Addgene pGL0_57 [PtetL] Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pGL0_57 [PtetL] Sequencing Result


Map View

M13 Forward (2417 .. 2434) M13/pUC Forward (2403 .. 2425) BmrI (2398) BsiWI (2348) BsaAI (2306) MslI (2283) CAT-R (2128 .. 2147) PvuII (2099) BspEI (1999) ApoI (1995) BsrDI (1982) AclI (1910) BpmI (1879) PflMI * (1770) PasI (1764) MscI (1732) BtgI - NcoI (1694) SspI (1687) ScaI (1582) EcoRV (1547) SacI (1543) Eco53kI (1541) SpeI (1427) AhdI (2430) TaqII (110) AccI (375) BseRI (420) M13 Reverse (530 .. 546) BsrBI (563) M13/pUC Reverse (543 .. 565) NdeI (640) AflIII - PciI (657) DrdI (765) pBR322ori-F (798 .. 817) BssSI - BssSαI (830) BseYI (961) PspFI (965) ApaLI (971) BsiEI (997) AlwNI (1073) AcuI (1205) pGL0_57 [PtetL] 2486 bp

Sequence View

60 t a g c g t g c g a a c a g c a c g c t a t t g t t g t a t c a t t a c t c g c a t c c a t t c t c a g g c t g t c t c a t c g c a c g c t t g t c g t g c g a t a a c a a c a t a g t a a t g a g c g t a g g t a a g a g t c c g a c a g a g

TaqII 120 g t c t c g t c t c c g a g g t c t c a g g a g g g a t t t t a t g a c c g a t g a t g a a g a a a a g a a t c t g a a c a g a g c a g a g g c t c c a g a g t c c t c c c t a a a a t a c t g g c t a c t a c t t c t t t t c t t a g a c t t

180 a c t t a g t t t a t a t g t g g t a a a a t g t t t t a a t c a a g t t t a g g a g g a a t t a a t t a t g a a g t g t g a a t c a a a t a t a c a c c a t t t t a c a a a a t t a g t t c a a a t c c t c c t t a a t t a a t a c t t c a c

240 t a a t t a a t g t a a c a g g g t t c a a t t a a a a g a g g g a a g c g t a t c a t t a a c c c t a t a a a c t a c a t t a a t t a c a t t g t c c c a a g t t a a t t t t c t c c c t t c g c a t a g t a a t t g g g a t a t t t g a t g

300 g t c t g c c c t c a t t a t t g g a g g g t g a a a t a a t g t g a g a c c a c c c c a g g a t a c a t a g a t t a c c a g a c g g g a g t a a t a a c c t c c c a c t t t a t t a c a c t c t g g t g g g g t c c t a t g t a t c t a a t g

360 c a c a a c t c c g a g c c c t t c c a c c t a g c g t g c t a a c c a c g c a c g c t a t t g t t g t a t c c c a t a g t g t t g a g g c t c g g g a a g g t g g a t c g c a c g a t t g g t g c g t g c g a t a a c a a c a t a g g g t a t

BseRIAccI 420 t a a t g c a a t c g t a g t c t a c c t g a c t g t a c t t a g a a a t g t g g c t t c g c c t t t g c c c a c g c a a t t a c g t t a g c a t c a g a t g g a c t g a c a t g a a t c t t t a c a c c g a a g c g g a a a c g g g t g c g t

480 c c t g a t c g c t c c t c g t t t g c t t t t a a g g a c c g g a c g a a c c a c a g a g c a t t a g a a g g c t a g g g a c t a g c g a g g a g c a a a c g a a a a t t c c t g g c c t g c t t g g t g t c t c g t a a t c t t c c g a t c

540 M13 rev C A G T A T C G A C A M13 Reverse g t g g a g g c t c a g t g a t g a t a a g t c t g c g a t g g t g g a t g c a t g t g t c a t g g t c a t a g c t g t c a c c t c c g a g t c a c t a c t a t t c a g a c g c t a c c a c c t a c g t a c a c a g t a c c a g t a t c g a c a

BsrBI 600 M13 rev A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t t c c t g t g t g a a a t t g t t a t c c g c t c a g a g g g c a c a a t c c t a t t c c g c g c t a t c c g a c a a a a g g a c a c a c t t t a a c a a t a g g c g a g t c t c c c g t g t t a g g a t a a g g c g c g a t a g g c t g t t

AflIIIPciINdeI 660 t c t c c a a g a c a t t a g g t g g a g t t c a g t t c g g c g t a t g g c a t a t g t c g c t g g a a a g a a c a t a g a g g t t c t g t a a t c c a c c t c a a g t c a a g c c g c a t a c c g t a t a c a g c g a c c t t t c t t g t a

720 ori g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a

DrdI 780 ori c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c

BssSαIBssSI 840 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g

900 ori t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a

960 ori g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t

BsiEIApaLIBseYIPspFI 1020 ori g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t

AlwNI 1080 ori t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t

1140 ori c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t

1200 ori c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a

AcuI 1260 ori c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a

1320 ori t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a

1380 c t t t t c t a c g g g g t c t g a c g c t c t a t t c a a c a a a g c c g c c g t c c c g t c a a g t c a g c g t a a g a a a a g a t g c c c c a g a c t g c g a g a t a a g t t g t t t c g g c g g c a g g g c a g t t c a g t c g c a t t

SpeI 1440 lambda t0 terminator a t g g g t a g g g g g c t t c a a a t c g t c c c c c c a t a c g a t a t a a g t t g t t a c t a g t g c t t g g a t t a c c c a t c c c c c g a a g t t t a g c a g g g g g g t a t g c t a t a t t c a a c a a t g a t c a c g a a c c t a

1500 lambda t0 terminator t c t c a c c a a t a a a a a a c g c c c g g c g g c a a c c g a g c g t t c t g a a c a a a t c c a g a t g g a g t t a g a g t g g t t a t t t t t t g c g g g c c g c c g t t g g c t c g c a a g a c t t g t t t a g g t c t a c c t c a a

EcoRVEco53kISacI 1560 220 *A lambda t0 terminator CmR *A c t g a g g t c a t t a c t g g a t c t a t c a a c a g g a g t c c a a g c g a g c t c g a t a t c a a a t t a c g c c g a c t c c a g t a a t g a c c t a g a t a g t t g t c c t c a g g t t c g c t c g a g c t a t a g t t t a a t g c g g

ScaI 1620 215 210 205 200 GGQWEDCYQQLENLMRGVHF CmR GGQWEDCYQQLENLMRGVHF c c g c c c t g c c a c t c a t c g c a g t a c t g t t g t a a t t c a t t a a g c a t t c t g c c g a c a t g g a a g g g c g g g a c g g t g a g t a g c g t c a t g a c a a c a t t a a g t a a t t c g t a a g a c g g c t g t a c c t t c

1680 195 190 185 180 GDCVAHHVQIALPMLVKDGQ CmR GDCVAHHVQIALPMLVKDGQ c c a t c a c a a a c g g c a t g a t g a a c c t g a a t c g c c a g c g g c a t c a g c a c c t t g t c g c c t t g c g g t a g t g t t t g c c g t a c t a c t t g g a c t t a g c g g t c g c c g t a g t c g t g g a a c a g c g g a a c g

MscINcoIBtgISspI 1740 175 170 165 160 TYYKGMTFVPAFFNDMNAVN CmR TYYKGMTFVPAFFNDMNAVN g t a t a a t a t t t g c c c a t g g t g a a a a c g g g g g c g a a g a a g t t g t c c a t a t t g g c c a c g t t t c a t a t t a t a a a c g g g t a c c a c t t t t g c c c c c g c t t c t t c a a c a g g t a t a a c c g g t g c a a a

PflMI*PasI 1800 155 150 145 140 LDFSTFSVWPNASVFFMNEI CmR LDFSTFSVWPNASVFFMNEI a a a t c a a a a c t g g t g a a a c t c a c c c a g g g a t t g g c t g a g a c g a a a a a c a t a t t c t c a a t a t t t a g t t t t g a c c a c t t t g a g t g g g t c c c t a a c c g a c t c t g c t t t t t g t a t a a g a g t t a t

1860 135 130 125 120 FGKPFYALNEGYCAVDQSYI CmR FGKPFYALNEGYCAVDQSYI a a c c c t t t a g g g a a a t a g g c c a g g t t t t c a c c g t a a c a c g c c a c a t c t t g c g a a t a t a t g t t g g g a a a t c c c t t t a t c c g g t c c a a a a g t g g c a t t g t g c g g t g t a g a a c g c t t a t a t a c

AclIBpmI 1920 115 110 105 100 HLFQRFDDHYESWLSSFTET CmR HLFQRFDDHYESWLSSFTET t g t a g a a a c t g c c g g a a a t c g t c g t g g t a t t c a c t c c a g a g c g a t g a a a a c g t t t c a g t t a c a t c t t t g a c g g c c t t t a g c a g c a c c a t a a g t g a g g t c t c g c t a c t t t t g c a a a g t c a a

1980 95 90 85 80 QEHFVTYCPHVSDWIVLEGD CmR QEHFVTYCPHVSDWIVLEGD t g c t c a t g g a a a a c g g t g t a a c a a g g g t g a a c a c t a t c c c a t a t c a c c a g c t c a c c g t c t a c g a g t a c c t t t t g c c a c a t t g t t c c c a c t t g t g a t a g g g t a t a g t g g t c g a g t g g c a g a

BspEIApoIBsrDI 2040 75 70 65 60 KMAMRFEPHANMLRALIHIF CmR KMAMRFEPHANMLRALIHIF t t c a t t g c c a t a c g a a a t t c c g g a t g a g c a t t c a t c a g g c g g g c a a g a a t g t g a a t a a a g a a g t a a c g g t a t g c t t t a a g g c c t a c t c g t a a g t a g t c c g c c c g t t c t t a c a c t t a t t t c

PvuII 2100 55 50 45 40 APYFKHKNKKVTKLFATIDL CmR APYFKHKNKKVTKLFATIDL g c c g g a t a a a a c t t g t g c t t a t t t t t c t t t a c g g t c t t t a a a a a g g c c g t a a t a t c c a g c c g g c c t a t t t t g a a c a c g a a t a a a a a g a a a t g c c a g a a a t t t t t c c g g c a t t a t a g g t c g

2160 35 30 25 20 QVTQNYTCQAVSQFAEFHEK CmR G C A A C T G A C T G A A A T G C C T C CAT-R QVTQNYTCQAVSQFAEFHEK t g a a c g g t c t g g t t a t a g g t a c a t t g a g c a a c t g a c t g a a a t g c c t c a a a a t g t t c t t t a a c t t g c c a g a c c a a t a t c c a t g t a a c t c g t t g a c t g a c t t t a c g g a g t t t t a c a a g a a a t

2220 15 10 5 1 RHWQSIDVTTYGTIKKEM CmR cat promoter RHWQSIDVTTYGTIKKEM c g a t g c c a t t g g g a t a t a t c a a c g g t g g t a t a t c c a g t g a t t t t t t t c t c c a t t t t a g c t g c t a c g g t a a c c c t a t a t a g t t g c c a c c a t a t a g g t c a c t a a a a a a a g a g g t a a a a t c g a

2280 cat promoter t c c t t a g c t c c t g a a a a t c t c g a t a a c t c a a a a a a t a c g c c c g g t a g t g a t c t t a t t t c a a g g a a t c g a g g a c t t t t a g a g c t a t t g a g t t t t t t a t g c g g g c c a t c a c t a g a a t a a a g t

BsaAIMslI 2340 cat promoter t t a t g g t g a a a g t t g g a a c c t c t t a c g t g c c g a t c a a c g t c t c a t t g a t a c c t g a a a c a a a a t a c c a c t t t c a a c c t t g g a g a a t g c a c g g c t a g t t g c a g a g t a a c t a t g g a c t t t g t t

BmrIBsiWI 2400 a a c c c a t c g t a c g g c c a a g g a a g t c t c c a a t a a c t g t g a t c c a c c a c a a g c g c c a g g g t t t t g g g t a g c a t g c c g g t t c c t t c a g a g g t t a t t g a c a c t a g g t g g t g t t c g c g g t c c c a a

AhdI 2460 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t c a t g c a t a a t c c g c a c g c a t c t g g a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a g t a c g t a t t a g g c g t g c g t a g a c c t t

2486 t a a g g a a g t g c c a t t c c g c c t g a c c t a t t c c t t c a c g g t a a g g c g g a c t g g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.