Addgene pEG302-HD2A-ZF Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pEG302-HD2A-ZF Sequencing Result


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pBR322ori-F (490 .. 509) AvrII (14,574) StuI (14,234) NsiI (13,910) LB T-DNA repeat SacI (12,579) Eco53kI (12,577) FspAI (12,157) PaqCI (12,089) EcoRI (11,352) attB1 AatII (11,108) ZraI (11,106) BamHI (9201) HRV 3C site Bsu36I (8782) PspXI (8667) MfeI (8234) OCS terminator L4440 (743 .. 760) pRS-marker (841 .. 860) pGEX 3' (1001 .. 1023) KasI (1071) NarI (1072) SfoI (1073) PluTI (1075) BsaI (1453) PmeI (4989) M13/pUC Forward (5163 .. 5185) M13 Forward (5177 .. 5194) M13 fwd BstXI (6023) DraIII (6232) BstEII (7961) pEG302-HD2A-ZF 15,321 bp

Sequence View

5ʹ 3ʹ 60 ori t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g

120 ori a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a

180 ori c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a

240 ori c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g

300 ori t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t

360 ori g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g

420 ori c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c

480 ori g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t

540 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a

600 ori t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t

660 c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g

720 g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c

780 bom A G C G A G T C A G T G A G C G A G L4440 c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a

840 bom g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c t c t c a g c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t g a g a g t c

900 bom A T G T T A G A C G A G A C T A C G G C pRS-marker t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t a c a c t c c g c t a t c g c t a c g t g a c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a t g t g a g g c g a t a g c g a t g c a c t g

960 bom t g g g t c a t g g c t g c g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t a c c c a g t a c c g a c g c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a

1020 C C G G G A G C T G C A T G T G T C A G pGEX 3' c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c

KasINarISfoIPluTI 1080 A G G pGEX 3' a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g g c a g g g t g c c t t g a t g t g g g c g c c g g c g t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c c g t c c c a c g g a a c t a c a c c c g c g g c c g c

1140 g t c g a g t g g c g a c g g c g c g g c t t g t c c g c g c c c t g g t a g a t t g c c t g g c c g t a g g c c a g c c a g c t c a c c g c t g c c g c g c c g a a c a g g c g c g g g a c c a t c t a a c g g a c c g g c a t c c g g t c g

1200 c a t t t t t g a g c g g c c a g c g g c c g c g a t a g g c c g a c g c g a a g c g g c g g g g c g t a g g g a g c g g t a a a a a c t c g c c g g t c g c c g g c g c t a t c c g g c t g c g c t t c g c c g c c c c g c a t c c c t c g c

1260 pVS1 oriV c a g c g a c c g a a g g g t a g g c g c t t t t t g c a g c t c t t c g g c t g t g c g c t g g c c a g a c a g t t a g t c g c t g g c t t c c c a t c c g c g a a a a a c g t c g a g a a g c c g a c a c g c g a c c g g t c t g t c a a t

1320 pVS1 oriV t g c a c a g g c c a g g c g g g t t t t a a g a g t t t t a a t a a g t t t t a a a g a g t t t t a g g c g g a a a a a c g t g t c c g g t c c g c c c a a a a t t c t c a a a a t t a t t c a a a a t t t c t c a a a a t c c g c c t t t t

1380 pVS1 oriV a t c g c c t t t t t t c t c t t t t a t a t c a g t c a c t t a c a t g t g t g a c c g g t t c c c a a t g t a c g g t a g c g g a a a a a a g a g a a a a t a t a g t c a g t g a a t g t a c a c a c t g g c c a a g g g t t a c a t g c c

1440 pVS1 oriV c t t t g g g t t c c c a a t g t a c g g g t t c c g g t t c c c a a t g t a c g g c t t t g g g t t c c c a a t g t a g a a a c c c a a g g g t t a c a t g c c c a a g g c c a a g g g t t a c a t g c c g a a a c c c a a g g g t t a c a t

BsaI 1500 pVS1 oriV c g t g c t a t c c a c a g g a a a g a g a c c t t t t c g a c c t t t t t c c c c t g c t a g g g c a a t t t g c c c g c a c g a t a g g t g t c c t t t c t c t g g a a a a g c t g g a a a a a g g g g a c g a t c c c g t t a a a c g g g

1560 355 350 345 *SGASAEGEILNRY pVS1 RepA *SGASAEGEILNRY t a g c a t c t g c t c c g t a c a t t a g g a a c c g g c g g a t g c t t c g c c c t c g a t c a g g t t g c g g t a a t c g t a g a c g a g g c a t g t a a t c c t t g g c c g c c t a c g a a g c g g g a g c t a g t c c a a c g c c a t

1620 340 335 330 325 RMVLIPGAQGAEEKLDYEPL pVS1 RepA RMVLIPGAQGAEEKLDYEPL g c g c a t g a c t a g g a t c g g g c c a g c c t g c c c c g c c t c c t c c t t c a a a t c g t a c t c c g g c a g c g c g t a c t g a t c c t a g c c c g g t c g g a c g g g g c g g a g g a g g a a g t t t a g c a t g a g g c c g t c

1680 320 315 310 305 DNSGILKRVTFCFKKFEGAS pVS1 RepA DNSGILKRVTFCFKKFEGAS g t c a t t t g a c c c g a t c a g c t t g c g c a c g g t g a a a c a g a a c t t c t t g a a c t c t c c g g c g c t c a g t a a a c t g g g c t a g t c g a a c g c g t g c c a c t t t g t c t t g a a g a a c t t g a g a g g c c g c g a

1740 300 295 290 285 GSREYITKFLWRAEAKGAAR pVS1 RepA GSREYITKFLWRAEAKGAAR g c c a c t g c g t t c g t a g a t c g t c t t g a a c a a c c a t c t g g c t t c t g c c t t g c c t g c g g c g c g c g g t g a c g c a a g c a t c t a g c a g a a c t t g t t g g t a g a c c g a a g a c g g a a c g g a c g c c g c g c

1800 280 275 270 265 RALRYLFRGIGPDILFYDPH pVS1 RepA RALRYLFRGIGPDILFYDPH g c g t g c c a g g c g g t a g a g a a a a c g g c c g a t g c c g g g a t c g a t c a a a a a g t a a t c g g g g t g c g c a c g g t c c g c c a t c t c t t t t g c c g g c t a c g g c c c t a g c t a g t t t t t c a t t a g c c c c a c

1860 260 255 250 245 VTLVDPNKGETIERYMWDAL pVS1 RepA VTLVDPNKGETIERYMWDAL a a c c g t c a g c a c g t c c g g g t t c t t g c c t t c t g t g a t c t c g c g g t a c a t c c a a t c a g c t a g t t g g c a g t c g t g c a g g c c c a a g a a c g g a a g a c a c t a g a g c g c c a t g t a g g t t a g t c g a t c

1920 240 235 230 225 EIEIYEPRGTESKVIKYRSI pVS1 RepA EIEIYEPRGTESKVIKYRSI c t c g a t c t c g a t g t a c t c c g g c c g c c c g g t t t c g c t c t t t a c g a t c t t g t a g c g g c t a a t g a g c t a g a g c t a c a t g a g g c c g g c g g g c c a a a g c g a g a a a t g c t a g a a c a t c g c c g a t t a

1980 220 215 210 205 LAEGESVTVLRGNKAKKTRQ pVS1 RepA LAEGESVTVLRGNKAKKTRQ c a a g g c t t c a c c c t c g g a t a c c g t c a c c a g g c g g c c g t t c t t g g c c t t c t t c g t a c g c t g g t t c c g a a g t g g g a g c c t a t g g c a g t g g t c c g c c g g c a a g a a c c g g a a g a a g c a t g c g a c

2040 200 195 190 185 MAVHTTNLRICTEVLDDKQK pVS1 RepA MAVHTTNLRICTEVLDDKQK c a t g g c a a c g t g c g t g g t g t t t a a c c g a a t g c a g g t t t c t a c c a g g t c g t c t t t c t g c t t g t a c c g t t g c a c g c a c c a c a a a t t g g c t t a c g t c c a a a g a t g g t c c a g c a g a a a g a c g a a

2100 180 175 170 165 GGDARRCFKLVDAVHPRFVR pVS1 RepA GGDARRCFKLVDAVHPRFVR t c c g c c a t c g g c t c g c c g g c a g a a c t t g a g t a c g t c c g c a a c g t g t g g a c g g a a c a c g c g a g g c g g t a g c c g a g c g g c c g t c t t g a a c t c a t g c a g g c g t t g c a c a c c t g c c t t g t g c g c

2160 160 155 150 145 GPKDGKGERYRNMSETLHSV pVS1 RepA MGN GPKDGKGERYRNMSETLHSV g c c g g g c t t g t c t c c c t t c c c t t c c c g g t a t c g g t t c a t g g a t t c g g t t a g a t g g g a a a c c g g c c c g a a c a g a g g g a a g g g a a g g g c c a t a g c c a a g t a c c t a a g c c a a t c t a c c c t t t g

2220 140 135 130 125 AMLVLDYDWVSAMGAPGASV pVS1 RepA RHQYQVVIPHTGHAGRPCGN AMLVLDYDWVSAMGAPGASV c g c c a t c a g t a c c a g g t c g t a a t c c c a c a c a c t g g c c a t g c c g g c c g g c c c t g c g g a a a c g c g g t a g t c a t g g t c c a g c a t t a g g g t g t g t g a c c g g t a c g g c c g g c c g g g a c g c c t t t g

2280 120 115 110 105 EVHGDPLEYRIVEGARRDRK pVS1 RepA LYVPVWKLVADHLASSSVTL EVHGDPLEYRIVEGARRDRK c t c t a c g t g c c c g t c t g g a a g c t c g t a g c g g a t c a c c t c g c c a g c t c g t c g g t c a c g c t t g a g a t g c a c g g g c a g a c c t t c g a g c a t c g c c t a g t g g a g c g g t c g a g c a g c c a g t g c g a a

2340 100 95 90 85 SLRFVAVDMISRSDRTGVDY pVS1 RepA RQTENGHVHDAATIAGAHVI SLRFVAVDMISRSDRTGVDY c g a c a g a c g g a a a a c g g c c a c g t c c a t g a t g c t g c g a c t a t c g c g g g t g c c c a c g t c a t a g c t g t c t g c c t t t t g c c g g t g c a g g t a c t a c g a c g c t g a t a g c g c c c a c g g g t g c a g t a t

2400 80 75 70 65 LMPVFFDPQEDGKPPKRISP pVS1 RepA EHRNEKIWLLVALGRLPNRR LMPVFFDPQEDGKPPKRISP g a g c a t c g g a a c g a a a a a a t c t g g t t g c t c g t c g c c c t t g g g c g g c t t c c t a a t c g a c g g c t c g t a g c c t t g c t t t t t t a g a c c a a c g a g c a g c g g g a a c c c g c c g a a g g a t t a g c t g c c

2460 60 55 50 45 AGAAPPQRSEKRIRDAAAQW pVS1 RepA RTGCRRLPGFFADSISGRLP AGAAPPQRSEKRIRDAAAQW c g c a c c g g c t g c c g g c g g t t g c c g g g a t t c t t t g c g g a t t c g a t c a g c g g c c g c t t g c c a g c g t g g c c g a c g g c c g c c a a c g g c c c t a a g a a a c g c c t a a g c t a g t c g c c g g c g a a c g g t

2520 40 35 30 25 SEGPRAEAEIRQRQAAQAAK pVS1 RepA RFTGACFCLDALPLGGLRGL SEGPRAEAEIRQRQAAQAAK c g a t t c a c c g g g g c g t g c t t c t g c c t c g a t g c g t t g c c g c t g g g c g g c c t g c g c g g c c t t g c t a a g t g g c c c c g c a c g a a g a c g g a g c t a c g c a a c g g c g a c c c g c c g g a c g c g c c g g a a

2580 20 15 10 5 LKEVLDDGLAAGIQVPGSPK pVS1 RepA QLLHQVITQRRADLYRAGWF LKEVLDDGLAAGIQVPGSPK c a a c t t c t c c a c c a g g t c a t c a c c c a g c g c c g c g c c g a t t t g t a c c g g g c c g g a t g g t t t g t t g a a g a g g t g g t c c a g t a g t g g g t c g c g g c g c g g c t a a a c a t g g c c c g g c c t a c c a a a

2640 1 RGSM pVS1 RepA ATAHADSSGLGVPVPLQGRQ RGSVGIGRAQPNWHWQLAPL g c g a c c g c t c a c g c c g a t t c c t c g g g c t t g g g g g t t c c a g t g c c a t t g c a g g g c c g g c a g c g c t g g c g a g t g c g g c t a a g g a g c c c g a a c c c c c a a g g t c a c g g t a a c g t c c c g g c c g t c

2700 ATQPLTPGQPPVPPHMGHST CGLRKRRALRGNRWVHPM g c a a c c c a g c c g c t t a c g c c t g g c c a a c c g c c c g t t c c t c c a c a c a t g g g g c a t t c c a c g c g t t g g g t c g g c g a a t g c g g a c c g g t t g g c g g g c a a g g a g g t g t g t a c c c c g t a a g g t g c

2760 ASVPGCS* g c g t c g g t g c c t g g t t g t t c t t g a t t t t c c a t g c c g c c t c c t t t a g c c g c t a a a a t t c a t c g c a g c c a c g g a c c a a c a a g a a c t a a a a g g t a c g g c g g a g g a a a t c g g c g a t t t t a a g t a

2820 c t a c t c a t t t a t t c a t t t g c t c a t t t a c t c t g g t a g c t g c g c g a t g t a t t c a g a t a g c a g g a t g a g t a a a t a a g t a a a c g a g t a a a t g a g a c c a t c g a c g c g c t a c a t a a g t c t a t c g t c

2880 c t c g g t a a t g g t c t t g c c t t g g c g t a c c g c g t a c a t c t t c a g c t t g g t g t g a t c c t c c g c g a g c c a t t a c c a g a a c g g a a c c g c a t g g c g c a t g t a g a a g t c g a a c c a c a c t a g g a g g c g

2940 c g g c a a c t g a a a g t t g a c c c g c t t c a t g g c t g g c g t g t c t g c c a g g c t g g c c a a c g t t g c g c c g t t g a c t t t c a a c t g g g c g a a g t a c c g a c c g c a c a g a c g g t c c g a c c g g t t g c a a c g

3000 a g c c t t g c t g c t g c g t g c g c t c g g a c g g c c g g c a c t t a g c g t g t t t g t g c t t t t g c t c a t t c g g a a c g a c g a c g c a c g c g a g c c t g c c g g c c g t g a a t c g c a c a a a c a c g a a a a c g a g t a

3060 210 205 200 *ILKSKIEAALAQ pVS1 StaA *ILKSKIEAALAQ t t t c t c t t t a c c t c a t t a a c t c a a a t g a g t t t t g a t t t a a t t t c a g c g g c c a g c g c c t g g a a a g a g a a a t g g a g t a a t t g a g t t t a c t c a a a a c t a a a t t a a a g t c g c c g g t c g c g g a c c

3120 195 190 185 180 VERAADGEPESELVTTGAAA pVS1 StaA VERAADGEPESELVTTGAAA a c c t c g c g g g c a g c g t c g c c c t c g g g t t c t g a t t c a a g a a c g g t t g t g c c g g c g g c g g c a t g g a g c g c c c g t c g c a g c g g g a g c c c a a g a c t a a g t t c t t g c c a a c a c g g c c g c c g c c g t

3180 175 170 165 160 TGPYSVRQTIRSELIPLEYG pVS1 StaA TGPYSVRQTIRSELIPLEYG g t g c c t g g g t a g c t c a c g c g c t g c g t g a t a c g g g a c t c a a g a a t g g g c a g c t c g t a c c c g c a c g g a c c c a t c g a g t g c g c g a c g c a c t a t g c c c t g a g t t c t t a c c c g t c g a g c a t g g g c

3240 155 150 145 140 ALAEAVEGGIRTGKIARSVV pVS1 StaA ALAEAVEGGIRTGKIARSVV g c c a g c g c c t c g g c a a c c t c a c c g c c g a t g c g c g t g c c t t t g a t c g c c c g c g a c a c g a c a c g g t c g c g g a g c c g t t g g a g t g g c g g c t a c g c g c a c g g a a a c t a g c g g g c g c t g t g c t g t

3300 135 130 125 120 FAAQLRGDTVEIRQKVLEVL pVS1 StaA FAAQLRGDTVEIRQKVLEVL a a g g c c g c t t g t a g c c t t c c a t c c g t g a c c t c a a t g c g c t g c t t a a c c a g c t c c a c c a g g t t c c g g c g a a c a t c g g a a g g t a g g c a c t g g a g t t a c g c g a c g a a t t g g t c g a g g t g g t c c

3360 115 110 105 100 DATAWIDYPSPQVPILVFDA pVS1 StaA DATAWIDYPSPQVPILVFDA t c g g c g g t g g c c c a t a t g t c g t a a g g g c t t g g c t g c a c c g g a a t c a g c a c g a a g t c g g c t a g c c g c c a c c g g g t a t a c a g c a t t c c c g a a c c g a c g t g g c c t t a g t c g t g c t t c a g c c g a

3420 95 90 85 80 AKIASVALDAAQPAGDIVVF pVS1 StaA AKIASVALDAAQPAGDIVVF g c c t t g a t c g c g g a c a c a g c c a a g t c c g c c g c c t g g g g c g c t c c g t c g a t c a c t a c g a a g c g g a a c t a g c g c c t g t g t c g g t t c a g g c g g c g g a c c c c g c g a g g c a g c t a g t g a t g c t t c

3480 75 70 65 60 DRRGIAKVDRDITPRDIGVV pVS1 StaA DRRGIAKVDRDITPRDIGVV t c g c g c c g g c c g a t g g c c t t c a c g t c g c g g t c a a t c g t c g g g c g g t c g a t g c c g a c a a c g a g c g c g g c c g g c t a c c g g a a g t g c a g c g c c a g t t a g c a g c c c g c c a g c t a c g g c t g t t g c

3540 55 50 45 40 TLPQDERVAAWDRASGQPDS pVS1 StaA TLPQDERVAAWDRASGQPDS g t t a g c g g t t g a t c t t c c c g c a c g g c c g c c c a a t c g c g g g c a c t g c c c t g g g g a t c g g a a c a a t c g c c a a c t a g a a g g g c g t g c c g g c g g g t t a g c g c c c g t g a c g g g a c c c c t a g c c t t

3600 35 30 25 20 DVLLVDAGALQLARALHTAI pVS1 StaA DVLLVDAGALQLARALHTAI t c g a c t a a c a g a a c a t c g g c c c c g g c g a g t t g c a g g g c g c g g g c t a g a t g g g t t g c g a t g a g c t g a t t g t c t t g t a g c c g g g g c c g c t c a a c g t c c c g c g c c c g a t c t a c c c a a c g c t a c

3660 15 10 5 1 TTKGSGGKQNLVAIVKM pVS1 StaA TTKGSGGKQNLVAIVKM g t c g t c t t g c c t g a c c c g c c t t t c t g g t t a a g t a c a g c g a t a a c c t t c a t g c g t t c c c c t c a g c a g a a c g g a c t g g g c g g a a a g a c c a a t t c a t g t c g c t a t t g g a a g t a c g c a a g g g g a

3720 t g c g t a t t t g t t t a t t t a c t c a t c g c a t c a t a t a c g c a g c g a c c g c a t g a c g c a a g c t g t a c g c a t a a a c a a a t a a a t g a g t a g c g t a g t a t a t g c g t c g c t g g c g t a c t g c g t t c g a c a

3780 t t t a c t c a a a t a c a c a t c a c c t t t t t a g a c g g c g g c g c t c g g t t t c t t c a g c g g c c a a g c a a a t g a g t t t a t g t g t a g t g g a a a a a t c t g c c g c c g c g a g c c a a a g a a g t c g c c g g t t c g

3840 t g g c c g g c c a g g c c g c c a g c t t g g c a t c a g a c a a a c c g g c c a g g a t t t c a t g c a g c c g c a a c c g g c c g g t c c g g c g g t c g a a c c g t a g t c t g t t t g g c c g g t c c t a a a g t a c g t c g g c g t

3900 c g g t t g a g a c g t g c g c g g g c g g c t c g a a c a c g t a c c c g g c c g c g a t c a t c t c c g c c t c g a g c c a a c t c t g c a c g c g c c c g c c g a g c t t g t g c a t g g g c c g g c g c t a g t a g a g g c g g a g c t

3960 *AQE t c t c t t c g g t a a t g a a a a a c g g t t c g t c c t g g c c g t c c t g g t g c g g t t t c a t g c t t g t t c a g a g a a g c c a t t a c t t t t t g c c a a g c a g g a c c g g c a g g a c c a c g c c a a a g t a c g a a c a a g

4020 EQRENEAAALADAETLADER c t c t t g g c g t t c a t t c t c g g c g g c c g c c a g g g c g t c g g c c t c g g t c a a t g c g t c c t c a c g g a g a a c c g c a a g t a a g a g c c g c c g g c g g t c c c g c a g c c g g a g c c a g t t a c g c a g g a g t g c

4080 FAGRRRAETPTVEESRELAR g a a g g c a c c g c g c c g c c t g g c c t c g g t g g g c g t c a c t t c c t c g c t g c g c t c a a g t g c g c g c t t c c g t g g c g c g g c g g a c c g g a g c c a c c c g c a g t g a a g g a g c g a c g c g a g t t c a c g c g c

4140 YLTSRHVGLLAAAEKVTRGE g t a c a g g g t c g a g c g a t g c a c g c c a a g c a g t g c a g c c g c c t c t t t c a c g g t g c g g c c t t c c a t g t c c c a g c t c g c t a c g t g c g g t t c g t c a c g t c g g c g g a g a a a g t g c c a c g c c g g a a g

4200 QDILERAHAIQAPTLTPRPG c t g g t c g a t c a g c t c g c g g g c g t g c g c g a t c t g t g c c g g g g t g a g g g t a g g g c g g g g g c c g a c c a g c t a g t c g a g c g c c c g c a c g c g c t a g a c a c g g c c c c a c t c c c a t c c c g c c c c c g g

4260 FKVGRAKAAERGSRTRDIIL a a a c t t c a c g c c t c g g g c c t t g g c g g c c t c g c g c c c g c t c c g g g t g c g g t c g a t g a t t a g t t t g a a g t g c g g a g c c c g g a a c c g c c g g a g c g c g g g c g a g g c c c a c g c c a g c t a c t a a t c

4320 SREFEAIGAFVTLVMRGAPT g g a a c g c t c g a a c t c g g c a a t g c c g g c g a a c a c g g t c a a c a c c a t g c g g c c g g c c g g c g t c c t t g c g a g c t t g a g c c g t t a c g g c c g c t t g t g c c a g t t g t g g t a c g c c g g c c g g c c g c a

4380 TTDAWPEALSRLGAGAEQIR g g t g g t g t c g g c c c a c g g c t c t g c c a g g c t a c g c a g g c c c g c g c c g g c c t c c t g g a t g c g c c a c c a c a g c c g g g t g c c g a g a c g g t c c g a t g c g t c c g g g c g c g g c c g g a g g a c c t a c g c

4440 EAIDLLDRTSRALRDLRTVT c t c g g c a a t g t c c a g t a g g t c g c g g g t g c t g c g g g c c a g g c g g t c t a g c c t g g t c a c t g t g a g c c g t t a c a g g t c a t c c a g c g c c c a c g a c g c c c g g t c c g c c a g a t c g g a c c a g t g a c a

4500 VVDGPRLHDLMRALEPRDRR c a c a a c g t c g c c a g g g c g t a g g t g g t c a a g c a t c c t g g c c a g c t c c g g g c g g t c g c g c c t g t g t t g c a g c g g t c c c g c a t c c a c c a g t t c g t a g g a c c g g t c g a g g c c c g c c a g c g c g g a

4560 TGTIKESFLKTCGAAHLEAR g g t g c c g g t g a t c t t c t c g g a a a a c a g c t t g g t g c a g c c g g c c g c g t g c a g t t c g g c c c g c c a c g g c c a c t a g a a g a g c c t t t t g t c g a a c c a c g t c g g c c g g c g c a c g t c a a g c c g g g c

4620 QNTLDQDDTSVRAYGLLGAA t t g g t t g g t c a a g t c c t g g t c g t c g g t g c t g a c g c g g g c a t a g c c c a g c a g g c c a g c g g c a a c c a a c c a g t t c a g g a c c a g c a g c c a c g a c t g c g c c c g t a t c g g g t c g t c c g g t c g c c g

4680 ASKNM g g c g c t c t t g t t c a t g g c g t a a t g t c t c c g g t t c t a g t c g c a a g t a t t c t a c t t t a t g c g c c g c g a g a a c a a g t a c c g c a t t a c a g a g g c c a a g a t c a g c g t t c a t a a g a t g a a a t a c g c

4740 a c t a a a a c a c g c g a c a a g a a a a c g c c a g g a a a a g g g c a g g g c g g c a g c c t g t c g c g t a a c t g a t t t t g t g c g c t g t t c t t t t g c g g t c c t t t t c c c g t c c c g c c g t c g g a c a g c g c a t t g

4800 t t a g g a c t t g t g c g a c a t g t c g t t t t c a g a a g a c g g c t g c a c t g a a c g t c a g a a g c c g a c a a t c c t g a a c a c g c t g t a c a g c a a a a g t c t t c t g c c g a c g t g a c t t g c a g t c t t c g g c t g

4860 t g c a c t a t a g c a g c g g a g g g g t t g g a t c a a a g t a c t t t g a t c c c g a g g g g a a c c c t g t g g a c g t g a t a t c g t c g c c t c c c c a a c c t a g t t t c a t g a a a c t a g g g c t c c c c t t g g g a c a c c

4920 t t g g c a t g c a c a t a c a a a t g g a c g a a c g g a t a a a c c t t t t c a c g c c c t t t t a a a t a t c c g a a c c g t a c g t g t a t g t t t a c c t g c t t g c c t a t t t g g a a a a g t g c g g g a a a a t t t a t a g g c

4980 RB T-DNA repeat a t t a t t c t a a t a a a c g c t c t t t t c t c t t a g g t t t a c c c g c c a a t a t a t c c t g t c a a a c a c t a a t a a g a t t a t t t g c g a g a a a a g a g a a t c c a a a t g g g c g g t t a t a t a g g a c a g t t t g t g

PmeI 5040 t g a t a g t t t a a a c t g a a g g c g g g a a a c g a c a a t c t g a t c c a a g c t c a a g c t g c t c t a g c a a c t a t c a a a t t t g a c t t c c g c c c t t t g c t g t t a g a c t a g g t t c g a g t t c g a c g a g a t c g t

5100 t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a

5160 a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t t t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a a

5220 M13 fwd CaMV poly(A) signal C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g c c a a g c t t g c a t g c c t g c a g g g a t c a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c g g t t c g a a c g t a c g g a c g t c c c t a g

5280 CaMV poly(A) signal t g g a t t t t a g t a c t g g a t t t t g g t t t t a g g a a t t a g a a a t t t t a t t g a t a g a a g t a t t t t a c c t a a a a t c a t g a c c t a a a a c c a a a a t c c t t a a t c t t t a a a a t a a c t a t c t t c a t a a a a

5340 CaMV poly(A) signal a c a a a t a c a a a t a c a t a c t a a g g g t t t c t t a t a t g c t c a a c a c a t g a g c g a a a c c c t a t a t g t t t a t g t t t a t g t a t g a t t c c c a a a g a a t a t a c g a g t t g t g t a c t c g c t t t g g g a t a t

5400 *K CaMV poly(A) signal BirA * K BirA *K g g a a c c c t a a t t c c c t t a t c t g g g a a c t a c t c a c a c a t t a t t a t g g a g a a a g c t t a t t t t c c t t g g g a t t a a g g g a a t a g a c c c t t g a t g a g t g t g t a a t a a t a c c t c t t t c g a a t a a a a

5460 320 315 310 305 EASRLSIEGGMWPKIIGDQE BirA 320 315 310 305 EASRLSIEGGMWPKIIGDQE BirA EASRLSIEGGMWPKIIGDQE t c t g c a c t a c g c a g g g a t a t t t c a c c g c c c a t c c a g g g t t t t a t t a t t c c a t c c t g c t c a a g a c g t g a t g c g t c c c t a t a a a g t g g c g g g t a g g t c c c a a a a t a a t a a g g t a g g a c g a g t

5520 300 295 290 285 LLLAGQKDIGRSIGFIEKDG BirA 300 295 290 285 LLLAGQKDIGRSIGFIEKDG BirA LLLAGQKDIGRSIGFIEKDG a g t a a t a a a g c c c c c t g t t t g t c t a t t c c g c g t g a a a t g c c a a a t a t t t c t t t a t c a c c a t c a t t a t t t c g g g g g a c a a a c a g a t a a g g c g c a c t t t a c g g t t t a t a a a g a a a t a g t g g t

5580 280 275 270 265 IILKVPRNIFNDLKEWRSLY BirA 280 275 270 265 IILKVPRNIFNDLKEWRSLY BirA IILKVPRNIFNDLKEWRSLY a t g a t a a g t t t c a c t g g g c g a t t a a t a a a a t t a t c c a g c t t t t c c c a g c g c g a c a g a t a a t a c t a t t c a a a g t g a c c c g c t a a t t a t t t t a a t a g g t c g a a a a g g g t c g c g c t g t c t a t t

5640 260 255 250 245 PALGEQEFLELAARLERILM BirA 260 255 250 245 PALGEQEFLELAARLERILM BirA PALGEQEFLELAARLERILM g g t g c c a a t c c t t c t t g t t c g a a g a g t t c c a a c g c a g c a c g t a a t t c a c g t a t t a g c a t g c c a c g g t t a g g a a g a a c a a g c t t c t c a a g g t t g c g t c g t g c a t t a a g t g c a t a a t c g t a c

5700 240 235 230 225 AALTNRDLNIGAEQLTIWGQ BirA 240 235 230 225 AALTNRDLNIGAEQLTIWGQ BirA AALTNRDLNIGAEQLTIWGQ g c c g c c a a c g t a t t a c g a t c g a g a t t g a t c c c c g c t t c c t g c a g c g t g a t c c a c c c c t g a c g g c g g t t g c a t a a t g c t a g c t c t a a c t a g g g g c g a a g g a c g t c g c a c t a g g t g g g g a c t

5760 220 215 210 205 NVVSEEVRRMAMNIGAGIVI BirA 220 215 210 205 NVVSEEVRRMAMNIGAGIVI BirA NVVSEEVRRMAMNIGAGIVI t t a a c g a c a c t c t c t t c a a c a c g g c g c a t t g c c a t g t t g a t c c c g g c t c c a a t g a c t a t t a a t t g c t g t g a g a g a a g t t g t g c c g c g t a a c g g t a c a a c t a g g g c c g a g g t t a c t g a t a a

5820 200 195 190 185 QAADGTKGTLEVLIGALKRD BirA 200 195 190 185 QAADGTKGTLEVLIGALKRD BirA QAADGTKGTLEVLIGALKRD t g c g c c g c a t c g c c a g t t t t g c c a g t c a g c t c c a c c a g a a t g c c t g c c a g c t t g c g a t c c a c g c g g c g t a g c g g t c a a a a c g g t c a g t c g a g g t g g t c t t a c g g a c g g t c g a a c g c t a g g

5880 180 175 170 165 QLYLDNPWKVRVKDAGLKRL BirA 180 175 170 165 QLYLDNPWKVRVKDAGLKRL BirA QLYLDNPWKVRVKDAGLKRL t g c a g a t a g a g g t c a t t a g g c c a t t t a a c a c g a a c t t t a t c t g c a c c c a g c t t g c g t a a t a c g t c t a t c t c c a g t a a t c c g g t a a a t t g t g c t t g a a a t a g a c g t g g g t c g a a c g c a t t a

5940 160 155 150 145 VEAMVIGIVLSLGIAAAPGQ BirA 160 155 150 145 VEAMVIGIVLSLGIAAAPGQ BirA VEAMVIGIVLSLGIAAAPGQ a c t t c c g c c a t c a c g a t a c c g a t a a c c a g a c t t a a a c c a a t c g c c g c c g c c g g g c c t t g t t g a a g g c g g t a g t g c t a t g g c t a t t g g t c t g a a t t t g g t t a g c g g c g g c g g c c c g g a a c a

6000 140 135 130 125 ELRWFMSLYLNAGFPSFWKR BirA 140 135 130 125 ELRWFMSLYLNAGFPSFWKR BirA ELRWFMSLYLNAGFPSFWKR t c c a g a c g c c a g a a c a t c g a c a a a t a t a a g t t t g c g c c a a a a g g c g a a a a c c a t t t c c g a a g g t c t g c g g t c t t g t a g c t g t t t a t a t t c a a a c g c g g t t t t c c g c t t t t g g t a a a g g c t

BstXI 6060 120 115 110 105 GRRGRGAQQYEAICADGSKL BirA 120 115 110 105 GRRGRGAQQYEAICADGSKL BirA GRRGRGAQQYEAICADGSKL c c c c g g c g a c c a c g g c c a g c c t g c t g g t a t t c t g c a a t g c a a g c a t c g c c c g a t t t a a g c g g g g c c g c t g g t g c c g g t c g g a c g a c c a t a a g a c g t t a c g t t c g t a g c g g g c t a a a t t c g

6120 100 95 90 85 EGIRDLLYQNTSDIVPLVAV BirA 100 95 90 85 EGIRDLLYQNTSDIVPLVAV BirA EGIRDLLYQNTSDIVPLVAV t c t c c g a t a c g a t c a a g a a g g t a c t g a t t c g t g g a g t c a a t c a c t g g c a g c a c g g c t a c a a g a g g c t a t g c t a g t t c t t c c a t g a c t a a g c a c c t c a g t t a g t g a c c g t c g t g c c g a t g t

6180 80 75 70 65 SGGDLQGLIQKANLLQIPEP BirA 80 75 70 65 SGGDLQGLIQKANLLQIPEP BirA SGGDLQGLIQKANLLQIPEP c t a c c g c c a t c c a g c t g a c c c a a t a t c t g t t t a g c a t t a a g t a a c t g g a t a g g c t c a g g c g a t g g c g g t a g g t c g a c t g g g t t a t a g a c a a a t c g t a a t t c a t t g a c c t a t c c g a g t c c g

DraIII 6240 60 55 50 45 LSYGKGPVTFVDVGWDRLTQ BirA 60 55 50 45 LSYGKGPVTFVDVGWDRLTQ BirA LSYGKGPVTFVDVGWDRLTQ a g g c t g t a t c c t t t a c c c g g a a c g g t a a a g a c a t c a a c g c c c c a g t c a c g c a g t g t c t g a t c c g a c a t a g g a a a t g g g c c t t g c c a t t t c t g t a g t t g c g g g g t c a g t g c g t c a c a g a c t

6300 40 35 30 25 IHKNIAARSMGLTEGLQEGS BirA 40 35 30 25 IHKNIAARSMGLTEGLQEGS BirA IHKNIAARSMGLTEGLQEGS a t g t g t t t a t t a a t a g c c g c c c g g c t c a t t c c c a g c g t t t c a c c c a a c t g c t c g c c a g a g t a c a c a a a t a a t t a t c g g c g g g c c g a g t a a g g g t c g c a a a g t g g g t t g a c g a g c g g t c t c

6360 20 15 10 5 1 HFEGNALLAILKLPVTNDKM BirA 20 15 10 5 1 HFEGNALLAILKLPVTNDKM BirA HFEGNALLAILKLPVTNDKM t g a a a t t c a c c g t t c g c t a a c a g g g c a a t c a a t t t c a g t g g c a c g g t g t t a t c c t t c a t c a c t t t a a g t g g c a a g c g a t t g t c c c g t t a g t t a a a g t c a c c g t g c c a c a a t a g g a a g t a g

6420 ELDCVIPQIDDAEAM t c g a g g t c a c a c a c g a t t g g t t g a a t a t c a t c a g c c t c a g c c a t t t t t t a t g a g c t g c a a a g c t c c a g t g t g t g c t a a c c a a c t t a t a g t a g t c g g a g t c g g t a a a a a a t a c t c g a c g t t

6480 a c a c a c a a a a a g a g t t c a a t a c a g t c a a a t a a a c c c t g a t t g a a t c a g a a a t a g c t t t c t t g t g t g t t t t t c t c a a g t t a t g t c a g t t t a t t t g g g a c t a a c t t a g t c t t t a t c g a a a g a

6540 g t t c a a c g t a c g a c a c t a c a a a t c c t a a a g t t t c g a a g a a t c t a t a c a c a a a c t t c a t c t c a a g t t g c a t g c t g t g a t g t t t a g g a t t t c a a a g c t t c t t a g a t a t g t g t t t g a a g t a g a

6600 a a c c t t a a a c a g t g t t c a g a t t c a a t c t a a c a t t g a c a g t g t t c a g a t t c a a t c t a a c t t t t g g a a t t t g t c a c a a g t c t a a g t t a g a t t g t a a c t g t c a c a a g t c t a a g t t a g a t t g a a

6660 c a a c a g t t c a g a t t t c a a t t c a c a t a t a c a a a t c a t t t c c c a g g t a a c a t g a a c a t g g a t g t t g t c a a g t c t a a a g t t a a g t g t a t a t g t t t a g t a a a g g g t c c a t t g t a c t t g t a c c t a

6720 c t c t c c a t c a a g g t c a a g c c a a a t t c t g g t a g c t a t a a t c g a g c t a a c t g a t c g g t c a a c g a g a g g t a g t t c c a g t t c g g t t t a a g a c c a t c g a t a t t a g c t c g a t t g a c t a g c c a g t t g

6780 t t a g a t c g a t t c t a g t a a a a c c a a a a g a t t t a g t g g a g g t t c a c a g a t c c a a a t t a a a c g a a t c t a g c t a a g a t c a t t t t g g t t t t c t a a a t c a c c t c c a a g t g t c t a g g t t t a a t t t g c

6840 a t a t c c a g a t c t c a a g g a a a t t g a g a a a a c a a g a t c g a g a t c c a g c a a a t a a a g t a g a t c t a t a g g t c t a g a g t t c c t t t a a c t c t t t t g t t c t a g c t c t a g g t c g t t t a t t t c a t c t a g

6900 c a g a a a g t t c c t a t t a c c t t g g a a a g a a a g a g c g g a a g a a g a t g a g a t t g a g g a a g a t t c g t c t t t c a a g g a t a a t g g a a c c t t t c t t t c t c g c c t t c t t c t a c t c t a a c t c c t t c t a a g

6960 a a a a c g g a g a a t g a a t c t c c t g g a t t a t c t c t t a c t t t c t c t c t t a g t c t t c t t c c t t g t t t t t g c c t c t t a c t t a g a g g a c c t a a t a g a g a a t g a a a g a g a g a a t c a g a a g a a g g a a c a

7020 t c t t c t c t g t c a a g t c g c c g g a g a t t c a a a a c g g c t g a t g a a a g t g a g g a g g a c a a c g a g a g a a g a g a c a g t t c a g c g g c c t c t a a g t t t t g c c g a c t a c t t t c a c t c c t c c t g t t g c t c

7080 a c a a t t c a a a g c g g a g a g g a a a a t a t a t g a a t t t a t a t a g g c g g g t t t a t c t c t t a c a a c t g t t a a g t t t c g c c t c t c c t t t t a t a t a c t t a a a t a t a t c c g c c c a a a t a g a g a a t g t t g

7140 t t t a t t t t c g g c c t t t c a a a a a a a t a a t t a a a a t c g a c a g a c a c g a a t c a t t t c g a c c a c a a a t a a a a g c c g g a a a g t t t t t t t a t t a a t t t t a g c t g t c t g t g c t t a g t a a a g c t g g t g

7200 a g g t a a a g a t a a c g t g a c c t g g c t g t c a g a c a g c c t t t t c c c t c g t g t t a a c t a a t t t t t t c c a t t t c t a t t g c a c t g g a c c g a c a g t c t g t c g g a a a a g g g a g c a c a a t t g a t t a a a a a

7260 a a a c t a a t t a a t c a t c t c a g c c c t t g g a t t a g t t c t t t t g c t t t g a t g g c t t c a t g a c t g t t t g a t t a a t t a g t a g a g t c g g g a a c c t a a t c a a g a a a a c g a a a c t a c c g a a g t a c t g a c

7320 t g a c c t g c t c g a t c c g c g t g t t a c a t g a c a g c t c c g t t t t t t t a g t g g t t a a c t t a a a c c a c t g g a c g a g c t a g g c g c a c a a t g t a c t g t c g a g g c a a a a a a a t c a c c a a t t g a a t t t g g

7380 g a g t c a a t c c a g g c a a c g t t a g t c g t c g t c g t g g t t g g c t t g t t c a a t t a g a t t t c a t a c c t c a g t t a g g t c c g t t g c a a t c a g c a g c a g c a c c a a c c g a a c a a g t t a a t c t a a a g t a t g

7440 a a t t c a a c g t a a t t t a a t t c g t t t t c t a t t a g a a t t g t a t c a t a a t t a a t t c a g a c c g t g t t a a g t t g c a t t a a a t t a a g c a a a a g a t a a t c t t a a c a t a g t a t t a a t t a a g t c t g g c a c

7500 a a a g a a a g t g t c t t t c a t g a t g t g t t t a t g g a t a t t t a t a c a a t a a g a t a c a a t g t t t c a t t t c t t t c a c a g a a a g t a c t a c a c a a a t a c c t a t a a a t a t g t t a t t c t a t g t t a c a a a g t

7560 t c a t a t t c a c t a t t c a c g a t t a g t a t g t a c a t t a a a t a a t g g c t a c t a c t a c a t c c g a a c a g t a t a a g t g a t a a g t g c t a a t c a t a c a t g t a a t t t a t t a c c g a t g a t g a t g t a g g c t t g

7620 t c g t c a a a a c g a t t c t g a a t c a a t t a t a c a t a t g c t g a c t c t t g c a t a c a t a a a a a a t a g a g c a g t t t t g c t a a g a c t t a g t t a a t a t g t a t a c g a c t g a g a a c g t a t g t a t t t t t t a t c

7680 t t g t t t a a a t t t t g t c t a a c t a a t g t t t g g t a t a a g t a t a a t g t t g a g t t g a g a t a c c a a a a c a a a t t t a a a a c a g a t t g a t t a c a a a c c a t a t t c a t a t t a c a a c t c a a c t c t a t g g t t

7740 t t a c a t c g a g t c t a g c c a t t t t g t c g t g c c a t a t t c g t c a a a a c t t t c t t a c a t a a t g a t a a t g t a g c t c a g a t c g g t a a a a c a g c a c g g t a t a a g c a g t t t t g a a a g a a t g t a t t a c t a

7800 a a c c t a g a t c t a g a t g a g a t a t g t a t c a a t g t a t t t g a g a t c a t a a t t a a g t t c g t t c t a t t g g a t c t a g a t c t a c t c t a t a c a t a g t t a c a t a a a c t c t a g t a t t a a t t c a a g c a a g a t

7860 OCS terminator a a t t t t g t c c c t g c a g g t c c t g c t g a g c c t c g a c a t g t t g t c g c a a a a t t c g c c c t g g a c t t a a a a c a g g g a c g t c c a g g a c g a c t c g g a g c t g t a c a a c a g c g t t t t a a g c g g g a c c t g

7920 OCS terminator c c g c c c a a c g a t t t g t c g t c a c t g t c a a g g t t t g a c c t g c a c t t c a t t t g g g g c c c a c a t g g c g g g t t g c t a a a c a g c a g t g a c a g t t c c a a a c t g g a c g t g a a g t a a a c c c c g g g t g t a

BstEII 7980 OCS terminator a c a c c a a a a a a a t g c t g c a t a a t t c t c g g g g c a g c a a g t c g g t t a c c c g g c c g c c g t g c t t g t g g t t t t t t t a c g a c g t a t t a a g a g c c c c g t c g t t c a g c c a a t g g g c c g g c g g c a c g a

8040 OCS terminator g g a c c g g g t t g a a t g g t g c c c g t a a c t t t c g g t a g a g c g g a c g g c c a a t a c t c a a c t t c a c c t g g c c c a a c t t a c c a c g g g c a t t g a a a g c c a t c t c g c c t g c c g g t t a t g a g t t g a a g t

8100 OCS terminator a g g a a t c t c a c c c a t g c g c g c c g g c g g g g a a c c g g a g t t c c c t t c a g t g a g c g t t a t t a g t c c t t a g a g t g g g t a c g c g c g g c c g c c c c t t g g c c t c a a g g g a a g t c a c t c g c a a t a a t c

8160 OCS terminator t t c g c c g c t c g g t g t g t c g t a g a t a c t a g c c c c t g g g g c a c t t t t g a a a t t t g a a t a a g a a a g c g g c g a g c c a c a c a g c a t c t a t g a t c g g g g a c c c c g t g a a a a c t t t a a a c t t a t t c t

8220 OCS terminator t t t a t g t a a t c a g t c t t t t a g g t t t g a c c g g t t c t g c c g c t t t t t t t a a a a t t g g a t t t g a a a t a c a t t a g t c a g a a a a t c c a a a c t g g c c a a g a c g g c g a a a a a a a t t t t a a c c t a a a c

MfeI 8280 OCS terminator t a a t a a t a a a a c g c a a t t g t t t g t t a t t g t g g c g c t c t a t c a t a g a t g t c g c t a t a a a c c a t t a t t a t t t t g c g t t a a c a a a c a a t a a c a c c g c g a g a t a g t a t c t a c a g c g a t a t t t g g

8340 OCS terminator t a t t c a g c a c a a t a t a t t g t t t t c a t t t t a a t a t t g t a c a t a t a a g t a g t a g g g t a c a a t a t a a g t c g t g t t a t a t a a c a a a a g t a a a a t t a t a a c a t g t a t a t t c a t c a t c c c a t g t t a

8400 OCS terminator c a g t a a a t t g a a c g g a g a a t a t t a t t c a t a a a a a t a c g a t a g t a a c g g g t g a t a t a t t c a g t c a t t t a a c t t g c c t c t t a t a a t a a g t a t t t t t a t g c t a t c a t t g c c c a c t a t a t a a g t

8460 OCS terminator t t a g a a t g a a c c g a a a c c g g c g g t a a g g a t c t g a g c t a c a c a t g c t c a g g t t t t t t a c a a a a t c t t a c t t g g c t t t g g c c g c c a t t c c t a g a c t c g a t g t g t a c g a g t c c a a a a a a t g t t

8520 OCS terminator c g t g c a c a a c a g a a t t g a a a g c a a a t a t c a t g c g a t c a t a g g c g t c t c g c a t a t c t c a t t g c a c g t g t t g t c t t a a c t t t c g t t t a t a g t a c g c t a g t a t c c g c a g a g c g t a t a g a g t a a

8580 OCS terminator *LRALS a a a g c a g g a c t c t a g g g a c t a g t c c c g g g t c t t a a t t a a c t c t c t a g a g g c g c g c c a a g c t t t c g t c c t g a g a t c c c t g a t c a g g g c c c a g a a t t a a t t g a g a g a t c t c c g c g c g g t t c g

8640 (in frame with AviTagâ„¢) *SGGTD 15 10 5 EHWEIKQAEFI AviTagâ„¢ RSSLDVLIRMGNLRRLKSSI t t c t a c t a c t a a g a t c c a c c a g t a t c c t c a t g c c a t t c a a t c t t c t g a g c t t c g a a g a t a a a g a t g a t g a t t c t a g g t g g t c a t a g g a g t a c g g t a a g t t a g a a g a c t c g a a g c t t c t a t

PspXI 8700 (in frame with HRV 3C site) GAMSGELGTHTRQHET 1 DNLG AviTagâ„¢ TLDVLWLDRSDPTLGKISPS t c g t t a a g t c c a c c a g c c a t a g a t c c c t c g a g t c c g g t g t g a g t c c t t t g a t g c t c g g t g a g c a a t t c a g g t g g t c g g t a t c t a g g g a g c t c a g g c c a c a c t c a g g a a a c t a c g a g c c a c

8760 (in frame with HRV 3C site) LKDSRSFSKGCEPCKYPKEG SIVGLSLNEVSLANILNKEL a g c t t a t c a c t c c t a g a g a a a g a t t t t c c a c a c t c a g g g c a t t t a t a a g g t t t t t c t c c a t c g a a t a g t g a g g a t c t c t t t c t a a a a g g t g t g a g t c c c g t a a a t a t t c c a a a a a g a g g t

Bsu36I 8820 (in frame with HRV 3C site) THTRQHARLNARQSFSKGCE THGKILGLTQERLSHNEVSL g t g t g t g t c c t t t g a t g a g c c c t a a g g t t t g c t c t c t g a g a g a a t g a t t t t c c a c a c t c a c a c a c a c a g g a a a c t a c t c g g g a t t c c a a a c g a g a g a c t c t c t t a c t a a a a g g t g t g a g t

8880 (in frame with HRV 3C site) PCKYPKEGTHTRQHRVLNGS ANILNKVLIPGKTGLLTELH g g g c a t t t a t a a g g t t t t t c a c c a g t a t g g g t c c t t t g g t g c c t a a c a a g g t t t c c a g a t c c c g t a a a t a t t c c a a a a a g t g g t c a t a c c c a g g a a a c c a c g g a t t g t t c c a a a g g t c t a

8940 (in frame with HRV 3C site) TSFSKGCEPCKYPKEGTHTR QSHNEVSLANILNKEPILGR g t t g a g a a t g a t t t t c c a c a c t c a g g g c a t t t a t a a g g t t t t t c t c c g g t a t g a g t c c t c c a a c t c t t a c t a a a a g g t g t g a g t c c c g t a a a t a t t c c a a a a a g a g g c c a t a c t c a g g a g

9000 (in frame with HRV 3C site) QHRVLSSSQSFSKGCEPCKY IGLLLHLNHSHNEVSLANIL t g a t g c c t a a c a a g a g a t g a a g a t t g t g a g a a t g a t t t t c c a c a c t c a g g g c a t t t a t a a a c t a c g g a t t g t t c t c t a c t t c t a a c a c t c t t a c t a a a a g g t g t g a g t c c c g t a a a t a t t

9060 (in frame with HRV 3C site) PKEGTHTRQHTTLHDSRSFS NKVLIPGRIPPLIIVGVSHN g g t t t t t c a c c a g t a t g g g t c c t c t g a t g g g t g g t a a g a t g a t c a c t c c t a c t g a a t g a t c c a a a a a g t g g t c a t a c c c a g g a g a c t a c c c a c c a t t c t a c t a g t g a g g a t g a c t t a c t a

9120 (in frame with HRV 3C site) KGCEPCKYPKEGTHTRQHRR EVSLANILNKVPIPGKTGGL t t t c c a c a c t c a g g g c a t t t a t a a g g t t t t t c a c c g g t a t g g g t c c t t t g g t g c c t c c t a a a a g g t g t g a g t c c c g t a a a t a t t c c a a a a a g t g g c c a t a c c c a g g a a a c c a c g g a g g a t

9180 (in frame with HRV 3C site) LDGSQSFSKGCEPCKYPEL P HRV 3C site IELNLSLNEVSLANILSSLD a g a t c t c c a g a t t g a g a g a a a g a t t t t c c a c a c t c a g g g c a t t t a t a a g g c t c g a g a g g t t c t a g a g g t c t a a c t c t c t t t c t a a a a g g t g t g a g t c c c g t a a a t a t t c c g a g c t c t c c a

BamHI 9240 5 1 GQFLVEL SG 20 15 KDDDDKYDKDD HRV 3C site 3xFLAG KSFFRLPDKTTMTKTTKTTM c c t t g g a a a a g a a c c t c a a g g g a t c c t t t g t c g t c a t c g t c t t t g t a g t c t t t g t c g t c a g g a a c c t t t t c t t g g a g t t c c c t a g g a a a c a g c a g t a g c a g a a a c a t c a g a a a c a g c a g t

9300 10 5 1 DDKYDKDDDDKYD 3xFLAG TKTTKTTM t c g t c t t t g t a g t c t t t g t c g t c a t c g t c t t t g t a g t c g g t a c c a a g c t t g g c g c g c c c g a g c a g a a a c a t c a g a a a c a g c a g t a g c a g a a a c a t c a g c c a t g g t t c g a a c c g c g c g g g c

9360 attB2 t c c c g g g t c t t a a t t a a c t c t c t a g a c t c a c c t a g c a c c a c t t t g t a c a a g a a a g c t g g g a g g g c c c a g a a t t a a t t g a g a g a t c t g a g t g g a t c g t g g t g a a a c a t g t t c t t t c g a c c c

9420 attB2 t c g g c g c g c c c a c c c t t c t t g g c a g c a g c g t g c t t g g c c t t g t t g t g a g a c t c a a g t g c a a g c c g c g c g g g t g g g a a g a a c c g t c g t c g c a c g a a c c g g a a c a a c a c t c t g a g t t c a c g t

9480 t t c c c t g a g t t g a a a g t c c t g c c a a a g a g a t a t a a g c c a a c a a g c g a a a t g t t a t a a c a a a a g g g a c t c a a c t t t c a g g a c g g t t t c t c t a t a t t c g g t t g t t c g c t t t a c a a t a t t g t t

9540 a c a g a a a t t t t g a g c a g t g a a g a c a a g a g t t a g a g a a a g a g a g a g a g t c t c a c t t c t t g c t g t c t t t a a a a c t c g t c a c t t c t g t t c t c a a t c t c t t t c t c t c t c t c a g a g t g a a g a a c g

9600 a t g a a c c a c a t g a g a c t t g a c t g g c c g a c t t t g g g c t c t g g t t t g c a g c c t t t c c c c c t t t a c t t g g t g t a c t c t g a a c t g a c c g g c t g a a a c c c g a g a c c a a a c g t c g g a a a g g g g g a a

9660 t c t t c t t c t c a t c t g t g c c a a a g a a g a c g a t g c a g g t t c a a a t g a a t a c t c g t g t t t c c c a g a a g a a g a g t a g a c a c g g t t t c t t c t g c t a c g t c c a a g t t t a c t t a t g a g c a c a a a g g g

9720 a a t t t a g t g a a t a a t g t a a g a g a a t g g t t t g t a t c t a t a c c t g t t t t c t g a g g a g t a a c t t t a a a t c a c t t a t t a c a t t c t c t t a c c a a a c a t a g a t a t g g a c a a a a g a c t c c t c a t t g a

9780 g c t a c t t t c g c c t t c t t t g c t g a c a c a g g t g c t t t a g g g g t a g t t t c a t t a g c t c t c t t c c g a t g a a a g c g g a a g a a a c g a c t g t g t c c a c g a a a t c c c c a t c a a a g t a a t c g a g a g a a g

9840 t t g c t t g a t g c a g g c t t c t t a g g t g t a g g c t c t t c t t c c t c a g a a t c c t c a c c a t c a g a a a a c g a a c t a c g t c c g a a g a a t c c a c a t c c g a g a a g a a g g a g t c t t a g g a g t g g t a g t c t t

9900 t c a t c t t c a t c c a t c t g c a a g a a t c a t a a c a c a a g a a t g c g t c a c c a a c a t t t g t t c a a a a g t a g a a g t a g g t a g a c g t t c t t a g t a t t g t g t t c t t a c g c a g t g g t t g t a a a c a a g t t t

9960 a a a g a t g t c t c a t c t c c t c a g t c a t t a t t c t g a a a t g g t a a a c a a a a a g a g a c a a a t c a c t t t c t a c a g a g t a g a g g a g t c a g t a a t a a g a c t t t a c c a t t t g t t t t t c t c t g t t t a g t g

10,020 a g a a a g c t a t a a c a a c t c a c t c c g t c a g a a t c a g a c t c a t c c t c t t c a t c a t c a a c a g c t t c t t t c g a t a t t g t t g a g t g a g g c a g t c t t a g t c t g a g t a g g a g a a g t a g t a g t t g t c g a

10,080 g g c t t c a c t t c t g c a g g c t t a g c c t t t g g t t t a g c t a c a g c c t t g g c a g c a t t c c c t g c a c c g a a g t g a a g a c g t c c g a a t c g g a a a c c a a a t c g a t g t c g g a a c c g t c g t a a g g g a c g t

10,140 a a t g g a a c t a g t t a t t a c c a a t t a t c a g g a t c a c a a a t c a a g t a g t c t c t g t c t a g t t t a t t a c c t t g a t c a a t a a t g g t t a a t a g t c c t a g t g t t t a g t t c a t c a g a g a c a g a t c a a a t

10,200 a g t a c c a g c a g g a a c t t c t t c c t c t t c t t c c t c t t c t t c c t c t g a a t a t a t c a a t a c a a c t c a t g g t c g t c c t t g a a g a a g g a g a a g a a g g a g a a g a a g g a g a c t t a t a t a g t t a t g t t g

10,260 a c a t a a a c g a a t c a t c a g c a a c c a a g c c a a t a t t a c a g t t a c a a a c a g a a t a a t t g a a t t t g t a t t t g c t t a g t a g t c g t t g g t t c g g t t a t a a t g t c a a t g t t t g t c t t a t t a a c t t a a

10,320 g a a a c a g a t t t t a t c t t g c a a a c t c a c c a g a a t a g c c t t g t g g c t c a a t g t t g g g a g t t t c t t t g t c t a a a a t a g a a c g t t t g a g t g g t c t t a t c g g a a c a c c g a g t t a c a a c c c t c a a a

10,380 t g t a t c c a a c a a a g t a a a c a c t t c c t t t t c c c c a a g t g t g a g a a a g c t c a a a c t c c t t g t a c a t a g g t t g t t t c a t t t g t g a a g g a a a a g g g g t t c a c a c t c t t t c g a g t t t g a g g a a c a

10,440 c g a a t a c c a a a t c a c a g a a a a g c t g a g g g a t g t t c t c a g t c g a t a g a g t t c c c a g a a c c a g c t t a t g g t t t a g t g t c t t t t c g a c t c c c t a c a a g a g t c a g c t a t c t c a a g g g t c t t g g t

10,500 a g t t c t g g t t c c c a a c c t t t a c a t g t a a a g g c a c a a a c t c t c c c t t c t t g t t t t t a c a t t t c a a g a c c a a g g g t t g g a a a t g t a c a t t t c c g t g t t t g a g a g g g a a g a a c a a a a a t g t a a

10,560 c t c c a a g c g a t g c c t a c a c a g a t a a a c a a t c c a a g a t t g t t t a c a c t a g a c c t c t a a c a a g a g g t t c g c t a c g g a t g t g t c t a t t t g t t a g g t t c t a a c a a a t g t g a t c t g g a g a t t g t t

10,620 c t c t c a g a a t c a a a a t c a a t a a g a t t t g a t a c t a a a c c t g a g a a a c g t g g a t a a g a a t g c g a g a g t c t t a g t t t t a g t t a t t c t a a a c t a t g a t t t g g a c t c t t t g c a c c t a t t c t t a c g

10,680 c t t c t t c a g g a g t c a c t g t a a c t g g c t t t c c t g a t t t a a c t t c a a t t c c t g a t t a c a t c g g a a g a a g t c c t c a g t g a c a t t g a c c g a a a g g a c t a a a t t g a a g t t a a g g a c t a a t g t a g c

10,740 t t t t a a c a a a a a c c a a c a t g a g t a t c a a g a t a c a g c t t c a a g t t a t a c a c t a c c a c a c t a a a a a t t g t t t t t g g t t g t a c t c a t a g t t c t a t g t c g a a g t t c a a t a t g t g a t g g t g t g a t

10,800 a t t c c t a t a t a a t a t a a c c a t t t c t a t g t g a g a a t a t t g c a a c t t a a a a c c c t a g a t g a a t a a g g a t a t a t t a t a t t g g t a a a g a t a c a c t c t t a t a a c g t t g a a t t t t g g g a t c t a c t t

10,860 g c t a c a c a a c t a c a c c a c a c a a g g a a g c a g c c a c a a g a g a g t t c g a a a a c a g a t t t t a a g c g a t g t g t t g a t g t g g t g t g t t c c t t c g t c g g t g t t c t c t c a a g c t t t t g t c t a a a a t t c

10,920 a a a a a c c c a a a a a g c t t c a g a c a a a a c a g a g a a g a t t c t t a c c c c a g a a c t c c a t g g c t a t t t t t g g g t t t t t c g a a g t c t g t t t t g t c t c t t c t a a g a a t g g g g t c t t g a g g t a c c g a t

10,980 a g a a t c a a g g t t g a g a a a a a g a g a g g a t t t t t a g g g t t t a t g a g c a g c t g a a a a t g t a a g t c t t a g t t c c a a c t c t t t t t c t c t c c t a a a a a t c c c a a a t a c t c g t c g a c t t t t a c a t t c

11,040 a g a a g a a g a a a g g g t t t t a a g a g a t t a t t t a t a a g a c a g a g g g t t t t a a t a c g a a g c c g t t c t t c t t c t t t c c c a a a a t t c t c t a a t a a a t a t t c t g t c t c c c a a a a t t a t g c t t c g g c a

11,100 t t t a g g g t t t t a a t a c g a c g c c g t t a g g g t c t t t a c t c t t t a g g g t t t t t c t t c a g t a c t a a a t c c c a a a a t t a t g c t g c g g c a a t c c c a g a a a t g a g a a a t c c c a a a a a g a a g t c a t g a

ZraIAatII 11,160 a a c g a c g t c g t t a a a g a t a t g a a a g t t t g t g a a a a a a c a a a a t g c t c c g a t c a a a t t c a a t t g c t g c a g c a a t t t c t a t a c t t t c a a a c a c t t t t t t g t t t t a c g a g g c t a g t t t a a g t t

11,220 a c g g c g g a a a t c a g t a t a g g c g a a a t c t t c c t c c g t c g a a g c t c a g g g g a a t g t c t t c a a t g c c g c c t t t a g t c a t a t c c g c t t t a g a a g g a g g c a g c t t c g a g t c c c c t t a c a g a a g t t

11,280 g c t c t c a a g g c t t c a c g a t t g a t g c a g c c a t g g a g c t g c t g a t t t c g a c a a a g g t g a a g g c g a g a g t t c c g a a g t g c t a a c t a c g t c g g t a c c t c g a c g a c t a a a g c t g t t t c c a c t t c c

11,340 attB1 g g g c g g c c g c g g a g c c t g c t t t t t t g t a c a a a c t t g t t g a t g a t c c c g g g c c c g c g g t a c c c c g c c g g c g c c t c g g a c g a a a a a a c a t g t t t g a a c a a c t a c t a g g g c c c g g g c g c c a t g

EcoRI 11,400 MAS promoter c g t c g a c t g c a g a a t t c a a t t c t g a g a t t t t t c a a a t c a g t g c g c a a g a c g t g a c g t a a g g c a g c t g a c g t c t t a a g t t a a g a c t c t a a a a a g t t t a g t c a c g c g t t c t g c a c t g c a t t c

11,460 MAS promoter t a t c c g a g t c a g t t t t t a t t t t t c t a c t a a t t t g g t c g t t t a t t t c g g c g t g t a g g a c a t a t a g g c t c a g t c a a a a a t a a a a a g a t g a t t a a a c c a g c a a a t a a a g c c g c a c a t c c t g t a

11,520 MAS promoter g g c a a c c g g g c c t g a a t t t c g c g g g t a t t c t g t t t c t a t t c c a a c t t t t t c t t g a t c c g c c c g t t g g c c c g g a c t t a a a g c g c c c a t a a g a c a a a g a t a a g g t t g a a a a a g a a c t a g g c g

11,580 MAS promoter a g c c a t t a a c g a c t t t t g a a t a g a t a c g c t g a c a c g c c a a g c c t c g c t a g t c a a a a g t g t t c g g t a a t t g c t g a a a a c t t a t c t a t g c g a c t g t g c g g t t c g g a g c g a t c a g t t t t c a c a

11,640 MAS promoter a c c a a a c a a c g c t t t a c a g c a a g a a c g g a a t g c g c g t g a c g c t c g c g g t g a c g c c a t t t c t g g t t t g t t g c g a a a t g t c g t t c t t g c c t t a c g c g c a c t g c g a g c g c c a c t g c g g t a a a g

11,700 MAS promoter g c c t t t t c a g a a a t g g a t a a a t a g c c t t g c t t c c t a t t a t a t c t t c c c a a a t t a c c a a t a c g g a a a a g t c t t t a c c t a t t t a t c g g a a c g a a g g a t a a t a t a g a a g g g t t t a a t g g t t a t

11,760 1 MS MAS promoter BlpR MS *LWDRYVLDFRHA c a t t a c a c t a g c a t c t g a a t t t c a t a a c c a a t c t c g a t a c a c c a a a t c g a a t c g a t g a g c g t a a t g t g a t c g t a g a c t t a a a g t a t t g g t t a g a g c t a t g t g g t t t a g c t t a g c t a c t c g

11,820 5 10 15 20 PERRPADIRRATEADMPAVC BlpR PERRPADIRRATEADMPAVC WFSARGVDATGGLRVHRRDA c c a g a a c g a c g c c c g g c c g a c a t c c g c c g t g c c a c c g a g g c g g a c a t g c c g g c g g t c t g c g g t c t t g c t g c g g g c c g g c t g t a g g c g g c a c g g t g g c t c c g c c t g t a c g g c c g c c a g a c g

11,880 25 30 35 40 TIVNHYIETSTVNFRTEPQE BlpR TIVNHYIETSTVNFRTEPQE GDDVVVDLCARDVETGLRLF a c c a t c g t c a a c c a c t a c a t c g a g a c a a g c a c g g t c a a c t t c c g t a c c g a g c c g c a g g a a t g g t a g c a g t t g g t g a t g t a g c t c t g t t c g t g c c a g t t g a a g g c a t g g c t c g g c g t c c t t

11,940 45 50 55 60 PQEWTDDLVRLRERYPWLVA BlpR PQEWTDDLVRLRERYPWLVA RLLPRVVEDTQPLAIGPEDG c c g c a g g a g t g g a c g g a c g a c c t c g t c c g t c t g c g g g a g c g c t a t c c c t g g c t c g t c g c c g g c g t c c t c a c c t g c c t g c t g g a g c a g g c a g a c g c c c t c g c g a t a g g g a c c g a g c a g c g g

12,000 65 70 75 80 EVDGEVAGIAYAGPWKARNA BlpR EVDGEVAGIAYAGPWKARNA LHVALDGADGVRAGPLCAVG g a g g t g g a c g g c g a g g t c g c c g g c a t c g c c t a c g c g g g c c c c t g g a a g g c a c g c a a c g c c c t c c a c c t g c c g c t c c a g c g g c c g t a g c g g a t g c g c c c g g g g a c c t t c c g t g c g t t g c g g

12,060 85 90 95 100 YDWTAESTVYVSPRHQRTGL BlpR YDWTAESTVYVSPRHQRTGL VVPRGLRGHVDGGAVLPRSQ t a c g a c t g g a c g g c c g a g t c g a c c g t g t a c g t c t c c c c c c g c c a c c a g c g g a c g g g a c t g a t g c t g a c c t g c c g g c t c a g c t g g c a c a t g c a g a g g g g g g c g g t g g t c g c c t g c c c t g a c

PaqCI 12,120 105 110 115 120 GSTLYTHLLKSLEAQGFKSV BlpR GSTLYTHLLKSLEAQGFKSV AGREVGVQQLGQLCLAELAH g g c t c c a c g c t c t a c a c c c a c c t g c t g a a g t c c c t g g a g g c a c a g g g c t t c a a g a g c g t g c c g a g g t g c g a g a t g t g g g t g g a c g a c t t c a g g g a c c t c c g t g t c c c g a a g t t c t c g c a c

FspAI 12,180 125 130 135 140 VAVIGLPNDPSVRMHEALGY BlpR VAVIGLPNDPSVRMHEALGY DSDDPQGVVRAHAHVLRESI g t c g c t g t c a t c g g g c t g c c c a a c g a c c c g a g c g t g c g c a t g c a c g a g g c g c t c g g a t a t c a g c g a c a g t a g c c c g a c g g g t t g c t g g g c t c g c a c g c g t a c g t g c t c c g c g a g c c t a t a

12,240 145 150 155 160 APRGMLRAAGFKHGNWHDVG BlpR APRGMLRAAGFKHGNWHDVG GGAAHQPRGAELVPVPM g c c c c c c g c g g c a t g c t g c g g g c g g c c g g c t t c a a g c a c g g g a a c t g g c a t g a c g t g g g t c g g g g g g c g c c g t a c g a c g c c c g c c g g c c g a a g t t c g t g c c c t t g a c c g t a c t g c a c c c a

12,300 165 170 175 180 FWQLDFSLPVPPRPVLPVTE BlpR FWQLDFSLPVPPRPVLPVTE t t c t g g c a g c t g g a c t t c a g c c t g c c g g t a c c g c c c c g t c c g g t c c t g c c c g t c a c c g a g a a g a c c g t c g a c c t g a a g t c g g a c g g c c a t g g c g g g g c a g g c c a g g a c g g g c a g t g g c t c

12,360 I* BlpR MAS terminator I*M a t t t g a c t g c a g a a t c t t g g a c t c c c a t g t t g g c a a a g g c a a c c a a a c a a a c a a t g a a t g t a a a c t g a c g t c t t a g a a c c t g a g g g t a c a a c c g t t t c c g t t g g t t t g t t t g t t a c t t a c

12,420 MAS terminator IRSCIWGGLSISTAIWAELK a t c c g c t c c t g c a t a t g g g g c g g t t t g a g t a t t t c a a c t g c c a t t t g g g c t g a a t t g a a g t a g g c g a g g a c g t a t a c c c c g c c a a a c t c a t a a a g t t g a c g g t a a a c c c g a c t t a a c t t c

12,480 MAS terminator TCSCQKFRDLTQYSVISANI a c a t g c t c c t g t c a g a a a t t c c g t g a t c t t a c t c a a t a t t c a g t a a t c t c g g c c a a t a t c t g t a c g a g g a c a g t c t t t a a g g c a c t a g a a t g a g t t a t a a g t c a t t a g a g c c g g t t a t a g

12,540 MAS terminator LNVRGFICLCIVSSIHVTFA c t a a a t g t g c g t g g c t t t a t c t g t c t t t g t a t t g t t t c a t c a a t t c a t g t a a c g t t t g c t g a t t t a c a c g c a c c g a a a t a g a c a g a a a c a t a a c a a a g t a g t t a a g t a c a t t g c a a a c g a

Eco53kISacI 12,600 MAS terminator FLMNFQINYQIRARINSALI t t t c t t a t g a a t t t t c a a a t a a a t t a t c a g a t c c g a g c t c g a a t t a a t t c g g c g t t a a t t a a a g a a t a c t t a a a a g t t t a t t t a a t a g t c t a g g c t c g a g c t t a a t t a a g c c g c a a t t a a

12,660 LB T-DNA repeat QYIKNVRNVLLSCLSVNLFT c a g t a c a t t a a a a a c g t c c g c a a t g t g t t a t t a a g t t g t c t a a g c g t c a a t t t g t t t a c a g t c a t g t a a t t t t t g c a g g c g t t a c a c a a t a a t t c a a c a g a t t c g c a g t t a a a c a a a t g t

12,720 LB T-DNA repeat PQYILPPASQQLPDRQLGTK c c a c a a t a t a t c c t g c c a c c a g c c a g c c a a c a g c t c c c c g a c c g g c a g c t c g g c a c a a a a g g t g t t a t a t a g g a c g g t g g t c g g t c g g t t g t c g a g g g g c t g g c c g t c g a g c c g t g t t t t

12,780 SPLDTGSPSVRDGVSGRAVV t c a c c a c t c g a t a c a g g c a g c c c a t c a g t c c g g g a c g g c g t c a g c g g g a g a g c c g t t g t a a g t g g t g a g c t a t g t c c g t c g g g t a g t c a g g c c c t g c c g c a g t c g c c c t c t c g g c a a c a t

12,840 RRQTLLMLPMLFGRTATKLP a g g c g g c a g a c t t t g c t c a t g t t a c c g a t g c t a t t c g g a a g a a c g g c a a c t a a g c t g c c g t c c g c c g t c t g a a a c g a g t a c a a t g g c t a c g a t a a g c c t t c t t g c c g t t g a t t c g a c g g c

12,900 GLKHG* g g t t t g a a a c a c g g a t g a t c t c g c g g a g g g t a g c a t g t t g a t t g t a a c g a t g a c a g a g c g c c a a a c t t t g t g c c t a c t a g a g c g c c t c c c a t c g t a c a a c t a a c a t t g c t a c t g t c t c g c

12,960 t t g c t g c c t g t g a t c a c c g c g g t t t c a a a a t c g g c t c c g t c g a t a c t a t g t t a t a c g c c a a a c g a c g g a c a c t a g t g g c g c c a a a g t t t t a g c c g a g g c a g c t a t g a t a c a a t a t g c g g t

13,020 a c t t t g a a a a c a a c t t t g a a a a a g c t g t t t t c t g g t a t t t a a g g t t t t a g a a t g c a a g g a t g a a a c t t t t g t t g a a a c t t t t t c g a c a a a a g a c c a t a a a t t c c a a a a t c t t a c g t t c c t

13,080 a c a g t g a a t t g g a g t t c g t c t t g t t a t a a t t a g c t t c t t g g g g t a t c t t t a a a t a c t g t a t g t c a c t t a a c c t c a a g c a g a a c a a t a t t a a t c g a a g a a c c c c a t a g a a a t t t a t g a c a t

13,140 1 5 10 MAKMRISPELKKL KanR MAKMRISPELKKL g a a a a g a g g a a g g a a a t a a t a a a t g g c t a a a a t g a g a a t a t c a c c g g a a t t g a a a a a a c t c t t t t c t c c t t c c t t t a t t a t t t a c c g a t t t t a c t c t t a t a g t g g c c t t a a c t t t t t t g a

13,200 15 20 25 30 IEKYRCVKDTEGMSPAKVYK KanR IEKYRCVKDTEGMSPAKVYK g a t c g a a a a a t a c c g c t g c g t a a a a g a t a c g g a a g g a a t g t c t c c t g c t a a g g t a t a t a a c t a g c t t t t t a t g g c g a c g c a t t t t c t a t g c c t t c c t t a c a g a g g a c g a t t c c a t a t a t t

13,260 35 40 45 50 LVGENENLYLKMTDSRYKGT KanR LVGENENLYLKMTDSRYKGT g c t g g t g g g a g a a a a t g a a a a c c t a t a t t t a a a a a t g a c g g a c a g c c g g t a t a a a g g g a c c g a c c a c c c t c t t t t a c t t t t g g a t a t a a a t t t t t a c t g c c t g t c g g c c a t a t t t c c c t g

13,320 55 60 65 70 TYDVEREKDMMLWLEGKLPV KanR TYDVEREKDMMLWLEGKLPV c a c c t a t g a t g t g g a a c g g g a a a a g g a c a t g a t g c t a t g g c t g g a a g g a a a g c t g c c t g t g t g g a t a c t a c a c c t t g c c c t t t t c c t g t a c t a c g a t a c c g a c c t t c c t t t c g a c g g a c a

13,380 75 80 85 90 PKVLHFERHDGWSNLLMSEA KanR PKVLHFERHDGWSNLLMSEA t c c a a a g g t c c t g c a c t t t g a a c g g c a t g a t g g c t g g a g c a a t c t g c t c a t g a g t g a g g c a g g t t t c c a g g a c g t g a a a c t t g c c g t a c t a c c g a c c t c g t t a g a c g a g t a c t c a c t c c g

13,440 95 100 105 110 DGVLCSEEYEDEQSPEKIIE KanR DGVLCSEEYEDEQSPEKIIE c g a t g g c g t c c t t t g c t c g g a a g a g t a t g a a g a t g a a c a a a g c c c t g a a a a g a t t a t c g a g c t a c c g c a g g a a a c g a g c c t t c t c a t a c t t c t a c t t g t t t c g g g a c t t t t c t a a t a g c t

13,500 115 120 125 130 LYAECIRLFHSIDISDCPYT KanR LYAECIRLFHSIDISDCPYT g c t g t a t g c g g a g t g c a t c a g g c t c t t t c a c t c c a t c g a c a t a t c g g a t t g t c c c t a t a c c g a c a t a c g c c t c a c g t a g t c c g a g a a a g t g a g g t a g c t g t a t a g c c t a a c a g g g a t a t g

13,560 135 140 145 150 NSLDSRLAELDYLLNNDLAD KanR NSLDSRLAELDYLLNNDLAD g a a t a g c t t a g a c a g c c g c t t a g c c g a a t t g g a t t a c t t a c t g a a t a a c g a t c t g g c c g a c t t a t c g a a t c t g t c g g c g a a t c g g c t t a a c c t a a t g a a t g a c t t a t t g c t a g a c c g g c t

13,620 155 160 165 170 VDCENWEEDTPFKDPRELYD KanR VDCENWEEDTPFKDPRELYD t g t g g a t t g c g a a a a c t g g g a a g a a g a c a c t c c a t t t a a a g a t c c g c g c g a g c t g t a t g a a c a c c t a a c g c t t t t g a c c c t t c t t c t g t g a g g t a a a t t t c t a g g c g c g c t c g a c a t a c t

13,680 175 180 185 190 FLKTEKPEEELVFSHGDLGD KanR FLKTEKPEEELVFSHGDLGD t t t t t t a a a g a c g g a a a a g c c c g a a g a g g a a c t t g t c t t t t c c c a c g g c g a c c t g g g a g a a a a a a a t t t c t g c c t t t t c g g g c t t c t c c t t g a a c a g a a a a g g g t g c c g c t g g a c c c t c t

13,740 195 200 205 210 SNIFVKDGKVSGFIDLGRSG KanR SNIFVKDGKVSGFIDLGRSG c a g c a a c a t c t t t g t g a a a g a t g g c a a a g t a a g t g g c t t t a t t g a t c t t g g g a g a a g c g g g t c g t t g t a g a a a c a c t t t c t a c c g t t t c a t t c a c c g a a a t a a c t a g a a c c c t c t t c g c c

13,800 215 220 225 230 RADKWYDIAFCVRSIREDIG KanR RADKWYDIAFCVRSIREDIG c a g g g c g g a c a a g t g g t a t g a c a t t g c c t t c t g c g t c c g g t c g a t c a g g g a g g a t a t c g g g t c c c g c c t g t t c a c c a t a c t g t a a c g g a a g a c g c a g g c c a g c t a g t c c c t c c t a t a g c c

13,860 235 240 245 250 EEQYVELFFDLLGIKPDWEK KanR EEQYVELFFDLLGIKPDWEK g g a a g a a c a g t a t g t c g a g c t a t t t t t t g a c t t a c t g g g g a t c a a g c c t g a t t g g g a g a a c c t t c t t g t c a t a c a g c t c g a t a a a a a a c t g a a t g a c c c c t a g t t c g g a c t a a c c c t c t t

NsiI 13,920 255 260 265 IKYYILLDELF* KanR IKYYILLDELF* a a t a a a a t a t t a t a t t t t a c t g g a t g a a t t g t t t t a g t a c c t a g a a t g c a t g a c c a a a a t t t a t t t t a t a a t a t a a a a t g a c c t a c t t a a c a a a a t c a t g g a t c t t a c g t a c t g g t t t t a

13,980 *LKGLAYGHTFLN c c c t t a a c g c t g a g a g a t c c c c t c a t a a t t t c c c c a a a g c g t a a c c a t g t g t g a a t a a a t g g g a a t t g c g a c t c t c t a g g g g a g t a t t a a a g g g g t t t c g c a t t g g t a c a c a c t t a t t t a

14,040 QALLTAAVLLDERTITYGSH t t t g a g c t a g t a g g g t t g c a g c c a c g a g t a a g t c t t c c c t t g t t a t t g t g t a g c c a g a a t a a a c t c g a t c a t c c c a a c g t c g g t g c t c a t t c a g a a g g g a a c a a t a a c a c a t c g g t c t t a

14,100 RLVEMGLRVTSLVNRNRVTN g c c g c a a a a c t t c c a t g c c t a a g c g a a c t g t t g a g a g t a c g t t t c g a t t t c t g a c t g t g t c g g c g t t t t g a a g g t a c g g a t t c g c t t g a c a a c t c t c a t g c a a a g c t a a a g a c t g a c a c a

14,160 AQFHKDWGQKQAHVGALWCT t a g c c t g g a a g t g c t t g t c c c a a c c t t g t t t c t g a g c a t g a a c g c c c g c a a g c c a a c a t g a t c g g a c c t t c a c g a a c a g g g t t g g a a c a a a g a c t c g t a c t t g c g g g c g t t c g g t t g t a c

14,220 LQLMLAILLMIDFRESSSTR t t a g t t g a a g c a t c a g g g c g a t t a g c a g c a t g a t a t c a a a a c g c t c t g a g c t g c t c g t t c a a t c a a c t t c g t a g t c c c g c t a a t c g t c g t a c t a t a g t t t t g c g a g a c t c g a c g a g c a a g

StuI 14,280 SHRLGLGYAPSKLDRFTEEI g g c t a t g g c g t a g g c c t a g t c c g t a g g c a g g a c t t t t c a a g t c t c g g a a g g t t t c t t c a a c c g a t a c c g c a t c c g g a t c a g g c a t c c g t c c t g a a a a g t t c a g a g c c t t c c a a a g a a g t t

14,340 QMRKSYINVLQKPTRIEVPL t c t g c a t t c g c t t c g a a t a g a t a t t a a c a a g t t g t t t g g g t g t t c g a a t t t c a a c a g g t a a g a c g t a a g c g a a g c t t a t c t a t a a t t g t t c a a c a a a c c c a c a a g c t t a a a g t t g t c c a t

14,400 NTALIWPEKASASYIKPSPH a g t t a g t t g c t a g a a t c c a t g g c t c c t t t g c c g a c g c t g a g t a g a t t t t a g g t g a c g g g t t c a a t c a a c g a t c t t a g g t a c c g a g g a a a c g g c t g c g a c t c a t c t a a a a t c c a c t g c c c a

14,460 HCHTRTSRQNKRGKSRSKYL g g t g a c a a t g a g t c c g t g t c g a g c g c t g a t t t t t t c g g c c t t t a g a g c g a g a t t t a t a c a c c a c t g t t a c t c a g g c a c a g c t c g c g a c t a a a a a a g c c g g a a a t c t c g c t c t a a a t a t g t

14,520 LIQCSIPNSKTLRKYGLTKS a t a g a a t t t g g c a t g a g a t t g g a t t g c t t t t a g t c a g c c t c t t a t a g c c t a a a g t c t t t g t a t c t t a a a c c g t a c t c t a a c c t a a c g a a a a t c a g t c g g a g a a t a t c g g a t t t c a g a a a c

AvrII 14,580 HSSSMDHLNSIPKWNEAGLD a g t g a c t a g a t g a c a t a t c a t g t a a g t t g c t g a t a g g t t t c c a g t t t t c c g c t c c t a g g t t c a c t g a t c t a c t g t a t a g t a c a t t c a a c g a c t a t c c a a a g g t c a a a a g g c g a g g a t c c a

14,640 AYQVKGRVRSLWYWGLKEVS c t g c a t a t t g t a c t t t t c c t c t t a c t c g a c t t a a c c a g t a c c a a c c c a g c t t c t c a a c g g g a c g t a t a a c a t g a a a a g g a g a a t g a g c t g a a t t g g t c a t g g t t g g g t c g a a g a g t t g c c

14,700 KYWPVKFGADSVILPTTNSP a t t t a t a c c a t g g c a c t t t a a a g c c a g c a t c a c t g a c a a t g a g c g g t g t g g t g t t a c t c g t a a a t a t g g t a c c g t g a a a t t t c g g t c g t a g t g a c t g t t a c t c g c c a c a c c a c a a t g a g c

14,760 LISALDALFQDHAKKSCQES g t a g a a t g c t c g c a a g g t c g g c t a g a a a t t g g t c a t g a g c t t t c t t t g a a c a t t g c t c t g c a t c t t a c g a g c g t t c c a g c c g a t c t t t a a c c a g t a c t c g a a a g a a a c t t g t a a c g a g a c

14,820 LPFAKEYLTVSRGHLAVSAR a a a g c g g g a a c g c t t t c t c a t a a a g a g t a a c a g a a c g a c c g t g t a g t g c g a c t g a a g c t c t t t c g c c c t t g c g a a a g a g t a t t t c t c a t t g t c t t g c t g g c a c a t c a c g c t g a c t t c g a g

14,880 LVMLRKQERIDSWDVLVIPM g c a a t a c c a t a a g c c g t t t t t g c t c a c g g a t a t c a g a c c a g t c a a c a a g t a c a a t g g g c a c g t t a t g g t a t t c g g c a a a a a c g a g t g c c t a t a g t c t g g t c a g t t g t t c a t g t t a c c c g t

14,940 TNGSCIFSAHWRYVALREKH t c g t a t t g c c c g a a c a g a t a a a g c t a g c a t g c c a a c g g t a t a c a g c g a g t c g c t c t t t g t a g c a t a a c g g g c t t g t c t a t t t c g a t c g t a c g g t t g c c a t a t g t c g c t c a g c g a g a a a c a

15,000 LHRNGLLRDIRKINHKTRAK g g a g g t g a c g a t t a c c t a a c a a t c g g t c g a t t c g t t t g a t g t t a t g t t t t g t t c t c g c t t c c t c c a c t g c t a a t g g a t t g t t a g c c a g c t a a g c a a a c t a c a a t a c a a a a c a a g a g c g a a

15,060 TPLNRGLETLTLTKCDLLAH t g g t t g g c a g g t t a c g g c c a a g t t c g g t a a g a g t g a g a g t t t t a c a g t c a a g t a a g g c g t a c c a a c c g t c c a a t g c c g g t t c a a g c c a t t c t c a c t c t c a a a a t g t c a g t t c a t t c c g c a

15,120 CALTLSNLRKLHLEPCFQYL g g c a a g c c a a c g t t a a g c t g t t g a g t c g t t t t a a g t g t a a t t c g g g g c a g a a t t g g t a a a c c g t t c g g t t g c a a t t c g a c a a c t c a g c a a a a t t c a c a t t a a g c c c c g t c t t a a c c a t t t

15,180 SDHLIDLECM g a g a g t c g t g t a a a a t a t c g a g t t c g c a c a t t t t g t t g t c t g a t t a t t g a t t t t t g g c g a c t c t c a g c a c a t t t t a t a g c t c a a g c g t g t a a a a c a a c a g a c t a a t a a c t a a a a a c c g c t

15,240 a a c c a t t t g a t c a t a t g a c a a g a t g t g t a t c t a c c t t a a c t t a a t g a t t t t g a t a a a a a t t t g g t a a a c t a g t a t a c t g t t c t a c a c a t a g a t g g a a t t g a a t t a c t a a a a c t a t t t t t a

15,300 c a t t a g g g g a t t c a t c a g c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t g t a a t c c c c t a a g t a g t c g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a

3ʹ 5ʹ 15,321 a g a a a a g a t c a a a g g a t c t t c t c t t t t c t a g t t t c c t a g a a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.