Addgene pCDNA3.1-HsPcdh21-Myc-Flag-His Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCDNA3.1-HsPcdh21-Myc-Flag-His Sequencing Result


Map View

SgrDI (8164) SspI (8047) Amp-R (7793 .. 7812) PvuI (7613) AhdI (7243) pBR322ori-F (6491 .. 6510) L4440 (6240 .. 6257) CAP binding site lac promoter M13/pUC Reverse (6022 .. 6044) lac operator M13 Reverse (6009 .. 6025) M13 rev BstZ17I (5971) EBV-rev (5930 .. 5949) SV40pA-R (5876 .. 5895) PfoI (5774) Neo-F (5535 .. 5554) RsrII (5515) BssHII (5396) PflFI - Tth111I (5117) MscI (5081) PstI (5052) PluTI (5002) SfoI (5000) NarI (4999) KasI (4998) Neo-R (4925 .. 4944) SmaI (4812) TspMI - XmaI (4810) AvrII (4789) SV40pro-F (4717 .. 4736) pBABE 3' (4470 .. 4490) F1ori-F (4330 .. 4351) DraIII (4266) F1ori-R (4120 .. 4139) BglII (12) pRS-marker (44 .. 63) NruI (208) MluI (228) SpeI (249) NdeI (484) CMV-F (769 .. 789) T7 (863 .. 882) T7 promoter NheI (895) BmtI (899) AflII (908) Acc65I (917) KpnI (921) PaqCI (924) BsiWI (1338) BsmBI - Esp3I (1805) BbvCI (2601) PflMI (3093) SgrAI (3332) TEV site Myc 3xFLAG 10xHis NotI (3714) PaeR7I - PspXI - XhoI (3720) PspOMI (3732) ApaI (3736) BGH-rev (3757 .. 3774) pCDNA3.1-HsPcdh21-Myc-Flag-His 8166 bp

Sequence View

BglII 5ʹ 3ʹ 60 A T G T T A G A C G A G A C T A C pRS-marker g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c

120 G G C pRS-marker c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c

180 c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g

MluINruI 240 CMV enhancer t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a

SpeI 300 CMV enhancer g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t

360 CMV enhancer t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g

420 CMV enhancer c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g

480 CMV enhancer a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a

NdeI 540 CMV enhancer a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a

600 CMV enhancer a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t

660 CMV enhancer CMV promoter t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c

720 CMV promoter a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g

780 CMV promoter C G C A A A T G G G C G CMV-F a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c

840 CMV promoter G T A G G C G T G CMV-F g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t

BmtINheI 900 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g c g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a c c g a t c g

Acc65IKpnIPaqCIAflII 960 MRRCRWAALALGL g t t t a a a c t t a a g c t t g g t a c c a t g c g g c g g t g c a g g t g g g c a g c a t t a g c c c t g g g t c t c a a a t t t g a a t t c g a a c c a t g g t a c g c c g c c a c g t c c a c c c g t c g t a a t c g g g a c c c a g a

1020 LRLCLAQANFAPHFFDNGVG t c t a c g a t t g t g t t t g g c c c a a g c t a a t t t t g c t c c g c a c t t t t t c g a t a a c g g g g t t g g a g a t g c t a a c a c a a a c c g g g t t c g a t t a a a a c g a g g c g t g a a a a a g c t a t t g c c c c a a c c

1080 STNGNMALFSLPEDTPVGSH g a g t a c a a a t g g t a a t a t g g c c c t g t t c a g c t t a c c t g a g g a t a c g c c g g t t g g t t c t c a c t c a t g t t t a c c a t t a t a c c g g g a c a a g t c g a a t g g a c t c c t a t g c g g c c a a c c a a g a g t

1140 VYTLNGTDPEGDPISYHISF c g t t t a t a c g t t a a a c g g t a c t g a t c c t g a g g g t g a t c c a a t a t c c t a t c a t a t t t c g t t g c a a a t a t g c a a t t t g c c a t g a c t a g g a c t c c c a c t a g g t t a t a g g a t a g t a t a a a g c a a

1200 DPSTRSVFSVDPTFGNITLV t g a t c c g t c c a c g c g g t c a g t c t t t t c g g t c g a c c c c a c a t t t g g t a a c a t c a c t c t c g t a c t a g g c a g g t g c g c c a g t c a g a a a a g c c a g c t g g g g t g t a a a c c a t t g t a g t g a g a g c a

1260 EELDREREDEIEAIISISDG t g a g g a a c t a g a c c g g g a g c g t g a g g a c g a g a t c g a a g c g a t a a t a t c c a t c t c t g a t g g a c t c c t t g a t c t g g c c c t c g c a c t c c t g c t c t a g c t t c g c t a t t a t a g g t a g a g a c t a c c

1320 LNLVAEKVVILVTDANDEAP a t t a a a t t t g g t a g c a g a a a a g g t g g t t a t t c t g g t a a c c g a c g c t a a t g a c g a g g c t c c t a a t t t a a a c c a t c g t c t t t t c c a c c a a t a a g a c c a t t g g c t g c g a t t a c t g c t c c g a g g

BsiWI 1380 RFIQEPYVALVPEDIPAGSI t a g a t t c a t a c a a g a g c c g t a c g t t g c t c t c g t a c c a g a a g a c a t a c c a g c g g g a t c a a t a t c t a a g t a t g t t c t c g g c a t g c a a c g a g a g c a t g g t c t t c t g t a t g g t c g c c c t a g t t a

1440 IFKVHAVDRDTGSGGSVTYF t a t c t t c a a a g t c c a t g c t g t c g a c c g a g a t a c t g g a t c g g g t g g g t c t g t c a c c t a t t t a t a g a a g t t t c a g g t a c g a c a g c t g g c t c t a t g a c c t a g c c c a c c c a g a c a g t g g a t a a a

1500 LQNLHSPFAVDRHSGVLRLQ c c t a c a g a a t c t c c a t a g t c c t t t t g c g g t a g a t c g t c a t a g t g g c g t c c t c a g a c t c c a g g a t g t c t t a g a g g t a t c a g g a a a a c g c c a t c t a g c a g t a t c a c c g c a g g a g t c t g a g g t

1560 AGATLDYERSRTHYITVVAK a g c g g g a g c t a c c t t g g a c t a c g a a a g g t c c c g c a c c c a c t a t a t a a c c g t t g t c g c a a a t c g c c c t c g a t g g a a c c t g a t g c t t t c c a g g g c g t g g g t g a t a t a t t g g c a a c a g c g t t t

1620 DGGGRLHGADVVFSATTTVT a g a c g g g g g t g g g a g g c t g c a c g g c g c a g a c g t g g t a t t t t c a g c c a c a a c c a c g g t c a c t c t g c c c c c a c c c t c c g a c g t g c c g c g t c t g c a c c a t a a a a g t c g g t g t t g g t g c c a g t g

1680 VNVEDVQDMAPVFVGTPYYG t g t g a a c g t g g a g g a c g t a c a g g a t a t g g c c c c g g t a t t t g t g g g c a c a c c g t a t t a c g g a c a c t t g c a c c t c c t g c a t g t c c t a t a c c g g g g c c a t a a a c a c c c g t g t g g c a t a a t g c c

1740 YVYEDTLPGSEVLKVVAMDG g t a t g t t t a t g a a g a c a c c t t a c c a g g t t c c g a a g t a c t c a a g g t g g t t g c t a t g g a t g g c a t a c a a a t a c t t c t g t g g a a t g g t c c a a g g c t t c a t g a g t t c c a c c a a c g a t a c c t a c c

1800 DRGKPNRILYSLVNGNDGAF a g a t a g a g g c a a a c c g a a t c g c a t t c t t t a c t c a c t c g t g a a t g g c a a c g a c g g g g c a t t t c t a t c t c c g t t t g g c t t a g c g t a a g a a a t g a g t g a g c a c t t a c c g t t g c t g c c c c g t a a

BsmBIEsp3I 1860 EINETSGAISITQSPAQLQR c g a g a t t a a c g a g a c g t c g g g g g c g a t a t c g a t c a c c c a a a g t c c a g c a c a g c t t c a a a g g c t c t a a t t g c t c t g c a g c c c c c g c t a t a g c t a g t g g g t t t c a g g t c g t g t c g a a g t t t c

1920 EVYELHVQVTEMSPAGSPAA a g a a g t c t a c g a a t t a c a t g t t c a a g t a a c t g a g a t g a g c c c t g c g g g c t c g c c t g c t g c t c t t c a g a t g c t t a a t g t a c a a g t t c a t t g a c t c t a c t c g g g a c g c c c g a g c g g a c g a c g

1980 QATVPVTIRIVDLNNHPPTF g c a a g c t a c c g t c c c g g t g a c a a t t a g a a t c g t g g a c c t g a a c a a c c a t c c c c c g a c g t t c g t t c g a t g g c a g g g c c a c t g t t a a t c t t a g c a c c t g g a c t t g t t g g t a g g g g g c t g c a a

2040 YGESGPQNRFELSMNEHPPQ t t a c g g a g a a a g t g g a c c a c a g a a t a g a t t t g a a t t g a g t a t g a a t g a a c a c c c c c c g c a a a t g c c t c t t t c a c c t g g t g t c t t a t c t a a a c t t a a c t c a t a c t t a c t t g t g g g g g g c g t

2100 GEILRGLKITVNDSDQGANA a g g a g a g a t t c t g c g a g g c c t c a a a a t t a c g g t a a a t g a t t c a g a c c a g g g a g c c a a t g c t c c t c t c t a a g a c g c t c c g g a g t t t t a a t g c c a t t t a c t a a g t c t g g t c c c t c g g t t a c g

2160 KFNLQLVGPRGIFRVVPQTV g a a g t t t a a t c t t c a a c t t g t c g g g c c g c g a g g t a t t t t t c g t g t a g t a c c a c a g a c t g t c t t c a a a t t a g a a g t t g a a c a g c c c g g c g c t c c a t a a a a a g c a c a t c a t g g t g t c t g a c a

2220 LNEAQVTIIVENSAAIDFEK g c t g a a c g a g g c c c a g g t c a c c a t t a t a g t c g a a a a t a g c g c g g c c a t c g a t t t c g a a a a c g a c t t g c t c c g g g t c c a g t g g t a a t a t c a g c t t t t a t c g c g c c g g t a g c t a a a g c t t t t

2280 SKVLTFKLLAVEVNTPEKFS a t c a a a g g t g c t c a c a t t c a a g t t a t t a g c g g t a g a a g t a a a t a c g c c g g a a a a a t t c t c t a g t t t c c a c g a g t g t a a g t t c a a t a a t c g c c a t c t t c a t t t a t g c g g c c t t t t t a a g a g

2340 STADVVIQLLDTNDNVPKFD t t c g a c g g c t g a c g t c g t g a t t c a a c t t t t g g a c a c a a a c g a t a a c g t a c c t a a a t t t g a a a g c t g c c g a c t g c a g c a c t a a g t t g a a a a c c t g t g t t t g c t a t t g c a t g g a t t t a a a c t

2400 SLYYVARIPENAPGGSSVVA c t c g c t g t a t t a c g t g g c g a g g a t a c c t g a a a a c g c t c c a g g c g g a t c t t c a g t g g t a g c g a g c g a c a t a a t g c a c c g c t c c t a t g g a c t t t t g c g a g g t c c g c c t a g a a g t c a c c a t c g

2460 VTAVDPDTGPWGEVKYSTYG c g t t a c a g c g g t t g a c c c a g a c a c g g g a c c c t g g g g g g a g g t g a a g t a c a g c a c c t a t g g g c a a t g t c g c c a a c t g g g t c t g t g c c c t g g g a c c c c c c t c c a c t t c a t g t c g t g g a t a c c

2520 TGADLFLIHPSTGLIYTQPW c a c g g g c g c g g a t c t c t t c t t g a t t c a c c c g a g t a c c g g c t t a a t c t a t a c g c a a c c t t g g t g c c c g c g c c t a g a g a a g a a c t a a g t g g g c t c a t g g c c g a a t t a g a t a t g c g t t g g a a c

2580 ASLDAEATARYNFYVKAEDM g g c t t c t c t a g a c g c c g a a g c c a c t g c a c g a t a c a a c t t c t a c g t a a a a g c g g a a g a c a t c c g a a g a g a t c t g c g g c t t c g g t g a c g t g c t a t g t t g a a g a t g c a t t t t c g c c t t c t g t a

BbvCI 2640 EGKYSVAEVFITLLDVNDHP g g a a g g g a a g t a c t c a g t g g c t g a g g t g t t c a t t a c c c t a c t c g a t g t t a a t g a c c a c c c c c t t c c c t t c a t g a g t c a c c g a c t c c a c a a g t a a t g g g a t g a g c t a c a a t t a c t g g t g g g

2700 PQFGKSVQKKTMVLGTPVKI t c c c c a g t t c g g a a a g a g c g t g c a a a a a a a a a c a a t g g t t c t a g g a a c t c c t g t t a a g a t a g g g g t c a a g c c t t t c t c g c a c g t t t t t t t t t g t t a c c a a g a t c c t t g a g g a c a a t t c t a

2760 EAIDEDAEEPNNLVDYSITH a g a a g c a a t t g a t g a a g a t g c t g a a g a a c c c a a c a a t c t a g t t g a t t a c t c g a t c a c g c a t c t t c g t t a a c t a c t t c t a c g a c t t c t t g g g t t g t t a g a t c a a c t a a t g a g c t a g t g c g t

2820 AEPANVFDINSHTGEIWLKN c g c g g a g c c a g c a a a t g t a t t t g a t a t c a a c a g t c a c a c a g g g g a a a t a t g g c t g a a a a a g c g c c t c g g t c g t t t a c a t a a a c t a t a g t t g t c a g t g t g t c c c c t t t a t a c c g a c t t t t t

2880 SIRSLDALHNITPGRDCLWS t a g t a t a a g g t c c c t c g a t g c a c t a c a t a a t a t a a c a c c c g g a c g t g a c t g t c t a t g g t c a t c a t a t t c c a g g g a g c t a c g t g a t g t a t t a t a t t g t g g g c c t g c a c t g a c a g a t a c c a g

2940 LEVQAKDRGSPSFSTTALLK c c t t g a g g t c c a g g c c a a a g a t c g c g g c t c t c c a t c c t t c t c t a c a a c t g c a c t g c t g a a g g a a c t c c a g g t c c g g t t t c t a g c g c c g a g a g g t a g g a a g a g a t g t t g a c g t g a c g a c t t

3000 IDITDAETLSRSPMAAFLIQ g a t a g a c a t c a c t g a t g c a g a g a c t c t t t c g c g c t c a c c t a t g g c t g c c t t t t t a a t a c a c t a t c t g t a g t g a c t a c g t c t c t g a g a a a g c g c g a g t g g a t a c c g a c g g a a a a a t t a t g t

3060 TKDNPMKAVGVLAGTMATVV g a c t a a g g a t a a c c c c a t g a a a g c g g t c g g c g t t t t g g c c g g t a c t a t g g c a a c c g t t g t c t g a t t c c t a t t g g g g t a c t t t c g c c a g c c g c a a a a c c g g c c a t g a t a c c g t t g g c a a c a

PflMI 3120 AITVLISTATFWRNKKSNKV g g c g a t c a c a g t t t t g a t c a g t a c a g c c a c t t t c t g g c g g a a c a a g a a a a g c a a c a a g g t c c g c t a g t g t c a a a a c t a g t c a t g t c g g t g a a a g a c c g c c t t g t t c t t t t c g t t g t t c c a

3180 LPMRRVLRKRPSPAPRTIRI a t t g c c g a t g c g g c g t g t c t t a c g c a a g a g g c c g a g t c c t g c a c c c c g a a c t a t t c g t a t t a a c g g c t a c g c c g c a c a g a a t g c g t t c t c c g g c t c a g g a c g t g g g g c t t g a t a a g c a t a

3240 EWLKSKSTKAATKFMLKEKP c g a g t g g c t c a a g t c a a a a t c t a c g a a g g c a g c c a c a a a g t t c a t g t t a a a g g a g a a g c c g c t c a c c g a g t t c a g t t t t a g a t g c t t c c g t c g g t g t t t c a a g t a c a a t t t c c t c t t c g g

3300 PNENCNNNSPESSLLPRAPA c c c a a a c g a a a a t t g c a a c a a c a a c t c t c c t g a g t c a t c c c t a c t a c c a a g a g c t c c a g c g g g t t t g c t t t t a a c g t t g t t g t t g a g a g g a c t c a g t a g g g a t g a t g g t t c t c g a g g t c g

SgrAI 3360 LPPPPSVAPSTGAAQWTVPT c t t g c c c c c g c c c c c c t c g g t t g c a c c c a g c a c c g g c g c t g c a c a g t g g a c t g t a c c t a c g a a c g g g g g c g g g g g g a g c c a a c g t g g g t c g t g g c c g c g a c g t g t c a c c t g a c a t g g a t g

3420 VSGSLTPQPTQPPPKPKTMG g g t c a g c g g a a g t c t a a c g c c g c a g c c t a c c c a g c c t c c c c c t a a g c c t a a g a c g a t g g g c c a g t c g c c t t c a g a t t g c g g c g t c g g a t g g g t c g g a g g g g g a t t c g g a t t c t g c t a c c c

3480 SPVQSTLISELKQKFEKKSV g t c t c c a g t t c a a a g c a c a c t g a t c t c t g a g c t a a a a c a a a a a t t c g a g a a a a a g t c t g t c a g a g g t c a a g t t t c g t g t g a c t a g a g a c t c g a t t t t g t t t t t a a g c t c t t t t t c a g a c a

3540 1 5 ENLYFQG GG TEV site HNKAYFGGGGSENLYFQGGG g c a c a a t a a g g c c t a t t t t g g t g g t g g t g g a a g t g a a a a t c t t t a t t t t c a a g g t g g a g g c g t g t t a t t c c g g a t a a a a c c a c c a c c a c c t t c a c t t t t a g a a a t a a a a g t t c c a c c t c c

3600 GGSA 1 5 10 EQKLISEEDL (in frame with Myc) GGGGS 1 D Myc 3xFLAG GGSAEQKLISEEDLGGGGSD g g g g g g c t c c g c a g a g c a g a a g c t t a t a a g c g a g g a g g a c c t t g g c g g c g g t g g g t c c g a c c c c c c g a g g c g t c t c g t c t t c g a a t a t t c g c t c c t c c t g g a a c c g c c g c c a c c c a g g c t

3660 5 10 15 20 YKDHDGDYKDHDIDYKDDDD 3xFLAG YKDHDGDYKDHDIDYKDDDD c t a c a a a g a c c a t g a t g g c g a t t a t a a a g a t c a t g a c a t t g a t t a t a a a g a c g a t g a t g a g a t g t t t c t g g t a c t a c c g c t a a t a t t t c t a g t a c t g t a a c t a a t a t t t c t g c t a c t a c t

NotI 3720 K (in frame with 3xFLAG) GGGGS 1 5 10 HHHHHHHHHH * 3xFLAG 10xHis KGGGGSHHHHHHHHHH* c a a a g g a g g t g g t g g g t c c c a t c a t c a c c a t c a c c a t c a t c a t c a c c a c t g a g c g g c c g c g t t t c c t c c a c c a c c c a g g g t a g t a g t g g t a g t g g t a g t a g t a g t g g t g a c t c g c c g g c g

PspOMIApaIPaeR7IPspXIXhoI 3780 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev t c g a g t c t a g a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c t c a g a t c t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g

3840 bGH poly(A) signal a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t

3900 bGH poly(A) signal c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t

3960 bGH poly(A) signal t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g

4020 bGH poly(A) signal a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t

4080 f1 ori g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g

4140 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a

4200 f1 ori c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c

4260 f1 ori g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a

DraIII 4320 f1 ori c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a

4380 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a

4440 f1 ori c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a

4500 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g

4560 SV40 promoter c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c

4620 SV40 promoter g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t

4680 SV40 promoter SV40 ori g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g

4740 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g

AvrII 4800 SV40 promoter SV40 ori c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c

TspMIXmaISmaI 4860 SV40 promoter c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a c a g g a t g a g g g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t g t c c t a c t c c

4920 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR MIEQDGLHAGSPAAWVE a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t

4980 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R RLFGYDWAQQTIGCSDAAVF g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a

KasINarISfoIPluTI 5040 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR RLSAQGRPVLFVKTDLSGAL c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a

MscIPstI 5100 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR NELQDEAARLSWLATTGVPC g a a t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c t t a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c

Tth111IPflFI 5160 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR AAVLDVVTEAGRDWLLLGEV c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a

5220 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR PGQDLLSSHLAPAEKVSIMA *RVKSRGLFYGDHS g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g

5280 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR DAMRRLHTLDPATCPFDHQA ICHPPQMRKIRSGAWEVVLR t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g

5340 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR KHRIERARTRMEAGLVDQDD FMADLSCTSPHFGTKDILII g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t

BssHII 5400 160 165 170 175 LDEEHQGLAPAELFARLKAR NeoR/KanR LDEEHQGLAPAELFARLKAR QVFLMLPERWGFQEGPELRA t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c

5460 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR MPDGEDLVVTHGDACLPNIM HGVALIEDHGMAIGAQRIDH c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a

RsrII 5520 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR VENGRFSGFIDCGRLGVADR HFIAAKRSEDVTAPQTHRVA g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c

5580 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F YQDIALATRDIAEELGGEWA ILVYRQSGTINSFLKAAFPS c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g

5640 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR DRFLVLYGIAAPDSQRIAFY VAEEHKVTDGSGIRLADGEI t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t

5700 260 265 RLLDEFF* NeoR/KanR RLLDEFF* AKKVLEESRSEPTRFSRGLS t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c

5760 AWGAM a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g

PfoI 5820 t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c

5880 SV40 poly(A) signal C G T T A SV40pA-R g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a

5940 SV40 poly(A) signal G T G G T T T G T C C EBV-rev T C G T A G T G T T T A A A G SV40pA-R a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g

BstZ17I 6000 SV40 poly(A) signal A A A C T C A T C EBV-rev a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c

6060 -10 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g

6120 -10 -35 lac promoter CAP binding site a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t

6180 CAP binding site t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a

6240 G L4440 t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g

6300 A G C G A G T G A C T G A G C G A L4440 t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t

6360 a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t

6420 ori a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c

6480 ori c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c

6540 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a

6600 ori c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a

6660 ori t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g

6720 ori t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a

6780 ori g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a

6840 ori a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g

6900 ori t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t

6960 ori a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a

7020 ori t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a

7080 a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t

7140 t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t

7200 285 280 *WHKILSAGI AmpR *WHKILSAGI a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g

AhdI 7260 275 270 265 260 EAIQRNREDMTAQSGTTYIV AmpR EAIQRNREDMTAQSGTTYIV t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a

7320 255 250 245 240 VIRSPKGDPGLAAIIGRSGR AmpR VIRSPKGDPGLAAIIGRSGR a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g

7380 235 230 225 220 EGAGSKDAIFWGAPLASRLL AmpR EGAGSKDAIFWGAPLASRLL t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a

7440 215 210 205 200 PGAVKDAEMWDILQQRSALT AmpR PGAVKDAEMWDILQQRSALT g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t

7500 195 190 185 180 LLEGTLLKRLTTAMAVPMTT AmpR LLEGTLLKRLTTAMAVPMTT a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c

7560 175 170 165 160 DREDNPIAENLEPEWRDLRT AmpR DREDNPIAENLEPEWRDLRT t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a

PvuI 7620 155 150 145 140 VHDGMNHLFATLEKPGGITT AmpR VHDGMNHLFATLEKPGGITT a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g

7680 135 130 125 120 LLLNAATNDSMTIAASCLER AmpR LLLNAATNDSMTIAASCLER a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a

7740 115 110 105 100 VTMGDTLHKETVPSYEVLDN AmpR VTMGDTLHKETVPSYEVLDN a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g

7800 95 90 85 80 QSYHIRRGLQEQGADIRSLV AmpR A T A A T A C C Amp-R QSYHIRRGLQEQGADIRSLV t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g

7860 75 70 65 60 AGCLLVKFTSMMPFREEPRF AmpR G C G C C A C A T A G C Amp-R AGCLLVKFTSMMPFREEPRF g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t

7920 55 50 45 40 SELIKGSNLDLEIYGVRAGL AmpR SELIKGSNLDLEIYGVRAGL c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g

7980 35 30 25 QDEADKVKVLTEPH 20 signal sequence AFVPLC AmpR QDEADKVKVLTEPHAFVPLC t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t

8040 15 10 5 1 signal sequence FAAFFPILAVRFHQISM AmpR AmpR promoter FAAFFPILAVRFHQISM a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a

SspI 8100 AmpR promoter t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t

8160 AmpR promoter t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a

SgrDI 3ʹ 5ʹ 8166 g a c g t c c t g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.