Addgene iRFP713-FRB-PIP5K1A - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: iRFP713-FRB-PIP5K1A


Map View

pBR322ori-F (6764 .. 6783) ApaLI (6605) TK-pA-R (5855 .. 5874) Neo-F (5537 .. 5556) RsrII (5517) BsrDI (5234) MscI (5083) Neo-R (4927 .. 4946) BspDI * - ClaI * (4841) SfiI (4776) CsiI - SexAI * (4590) pBABE 3' (4504 .. 4524) F1ori-R (4242 .. 4261) F1ori-F (4030 .. 4051) MluI (3887) EBV-rev (3856 .. 3875) SV40pA-R (3802 .. 3821) HpaI (3764) MfeI (3751) AseI (7) SnaBI (340) CMV-F (519 .. 539) NheI (591) BmtI (595) AgeI (600) TspMI - XmaI (859) SmaI (861) Eco53kI (963) SacI (965) SbfI (1040) EcoRV (1275) BspEI (1561) BglII (1570) EcoNI (1642) PspOMI (1728) ApaI (1732) SalI (1930) AccI (1931) BbvCI - Bpu10I (2025) PaqCI (2635) BsrGI (2843) AflII (2961) ScaI (2980) XmnI (3128) iRFP713-FRB-PIP5K1A 6977 bp

Sequence View

AseI 5ʹ 3ʹ 60 t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c

120 CMV enhancer c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a

180 CMV enhancer g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t

240 CMV enhancer a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g

300 CMV enhancer a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t

SnaBI 360 CMV enhancer c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g

420 CMV enhancer CMV promoter c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c

480 CMV promoter *HSNTKAGFDV a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F PSELIDYCSRGMSAFPRYAH g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a

BmtINheI 600 CMV promoter VTPPRYLCLQNLSGDSGSAS a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t

AgeI 660 1 5 10 15 MAEGSVARQPDLLTCD iRFP713 Kozak sequence MAEGSVARQPDLLTCD GTAVMASPDTALCGSRKVQS c c g g t c g c c a c c a t g g c g g a a g g a t c c g t c g c c a g g c a g c c t g a c c t c t t g a c c t g c g a c g g c c a g c g g t g g t a c c g c c t t c c t a g g c a g c g g t c c g t c g g a c t g g a g a a c t g g a c g c t g

720 20 25 30 35 DEPIHIPGAIQPHGLLLALA iRFP713 DEPIHIPGAIQPHGLLLALA SSGIWIGPAMWGCPSSSARA g a t g a g c c g a t c c a t a t c c c c g g t g c c a t c c a a c c g c a t g g a c t g c t g c t c g c c c t c g c c c t a c t c g g c t a g g t a t a g g g g c c a c g g t a g g t t g g c g t a c c t g a c g a c g a g c g g g a g c g g

780 40 45 50 55 ADMTIVAGSDNLPELTGLAI iRFP713 ADMTIVAGSDNLPELTGLAI ASMVITAPLSLRGSSVPSAI g c c g a c a t g a c g a t c g t t g c c g g c a g c g a c a a c c t t c c c g a a c t c a c c g g a c t g g c g a t c c g g c t g t a c t g c t a g c a a c g g c c g t c g c t g t t g g a a g g g c t t g a g t g g c c t g a c c g c t a g

840 60 65 70 75 GALIGRSAADVFDSETHNRL iRFP713 GALIGRSAADVFDSETHNRL PARIPREAASTKSESVCLRR g g c g c c c t g a t c g g c c g c t c t g c g g c c g a t g t c t t c g a c t c g g a g a c g c a c a a c c g t c t g c c g c g g g a c t a g c c g g c g a g a c g c c g g c t a c a g a a g c t g a g c c t c t g c g t g t t g g c a g a c

TspMIXmaISmaI 900 80 85 90 95 TIALAEPGAAVGAPITVGFT iRFP713 TIALAEPGAAVGAPITVGFT VIAKASGPAATPAGIVTPKV a c g a t c g c c t t g g c c g a g c c c g g g g c g g c c g t c g g a g c a c c g a t c a c t g t c g g c t t c a c g t g c t a g c g g a a c c g g c t c g g g c c c c g c c g g c a g c c t c g t g g c t a g t g a c a g c c g a a g t g c

960 100 105 110 115 MRKDAGFIGSWHRHDQLIFL iRFP713 MRKDAGFIGSWHRHDQLIFL IRFSAPKM a t g c g a a a g g a c g c a g g c t t c a t c g g c t c c t g g c a t c g c c a t g a t c a g c t c a t c t t c c t c t a c g c t t t c c t g c g t c c g a a g t a g c c g a g g a c c g t a g c g g t a c t a g t c g a g t a g a a g g a g

Eco53kISacI 1020 120 125 130 135 ELEPPQRDVAEPQAFFRRTN iRFP713 ELEPPQRDVAEPQAFFRRTN g a g c t c g a g c c t c c c c a g c g g g a c g t c g c c g a g c c g c a g g c g t t c t t c c g c c g c a c c a a c c t c g a g c t c g g a g g g g t c g c c c t g c a g c g g c t c g g c g t c c g c a a g a a g g c g g c g t g g t t g

SbfI 1080 140 145 150 155 SAIRRLQAAETLESACAAAA iRFP713 SAIRRLQAAETLESACAAAA a g c g c c a t c c g c c g c c t g c a g g c c g c c g a a a c c t t g g a a a g c g c c t g c g c c g c c g c g g c g t c g c g g t a g g c g g c g g a c g t c c g g c g g c t t t g g a a c c t t t c g c g g a c g c g g c g g c g c c g c

1140 160 165 170 175 QEVRKITGFDRVMIYRFASD iRFP713 QEVRKITGFDRVMIYRFASD c a a g a g g t g c g g a a g a t t a c c g g c t t c g a t c g g g t g a t g a t c t a t c g c t t c g c c t c c g a c g t t c t c c a c g c c t t c t a a t g g c c g a a g c t a g c c c a c t a c t a g a t a g c g a a g c g g a g g c t g

1200 180 185 190 195 FSGEVIAEDRCAEVESKLGL iRFP713 FSGEVIAEDRCAEVESKLGL t t c a g c g g c g a a g t g a t c g c a g a g g a t c g g t g c g c c g a g g t c g a g t c a a a a c t a g g c c t g a a g t c g c c g c t t c a c t a g c g t c t c c t a g c c a c g c g g c t c c a g c t c a g t t t t g a t c c g g a c

1260 200 205 210 215 HYPASTVPAQARRLYTINPV iRFP713 HYPASTVPAQARRLYTINPV c a c t a t c c t g c c t c a a c c g t g c c g g c g c a g g c c c g t c g g c t c t a t a c c a t c a a c c c g g t a g t g a t a g g a c g g a g t t g g c a c g g c c g c g t c c g g g c a g c c g a g a t a t g g t a g t t g g g c c a t

EcoRV 1320 220 225 230 235 RIIPDINYRPVPVTPDLNPV iRFP713 RIIPDINYRPVPVTPDLNPV c g g a t c a t t c c c g a t a t c a a t t a t c g g c c g g t g c c g g t c a c c c c a g a c c t c a a t c c g g t c g c c t a g t a a g g g c t a t a g t t a a t a g c c g g c c a c g g c c a g t g g g g t c t g g a g t t a g g c c a g

1380 240 245 250 255 TGRPIDLSFAILRSVSPVHL iRFP713 TGRPIDLSFAILRSVSPVHL a c c g g g c g g c c g a t t g a t c t t a g c t t c g c c a t c c t g c g c a g c g t c t c g c c c g t c c a t c t g t g g c c c g c c g g c t a a c t a g a a t c g a a g c g g t a g g a c g c g t c g c a g a g c g g g c a g g t a g a c

1440 260 265 270 275 EFMRNIGMHGTMSISILRGE iRFP713 EFMRNIGMHGTMSISILRGE g a a t t c a t g c g c a a c a t a g g c a t g c a c g g c a c g a t g t c g a t c t c g a t t t t g c g c g g c g a g c t t a a g t a c g c g t t g t a t c c g t a c g t g c c g t g c t a c a g c t a g a g c t a a a a c g c g c c g c t c

1500 280 285 290 295 RLWGLIVCHHRTPYYVDLDG iRFP713 RLWGLIVCHHRTPYYVDLDG c g a c t g t g g g g a t t g a t c g t t t g c c a t c a c c g a a c g c c g t a c t a c g t c g a t c t c g a t g g c g c t g a c a c c c c t a a c t a g c a a a c g g t a g t g g c t t g c g g c a t g a t g c a g c t a g a g c t a c c g

1560 300 305 310 315 RQACELVAQVLAWQIGVMEE iRFP713 RQACELVAQVLAWQIGVMEE c g c c a a g c c t g c g a g c t a g t c g c c c a g g t t c t g g c c t g g c a g a t c g g c g t g a t g g a a g a g g c g g t t c g g a c g c t c g a t c a g c g g g t c c a a g a c c g g a c c g t c t a g c c g c a c t a c c t t c t c

BglIIBspEI 1620 (in frame with iRFP713) SGLRSRAGGAGAILSR ILWH FRB SGLRSRAGGAGAILSRILWH t c c g g a c t c a g a t c t c g a g c t g g a g g t g c t g g t g c t a t c c t a t c t a g a a t c c t c t g g c a t a g g c c t g a g t c t a g a g c t c g a c c t c c a c g a c c a c g a t a g g a t a g a t c t t a g g a g a c c g t a

EcoNI 1680 2025 2030 2035 2040 EMWHEGLEEASRLYFGERNV FRB EMWHEGLEEASRLYFGERNV g a g a t g t g g c a t g a a g g c c t g g a a g a g g c a t c t c g t t t g t a c t t t g g g g a a a g g a a c g t g c t c t a c a c c g t a c t t c c g g a c c t t c t c c g t a g a g c a a a c a t g a a a c c c c t t t c c t t g c a c

PspOMIApaI 1740 2045 2050 2055 2060 KGMFEVLEPLHAMMERGPQT FRB KGMFEVLEPLHAMMERGPQT a a a g g c a t g t t t g a g g t g c t g g a g c c c t t g c a t g c t a t g a t g g a a c g g g g c c c c c a g a c t t t t c c g t a c a a a c t c c a c g a c c t c g g g a a c g t a c g a t a c t a c c t t g c c c c g g g g g t c t g a

1800 2065 2070 2075 2080 LKETSFNQAYGRDLMEAQEW FRB LKETSFNQAYGRDLMEAQEW c t g a a g g a a a c a t c c t t t a a t c a g g c c t a t g g t c g a g a t t t a a t g g a g g c c c a a g a g t g g g a c t t c c t t t g t a g g a a a t t a g t c c g g a t a c c a g c t c t a a a t t a c c t c c g g g t t c t c a c c

1860 2085 2090 2095 2100 CRKYMKSGNVKDLTQAWDLY FRB CRKYMKSGNVKDLTQAWDLY t g c a g g a a g t a c a t g a a a t c a g g g a a t g t c a a g g a c c t c a c c c a a g c c t g g g a c c t c t a t a c g t c c t t c a t g t a c t t t a g t c c c t t a c a g t t c c t g g a g t g g g t t c g g a c c c t g g a g a t a

1920 2105 2110 YHVFRRISK (in frame with FRB) GGSAGGSAQAS FRB YHVFRRISKGGSAGGSAQAS t a t c a t g t g t t c c g a c g a a t c t c a a a g g g t g g t a g t g c t g g t g g t a g t g c t c a a g c t t c g a t a g t a c a c a a g g c t g c t t a g a g t t t c c c a c c a t c a c g a c c a c c a t c a c g a g t t c g a a g c

SalIAccI 1980 (in frame with FRB) NSAVDGTAMASASSGPSSSV NSAVDGTAMASASSGPSSSV a a t t c t g c a g t c g a c g g t a c c g c g a t g g c g t c g g c c t c c t c c g g g c c g t c g t c t t c g g t c t t a a g a c g t c a g c t g c c a t g g c g c t a c c g c a g c c g g a g g a g g c c c g g c a g c a g a a g c c a g

Bpu10IBbvCI 2040 (in frame with FRB) GFSSFDPAVPSCTLSSAASG GFSSFDPAVPSCTLSSAASG g g t t t t t c a t c c t t t g a t c c c g c g g t c c c t t c c t g t a c c t t g t c c t c a g c a g c a t c t g g a c c a a a a a g t a g g a a a c t a g g g c g c c a g g g a a g g a c a t g g a a c a g g a g t c g t c g t a g a c c t

2100 (in frame with FRB) IKRPMASEVLEARQDSYISL IKRPMASEVLEARQDSYISL a t c a a g a g a c c c a t g g c a t c t g a g g t c t t g g a a g c t a g a c a g g a t t c t t a c a t c t c a t t g t a g t t c t c t g g g t a c c g t a g a c t c c a g a a c c t t c g a t c t g t c c t a a g a a t g t a g a g t a a c

2160 (in frame with FRB) VPYASGMPIKKIGHRSVDSS VPYASGMPIKKIGHRSVDSS g t g c c t t a t g c c t c t g g c a t g c c c a t c a a g a a a a t a g g c c a t a g a a g t g t t g a t t c c t c a c a c g g a a t a c g g a g a c c g t a c g g g t a g t t c t t t t a t c c g g t a t c t t c a c a a c t a a g g a g t

2220 (in frame with FRB) GETTYKKTTSSALKGAIQLG GETTYKKTTSSALKGAIQLG g g a g a g a c a a c a t a t a a a a a g a c a a c c t c a t c a g c c t t g a a a g g t g c c a t c c a g t t a g g c c c t c t c t g t t g t a t a t t t t t c t g t t g g a g t a g t c g g a a c t t t c c a c g g t a g g t c a a t c c g

2280 (in frame with FRB) ITHTVGSLSTKPERDVLMQD ITHTVGSLSTKPERDVLMQD a t t a c c c a c a c t g t g g g g a g c c t g a g t a c c a a a c c a g a g c g t g a t g t c c t c a t g c a a g a t t a a t g g g t g t g a c a c c c c t c g g a c t c a t g g t t t g g t c t c g c a c t a c a g g a g t a c g t t c t a

2340 (in frame with FRB) FYVVESIFFPSEGSNLTPAH FYVVESIFFPSEGSNLTPAH t t c t a c g t g g t t g a g a g t a t c t t c t t t c c c a g t g a a g g g a g c a a c c t g a c c c c t g c t c a t a a g a t g c a c c a a c t c t c a t a g a a g a a a g g g t c a c t t c c c t c g t t g g a c t g g g g a c g a g t a

2400 (in frame with FRB) HYNDFRFKTYAPVAFRYFRE HYNDFRFKTYAPVAFRYFRE c a c t a c a a t g a c t t t c g t t t c a a g a c c t a t g c a c c t g t t g c c t t c c g c t a c t t c c g g g a g g t g a t g t t a c t g a a a g c a a a g t t c t g g a t a c g t g g a c a a c g g a a g g c g a t g a a g g c c c t c

2460 (in frame with FRB) LFGIRPDDYLYSLCSEPLIE LFGIRPDDYLYSLCSEPLIE c t a t t t g g t a t c c g g c c c g a t g a t t a c t t g t a t t c c c t c t g c a g t g a g c c g c t g a t t g a a g a t a a a c c a t a g g c c g g g c t a c t a a t g a a c a t a a g g g a g a c g t c a c t c g g c g a c t a a c t t

2520 (in frame with FRB) LCSSGASGSLFYVSSDDEFI LCSSGASGSLFYVSSDDEFI c t c t g t a g c t c t g g a g c t a g t g g t t c c c t a t t c t a t g t g t c c a g c g a c g a t g a g t t c a t t g a g a c a t c g a g a c c t c g a t c a c c a a g g g a t a a g a t a c a c a g g t c g c t g c t a c t c a a g t a a

2580 (in frame with FRB) IKTVQHKEAEFLQKLLPGYY IKTVQHKEAEFLQKLLPGYY a t t a a g a c a g t c c a a c a t a a a g a g g c g g a a t t t c t g c a g a a g c t g c t t c c a g g a t a c t a c t a a t t c t g t c a g g t t g t a t t t c t c c g c c t t a a a g a c g t c t t c g a c g a a g g t c c t a t g a t g

PaqCI 2640 (in frame with FRB) MNLNQNPRTLLPKFYGLYCV MNLNQNPRTLLPKFYGLYCV a t g a a c c t c a a c c a g a a c c c t c g g a c t t t g c t g c c t a a a t t c t a t g g a c t g t a c t g t g t g t a c t t g g a g t t g g t c t t g g g a g c c t g a a a c g a c g g a t t t a a g a t a c c t g a c a t g a c a c a c

2700 (in frame with FRB) QAGGKNIRIVVMNNLLPRSV QAGGKNIRIVVMNNLLPRSV c a g g c a g g t g g c a a g a a c a t t c g g a t t g t g g t g a t g a a c a a t c t t t t a c c a a g a t c g g t a g t c c g t c c a c c g t t c t t g t a a g c c t a a c a c c a c t a c t t g t t a g a a a a t g g t t c t a g c c a t

2760 (in frame with FRB) KMHIKYDLKGSTYKRRASQK KMHIKYDLKGSTYKRRASQK a a a a t g c a t a t c a a a t a t g a c c t c a a a g g c t c a a c c t a c a a a c g g c g g g c t t c c c a g a a a t t t t a c g t a t a g t t t a t a c t g g a g t t t c c g a g t t g g a t g t t t g c c g c c c g a a g g g t c t t t

2820 (in frame with FRB) EREKPLPTFKDLDFLQDIPD EREKPLPTFKDLDFLQDIPD g a g c g a g a g a a g c c t c t t c c c a c a t t t a a a g a c c t a g a c t t c t t a c a a g a c a t c c c t g a t c t c g c t c t c t t c g g a g a a g g g t g t a a a t t t c t g g a t c t g a a g a a t g t t c t g t a g g g a c t a

BsrGI 2880 (in frame with FRB) GLFLDADMYNALCKTLQRDC GLFLDADMYNALCKTLQRDC g g t c t t t t t t t g g a t g c t g a c a t g t a c a a c g c t c t c t g t a a g a c c c t g c a g c g t g a c t g t c c a g a a a a a a a c c t a c g a c t g t a c a t g t t g c g a g a g a c a t t c t g g g a c g t c g c a c t g a c a

2940 (in frame with FRB) LVLQSFKIMDYSLLMSIHNI LVLQSFKIMDYSLLMSIHNI t t g g t g c t g c a g a g c t t c a a g a t a a t g g a t t a c a g c c t c t t g a t g t c a a t c c a t a a t a t a a a c c a c g a c g t c t c g a a g t t c t a t t a c c t a a t g t c g g a g a a c t a c a g t t a g g t a t t a t a t

ScaIAflII 3000 (in frame with FRB) DHAQREPLSSETQYSVDTRR DHAQREPLSSETQYSVDTRR g a t c a t g c a c a a c g a g a g c c c t t a a g c a g t g a a a c a c a g t a c t c a g t t g a t a c t c g a a g a c t a g t a c g t g t t g c t c t c g g g a a t t c g t c a c t t t g t g t c a t g a g t c a a c t a t g a g c t t c t

3060 (in frame with FRB) PAPQKALYSTAMESIQGEAR PAPQKALYSTAMESIQGEAR c c g g c c c c c c a a a a g g c t c t g t a t t c c a c a g c c a t g g a a t c c a t c c a g g g a g a g g c t c g a g g c c g g g g g g t t t t c c g a g a c a t a a g g t g t c g g t a c c t t a g g t a g g t c c c t c t c c g a g c t

3120 (in frame with FRB) RGGTMETDDHMGGIPARNSK RGGTMETDDHMGGIPARNSK c g g g g t g g t a c c a t g g a g a c t g a t g a c c a t a t g g g t g g c a t c c c t g c c c g g a a t a g t a a a g c c c c a c c a t g g t a c c t c t g a c t a c t g g t a t a c c c a c c g t a g g g a c g g g c c t t a t c a t t t

XmnI 3180 (in frame with FRB) GERLLLYIGIIDILQSYRFV GERLLLYIGIIDILQSYRFV g g g g a a a g g c t t c t g c t t t a t a t t g g c a t c a t t g a c a t t c t a c a g t c t t a c a g g t t t g t t c c c c t t t c c g a a g a c g a a a t a t a a c c g t a g t a a c t g t a a g a t g t c a g a a t g t c c a a a c a a

3240 (in frame with FRB) KKLEHSWKALVHDGDTVSVH KKLEHSWKALVHDGDTVSVH a a g a a g t t g g a g c a c t c t t g g a a a g c c c t g g t a c a t g a c g g a g a c a c t g t c t c a g t g c a t t t c t t c a a c c t c g t g a g a a c c t t t c g g g a c c a t g t a c t g c c t c t g t g a c a g a g t c a c g t a

3300 (in frame with FRB) RPGFYAERFQRFMCNTVFKK RPGFYAERFQRFMCNTVFKK c g c c c a g g c t t c t a c g c t g a a c g g t t c c a g c g c t t c a t g t g c a a c a c a g t a t t t a a g a a g g c g g g t c c g a a g a t g c g a c t t g c c a a g g t c g c g a a g t a c a c g t t g t g t c a t a a a t t c t t c

3360 (in frame with FRB) IPLKPSPSKKFRSGSSFSRR IPLKPSPSKKFRSGSSFSRR a t t c c c t t g a a g c c t t c t c c t t c c a a a a a g t t t c g g t c t g g c t c a t c t t t c t c t c g g c g a t a a g g g a a c t t c g g a a g a g g a a g g t t t t t c a a a g c c a g a c c g a g t a g a a a g a g a g c c g c t

3420 (in frame with FRB) AGSSGNSCITYQPSVSGEHK AGSSGNSCITYQPSVSGEHK g c a g g c t c c a g t g g c a a c t c c t g c a t t a c t t a c c a g c c a t c g g t c t c t g g g g a a c a c a a g c g t c c g a g g t c a c c g t t g a g g a c g t a a t g a a t g g t c g g t a g c c a g a g a c c c c t t g t g t t c

3480 (in frame with FRB) AQVTTKAEVEPGVHLGRPDV AQVTTKAEVEPGVHLGRPDV g c a c a a g t g a c a a c a a a g g c a g a a g t g g a g c c a g g c g t t c a c c t t g g t c g t c c t g a t g t t c g t g t t c a c t g t t g t t t c c g t c t t c a c c t c g g t c c g c a a g t g g a a c c a g c a g g a c t a c a a

3540 (in frame with FRB) LPQTPPLEEISEGSPIPDPS LPQTPPLEEISEGSPIPDPS t t a c c t c a g a c t c c a c c t t t g g a g g a a a t c a g t g a g g g c t c g c c t a t t c c t g a c c c c a g t a a t g g a g t c t g a g g t g g a a a c c t c c t t t a g t c a c t c c c g a g c g g a t a a g g a c t g g g g t c a

3600 (in frame with FRB) FSPLVGETLQMLTTSTTLEK FSPLVGETLQMLTTSTTLEK t t c t c a c c t c t a g t t g g a g a g a c t t t g c a a a t g c t a a c t a c a a g t a c a a c c t t g g a a a a g a a g a g t g g a g a t c a a c c t c t c t g a a a c g t t t a c g a t t g a t g t t c a t g t t g g a a c c t t t t c

3660 (in frame with FRB) LEVAESEFTH* LEVAESEFTH* c t t g a a g t t g c a g a g t c a g a g t t c a c c c a t t a a c g g g a t c c a c c g g a t c t a g a t a a c t g a g a a c t t c a a c g t c t c a g t c t c a a g t g g g t a a t t g c c c t a g g t g g c c t a g a t c t a t t g a c t

3720 t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g

HpaIMfeI 3780 SV40 poly(A) signal t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c

3840 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a

MluI 3900 SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a c g c g t a a a t t g t a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t g c g c a t t t a a c a t

3960 f1 ori a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g

4020 f1 ori c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c

4080 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t

4140 f1 ori g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a

4200 f1 ori t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a

4260 f1 ori A G G G A A G A A A G C G A A A G G A F1ori-R a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t

4320 f1 ori G F1ori-R g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g

4380 f1 ori AmpR promoter g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t

4440 AmpR promoter a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t

4500 AmpR promoter SV40 promoter SV40 enhancer c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g

4560 SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t

SexAI*CsiI 4620 SV40 promoter SV40 enhancer t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c

4680 SV40 promoter SV40 enhancer c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t

4740 SV40 promoter SV40 ori c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g

SfiI 4800 SV40 promoter SV40 ori t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a

BspDI*ClaI* 4860 SV40 promoter SV40 ori a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t

4920 1 5 10 15 MIEQDGLHAGSPAAWV NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWV NeoR/KanR MIEQDGLHAGSPAAWV g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c

4980 20 25 30 35 ERLFGYDWAQQTIGCSDAAV NeoR/KanR 20 25 30 35 ERLFGYDWAQQTIGCSDAAV NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R ERLFGYDWAQQTIGCSDAAV g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c

5040 40 45 50 55 FRLSAQGRPVLFVKTDLSGA NeoR/KanR 40 45 50 55 FRLSAQGRPVLFVKTDLSGA NeoR/KanR FRLSAQGRPVLFVKTDLSGA t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g

MscI 5100 60 65 70 75 LNELQDEAARLSWLATTGVP NeoR/KanR 60 65 70 75 LNELQDEAARLSWLATTGVP NeoR/KanR LNELQDEAARLSWLATTGVP c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a

5160 80 85 90 95 CAAVLDVVTEAGRDWLLLGE NeoR/KanR 80 85 90 95 CAAVLDVVTEAGRDWLLLGE NeoR/KanR CAAVLDVVTEAGRDWLLLGE t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t

5220 100 105 110 115 VPGQDLLSSHLAPAEKVSIM NeoR/KanR 100 105 110 115 VPGQDLLSSHLAPAEKVSIM NeoR/KanR VPGQDLLSSHLAPAEKVSIM *RVKSRGLFYGDH g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c

BsrDI 5280 120 125 130 135 ADAMRRLHTLDPATCPFDHQ NeoR/KanR 120 125 130 135 ADAMRRLHTLDPATCPFDHQ NeoR/KanR ADAMRRLHTLDPATCPFDHQ SICHPPQMRKIRSGAWEVVL g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t

5340 140 145 150 155 AKHRIERARTRMEAGLVDQD NeoR/KanR 140 145 150 155 AKHRIERARTRMEAGLVDQD NeoR/KanR AKHRIERARTRMEAGLVDQD RFMADLSCTSPHFGTKDILI g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a

5400 160 165 170 175 DLDEEHQGLAPAELFARLKA NeoR/KanR 160 165 170 175 DLDEEHQGLAPAELFARLKA NeoR/KanR DLDEEHQGLAPAELFARLKA IQVFLMLPERWGFQEGPELR g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c

5460 180 185 190 195 SMPDGEDLVVTHGDACLPNI NeoR/KanR 180 185 190 195 SMPDGEDLVVTHGDACLPNI NeoR/KanR SMPDGEDLVVTHGDACLPNI AHGVALIEDHGMAIGAQRID a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g

RsrII 5520 200 205 210 215 MVENGRFSGFIDCGRLGVAD NeoR/KanR 200 205 210 215 MVENGRFSGFIDCGRLGVAD NeoR/KanR MVENGRFSGFIDCGRLGVAD HHFIAAKRSEDVTAPQTHRV a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g

5580 220 225 230 235 RYQDIALATRDIAEELGGEW NeoR/KanR 220 225 230 235 RYQDIALATRDIAEELGGEW NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F RYQDIALATRDIAEELGGEW AILVYRQSGTINSFLKAAFP c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c

5640 240 245 250 255 ADRFLVLYGIAAPDSQRIAF NeoR/KanR 240 245 250 255 ADRFLVLYGIAAPDSQRIAF NeoR/KanR ADRFLVLYGIAAPDSQRIAF SVAEEHKVTDGSGIRLADGE g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g

5700 260 265 YRLLDEFF* NeoR/KanR 260 265 YRLLDEFF* NeoR/KanR YRLLDEFF* MTDQ IAKKVLEESRSEPTRFSRGL t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c

5760 ATPNLPSRDFDSTAAFYERL SAWGAM c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c

5820 GFGIVFRDAGWMILQRGDLM g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g

5880 A C T T T G T G C C T T C C T C T G T T TK-pA-R LEFFAHPRGRLTETRKETIP t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c

5940 HSV TK poly(A) signal EGTRAMTAIKRQNKTHGVGS a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a

6000 HSV TK poly(A) signal FVHKRGVRSQGWHSVDTPPR t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g

6060 PHWGQYARVSSFSPPHPPSS c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c

6120 GEGPGLAANVGAAGPAIASG g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a

6180 YSYIL* a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t

6240 a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c

6300 ori c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t

6360 ori t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c

6420 ori a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c

6480 ori t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a

6540 ori a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t

6600 ori c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c

ApaLI 6660 ori g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g

6720 ori g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t

6780 ori G G G A A A C G C C T G G T A T C pBR322ori-F g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g

6840 ori T T T pBR322ori-F t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a

6900 ori c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a

6960 t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g

3ʹ 5ʹ 6977 g t a t t a c c g c c a t g c a t c a t a a t g g c g g t a c g t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.