Addgene MAPPW Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: MAPPW Sequencing Result


Map View

pBRrevBam (7668 .. 7687) M13/pUC Forward (7518 .. 7540) NdeI (7338) pRS-marker (7305 .. 7324) pGEX 3' (7183 .. 7205) pBRforEco (7127 .. 7145) Amp-R (6717 .. 6736) pBR322ori-F (5415 .. 5434) AflIII - PciI (5274) L4440 (5164 .. 5181) BspQI - SapI (5158) CAP binding site lac operator BspDI - ClaI (4236) HindIII (4229) BfuAI - BspMI (4224) SbfI (4221) AccI (4212) SalI (4211) BbsI (4146) NcoI (4026) SgrAI (7710) BsrGI (1285) pLXSN 5' (1335 .. 1357) pBABE 5' (1373 .. 1389) BglII (1412) I-SceI (1432) PaeR7I - XhoI (1537) BamHI (1563) BsaBI (2078) BsaAI (2298) PspOMI (2352) ApaI (2356) EcoRI (2380) MSCV-rev (2529 .. 2552) AgeI (2604) BlpI (2767) mPGK-F (2926 .. 2945) PacI (3002) Puro-R (3011 .. 3030) BsiWI (3067) RsrII (3127) Puro-F (3507 .. 3527) DraIII (3582) PmeI (3614) WPRE-R (3671 .. 3691) PflMI (3859) BtgZI (3920) MAPPW 7886 bp

Sequence View

60 5' LTR a a t g a a a g a c c c c a c c t g t a g g t t t g g c a a g c t a g c t t a a g t a a c g c c a t t t t g c a a g g c t t a c t t t c t g g g g t g g a c a t c c a a a c c g t t c g a t c g a a t t c a t t g c g g t a a a a c g t t c c g

120 5' LTR a t g g a a a a t a c a t a a c t g a g a a t a g a g a a g t t c a g a t c a a g g t t a g g a a c a g a g a g a c a g t a c c t t t t a t g t a t t g a c t c t t a t c t c t t c a a g t c t a g t t c c a a t c c t t g t c t c t c t g t c

180 5' LTR c a g a a t a t g g g c c a a a c a g g a t a t c t g t g g t a a g c a g t t c c t g c c c c g g c t c a g g g c c a a g t c t t a t a c c c g g t t t g t c c t a t a g a c a c c a t t c g t c a a g g a c g g g g c c g a g t c c c g g t t

240 5' LTR g a a c a g a t g g t c c c c a g a t g c g g t c c c g c c c t c a g c a g t t t c t a g a g a a c c a t c a g a t g t c t t g t c t a c c a g g g g t c t a c g c c a g g g c g g g a g t c g t c a a a g a t c t c t t g g t a g t c t a c a

300 5' LTR t t c c a g g g t g c c c c a a g g a c c t g a a a t g a c c c t g t g c c t t a t t t g a a c t a a c c a a t c a g t a a g g t c c c a c g g g g t t c c t g g a c t t t a c t g g g a c a c g g a a t a a a c t t g a t t g g t t a g t c a

360 5' LTR t c g c t t c t c g c t t c t g t t c g c g c g c t t c t g c t c c c c g a g c t c a a t a a a a g a g c c c a c a a c a g c g a a g a g c g a a g a c a a g c g c g c g a a g a c g a g g g g c t c g a g t t a t t t t c t c g g g t g t t g

420 5' LTR c c c t c a c t c g g c g c g c c a g t c c t c c g a t a g a c t g c g t c g c c c g g g t a c c c g t a t t c c c a a g g g a g t g a g c c g c g c g g t c a g g a g g c t a t c t g a c g c a g c g g g c c c a t g g g c a t a a g g g t t

480 5' LTR t a a a g c c t c t t g c t g t t t g c a t c c g a a t c g t g g a c t c g c t g a t c c t t g g g a g g g t c t c c t a t t t c g g a g a a c g a c a a a c g t a g g c t t a g c a c c t g a g c g a c t a g g a a c c c t c c c a g a g g a

540 5' LTR c a g a t t g a t t g a c t g c c c a c c t c g g g g g t c t t t c a t t t g g a g g t t c c a c c g a g a t t t g g a g t c t a a c t a a c t g a c g g g t g g a g c c c c c a g a a a g t a a a c c t c c a a g g t g g c t c t a a a c c t

600 MESV Ψ g a c c c c t g c c c a g g g a c c a c c g a c c c c c c c g c c g g g a g g t a a g c t g g c c a g c g g t c g t t t c t g g g g a c g g g t c c c t g g t g g c t g g g g g g g c g g c c c t c c a t t c g a c c g g t c g c c a g c a a a

660 MESV Ψ c g t g t c t g t c t c t g t c t t t g t g c g t g t t t g t g c c g g c a t c t a a t g t t t g c g c c t g c g t c t g c a c a g a c a g a g a c a g a a a c a c g c a c a a a c a c g g c c g t a g a t t a c a a a c g c g g a c g c a g a

720 MESV Ψ g t a c t a g t t a g c t a a c t a g c t c t g t a t c t g g c g g a c c c g t g g t g g a a c t g a c g a g t t c t g c a t g a t c a a t c g a t t g a t c g a g a c a t a g a c c g c c t g g g c a c c a c c t t g a c t g c t c a a g a c

780 MESV Ψ a a c a c c c g g c c g c a a c c c t g g g a g a c g t c c c a g g g a c t t t g g g g g c c g t t t t t g t g g c c c t t g t g g g c c g g c g t t g g g a c c c t c t g c a g g g t c c c t g a a a c c c c c g g c a a a a a c a c c g g g

840 MESV Ψ g a c c t g a g g a a g g g a g t c g a t g t g g a a t c c g a c c c c g t c a g g a t a t g t g g t t c t g g t a g g c t g g a c t c c t t c c c t c a g c t a c a c c t t a g g c t g g g g c a g t c c t a t a c a c c a a g a c c a t c c

900 MESV Ψ a g a c g a g a a c c t a a a a c a g t t c c c g c c t c c g t c t g a a t t t t t g c t t t c g g t t t g g a a c c g t c t g c t c t t g g a t t t t g t c a a g g g c g g a g g c a g a c t t a a a a a c g a a a g c c a a a c c t t g g c

960 MESV Ψ a a g c c g c g c g t c t t g t c t g c t g c a g c g c t g c a g c a t c g t t c t g t g t t g t c t c t g t c t g a c t t c g g c g c g c a g a a c a g a c g a c g t c g c g a c g t c g t a g c a a g a c a c a a c a g a g a c a g a c t g

1020 gag (truncated) t g t g t t t c t g t a t t t g t c t g a a a a t t a g g g c c a g a c t g t t a c c a c t c c c t t a a g t t t g a c a c a c a a a g a c a t a a a c a g a c t t t t a a t c c c g g t c t g a c a a t g g t g a g g g a a t t c a a a c t g

1080 gag (truncated) c t t a g g t c a c t g g a a a g a t g t c g a g c g g a t c g c t c a c a a c c a g t c g g t a g a t g t c a a g a a g a a t c c a g t g a c c t t t c t a c a g c t c g c c t a g c g a g t g t t g g t c a g c c a t c t a c a g t t c t t

1140 gag (truncated) g a g a c g t t g g g t t a c c t t c t g c t c t g c a g a a t g g c c a a c c t t t a a c g t c g g a t g g c c g c g c t c t g c a a c c c a a t g g a a g a c g a g a c g t c t t a c c g g t t g g a a a t t g c a g c c t a c c g g c g c

1200 gag (truncated) a g a c g g c a c c t t t a a c c g a g a c c t c a t c a c c c a g g t t a a g a t c a a g g t c t t t t c a c c t g g t c t g c c g t g g a a a t t g g c t c t g g a g t a g t g g g t c c a a t t c t a g t t c c a g a a a a g t g g a c c

1260 gag (truncated) c c c g c a t g g a c a c c c a g a c c a g g t c c c c t a c a t c g t g a c c t g g g a a g c c t t g g c t t t t g a g g g c g t a c c t g t g g g t c t g g t c c a g g g g a t g t a g c a c t g g a c c c t t c g g a a c c g a a a a c t

BsrGI 1320 gag (truncated) c c c c c c t c c c t g g g t c a a g c c c t t t g t a c a c c c t a a g c c t c c g c c t c c t c t t c c t c c a t c g g g g g g a g g g a c c c a g t t c g g g a a a c a t g t g g g a t t c g g a g g c g g a g g a g a a g g a g g t a g

1380 gag (truncated) C C C T T G A A C C T C C T C G T T C G A C C pLXSN 5' C T T T A T C C pBABE 5' c g c c c c g t c t c t c c c c c t t g a a c c t c c t c g t t c g a c c c c g c c t c g a t c c t c c c t t t a t c c g c g g g g c a g a g a g g g g g a a c t t g g a g g a g c a a g c t g g g g c g g a g c t a g g a g g g a a a t a g g

I-SceIBglII 1440 gag (truncated) A G C C C T C A C pBABE 5' a g c c c t c a c t c c t t c t c t a g g c g c c g g a a t t a g a t c t c t c g a c t a g g g a t a a c a g g g t a a t c g g g a g t g a g g a a g a g a t c c g c g g c c t t a a t c t a g a g a g c t g a t c c c t a t t g t c c c a t t

1500 t t g t t t g a a t g a g g c t t c a g t a c t t t a c a g a a t c g t t g c c t g c a c a t c t t g g a a a c a c t t a a c a a a c t t a c t c c g a a g t c a t g a a a t g t c t t a g c a a c g g a c g t g t a g a a c c t t t g t g a a

PaeR7IXhoI 1560 g c t g g g a t t a c t t c g a c t t c t t a a c c c a a c a g a a g g c t c g a g a a g g t a t a t t g c t g t t g a c g a c c c t a a t g a a g c t g a a g a a t t g g g t t g t c t t c c g a g c t c t t c c a t a t a a c g a c a a c t

BamHI 1620 c a g g a t c c a c a t t a a c a a c t t t a a g t c c a g c a a t t t g a g t t a a g g g t g t t g c t c t c a a t g g t c c t a g g t g t a a t t g t t g a a a t t c a g g t c g t t a a a c t c a a t t c c c a c a a c g a g a g t t a c

1680 a t t t c a c t a a t g g t t c a a t t t t t a a t t t c t t t t c t t c t g g t t c a a a a t t c a a g t t t c a a g t a a a g t g a t t a c c a a g t t a a a a a t t a a a g a a a a g a a g a c c a a g t t t t a a g t t c a a a g t t c

1740 t g a a a g t g t a a t a t g c a c c c a t t t c t t t c a a t a a a t c t t c t a a a t a g t c c a c t a a t g t t t a c t t t c a c a t t a t a c g t g g g t a a a g a a a g t t a t t t a g a a g a t t t a t c a g g t g a t t a c a a a

1800 t a t t t t g t t t t t t a c a a a a t c a a g c a g c c t c t g c t a t c a a t a t a g a a c c t t g t a t t c c a t a t a a a a c a a a a a a t g t t t t a g t t c g t c g g a g a c g a t a g t t a t a t c t t g g a a c a t a a g g t a

1860 c t t t a t c t c t a g c t g a g t c a t c a a t t a c a t a t c c a t a a c t t t c t t c a t a a g c a a a a a c a a g a a a t a g a g a t c g a c t c a g t a g t t a a t g t a t a g g t a t t g a a a g a a g t a t t c g t t t t t g t t

1920 a a t t t a a t c c g t t a t c t t c t t c t t t a g c a a t t t c t c t a c c c a t t c a t t t c a a t c c a g t t a t t a a a t t a g g c a a t a g a a g a a g a a a t c g t t a a a g a g a t g g g t a a g t a a a g t t a g g t c a a t

1980 a a g t t t t t a c a a t c t t a a c t c c a t a t t t t t c c t g a g c g a t t c t a t c a c c c a a a t c a c t t g t t c a a a a a t g t t a g a a t t g a g g t a t a a a a a g g a c t c g c t a a g a t a g t g g g t t t a g t g a a c

2040 t t a c a a a a c t t g a a t a t a g a g c c g g a t t t t t t g g a a t g c t a t t t a a g c g t t t t a g a t t t g a a t g t t t t g a a c t t a t a t c t c g g c c t a a a a a a c c t t a c g a t a a a t t c g c a a a a t c t a a a c

BsaBI 2100 a t a a t t t t c a a t c a a t t a a a a t t g g t c c t g t t t g a t t t c c a t c t a a t c t t a c a a a a t g a c t a t t a a a a g t t a g t t a a t t t t a a c c a g g a c a a a c t a a a g g t a g a t t a g a a t g t t t t a c t g

2160 c a t c a t g t t t t a t t g c c a t t c c a a a t c t g t c a g c a t c t g g g t c a t t c a t a a t a a t a a t a t g t a g t a c a a a a t a a c g g t a a g g t t t a g a c a g t c g t a g a c c c a g t a a g t a t t a t t a t t a t a

2220 c t g c a t c a t g t t t a a t a c c a t a t t c a a g c g g t a t t t t t c a t g c a g g a t c a a a t t c t g g a t g a c g t a g t a c a a a t t a t g g t a t a a g t t c g c c a t a a a a a g t a c g t c c t a g t t t a a g a c c t a

2280 t t g g a t t t a c a a c a t t t t t c a a t g t t t c a t c t t c a a a t g c a c g a t c t t c a c c c t c a a t a a a a c c t a a a t g t t g t a a a a a g t t a c a a a g t a g a a g t t t a c g t g c t a g a a g t g g g a g t t a t t

BsaAI 2340 c g t t a t a t c c t g a t t c a c g t a a t c t t t t t g g g g t a a a t t t a g t t c c t g t t c c a t t a a c t g g c a a t a t a g g a c t a a g t g c a t t a g a a a a a c c c c a t t t a a a t c a a g g a c a a g g t a a t t g a c

EcoRIPspOMIApaI 2400 c g c t a a a a a t a g g g c c c t g c c t c g g a c t t c a a g g g g c t a g a a t t c g a g c a a t t a t c t t g t g c g a t t t t t a t c c c g g g a c g g a g c c t g a a g t t c c c c g a t c t t a a g c t c g t t a a t a g a a c a

2460 t t a c t a a a a c t g a a t a c c t t g c t a t c t c t t t g a t a c a t t t t t a c a a a g c t g a a t t a a a a t a a t g a t t t t g a c t t a t g g a a c g a t a g a g a a a c t a t g t a a a a a t g t t t c g a c t t a a t t t t a

2520 PGK promoter g g t a t a a a t t a a a t c a c t t t t t t c a a t t c t a c c g g g t a g g g g a g g c g c t t t t c c c a a g g c c c a t a t t t a a t t t a g t g a a a a a a g t t a a g a t g g c c c a t c c c c t c c g c g a a a a g g g t t c c g

2580 PGK promoter C G T A C G C G A A A T C G T C G G G G C G A C MSCV-rev a g t c t g g a g c a t g c g c t t t a g c a g c c c c g c t g g g c a c t t g g c g c t a c a c a a g t g g c c t c t t c a g a c c t c g t a c g c g a a a t c g t c g g g g c g a c c c g t g a a c c g c g a t g t g t t c a c c g g a g a

AgeI 2640 PGK promoter g g c c t c g c a c a c a t t c c a c a t c c a c c g g t a g g c g c c a a c c g g c t c c g t t c t t t g g t g g c c c c g g a g c g t g t g t a a g g t g t a g g t g g c c a t c c g c g g t t g g c c g a g g c a a g a a a c c a c c g g

2700 PGK promoter c c t t c g c g c c a c c t t c t a c t c c t c c c c t a g t c a g g a a g t t c c c c c c c g c c c c g c a g c t c g g g a a g c g c g g t g g a a g a t g a g g a g g g g a t c a g t c c t t c a a g g g g g g g c g g g g c g t c g a g c

2760 PGK promoter c g t c g t g c a g g a c g t g a c a a a t g g a a g t a g c a c g t c t c a c t a g t c t c g t g c a g a t g g a c a g c a g c a c g t c c t g c a c t g t t t a c c t t c a t c g t g c a g a g t g a t c a g a g c a c g t c t a c c t g t

BlpI 2820 PGK promoter g c a c c g c t g a g c a a t g g a a g c g g g t a g g c c t t t g g g g c a g c g g c c a a t a g c a g c t t t g c t c g t g g c g a c t c g t t a c c t t c g c c c a t c c g g a a a c c c c g t c g c c g g t t a t c g t c g a a a c g a

2880 PGK promoter c c t t c g c t t t c t g g g c t c a g a g g c t g g g a a g g g g t g g g t c c g g g g g c g g g c t c a g g g g c g g g a a g c g a a a g a c c c g a g t c t c c g a c c c t t c c c c a c c c a g g c c c c c g c c c g a g t c c c c g c

2940 PGK promoter C A T T C T G C A C G C T T C mPGK-F g g c t c a g g g g c g g g g c g g g c g c c c g a a g g t c c t c c g g a g g c c c g g c a t t c t g c a c g c t t c c c g a g t c c c c g c c c c g c c c g c g g g c t t c c a g g a g g c c t c c g g g c c g t a a g a c g t g c g a a g

3000 PGK promoter A A A A G mPGK-F a a a a g c g c a c g t c t g c c g c g c t g t t c t c c t c t t c c t c a t c t c c g g g c c t t t c g a c c t t t a t t t t c g c g t g c a g a c g g c g c g a c a a g a g g a g a a g g a g t a g a g g c c c g g a a a g c t g g a a a t

PacI 3060 1 5 10 15 MTEYKPTVRLATRDDVP PuroR T A C T G G C T C A T G T T C G G G T G Puro-R MTEYKPTVRLATRDDVP a t t a a c c a c c a t g a c c g a g t a c a a g c c c a c g g t g c g c c t c g c c a c c c g c g a c g a c g t c c c t a a t t g g t g g t a c t g g c t c a t g t t c g g g t g c c a c g c g g a g c g g t g g g c g c t g c t g c a g g g

BsiWI 3120 20 25 30 35 RAVRTLAAAFADYPATRHTV PuroR RAVRTLAAAFADYPATRHTV c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a c t a c c c c g c c a c g c g c c a c a c c g t g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t g a t g g g g c g g t g c g c g g t g t g g c a

RsrII 3180 40 45 50 55 DPDRHIERVTELQELFLTRV PuroR DPDRHIERVTELQELFLTRV c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g c a a g a a c t c t t c c t c a c g c g c g t g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a c g t t c t t g a g a a g g a g t g c g c g c a

3240 60 65 70 75 GLDIGKVWVADDGAAVAVWT PuroR GLDIGKVWVADDGAAVAVWT c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g g c g c c g c g g t g g c g g t c t g g a c g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t g c c g c g g c g c c a c c g c c a g a c c t g

3300 80 85 90 95 TPESVEAGAVFAEIGPRMAE PuroR TPESVEAGAVFAEIGPRMAE c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c c g a g a t c g g c c c g c g c a t g g c c g a g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c t a g c c g g g c g c g t a c c g g c t

3360 100 105 110 115 LSGSRLAAQQQMEGLLAPHR PuroR LSGSRLAAQQQMEGLLAPHR g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g g a a g g c c t c c t g g c g c c g c a c c g c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a c c t t c c g g a g g a c c g c g g c g t g g c

3420 120 125 130 135 PKEPAWFLATVGVSPDHQGK PuroR PKEPAWFLATVGVSPDHQGK g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c g t c t c g c c c g a c c a c c a g g g c a a c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c g c a g a g c g g g c t g g t g g t c c c g t t

3480 140 145 150 155 GLGSAVVLPGVEAAERAGVP PuroR GLGSAVVLPGVEAAERAGVP g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g g c g g c c g a g c g c g c c g g g g t g c c c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t c c g c c g g c t c g c g c g g c c c c a c g g

3540 160 165 170 175 AFLETSAPRNLPFYERLGFT PuroR G C A A C C T C C C C T T C T A C G A G C Puro-F AFLETSAPRNLPFYERLGFT c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c t t c t a c g a g c g g c t c g g c t t c a c g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g g a a g a t g c t c g c c g a g c c g a a g t g

DraIII 3600 180 185 190 195 VTADVEVPEGPRTWCMTRKP PuroR VTADVEVPEGPRTWCMTRKP c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g c a c c t g g t g c a t g a c c c g c a a g c c g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c g t g g a c c a c g t a c t g g g c g t t c g g

PmeI 3660 200 GA* PuroR GA* c g g t g c c t g a g t t t a a a c a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a g c c a c g g a c t c a a a t t t g t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t

3720 *HRQI A C A A C G A G G A A A A T G C G A T A C WPRE-R MPLY *HRQI t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g

3780 (in frame with Factor Xa site) MSNSGTHSENEGGQIFGPQQ HAIASRMAFIFSSLYKSWLL MSNSGTHSENEGGQIFGPQQ a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a

3840 (in frame with Factor Xa site) RKILLQPGNDPLTAHHASHK SLYEELWPVVRQRGVVCTVF RKILLQPGNDPLTAHHASHK c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c

PflMI 3900 (in frame with Factor Xa site) SVCGGSTPANGGGTLEKGPS ADATPTGWGIATTCQLLSGT SVCGGSTPANGGGTLEKGPS c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a

BtgZI 3960 (in frame with Factor Xa site) ESEGERNGRRFEDGGAKGAA FAFPLPIATAELIAACLARC ESEGERNGRRFEDGGAKGAA t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a

4020 (in frame with Factor Xa site) PCPSPQQASVIGHHQRPLQR WTGARLLGTDNSVVLSGKLT PCPSPQQASVIGHHQRPLQR g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a g c t g a c g t c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t c g a c t g c a

NcoI 4080 (in frame with Factor Xa site) GKWPQEGTNGGPNQAPRGEA SFPWLLACVATWILRGTSFC GKWPQEGTNGGPNQAPRGEA c c t t t c c a t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t g g a a a g g t a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a

4140 VDR 1 RGEI Factor Xa site YVPSALNPADLPSRGLLPAL VDRRGEIWRVKRGAAQQRSQ a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g

BbsI 4200 RPLPRLRLRPQTSRISLWAA PRKRTKAKARLRTPDGKPGG g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a

BspDIClaIHindIIISbfIBspMIBfuAISalIAccI 4260 SPPGSTCRHASLSIK* GRRPRGAPM c c c c g c c t g g g t c g a c c t g c a g g c a t g c a a g c t t a t c g a t a a a a t a a a a g a t t t t a t t t a g g g g c g g a c c c a g c t g g a c g t c c g t a c g t t c g a a t a g c t a t t t t a t t t t c t a a a a t a a a t

4320 3' LTR g t c t c c a g a a a a a g g g g g g a a t g a a a g a c c c c a c c t g t a g g t t t g g c a a g c t a g c t t a a g c a g a g g t c t t t t t c c c c c c t t a c t t t c t g g g g t g g a c a t c c a a a c c g t t c g a t c g a a t t c

4380 3' LTR t a a c g c c a t t t t g c a a g g c a t g g a a a a t a c a t a a c t g a g a a t a g a g a a g t t c a g a t c a a g a t t g c g g t a a a a c g t t c c g t a c c t t t t a t g t a t t g a c t c t t a t c t c t t c a a g t c t a g t t c

4440 3' LTR g t t a g g a a c a g a g a g a c a g c a g a a t a t g g g c c a a a c a g g a t a t c t g t g g t a a g c a g t t c c c a a t c c t t g t c t c t c t g t c g t c t t a t a c c c g g t t t g t c c t a t a g a c a c c a t t c g t c a a g g

4500 3' LTR t g c c c c g g c t c a g g g c c a a g a a c a g a t g g t c c c c a g a t g c g g t c c c g c c c t c a g c a g t t t a c g g g g c c g a g t c c c g g t t c t t g t c t a c c a g g g g t c t a c g c c a g g g c g g g a g t c g t c a a a

4560 3' LTR c t a g a g a a c c a t c a g a t g t t t c c a g g g t g c c c c a a g g a c c t g a a a t g a c c c t g t g c c t t a g a t c t c t t g g t a g t c t a c a a a g g t c c c a c g g g g t t c c t g g a c t t t a c t g g g a c a c g g a a t

4620 3' LTR t t t g a a c t a a c c a a t c a g t t c g c t t c t c g c t t c t g t t c g c g c g c t t c t g c t c c c c g a g c t a a a c t t g a t t g g t t a g t c a a g c g a a g a g c g a a g a c a a g c g c g c g a a g a c g a g g g g c t c g a

4680 3' LTR c a a t a a a a g a g c c c a c a a c c c c t c a c t c g g c g c g c c a g t c c t c c g a t a g a c t g c g t c g c c g t t a t t t t c t c g g g t g t t g g g g a g t g a g c c g c g c g g t c a g g a g g c t a t c t g a c g c a g c g g

4740 3' LTR c g g g t a c c c g t g t a t c c a a t a a a c c c t c t t g c a g t t g c a t c c g a c t t g t g g t c t c g c t g t g c c c a t g g g c a c a t a g g t t a t t t g g g a g a a c g t c a a c g t a g g c t g a a c a c c a g a g c g a c a

4800 3' LTR t c c t t g g g a g g g t c t c c t c t g a g t g a t t g a c t a c c c g t c a g c g g g g g t c t t t c a t g g g t a a g g a a c c c t c c c a g a g g a g a c t c a c t a a c t g a t g g g c a g t c g c c c c c a g a a a g t a c c c a t

4860 a c a g t t t c t t g a a g t t g g a g a a c a a c a t t c t g a g g g t a g g a g t c g a a t a t t a a g t a a t c c t g t c a a a g a a c t t c a a c c t c t t g t t g t a a g a c t c c c a t c c t c a g c t t a t a a t t c a t t a g g

4920 t g a c t c a a t t a g c c a c t g t t t t g a a t c c a c a t a c t c c a a t a c t c c t g a a a t a g t t c a t t a a c t g a g t t a a t c g g t g a c a a a a c t t a g g t g t a t g a g g t t a t g a g g a c t t t a t c a a g t a a t

4980 lac operator t g g a c a g c g c a g a a a g a g c t g g g g a g a a t t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a a c c t g t c g c g t c t t t c t c g a c c c c t c t t a a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t

5040 -10 -35 lac promoter CAP binding site c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a

5100 CAP binding site c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a

BspQISapI 5160 g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g

5220 G A G C G A G T G A C T G A G C G A L4440 t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t

AflIIIPciI 5280 c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c

5340 ori a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t

5400 ori t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t

5460 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g

5520 ori t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g

5580 ori g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a

5640 ori g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c

5700 ori t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c

5760 ori g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t

5820 ori c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c

5880 ori a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a

5940 ori t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a

6000 t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c

6060 a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a

6120 285 280 *WHKILSAG AmpR *WHKILSAG c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g

6180 275 270 265 260 IEAIQRNREDMTAQSGTTYI AmpR IEAIQRNREDMTAQSGTTYI t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a

6240 255 250 245 240 VVIRSPKGDPGLAAIIGRSG AmpR VVIRSPKGDPGLAAIIGRSG a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g

6300 235 230 225 220 REGAGSKDAIFWGAPLASRL AmpR REGAGSKDAIFWGAPLASRL a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c

6360 215 210 205 200 LPGAVKDAEMWDILQQRSAL AmpR LPGAVKDAEMWDILQQRSAL a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c

6420 195 190 185 180 TLLEGTLLKRLTTAMAVPMT AmpR TLLEGTLLKRLTTAMAVPMT a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a

6480 175 170 165 160 TDREDNPIAENLEPEWRDLR AmpR TDREDNPIAENLEPEWRDLR g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c

6540 155 150 145 140 TVHDGMNHLFATLEKPGGIT AmpR TVHDGMNHLFATLEKPGGIT a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a

6600 135 130 125 120 TLLLNAATNDSMTIAASCLE AmpR TLLLNAATNDSMTIAASCLE t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g

6660 115 110 105 100 RVTMGDTLHKETVPSYEVLD AmpR RVTMGDTLHKETVPSYEVLD t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g

6720 95 90 85 80 NQSYHIRRGLQEQGADIRSL AmpR A T A A Amp-R NQSYHIRRGLQEQGADIRSL a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t

6780 75 70 65 60 VAGCLLVKFTSMMPFREEPR AmpR T A C C G C G C C A C A T A G C Amp-R VAGCLLVKFTSMMPFREEPR t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c

6840 55 50 45 40 FSELIKGSNLDLEIYGVRAG AmpR FSELIKGSNLDLEIYGVRAG a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g

6900 35 30 25 LQDEADKVKVLTEPH 20 signal sequence AFVPL AmpR LQDEADKVKVLTEPHAFVPL c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c

6960 15 10 5 1 signal sequence CFAAFFPILAVRFHQISM AmpR AmpR promoter CFAAFFPILAVRFHQISM g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a

7020 AmpR promoter c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a

7080 AmpR promoter t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g

7140 A A T A G G C G T A T C A C pBRforEco a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g

7200 G A G G C pBRforEco G G A G A C T G T G T A C G T C G A pGEX 3' g a g g c c c t t t c g t c t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c t c c g g g a a a g c a g a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a

7260 G G G C C pGEX 3' c c c g g a g a c g g t c a c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g g g g c c t c t g c c a g t g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c

7320 C G G C A T C A G A G C A G A T pRS-marker c g c g t c a g c g g g t g t t g g c g g g t g t c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t g c g c a g t c g c c c a c a a c c g c c c a c a g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a

NdeI 7380 T G T A pRS-marker t g t a c t g a g a g t g c a c c a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a a c a t g a c t c t c a c g t g g t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t

7440 c c g c a t c a g g c g c c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c g t a g t c c g c g g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c

7500 g g c c t c t t c g c t a t t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g c c g g a g a a g c g a t a a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c

7560 C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward g g t a a c g c c a g g g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c g c a a g g a a t g g t g c a c c a t t g c g g t c c c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g c g t t c c t t a c c a c g t

7620 t g c a a g g a g a t g g c g c c c a a c a g t c c c c c g g c c a c g g g g c c t g c c a c c a t a c c c a c g c c g a c g t t c c t c t a c c g c g g g t t g t c a g g g g g c c g g t g c c c c g g a c g g t g g t a t g g g t g c g g c

7680 G G T G A T G T C G G C G pBRrevBam a a a c a a g c g c t c a t g a g c c c g a a g t g g c g a g c c c g a t c t t c c c c a t c g g t g a t g t c g g c g t t t g t t c g c g a g t a c t c g g g c t t c a c c g c t c g g g c t a g a a g g g g t a g c c a c t a c a g c c g c

SgrAI 7740 A T A T A G G pBRrevBam a t a t a g g c g c c a g c a a c c g c a c c t g t g g c g c c g g t g a t g c c g g c c a c g a t g c g t c c g g c g t a t a t c c g c g g t c g t t g g c g t g g a c a c c g c g g c c a c t a c g g c c g g t g c t a c g c a g g c c g c

7800 t a g a g g c g a t t a g t c c a a t t t g t t a a a g a c a g g a t a t c a g t g g t c c a g g c t c t a g t t t t g a t c t c c g c t a a t c a g g t t a a a c a a t t t c t g t c c t a t a g t c a c c a g g t c c g a g a t c a a a a c

7860 a c t c a a c a a t a t c a c c a g c t g a a g c c t a t a g a g t a c g a g c c a t a g a t a a a a t a a a a g a t t t g a g t t g t t a t a g t g g t c g a c t t c g g a t a t c t c a t g c t c g g t a t c t a t t t t a t t t t c t a a

7886 t t a t t t a g t c t c c a g a a a a a g g g g g g a a t a a a t c a g a g g t c t t t t t c c c c c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.