Addgene pLVTHM-mBrn2-3xflag Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pLVTHM-mBrn2-3xflag Sequencing Result


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EBV-rev (11,491 .. 11,510) SV40pA-R (11,437 .. 11,456) SV40 NLS pBABE 3' (9292 .. 9312) L4440 (9205 .. 9222) pBR322ori-F (8952 .. 8971) AhdI (8219) FspI (7996) PvuI (7850) Amp-R (7650 .. 7669) AmpR promoter pBRforEco (7241 .. 7259) pGEX 3' (7181 .. 7203) pRS-marker (7062 .. 7081) pBRrevBam (6829 .. 6848) SgrAI (6801) BmtI (6763) NheI (6759) BsrGI (6598) SP6 (6522 .. 6539) SP6 promoter XbaI (6500) loxP H1 (6146 .. 6165) BstBI (6009) EcoRI (6005) PmlI (291) BspEI (309) EagI - NotI (1145) AleI (1574) KflI - PpuMI (1931) SalI (2052) FseI (2851) PasI (2895) EF1a-F (3273 .. 3293) SwaI (3340) PacI (3349) AbsI (3509) PmeI (3518) Bsu36I (4171) BmgBI (4398) MluI (4858) 3xFLAG SpeI (4942) NdeI (4949) WPRE-R (5009 .. 5029) SnaBI (5552) MscI * (5558) Acc65I (5562) KpnI (5566) BamHI (5690) pLVTHM-mBrn2-3xflag 11,700 bp

Sequence View

5ʹ 3ʹ 60 3' LTR t t g g a a g g g c t a a t t c a c t c c c a a a g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a a c c t t c c c g a t t a a g t g a g g g t t t c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g

120 3' LTR a c a c a a g g c t a c t t c c c t g a t t a g c a g a a c t a c a c a c c a g g g c c a g g g g t c a g a t a t c c a t g t g t t c c g a t g a a g g g a c t a a t c g t c t t g a t g t g t g g t c c c g g t c c c c a g t c t a t a g g t

180 3' LTR c t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c c a g a t a a g g t a g a a g a g g c c g a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g g t c t a t t c c a t c t t c t c c g g

240 3' LTR a a t a a a g g a g a g a a c a c c a g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g t t a t t t c c t c t c t t g t g g t c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c

PmlI 300 3' LTR g a g a g a g a a g t g t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c g t g g c c c g a c t c t c t c t t c a c a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g c a c c g g g c t

BspEI 360 3' LTR g a g c t g c a t c c g g a g t a c t t c a a g a a c t g c t g a t a t c g a g c t t g c t a c a a g g g a c t t t c c c t c g a c g t a g g c c t c a t g a a g t t c t t g a c g a c t a t a g c t c g a a c g a t g t t c c c t g a a a g g

420 3' LTR g c t g g g g a c t t t c c a g g g a g g c g t g g c c t g g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a c g a c c c c t g a a a g g t c c c t c c g c a c c g g a c c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t

480 3' LTR t c c t g c a t a t a a g c a g c t g c t t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g g a c g t a t a t t c g t c g a c g a a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c

540 3' LTR a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g

600 3' LTR t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a

660 3' LTR c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t

720 HIV-1 Ψ g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c

780 HIV-1 Ψ g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c

840 HIV-1 Ψ a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c

900 g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c

960 g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g

1020 t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t

1080 t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g

1140 a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c

EagINotI 1200 MRDNWRSE c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t

1260 LYKYKVVKIEPLGVAPTKAK a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t

1320 RRE RRVVQREKRAVGIGALFLGF g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a

1380 RRE LGAAGSTMGAASMTLTVQAR c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c

1440 RRE QLLSGIVQQQNNLLRAIEAQ a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t

1500 RRE QHLLQLTVWGIKQLQARILA a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g

1560 RRE VERYLKDQQLLGIWGCSGKL t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a

AleI 1620 ICTTAVPWNASWSNKSLEQI c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a

1680 WNHTTWMEWDREINNYTSLI t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a

1740 1 5 KNEQELLE gp41 peptide HSLIEESQNQQEKNEQELLE a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t

1800 10 15 LDKWASL (in frame with gp41 peptide) WNWFNITNWLWYI gp41 peptide LDKWASLWNWFNITNWLWYI a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a

1860 (in frame with gp41 peptide) KLFIMIVGGLVGLRIVFAVL KLFIMIVGGLVGLRIVFAVL a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a

1920 (in frame with gp41 peptide) SIVNRVRQGYSPLSFQTHLP SIVNRVRQGYSPLSFQTHLP t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g

PpuMIKflI 1980 (in frame with gp41 peptide) TPRGPDRPEGIEEEGGERDR TPRGPDRPEGIEEEGGERDR a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c

2040 (in frame with gp41 peptide) DRSIRLVNGSRRYRSRD* DRSIRLVNGSRRYRSRD* a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g a t c a c g a g a c t a g c c t c g t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c t a g t g c t c t g a t c g g a g c

SalI 2100 a c c a t c g c g a t g t c g a c g a t a a g c t t t g c a a a g a t g g a t a a a g t t t t a a a c a g a g a g g a a t g g t a g c g c t a c a g c t g c t a t t c g a a a c g t t t c t a c c t a t t t c a a a a t t t g t c t c t c c t t

2160 EF-1 α promoter t c t t t g c a g c t a a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t g g c t c c g g t g c c c g a g a a a c g t c g a t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a c c g a g g c c a c g g g c

2220 EF-1 α promoter t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t

2280 EF-1 α promoter t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c

2340 EF-1 α promoter g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t

2400 EF-1 α promoter EF-1 α intron A c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c

2460 EF-1 α promoter EF-1 α intron A *KWRA c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c t g g c t g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g a c c g a

2520 EF-1 α promoter EF-1 α intron A ATRSEQDRAEPQFHTPSNSA g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a g g c c c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c t c c g g

2580 EF-1 α promoter EF-1 α intron A KRKLLGKAEHKLQPRAQASP t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t g g g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g a c c c c

2640 EF-1 α promoter EF-1 α intron A GGRAFRTAGERRDRQKRYTE c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a

2700 EF-1 α promoter EF-1 α intron A LWKFNKIVQQSAKKRALYDQ a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a

2760 EF-1 α promoter EF-1 α intron A LHPGLDACQYKPKQPRPRRR a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c

2820 EF-1 α promoter EF-1 α intron A PGHTGACMNPSAPGALAAVS g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c

FseI 2880 EF-1 α promoter EF-1 α intron A FRVPTTELQGAQEPAQGRAA a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g

PasI 2940 EF-1 α promoter EF-1 α intron A TYRGARPPLAPGTPVLQTLP g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t

3000 EF-1 α promoter EF-1 α intron A FIAAERGQQLSSLISSAASP a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c t c g g g t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g a g c c c

3060 EF-1 α promoter EF-1 α intron A LAPPHTVWVFSFPRETRLRR a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c g c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g c a g c g

3120 EF-1 α promoter EF-1 α intron A KM t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g a g c t t a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c t c g a a

3180 EF-1 α promoter EF-1 α intron A t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g

3240 EF-1 α promoter EF-1 α intron A t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a

3300 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a

PacISwaI 3360 EF-1 α promoter EF-1 α intron A t t t t c t t c c a t t t c a g g t g t c g t g a g g a a t t t c g a c a t t t a a a t t t a a t t a a t c t c g a c g a a a a g a a g g t a a a g t c c a c a g c a c t c c t t a a a g c t g t a a a t t t a a a t t a a t t a g a g c t g c

3420 cPPT/CTS g t a t c g g t t a a c t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t c a t a g c c a a t t g a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a

3480 cPPT/CTS a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a

PmeIAbsI 3540 cPPT/CTS MATAAS t c a a a a t t t t a t c g a t c a c g a g a c t a g c c t c g a g g t t t a a a c a t g g c g a c c g c a g c g t c t a g t t t t a a a a t a g c t a g t g c t c t g a t c g g a g c t c c a a a t t t g t a c c g c t g g c g t c g c a g a

3600 NHYSLLTSSASIVHAEPPGG a a c c a c t a c a g c c t g c t c a c c t c c a g c g c c t c c a t c g t a c a t g c c g a g c c g c c t g g c g g c t t g g t g a t g t c g g a c g a g t g g a g g t c g c g g a g g t a g c a t g t a c g g c t c g g c g g a c c g c c g

3660 MQQGAGGYREAQSLVQGDYG a t g c a g c a g g g c g c a g g g g g c t a c c g c g a g g c g c a g a g c c t g g t g c a g g g c g a c t a c g g c t a c g t c g t c c c g c g t c c c c c g a t g g c g c t c c g c g t c t c g g a c c a c g t c c c g c t g a t g c c g

3720 ALQSNGHPLSHAHQWITALS g c g c t g c a g a g c a a c g g g c a c c c g c t c a g c c a c g c t c a c c a g t g g a t c a c c g c g c t g t c c c g c g a c g t c t c g t t g c c c g t g g g c g a g t c g g t g c g a g t g g t c a c c t a g t g g c g c g a c a g g

3780 HGGGGGGGGGGGGGGGGGGG c a c g g c g g c g g c g g c g g g g g c g g c g g c g g c g g t g g a g g a g g c g g g g g a g g c g g c g g g g g a g t g c c g c c g c c g c c g c c c c c g c c g c c g c c g c c a c c t c c t c c g c c c c c t c c g c c g c c c c c t

3840 GGDGSPWSTSPLGQPDIKPS g g c g g c g a c g g c t c c c c g t g g t c c a c c a g c c c c c t a g g c c a g c c g g a c a t c a a g c c c t c g c c g c c g c t g c c g a g g g g c a c c a g g t g g t c g g g g g a t c c g g t c g g c c t g t a g t t c g g g a g c

3900 VVVQQGGRGDELHGPGALQQ g t g g t g g t a c a g c a g g g t g g c c g a g g c g a c g a g c t g c a c g g g c c a g g a g c g c t g c a g c a a c a c c a c c a t g t c g t c c c a c c g g c t c c g c t g c t c g a c g t g c c c g g t c c t c g c g a c g t c g t t

3960 QHQQQQQQQQQQQQQQQQQQ c a g c a t c a a c a g c a a c a g c a a c a g c a g c a g c a g c a g c a g c a g c a g c a g c a g c a g c a a c a g g t c g t a g t t g t c g t t g t c g t t g t c g t c g t c g t c g t c g t c g t c g t c g t c g t c g t c g t t g t c

4020 QQQQQRPPHLVHHAANHHPG c a g c a g c a a c a a c a g c g a c c g c c a c a t c t g g t g c a c c a c g c t g c c a a c c a c c a t c c c g g g g t c g t c g t t g t t g t c g c t g g c g g t g t a g a c c a c g t g g t g c g a c g g t t g g t g g t a g g g c c c

4080 PGAWRSAAAAAHLPPSMGAS c c c g g g g c a t g g c g g a g t g c g g c g g c t g c a g c t c a c c t c c c t c c c t c c a t g g g a g c t t c c g g g c c c c g t a c c g c c t c a c g c c g c c g a c g t c g a g t g g a g g g a g g g a g g t a c c c t c g a a g g

4140 NGGLLYSQPSFTVNGMLGAG a a c g g c g g t t t g c t c t a t t c g c a g c c g a g c t t c a c g g t g a a c g g c a t g c t g g g c g c a g g a t t g c c g c c a a a c g a g a t a a g c g t c g g c t c g a a g t g c c a c t t g c c g t a c g a c c c g c g t c c t

Bsu36I 4200 GQPAGLHHHGLRDAHDEPHH g g g c a g c c g g c t g g g c t g c a c c a c c a c g g c c t g a g g g a c g c c c a c g a t g a g c c a c a c c a t c c c g t c g g c c g a c c c g a c g t g g t g g t g c c g g a c t c c c t g c g g g t g c t a c t c g g t g t g g t a

4260 ADHHPHPHSHPHQQPPPPPP g c a g a c c a c c a c c c g c a t c c g c a c t c t c a c c c a c a c c a g c a a c c g c c c c c g c c a c c t c c c c g t c t g g t g g t g g g c g t a g g c g t g a g a g t g g g t g t g g t c g t t g g c g g g g g c g g t g g a g g g

4320 PQGPPGHPGAHHDPHSDEDT c c a c a a g g c c c a c c g g g c c a c c c a g g c g c g c a c c a c g a c c c g c a c t c g g a c g a g g a c a c g g g t g t t c c g g g t g g c c c g g t g g g t c c g c g c g t g g t g c t g g g c g t g a g c c t g c t c c t g t g c

4380 PTSDDLEQFAKQFKQRRIKL c c g a c c t c a g a c g a c c t g g a g c a g t t c g c c a a g c a a t t c a a g c a g a g g c g g a t c a a a c t c g g c t g g a g t c t g c t g g a c c t c g t c a a g c g g t t c g t t a a g t t c g t c t c c g c c t a g t t t g a g

BmgBI 4440 GFTQADVGLALGTLYGNVFS g g a t t t a c t c a a g c a g a c g t g g g g c t g g c g c t t g g c a c c c t g t a c g g c a a c g t g t t c t c g c c t a a a t g a g t t c g t c t g c a c c c c g a c c g c g a a c c g t g g g a c a t g c c g t t g c a c a a g a g c

4500 QTTICRFEALQLSFKNMCKL c a g a c c a c c a t c t g c a g g t t t g a g g c c c t g c a g c t g a g c t t c a a g a a c a t g t g c a a g c t g g t c t g g t g g t a g a c g t c c a a a c t c c g g g a c g t c g a c t c g a a g t t c t t g t a c a c g t t c g a c

4560 KPLLNKWLEEADSSSGSPTS a a g c c t t t g t t g a a c a a g t g g t t g g a a g a g g c a g a c t c a t c c t c g g g c a g c c c c a c c a g c t t c g g a a a c a a c t t g t t c a c c a a c c t t c t c c g t c t g a g t a g g a g c c c g t c g g g g t g g t c g

4620 IDKIAAQGRKRKKRTSIEVS a t a g a c a a g a t c g c a g c g c a a g g g c g c a a a c g g a a a a a g c g g a c c t c c a t c g a g g t g a g c t a t c t g t t c t a g c g t c g c g t t c c c g c g t t t g c c t t t t t c g c c t g g a g g t a g c t c c a c t c g

4680 VKGALESHFLKCPKPSAQEI g t c a a g g g g g c t c t g g a g a g c c a t t t c c t c a a a t g c c c t a a g c c c t c g g c c c a g g a g a t c c a g t t c c c c c g a g a c c t c t c g g t a a a g g a g t t t a c g g g a t t c g g g a g c c g g g t c c t c t a g

4740 TSLADSLQLEKEVVRVWFCN a c c t c c c t c g c g g a c a g c t t a c a g c t g g a g a a g g a g g t g g t g a g a g t t t g g t t t t g t a a c t g g a g g g a g c g c c t g t c g a a t g t c g a c c t c t t c c t c c a c c a c t c t c a a a c c a a a a c a t t g

4800 RRQKEKRMTPPGGTLPGAED a g g a g a c a g a a a g a g a a a a g g a t g a c c c c t c c c g g a g g g a c t c t g c c g g g c g c c g a g g a t t c c t c t g t c t t t c t c t t t t c c t a c t g g g g a g g g c c t c c c t g a g a c g g c c c g c g g c t c c t a

MluI 4860 VYGGSRDTPPHHGVQTPVQT g t g t a t g g g g g t a g t a g g g a c a c g c c a c c a c a c c a c g g g g t g c a g a c g c c c g t c c a g a c g c a c a t a c c c c c a t c a t c c c t g t g c g g t g g t g t g g t g c c c c a c g t c t g c g g g c a g g t c t g c

4920 1 5 10 15 DYKDHDGDYKDHDIDY 3xFLAG RGSGDYKDHDGDYKDHDIDY c g t g g c a g c g g c g a c t a c a a a g a c c a t g a c g g t g a t t a t a a a g a t c a t g a c a t c g a t t a c g c a c c g t c g c c g c t g a t g t t t c t g g t a c t g c c a c t a a t a t t t c t a g t a c t g t a g c t a a t g

NdeISpeI 4980 20 KDDDDK * 3xFLAG WPRE KDDDDK* a a g g a t g a c g a t g a c a a g t a g a c t a g t c a t a t g a t a a t c a a c c t c t g g a t t a c a a a a t t t t t c c t a c t g c t a c t g t t c a t c t g a t c a g t a t a c t a t t a g t t g g a g a c c t a a t g t t t t a a a

5040 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c

5100 WPRE (in frame with Factor Xa site) *HRQIMSNSGTHSENEGGQ MPLYHAIASRMAFIFSSL *HRQIMSNSGTHSENEGGQ c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a

5160 WPRE (in frame with Factor Xa site) IFGPQQRKILLQPGNDPLTA YKSWLLSLYEELWPVVRQRG IFGPQQRKILLQPGNDPLTA a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c

5220 WPRE (in frame with Factor Xa site) HHASHKSVCGGSTPANGGGT VVCTVFADATPTGWGIATTC HHASHKSVCGGSTPANGGGT t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g

5280 WPRE (in frame with Factor Xa site) LEKGPSESEGERNGRRFEDG QLLSGTFAFPLPIATAELIA LEKGPSESEGERNGRRFEDG a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c

5340 WPRE (in frame with Factor Xa site) GAKGAAPCPSPQQASVIGHH ACLARCWTGARLLGTDNSVV GAKGAAPCPSPQQASVIGHH c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a

5400 WPRE (in frame with Factor Xa site) QRPLQRGKWPQEGTNGGPNQ LSGKLTSFPWLLACVATWIL QRPLQRGKWPQEGTNGGPNQ t g t c g g g g a a g c t g a c g t c c t t t c c a t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c a c a g c c c c t t c g a c t g c a g g a a a g g t a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g

5460 WPRE (in frame with Factor Xa site) APRGEAVDR 1 RGEI Factor Xa site RGTSFCYVPSALNPADLPSR APRGEAVDRRGEIWRVKRGA g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c

5520 WPRE GLLPALRPLPRLRLRPQTSR AQQRSQPRKRTKAKARLRTP g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t

SnaBIMscI*Acc65IKpnI 5580 WPRE ISLWAASPHRYVWPGTFKTN DGKPGGGRM t c t c c c t t t g g g c c g c c t c c c c g c a t c g g t a c g t a t g g c c a g g t a c c t t t a a g a c c a a t g a g a g g g a a a c c c g g c g g a g g g g c g t a g c c a t g c a t a c c g g t c c a t g g a a a t t c t g g t t a c

5640 DLQGSCRS* a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c

BamHI 5700 c t a a t t c a c t c c c a a c g a a g a c a a g a t g g g a t c a a t t c a c c a t g c t a g t g g a t c c c c c t c g a t t a a g t g a g g g t t g c t t c t g t t c t a c c c t a g t t a a g t g g t a c g a t c a c c t a g g g g g a g

5760 tetO tetO tetracycline response element g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t c c c t a t c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a g g g a t a

5820 tetO tetO tetracycline response element c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c

5880 tetO tetracycline response element a a a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t t t t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a

5940 tetO tetO tetracycline response element c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t

6000 tetO tetracycline response element a a a g t g a a a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g g g g c t t t t c a c t t t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c c c c g a

EcoRIBstBI 6060 H1 promoter g c a g g a a t t c g a a c g c t g a c g t c a t c a a c c c g c t c c a a g g a a t c g c g g g c c c a g t g t c a c c g t c c t t a a g c t t g c g a c t g c a g t a g t t g g g c g a g g t t c c t t a g c g c c c g g g t c a c a g t g

6120 H1 promoter t a g g c g g g a a c a c c c a g c g c g c g t g c g c c c t g g c a g g a a g a t g g c t g t g a g g g a c a g g g g a t c c g c c c t t g t g g g t c g c g c g c a c g c g g g a c c g t c c t t c t a c c g a c a c t c c c t g t c c c c

6180 H1 promoter T C G C T A T G T G T T C T G G G A A A H1 a g t g g c g c c c t g c a a t a t t t g c a t g t c g c t a t g t g t t c t g g g a a a t c a c c a t a a a c g t g a t c a c c g c g g g a c g t t a t a a a c g t a c a g c g a t a c a c a a g a c c c t t t a g t g g t a t t t g c a c t

6240 H1 promoter a a t g t c t t t g g a t t t g g g a a t c t t a t a a g t t c t g t a t g a g a c c a c g c g g c g t c t c c a a g c t t a c a g a a a c c t a a a c c c t t a g a a t a t t c a a g a c a t a c t c t g g t g c g c c g c a g a g g t t c g

6300 loxP t t a t c g a t a c c g t c g c a t g g g a a t a a c t t c g t a t a g c a t a c a t t a t a c g a a g t t a t g c t g a a t a g c t a t g g c a g c g t a c c c t t a t t g a a g c a t a t c g t a t g t a a t a t g c t t c a a t a c g a c

6360 5' LTR (truncated) c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g

6420 5' LTR (truncated) t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c

6480 5' LTR (truncated) t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c

XbaI 6540 5' LTR (truncated) SP6 promoter G A T A T C A C A G T G G A T T T A SP6 t g g a a a a t c t c t a g c a g c a t c t a g a a t t a a t t c c g t g t a t t c t a t a g t g t c a c c t a a a t c a c c t t t t a g a g a t c g t c g t a g a t c t t a a t t a a g g c a c a t a a g a t a t c a c a g t g g a t t t a g

BsrGI 6600 g t a t g t g t a t g a t a c a t a a g g t t a t g t a t t a a t t g t a g c c g c g t t c t a a c g a c a a t a t g t c a t a c a c a t a c t a t g t a t t c c a a t a c a t a a t t a a c a t c g g c g c a a g a t t g c t g t t a t a c a

6660 a c a a g c c t a a t t g t g t a g c a t c t g g c t t a c t g a a g c a g a c c c t a t c a t c t c t c t c g t a a a t g t t c g g a t t a a c a c a t c g t a g a c c g a a t g a c t t c g t c t g g g a t a g t a g a g a g a g c a t t t

6720 c t g c c g t c a g a g t c g g t t t g g t t g g a c g a a c c t t c t g a g t t t c t g g t a a c g c c g t c c c g c g a c g g c a g t c t c a g c c a a a c c a a c c t g c t t g g a a g a c t c a a a g a c c a t t g c g g c a g g g c g

BmtINheI 6780 a c c c g g a a a t g g t c a g c g a a c c a a t c a g c a g g g t c a t c g c t a g c c a g a t c c t c t a c g c c g t g g g c c t t t a c c a g t c g c t t g g t t a g t c g t c c c a g t a g c g a t c g g t c t a g g a g a t g c g g c

SgrAI 6840 G G A T A T A G C G G C pBRrevBam g a c g c a t c g t g g c c g g c a t c a c c g g c g c c a c a g g t g c g g t t g c t g g c g c c t a t a t c g c c g c t g c g t a g c a c c g g c c g t a g t g g c c g c g g t g t c c a c g c c a a c g a c c g c g g a t a t a g c g g c

6900 T G T A G T G G pBRrevBam a c a t c a c c g a t g g g g a a g a t c g g g c t c g c c a c t t c g g g c t c a t g a g c g c t t g t t t c g g c g t g t a g t g g c t a c c c c t t c t a g c c c g a g c g g t g a a g c c c g a g t a c t c g c g a a c a a a g c c g c

6960 t g g g t a t g g t g g c a g g c c c c g t g g c c g g g g g a c t g t t g g g c g c c a t c t c c t t g c a t g c a c a c c c a t a c c a c c g t c c g g g g c a c c g g c c c c c t g a c a a c c c g c g g t a g a g g a a c g t a c g t g

7020 c a t t c c t t g c g g c g g c g g t g c t c a a c g g c c t c a a c c t a c t a c t g g g c t g c t t c c t a a t g c g t a a g g a a c g c c g c c g c c a c g a g t t g c c g g a g t t g g a t g a t g a c c c g a c g a a g g a t t a c g

7080 A T G T T A G A C G A G A C T A C G G pRS-marker a g g a g t c g c a t a a g g g a g a g c g t c g a a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c t c c t c a g c g t a t t c c c t c t c g c a g c t t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g

7140 C pRS-marker g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a

7200 C C G G G A G C T G C A T G T G T C A G pGEX 3' c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c

7260 A G G pGEX 3' C G G A G C A C T A T G C G G A T A A pBRforEco a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a

7320 t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t

7380 AmpR promoter a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g

7440 1 signal sequence MSI AmpR promoter AmpR MSI a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a

7500 5 10 15 20 signal sequence QHFRVALIPFFAAFCLPVFA AmpR QHFRVALIPFFAAFCLPVFA c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a

7560 25 30 35 40 HPETLVKVKDAEDQLGARVG AmpR HPETLVKVKDAEDQLGARVG c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a

7620 45 50 55 60 YIELDLNSGKILESFRPEER AmpR YIELDLNSGKILESFRPEER t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a

7680 65 70 75 80 FPMMSTFKVLLCGAVLSRID AmpR C G A T A C A C C G C G C C A T A A T A Amp-R FPMMSTFKVLLCGAVLSRID t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g

7740 85 90 95 100 AGQEQLGRRIHYSQNDLVEY AmpR AGQEQLGRRIHYSQNDLVEY g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g

7800 105 110 115 120 SPVTEKHLTDGMTVRELCSA AmpR SPVTEKHLTDGMTVRELCSA t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a

PvuI 7860 125 130 135 140 AITMSDNTAANLLLTTIGGP AmpR AITMSDNTAANLLLTTIGGP g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c

7920 145 150 155 160 KELTAFLHNMGDHVTRLDRW AmpR KELTAFLHNMGDHVTRLDRW a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c

7980 165 170 175 180 EPELNEAIPNDERDTTMPVA AmpR EPELNEAIPNDERDTTMPVA g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t

FspI 8040 185 190 195 200 MATTLRKLLTGELLTLASRQ AmpR MATTLRKLLTGELLTLASRQ a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t

8100 205 210 215 220 QLIDWMEADKVAGPLLRSAL AmpR QLIDWMEADKVAGPLLRSAL c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a

8160 225 230 235 240 PAGWFIADKSGAGERGSRGI AmpR PAGWFIADKSGAGERGSRGI c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g

AhdI 8220 245 250 255 260 IAALGPDGKPSRIVVIYTTG AmpR IAALGPDGKPSRIVVIYTTG a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c

8280 265 270 275 280 SQATMDERNRQIAEIGASLI AmpR SQATMDERNRQIAEIGASLI a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a

8340 285 KHW* AmpR KHW* a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a

8400 c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g

8460 c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a

8520 ori t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t

8580 ori c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g

8640 ori t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g

8700 ori t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a

8760 ori g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a

8820 ori a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c

8880 ori a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t

8940 ori g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c

9000 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t

9060 ori c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g

9120 a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a

9180 g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a

9240 A G C G A G T C A G T G A G C G A G L4440 c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t

9300 SV40 promoter C C T T A C A C A pBABE 3' a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g t g g a a t g t g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c a c c t t a c a c a

9360 SV40 promoter C A G T C A A T C C C A pBABE 3' g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g

9420 SV40 promoter a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t

9480 SV40 promoter SV40 ori t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g

9540 SV40 promoter SV40 ori c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t

9600 SV40 promoter SV40 ori t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a

9660 SV40 promoter SV40 ori t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t t g g a c a c a a g a c a g g c t t g c g a g a t a t g t a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a a c c t g t g t t c t g t c c g a a c g c t c t a t a c a

9720 t t g a g a a t a c c a c t t t a t c c c g c g t c a g g g a g a g g c a g t g c g t a a a a a g a c g c g g a c t c a a a c t c t t a t g g t g a a a t a g g g c g c a g t c c c t c t c c g t c a c g c a t t t t t c t g c g c c t g a g t

9780 t g t g a a a t a c t g g t t t t t a g t g c g c c a g a t c t c t a t a a t c t c g c g c a a c c t a t t t t c c c c a c a c t t t a t g a c c a a a a a t c a c g c g g t c t a g a g a t a t t a g a g c g c g t t g g a t a a a a g g g g

9840 t c g a a c a c t t t t t a a g c c g t a g a t a a a c a g g c t g g g a c a c t t c a c a t g a g c g a a a a a t a c a g c t t g t g a a a a a t t c g g c a t c t a t t t g t c c g a c c c t g t g a a g t g t a c t c g c t t t t t a t g

9900 a t c g t c a c c t g g g a c a t g t t g c a g a t c c a t g c a c g t a a a c t c g c a a g c c g a c t g a t g c c t t a g c a g t g g a c c c t g t a c a a c g t c t a g g t a c g t g c a t t t g a g c g t t c g g c t g a c t a c g g a

9960 t c t g a a c a a t g g a a a g g c a t t a t t g c c g t a a g c c g t g g c g g t c t g t a c c g g g t g c g t t a c a g a c t t g t t a c c t t t c c g t a a t a a c g g c a t t c g g c a c c g c c a g a c a t g g c c c a c g c a a t g

10,020 t g g c g c g t g a a c t g g g t a t t c g t c a t g t c g a t a c c g t t t g t a t t t c c a g c t a c g a t c a c g a c c g c g c a c t t g a c c c a t a a g c a g t a c a g c t a t g g c a a a c a t a a a g g t c g a t g c t a g t g c

10,080 a c a a c c a g c g c g a g c t t a a a g t g c t g a a a c g c g c a g a a g g c g a t g g c g a a g g c t t c a t c g t g t t g g t c g c g c t c g a a t t t c a c g a c t t t g c g c g t c t t c c g c t a c c g c t t c c g a a g t a g c

10,140 t t a t t g a t g a c c t g g t g g a t a c c g g t g g t a c t g c g g t t g c g a t t c g t g a a a t g t a t c c a a a a t a a c t a c t g g a c c a c c t a t g g c c a c c a t g a c g c c a a c g c t a a g c a c t t t a c a t a g g t t

10,200 a a g c g c a c t t t g t c a c c a t c t t c g c a a a a c c g g c t g g t c g t c c g c t g g t t g a t g a c t a t g t t c g c g t g a a a c a g t g g t a g a a g c g t t t t g g c c g a c c a g c a g g c g a c c a a c t a c t g a t a c

10,260 t t g t t g a t a t c c c g c a a g a t a c c t g g a t t g a a c a g c c g t g g g a t a t g g g c g t c g t a t t c g a a c a a c t a t a g g g c g t t c t a t g g a c c t a a c t t g t c g g c a c c c t a t a c c c g c a g c a t a a g c

10,320 t c c c g c c a a t c t c c g g t c g c t a a t c t t t t c a a c g c c t g g c a c t g c c g g g c g t t g t t c t t t a g g g c g g t t a g a g g c c a g c g a t t a g a a a a g t t g c g g a c c g t g a c g g c c c g c a a c a a g a a a

10,380 t t a a c t t c a g g c g g g t t a c a a t a g t t t c c a g t a a g t a t t c t g g a g g c t g c a t c c a t g a c a a a t t g a a g t c c g c c c a a t g t t a t c a a a g g t c a t t c a t a a g a c c t c c g a c g t a g g t a c t g t

10,440 c a g g c a a a c c t g a g c g a a a c c c t g t t c a a a c c c c g c t t t a a a c a t c c t g a a a c c t c g a c g g t c c g t t t g g a c t c g c t t t g g g a c a a g t t t g g g g c g a a a t t t g t a g g a c t t t g g a g c t g c

10,500 c t a g t c c g c c g c t t t a a t c a c g g c g c a c a a c c g c c t g t g c a g t c g g c c c t t g a t g g t a a a g a t c a g g c g g c g a a a t t a g t g c c g c g t g t t g g c g g a c a c g t c a g c c g g g a a c t a c c a t t t

10,560 a c c a t c c c t c a c t g g t a t c g c a t g a t t a a c c g t c t g a t g t g g a t c t g g c g c g g c a t t g a c t g g t a g g g a g t g a c c a t a g c g t a c t a a t t g g c a g a c t a c a c c t a g a c c g c g c c g t a a c t g

10,620 c c a c g c g a a a t c c t c g a c g t c c a g g c a c g t a t t g t g a t g a g c g a t g c c g a a c g t a c c g a c g g t g c g c t t t a g g a g c t g c a g g t c c g t g c a t a a c a c t a c t c g c t a c g g c t t g c a t g g c t g

10,680 g a t g a t t t a t a c g a t a c g g t g a t t g g c t a c c g t g g c g g c a a c t g g a t t t a t g a g t g g g c c c t a c t a a a t a t g c t a t g c c a c t a a c c g a t g g c a c c g c c g t t g a c c t a a a t a c t c a c c c g g

10,740 c c g g a t c t t t g t g a a g g a a c c t t a c t t c t g t g g t g t g a c a t a a t t g g a c a a a c t a c c t a c g g c c t a g a a a c a c t t c c t t g g a a t g a a g a c a c c a c a c t g t a t t a a c c t g t t t g a t g g a t g

10,800 small t intron a g a g a t t t a a a g c t c t a a g g t a a a t a t a a a a t t t t t a a g t g t a t a a t g t g t t a a a c t a c t t c t c t a a a t t t c g a g a t t c c a t t t a t a t t t t a a a a a t t c a c a t a t t a c a c a a t t t g a t g a

10,860 small t intron g a t t c t a a t t g t t t g t g t a t t t t a g a t t c c a a c c t a t g g a a c t g a t g a a t g g g a g c a g t g c t a a g a t t a a c a a a c a c a t a a a a t c t a a g g t t g g a t a c c t t g a c t a c t t a c c c t c g t c a c

10,920 MPSSDD g t g g a a t g c c t t t a a t g a g g a a a a c c t g t t t t g c t c a g a a g a a a t g c c a t c t a g t g a t g a c a c c t t a c g g a a a t t a c t c c t t t t g g a c a a a a c g a g t c t t c t t t a c g g t a g a t c a c t a c t

10,980 130 PKKKRKV ED SV40 NLS EATADSQHSTPPKKKRKVED t g a g g c t a c t g c t g a c t c t c a a c a t t c t a c t c c t c c a a a a a a g a a g a g a a a g g t a g a a g a a c t c c g a t g a c g a c t g a g a g t t g t a a g a t g a g g a g g t t t t t t c t t c t c t t t c c a t c t t c t

11,040 (in frame with SV40 NLS) PKDFPSELLSFLSHAVFSNR PKDFPSELLSFLSHAVFSNR c c c c a a g g a c t t t c c t t c a g a a t t g c t a a g t t t t t t g a g t c a t g c t g t g t t t a g t a a t a g g g g g t t c c t g a a a g g a a g t c t t a a c g a t t c a a a a a a c t c a g t a c g a c a c a a a t c a t t a t c

11,100 (in frame with SV40 NLS) TLACFAIYTTKEKAALLYKK TLACFAIYTTKEKAALLYKK a a c t c t t g c t t g c t t t g c t a t t t a c a c c a c a a a g g a a a a a g c t g c a c t g c t a t a c a a g a a t t g a g a a c g a a c g a a a c g a t a a a t g t g g t g t t t c c t t t t t c g a c g t g a c g a t a t g t t c t t

11,160 (in frame with SV40 NLS) IMEKYSVTFISRHNSYNHNI IMEKYSVTFISRHNSYNHNI a a t t a t g g a a a a a t a t t c t g t a a c c t t t a t a a g t a g g c a t a a c a g t t a t a a t c a t a a c a t t t a a t a c c t t t t t a t a a g a c a t t g g a a a t a t t c a t c c g t a t t g t c a a t a t t a g t a t t g t a

11,220 (in frame with SV40 NLS) LFFLTPHRHRVSAINNYAQK LFFLTPHRHRVSAINNYAQK a c t g t t t t t t c t t a c t c c a c a c a g g c a t a g a g t g t c t g c t a t t a a t a a c t a t g c t c a a a a t g a c a a a a a a g a a t g a g g t g t g t c c g t a t c t c a c a g a c g a t a a t t a t t g a t a c g a g t t t t

11,280 (in frame with SV40 NLS) LCTFSFLICKGVNKEYLMYS LCTFSFLICKGVNKEYLMYS a t t g t g t a c c t t t a g c t t t t t a a t t t g t a a a g g g g t t a a t a a g g a a t a t t t g a t g t a t a g t a a c a c a t g g a a a t c g a a a a a t t a a a c a t t t c c c c a a t t a t t c c t t a t a a a c t a c a t a t c

11,340 (in frame with SV40 NLS) ALTRDHNQPYHICRGFTCFK ALTRDHNQPYHICRGFTCFK t g c c t t g a c t a g a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a c g g a a c t g a t c t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t

11,400 (in frame with SV40 NLS) KPPTPPPEPET* SV40 poly(A) signal KPPTPPPEPET* a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t

11,460 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t

11,520 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a

11,580 SV40 poly(A) signal a t c a t g t c t g g a t c a a c t g g a t a a c t c a a g c t a a c c a a a a t c a t c c c a a a c t t c c c a c c c t a g t a c a g a c c t a g t t g a c c t a t t g a g t t c g a t t g g t t t t a g t a g g g t t t g a a g g g t g g g

11,640 c a t a c c c t a t t a c c a c t g c c a a t t a c c t g t g g t t t c a t t t a c t c t a a a c c t g t g a t t c c t g t a t g g g a t a a t g g t g a c g g t t a a t g g a c a c c a a a g t a a a t g a g a t t t g g a c a c t a a g g a

3ʹ 5ʹ 11,700 c t g a a t t a t t t t c a t t t t a a a g a a a t t g t a t t t g t t a a a t a t g t a c t a c a a a c t t a g t a g g a c t t a a t a a a a g t a a a a t t t c t t t a a c a t a a a c a a t t t a t a c a t g a t g t t t g a a t c a t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.