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Sequence Analyzer: PM-GA Sequencing Result


Map View

AseI (4758) PciI (4693) pBR322ori-F (4538 .. 4557) ApaLI (4379) EcoO109I (3873) BsaI (3764) TK-pA-R (3629 .. 3648) PfoI (3550) BstBI (3457) Neo-F (3311 .. 3330) RsrII (3291) BsrDI (3008) PflFI - Tth111I (2893) FspI (2877) Neo-R (2701 .. 2720) EagI (2681) BspDI * - ClaI * (2615) StuI (2596) SfiI (2550) NdeI (189) SnaBI (295) CMV-F (474 .. 494) NheI (546) BmtI (550) mCherry-R (789 .. 807) AhdI (838) SbfI (999) mCherry-F (1057 .. 1076) BbsI (1079) Eco53kI (1138) SacI (1140) DsRed1-C (1251 .. 1274) AleI (1286) AfeI (1304) PflMI * (1324) HindIII (1355) SalI (1389) AccI (1390) Acc65I (1395) KpnI (1399) SacII (1402) PspOMI (1403) TspMI - XmaI (1406) ApaI (1407) SmaI (1408) BamHI (1410) XbaI * (1422) MfeI (1525) HpaI (1538) SV40pA-R (1576 .. 1595) BtsI - BtsαI (1614) EBV-rev (1630 .. 1649) MluI (1661) F1ori-F (1804 .. 1825) DraIII (1891) F1ori-R (2016 .. 2035) pBABE 3' (2278 .. 2298) PM-GA 4796 bp

Sequence View

5ʹ 3ʹ 60 CMV enhancer a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t

120 CMV enhancer c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g

180 CMV enhancer t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t

NdeI 240 CMV enhancer a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c

SnaBI 300 CMV enhancer g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a

360 CMV enhancer CMV promoter a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c

420 CMV promoter g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c

480 CMV promoter C G C A A A T CMV-F g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a

540 CMV promoter G G G C G G T A G G C G T G CMV-F *HVTL g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t

BmtINheI 600 MLCCMRRTKQVEK DALTGGGHQATHSSGFLNFF g a t c c g c t a g c g t g c c g c c a c c a t g c t g t g c t g t a t g a g a a g a a c c a a a c a g g t t g a a a a c t a g g c g a t c g c a c g g c g g t g g t a c g a c a c g a c a t a c t c t t c t t g g t t t g t c c a a c t t t t

660 NDDDQKIGGGGSGGGGSAMV IIVVLLNTTTTTTTTTTSHH a a a t g a t g a c g a c c a a a a g a t t g g t g g t g g t g g t a g t g g t g g t g g t g g t a g t g c t a t g g t t t t a c t a c t g c t g g t t t t c t a a c c a c c a c c a c c a t c a c c a c c a c c a c c a t c a c g a t a c c a

720 SKSEEVIKEFMRFKVRLEGS ALALLDDFLEHAELHAQLAG g a g c a a g a g c g a g g a g g t c a t c a a a g a g t t c a t g c g c t t c a a g g t g c g c t t g g a g g g c t c c t c g t t c t c g c t c c t c c a g t a g t t t c t c a a g t a c g c g a a g t t c c a c g c g a a c c t c c c g a g

780 MNGHEFEIEGEGEGRPYEGT HVAVLELDLALALAARVLAG c a t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g g c c g c c c t t a c g a g g g c a c g t a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g g a a t g c t c c c g t g

AhdI 840 G G T T C G A C T T C C A C T G G T T mCherry-R QTAKLKVTKGGPLPFAWDIL LGGLQLHGLAAGQGEGPVDQ c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g c g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c g c c g g g g g a c g g g a a g c g g a c c c t g t a g g a

900 SPLIMYGSKMYVKHPADVPD GGQDHVAGLHVHLVGGVDGI g t c c c c c c t g a t c a t g t a c g g c t c c a a g a t g t a c g t g a a g c a c c c c g c c g a c g t c c c c g a c a g g g g g g a c t a g t a c a t g c c g a g g t t c t a c a t g c a c t t c g t g g g g c g g c t g c a g g g g c t

960 YMKLSFPEGFKWERVMHFED VHLQGEGLAELPLAHHVELV t t a c a t g a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g g a g c g c g t g a t g c a c t t c g a g g a a a t g t a c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c c t c g c g c a c t a c g t g a a g c t c c t

SbfI 1020 GGLVTATQDSSLQDGTLIYK ATQHGRCLVGGQLVARQDVL c g g c g g t c t g g t g a c c g c g a c a c a g g a c t c c t c c c t g c a g g a c g g c a c g c t g a t c t a c a a g c c g c c a g a c c a c t g g c g c t g t g t c c t g a g g a g g g a c g t c c t g c c g t g c g a c t a g a t g t t

BbsI 1080 C C C C G T A A T G C A G A A G A A G A mCherry-F VKMRGTNFPPDGPVMQKKTL HLHAAGVEGGVAGYHLLLGQ g g t g a a g a t g c g c g g c a c c a a c t t c c c c c c c g a c g g c c c c g t a a t g c a g a a g a a g a c c t t c c a c t t c t a c g c g c c g t g g t t g a a g g g g g g g c t g c c g g g g c a t t a c g t c t t c t t c t g g a a

Eco53kISacI 1140 GWDYATERLYPEEGVLKGEL APIIGGLAQVGFFAHQLALE g g g c t g g g a t t a t g c c a c c g a g c g c c t g t a c c c c g a a g a a g g c g t g c t g a a g g g c g a g c t c c c g a c c c t a a t a c g g t g g c t c g c g g a c a t g g g g c t t c t t c c g c a c g a c t t c c c g c t c g a

1200 LGRLKLKDGGLNLVESKTIY QPAQLQLVAAEVQHLGLGDV c c t g g g g c g c c t g a a g c t g a a g g a c g g c g g c c t c a a c c t g g t g g a g t c c a a g a c c a t c t a g g a c c c c g c g g a c t t c g a c t t c c t g c c g c c g g a g t t g g a c c a c c t c a g g t t c t g g t a g a t

1260 A G C T G G A C A T DsRed1-C MAKKPVQLPGYYFVDTKLDI HGLLGHLQGAVVEHVGLQVD c a t g g c c a a g a a g c c c g t g c a a c t g c c c g g c t a c t a c t t c g t g g a c a c c a a g c t g g a c a t g t a c c g g t t c t t c g g g c a c g t t g a c g g g c c g a t g a t g a a g c a c c t g t g g t t c g a c c t g t a

AfeIAleI 1320 C A C C T C C C A C A A C G DsRed1-C TSHNEDYTIVEQYERSEGRH GGVVLVVGDHFLVLAGLAAV c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t a c g a g c g c t c c g a g g g c c g c c a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a t g c t c g c g a g g c t c c c g g c g g t

HindIIIPflMI* 1380 HLGHGTGST* VQPMAGAAGLLLKTFRQDAE c c a c c t g g g g c a t g g c a c c g g c a g c a c c t a a t a g a a g c t t g g t a a a c c g c t g a t c a g c c t g g t g g a c c c c g t a c c g t g g c c g t c g t g g a t t a t c t t c g a a c c a t t t g g c g a c t a g t c g g a

XbaI*SalIAccIAcc65IKpnISacIIPspOMITspMIXmaIApaISmaIBamHI 1440 VATSPVAPGPDVPDLYSIM c g a c t g c a g t c g a c g g t a c c g c g g g c c c g g g a t c c a c c g g a t c t a g a t a a c t g a t c a t a a g c t g a c g t c a g c t g c c a t g g c g c c c g g g c c c t a g g t g g c c t a g a t c t a t t g a c t a g t a t t

1500 t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g

HpaIMfeI 1560 SV40 poly(A) signal t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t

BtsαIBtsI 1620 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g

MluI 1680 SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a c g c g t a a a t t g t a a g c g t t t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t g c g c a t t t a a c a t t c g c a a

1740 f1 ori a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c

1800 f1 ori g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a

1860 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t

DraIII 1920 f1 ori a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c

1980 f1 ori g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a

2040 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g

2100 f1 ori g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a

2160 f1 ori AmpR promoter a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a

2220 AmpR promoter a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t

2280 AmpR promoter SV40 promoter SV40 enhancer C C T pBABE 3' t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t

2340 SV40 promoter SV40 enhancer T A C A C A C A G T C A A T C C C A pBABE 3' a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t

2400 SV40 promoter SV40 enhancer g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t

2460 SV40 promoter SV40 enhancer g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g

2520 SV40 promoter SV40 ori c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a

SfiI 2580 SV40 promoter SV40 ori t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c

BspDI*ClaI*StuI 2640 SV40 promoter SV40 ori g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c

EagI 2700 1 5 10 15 MIEQDGLHAGSPAAWVER NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWVER NeoR/KanR MIEQDGLHAGSPAAWVER t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c

2760 20 25 30 35 LFGYDWAQQTIGCSDAAVFR NeoR/KanR 20 25 30 35 LFGYDWAQQTIGCSDAAVFR NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R LFGYDWAQQTIGCSDAAVFR c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c

2820 40 45 50 55 LSAQGRPVLFVKTDLSGALN NeoR/KanR 40 45 50 55 LSAQGRPVLFVKTDLSGALN NeoR/KanR LSAQGRPVLFVKTDLSGALN c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a

FspI 2880 60 65 70 75 ELQDEAARLSWLATTGVPCA NeoR/KanR 60 65 70 75 ELQDEAARLSWLATTGVPCA NeoR/KanR ELQDEAARLSWLATTGVPCA g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t

Tth111IPflFI 2940 80 85 90 95 AVLDVVTEAGRDWLLLGEVP NeoR/KanR 80 85 90 95 AVLDVVTEAGRDWLLLGEVP NeoR/KanR AVLDVVTEAGRDWLLLGEVP g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c

3000 100 105 110 115 GQDLLSSHLAPAEKVSIMAD NeoR/KanR 100 105 110 115 GQDLLSSHLAPAEKVSIMAD NeoR/KanR GQDLLSSHLAPAEKVSIMAD *RVKSRGLFYGDHSI g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a

BsrDI 3060 120 125 130 135 AMRRLHTLDPATCPFDHQAK NeoR/KanR 120 125 130 135 AMRRLHTLDPATCPFDHQAK NeoR/KanR AMRRLHTLDPATCPFDHQAK CHPPQMRKIRSGAWEVVLRF g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t

3120 140 145 150 155 HRIERARTRMEAGLVDQDDL NeoR/KanR 140 145 150 155 HRIERARTRMEAGLVDQDDL NeoR/KanR HRIERARTRMEAGLVDQDDL MADLSCTSPHFGTKDILIIQ c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c

3180 160 165 170 175 DEEHQGLAPAELFARLKASM NeoR/KanR 160 165 170 175 DEEHQGLAPAELFARLKASM NeoR/KanR DEEHQGLAPAELFARLKASM VFLMLPERWGFQEGPELRAH g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c

3240 180 185 190 195 PDGEDLVVTHGDACLPNIMV NeoR/KanR 180 185 190 195 PDGEDLVVTHGDACLPNIMV NeoR/KanR PDGEDLVVTHGDACLPNIMV GVALIEDHGMAIGAQRIDHH c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c

RsrII 3300 200 205 210 215 ENGRFSGFIDCGRLGVADRY NeoR/KanR 200 205 210 215 ENGRFSGFIDCGRLGVADRY NeoR/KanR ENGRFSGFIDCGRLGVADRY FIAAKRSEDVTAPQTHRVAI g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a

3360 220 225 230 235 QDIALATRDIAEELGGEWAD NeoR/KanR 220 225 230 235 QDIALATRDIAEELGGEWAD NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F QDIALATRDIAEELGGEWAD LVYRQSGTINSFLKAAFPSV c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g

3420 240 245 250 255 RFLVLYGIAAPDSQRIAFYR NeoR/KanR 240 245 250 255 RFLVLYGIAAPDSQRIAFYR NeoR/KanR RFLVLYGIAAPDSQRIAFYR AEEHKVTDGSGIRLADGEIA c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g

BstBI 3480 260 265 LLDEFF* NeoR/KanR 260 265 LLDEFF* NeoR/KanR LLDEFF* MTDQAT KKVLEESRSEPTRFSRGLSA c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g

3540 PNLPSRDFDSTAAFYERLGF WGAM c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c

PfoI 3600 GIVFRDAGWMILQRGDLMLE g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a

3660 A C T T T G T G C C T T C C T C T G T T TK-pA-R FFAHPRGRLTETRKETIPEG t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t

3720 HSV TK poly(A) signal TRAMTAIKRQNKTHGVGSFV c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g

BsaI 3780 HKRGVRSQGWHSVDTPPRPH a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a

3840 WGQYARVSSFSPPHPPSSGE g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c

EcoO109I 3900 GPGLAANVGAAGPAIASGYS g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a

3960 YIL* a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g

4020 t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c

4080 ori a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a

4140 ori g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g

4200 ori c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g

4260 ori t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c

4320 ori c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a

ApaLI 4380 ori t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a

4440 ori g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g

4500 ori t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t

4560 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t

4620 ori g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c

4680 ori g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a

PciI 4740 g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t

AseI 3ʹ 5ʹ 4796 c c g c c a t g c a t t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c g g c g g t a c g t a a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.