Addgene pAXY0019 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pAXY0019 Sequencing Result


Map View

pBR322ori-F (4749 .. 4768) PciI (4608) L4440 (4498 .. 4515) BspQI - SapI (4492) CAP binding site M13/pUC Reverse (4280 .. 4302) lac operator M13 Reverse (4267 .. 4283) M13 rev T3 (4228 .. 4248) BssHII (4247) KpnI (4214) Acc65I (4210) SacII (4189) EagI - NotI (4183) MfeI (4124) HpaI (4115) SV40pA-R (4059 .. 4078) EBV-rev (4005 .. 4024) SnaBI (3977) XbaI (3952) 3xHA PasI (3711) BspDI - ClaI (3488) XcmI (3436) PflMI (3375) Bsu36I (3257) NcoI (3197) PpuMI (3147) PfoI (3065) EcoRV (2943) BsaBI * (2899) BclI * (2894) BamHI (2884) SP6 (2840 .. 2857) HindIII (2833) AhdI (520) Amp-R (1070 .. 1089) XmnI (1119) F1ori-F (1575 .. 1596) DraIII (1662) F1ori-R (1787 .. 1806) SalI (1964) AccI (1965) NdeI (2324) pAXY0019 4981 bp

Sequence View

60 ori t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g

120 ori a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g

180 ori a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t

240 ori g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g

300 ori a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c

360 g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t

420 a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a

480 285 280 *WHKILSAGIE AmpR *WHKILSAGIE a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t

AhdI 540 275 270 265 260 AIQRNREDMTAQSGTTYIVV AmpR AIQRNREDMTAQSGTTYIVV g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c

600 255 250 245 240 IRSPKGDPGLAAIIGRSGGK AmpR IRSPKGDPGLAAIIGRSGGK a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c c c t t g t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g g g a a c

660 235 230 225 220 SAGSKDAIFWGAPLASRLLP AmpR SAGSKDAIFWGAPLASRLLP g a g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c t c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a

720 215 210 205 200 GAVKDAEMWDILQQRSALTL AmpR GAVKDAEMWDILQQRSALTL c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a

780 195 190 185 180 LEGTLLKRLTTAMAVPMTTD AmpR LEGTLLKRLTTAMAVPMTTD a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t

840 175 170 165 160 REDNPIAENLEPEWRDLRTV AmpR REDNPIAENLEPEWRDLRTV c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t

900 155 150 145 140 HDGMNHLFATLEKPGGITTL AmpR HDGMNHLFATLEKPGGITTL t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t

960 135 130 125 120 LLNAATNDSMTIAASCLERV AmpR LLNAATNDSMTIAASCLERV a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a

1020 115 110 105 100 TMGDTLHKETVPSYEVLDNQ AmpR TMGDTLHKETVPSYEVLDNQ g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t

1080 95 90 85 80 SYHIRRGLQEQGADIRSLVA AmpR A T A A T A C C G C G Amp-R SYHIRRGLQEQGADIRSLVA g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c

XmnI 1140 75 70 65 60 GCLLVKFTSMMPFREEPRFS AmpR C C A C A T A G C Amp-R GCLLVKFTSMMPFREEPRFS c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g

1200 55 50 45 40 ELIKGSNLDLEIYGVRAGLQ AmpR ELIKGSNLDLEIYGVRAGLQ t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t

1260 35 30 25 DEADKVKVLTEPH 20 signal sequence AFVPLCF AmpR DEADKVKVLTEPHAFVPLCF t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a

1320 15 10 5 1 signal sequence AAFFPILAVRFHQISM AmpR AmpR promoter AAFFPILAVRFHQISM g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a

1380 AmpR promoter c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a

1440 AmpR promoter f1 ori a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t a a a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a t t t

1500 f1 ori t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a

1560 f1 ori t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c

1620 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c

DraIII 1680 f1 ori t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a

1740 f1 ori c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g

1800 f1 ori A G G G A A G A A A G C G A F1ori-R g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t

1860 f1 ori A A G G A G F1ori-R a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g

1920 f1 ori c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c c c a t t c g c c a t t c a g g c t g c g c a a c t g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g g g t a a g c g g t a a g t c c g a c g c g t t g a

SalIAccI 1980 g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a c g c c a g t c g a c c g c c a a t t c a a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t g c g g t c a g c t g g c g g t t a a g t t

2040 t a t g g c g t a t a t g g a c t c a t g c c a a t t c a a t a t g g t g g a t c t g g a c c t g t g c c a a t t c a a a t a c c g c a t a t a c c t g a g t a c g g t t a a g t t a t a c c a c c t a g a c c t g g a c a c g g t t a a g t t

2100 t a t g g c g t a t a t g g a c t c g t g c c a a t t c a a t a t g g t g g a t c t g g a c c c c a g c c a a t t c a a a t a c c g c a t a t a c c t g a g c a c g g t t a a g t t a t a c c a c c t a g a c c t g g g g t c g g t t a a g t t

2160 t a t g g c g g a c t t g g c a c c a t g c c a a t a t g g c t a t t g g c c a g g t t c a a t a c t a t g t a t t g g a t a c c g c c t g a a c c g t g g t a c g g t t a t a c c g a t a a c c g g t c c a a g t t a t g a t a c a t a a c c

2220 c c c t a t g c c a t a t a g t a t t c c a t a t a t g g g t t t t c c t a t t g a c g t a g a t a g c c c c t c c c a g g g a t a c g g t a t a t c a t a a g g t a t a t a c c c a a a a g g a t a a c t g c a t c t a t c g g g g a g g g t

2280 a t g g g c g g t c c c a t a t a c c a t a t a t g g g g c t t c c t a a t a c c g c c c a t a g c c a c t c c c c c a t a c c c g c c a g g g t a t a t g g t a t a t a c c c c g a a g g a t t a t g g c g g g t a t c g g t g a g g g g g t

NdeI 2340 t t g a c g t c a a t g g t c t c t a t a t a t g g t c t t t c c t a t t g a c g t c a t a t g g g c g g t c c t a t t a a c t g c a g t t a c c a g a g a t a t a t a c c a g a a a g g a t a a c t g c a g t a t a c c c g c c a g g a t a a

2400 g a c g t a t a t g g c g c c t c c c c c a t t g a c g t c a a t t a c g g t a a a t g g c c c g c c t g g c t c a a t c t g c a t a t a c c g c g g a g g g g g t a a c t g c a g t t a a t g c c a t t t a c c g g g c g g a c c g a g t t a

2460 g c c c a t t g a c g t c a a t a g g a c c a c c c a c c a t t g a c g t c a a t g g g a t g g c t c a t t g c c c a t c g g g t a a c t g c a g t t a t c c t g g t g g g t g g t a a c t g c a g t t a c c c t a c c g a g t a a c g g g t a

2520 t c a t a t c c g t t c t c a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c a c t t g g c a g t a g t a t a g g c a a g a g t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g t g a a c c g t c a

2580 a c a t c a a t a t c t a t t a a t a g t a a c t t g g c a a g t a c a t t a c t a t t g g a a g t a c g c c a g g g t t g t a g t t a t a g a t a a t t a t c a t t g a a c c g t t c a t g t a a t g a t a a c c t t c a t g c g g t c c c a

2640 a c a t t g g c a g t a c t c c c a t t g a c g t c a a t g g c g g t a a a t g g c c c g c g a t g g c t g c c a a g t t g t a a c c g t c a t g a g g g t a a c t g c a g t t a c c g c c a t t t a c c g g g c g c t a c c g a c g g t t c a

2700 a c a t c c c c a t t g a c g t c a a t g g g g a g g g g c a a t g a c g c a a a t g g g c g t t c c a t t g a c g t a t g t a g g g g t a a c t g c a g t t a c c c c t c c c c g t t a c t g c g t t t a c c c g c a a g g t a a c t g c a t

2760 (in frame with Factor Xa site) *ILLARKPFR MGGLYKQCSFREP a a t g g g c g g t a g g c g t g c c t a a t g g g a g g t c t a t a t a a g c a a t g c t c g t t t a g g g a a c c g t t a c c c g c c a t c c g c a c g g a t t a c c c t c c a g a t a t a t t c g t t a c g a g c a a a t c c c t t g g c

2820 (in frame with Factor Xa site) WEAQPR 1 RGEI Factor Xa site PFCLGTSAFDQLAPLVHSAW c c a t t c t g c c t g g g g a c g t c g g c c t t c g a t c a a c t c g c g c c g c t g g t c c a t t c t g c c t g g g g t a a g a c g g a c c c c t g c a g c c g g a a g c t a g t t g a g c g c g g c g a c c a g g t a a g a c g g a c c

HindIII 2880 SP6 promoter A T T T A G G T G A C A C T A T A G SP6 GRRSKLDLGDTIEYKLLVLF g g a c g t c g g a g c a a g c t t g a t t t a g g t g a c a c t a t a g a a t a c a a g c t a c t t g t t c t t t t t c c t g c a g c c t c g t t c g a a c t a a a t c c a c t g t g a t a t c t t a t g t t c g a t g a a c a a g a a a a a

BsaBI*BclI*BamHI 2940 AGSTMIKIATRKYLGKQNVY g c a g g a t c c a c c a t g a t c a a g a t c g c c a c c c g g a a a t a c c t c g g c a a a c a g a a c g t g t a t c g t c c t a g g t g g t a c t a g t t c t a g c g g t g g g c c t t t a t g g a g c c g t t t g t c t t g c a c a t a

EcoRV 3000 DIGVERDHNFALKNGFIASN g a t a t c g g g g t g g a g a g g g a t c a t a a c t t c g c c c t c a a a a a t g g g t t t a t t g c c t c t a a t c t a t a g c c c c a c c t c t c c c t a g t a t t g a a g c g g g a g t t t t t a c c c a a a t a a c g g a g a t t a

3060 CADGFYKDRYVYRHFASAAL t g t g c c g a c g g t t t c t a c a a a g a t c g g t a t g t c t a t c g g c a t t t c g c a t c t g c c g c a c t g a c a c g g c t g c c a a a g a t g t t t c t a g c c a t a c a g a t a g c c g t a a a g c g t a g a c g g c g t g a c

PfoI 3120 PIPEVLDIGEFSESLTYCIS c c t a t c c c g g a g g t g c t g g a c a t a g g t g a a t t t a g t g a a t c a c t g a c t t a t t g c a t c t c c g g a t a g g g c c t c c a c g a c c t g t a t c c a c t t a a a t c a c t t a g t g a c t g a a t a a c g t a g a g g

PpuMI 3180 RRSQGVTLQDLPETELPAVL c g c a g a a g c c a a g g c g t g a c t c t t c a g g a c c t t c c a g a g a c t g a a c t c c c c g c c g t c c t g g c g t c t t c g g t t c c g c a c t g a g a a g t c c t g g a a g g t c t c t g a c t t g a g g g g c g g c a g g a c

NcoI 3240 QPVAEAMDAIAAADLSQTSG c a g c c t g t t g c t g a g g c c a t g g a c g c c a t a g c c g c c g c c g a c c t g a g c c a g a c c t c t g g t g t c g g a c a a c g a c t c c g g t a c c t g c g g t a t c g g c g g c g g c t g g a c t c g g t c t g g a g a c c a

Bsu36I 3300 FGPFGPQGIGQYTTWRDFIC t t t g g g c c t t t c g g c c c t c a g g g a a t t g g c c a g t a c a c c a c c t g g a g g g a c t t t a t t t g c a a a c c c g g a a a g c c g g g a g t c c c t t a a c c g g t c a t g t g g t g g a c c t c c c t g a a a t a a a c g

3360 AIADPHVYHWQTVMDDTVSA g c c a t c g c c g a c c c c c a t g t g t a t c a c t g g c a g a c c g t t a t g g a c g a t a c c g t t t c c g c a c g g t a g c g g c t g g g g g t a c a c a t a g t g a c c g t c t g g c a a t a c c t g c t a t g g c a a a g g c g t

PflMI 3420 SVAQALDELMLWAEDCPEVR a g c g t g g c c c a a g c t c t g g a t g a g c t g a t g c t g t g g g c g g a g g a t t g t c c c g a a g t c a g g t c g c a c c g g g t t c g a g a c c t a c t c g a c t a c g a c a c c c g c c t c c t a a c a g g g c t t c a g t c c

XcmI 3480 HLVHADFGSNNVLTDNGRIT c a t c t t g t c c a c g c c g a c t t t g g a t c t a a c a a t g t c c t g a c t g a t a a c g g c a g a a t c a c t g t a g a a c a g g t g c g g c t g a a a c c t a g a t t g t t a c a g g a c t g a c t a t t g c c g t c t t a g t g a

BspDIClaI 3540 AVIDWSEAMFGDSQYEVANI g c c g t a a t c g a t t g g t c a g a g g c c a t g t t c g g c g a c t c t c a g t a c g a g g t g g c t a a c a t c c g g c a t t a g c t a a c c a g t c t c c g g t a c a a g c c g c t g a g a g t c a t g c t c c a c c g a t t g t a g

3600 FFWRPWLACMEQQTRYFERR t t c t t t t g g a g a c c g t g g t t g g c c t g t a t g g a a c a g c a a a c a a g a t a c t t c g a g c g c c g g a a g a a a a c c t c t g g c a c c a a c c g g a c a t a c c t t g t c g t t t g t t c t a t g a a g c t c g c g g c c

3660 HPELAGSPRLRAYMLRIGLD c a c c c g g a a c t g g c c g g a t c t c c g a g g c t g a g g g c c t a c a t g c t g a g g a t a g g a c t g g a c g t g g g c c t t g a c c g g c c t a g a g g c t c c g a c t c c c g g a t g t a c g a c t c c t a t c c t g a c c t g

PasI 3720 QLYQSLVDGNFDDAAWAQGR c a g c t c t a t c a g t c c c t g g t g g a t g g a a a t t t c g a c g a t g c g g c t t g g g c c c a g g g c c g g g t c g a g a t a g t c a g g g a c c a c c t a c c t t t a a a g c t g c t a c g c c g a a c c c g g g t c c c g g c c

3780 CDAIVRSGAGTVGRTQIARR t g c g a c g c a a t a g t g c g g a g c g g a g c t g g g a c a g t g g g c c g g a c c c a g a t t g c c c g g c g a a c g c t g c g t t a t c a c g c c t c g c c t c g a c c c t g t c a c c c g g c c t g g g t c t a a c g g g c c g c t

3840 SAAVWTDGCVEVLADSGNRR t c t g c a g c t g t g t g g a c t g a t g g c t g t g t t g a g g t g c t g g c t g a c a g c g g c a a t c g g a g g a g a c g t c g a c a c a c c t g a c t a c c g a c a c a a c t c c a c g a c c g a c t g t c g c c g t t a g c c t c c

3900 1 5 10 YPYDVPDYAYP 3xHA PSTRPRAKKYPYDVPDYAYP c c c a g t a c g c g a c c t a g a g c a a a a a a a t a c c c a t a c g a t g t t c c a g a t t a c g c t t a t c c t g g g t c a t g c g c t g g a t c t c g t t t t t t t a t g g g t a t g c t a c a a g g t c t a a t g c g a a t a g g a

XbaI 3960 15 20 25 YDVPDYAYPYDVPDYA * 3xHA YDVPDYAYPYDVPDYA* t a t g a c g t a c c t g a c t a t g c a t a c c c t t a t g a t g t a c c a g a c t a c g c t t a g t c t a g a a c t a t a c t g c a t g g a c t g a t a c g t a t g g g a a t a c t a c a t g g t c t g a t g c g a a t c a g a t c t t g a

SnaBI 4020 SV40 poly(A) signal C T A C T C A A A C C T G T T T EBV-rev a t a g t g a g t c g t a t t a c g t a g a t c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a t a t c a c t c a g c a t a a t g c a t c t a g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t

4080 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R G G T G EBV-rev c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a

MfeIHpaI 4140 SV40 poly(A) signal t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t

SacIIEagINotI 4200 t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t a a t t c g c g g c c g c g g c g c c a a t g c a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a a t t a a g c g c c g g c g c c g c g g t t a c g

BssHIIAcc65IKpnI 4260 T3 promoter G G A A A T C A C T C C C A A T T A A C G T3 a t t g g g c c c g g t a c c c a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t g c g c g c t t g g c g t a t a a c c c g g g c c a t g g g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a c g c g c g a a c c g c a t

4320 -10 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a

4380 -35 lac promoter CAP binding site a c g a g c c g g g a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t t g c t c g g c c c t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a

4440 CAP binding site a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t

BspQISapI 4500 G A G L4440 a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g

4560 C G A G T G A C T G A G C G A L4440 g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t

PciI 4620 g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t

4680 ori a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a

4740 ori c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g

4800 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g

4860 ori g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g

4920 ori t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c

4980 ori t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t

4981 ori g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.