPPQSCGYATVT QGTWLNPGFPPQSCGYATVT a c a a g g a a c t t g g c t g a a t c c a g g c t t c c c t c c t c a a a g t t g t g g a t a t g c a a c t g t g a c t g t t c c t t g a a c c g a c t t a g g t c c g a a g g g a g g a g t t t c a a c a c c t a t a c g t t g a c a c t g

4260 (in frame with HA) DAEAVIVQVTPHHVLVDEYT DAEAVIVQVTPHHVLVDEYT g g a t g c c g a a g c a g t g a t t g t c c a g g t g a c t c c t c a c c a t g t g c t g g t t g a t g a a t a c a c c c t a c g g c t t c g t c a c t a a c a g g t c c a c t g a g g a g t g g t a c a c g a c c a a c t a c t t a t g t g

4320 (in frame with HA) GEWVDSQFINGKCSNYICPT GEWVDSQFINGKCSNYICPT a g g a g a a t g g g t t g a t t c a c a g t t c a t c a a c g g a a a a t g c a g c a a t t a c a t a t g c c c c a c t c c t c t t a c c c a a c t a a g t g t c a a g t a g t t g c c t t t t a c g t c g t t a a t g t a t a c g g g g t g

4380 (in frame with HA) VHNSTTWHSDYKVKGLCDSN VHNSTTWHSDYKVKGLCDSN t g t c c a t a a c t c t a c a a c c t g g c a t t c t g a c t a t a a g g t c a a a g g g c t a t g t g a t t c t a a a c a g g t a t t g a g a t g t t g g a c c g t a a g a c t g a t a t t c c a g t t t c c c g a t a c a c t a a g a t t

PasI 4440 (in frame with HA) LISMDITFFSEDGELSSLGK LISMDITFFSEDGELSSLGK c c t c a t t t c c a t g g a c a t c a c c t t c t t c t c a g a g g a c g g a g a g c t a t c a t c c c t g g g a a a g g a g t a a a g g t a c c t g t a g t g g a a g a a g a g t c t c c t g c c t c t c g a t a g t a g g g a c c c t t t

4500 (in frame with HA) EGTGFRSNYFAYETGGKACK EGTGFRSNYFAYETGGKACK g g a g g g c a c a g g g t t c a g a a g t a a c t a c t t t g c t t a t g a a a c t g g a g g c a a g g c c t g c a a c c t c c c g t g t c c c a a g t c t t c a t t g a t g a a a c g a a t a c t t t g a c c t c c g t t c c g g a c g t t

XcmI 4560 (in frame with HA) MQYCKHWGVRLPSGVWFEMA MQYCKHWGVRLPSGVWFEMA a a t g c a a t a c t g c a a g c a t t g g g g a g t c a g a c t c c c a t c a g g t g t c t g g t t c g a g a t g g c t t a c g t t a t g a c g t t c g t a a c c c c t c a g t c t g a g g g t a g t c c a c a g a c c a a g c t c t a c c g

4620 (in frame with HA) DKDLFAAARFPECPEGSSIS DKDLFAAARFPECPEGSSIS t g a t a a g g a t c t c t t t g c t g c a g c c a g a t t c c c t g a a t g c c c a g a a g g g t c a a g t a t c t c a c t a t t c c t a g a g a a a c g a c g t c g g t c t a a g g g a c t t a c g g g t c t t c c c a g t t c a t a g a g

4680 (in frame with HA) APSQTSVDVSLIQDVERILD APSQTSVDVSLIQDVERILD t g c t c c a t c t c a g a c c t c a g t g g a t g t a a g t c t a a t t c a g g a c g t t g a g a g g a t c t t g g a a c g a g g t a g a g t c t g g a g t c a c c t a c a t t c a g a t t a a g t c c t g c a a c t c t c c t a g a a c c t

4740 (in frame with HA) YSLCQETWSKIRAGLPISPV YSLCQETWSKIRAGLPISPV t t a t t c c c t c t g c c a a g a a a c c t g g a g c a a a a t c a g a g c g g g t c t t c c a a t c t c t c c a g t a a t a a g g g a g a c g g t t c t t t g g a c c t c g t t t t a g t c t c g c c c a g a a g g t t a g a g a g g t c a

4800 (in frame with HA) DLSYLAPKNPGTGPAFTIIN DLSYLAPKNPGTGPAFTIIN g g a t c t c a g c t a t c t t g c t c c t a a a a a c c c a g g a a c c g g t c c t g c t t t c a c c a t a a t c a a c c t a g a g t c g a t a g a a c g a g g a t t t t t g g g t c c t t g g c c a g g a c g a a a g t g g t a t t a g t t

4860 (in frame with HA) GTLKYFETRYIRVDIAAPIL GTLKYFETRYIRVDIAAPIL t g g t a c c c t a a a a t a c t t t g a g a c c a g a t a c a t c a g a g t c g a t a t t g c t g c t c c a a t c c t a c c a t g g g a t t t t a t g a a a c t c t g g t c t a t g t a g t c t c a g c t a t a a c g a c g a g g t t a g g a

4920 (in frame with HA) SRMVGMISGTTTERELWDDW SRMVGMISGTTTERELWDDW c t c a a g a a t g g t c g g a a t g a t c a g t g g a a c t a c c a c a g a a a g g g a a c t g t g g g a t g a c t g g a g t t c t t a c c a g c c t t a c t a g t c a c c t t g a t g g t g t c t t t c c c t t g a c a c c c t a c t g a c

4980 (in frame with HA) APYEDVEIGPNGVLRTSSGY APYEDVEIGPNGVLRTSSGY g g c a c c a t a t g a a g a c g t g g a a a t t g g a c c c a a t g g a g t t c t g a g g a c c a g t t c a g g a t a c c g t g g t a t a c t t c t g c a c c t t t a a c c t g g g t t a c c t c a a g a c t c c t g g t c a a g t c c t a t

5040 (in frame with HA) KFPLYMIGHGMLDSDLHLSS KFPLYMIGHGMLDSDLHLSS t a a g t t t c c t t t a t a c a t g a t t g g a c a t g g t a t g t t g g a c t c c g a t c t t c a t c t t a g c t c a t t c a a a g g a a a t a t g t a c t a a c c t g t a c c a t a c a a c c t g a g g c t a g a a g t a g a a t c g a g

BstBI 5100 (in frame with HA) KAQVFEHPHIQDAASQLPDD KAQVFEHPHIQDAASQLPDD a a a g g c t c a g g t g t t c g a a c a t c c t c a c a t t c a a g a c g c t g c t t c g c a a c t t c c t g a t g a t t t c c g a g t c c a c a a g c t t g t a g g a g t g t a a g t t c t g c g a c g a a g c g t t g a a g g a c t a c t

5160 (in frame with HA) ESLFFGDTGLSKNPIELVEG ESLFFGDTGLSKNPIELVEG t g a g a g t t t a t t t t t t g g t g a t a c t g g g c t a t c c a a a a a t c c a a t c g a g c t t g t a g a a g g a c t c t c a a a t a a a a a a c c a c t a t g a c c c g a t a g g t t t t t a g g t t a g c t c g a a c a t c t t c c

5220 (in frame with HA) WFSSWKSSIASSRGSGTTSG WFSSWKSSIASSRGSGTTSG t t g g t t c a g t a g t t g g a a a a g c t c t a t t g c c t c t t c t a g a g g a a g t g g a a c c a c t t c a g g a a c c a a g t c a t c a a c c t t t t c g a g a t a a c g g a g a a g a t c t c c t t c a c c t t g g t g a a g t c c

5280 (in frame with HA) TTRLLSGHTCFTLTGLLGTL TTRLLSGHTCFTLTGLLGTL t a c t a c c c g t c t t c t a t c t g g g c a c a c g t g t t t c a c g t t g a c a g g t t t g c t t g g g a c g c t a t g a t g g g c a g a a g a t a g a c c c g t g t g c a c a a a g t g c a a c t g t c c a a a c g a a c c c t g c g a

PaeR7IPspXIXhoI 5340 (in frame with HA) VTMGLLT* IRES IRES VTMGLLT* a g t a a c c a t g g g c t t g c t g a c t t a g c t c g a g t c t a g a g g a t c c c t c c c c c c c c c c c t a a c t c a t t g g t a c c c g a a c g a c t g a a t c g a g c t c a g a t c t c c t a g g g a g g g g g g g g g g g a t t g

5400 IRES IRES g t t a c t g g c c g a a g c c g c t t g g a a t a a g g c c g g t g t g c g t t t g t c t a t a t g t t a t t t t c c c a a t g a c c g g c t t c g g c g a a c c t t a t t c c g g c c a c a c g c a a a c a g a t a t a c a a t a a a a g g

PspOMIApaI 5460 IRES IRES a c c a t a t t g c c g t c t t t t g g c a a t g t g a g g g c c c g g a a a c c t g g c c c t g t c t t c t t g a c g t g g t a t a a c g g c a g a a a a c c g t t a c a c t c c c g g g c c t t t g g a c c g g g a c a g a a g a a c t g c

5520 IRES IRES G A G A G C G G T T T C C T T A C G IRES reverse a g c a t t c c t a g g g g t c t t t c c c c t c t c g c c a a a g g a a t g c a a g g t c t g t t g a a t g t c g t g t c g t a a g g a t c c c c a g a a a g g g g a g a g c g g t t t c c t t a c g t t c c a g a c a a c t t a c a g c a c

5580 IRES IRES a a g g a a g c a g t t c c t c t g g a a g c t t c t t g a a g a c a a a c a a c g t c t g t a g c g a c c c t t t g c t t c c t t c g t c a a g g a g a c c t t c g a a g a a c t t c t g t t t g t t g c a g a c a t c g c t g g g a a a c g

5640 IRES IRES a g g c a g c g g a a c c c c c c a c c t g g c g a c a g g t g c c t c t g c g g c c a a a a g c c a c g t g t a t a a t c c g t c g c c t t g g g g g g t g g a c c g c t g t c c a c g g a g a c g c c g g t t t t c g g t g c a c a t a t t

PaqCI 5700 IRES IRES g a t a c a c c t g c a a a g g c g g c a c a a c c c c a g t g c c a c g t t g t g a g t t g g a t a g t t g t g g a a c t a t g t g g a c g t t t c c g c c g t g t t g g g g t c a c g g t g c a a c a c t c a a c c t a t c a a c a c c t t

5760 IRES IRES T G G C T C T C C T C A A G C G T A T T IRES-F a g a g t c a a a t g g c t c t c c t c a a g c g t a t t c a a c a a g g g g c t g a a g g a t g c c c a g a a g g t a t c t c a g t t t a c c g a g a g g a g t t c g c a t a a g t t g t t c c c c g a c t t c c t a c g g g t c t t c c a t

5820 IRES IRES c c c c a t t g t a t g g g a t c t g a t c t g g g g c c t c g g t g c a c a t g c t t t a c a t g t g t t t a g t c g g g g g t a a c a t a c c c t a g a c t a g a c c c c g g a g c c a c g t g t a c g a a a t g t a c a c a a a t c a g c

5880 IRES IRES a g g t t a a a a a a a c g t c t a g g c c c c c c g a a c c a c g g g g a c g t g g t t t t c c t t t g a a a a a c a t c c a a t t t t t t t g c a g a t c c g g g g g g c t t g g t g c c c c t g c a c c a a a a g g a a a c t t t t t g t

BstXI 5940 1 5 10 MAQSKHGLTKEMT IRES ZsGreen1 IRES MATHMAQSKHGLTKEMT c g a t g a t a a t a t g g c c a c a c a t a t g g c c c a g t c c a a g c a c g g c c t g a c c a a g g a g a t g a c g c t a c t a t t a t a c c g g t g t g t a t a c c g g g t c a g g t t c g t g c c g g a c t g g t t c c t c t a c t g

6000 15 20 25 30 MKYRMEGCVDGHKFVITGEG ZsGreen1 MKYRMEGCVDGHKFVITGEG c a t g a a g t a c c g c a t g g a g g g c t g c g t g g a c g g c c a c a a g t t c g t g a t c a c c g g c g a g g g g t a c t t c a t g g c g t a c c t c c c g a c g c a c c t g c c g g t g t t c a a g c a c t a g t g g c c g c t c c c

6060 35 40 45 50 IGYPFKGKQAINLCVVEGGP ZsGreen1 IGYPFKGKQAINLCVVEGGP c a t c g g c t a c c c c t t c a a g g g c a a g c a g g c c a t c a a c c t g t g c g t g g t g g a g g g c g g c c c g t a g c c g a t g g g g a a g t t c c c g t t c g t c c g g t a g t t g g a c a c g c a c c a c c t c c c g c c g g g

6120 55 60 65 70 LPFAEDILSAAFMYGNRVFT ZsGreen1 LPFAEDILSAAFMYGNRVFT c t t g c c c t t c g c c g a g g a c a t c t t g t c c g c c g c c t t c a t g t a c g g c a a c c g c g t g t t c a c g a a c g g g a a g c g g c t c c t g t a g a a c a g g c g g c g g a a g t a c a t g c c g t t g g c g c a c a a g t g

Tth111IPflFISalI 6180 75 80 85 90 EYPQDIVDYFKNSCPAGYTW ZsGreen1 EYPQDIVDYFKNSCPAGYTW c g a g t a c c c c c a g g a c a t c g t c g a c t a c t t c a a g a a c t c c t g c c c c g c c g g c t a c a c c t g g c t c a t g g g g g t c c t g t a g c a g c t g a t g a a g t t c t t g a g g a c g g g g c g g c c g a t g t g g a c

6240 95 100 105 110 DRSFLFEDGAVCICNADITV ZsGreen1 DRSFLFEDGAVCICNADITV g g a c c g c t c c t t c c t g t t c g a g g a c g g c g c c g t g t g c a t c t g c a a c g c c g a c a t c a c c g t c c t g g c g a g g a a g g a c a a g c t c c t g c c g c g g c a c a c g t a g a c g t t g c g g c t g t a g t g g c a

6300 115 120 125 130 SVEENCMYHESKFYGVNFPA ZsGreen1 SVEENCMYHESKFYGVNFPA g a g c g t g g a g g a g a a c t g c a t g t a c c a c g a g t c c a a g t t c t a c g g c g t g a a c t t c c c c g c c t c g c a c c t c c t c t t g a c g t a c a t g g t g c t c a g g t t c a a g a t g c c g c a c t t g a a g g g g c g

6360 135 140 145 150 DGPVMKKMTDNWEPSCEKII ZsGreen1 DGPVMKKMTDNWEPSCEKII c g a c g g c c c c g t g a t g a a g a a g a t g a c c g a c a a c t g g g a g c c c t c c t g c g a g a a g a t c a t g c t g c c g g g g c a c t a c t t c t t c t a c t g g c t g t t g a c c c t c g g g a g g a c g c t c t t c t a g t a

6420 155 160 165 170 PVPKQGILKGDVSMYLLLKD ZsGreen1 PVPKQGILKGDVSMYLLLKD c c c c g t g c c c a a g c a g g g c a t c t t g a a g g g c g a c g t g a g c a t g t a c c t g c t g c t g a a g g a g g g g c a c g g g t t c g t c c c g t a g a a c t t c c c g c t g c a c t c g t a c a t g g a c g a c g a c t t c c t

BsrGI 6480 175 180 185 190 GGRLRCQFDTVYKAKSVPRK ZsGreen1 GGRLRCQFDTVYKAKSVPRK c g g t g g c c g c t t g c g c t g c c a g t t c g a c a c c g t g t a c a a g g c c a a g t c c g t g c c c c g c a a g c c a c c g g c g a a c g c g a c g g t c a a g c t g t g g c a c a t g t t c c g g t t c a g g c a c g g g g c g t t

6540 195 200 205 210 MPDWHFIQHKLTREDRSDAK ZsGreen1 MPDWHFIQHKLTREDRSDAK g a t g c c c g a c t g g c a c t t c a t c c a g c a c a a g c t g a c c c g c g a g g a c c g c a g c g a c g c c a a c t a c g g g c t g a c c g t g a a g t a g g t c g t g t t c g a c t g g g c g c t c c t g g c g t c g c t g c g g t t

6600 215 220 225 230 NQKWHLTEHAIASGSALP* ZsGreen1 NQKWHLTEHAIASGSALP* g a a c c a g a a g t g g c a c c t g a c c g a g c a c g c c a t c g c c t c c g g c t c c g c c t t g c c c t g a a t c t t g g t c t t c a c c g t g g a c t g g c t c g t g c g g t a g c g g a g g c c g a g g c g g a a c g g g a c t t a

BspDIClaI 6660 WPRE c g a t a g a t c c t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a g c t a t c t a g g a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t

6720 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R ML MPLYHA a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t

6780 WPRE LLPVWLSFSPPCINPGCCLF IASRMAFIFSSLYKSWLLSL t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a

6840 WPRE MRSCGPLSGNVAWCALCLLT YEELWPVVRQRGVVCTVFAD a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c

6900 WPRE QPPLVGALPPPVSSFPGLSL ATPTGWGIATTCQLLSGTFA c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a

6960 WPRE SPSLLPRRNSSPPALPAAGQ FPLPIATAELIAACLARCWT t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c

7020 WPRE *RGK GLGCWALTIPWCCRGNHRPF GARLLGTDNSVVLSGKSSSF g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g

7080 WPRE (in frame with Factor Xa site) RPQEGTNGGPNQAPRGEAVD LGCSPVLPPGFCAGRPSATS PWLLACVATWILRGTSFCYV c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g

SacII 7140 WPRE R 1 RGEI Factor Xa site LRPSIQRTFLPAACCRLCGL PSALNPADLPSRGLLPALRP c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g

7200 WPRE FRVFAFALRRVGSPFGPPPR LPRLRLRPQTSRISLWAASP t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g

7260 LRSFKTNDLQGSCRS* PEIL* c t g a g a t c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a g a c t c t a g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t

7320 3' LTR ( Δ U3) a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t c t g c t t t t t g c t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a g a c g a a a a a c g

7380 3' LTR ( Δ U3) t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c

7440 3' LTR ( Δ U3) g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c

7500 3' LTR ( Δ U3) t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a

PsiI 7560 3' LTR ( Δ U3) c t c t a g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t a a c t t g c a a a g a a a t g g a g a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a t t g a a c g t t t c t t t a c

PacITspMIXmaISmaI 7620 a a t a t c a g a g a g t g a g a g g c c c g g g t t a a t t a a g g a a a g g g c t a g a t c a t t c t t g a a g a c t t a t a g t c t c t c a c t c t c c g g g c c c a a t t a a t t c c t t t c c c g a t c t a g t a a g a a c t t c t g

7680 C G G A G C A C T A T G C G G A T A A pBRforEco g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a

7740 AmpR promoter a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a

SspI 7800 AmpR promoter a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a

7860 1 5 10 signal sequence MSIQHFRVALIPFF AmpR promoter AmpR MSIQHFRVALIPFF a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c

7920 15 20 signal sequence AAFCLPVFA 25 30 HPETLVKVKDA AmpR AAFCLPVFAHPETLVKVKDA c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c

7980 35 40 45 50 EDQLGARVGYIELDLNSGKI AmpR EDQLGARVGYIELDLNSGKI a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g

8040 55 60 65 70 LESFRPEERFPMMSTFKVLL AmpR C G A T A Amp-R LESFRPEERFPMMSTFKVLL t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a

8100 75 80 85 90 CGAVLSRVDAGQEQLGRRIH AmpR C A C C G C G C C A T A A T A Amp-R CGAVLSRVDAGQEQLGRRIH g t g g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t c a c c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a

8160 95 100 105 110 YSQNDLVEYSPVTEKHLTDG AmpR YSQNDLVEYSPVTEKHLTDG a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t

8220 115 120 125 130 MTVRELCSAAITMSDNTAAN AmpR MTVRELCSAAITMSDNTAAN t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a

PvuI 8280 135 140 145 150 LLLTTIGGPKELTAFLHNMG AmpR LLLTTIGGPKELTAFLHNMG t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c

8340 155 160 165 170 DHVTRLDRWEPELNEAIPND AmpR DHVTRLDRWEPELNEAIPND a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c

FspI 8400 175 180 185 190 ERDTTMPVAMATTLRKLLTG AmpR ERDTTMPVAMATTLRKLLTG a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c

8460 195 200 205 210 ELLTLASRQQLIDWMEADKV AmpR ELLTLASRQQLIDWMEADKV a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c

8520 215 220 225 230 AGPLLRSALPAGWFIADKSG AmpR AGPLLRSALPAGWFIADKSG c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c

8580 235 240 245 250 AGERGSRGIIAALGPDGKPS AmpR AGERGSRGIIAALGPDGKPS c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g

8640 255 260 265 270 RIVVIYTTGSQATMDERNRQ AmpR RIVVIYTTGSQATMDERNRQ g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t

8700 275 280 285 IAEIGASLIKHW* AmpR IAEIGASLIKHW* t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a

8760 a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g

8820 t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c

8880 ori a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a

8940 ori g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g

9000 ori c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g

9060 ori t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c

9120 ori c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a

9180 ori t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a

9240 ori g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g

9300 ori t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t

9360 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t

9420 ori g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c

9480 ori g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a

9540 g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t

9600 A G C G A G T C A G L4440 c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c

9660 T G A G C G A G L4440 t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t

9720 t t c a t t a a t g c a g c a a g c t c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c a a g t a a t t a c g t c g t t c g a g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g

SfiI 9780 c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a

9840 t t g c a a a a a g c t c c c c g t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a a c g t t t t t c g a g g g g c a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t

9900 -35 CAP binding site lac promoter a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g

9960 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G M13 Reverse c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c

10,020 M13 rev A C M13 Reverse G T G G T T T G T EBV-rev a c a t g a t t a c g a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t g t a c t a a t g c t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a

10,080 C C A A A C T C A T C EBV-rev c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g a t c a a c t g g a t a a c t c a a g c t a a c c a a a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g t t g a c c t a t t g a g t t c g a t t g g t t t

10,140 a t c a t c c c a a a c t t c c c a c c c c a t a c c c t a t t a c c a c t g c c a a t t a c c t g t g g t t t c a t t t a g t a g g g t t t g a a g g g t g g g g t a t g g g a t a a t g g t g a c g g t t a a t g g a c a c c a a a g t a a

10,200 t a c t c t a a a c c t g t g a t t c c t c t g a a t t a t t t t c a t t t t a a a g a a a t t g t a t t t g t t a a a a t g a g a t t t g g a c a c t a a g g a g a c t t a a t a a a a g t a a a a t t t c t t t a a c a t a a a c a a t t t

3ʹ 5ʹ 10,222 t a t g t a c t a c a a a c t t a g t a g t a t a c a t g a t g t t t g a a t c a t c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.