Addgene pCMV-XPACK-DNAJC5 (WT) Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCMV-XPACK-DNAJC5 (WT) Sequencing Result


Map View

AseI (4562) PciI (4497) pBR322ori-F (4342 .. 4361) ApaLI (4183) TK-pA-R (3433 .. 3452) PfoI (3354) Neo-F (3115 .. 3134) RsrII (3095) BsrDI (2812) PflFI - Tth111I (2697) FspI (2681) MscI (2661) Neo-R (2505 .. 2524) BspDI * - ClaI * (2419) StuI (2400) SfiI (2354) NdeI (174) SnaBI (280) CMV-F (459 .. 479) NheI (531) BmtI (535) BglII (549) PaeR7I - XhoI (553) Eco53kI (558) SacI (560) EcoRI (569) BamHI (608) AgeI (614) AccI (647) BmgBI (673) SacII (780) PflMI (930) BbsI (943) PmlI (969) BsmBI - Esp3I (1046) PstI (1098) PvuI (1131) MluI (1214) BsiWI (1217) NotI (1223) XbaI * (1233) MfeI (1329) HincII - HpaI (1342) SV40pA-R (1380 .. 1399) BtsI - BtsαI (1418) EBV-rev (1434 .. 1453) AflII (1461) F1ori-F (1608 .. 1629) DraIII (1695) F1ori-R (1820 .. 1839) pBABE 3' (2082 .. 2102) CsiI - SexAI * (2168) pCMV-XPACK-DNAJC5 (WT) 4615 bp

Sequence View

60 CMV enhancer c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a

120 CMV enhancer g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t

NdeI 180 CMV enhancer a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g

240 CMV enhancer a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t

SnaBI 300 CMV enhancer c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g

360 CMV enhancer CMV promoter c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c

420 CMV promoter a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c

480 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a

BmtINheI 540 CMV promoter a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t

EcoRIBglIIPaeR7IXhoIEco53kISacI 600 MGCINSKRK c c g g a c t c a g a t c t c g a g c t c a a g c t t c g a a t t c a t g g g c t g c a t t a a c a g c a a a c g c a a g g c c t g a g t c t a g a g c t c g a g t t c g a a g c t t a a g t a c c c g a c g t a a t t g t c g t t t g c g t t

AccIAgeIBamHI 660 DAGSTGMADQRQRSLSTSGE a g a t g c c g g a t c c a c c g g t a t g g c a g a c c a g a g a c a g c g c t c a c t g t c t a c c t c t g g g g a t c t a c g g c c t a g g t g g c c a t a c c g t c t g g t c t c t g t c g c g a g t g a c a g a t g g a g a c c c c t

BmgBI 720 SLYHVLGLDKNATSDDIKKS *IVNFLG g t c a t t g t a c c a c g t c c t t g g g t t g g a c a a g a a c g c a a c c t c a g a t g a c a t t a a a a a g t c c a g t a a c a t g g t g c a g g a a c c c a a c c t g t t c t t g c g t t g g a g t c t a c t g t a a t t t t t c a g

SacII 780 YRKLALKYHPDKNPDNPEAA IPLKGQFIVGVLVGVVRLGR c t a t c g g a a g c t t g c c t t g a a a t a t c a c c c c g a c a a g a a c c c c g a c a a c c c g g a g g c c g c g a t a g c c t t c g a a c g g a a c t t t a t a g t g g g g c t g t t c t t g g g g c t g t t g g g c c t c c g g c g

840 DKFKEINNAHAILTDATKRN VLKLLDVVRVGDERVGCFPV g g a c a a g t t t a a g g a g a t c a a c a a c g c g c a c g c c a t c c t c a c g g a c g c c a c a a a a a g g a a c c t g t t c a a a t t c c t c t a g t t g t t g c g c g t g c g g t a g g a g t g c c t g c g g t g t t t t t c c t t

900 IYDKYGSLGLYVAEQFGEEN DVVLVARQTEVHGLLKPFLV c a t c t a c g a c a a g t a c g g c t c g c t g g g t c t c t a c g t g g c c g a g c a g t t t g g g g a a g a g a a g t a g a t g c t g t t c a t g c c g a g c g a c c c a g a g a t g c a c c g g c t c g t c a a a c c c c t t c t c t t

BbsIPflMI 960 VNTYFVLSSWWAKALFVFCG HVGVEHQGAPPGLGQKDEAA c g t g a a c a c c t a c t t c g t g c t g t c c a g c t g g t g g g c c a a g g c c c t g t t t g t c t t c t g c g g g c a c t t g t g g a t g a a g c a c g a c a g g t c g a c c a c c c g g t t c c g g g a c a a a c a g a a g a c g c c

PmlI 1020 LLTCCYCCCCLCCCFNCCCG EERAAVAAATQAAAEVAAAP c c t c c t c a c g t g c t g c t a c t g c t g c t g c t g t c t g t g c t g c t g c t t c a a c t g c t g c t g c g g g g a g g a g t g c a c g a c g a t g a c g a c g a c g a c a g a c a c g a c g a c g a a g t t g a c g a c g a c g c c

BsmBIEsp3I 1080 KCKPKAPEGEETEFYVSPED LTLGLRRFALLRLEVHGGLI g a a g t g t a a g c c c a a g g c g c c t g a a g g c g a g g a g a c g g a g t t c t a c g t g t c c c c c g a g g a c t t c a c a t t c g g g t t c c g c g g a c t t c c g c t c c t c t g c c t c a a g a t g c a c a g g g g g c t c c t

PvuIPstI 1140 LEAQLQSDEREATDTPIVIQ QLCLQLRVLPLGCVRRDDYL t c t g g a g g c a c a g c t g c a g t c t g a c g a g a g g g a g g c c a c a g a c a c g c c g a t c g t c a t a c a a g a c c t c c g t g t c g a c g t c a g a c t g c t c t c c c t c c g g t g t c t g t g c g g c t a g c a g t a t g t

1200 PASATETTQLTADSHPSYHT RCGGGLGGLECGVGVGAVVS g c c g g c a t c c g c c a c c g a g a c c a c c c a g c t c a c a g c c g a c t c c c a c c c c a g c t a c c a c a c c g g c c g t a g g c g g t g g c t c t g g t g g g t c g a g t g t c g g c t g a g g g t g g g g t c g a t g g t g t g

XbaI*NotIMluIBsiWI 1260 DGFNTRTRPRL* VPEVRTRPRSELDYDAM t g a c g g g t t c a a c a c g c g t a c g c g g c c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t a c t g c c c a a g t t g t g c g c a t g c g c c g g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a

1320 t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t

HincIIHpaIMfeI 1380 SV40 poly(A) signal C SV40pA-R a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c

BtsαIBtsI 1440 SV40 poly(A) signal G T G G T T T EBV-rev G T T A T C G T A G T G T T T A A A G SV40pA-R c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a

AflII 1500 SV40 poly(A) signal f1 ori G T C C A A A C T C A T C EBV-rev g t c c a a a c t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t c a g g t t t g a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a

1560 f1 ori t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t

1620 f1 ori G G T C A A A C C T T G T F1ori-F t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t

1680 f1 ori T C T C A G G T G F1ori-F a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c

DraIII 1740 f1 ori g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t

1800 f1 ori a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c

1860 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t

1920 f1 ori g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c

1980 f1 ori AmpR promoter g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a

2040 AmpR promoter a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a

2100 AmpR promoter SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C pBABE 3' a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c

2160 SV40 promoter SV40 enhancer C A pBABE 3' g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t

SexAI*CsiI 2220 SV40 promoter SV40 enhancer g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a

2280 SV40 promoter SV40 enhancer SV40 ori g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g

2340 SV40 promoter SV40 ori t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t

StuISfiI 2400 SV40 promoter SV40 ori g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c

BspDI*ClaI* 2460 1 MIE SV40 promoter NeoR/KanR 1 MIE SV40 ori NeoR/KanR MIE c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g

2520 5 10 15 20 QDGLHAGSPAAWVERLFGYD NeoR/KanR 5 10 15 20 QDGLHAGSPAAWVERLFGYD NeoR/KanR G A T A A G C C G A T A C T G A Neo-R QDGLHAGSPAAWVERLFGYD a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a

2580 25 30 35 40 WAQQTIGCSDAAVFRLSAQG NeoR/KanR 25 30 35 40 WAQQTIGCSDAAVFRLSAQG NeoR/KanR C C C G Neo-R WAQQTIGCSDAAVFRLSAQG g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g

2640 45 50 55 60 RPVLFVKTDLSGALNELQDE NeoR/KanR 45 50 55 60 RPVLFVKTDLSGALNELQDE NeoR/KanR RPVLFVKTDLSGALNELQDE g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c

Tth111IPflFIFspIMscI 2700 65 70 75 80 AARLSWLATTGVPCAAVLDV NeoR/KanR 65 70 75 80 AARLSWLATTGVPCAAVLDV NeoR/KanR AARLSWLATTGVPCAAVLDV c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c

2760 85 90 95 100 VTEAGRDWLLLGEVPGQDLL NeoR/KanR 85 90 95 100 VTEAGRDWLLLGEVPGQDLL NeoR/KanR VTEAGRDWLLLGEVPGQDLL t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a

BsrDI 2820 105 110 115 120 SSHLAPAEKVSIMADAMRRL NeoR/KanR 105 110 115 120 SSHLAPAEKVSIMADAMRRL NeoR/KanR SSHLAPAEKVSIMADAMRRL *RVKSRGLFYGDHSICHPPQ c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g

2880 125 130 135 140 HTLDPATCPFDHQAKHRIER NeoR/KanR 125 130 135 140 HTLDPATCPFDHQAKHRIER NeoR/KanR HTLDPATCPFDHQAKHRIER MRKIRSGAWEVVLRFMADLS a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c

2940 145 150 155 160 ARTRMEAGLVDQDDLDEEHQ NeoR/KanR 145 150 155 160 ARTRMEAGLVDQDDLDEEHQ NeoR/KanR ARTRMEAGLVDQDDLDEEHQ CTSPHFGTKDILIIQVFLML c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c

3000 165 170 175 180 GLAPAELFARLKASMPDGED NeoR/KanR 165 170 175 180 GLAPAELFARLKASMPDGED NeoR/KanR GLAPAELFARLKASMPDGED PERWGFQEGPELRAHGVALI g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a g

3060 185 190 195 200 LVVTHGDACLPNIMVENGRF NeoR/KanR 185 190 195 200 LVVTHGDACLPNIMVENGRF NeoR/KanR LVVTHGDACLPNIMVENGRF EDHGMAIGAQRIDHHFIAAK t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a

RsrII 3120 205 210 215 220 SGFIDCGRLGVADRYQDIAL NeoR/KanR 205 210 215 220 SGFIDCGRLGVADRYQDIAL NeoR/KanR C G T T G G Neo-F SGFIDCGRLGVADRYQDIAL RSEDVTAPQTHRVAILVYRQ c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c

3180 225 230 235 240 ATRDIAEELGGEWADRFLVL NeoR/KanR 225 230 235 240 ATRDIAEELGGEWADRFLVL NeoR/KanR C T A C C C G T G A T A T T Neo-F ATRDIAEELGGEWADRFLVL SGTINSFLKAAFPSVAEEHK c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a

3240 245 250 255 260 YGIAAPDSQRIAFYRLLDEF NeoR/KanR 245 250 255 260 YGIAAPDSQRIAFYRLLDEF NeoR/KanR YGIAAPDSQRIAFYRLLDEF VTDGSGIRLADGEIAKKVLE a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a

3300 265 F* NeoR/KanR 265 F* NeoR/KanR MTDQATPNLPSR F* ESRSEPTRFSRGLSAWGAM t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c

PfoI 3360 DFDSTAAFYERLGFGIVFRD a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t

3420 AGWMILQRGDLMLEFFAHPR c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c t a g g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g a t c

3480 HSV TK poly(A) signal A C T T T G T G C C T T C C T C T G T T TK-pA-R GRLTETRKETIPEGTRAMTA g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a c c c g c g c t a t g a c g g c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t g g g c g c g a t a c t g c c g

3540 HSV TK poly(A) signal IKRQNKTHGVGSFVHKRGVR a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c

3600 SQGWHSVDTPPRPHWGQYAR g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t g g g g c c a a t a c g c c c g c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a c c c c g g t t a t g c g g g c

3660 VSSFSPPHPPSSGEGPGLAA c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t c g

3720 NVGAAGPAIASGYSYIL* c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t a t a t a c t t t a g a t t g a g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a t a t a t g a a a t c t a a c t

3780 t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a

3840 g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a

3900 ori c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t

3960 ori a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t

4020 ori g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a

4080 ori a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a

4140 ori a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t

ApaLI 4200 ori g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a

4260 ori g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g

4320 ori g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t

4380 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c

4440 ori c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t

PciI 4500 ori a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a

4560 g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a t g c a t t a g t t c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a a t c a a

AseI 4615 a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.