Addgene pCRISPRi Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pCRISPRi Sequencing Result


Map View

PspXI (10,926) SacII (10,922) StuI (10,822) AvrII (9916) ApaI (9913) PspOMI (9909) BspDI - ClaI (9416) XbaI (9310) PacI (7767) PpuMI (6911) AscI - BssHII (6362) SmaI (6340) TspMI - XmaI (6338) BamHI (5517) pGEX 3' (52 .. 74) pBRforEco (112 .. 130) ZraI (169) AatII (171) Amp-R (521 .. 540) AhdI (1090) BseYI (1674) PspFI (1678) pBR322ori-F (1823 .. 1842) SalI (2121) BstZ17I (2354) BmgBI (2359) BsgI (2762) BstAPI (2920) NheI (3238) BmtI (3242) BstBI (3477) BsmI (3743) PmlI (4432) NruI (4470) MluI (4711) pCRISPRi 10,985 bp

Sequence View

5ʹ 3ʹ 60 C C G G G A G C T pGEX 3' g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a

120 G C A T G T G T C A G A G G pGEX 3' C G G A G C A C T pBRforEco g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c t

ZraIAatII 180 A T G C G G A T A A pBRforEco t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t

240 AmpR promoter c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t

300 AmpR promoter t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t

360 1 5 10 15 signal sequence MSIQHFRVALIPFFAAFCL AmpR promoter AmpR MSIQHFRVALIPFFAAFCL g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g

420 20 signal sequence PVFA 25 30 35 HPETLVKVKDAEDQLG AmpR PVFAHPETLVKVKDAEDQLG c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c

480 40 45 50 55 ARVGYIELDLNSGKILESFR AmpR ARVGYIELDLNSGKILESFR c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g

540 60 65 70 75 PEERFPMMSTFKVLLCGAVL AmpR C G A T A C A C C G C G C C A T A A T A Amp-R PEERFPMMSTFKVLLCGAVL c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a

600 80 85 90 95 SRIDAGQEQLGRRIHYSQND AmpR SRIDAGQEQLGRRIHYSQND c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a

660 100 105 110 115 LVEYSPVTEKHLTDGMTVRE AmpR LVEYSPVTEKHLTDGMTVRE t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a

720 120 125 130 135 LCSAAITMSDNTAANLLLTT AmpR LCSAAITMSDNTAANLLLTT t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t

780 140 145 150 155 IGGPKELTAFLHNMGDHVTR AmpR IGGPKELTAFLHNMGDHVTR t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g

840 160 165 170 175 LDRWEPELNEAIPNDERDTT AmpR LDRWEPELNEAIPNDERDTT t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t

900 180 185 190 195 MPVAMATTLRKLLTGELLTL AmpR MPVAMATTLRKLLTGELLTL t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c

960 200 205 210 215 ASRQQLIDWMEADKVAGPLL AmpR ASRQQLIDWMEADKVAGPLL c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g

1020 220 225 230 235 RSALPAGWFIADKSGAGERG AmpR RSALPAGWFIADKSGAGERG g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g t t c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c a a

1080 240 245 250 255 SRGIIAALGPDGKPSRIVVI AmpR SRGIIAALGPDGKPSRIVVI c a c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t g t g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a

AhdI 1140 260 265 270 275 YTTGSQATMDERNRQIAEIG AmpR YTTGSQATMDERNRQIAEIG a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c

1200 280 285 ASLIKHW* AmpR ASLIKHW* c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c

1260 a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t

1320 t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t

1380 ori t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t

1440 ori a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t

1500 ori a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a

1560 ori t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a

1620 ori t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a

BseYIPspFI 1680 ori a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a

1740 ori t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t

1800 ori c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c

1860 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g

1920 ori g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t

1980 ori a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t

2040 t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c

2100 c t g g c g g c c g c t g c a t g c c t g c a g g g c t g g c g g c c g c t g c a t g a t g a c a a a a a g a g c a a a g a c c g c c g g c g a c g t a c g g a c g t c c c g a c c g c c g g c g a c g t a c t a c t g t t t t t c t c g t t t

SalI 2160 1 5 MDKKYSI dCas9 MDKKYSI a t t t t g a t a a a a t a g t a t t a g t c g a c a a a g g a g g c a a a a a t g g a t a a g a a a t a c t c a a t a t a a a a c t a t t t t a t c a t a a t c a g c t g t t t c c t c c g t t t t t a c c t a t t c t t t a t g a g t t a t

2220 GL 10 A 15 20 25 IGTNSVGWAVITDEYKV dCas9 GLAIGTNSVGWAVITDEYKV g g c t t a g c t a t c g g c a c a a a t a g c g t c g g a t g g g c g g t g a t c a c t g a t g a a t a t a a g g t t c c g a a t c g a t a g c c g t g t t t a t c g c a g c c t a c c c g c c a c t a g t g a c t a c t t a t a t t c c a a

2280 30 35 40 45 PSKKFKVLGNTDRHSIKKNL dCas9 PSKKFKVLGNTDRHSIKKNL c c g t c t a a a a a g t t c a a g g t t c t g g g a a a t a c a g a c c g c c a c a g t a t c a a a a a a a a t c t t g g c a g a t t t t t c a a g t t c c a a g a c c c t t t a t g t c t g g c g g t g t c a t a g t t t t t t t t a g a a

2340 50 55 60 65 IGALLFDSGETAEATRLKRT dCas9 IGALLFDSGETAEATRLKRT a t a g g g g c t c t t t t a t t t g a c a g t g g a g a g a c a g c g g a a g c g a c t c g t c t c a a a c g g a c a t a t c c c c g a g a a a a t a a a c t g t c a c c t c t c t g t c g c c t t c g c t g a g c a g a g t t t g c c t g t

BstZ17IBmgBI 2400 70 75 80 85 ARRRYTRRKNRICYLQEIFS dCas9 ARRRYTRRKNRICYLQEIFS g c t c g t a g a a g g t a t a c a c g t c g g a a g a a t c g t a t t t g t t a t c t a c a g g a g a t t t t t t c a c g a g c a t c t t c c a t a t g t g c a g c c t t c t t a g c a t a a a c a a t a g a t g t c c t c t a a a a a a g t

2460 90 95 100 105 NEMAKVDDSFFHRLEESFLV dCas9 NEMAKVDDSFFHRLEESFLV a a t g a g a t g g c g a a a g t a g a t g a t a g t t t c t t t c a t c g a c t t g a a g a g t c t t t t t t g g t g t t a c t c t a c c g c t t t c a t c t a c t a t c a a a g a a a g t a g c t g a a c t t c t c a g a a a a a a c c a c

2520 110 115 120 125 EEDKKHERHPIFGNIVDEVA dCas9 EEDKKHERHPIFGNIVDEVA g a a g a a g a c a a g a a g c a t g a a c g t c a t c c t a t t t t t g g a a a t a t a g t a g a t g a a g t t g c t c t t c t t c t g t t c t t c g t a c t t g c a g t a g g a t a a a a a c c t t t a t a t c a t c t a c t t c a a c g a

2580 130 135 140 145 YHEKYPTIYHLRKKLVDSTD dCas9 YHEKYPTIYHLRKKLVDSTD t a t c a t g a g a a a t a t c c a a c t a t c t a t c a t c t g c g a a a a a a a t t g g t a g a t t c t a c t g a t a t a g t a c t c t t t a t a g g t t g a t a g a t a g t a g a c g c t t t t t t t a a c c a t c t a a g a t g a c t a

2640 150 155 160 165 KADLRLIYLALAHMIKFRGH dCas9 KADLRLIYLALAHMIKFRGH a a a g c g g a t t t g c g c t t a a t c t a t t t g g c c t t a g c g c a t a t g a t t a a g t t t c g t g g t c a t t t t c g c c t a a a c g c g a a t t a g a t a a a c c g g a a t c g c g t a t a c t a a t t c a a a g c a c c a g t a

2700 170 175 180 185 FLIEGDLNPDNSDVDKLFIQ dCas9 FLIEGDLNPDNSDVDKLFIQ t t t t t g a t t g a g g g a g a t t t a a a t c c t g a t a a t a g t g a t g t g g a c a a a c t a t t t a t c c a g a a a a a c t a a c t c c c t c t a a a t t t a g g a c t a t t a t c a c t a c a c c t g t t t g a t a a a t a g g t c

2760 190 195 200 205 LVQTYNQLFEENPINASGVD dCas9 LVQTYNQLFEENPINASGVD t t g g t a c a a a c c t a c a a t c a a t t a t t t g a a g a a a a c c c t a t t a a c g c a a g t g g a g t a g a t a a c c a t g t t t g g a t g t t a g t t a a t a a a c t t c t t t t g g g a t a a t t g c g t t c a c c t c a t c t a

BsgI 2820 210 215 220 225 AKAILSARLSKSRRLENLIA dCas9 AKAILSARLSKSRRLENLIA g c t a a a g c g a t t c t t t c t g c a c g a t t g a g t a a a t c a a g a c g a t t a g a a a a t c t c a t t g c t c g a t t t c g c t a a g a a a g a c g t g c t a a c t c a t t t a g t t c t g c t a a t c t t t t a g a g t a a c g a

2880 230 235 240 245 QLPGEKKNGLFGNLIALSLG dCas9 QLPGEKKNGLFGNLIALSLG c a g c t c c c c g g t g a g a a g a a a a a t g g c t t a t t t g g g a a t c t c a t t g c t t t g t c a t t g g g t g t c g a g g g g c c a c t c t t c t t t t t a c c g a a t a a a c c c t t a g a g t a a c g a a a c a g t a a c c c a

BstAPI 2940 250 255 260 265 LTPNFKSNFDLAEDAKLQLS dCas9 LTPNFKSNFDLAEDAKLQLS t t g a c c c c t a a t t t t a a a t c a a a t t t t g a t t t g g c a g a a g a t g c t a a a t t a c a g c t t t c a a a c t g g g g a t t a a a a t t t a g t t t a a a a c t a a a c c g t c t t c t a c g a t t t a a t g t c g a a a g t

3000 270 275 280 285 KDTYDDDLDNLLAQIGDQYA dCas9 KDTYDDDLDNLLAQIGDQYA a a a g a t a c t t a c g a t g a t g a t t t a g a t a a t t t a t t g g c g c a a a t t g g a g a t c a a t a t g c t t t t c t a t g a a t g c t a c t a c t a a a t c t a t t a a a t a a c c g c g t t t a a c c t c t a g t t a t a c g a

3060 290 295 300 305 DLFLAAKNLSDAILLSDILR dCas9 DLFLAAKNLSDAILLSDILR g a t t t g t t t t t g g c a g c t a a g a a t t t a t c a g a t g c t a t t t t a c t t t c a g a t a t c c t a a g a c t a a a c a a a a a c c g t c g a t t c t t a a a t a g t c t a c g a t a a a a t g a a a g t c t a t a g g a t t c t

3120 310 315 320 325 VNTEITKAPLSASMIKRYDE dCas9 VNTEITKAPLSASMIKRYDE g t a a a t a c t g a a a t a a c t a a g g c t c c c c t a t c a g c t t c a a t g a t t a a a c g c t a c g a t g a a c a t t t a t g a c t t t a t t g a t t c c g a g g g g a t a g t c g a a g t t a c t a a t t t g c g a t g c t a c t t

3180 330 335 340 345 HHQDLTLLKALVRQQLPEKY dCas9 HHQDLTLLKALVRQQLPEKY c a t c a t c a a g a c t t g a c t c t t t t a a a a g c t t t a g t t c g a c a a c a a c t t c c a g a a a a g t a t g t a g t a g t t c t g a a c t g a g a a a a t t t t c g a a a t c a a g c t g t t g t t g a a g g t c t t t t c a t a

NheI 3240 350 355 360 365 KEIFFDQSKNGYAGYIDGGA dCas9 KEIFFDQSKNGYAGYIDGGA a a a g a a a t c t t t t t t g a t c a a t c a a a a a a c g g a t a t g c a g g t t a t a t t g a t g g g g g a g c t t t t c t t t a g a a a a a a c t a g t t a g t t t t t t g c c t a t a c g t c c a a t a t a a c t a c c c c c t c g a

BmtI 3300 370 375 380 385 SQEEFYKFIKPILEKMDGTE dCas9 SQEEFYKFIKPILEKMDGTE a g c c a a g a a g a a t t t t a t a a a t t t a t c a a a c c a a t t t t a g a a a a a a t g g a t g g t a c t g a g t c g g t t c t t c t t a a a a t a t t t a a a t a g t t t g g t t a a a a t c t t t t t t a c c t a c c a t g a c t c

3360 390 395 400 405 ELLVKLNREDLLRKQRTFDN dCas9 ELLVKLNREDLLRKQRTFDN g a a t t a t t g g t g a a a c t a a a t c g t g a a g a t t t g c t g c g c a a g c a a c g g a c c t t t g a c a a c c t t a a t a a c c a c t t t g a t t t a g c a c t t c t a a a c g a c g c g t t c g t t g c c t g g a a a c t g t t g

3420 410 415 420 425 GSIPHQIHLGELHAILRRQE dCas9 GSIPHQIHLGELHAILRRQE g g c t c t a t t c c c c a t c a a a t t c a c t t g g g t g a g c t g c a t g c t a t t t t g a g a a g a c a a g a a c c g a g a t a a g g g g t a g t t t a a g t g a a c c c a c t c g a c g t a c g a t a a a a c t c t t c t g t t c t t

BstBI 3480 430 435 440 445 DFYPFLKDNREKIEKILTFR dCas9 DFYPFLKDNREKIEKILTFR g a c t t t t a t c c a t t t t t a a a a g a c a a t c g t g a g a a g a t t g a a a a a a t c t t g a c t t t t c g a c t g a a a a t a g g t a a a a a t t t t c t g t t a g c a c t c t t c t a a c t t t t t t a g a a c t g a a a a g c t

3540 450 455 460 465 IPYYVGPLARGNSRFAWMTR dCas9 IPYYVGPLARGNSRFAWMTR a t t c c t t a t t a t g t t g g t c c a t t g g c g c g t g g c a a t a g t c g t t t t g c a t g g a t g a c t c g g t a a g g a a t a a t a c a a c c a g g t a a c c g c g c a c c g t t a t c a g c a a a a c g t a c c t a c t g a g c c

3600 470 475 480 485 KSEETITPWNFEEVVDKGAS dCas9 KSEETITPWNFEEVVDKGAS a a g t c t g a a g a a a c a a t t a c c c c a t g g a a t t t t g a a g a a g t t g t c g a t a a a g g t g c t t c a t t c a g a c t t c t t t g t t a a t g g g g t a c c t t a a a a c t t c t t c a a c a g c t a t t t c c a c g a a g t

3660 490 495 500 505 AQSFIERMTNFDKNLPNEKV dCas9 AQSFIERMTNFDKNLPNEKV g c t c a a t c a t t t a t t g a a c g c a t g a c a a a c t t t g a t a a a a a t c t t c c a a a t g a a a a a g t a c g a g t t a g t a a a t a a c t t g c g t a c t g t t t g a a a c t a t t t t t a g a a g g t t t a c t t t t t c a t

3720 510 515 520 525 LPKHSLLYEYFTVYNELTKV dCas9 LPKHSLLYEYFTVYNELTKV c t a c c a a a a c a t a g t t t g c t t t a t g a g t a t t t t a c g g t t t a t a a c g a a t t g a c a a a g g t c g a t g g t t t t g t a t c a a a c g a a a t a c t c a t a a a a t g c c a a a t a t t g c t t a a c t g t t t c c a g

BsmI 3780 530 535 540 545 KYVTEGMRKPAFLSGEQKKA dCas9 KYVTEGMRKPAFLSGEQKKA a a a t a t g t t a c t g a a g g a a t g c g a a a a c c a g c a t t t c t t t c a g g t g a a c a g a a g a a a g c c t t t a t a c a a t g a c t t c c t t a c g c t t t t g g t c g t a a a g a a a g t c c a c t t g t c t t c t t t c g g

3840 550 555 560 565 IVDLLFKTNRKVTVKQLKED dCas9 IVDLLFKTNRKVTVKQLKED a t t g t t g a t t t a c t c t t c a a a a c a a a t c g a a a a g t a a c c g t t a a g c a a t t a a a a g a a g a t t a a c a a c t a a a t g a g a a g t t t t g t t t a g c t t t t c a t t g g c a a t t c g t t a a t t t t c t t c t a

3900 570 575 580 585 YFKKIECFDSVEISGVEDRF dCas9 YFKKIECFDSVEISGVEDRF t a t t t c a a a a a a a t a g a a t g t t t t g a t a g t g t t g a a a t t t c a g g a g t t g a a g a t a g a t t t a t a a a g t t t t t t t a t c t t a c a a a a c t a t c a c a a c t t t a a a g t c c t c a a c t t c t a t c t a a a

3960 590 595 600 605 NASLGTYHDLLKIIKDKDFL dCas9 NASLGTYHDLLKIIKDKDFL a a t g c t t c a t t a g g t a c c t a c c a t g a t t t g c t a a a a a t t a t t a a a g a t a a a g a t t t t t t g t t a c g a a g t a a t c c a t g g a t g g t a c t a a a c g a t t t t t a a t a a t t t c t a t t t c t a a a a a a c

4020 610 615 620 625 DNEENEDILEDIVLTLTLFE dCas9 DNEENEDILEDIVLTLTLFE g a t a a t g a a g a a a a t g a a g a t a t c t t a g a g g a t a t t g t t t t a a c a t t g a c c t t a t t t g a a c t a t t a c t t c t t t t a c t t c t a t a g a a t c t c c t a t a a c a a a a t t g t a a c t g g a a t a a a c t t

4080 630 635 640 645 DREMIEERLKTYAHLFDDKV dCas9 DREMIEERLKTYAHLFDDKV g a t a g g g a g a t g a t t g a g g a a a g a c t t a a a a c a t a t g c t c a c c t c t t t g a t g a t a a g g t g c t a t c c c t c t a c t a a c t c c t t t c t g a a t t t t g t a t a c g a g t g g a g a a a c t a c t a t t c c a c

4140 650 655 660 665 MKQLKRRRYTGWGRLSRKLI dCas9 MKQLKRRRYTGWGRLSRKLI a t g a a a c a g c t t a a a c g t c g c c g t t a t a c t g g t t g g g g a c g t t t g t c t c g a a a a t t g a t t t a c t t t g t c g a a t t t g c a g c g g c a a t a t g a c c a a c c c c t g c a a a c a g a g c t t t t a a c t a a

4200 670 675 680 685 NGIRDKQSGKTILDFLKSDG dCas9 NGIRDKQSGKTILDFLKSDG a a t g g t a t t a g g g a t a a g c a a t c t g g c a a a a c a a t a t t a g a t t t t t t g a a a t c a g a t g g t t t a c c a t a a t c c c t a t t c g t t a g a c c g t t t t g t t a t a a t c t a a a a a a c t t t a g t c t a c c a

4260 690 695 700 705 FANRNFMQLIHDDSLTFKED dCas9 FANRNFMQLIHDDSLTFKED t t t g c c a a t c g c a a t t t t a t g c a g c t g a t c c a t g a t g a t a g t t t g a c a t t t a a a g a a g a c a a a c g g t t a g c g t t a a a a t a c g t c g a c t a g g t a c t a c t a t c a a a c t g t a a a t t t c t t c t g

4320 710 715 720 725 IQKAQVSGQGDSLHEHIANL dCas9 IQKAQVSGQGDSLHEHIANL a t t c a a a a a g c a c a a g t g t c t g g a c a a g g c g a t a g t t t a c a t g a a c a t a t t g c a a a t t t a t a a g t t t t t c g t g t t c a c a g a c c t g t t c c g c t a t c a a a t g t a c t t g t a t a a c g t t t a a a t

4380 730 735 740 745 AGSPAIKKGILQTVKVVDEL dCas9 AGSPAIKKGILQTVKVVDEL g c t g g t a g c c c t g c t a t t a a a a a a g g t a t t t t a c a g a c t g t a a a a g t t g t t g a t g a a t t g c g a c c a t c g g g a c g a t a a t t t t t t c c a t a a a a t g t c t g a c a t t t t c a a c a a c t a c t t a a c

PmlI 4440 750 755 760 765 VKVMGRHKPENIVIEMAREN dCas9 VKVMGRHKPENIVIEMAREN g t c a a a g t a a t g g g g c g g c a t a a g c c a g a a a a t a t c g t t a t t g a a a t g g c a c g t g a a a a t c a g t t t c a t t a c c c c g c c g t a t t c g g t c t t t t a t a g c a a t a a c t t t a c c g t g c a c t t t t a

NruI 4500 770 775 780 785 QTTQKGQKNSRERMKRIEEG dCas9 QTTQKGQKNSRERMKRIEEG c a g a c a a c t c a a a a g g g c c a g a a a a a t t c g c g a g a g c g t a t g a a a c g a a t c g a a g a a g g t g t c t g t t g a g t t t t c c c g g t c t t t t t a a g c g c t c t c g c a t a c t t t g c t t a g c t t c t t c c a

4560 790 795 800 805 IKELGSQILKEHPVENTQLQ dCas9 IKELGSQILKEHPVENTQLQ a t c a a a g a a t t a g g a a g t c a g a t t c t t a a a g a g c a t c c t g t t g a a a a t a c t c a a t t g c a a t a g t t t c t t a a t c c t t c a g t c t a a g a a t t t c t c g t a g g a c a a c t t t t a t g a g t t a a c g t t

4620 810 815 820 825 NEKLYLYYLQNGRDMYVDQE dCas9 NEKLYLYYLQNGRDMYVDQE a a t g a a a a g c t c t a t c t c t a t t a t c t c c a a a a t g g a a g a g a c a t g t a t g t g g a c c a a g a a t t a c t t t t c g a g a t a g a g a t a a t a g a g g t t t t a c c t t c t c t g t a c a t a c a c c t g g t t c t t

4680 830 835 LDINRLSDYDVD 840 A 845 IVPQSFL dCas9 LDINRLSDYDVDAIVPQSFL t t a g a t a t t a a t c g t t t a a g t g a t t a t g a t g t c g a t g c c a t t g t t c c a c a a a g t t t c c t t a a t c t a t a a t t a g c a a a t t c a c t a a t a c t a c a g c t a c g g t a a c a a g g t g t t t c a a a g g a a

MluI 4740 850 855 860 865 KDDSIDNKVLTRSDKNRGKS dCas9 KDDSIDNKVLTRSDKNRGKS a a a g a c g a t t c a a t a g a c a a t a a g g t c t t a a c g c g t t c t g a t a a a a a t c g t g g t a a a t c g t t t c t g c t a a g t t a t c t g t t a t t c c a g a a t t g c g c a a g a c t a t t t t t a g c a c c a t t t a g c

4800 870 875 880 885 DNVPSEEVVKKMKNYWRQLL dCas9 DNVPSEEVVKKMKNYWRQLL g a t a a c g t t c c a a g t g a a g a a g t a g t c a a a a a g a t g a a a a a c t a t t g g a g a c a a c t t c t a c t a t t g c a a g g t t c a c t t c t t c a t c a g t t t t t c t a c t t t t t g a t a a c c t c t g t t g a a g a t

4860 890 895 900 905 NAKLITQRKFDNLTKAERGG dCas9 NAKLITQRKFDNLTKAERGG a a c g c c a a g t t a a t c a c t c a a c g t a a g t t t g a t a a t t t a a c g a a a g c t g a a c g t g g a g g t t t g c g g t t c a a t t a g t g a g t t g c a t t c a a a c t a t t a a a t t g c t t t c g a c t t g c a c c t c c a

4920 910 915 920 925 LSELDKAGFIKRQLVETRQI dCas9 LSELDKAGFIKRQLVETRQI t t g a g t g a a c t t g a t a a a g c t g g t t t t a t c a a a c g c c a a t t g g t t g a a a c t c g c c a a a t c a a c t c a c t t g a a c t a t t t c g a c c a a a a t a g t t t g c g g t t a a c c a a c t t t g a g c g g t t t a g

4980 930 935 940 945 TKHVAQILDSRMNTKYDEND dCas9 TKHVAQILDSRMNTKYDEND a c t a a g c a t g t g g c a c a a a t t t t g g a t a g t c g c a t g a a t a c t a a a t a c g a t g a a a a t g a t t g a t t c g t a c a c c g t g t t t a a a a c c t a t c a g c g t a c t t a t g a t t t a t g c t a c t t t t a c t a

5040 950 955 960 965 KLIREVKVITLKSKLVSDFR dCas9 KLIREVKVITLKSKLVSDFR a a a c t t a t t c g a g a g g t t a a a g t g a t t a c c t t a a a a t c t a a a t t a g t t t c t g a c t t c c g a t t t g a a t a a g c t c t c c a a t t t c a c t a a t g g a a t t t t a g a t t t a a t c a a a g a c t g a a g g c t

5100 970 975 980 985 KDFQFYKVREINNYHHAHDA dCas9 KDFQFYKVREINNYHHAHDA a a a g a t t t c c a a t t c t a t a a a g t a c g t g a g a t t a a c a a t t a c c a t c a t g c c c a t g a t g c g t t t c t a a a g g t t a a g a t a t t t c a t g c a c t c t a a t t g t t a a t g g t a g t a c g g g t a c t a c g c

5160 990 995 1000 1005 YLNAVVGTALIKKYPKLESE dCas9 YLNAVVGTALIKKYPKLESE t a t c t a a a t g c c g t c g t t g g a a c t g c t t t g a t t a a g a a a t a t c c a a a a c t t g a a t c g g a g a t a g a t t t a c g g c a g c a a c c t t g a c g a a a c t a a t t c t t t a t a g g t t t t g a a c t t a g c c t c

5220 1010 1015 1020 1025 FVYGDYKVYDVRKMIAKSEQ dCas9 FVYGDYKVYDVRKMIAKSEQ t t t g t c t a t g g t g a t t a t a a a g t t t a t g a t g t t c g t a a a a t g a t t g c t a a g t c t g a g c a a a a a c a g a t a c c a c t a a t a t t t c a a a t a c t a c a a g c a t t t t a c t a a c g a t t c a g a c t c g t t

5280 1030 1035 1040 1045 EIGKATAKYFFYSNIMNFFK dCas9 EIGKATAKYFFYSNIMNFFK g a a a t a g g c a a a g c a a c c g c a a a a t a t t t c t t t t a c t c t a a t a t c a t g a a c t t c t t c a a a c t t t a t c c g t t t c g t t g g c g t t t t a t a a a g a a a a t g a g a t t a t a g t a c t t g a a g a a g t t t

5340 1050 1055 1060 1065 TEITLANGEIRKRPLIETNG dCas9 TEITLANGEIRKRPLIETNG a c a g a a a t t a c a c t t g c a a a t g g a g a g a t t c g c a a a c g c c c t c t a a t c g a a a c t a a t g g g t g t c t t t a a t g t g a a c g t t t a c c t c t c t a a g c g t t t g c g g g a g a t t a g c t t t g a t t a c c c

5400 1070 1075 1080 1085 ETGEIVWDKGRDFATVRKVL dCas9 ETGEIVWDKGRDFATVRKVL g a a a c t g g a g a a a t t g t c t g g g a t a a a g g g c g a g a t t t t g c c a c a g t g c g c a a a g t a t t g c t t t g a c c t c t t t a a c a g a c c c t a t t t c c c g c t c t a a a a c g g t g t c a c g c g t t t c a t a a c

5460 1090 1095 1100 1105 SMPQVNIVKKTEVQTGGFSK dCas9 SMPQVNIVKKTEVQTGGFSK t c c a t g c c c c a a g t c a a t a t t g t c a a g a a a a c a g a a g t a c a g a c a g g c g g a t t c t c c a a g a g g t a c g g g g t t c a g t t a t a a c a g t t c t t t t g t c t t c a t g t c t g t c c g c c t a a g a g g t t c

BamHI 5520 1110 1115 1120 1125 EPILPKRNSDKLIARKKDWD dCas9 EPILPKRNSDKLIARKKDWD g a g c c a a t t t t a c c a a a a a g a a a t t c g g a c a a g c t t a t t g c t c g t a a a a a a g a c t g g g a t c t c g g t t a a a a t g g t t t t t c t t t a a g c c t g t t c g a a t a a c g a g c a t t t t t t c t g a c c c t a

5580 1130 1135 1140 1145 PKKYGGFDSPTVAYSVLVVA dCas9 PKKYGGFDSPTVAYSVLVVA c c a a a a a a a t a t g g t g g t t t t g a t a g t c c a a c g g t a g c t t a t t c a g t c c t a g t g g t t g c t g g t t t t t t t a t a c c a c c a a a a c t a t c a g g t t g c c a t c g a a t a a g t c a g g a t c a c c a a c g a

5640 1150 1155 1160 1165 KVEKGKSKKLKSVKELLGIT dCas9 KVEKGKSKKLKSVKELLGIT a a g g t g g a a a a a g g g a a a t c g a a g a a g t t a a a a t c c g t t a a a g a g t t a c t a g g g a t c a c a t t c c a c c t t t t t c c c t t t a g c t t c t t c a a t t t t a g g c a a t t t c t c a a t g a t c c c t a g t g t

5700 1170 1175 1180 1185 IMERSSFEKNPIDFLEAKGY dCas9 IMERSSFEKNPIDFLEAKGY a t t a t g g a a a g a a g t t c c t t t g a a a a a a a t c c g a t t g a c t t t t t a g a a g c t a a a g g a t a t t a a t a c c t t t c t t c a a g g a a a c t t t t t t t a g g c t a a c t g a a a a a t c t t c g a t t t c c t a t a

5760 1190 1195 1200 1205 KEVKKDLIIKLPKYSLFELE dCas9 KEVKKDLIIKLPKYSLFELE a a g g a a g t t a a a a a a g a c t t a a t c a t t a a a c t a c c t a a a t a t a g t c t t t t t g a g t t a g a a t t c c t t c a a t t t t t t c t g a a t t a g t a a t t t g a t g g a t t t a t a t c a g a a a a a c t c a a t c t t

5820 1210 1215 1220 1225 NGRKRMLASAGELQKGNELA dCas9 NGRKRMLASAGELQKGNELA a a c g g t c g t a a a c g g a t g c t g g c t a g t g c c g g a g a a t t a c a a a a a g g a a a t g a g c t g g c t t t g c c a g c a t t t g c c t a c g a c c g a t c a c g g c c t c t t a a t g t t t t t c c t t t a c t c g a c c g a

5880 1230 1235 1240 1245 LPSKYVNFLYLASHYEKLKG dCas9 LPSKYVNFLYLASHYEKLKG c t g c c a a g c a a a t a t g t g a a t t t t t t a t a t t t a g c t a g t c a t t a t g a a a a g t t g a a g g g t g a c g g t t c g t t t a t a c a c t t a a a a a a t a t a a a t c g a t c a g t a a t a c t t t t c a a c t t c c c a

5940 1250 1255 1260 1265 SPEDNEQKQLFVEQHKHYLD dCas9 SPEDNEQKQLFVEQHKHYLD a g t c c a g a a g a t a a c g a a c a a a a a c a a t t g t t t g t g g a g c a g c a t a a g c a t t a t t t a g a t t c a g g t c t t c t a t t g c t t g t t t t t g t t a a c a a a c a c c t c g t c g t a t t c g t a a t a a a t c t a

6000 1270 1275 1280 1285 EIIEQISEFSKRVILADANL dCas9 EIIEQISEFSKRVILADANL g a g a t t a t t g a g c a a a t c a g t g a a t t t t c t a a g c g t g t t a t t t t a g c a g a t g c c a a t t t a c t c t a a t a a c t c g t t t a g t c a c t t a a a a g a t t c g c a c a a t a a a a t c g t c t a c g g t t a a a t

6060 1290 1295 1300 1305 DKVLSAYNKHRDKPIREQAE dCas9 DKVLSAYNKHRDKPIREQAE g a t a a a g t t c t t a g t g c a t a t a a c a a a c a t a g a g a c a a a c c a a t a c g t g a a c a a g c a g a a c t a t t t c a a g a a t c a c g t a t a t t g t t t g t a t c t c t g t t t g g t t a t g c a c t t g t t c g t c t t

6120 1310 1315 1320 1325 NIIHLFTLTNLGAPAAFKYF dCas9 NIIHLFTLTNLGAPAAFKYF a a t a t t a t t c a t t t a t t t a c g t t g a c g a a t c t t g g a g c t c c c g c t g c t t t t a a a t a t t t t t t a t a a t a a g t a a a t a a a t g c a a c t g c t t a g a a c c t c g a g g g c g a c g a a a a t t t a t a a a a

6180 1330 1335 1340 1345 DTTIDRKRYTSTKEVLDATL dCas9 DTTIDRKRYTSTKEVLDATL g a t a c a a c a a t t g a t c g t a a a c g a t a t a c g t c t a c a a a a g a a g t t t t a g a t g c c a c t c t t c t a t g t t g t t a a c t a g c a t t t g c t a t a t g c a g a t g t t t t c t t c a a a a t c t a c g g t g a g a a

6240 1350 1355 1360 1365 IHQSITGLYETRIDLSQLGG dCas9 IHQSITGLYETRIDLSQLGG a t c c a t c a a t c c a t c a c t g g t c t t t a t g a a a c a c g c a t t g a t t t g a g t c a g c t a g g a g g t t a g g t a g t t a g g t a g t g a c c a g a a a t a c t t t g t g c g t a a c t a a a c t c a g t c g a t c c t c c a

6300 D* dCas9 D* g a c t g a a g t a t a t t t t a g a t g a a g a t t a t t t c t t a a t a a c t a a a a a t a t g g t a t a a t a c t c t g a c t t c a t a t a a a a t c t a c t t c t a a t a a a g a a t t a t t g a t t t t t a t a c c a t a t t a t g a

TspMIXmaISmaI 6360 c t t a a t a a a t g c a g t a a t a c a g g g g c t t t t c a a g a c t c c c g g g t a c c g a g c t c g a a t t c a g a a t t a t t t a c g t c a t t a t g t c c c c g a a a a g t t c t g a g g g c c c a t g g c t c g a g c t t a a g t

BssHIIAscI 6420 g g c g c g c c t a t t c t a a a t g c a t a a t a a a t a c t g a t a a c a t c t t a t a t t t t g t a t t a t a t t c c g c g c g g a t a a g a t t t a c g t a t t a t t t a t g a c t a t t g t a g a a t a t a a a a c a t a a t a t a a

6480 t t g t a t t a t c g t t g a c a t g t a t a a t t t t g a t a t c a a a a a c t g a t t t t c c c t c t a t t a t t t a a c a t a a t a g c a a c t g t a c a t a t t a a a a c t a t a g t t t t t g a c t a a a a g g g a g a t a a t a a a

6540 t c g a g a t t t a t t t t c t t a a t t c t c t t t a a c a a a c t a g a a a t a t t g t a t a t a c a a a a a a t t a g c t c t a a a t a a a a g a a t t a a g a g a a a t t g t t t g a t c t t t a t a a c a t a t a t g t t t t t t a a

6600 a t a a a t a a t a g a t g a a t a g t t t a a t t a t a g g t g t t c a t c a a t c g a a a a a g c a a c g t a t c t t a t t t a t t a t c t a c t t a t c a a a t t a a t a t c c a c a a g t a g t t a g c t t t t t c g t t g c a t a g a

6660 t a t t t a a a g t g c g t t g c t t t t t t c t c a t t t a t a a g g t t a a a t a a t t c t c a t a t a t c a a g c a t a a a t t t c a c g c a a c g a a a a a a g a g t a a a t a t t c c a a t t t a t t a a g a g t a t a t a g t t c g

6720 a a a g t g a c a g g c g c c a a a t a a t a a a a a a g c c g g a t t a a t a a t c t g g c t t t t t a t a t t c t c t t t c a c t g t c c g c g g t t t a t t a t t t t t t c g g c c t a a t t a t t a g a c c g a a a a a t a t a a g a g

6780 gRNA scaffold t g g c a g a c t a a a a a a g c a c c g a c t c g g t g c c a c t t t t t c a a g t t g a t a a c g g a c t a g c c t a c c g t c t g a t t t t t t c g t g g c t g a g c c a c g g t g a a a a a g t t c a a c t a t t g c c t g a t c g g a

6840 235 *KYLE gRNA scaffold superfolder GFP *KYLE t a t t t t a a c t t g c t a t t t c t a g c t c t a a a a c g a g a c c a t t a t c a t t a t t t g t a c a a c t c g a t a a a a t t g a a c g a t a a a g a t c g a g a t t t t g c t c t g g t a a t a g t a a t a a a c a t g t t g a g c

6900 230 225 220 215 DMGHTIGAATVFELLVMHDR superfolder GFP DMGHTIGAATVFELLVMHDR t c c a t g c c g t g c g t g a t g c c a g c t g c t g t t a c a a a c t c c a a t a a a a c c a t g t g g t c a c g t a g g t a c g g c a c g c a c t a c g g t c g a c g a c a a t g t t t g a g g t t a t t t t g g t a c a c c a g t g c a

PpuMI 6960 210 205 200 195 KENPDKSLVSQTSLYHNDPL superfolder GFP KENPDKSLVSQTSLYHNDPL t t c t c g t t c g g g t c c t t a g a t a a t a c t g a c t g t g t a g a t a a a t a g t g a t t a t c c g g c a a c a a g a g c a a g c c c a g g a a t c t a t t a t g a c t g a c a c a t c t a t t t a t c a c t a a t a g g c c g t t g

7020 190 185 180 175 LVPGDGIPTNQQYHDALQVS superfolder GFP LVPGDGIPTNQQYHDALQVS a a c a c a g g t c c g t c a c c g a t t g g c g t a t t c t g c t g g t a g t g g t c t g c t a a c t g a a c a c t t t t g t g t c c a g g c a g t g g c t a a c c g c a t a a g a c g a c c a t c a c c a g a c g a t t g a c t t g t g a a

7080 170 165 160 155 GDEVNHRIKFNAKIGNKQKD superfolder GFP GDEVNHRIKFNAKIGNKQKD c c a t c t t c t a c g t t a t g t c t t a t c t t a a a a t t t g c t t t g a t a c c a t t c t t c t g t t t g t c a g g t a g a a g a t g c a a t a c a g a a t a g a a t t t t a a a c g a a a c t a t g g t a a g a a g a c a a a c a g t

7140 150 145 140 135 ATIYVNHSNFNYELKHGLIN superfolder GFP ATIYVNHSNFNYELKHGLIN g c a g t g a t g t a c a c a t t a t g t g a g t t g a a a t t g t a t t c t a a c t t a t g t c c t a a g a t a t t g c g t c a c t a c a t g t g t a a t a c a c t c a a c t t t a a c a t a a g a t t g a a t a c a g g a t t c t a t a a c

7200 130 125 120 115 GDEKFDIGKLEIRNVLTDGE superfolder GFP GDEKFDIGKLEIRNVLTDGE c c a t c c t c t t t g a a a t c a a t a c c t t t t a a c t c a a t t c t a t t c a c c a a a g t g t c g c c c t c g g g t a g g a g a a a c t t t a g t t a t g g a a a a t t g a g t t a a g a t a a g t g g t t t c a c a g c g g g a g c

7260 110 105 100 95 FKVEARTKYTGDDKFSITRE superfolder GFP FKVEARTKYTGDDKFSITRE a a t t t c a c t t c a g c t c t t g t t t t g t a a g t a c c g t c a t c t t t g a a t g a a a t t g t a c g t t c t t t a a a g t g a a g t c g a g a a c a a a a c a t t c a t g g c a g t a g a a a c t t a c t t t a a c a t g c a a g a

7320 90 85 80 75 QVYGEPMASKFFDHRKMHDP superfolder GFP QVYGEPMASKFFDHRKMHDP t g t a c a t a a c c t t c c g g c a t a g c a c t t t t g a a a a a g t c g t g t c t c t t c a t g t g g t c t g g a a c a t g t a t t g g a a g g c c g t a t c g t g a a a a c t t t t t c a g c a c a g a g a a g t a c a c c a g a c c t

7380 70 65 60 55 YRSFCQVGYTLTTVLTPWPV superfolder GFP YRSFCQVGYTLTTVLTPWPV t a a c g a c t g a a g c a t t g g a c t c c g t a t g t t a a c g t c g t c a c t a a a g t a g g c c a t g g a a c a a t t g c t g a c t t c g t a a c c t g a g g c a t a c a a t t g c a g c a g t g a t t t c a t c c g g t a c c t t g t

7440 50 45 40 35 PLKGTTCIFKLTLKGNTADG superfolder GFP PLKGTTCIFKLTLKGNTADG g g c a a c t t a c c t g t c g t a c a a a t g a a c t t t a a t g t c a a t t t t c c g t t a g t c g c g t c g c c c c c g t t g a a t g g a c a g c a t g t t t a c t t g a a a t t a c a g t t a a a a g g c a a t c a g c g c a g c g g g

7500 30 25 20 15 EGEGRVSFKHGNVDGDLEVL superfolder GFP EGEGRVSFKHGNVDGDLEVL t c t c c t t c a c c t c g c a c a g a a a a t t t a t g g c c g t t a a c g t c a c c a t c c a a t t c t a c t a a g a g a g g a a g t g g a g c g t g t c t t t t a a a t a c c g g c a a t t g c a g t g g t a g g t t a a g a t g a t t c

7560 10 5 IPVVGTFLEEGKS 1 M superfolder GFP IPVVGTFLEEGKSM a t c g g c a c a a c a c c c g t g a a c a a c t c t t c g c c t t t t g a c a t a a a t a a t c a t c c t c c t a a t t a g c c g t g t t g t g g g c a c t t g t t g a g a a g c g g a a a a c t g t a t t t a t t a g t a g g a g g a t t a

7620 t t g a t g c a t g c a t c t t g c t c g a t a c a t t t g c c c g a t a a t a t a t t g a t a t c t a a t c t t t a t a a c t a c g t a c g t a g a a c g a g c t a t g t a a a c g g g c t a t t a t a t a a c t a t a g a t t a g a a a t a

7680 t t a t t a t a g a t a t g t t a g t c a t a a t t t t g c a t t a a a t a a g t t t t a t t a a a t a t a t t t a a t a a t a a t a t c t a t a c a a t c a g t a t t a a a a c g t a a t t t a t t c a a a a t a a t t t a t a t a a a t t a

7740 g c t c t a t t a t t t a g t t a a t t a t a a c t a a t t a a a a a t g a g a a g t a a a c a a a a a a g t g t t t c c g a g a t a a t a a a t c a a t t a a t a t t g a t t a a t t t t t a c t c t t c a t t t g t t t t t t c a c a a a g

PacI 7800 c t g c a g g g g t c t c g a g c t t a t t t t a a t t a a t c t c t a t c a a t g a t a g a g t g t t c a t a t t t t g a c g t c c c c a g a g c t c g a a t a a a a t t a a t t a g a g a t a g t t a c t a t c t c a c a a g t a t a a a a

7860 t t t t a g t t t t t c a t g a a g g c g c c t t t g c g g a a a g a g t t a a t a a g t t a a c a g a a g a t g a a c a a a a t c a a a a a g t a c t t c c g c g g a a a c g c c t t t c t c a a t t a t t c a a t t g t c t t c t a c t t g

7920 c a a a a c t a a a t g g t t t a g c a g g a a a c t t a g a t a a a a a a a t g a a t c c a g a a t t a t a t t c a g g t t t t g a t t t a c c a a a t c g t c c t t t g a a t c t a t t t t t t t a c t t a g g t c t t a a t a t a a g t c

7980 a a c a g g a a c a g c a a c a a g a a c a a c a a a a g a a t c a a a a a c g a g a t a g a g g t a t g c a c t t a t t t g t c c t t g t c g t t g t t c t t g t t g t t t t c t t a g t t t t t g c t c t a t c t c c a t a c g t g a a t a

8040 a g a a c a t g c a t t t a t g c c g a g a a a a c t t a t t g g t t g g a a t g g g c t a t g t g t t a g c t a a c t t c t t g t a c g t a a a t a c g g c t c t t t t g a a t a a c c a a c c t t a c c c g a t a c a c a a t c g a t t g a

8100 t g t t a g c g a g t t g g t t g g a c t t g a a t t g g g a t t a a t c c c a a g a a a g t a c c a a c t c a a c a a a c a a t c g c t c a a c c a a c c t g a a c t t a a c c c t a a t t a g g g t t c t t t c a t g g t t g a g t t g t t

8160 c a c a t a a a g c c c t g t a g g t t c c g a c c a a t a a g g a a a t t g g a a t a a a g c a a t a a a a g g a g t g t g t a t t t c g g g a c a t c c a a g g c t g g t t a t t c c t t t a a c c t t a t t t c g t t a t t t t c c t c a

8220 t g a a g a a a t g a a a t t c a g a g a a g c c t t t g a g a a t t t t a t a a c a a g t a a g t a t g t a c t t g g a c t t c t t t a c t t t a a g t c t c t t c g g a a a c t c t t a a a a t a t t g t t c a t t c a t a c a t g a a c c

8280 t g t t t t a g t a g t t t t a a c t g t t t a c c a g a t a a t a c a a a t g c t t a a a t a a a a a a a g a c t t g a c a a a a t c a t c a a a a t t g a c a a a t g g t c t a t t a t g t t t a c g a a t t t a t t t t t t t c t g a a c

8340 a t c t g a t t a g a c c a a a t c t t t t g a t a g t g t t a t a t t a a t a a c a a a a t a a a a a g g a g t c g c t a g a c t a a t c t g g t t t a g a a a a c t a t c a c a a t a t a a t t a t t g t t t t a t t t t t c c t c a g c g

8400 t c a c g c c c t g a c c a a a g t t t g t g a a c g a c a t c a t t c a a a g a a a a a a a c a c t g a g t t g t t t a g t g c g g g a c t g g t t t c a a a c a c t t g c t g t a g t a a g t t t c t t t t t t t g t g a c t c a a c a a a

8460 t t a t a a t c t t g t a t a t t t a g a t a t t a a a c g a t a t t t a a a t a t a c a t c a a g a t a t a t a t t t a a t a t t a g a a c a t a t a a a t c t a t a a t t t g c t a t a a a t t t a t a t g t a g t t c t a t a t a t a a a

8520 MYKN g g g t g a g c g a t t c c t t a a a c g a a a t t g a g a t t a a g g a g t c g a t t t t t t a t g t a t a a a a a c c c c a c t c g c t a a g g a a t t t g c t t t a a c t c t a a t t c c t c a g c t a a a a a a t a c a t a t t t t t g

8580 NHANHSNHLENHDLDNFSKT a a t c a t g c a a a t c a t t c a a a t c a t t t g g a a a a t c a c g a t t t a g a c a a t t t t t c t a a a a c c t t a g t a c g t t t a g t a a g t t t a g t a a a c c t t t t a g t g c t a a a t c t g t t a a a a a g a t t t t g g

8640 GYSNSRLDAHTVCISDPKLS g g c t a c t c t a a t a g c c g g t t g g a c g c a c a t a c t g t g t g c a t a t c t g a t c c a a a a t t a a g t c c g a t g a g a t t a t c g g c c a a c c t g c g t g t a t g a c a c a c g t a t a g a c t a g g t t t t a a t t c a

8700 FDAMTIVGNLNRDNAQALSK t t t g a t g c a a t g a c g a t c g t t g g a a a t c t c a a c c g a g a c a a c g c t c a a g c c c t t t c t a a a a a a c t a c g t t a c t g c t a g c a a c c t t t a g a g t t g g c t c t g t t g c g a g t t c g g g a a a g a t t t

8760 FMSVEPQIRLWDILQTKFKA t t t a t g a g t g t a g a g c c c c a a a t a a g a c t t t g g g a t a t t c t t c a a a c a a a g t t t a a a g c t a a a t a c t c a c a t c t c g g g g t t t a t t c t g a a a c c c t a t a a g a a g t t t g t t t c a a a t t t c g a

8820 KALQEKVYIEYDKVKADSWD a a a g c a c t t c a a g a a a a a g t t t a t a t t g a a t a t g a c a a a g t g a a a g c a g a t a g t t g g g a t t t t c g t g a a g t t c t t t t t c a a a t a t a a c t t a t a c t g t t t c a c t t t c g t c t a t c a a c c c t a

8880 RRNMRIEFNPNKLTRDEMIW a g a c g t a a t a t g c g t a t t g a a t t t a a t c c a a a c a a a c t t a c a c g a g a t g a a a t g a t t t g g t c t g c a t t a t a c g c a t a a c t t a a a t t a g g t t t g t t t g a a t g t g c t c t a c t t t a c t a a a c c

8940 LKQNIISYMEDDGFTRLDLA t t a a a a c a a a a t a t a a t a a g c t a c a t g g a a g a t g a c g g t t t t a c a a g a t t a g a t t t a g c c a a t t t t g t t t t a t a t t a t t c g a t g t a c c t t c t a c t g c c a a a a t g t t c t a a t c t a a a t c g g

9000 FDFEDDLSDYYAMSDKAVKK t t t g a t t t t g a a g a t g a t t t g a g t g a c t a c t a t g c a a t g t c t g a t a a a g c a g t t a a g a a a a a a c t a a a a c t t c t a c t a a a c t c a c t g a t g a t a c g t t a c a g a c t a t t t c g t c a a t t c t t t

9060 TIFYGRNGKPETKYFGVRDS a c t a t t t t t t a t g g t c g t a a t g g t a a g c c a g a a a c a a a a t a t t t t g g c g t g a g a g a t a g t t g a t a a a a a a t a c c a g c a t t a c c a t t c g g t c t t t g t t t t a t a a a a c c g c a c t c t c t a t c a

9120 NRFIRIYNKKQERKDNADAE a a t a g a t t t a t t a g a a t t t a t a a t a a a a a g c a a g a a c g t a a a g a t a a t g c a g a t g c t g a a t t a t c t a a a t a a t c t t a a a t a t t a t t t t t c g t t c t t g c a t t t c t a t t a c g t c t a c g a c t t

9180 VMSEHLWRVEIELKRDMVDY g t t a t g t c t g a a c a t t t a t g g c g t g t a g a a a t c g a a c t t a a a a g a g a t a t g g t g g a t t a c c a a t a c a g a c t t g t a a a t a c c g c a c a t c t t t a g c t t g a a t t t t c t c t a t a c c a c c t a a t g

9240 WNDCFSDLHILQPDWKTIQR t g g a a t g a t t g c t t t a g t g a t t t a c a t a t c t t g c a a c c a g a t t g g a a a a c t a t c c a a c g c a c c t t a c t a a c g a a a t c a c t a a a t g t a t a g a a c g t t g g t c t a a c c t t t t g a t a g g t t g c g

9300 TADRAIVFMLLSDEEEWGKL a c t g c g g a t a g a g c a a t a g t t t t t a t g t t a t t g a g t g a t g a a g a a g a a t g g g g a a a g c t t t g a c g c c t a t c t c g t t a t c a a a a a t a c a a t a a c t c a c t a c t t c t t c t t a c c c c t t t c g a a

XbaI 9360 HRNSRTKYKNLIKEISPVDL c a c a g a a a t t c t a g a a c a a a a t a t a a g a a t t t g a t a a a a g a a a t t t c g c c a g t c g a t t t a g t g t c t t t a a g a t c t t g t t t t a t a t t c t t a a a c t a t t t t c t t t a a a g c g g t c a g c t a a a t

BspDIClaI 9420 TDLMKSTLKANEKQLQKQID a c g g a c t t a a t g a a a t c g a c t t t a a a a g c g a a c g a a a a a c a a t t g c a a a a a c a a a t c g a t t g c c t g a a t t a c t t t a g c t g a a a t t t t c g c t t g c t t t t t g t t a a c g t t t t t g t t t a g c t a

9480 FWQHEFKFWK* t t t t g g c a a c a t g a a t t t a a a t t t t g g a a a t a g t g t a c a t a t t a a t a t t a c t g a a c a a a a a a a a c c g t t g t a c t t a a a t t t a a a a c c t t t a t c a c a t g t a t a a t t a t a a t g a c t t g t t t t

9540 a t g a t a t a t t t a a a c t a t t c t a a t t t a g g a g g a t t t t t t t a t g a a g t g t c t a t t t a a a a a t a c t a t a t a a a t t t g a t a a g a t t a a a t c c t c c t a a a a a a a t a c t t c a c a g a t a a a t t t t t

9600 t t t g g g g a a t t t a t a t g a g g t g a a a g a a t a a t t t a c c c c t a t a a a c t t t a g t c a c c t c a a a a a c c c c t t a a a t a t a c t c c a c t t t c t t a t t a a a t g g g g a t a t t t g a a a t c a g t g g a g t t

9660 g t a a a g a g g t a a a a t t g t t t a g t t t a t a t a a a a a a t t t a a a g g t t t g t t t t a t a g c g t t t c a t t t c t c c a t t t t a a c a a a t c a a a t a t a t t t t t t a a a t t t c c a a a c a a a a t a t c g c a a a

9720 t a t t t t g g c t t t g t a t t c t t t c a t t t t t t a g t g t a t t a a a t g a a a t g g t t t t a a a t g t t t a t a a a a c c g a a a c a t a a g a a a g t a a a a a a t c a c a t a a t t t a c t t t a c c a a a a t t t a c a a a

9780 c t t t a c c t g a t a t t g c a a a t c a t t t t a a t a c t a c t c c t g g a a t t a c a a a c t g g g t a a a c a g a a a t g g a c t a t a a c g t t t a g t a a a a t t a t g a t g a g g a c c t t a a t g t t t g a c c c a t t t g t

9840 c t g c a t a t a t g t t a a c t t t t t c g a t a g g a a c a g c a g t a t a t g g a a a a t t a t c t g a t t a t a g a c g t a t a t a c a a t t g a a a a a g c t a t c c t t g t c g t c a t a t a c c t t t t a a t a g a c t a a t a t

9900 t a a a t a t a a a a a a a t t g t t a a t t a t t g g t a t t a g t t t g a g c t g t c t t g g t t c a t t g a t t g a t t t a t a t t t t t t t a a c a a t t a a t a a c c a t a a t c a a a c t c g a c a g a a c c a a g t a a c t a a c

PspOMIApaIAvrII 9960 c t t t t a t t g g g c c c a c c t a g g t a t t a t c a a g a t a a g a a a g a a a a g g a t t t t t c g c t a c g c g a a a a t a a c c c g g g t g g a t c c a t a a t a g t t c t a t t c t t t c t t t t c c t a a a a a g c g a t g c g

10,020 t c a a a t c c t t t a a a a a a a c a c a a a a g a c c a c a t t t t t t a a t g t g g t c t t t t a t t c t t c a a a g t t t a g g a a a t t t t t t t g t g t t t t c t g g t g t a a a a a a t t a c a c c a g a a a a t a a g a a g t t

10,080 c t a a a g c a c c c a t t a g t t c a a c a a a c g a a a a t t g g a t a a a g t g g g a t a t t t t t a a a a t a t g a t t t c g t g g g t a a t c a a g t t g t t t g c t t t t a a c c t a t t t c a c c c t a t a a a a a t t t t a t a

10,140 a t a t t t a t g t t a c a g t a a t a t t g a c t t t t a a a a a a g g a t t g a t t c t a a t g a a g a a a g c a g t a t a a a t a c a a t g t c a t t a t a a c t g a a a a t t t t t t c c t a a c t a a g a t t a c t t c t t t c g t c

10,200 MNF a c a a g t a a g c c t c c t a a a t t c a c t t t a g a t a a a a a t t t a g g a g g c a t a t c a a a t g a a c t t t g t t c a t t c g g a g g a t t t a a g t g a a a t c t a t t t t t a a a t c c t c c g t a t a g t t t a c t t g a a

10,260 NKIDLDNWKRKEIFNHYLNQ t a a t a a a a t t g a t t t a g a c a a t t g g a a g a g a a a a g a g a t a t t t a a t c a t t a t t t g a a c c a a t t a t t t t a a c t a a a t c t g t t a a c c t t c t c t t t t c t c t a t a a a t t a g t a a t a a a c t t g g t

10,320 QTTFSITTEIDISVLYRNIK a c a a a c g a c t t t t a g t a t a a c c a c a g a a a t t g a t a t t a g t g t t t t a t a c c g a a a c a t a a a t g t t t g c t g a a a a t c a t a t t g g t g t c t t t a a c t a t a a t c a c a a a a t a t g g c t t t g t a t t t

10,380 QEGYKFYPAFIFLVTRVINS a c a a g a a g g a t a t a a a t t t t a c c c t g c a t t t a t t t t c t t a g t g a c a a g g g t g a t a a a c t c t g t t c t t c c t a t a t t t a a a a t g g g a c g t a a a t a a a a g a a t c a c t g t t c c c a c t a t t t g a g

10,440 NTAFRTGYNSDGELGYWDKL a a a t a c a g c t t t t a g a a c t g g t t a c a a t a g c g a c g g a g a g t t a g g t t a t t g g g a t a a g t t t t t a t g t c g a a a a t c t t g a c c a a t g t t a t c g c t g c c t c t c a a t c c a a t a a c c c t a t t c a a

10,500 EPLYTIFDGVSKTFSGIWTP a g a g c c a c t t t a t a c a a t t t t t g a t g g t g t a t c t a a a a c a t t c t c t g g t a t t t g g a c t c c t c t c g g t g a a a t a t g t t a a a a a c t a c c a c a t a g a t t t t g t a a g a g a c c a t a a a c c t g a g g

10,560 VKNDFKEFYDLYLSDVEKYN t g t a a a g a a t g a c t t c a a a g a g t t t t a t g a t t t a t a c c t t t c t g a t g t a g a g a a a t a t a a a c a t t t c t t a c t g a a g t t t c t c a a a a t a c t a a a t a t g g a a a g a c t a c a t c t c t t t a t a t t

10,620 GSGKLFPKTPIPENAFSLSI t g g t t c g g g g a a a t t g t t t c c c a a a a c a c c t a t a c c t g a a a a t g c t t t t t c t c t t t c t a t a c c a a g c c c c t t t a a c a a a g g g t t t t g t g g a t a t g g a c t t t t a c g a a a a a g a g a a a g a t a

10,680 IPWTSFTGFNLNINNNSNYL t a t t c c a t g g a c t t c a t t t a c t g g g t t t a a c t t a a a t a t c a a t a a t a a t a g t a a t t a c c t a t a a g g t a c c t g a a g t a a a t g a c c c a a a t t g a a t t t a t a g t t a t t a t t a t c a t t a a t g g a

10,740 LPIITAGKFINKGNSIYLPL t c t a c c c a t t a t t a c a g c a g g a a a a t t c a t t a a t a a a g g t a a t t c a a t a t a t t t a c c g c t a g a t g g g t a a t a a t g t c g t c c t t t t a a g t a a t t a t t t c c a t t a a g t t a t a t a a a t g g c g a

10,800 SLQVHHSVCDGYHAGLFMNS a t c t t t a c a g g t a c a t c a t t c t g t t t g t g a t g g t t a t c a t g c a g g a t t g t t t a t g a a c t c t a g a a a t g t c c a t g t a g t a a g a c a a a c a c t a c c a a t a g t a c g t c c t a a c a a a t a c t t g a g

StuI 10,860 IQELSDRPNDWLL* t a t t c a g g a a t t g t c a g a t a g g c c t a a t g a c t g g c t t t t a t a a t a t g a g a t a a t g c c g a c a t a a g t c c t t a a c a g t c t a t c c g g a t t a c t g a c c g a a a a t a t t a t a c t c t a t t a c g g c t g

10,920 t g t a c t t t t t a c a g t c g g t t t t c t a a t g t c a c t a a c c t g c c c c g t t a g t t g a a g a a g g c c a c a t g a a a a a t g t c a g c c a a a a g a t t a c a g t g a t t g g a c g g g g c a a t c a a c t t c t t c c g g

SacIIPspXI 10,980 g c g g c c t c g a g c g g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c c g c c g g a g c t c g c c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g

3ʹ 5ʹ 10,985 g c c c t c g g g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.