Addgene TFORF3440 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: TFORF3440 Sequencing Result


Map View

T3 (9267 .. 9287) M13 rev M13 Reverse (9232 .. 9248) lac operator M13/pUC Reverse (9213 .. 9235) CAP binding site L4440 (9000 .. 9017) pBR322ori-F (8747 .. 8766) AhdI (8014) ScaI (7533) Amp-R (7445 .. 7464) F1ori-F (6938 .. 6959) F1ori-R (6728 .. 6747) M13/pUC Forward (6492 .. 6514) M13 Forward (6483 .. 6500) M13 fwd T7 (6454 .. 6473) T7 promoter EBV-rev (6229 .. 6248) SV40pA-R (6175 .. 6194) WPRE-R (5220 .. 5240) SpeI (5066) EcoRV (5011) attB2 XcmI (4799) T3 promoter 5' LTR (truncated) HIV-1 Ψ NotI (922) AleI (1351) KflI - PpuMI (1708) NsiI (1718) Acc65I (2001) KpnI (2005) Puro-R (2020 .. 2039) PflFI - Tth111I (2062) BsiWI (2076) BspEI * (2133) RsrII (2136) BstEII (2154) MscI * (2395) Puro-F (2516 .. 2536) HpaI (2620) cPPT/CTS PspXI (2825) SbfI (2837) AgeI (2998) PspOMI (3533) ApaI (3537) FseI (3624) EF1a-F (4046 .. 4066) NheI (4100) BmtI (4104) BspDI - ClaI (4107) BclI * (4112) BsaBI * (4412) PmlI (4471) TFORF3440 9310 bp

Sequence View

60 RSV promoter t t a a t g t a g t c t t a t g c a a t a c t c t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g c a a a a t t a c a t c a g a a t a c g t t a t g a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c g t t

120 RSV promoter c a t g c c t t a c a a g g a g a g a a a a a g c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g t a c g g a a t g t t c c t c t c t t t t t c g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c a t g

180 RSV promoter g a t c g t g c c t t a t t a g g a a g g c a a c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t g a a c t a g c a c g g a a t a a t c c t t c c g t t g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a c t t

240 RSV promoter 5' LTR (truncated) t t g c c g c a t t g c a g a g a t a t t g t a t t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t c t c t c a a c g g c g t a a c g t c t c t a t a a c a t a a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a g a g a g

300 5' LTR (truncated) t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c

360 5' LTR (truncated) c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a

420 5' LTR (truncated) g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g

480 HIV-1 Ψ c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g

540 HIV-1 Ψ t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a

600 HIV-1 Ψ t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t

660 g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a

720 t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a

780 t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c

840 g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t

900 g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t

NotI 960 MR g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c

1020 DNWRSELYKYKVVKIEPLGV g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t

1080 RRE APTKAKRRVVQREKRAVGIG g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t

1140 RRE ALFLGFLGAAGSTMGAASMT g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c

1200 RRE LTVQARQLLSGIVQQQNNLL c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c

1260 RRE RAIEAQQHLLQLTVWGIKQL a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g

1320 RRE QARILAVERYLKDQQLLGIW c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c

AleI 1380 GCSGKLICTTAVPWNASWSN g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a

1440 KSLEQIWNHTTWMEWDREIN a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g

1500 1 KN gp41 peptide NYTSLIHSLIEESQNQQEKN a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a

1560 5 10 15 EQELLELDKWASL (in frame with gp41 peptide) WNWFNIT gp41 peptide EQELLELDKWASLWNWFNIT g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t

1620 (in frame with gp41 peptide) NWLWYIKLFIMIVGGLVGLR NWLWYIKLFIMIVGGLVGLR a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t

1680 (in frame with gp41 peptide) IVFAVLSIVNRVRQGYSPLS IVFAVLSIVNRVRQGYSPLS a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c

NsiIPpuMIKflI 1740 (in frame with gp41 peptide) FQTHLPTPRGPMHCISISQQ FQTHLPTPRGPMHCISISQQ t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c a t g c a t t g c a t c t c a a t t a g t c a g c a a a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g t a c g t a a c g t a g a g t t a a t c a g t c g t t

1800 (in frame with gp41 peptide) PGVESPQAPQQAEVCKACVS SV40 promoter SV40 promoter PGVESPQAPQQAEVCKACVS c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c g t c t c a g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g c a g a g t

1860 (in frame with gp41 peptide) ISQQP* SV40 ori SV40 promoter SV40 promoter ISQQP* a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t

1920 SV40 promoter SV40 promoter SV40 ori g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c

1980 SV40 promoter SV40 promoter SV40 ori c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a

Acc65IKpnI 2040 1 5 MTEYKPT SV40 promoter PuroR T A C T G G C T C A T G T T C G G G T G Puro-R MTEYKPT t t t g c a a a a a g c t t t c t a g a g g t a c c a c c g g a g c t t a c c a t g a c c g a g t a c a a g c c c a c g a a a c g t t t t t c g a a a g a t c t c c a t g g t g g c c t c g a a t g g t a c t g g c t c a t g t t c g g g t g c

BsiWITth111IPflFI 2100 10 15 20 25 VRLATRDDVPRAVRTLAAAF PuroR VRLATRDDVPRAVRTLAAAF g t g c g c c t c g c c a c c c g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c c a c g c g g a g c g g t g g g c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g

BstEIIBspEI*RsrII 2160 30 35 40 45 ADYPATRHTVDPDRHIERVT PuroR ADYPATRHTVDPDRHIERVT g c c g a c t a c c c c g c c a c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c c g g c t g a t g g g g c g g t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g

2220 50 55 60 65 ELQELFLTRVGLDIGKVWVA PuroR ELQELFLTRVGLDIGKVWVA g a g c t g c a a g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g c t c g a c g t t c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c

2280 70 75 80 85 DDGAAVAVWTTPESVEAGAV PuroR DDGAAVAVWTTPESVEAGAV g a c g a c g g c g c c g c c g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g c t g c t g c c g c g g c g g c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c

2340 90 95 100 105 FAEIGPRMAELSGSRLAAQQ PuroR FAEIGPRMAELSGSRLAAQQ t t c g c c g a g a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a a a g c g g c t c t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t

MscI* 2400 110 115 120 125 QMEGLLAPHRPKEPAWFLAT PuroR QMEGLLAPHRPKEPAWFLAT c a g a t g g a a g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c t a c c t t c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g

2460 130 135 140 145 VGVSPDHQGKGLGSAVVLPG PuroR VGVSPDHQGKGLGSAVVLPG g t c g g c g t c t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a c a g c c g c a g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t

2520 150 155 160 165 VEAAERAGVPAFLETSAPRN PuroR G C A A C Puro-F VEAAERAGVPAFLETSAPRN g t g g a g g c g g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c a c c t c c g c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g

2580 170 175 180 185 LPFYERLGFTVTADVEVPEG PuroR C T C C C C T T C T A C G A G C Puro-F LPFYERLGFTVTADVEVPEG c t c c c c t t c t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a g a g g g g a a g a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t

HpaI 2640 190 195 200 PRTWCMTRKPGAS* PuroR PRTWCMTRKPGAS* c c g c g c a c c t g g t g c a t g a c c c g c a a g c c c g g t g c c a g t t a a c g a a t t c t a g a t c t t g a g g g c g c g t g g a c c a c g t a c t g g g c g t t c g g g c c a c g g t c a a t t g c t t a a g a t c t a g a a c t c

2700 cPPT/CTS a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g

2760 cPPT/CTS a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t

2820 cPPT/CTS a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a c t t t g g c g t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t g a a a c c g c

SbfIPspXI 2880 c c g g c t c g a g g c c t g c a g g t g c a a a g a t g g a t a a a g t t t t a a a c a g a g a g g a a t c t t t g c g g c c g a g c t c c g g a c g t c c a c g t t t c t a c c t a t t t c a a a a t t t g t c t c t c c t t a g a a a c g

2940 EF-1 α promoter a g c t a a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t g g c t c c g g t g c c c g t c a g t g g t c g a t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a c c g a g g c c a c g g g c a g t c a c c

AgeI 3000 EF-1 α promoter g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t t g a a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a a c t t g g

3060 EF-1 α promoter g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g c t c c g c c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c g a g g c g

3120 EF-1 α promoter c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g t t c t t g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g c a a g a a

3180 EF-1 α promoter EF-1 α intron A t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g c g g g c c t a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c g c c c g g a

3240 EF-1 α promoter EF-1 α intron A *KWRAATR g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c t g g c t g c a g t a c c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g a c c g a c g t c a t g

3300 EF-1 α promoter EF-1 α intron A SEQDRAEPQFHTPSNSAKRK g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a g g c c t t g c g c t c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c t c c g g a a c g c g a

3360 EF-1 α promoter EF-1 α intron A LLGKAEHKLQPRAQASPGGR t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t g g g g c c g c c g c a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g a c c c c g g c g g c g

3420 EF-1 α promoter EF-1 α intron A AFRTAGERRDRQKRYTELWK g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t a g c c a t t c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a t c g g t a a

3480 EF-1 α promoter EF-1 α intron A FNKIVQQSAKKRALYDQLHP t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t a a a t g c g a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a t t t a c g c

PspOMIApaI 3540 EF-1 α promoter EF-1 α intron A GLDACQYKPKQPRPRRRPGH g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g g g c c c g t c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c c c g g g c a

3600 EF-1 α promoter EF-1 α intron A TGACMNPSAPGALAAVSFRV g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g a a t c g g a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c t t a g c c t

FseI 3660 EF-1 α promoter EF-1 α intron A PTTELQGAQEPAQGRAATYR c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c g t g t a t c g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g c a c a t a g

3720 EF-1 α promoter EF-1 α intron A GARPPLAPGTPVLQTLPFIA g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a a a g a t g g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t t t c t a c c

3780 EF-1 α promoter EF-1 α intron A AERGQQLSSLISSAASPLAP c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c t c g g g a g a g c g g g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g a g c c c t c t c g c c

3840 EF-1 α promoter EF-1 α intron A PHTVWVFSFPRETRLRRKM g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c g c t t c a t g t c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g c a g c g a a g t a c a

3900 EF-1 α promoter EF-1 α intron A g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g a g c t t t t g g a g t c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c t c g a a a a c c t c a

3960 EF-1 α promoter EF-1 α intron A a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c t g a g t g g t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g a c t c a c c

4020 EF-1 α promoter EF-1 α intron A g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t t g c c c t t c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a a c g g g a a

4080 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t t t t t c t t a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a a a a a g a a

BclI*NheIBmtIBspDIClaI 4140 EF-1 α promoter attB1 EF-1 α intron A c c a t t t c a g g t g t c g t g a g g c t a g c a t c g a t t g a t c a a c a a g t t t g t a c a a a a a a g t t g g g g t a a a g t c c a c a g c a c t c c g a t c g t a g c t a a c t a g t t g t t c a a a c a t g t t t t t t c a a c c

4200 attB1 MLSPANGEQLHLVNYVEDY c a c c a t g t t g a g t c c t g c c a a c g g g g a g c a g c t c c a c c t g g t g a a c t a t g t g g a g g a c t a g t g g t a c a a c t c a g g a c g g t t g c c c c t c g t c g a g g t g g a c c a c t t g a t a c a c c t c c t g a t

4260 LDSIESLPFDLQRNVSLMRE c c t g g a c t c c a t c g a g t c c c t g c c t t t c g a c t t g c a g a g a a a t g t c t c g c t g a t g c g g g a g g a c c t g a g g t a g c t c a g g g a c g g a a a g c t g a a c g t c t c t t t a c a g a g c g a c t a c g c c c t

4320 IDAKYQEILKELDECYERFS *SRKL g a t c g a c g c g a a a t a c c a a g a g a t c c t g a a g g a g c t a g a c g a g t g c t a c g a g c g c t t c a g c t a g c t g c g c t t t a t g g t t c t c t a g g a c t t c c t c g a t c t g c t c a c g a t g c t c g c g a a g t c

4380 RETDGAQKRRMLHCVQRALI RSVSPACFRRISCQTCRASI t c g c g a g a c a g a c g g g g c g c a g a a g c g g c g g a t g c t g c a c t g t g t g c a g c g c g c g c t g a t a g c g c t c t g t c t g c c c c g c g t c t t c g c c g c c t a c g a c g t g a c a c a c g t c g c g c g c g a c t a

BsaBI* 4440 RSQELGDEKIQIVSQMVELV RLWSSPSSFIWITLWITSST c c g c a g c c a g g a g c t g g g c g a c g a g a a g a t c c a g a t c g t g a g c c a g a t g g t g g a g c t g g t g g c g t c g g t c c t c g a c c c g c t g c t c t t c t a g g t c t a g c a c t c g g t c t a c c a c c t c g a c c a

PmlI 4500 ENRTRQVDSHVELFEAQQEL SFRVRCTSLWTSSNSACCSS g g a g a a c c g c a c g c g g c a g g t g g a c a g c c a c g t g g a g c t g t t c g a g g c g c a g c a g g a g c t c c t c t t g g c g t g c g c c g t c c a c c t g t c g g t g c a c c t c g a c a a g c t c c g c g t c g t c c t c g a

4560 GDTAGNSGKAGADRPKGEAA PSVAPLLPLAPASLGLPSAA g g g c g a c a c a g c g g g c a a c a g c g g c a a g g c t g g c g c g g a c a g g c c c a a a g g c g a g g c g g c c c c g c t g t g t c g c c c g t t g t c g c c g t t c c g a c c g c g c c t g t c c g g g t t t c c g c t c c g c c g

4620 AQADKPNSKRSRRQRNNENR ACASLGLLLRERRCRLLSFR a g c g c a g g c t g a c a a g c c c a a c a g c a a g c g c t c a c g g c g g c a g c g c a a c a a c g a g a a c c g t c g c g t c c g a c t g t t c g g g t t g t c g t t c g c g a g t g c c g c c g t c g c g t t g t t g c t c t t g g c

4680 ENASSNHDHDDGASGTPKEK SFADLLWSWSSPAEPVGLSF t g a g a a c g c g t c c a g c a a c c a c g a c c a c g a c g a c g g c g c c t c g g g c a c a c c c a a g g a g a a a c t c t t g c g c a g g t c g t t g g t g c t g g t g c t g c t g c c g c g g a g c c c g t g t g g g t t c c t c t t

4740 KAKTSKKKKRSKAKAEREAS FALVELFFFRELALASRSAD g a a g g c c a a g a c c t c c a a g a a g a a g a a g c g c t c c a a g g c c a a g g c g g a g c g a g a g g c g t c c t t c c g g t t c t g g a g g t t c t t c t t c t t c g c g a g g t t c c g g t t c c g c c t c g c t c t c c g c a g

XcmI 4800 PADLPIDPNEPTYCLCNQVS GASRGM c c c t g c c g a c c t c c c c a t c g a c c c c a a c g a a c c c a c g t a c t g t c t g t g c a a c c a g g t c t c g g g a c g g c t g g a g g g g t a g c t g g g g t t g c t t g g g t g c a t g a c a g a c a c g t t g g t c c a g a g

4860 YGEMIGCDNDECPIEWFHFS c t a t g g g g a g a t g a t c g g c t g c g a c a a c g a c g a g t g c c c c a t c g a g t g g t t c c a c t t c t c g a t a c c c c t c t a c t a g c c g a c g c t g t t g c t g c t c a c g g g g t a g c t c a c c a a g g t g a a g a g

4920 CVGLNHKPKGKWYCPKCRGE g t g c g t g g g g c t c a a t c a t a a a c c c a a g g g c a a g t g g t a c t g t c c c a a g t g c c g g g g g g a c a c g c a c c c c g a g t t a g t a t t t g g g t t c c c g t t c a c c a t g a c a g g g t t c a c g g c c c c c c t

4980 NEKTMDKALEKSKKERAYNR g a a c g a g a a g a c c a t g g a c a a a g c c c t g g a g a a a t c c a a a a a a g a g a g g g c t t a c a a c a g c t t g c t c t t c t g g t a c c t g t t t c g g g a c c t c t t t a g g t t t t t t c t c t c c c g a a t g t t g t c

EcoRV 5040 1 5 GKPIPNPLL attB2 V5 tag LPTFLYKVVDIGKPIPNPLL g t t g c c a a c t t t c t t g t a c a a a g t g g t t g a t a t c g g t a a g c c t a t c c c t a a c c c t c t c c t c a a c g g t t g a a a g a a c a t g t t t c a c c a a c t a t a g c c a t t c g g a t a g g g a t t g g g a g a g g a

SpeI 5100 10 GLDST * V5 tag GLDST* c g g t c t c g a t t c t a c g t a g t a a t g a a c t a g t c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c c a g a g c t a a g a t g c a t c a t t a c t t g a t c a g g c a t t g a a c t t t c a t a a a g c t a a a g a a c

5160 g c t t t a t a t a t c t t g t g g a a a g g a c g a g t a t c t a a c t c t t t t t t g a a t t c g a c g c g t t a a c g a a a t a t a t a g a a c a c c t t t c c t g c t c a t a g a t t g a g a a a a a a c t t a a g c t g c g c a a t t

5220 WPRE A WPRE-R g t c g a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t c a g c t g t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a

5280 WPRE C A A C G A G G A A A A T G C G A T A C WPRE-R MPLYHAIA MLLL g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a

5340 WPRE SRMAFIFSSLYKSWLLSLYE PVWLSFSPPCINPGCCLFMR t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c

5400 WPRE ELWPVVRQRGVVCTVFADAT SCGPLSGNVAWCALCLLTQP g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g

5460 WPRE PTGWGIATTCQLLSGTFAFP PLVGALPPPVSSFPGLSLSP c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g

5520 WPRE LPIATAELIAACLARCWTGA SLLPRRNSSPPALPAAGQGL c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a

5580 WPRE (in frame with Factor Xa site) *RGKRP RLLGTDNSVVLSGKSSSFPW GCWALTIPWCCRGNHRPFLG c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c

5640 WPRE (in frame with Factor Xa site) QEGTNGGPNQAPRGEAVDR R Factor Xa site LLACVATWILRGTSFCYVPS CSPVLPPGFCAGRPSATSLR c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c

5700 WPRE 1 GEI Factor Xa site ALNPADLPSRGLLPALRPLP PSIQRTFLPAACCRLCGLFR g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c

5760 WPRE RLRLRPQTSRISLWAASPRR VFAFALRRVGSPFGPPPRVD c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c g t c g a g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g c a g c t

5820 L* FKTNDLQGSCRS* c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c

5880 3' LTR ( Δ U3) g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t c t g c t t t t t g c t t g t a c t g c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a g a c g a a a a a c g a a c a t g a c

5940 3' LTR ( Δ U3) g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g

6000 3' LTR ( Δ U3) t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a

6060 3' LTR ( Δ U3) g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t

6120 g t a c g t a t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t a a c t t g c a a a g a a a t g a a c a t g c a t a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a t t g a a c g t t t c t t t a c t t

6180 SV40 poly(A) signal C G T T A T SV40pA-R t a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a a t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t

6240 SV40 poly(A) signal G T G G T T T G T C C A EBV-rev C G T A G T G T T T A A A G SV40pA-R g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t

6300 SV40 poly(A) signal SV40 ori A A C T C A T C EBV-rev a a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t a g c t a t c c c g c c c c t a a c t c c g c c c a t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a t c g a t a g g g c g g g g a t t g a g g c g g g t a

6360 SV40 ori c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a

6420 SV40 ori t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c

6480 SV40 ori T7 promoter G G G A T A T C A C T C A G C A T A A T T7 c t t t t t t g g a g g c c t a g g g a c g t a c c c a a t t c g c c c t a t a g t g a g t c g t a t t a c g c g c g c g a a a a a a c c t c c g g a t c c c t g c a t g g g t t a a g c g g g a t a t c a c t c a g c a t a a t g c g c g c g

6540 M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a

6600 c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a

6660 f1 ori c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g g a c g c g c c c t g t a g c g g c g c a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c c t g c g c g g g a c a t c g c c g c g t

6720 f1 ori t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t

6780 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a

6840 f1 ori c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g

6900 f1 ori c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a

6960 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t

7020 f1 ori a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c

7080 f1 ori g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t

7140 f1 ori AmpR promoter t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a

7200 AmpR promoter t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g

7260 1 5 10 signal sequence MSIQHFRVALI AmpR promoter AmpR MSIQHFRVALI t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g

7320 15 20 signal sequence PFFAAFCLPVFA 25 30 HPETLVKV AmpR PFFAAFCLPVFAHPETLVKV c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t

7380 35 40 45 50 KDAEDQLGARVGYIELDLNS AmpR KDAEDQLGARVGYIELDLNS a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c

7440 55 60 65 70 GKILESFRPEERFPMMSTFK AmpR GKILESFRPEERFPMMSTFK g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c

7500 75 80 85 90 VLLCGAVLSRIDAGQEQLGR AmpR C G A T A C A C C G C G C C A T A A T A Amp-R VLLCGAVLSRIDAGQEQLGR t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g

ScaI 7560 95 100 105 110 RIHYSQNDLVEYSPVTEKHL AmpR RIHYSQNDLVEYSPVTEKHL g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t

7620 115 120 125 130 TDGMTVRELCSAAITMSDNT AmpR TDGMTVRELCSAAITMSDNT c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c

7680 135 140 145 150 AANLLLTTIGGPKELTAFLH AmpR AANLLLTTIGGPKELTAFLH c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t

7740 155 160 165 170 NMGDHVTRLDRWEPELNEAI AmpR NMGDHVTRLDRWEPELNEAI a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g

7800 175 180 185 190 PNDERDTTMPVAMATTLRKL AmpR PNDERDTTMPVAMATTLRKL c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a

7860 195 200 205 210 LTGELLTLASRQQLIDWMEA AmpR LTGELLTLASRQQLIDWMEA t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c

7920 215 220 225 230 DKVAGPLLRSALPAGWFIAD AmpR DKVAGPLLRSALPAGWFIAD a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t

7980 235 240 245 250 KSGAGERGSRGIIAALGPDG AmpR KSGAGERGSRGIIAALGPDG a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t

AhdI 8040 255 260 265 270 KPSRIVVIYTTGSQATMDER AmpR KPSRIVVIYTTGSQATMDER a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t

8100 275 280 285 NRQIAEIGASLIKHW* AmpR NRQIAEIGASLIKHW* a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c

8160 t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c

8220 t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a

8280 ori g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c

8340 ori t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g

8400 ori a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t

8460 ori c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t

8520 ori c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g

8580 ori t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c

8640 ori g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g

8700 ori a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c

8760 ori G G G A A A C G C C T G G T pBR322ori-F t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a

8820 ori A T C T T T pBR322ori-F a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a

8880 ori c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c

8940 c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t

9000 A L4440 a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t

9060 G C G A G T C A G T G A G C G A G L4440 g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g

9120 g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c

9180 -35 CAP binding site lac promoter a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t

9240 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A M13 Reverse t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t

9300 M13 rev T3 promoter G C T A T G A C M13 Reverse G C A A T T A A C C C T C A C T A A A G G T3 g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c

9310 a g c t g c a a g c t c g a c g t t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.