Addgene Fus-BcLOV-mCh Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: Fus-BcLOV-mCh Sequencing Result


Map View

SV40pro-F (8388 .. 8407) pBABE 3' (8141 .. 8161) F1ori-F (8001 .. 8022) F1ori-R (7791 .. 7810) BGH-rev (7428 .. 7445) ApaI (7407) PspOMI (7403) XbaI * (7397) BsrGI (7383) SgrAI (7364) BbvCI (7221) PflMI (7122) mCherry-F (7096 .. 7115) mCherry-R (6828 .. 6846) EcoRI (6653) BlpI (6245) BmgBI (5974) Bsu36I (4818) PasI (4579) SrfI (4426) BamHI (4358) HindIII (4340) AflII (4337) BmtI (4328) NheI (4324) T7 (4292 .. 4311) AvrII (52) EagI (168) Neo-R (188 .. 207) PflFI - Tth111I (380) Neo-F (798 .. 817) BstBI (944) SV40pA-R (1139 .. 1158) BsmI (1182) EBV-rev (1193 .. 1212) BstZ17I (1234) M13 Reverse (1272 .. 1288) lac operator M13/pUC Reverse (1285 .. 1307) CAP binding site L4440 (1503 .. 1520) PciI (1613) pBR322ori-F (1754 .. 1773) PvuI (2876) Amp-R (3056 .. 3075) SspI (3310) SgrDI (3427) BglII (3441) pRS-marker (3473 .. 3492) MfeI (3590) MluI (3657) SpeI (3678) CMV enhancer NdeI (3913) SnaBI (4019) CMV-F (4198 .. 4218) Fus-BcLOV-mCh 8408 bp

Sequence View

AvrII 5ʹ 3ʹ 60 SV40 promoter SV40 ori c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a

120 SV40 promoter t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a c a g g a t g a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t g t c c t a c

EagI 180 1 5 10 15 MIEQDGLHAGSPAAWV NeoR/KanR MIEQDGLHAGSPAAWV a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a

240 20 25 30 35 ERLFGYDWAQQTIGCSDAAV NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R ERLFGYDWAQQTIGCSDAAV g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a

300 40 45 50 55 FRLSAQGRPVLFVKTDLSGA NeoR/KanR FRLSAQGRPVLFVKTDLSGA g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g

360 60 65 70 75 LNELQDEAARLSWLATTGVP NeoR/KanR LNELQDEAARLSWLATTGVP c c t g a a t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c g g a c t t a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g

Tth111IPflFI 420 80 85 90 95 CAAVLDVVTEAGRDWLLLGE NeoR/KanR CAAVLDVVTEAGRDWLLLGE t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t

480 100 105 110 115 VPGQDLLSSHLAPAEKVSIM NeoR/KanR VPGQDLLSSHLAPAEKVSIM *RVKSRGLFYGDH a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a

540 120 125 130 135 ADAMRRLHTLDPATCPFDHQ NeoR/KanR ADAMRRLHTLDPATCPFDHQ SICHPPQMRKIRSGAWEVVL g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t

600 140 145 150 155 AKHRIERARTRMEAGLVDQD NeoR/KanR AKHRIERARTRMEAGLVDQD RFMADLSCTSPHFGTKDILI a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t

660 160 165 170 175 DLDEEHQGLAPAELFARLKA NeoR/KanR DLDEEHQGLAPAELFARLKA IQVFLMLPERWGFQEGPELR t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g

720 180 185 190 195 RMPDGEDLVVTHGDACLPNI NeoR/KanR RMPDGEDLVVTHGDACLPNI AHGVALIEDHGMAIGAQRID g c g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c g c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a

780 200 205 210 215 MVENGRFSGFIDCGRLGVAD NeoR/KanR MVENGRFSGFIDCGRLGVAD HHFIAAKRSEDVTAPQTHRV c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t

840 220 225 230 235 RYQDIALATRDIAEELGGEW NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F RYQDIALATRDIAEELGGEW AILVYRQSGTINSFLKAAFP c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c

900 240 245 250 255 ADRFLVLYGIAAPDSQRIAF NeoR/KanR ADRFLVLYGIAAPDSQRIAF SVAEEHKVTDGSGIRLADGE g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a

BstBI 960 260 265 YRLLDEFF* NeoR/KanR YRLLDEFF* IAKKVLEESRSEPTRFSRGL c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t

1020 SAWGAM g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c

1080 g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c

1140 SV40 poly(A) signal C G SV40pA-R c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g

BsmI 1200 SV40 poly(A) signal G T G G T T T G EBV-rev T T A T C G T A G T G T T T A A A G SV40pA-R a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c

BstZ17I 1260 SV40 poly(A) signal T C C A A A C T C A T C EBV-rev t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c

1320 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g

1380 -10 -35 lac promoter CAP binding site a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g

1440 CAP binding site a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c

1500 c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a

1560 G A G C G A G T G A C T G A G C G A L4440 t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g

PciI 1620 t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t

1680 ori g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a

1740 ori a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g

1800 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a

1860 ori g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c

1920 ori c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c

1980 ori g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a

2040 ori c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c

2100 ori a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g

2160 ori g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t

2220 ori a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a

2280 ori g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a

2340 t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t

2400 t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g

2460 285 280 *WHKILSAG AmpR *WHKILSAG t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a

2520 275 270 265 260 IEAIQRNREDMTAQSGTTYI AmpR IEAIQRNREDMTAQSGTTYI a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t

2580 255 250 245 240 VVIRSPKGDPGLAAIIGRSG AmpR VVIRSPKGDPGLAAIIGRSG a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t

2640 235 230 225 220 REGAGSKDAIFWGAPLASRL AmpR REGAGSKDAIFWGAPLASRL c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t

2700 215 210 205 200 LPGAVKDAEMWDILQQRSAL AmpR LPGAVKDAEMWDILQQRSAL a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t

2760 195 190 185 180 TLLEGTLLKRLTTAMAVPMT AmpR TLLEGTLLKRLTTAMAVPMT g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c

2820 175 170 165 160 TDREDNPIAENLEPEWRDLR AmpR TDREDNPIAENLEPEWRDLR g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t

PvuI 2880 155 150 145 140 TVHDGMNHLFATLEKPGGIT AmpR TVHDGMNHLFATLEKPGGIT g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a

2940 135 130 125 120 TLLLNAATNDSMTIAASCLE AmpR TLLLNAATNDSMTIAASCLE g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a

3000 115 110 105 100 RVTMGDTLHKETVPSYEVLD AmpR RVTMGDTLHKETVPSYEVLD c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t

3060 95 90 85 80 NQSYHIRRGLQEQGADIRSL AmpR A T A A T Amp-R NQSYHIRRGLQEQGADIRSL t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a

3120 75 70 65 60 VAGCLLVKFTSMMPFREEPR AmpR A C C G C G C C A C A T A G C Amp-R VAGCLLVKFTSMMPFREEPR a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t

3180 55 50 45 40 FSELIKGSNLDLEIYGVRAG AmpR FSELIKGSNLDLEIYGVRAG a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g

3240 35 30 25 LQDEADKVKVLTEPH 20 signal sequence AFVPL AmpR LQDEADKVKVLTEPHAFVPL a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c

3300 15 10 5 1 signal sequence CFAAFFPILAVHFHQISM AmpR AmpR promoter CFAAFFPILAVHFHQISM c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a t g g a a a t g t t g a a t a c t c a t a c t c t t c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t a c c t t t a c a a c t t a t g a g t a t g a g a a g

SspI 3360 AmpR promoter c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a

3420 AmpR promoter g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t

BglIISgrDI 3480 A T G T T A G A pRS-marker c c t g a c g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g g a c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a

3540 C G A G A C T A C G G C pRS-marker g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g c g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c

MfeI 3600 a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t

MluI 3660 a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g

SpeI 3720 CMV enhancer g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t

3780 CMV enhancer g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g

3840 CMV enhancer c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c

3900 CMV enhancer g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g

NdeI 3960 CMV enhancer a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c

SnaBI 4020 CMV enhancer c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c

4080 CMV enhancer CMV promoter t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a

4140 CMV promoter a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a

4200 CMV promoter C G C CMV-F t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g

4260 CMV promoter A A A T G G G C G G T A G G C G T G CMV-F a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t

4320 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 g a g a a c c c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t c t t g g g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c

BamHIAflIIHindIIIBmtINheI 4380 MASNDY c t g g c t a g c g t t t a a a c t t a a g c t t g g t a c c g a g c t c g g a t c c a t g g c c t c a a a c g a t t a g a c c g a t c g c a a a t t t g a a t t c g a a c c a t g g c t c g a g c c t a g g t a c c g g a g t t t g c t a a t

SrfI 4440 TQQATQSYGAYPTQPGQGYS MGPTPPSPGRAI t a c c c a a c a a g c a a c c c a a a g c t a t g g g g c c t a c c c c a c c c a g c c c g g g c a g g g c t a t t c a t g g g t t g t t c g t t g g g t t t c g a t a c c c c g g a t g g g g t g g g t c g g g c c c g t c c c g a t a a g

4500 QQSSQPYGQQSYSGYSQSTD PSRAVSPTDSRVTVVIASPR c c a g c a g a g c a g t c a g c c c t a c g g a c a g c a g a g t t a c a g t g g t t a t a g c c a g t c c a c g g a g g t c g t c t c g t c a g t c g g g a t g c c t g t c g t c t c a a t g t c a c c a a t a t c g g t c a g g t g c c t

4560 TSGYGQSSYSSYGQSQNTGY TLQAMARAAILLMARARTQA c a c t t c a g g c t a t g g c c a g a g c a g c t a t t c t t c t t a t g g c c a g a g c c a g a a c a c a g g c t a g t g a a g t c c g a t a c c g g t c t c g t c g a t a a g a a g a a t a c c g g t c t c g g t c t t g t g t c c g a t

PasI 4620 GTQSTPQGYGSTGGYGSSQS MELSQLPRDMARLAAMAVAR t g g a a c t c a g t c a a c t c c c c a g g g a t a t g g c t c g a c t g g c g g c t a t g g c a g t a g c c a g a g a c c t t g a g t c a g t t g a g g g g t c c c t a t a c c g a g c t g a c c g c c g a t a c c g t c a t c g g t c t c

4680 SQSSYGQQSSYPGYGQQPAP APNRLTGSSPPILAMASSQL c t c c c a a t c g t c t t a c g g g c a g c a g t c c t c c t a t c c t g g c t a t g g c c a g c a g c c a g c t c c g a g g g t t a g c a g a a t g c c c g t c g t c a g g a g g a t a g g a c c g a t a c c g g t c g t c g g t c g a g g

4740 SSTSGSYGSSSQSSSYGQPQ PAAPREVTVAVLRAAAMGSP c a g c a g c a c c t c g g g a a g t t a c g g t a g c a g t t c t c a g a g c a g c a g c t a t g g g c a g c c c c a g t c g t c g t g g a g c c c t t c a a t g c c a t c g t c a a g a g t c t c g t c g t c g a t a c c c g t c g g g g t

4800 1 WELQ PAA* WELQut site SGSYSQQPSYGGQQQSYGQQ RVGATASSLAMVDSSKAMDS g a g t g g g a g c t a c a g c c a g c a g c c t a g c t a t g g t g g a c a g c a g c a a a g c t a t g g a c a g c a c t c a c c c t c g a t g t c g g t c g t c g g a t c g a t a c c a c c t g t c g t c g t t t c g a t a c c t g t c g t

Bsu36I 4860 QSYNPPQGYGQQNQYNSGSG SKAIIPLRAMDSRTSTTAVA g c a a a g c t a t a a t c c c c c t c a g g g c t a t g g a c a g c a g a a c c a g t a c a a c a g c g g t a g c g g c g t t t c g a t a t t a g g g g g a g t c c c g a t a c c t g t c g t c t t g g t c a t g t t g t c g c c a t c g c c

4920 SGSMATDAIDHYYQNRDGPP AAVAWPQTQSTITTRTETAP c a g c g g t a g c a t g g c c a c a g a c g c a a t c g a c c a t t a c t a c c a g a a c a g a g a c g g c c c c c c g t c g c c a t c g t a c c g g t g t c t g c g t t a g c t g g t a a t g a t g g t c t t g t c t c t g c c g g g g g g

4980 SRSFSDEYDSYNKTAPLPPL LPGALATNTTHTIRQPLCHL t t c c a g g a g c t t t a g c g a c g a a t a c g a c t c a t a c a a t a a g a c a g c c c c t c t g c c a c c t c t a a g g t c c t c g a a a t c g c t g c t t a t g c t g a g t a t g t t a t t c t g t c g g g g a g a c g g t g g a g a

5040 SACPERAQTPNSKRIPITPN* g a g c g c c t g c c c a g a g c g c g c c c a g a c c c c t a a c a g c a a g a g a a t c c c c a t c a c a c c c a a c t c g c g g a c g g g t c t c g c g c g g g t c t g g g g a t t g t c g t t c t c t t a g g g g t a g t g t g g g t t

5100 SAKSTSESPRRITFSRDAGS t t c c g c c a a g a g c a c c t c c g a g t c t c c t c g g a g a a t c a c a t t c t c t c g c g a c g c a g g c a g a a g g c g g t t c t c g t g g a g g c t c a g a g g a g c c t c t t a g t g t a a g a g a g c g c t g c g t c c g t c

5160 STKGSTQTSPSNQGSVSSRN c t c c a c c a a g g g c a g c a c c c a g a c a a g c c c a t c c a a c c a g g g c t c c g t g t c t a g c c g g a a g a g g t g g t t c c c g t c g t g g g t c t g t t c g g g t a g g t t g g t c c c g a g g c a c a g a t c g g c c t t

5220 GSNGNGLGLSSAGNFADFFS t g g c t c t a a c g g a a a t g g c c t g g g c c t g t c c t c t g c c g g c a a c t t c g c c g a t t t c t t t a g a c c g a g a t t g c c t t t a c c g g a c c c g g a c a g g a g a c g g c c g t t g a a g c g g c t a a a g a a a t c

5280 SEVFYIVLHNPTTAHRFLKF c t c c g a g g t g t t t t a t a t c g t g c t g c a c a a t c c c a c c a c a g c c c a c a g a t t c c t g a a g t t g a g g c t c c a c a a a a t a t a g c a c g a c g t g t t a g g g t g g t g t c g g g t g t c t a a g g a c t t c a a

5340 CQSRACSENLEFLQKIDEYN t t g c c a g a g c a g g g c c t g t t c c g a g a a c c t g g a g t t t c t g c a g a a g a t c g a c g a g t a c a a a a c g g t c t c g t c c c g g a c a a g g c t c t t g g a c c t c a a a g a c g t c t t c t a g c t g c t c a t g t t

5400 RLIDEATRLLSTIYSTYVSS t a g g c t g a t c g a t g a g g c c a c a c g c c t g c t g t c t a c c a t c t a c a g c a c a t a c g t g a g c a g a t c c g a c t a g c t a c t c c g g t g t g c g g a c g a c a g a t g g t a g a t g t c g t g t a t g c a c t c g t c

5460 EAPKQINISNSHARRLSHDI c g a g g c c c c t a a g c a g a t c a a c a t c t c t a a t a g c c a c g c c a g g c g c c t g a g c c a c g a c a t g c t c c g g g g a t t c g t c t a g t t g t a g a g a t t a t c g g t g c g g t c c g c g g a c t c g g t g c t g t a

5520 KGATHNVLPGLEDIFNGSQE c a a g g g a g c a a c c c a c a a c g t g c t g c c c g g c c t g g a g g a t a t c t t c a a t g g c t c c c a g g a g t t c c c t c g t t g g g t g t t g c a c g a c g g g c c g g a c c t c c t a t a g a a g t t a c c g a g g g t c c t

5580 QIEKLLASDLYPRFVKHQVT g c a g a t c g a g a a g c t g c t g g c c t c t g a c c t g t a t c c t a g a t t t g t g a a g c a c c a g g t g a c c g t c t a g c t c t t c g a c g a c c g g a g a c t g g a c a t a g g a t c t a a a c a c t t c g t g g t c c a c t g

5640 ASATMALANDKERYPGLGDC c g c a t c c g c c a c a a t g g c c c t g g c a a a c g a c a a g g a g a g g t a c c c a g g c c t g g g c g a t t g g c g t a g g c g g t g t t a c c g g g a c c g t t t g c t g t t c c t c t c c a t g g g t c c g g a c c c g c t a a c

5700 FCLTDPRRADNPIVFASDGF c t t c t g t c t g a c a g a c c c a c g g a g a g c c g a t a a t c c c a t c g t g t t c g c a a g c g a c g g a t t g a a g a c a g a c t g t c t g g g t g c c t c t c g g c t a t t a g g g t a g c a c a a g c g t t c g c t g c c t a a

5760 VSVTGYSRSDIIPRNCRFLQ c g t g a g c g t g a c c g g c t a t t c c c g c t c t g a t a t c a t c c c a a g g a a c t g c c g c t t c c t g c a g c a c t c g c a c t g g c c g a t a a g g g c g a g a c t a t a g t a g g g t t c c t t g a c g g c g a a g g a c g t

5820 GSFTDRQATKRLRTSIENRE g g g c a g c t t t a c c g a c c g g c a g g c c a c a a a g a g g c t g c g c a c c t c c a t c g a g a a t c g c g a c c c g t c g a a a t g g c t g g c c g t c c g g t g t t t c t c c g a c g c g t g g a g g t a g c t c t t a g c g c t

5880 ETVELLLNYRKNGDPFWNLL g g a g a c a g t g g a g c t g c t g c t g a a c t a t c g g a a g a a t g g c g a t c c c t t t t g g a a c c t g c t c c t c t g t c a c c t c g a c g a c g a c t t g a t a g c c t t c t t a c c g c t a g g g a a a a c c t t g g a c g a

5940 YVSPLLDGNGDVRFFLGGQI g t a c g t g t c c c c t c t g c t g g a c g g c a a t g g c g a t g t g a g a t t c t t t c t g g g c g g c c a g a t c a t g c a c a g g g g a g a c g a c c t g c c g t t a c c g c t a c a c t c t a a g a a a g a c c c g c c g g t c t a

BmgBI 6000 NCSTTIHSRTDVLRILSLND c a a c t g t t c t a c c a c a a t c c a c a g c c g g a c c g a c g t g c t g a g a a t c c t g a g c c t g a a c g a g t t g a c a a g a t g g t g t t a g g t g t c g g c c t g g c t g c a c g a c t c t t a g g a c t c g g a c t t g c t

6060 EDTGSFIEGSNKTPSVRSKE c g a g g a t a c a g g c t c c t t c a t c g a g g g c t c t a a t a a g a c c c c c a g c g t g c g g t c c a a g g a g c t c c t a t g t c c g a g g a a g t a g c t c c c g a g a t t a t t c t g g g g g t c g c a c g c c a g g t t c c t

6120 SNNASNYGKTSFFKSFKKYK g t c t a a c a a t g c c t c c a a c t a t g g c a a g a c a t c c t t c t t t a a g t c t t t t a a g a a g t a c a a c a g a t t g t t a c g g a g g t t g a t a c c g t t c t g t a g g a a g a a a t t c a g a a a a t t c t t c a t g t t

6180 ANTVEIRDEAGMEGELIDRI g g c c a a t a c c g t g g a g a t c a g a g a c g a g g c c g g c a t g g a g g g c g a g c t g a t c g a t a g g a t c c g g t t a t g g c a c c t c t a g t c t c t g c t c c g g c c g t a c c t c c c g c t c g a c t a g c t a t c c t a

6240 GKLQFKTQVEEFYTAYSKYL c g g c a a g c t g c a g t t c a a g a c a c a g g t g g a g g a g t t t t a c a c c g c c t a t t c t a a g t a c c t g c c g t t c g a c g t c a a g t t c t g t g t c c a c c t c c t c a a a a t g t g g c g g a t a a g a t t c a t g g a

BlpI 6300 VLSYQPQSQNLIVKYFSPGI g g t g c t g a g c t a t c a g c c a c a g t c c c a g a a c c t g a t c g t g a a g t a c t t c a g c c c c g g c a t c c a c g a c t c g a t a g t c g g t g t c a g g g t c t t g g a c t a g c a c t t c a t g a a g t c g g g g c c g t a

6360 VDMLNLNMPNGLVAPIVDKD c g t g g a t a t g c t g a a c c t g a a t a t g c c t a a t g g c c t g g t g g c c c c a a t c g t g g a c a a g g a g c a c c t a t a c g a c t t g g a c t t a t a c g g a t t a c c g g a c c a c c g g g g t t a g c a c c t g t t c c t

6420 IFKVLTEHSPSSVPRTFKNV t a t c t t c a a g g t g c t g a c a g a g c a c t c c c c t t c c t c t g t g c c a c g g a c c t t t a a g a a c g t a t a g a a g t t c c a c g a c t g t c t c g t g a g g g g a a g g a g a c a c g g t g c c t g g a a a t t c t t g c a

6480 VREGIKAGRAVSVETGLLTG g g t g a g g g a a g g c a t c a a a g c a g g c a g g g c c g t g t c t g t g g a g a c a g g c c t g c t g a c a g g c c a c t c c c t t c c g t a g t t t c g t c c g t c c c g g c a c a g a c a c c t c t g t c c g g a c g a c t g t c c

6540 IEEIKKSQNIFSGTTANRER c a t c g a g g a g a t c a a g a a g t c t c a g a a c a t c t t c a g c g g c a c c a c a g c c a a t c g g g a g a g g t a g c t c c t c t a g t t c t t c a g a g t c t t g t a g a a g t c g c c g t g g t g t c g g t t a g c c c t c t c

6600 GWKKAEEKYVCHFTPCKDEM a g g c t g g a a g a a g g c c g a g g a g a a g t a c g t g t g c c a c t t t a c c c c c t g t a a g g a t g a g a t t c c g a c c t t c t t c c g g c t c c t c t t c a t g c a c a c g g t g a a a t g g g g g a c a t t c c t a c t c t a

EcoRI 6660 GRVGWVVLTVAPKLERFEFG g g g c a g g g t c g g c t g g g t g g t g c t g a c t g t c g c t c c t a a a c t g g a a a g a t t t g a a t t c g g c c c g t c c c a g c c g a c c c a c c a c g a c t g a c a g c g a g g a t t t g a c c t t t c t a a a c t t a a g c c

6720 1 5 10 MVSKGEEDNMAII mCherry GGSGGGSMVSKGEEDNMAII t g g t g g a t c a g g t g g a g g a t c t a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c c a t c a t a c c a c c t a g t c c a c c t c c t a g a t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g g t a g t a

6780 15 20 25 30 KEFMRFKVHMEGSVNGHEFE mCherry KEFMRFKVHMEGSVNGHEFE c a a g g a g t t c a t g c g c t t c a a g g t g c a c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g t t c c t c a a g t a c g c g a a g t t c c a c g t g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t

6840 35 40 45 50 IEGEGEGRPYEGTQTAKLKV mCherry G G T T C G A C T T C C A mCherry-R IEGEGEGRPYEGTQTAKLKV g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a

6900 55 60 65 70 TKGGPLPFAWDILSPQFMYG mCherry C T G G T T mCherry-R TKGGPLPFAWDILSPQFMYG g a c c a a g g g t g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t g g t t c c c a c c g g g g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c

6960 75 80 85 90 SKAYVKHPADIPDYLKLSFP mCherry SKAYVKHPADIPDYLKLSFP c t c c a a g g c c t a c g t g a a g c a c c c c g c c g a c a t c c c c g a c t a c t t g a a g c t g t c c t t c c c g a g g t t c c g g a t g c a c t t c g t g g g g c g g c t g t a g g g g c t g a t g a a c t t c g a c a g g a a g g g

7020 95 100 105 110 EGFKWERVMNFEDGGVVTVT mCherry EGFKWERVMNFEDGGVVTVT c g a g g g c t t c a a g t g g g a g c g c g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g a c g c t c c c g a a g t t c a c c c t c g c g c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c t g

7080 115 120 125 130 QDSSLQDGEFIYKVKLRGTN mCherry QDSSLQDGEFIYKVKLRGTN c c a g g a c t c c t c c c t g c a g g a c g g c g a g t t c a t c t a c a a g g t g a a g c t g c g c g g c a c c a a g g t c c t g a g g a g g g a c g t c c t g c c g c t c a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g t t

PflMI 7140 135 140 145 150 FPSDGPVMQKKTMGWEASSE mCherry C C C C G T A A T G C A G A A G A A G A mCherry-F FPSDGPVMQKKTMGWEASSE c t t c c c c t c c g a c g g c c c c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c t c c g a g a a g g g g a g g c t g c c g g g g c a t t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g a g g c t

7200 155 160 165 170 RMYPEDGALKGEIKQRLKLK mCherry RMYPEDGALKGEIKQRLKLK g c g g a t g t a c c c c g a g g a c g g c g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g a a c g c c t a c a t g g g g c t c c t g c c g c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c t t

BbvCI 7260 175 180 185 190 DGGHYDAEVKTTYKAKKPVQ mCherry DGGHYDAEVKTTYKAKKPVQ g g a c g g c g g c c a c t a c g a c g c t g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a c c t g c c g c c g g t g a t g c t g c g a c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t

7320 195 200 205 210 LPGAYNVNIKLDITSHNEDY mCherry LPGAYNVNIKLDITSHNEDY g c t g c c c g g c g c c t a c a a c g t c a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c g a c g g g c c g c g g a t g t t g c a g t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t

SgrAI 7380 215 220 225 230 TIVEQYERAEGRHSTGGMDE mCherry TIVEQYERAEGRHSTGGMDE c a c c a t c g t g g a a c a g t a c g a a c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g t g g t a g c a c c t t g t c a t g c t t g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t

XbaI*PspOMIApaIBsrGI 7440 235 LYK* mCherry bGH poly(A) signal G G A G C T G A C A C G G BGH-rev LYK* g c t g t a c a a g t a a t g a t c t a g a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c c g a c a t g t t c a t t a c t a g a t c t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g

7500 bGH poly(A) signal A A G A T BGH-rev t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c

7560 bGH poly(A) signal t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c

7620 bGH poly(A) signal g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t

7680 bGH poly(A) signal c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c

7740 f1 ori c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a

7800 f1 ori G A G G A A A G C G F1ori-R g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g

7860 f1 ori A A A G A A G G G A F1ori-R t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c

7920 f1 ori g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t

7980 f1 ori g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a

8040 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a

8100 f1 ori c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t

8160 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C pBABE 3' t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c

8220 SV40 promoter A pBABE 3' t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c

8280 SV40 promoter t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c

8340 SV40 promoter SV40 ori c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a

8400 SV40 promoter SV40 ori T A T T T A T G C A G A G SV40pro-F c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c

3ʹ 5ʹ 8408 SV40 promoter SV40 ori G C C G A G G SV40pro-F g c c g a g g c c g g c t c c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.