Addgene TFORF0922 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: TFORF0922 Sequencing Result


Map View

T3 (9594 .. 9614) M13 rev M13 Reverse (9559 .. 9575) lac operator M13/pUC Reverse (9540 .. 9562) CAP binding site L4440 (9327 .. 9344) pBR322ori-F (9074 .. 9093) ScaI (7860) Amp-R (7772 .. 7791) F1ori-F (7265 .. 7286) F1ori-R (7055 .. 7074) M13/pUC Forward (6819 .. 6841) M13 Forward (6810 .. 6827) M13 fwd T7 (6781 .. 6800) T7 promoter EBV-rev (6556 .. 6575) SV40pA-R (6502 .. 6521) SnaBI (6391) PflMI (5735) WPRE-R (5547 .. 5567) MluI (5479) SpeI (5393) FspAI (5164) PmlI (4930) T3 promoter 5' LTR (truncated) HIV-1 Ψ NruI * (611) NotI (922) AleI (1351) NsiI (1718) Acc65I (2001) KpnI (2005) Puro-R (2020 .. 2039) BsiWI (2076) BspEI * (2133) RsrII (2136) BstEII (2154) MscI * (2395) Puro-F (2516 .. 2536) HpaI (2620) EcoRI (2624) cPPT/CTS PspXI (2825) AgeI (2998) FseI (3624) EF1a-F (4046 .. 4066) NheI (4100) BmtI (4104) BstXI (4120) MreI - SgrAI (4223) AscI (4254) TspMI - XmaI (4276) SmaI (4278) TFORF0922 9637 bp

Sequence View

5ʹ 3ʹ 60 RSV promoter t t a a t g t a g t c t t a t g c a a t a c t c t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g c a a a a t t a c a t c a g a a t a c g t t a t g a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c g t t

120 RSV promoter c a t g c c t t a c a a g g a g a g a a a a a g c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g t a c g g a a t g t t c c t c t c t t t t t c g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c a t g

180 RSV promoter g a t c g t g c c t t a t t a g g a a g g c a a c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t g a a c t a g c a c g g a a t a a t c c t t c c g t t g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a c t t

240 RSV promoter 5' LTR (truncated) t t g c c g c a t t g c a g a g a t a t t g t a t t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t c t c t c a a c g g c g t a a c g t c t c t a t a a c a t a a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a g a g a g

300 5' LTR (truncated) t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c

360 5' LTR (truncated) c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a

420 5' LTR (truncated) g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g

480 HIV-1 Ψ c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g

540 HIV-1 Ψ t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a

600 HIV-1 Ψ t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t

NruI* 660 g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a

720 t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a

780 t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c

840 g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t

900 g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t

NotI 960 MR g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c

1020 DNWRSELYKYKVVKIEPLGV g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t

1080 RRE APTKAKRRVVQREKRAVGIG g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t

1140 RRE ALFLGFLGAAGSTMGAASMT g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c

1200 RRE LTVQARQLLSGIVQQQNNLL c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c

1260 RRE RAIEAQQHLLQLTVWGIKQL a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g

1320 RRE QARILAVERYLKDQQLLGIW c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c

AleI 1380 GCSGKLICTTAVPWNASWSN g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a

1440 KSLEQIWNHTTWMEWDREIN a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g

1500 1 KN gp41 peptide NYTSLIHSLIEESQNQQEKN a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a

1560 5 10 15 EQELLELDKWASL (in frame with gp41 peptide) WNWFNIT gp41 peptide EQELLELDKWASLWNWFNIT g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t

1620 (in frame with gp41 peptide) NWLWYIKLFIMIVGGLVGLR NWLWYIKLFIMIVGGLVGLR a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t

1680 (in frame with gp41 peptide) IVFAVLSIVNRVRQGYSPLS IVFAVLSIVNRVRQGYSPLS a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c

NsiI 1740 (in frame with gp41 peptide) FQTHLPTPRGPMHCISISQQ FQTHLPTPRGPMHCISISQQ t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c a t g c a t t g c a t c t c a a t t a g t c a g c a a a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g t a c g t a a c g t a g a g t t a a t c a g t c g t t

1800 (in frame with gp41 peptide) PGVESPQAPQQAEVCKACVS SV40 promoter SV40 promoter PGVESPQAPQQAEVCKACVS c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c g t c t c a g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g c a g a g t

1860 (in frame with gp41 peptide) ISQQP* SV40 ori SV40 promoter SV40 promoter ISQQP* a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t

1920 SV40 promoter SV40 promoter SV40 ori g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c

1980 SV40 promoter SV40 promoter SV40 ori c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a

Acc65IKpnI 2040 1 5 MTEYKPT SV40 promoter PuroR T A C T G G C T C A T G T T C G G G T G Puro-R MTEYKPT t t t g c a a a a a g c t t t c t a g a g g t a c c a c c g g a g c t t a c c a t g a c c g a g t a c a a g c c c a c g a a a c g t t t t t c g a a a g a t c t c c a t g g t g g c c t c g a a t g g t a c t g g c t c a t g t t c g g g t g c

BsiWI 2100 10 15 20 25 VRLATRDDVPRAVRTLAAAF PuroR VRLATRDDVPRAVRTLAAAF g t g c g c c t c g c c a c c c g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c c a c g c g g a g c g g t g g g c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g

BstEIIBspEI*RsrII 2160 30 35 40 45 ADYPATRHTVDPDRHIERVT PuroR ADYPATRHTVDPDRHIERVT g c c g a c t a c c c c g c c a c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c c g g c t g a t g g g g c g g t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g

2220 50 55 60 65 ELQELFLTRVGLDIGKVWVA PuroR ELQELFLTRVGLDIGKVWVA g a g c t g c a a g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g c t c g a c g t t c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c

2280 70 75 80 85 DDGAAVAVWTTPESVEAGAV PuroR DDGAAVAVWTTPESVEAGAV g a c g a c g g c g c c g c c g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g c t g c t g c c g c g g c g g c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c

2340 90 95 100 105 FAEIGPRMAELSGSRLAAQQ PuroR FAEIGPRMAELSGSRLAAQQ t t c g c c g a g a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a a a g c g g c t c t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t

MscI* 2400 110 115 120 125 QMEGLLAPHRPKEPAWFLAT PuroR QMEGLLAPHRPKEPAWFLAT c a g a t g g a a g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c t a c c t t c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g

2460 130 135 140 145 VGVSPDHQGKGLGSAVVLPG PuroR VGVSPDHQGKGLGSAVVLPG g t c g g c g t c t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a c a g c c g c a g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t

2520 150 155 160 165 VEAAERAGVPAFLETSAPRN PuroR G C A A C Puro-F VEAAERAGVPAFLETSAPRN g t g g a g g c g g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c a c c t c c g c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g

2580 170 175 180 185 LPFYERLGFTVTADVEVPEG PuroR C T C C C C T T C T A C G A G C Puro-F LPFYERLGFTVTADVEVPEG c t c c c c t t c t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a g a g g g g a a g a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t

EcoRIHpaI 2640 190 195 200 PRTWCMTRKPGAS* PuroR PRTWCMTRKPGAS* c c g c g c a c c t g g t g c a t g a c c c g c a a g c c c g g t g c c a g t t a a c g a a t t c t a g a t c t t g a g g g c g c g t g g a c c a c g t a c t g g g c g t t c g g g c c a c g g t c a a t t g c t t a a g a t c t a g a a c t c

2700 cPPT/CTS a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g

2760 cPPT/CTS a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t

2820 cPPT/CTS a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a c t t t g g c g t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t g a a a c c g c

PspXI 2880 c c g g c t c g a g g c c t g c a g g t g c a a a g a t g g a t a a a g t t t t a a a c a g a g a g g a a t c t t t g c g g c c g a g c t c c g g a c g t c c a c g t t t c t a c c t a t t t c a a a a t t t g t c t c t c c t t a g a a a c g

2940 EF-1 α promoter a g c t a a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t g g c t c c g g t g c c c g t c a g t g g t c g a t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a c c g a g g c c a c g g g c a g t c a c c

AgeI 3000 EF-1 α promoter g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t t g a a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a a c t t g g

3060 EF-1 α promoter g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g c t c c g c c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c g a g g c g

3120 EF-1 α promoter c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g t t c t t g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g c a a g a a

3180 EF-1 α promoter EF-1 α intron A t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g c g g g c c t a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c g c c c g g a

3240 EF-1 α promoter EF-1 α intron A *KWRAATR g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c t g g c t g c a g t a c c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g a c c g a c g t c a t g

3300 EF-1 α promoter EF-1 α intron A SEQDRAEPQFHTPSNSAKRK g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a g g c c t t g c g c t c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c t c c g g a a c g c g a

3360 EF-1 α promoter EF-1 α intron A LLGKAEHKLQPRAQASPGGR t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t g g g g c c g c c g c a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g a c c c c g g c g g c g

3420 EF-1 α promoter EF-1 α intron A AFRTAGERRDRQKRYTELWK g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t a g c c a t t c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a t c g g t a a

3480 EF-1 α promoter EF-1 α intron A FNKIVQQSAKKRALYDQLHP t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t a a a t g c g a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a t t t a c g c

3540 EF-1 α promoter EF-1 α intron A GLDACQYKPKQPRPRRRPGH g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g g g c c c g t c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c c c g g g c a

3600 EF-1 α promoter EF-1 α intron A TGACMNPSAPGALAAVSFRV g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g a a t c g g a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c t t a g c c t

FseI 3660 EF-1 α promoter EF-1 α intron A PTTELQGAQEPAQGRAATYR c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c g t g t a t c g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g c a c a t a g

3720 EF-1 α promoter EF-1 α intron A GARPPLAPGTPVLQTLPFIA g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a a a g a t g g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t t t c t a c c

3780 EF-1 α promoter EF-1 α intron A AERGQQLSSLISSAASPLAP c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c t c g g g a g a g c g g g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g a g c c c t c t c g c c

3840 EF-1 α promoter EF-1 α intron A PHTVWVFSFPRETRLRRKM g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c g c t t c a t g t c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g c a g c g a a g t a c a

3900 EF-1 α promoter EF-1 α intron A g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g a g c t t t t g g a g t c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c t c g a a a a c c t c a

3960 EF-1 α promoter EF-1 α intron A *TPPLPKIRHLKGVSLP a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c t g a g t g g t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g a c t c a c c

4020 EF-1 α promoter EF-1 α intron A HLSFNPWSPVQHLEGQFKGK g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t t g c c c t t c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a a c g g g a a

4080 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F KLKSRPENELRLCHNLTKKK t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t t t t t c t t a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a a a a a g a a

BstXIBmtINheI 4140 EF-1 α promoter Kozak sequence EF-1 α intron A MAMVVSSWR WKLHRSALVAVMAITTLLQR c c a t t t c a g g t g t c g t g a g g c t a g c a c c g c c a c c a t g g c a a t g g t a g t t a g c a g c t g g c g g g t a a a g t c c a c a g c a c t c c g a t c g t g g c g g t g g t a c c g t t a c c a t c a a t c g t c g a c c g c

4200 DPQDDVAGGNPGGPNPAAQA SGCSSTAPPLGPPGLGAACA a g a t c c g c a g g a c g a c g t g g c c g g g g g c a a c c c c g g c g g c c c c a a c c c c g c a g c g c a g g c t c t a g g c g t c c t g c t g c a c c g g c c c c c g t t g g g g c c g c c g g g g t t g g g g c g t c g c g t c c g

AscISgrAIMreI 4260 ARGGGGGAGEQQQQAGSGAP ARPPPPPAPSCCCCAPEPAG g g c c c g c g g c g g c g g c g g c g g c g c c g g c g a g c a g c a g c a g c a g g c g g g c t c g g g c g c g c c c c g g g c g c c g c c g c c g c c g c c g c g g c c g c t c g t c g t c g t c g t c c g c c c g a g c c c g c g c g g

TspMIXmaISmaI 4320 HTPQTPGQPGAPATPGTAGD CVGCVGPWGPAGAVGPVAPS g c a c a c g c c g c a g a c c c c g g g c c a g c c c g g a g c g c c c g c c a c c c c c g g c a c g g c g g g g g a c g t g t g c g g c g t c t g g g g c c c g g t c g g g c c t c g c g g g c g g t g g g g g c c g t g c c g c c c c c t

4380 KGQGPPGSGQSQQHIECVVC LPWPGGPEPWLWCCM c a a g g g c c a g g g c c c g c c c g g t t c g g g c c a g a g c c a g c a g c a c a t c g a g t g c g t g g t g t g g t t c c c g g t c c c g g g c g g g c c a a g c c c g g t c t c g g t c g t c g t g t a g c t c a c g c a c c a c a c

4440 GDKSSGKHYGQFTCEGCKSF c g g g g a c a a g t c g a g c g g c a a g c a c t a c g g c c a a t t c a c c t g c g a g g g c t g c a a a a g t t t g c c c c t g t t c a g c t c g c c g t t c g t g a t g c c g g t t a a g t g g a c g c t c c c g a c g t t t t c a a a

4500 MPCQQELS FKRSVRRNLTYTCRANRNCP c t t c a a g a g g a g c g t c c g c a g g a a c t t a a c t t a c a c a t g c c g t g c c a a c a g g a a c t g t c c g a a g t t c t c c t c g c a g g c g t c c t t g a a t t g a a t g t g t a c g g c a c g g t t g t c c t t g a c a g g

4560 HRPAPPQPVPILPPQEVPQS IDQHHRNQCQYCRLKKCLKV c a t c g a c c a g c a c c a c c g c a a c c a g t g c c a a t a c t g c c g c c t c a a g a a g t g c c t c a a a g t g t a g c t g g t c g t g g t g g c g t t g g t c a c g g t t a t g a c g g c g g a g t t c t t c a c g g a g t t t c a

4620 GHEAGSGSARKNASNPAQSR GMRREAVQRGRMPPTQPNPG g g g c a t g a g g c g g g a a g c g g t t c a g c g a g g a a g a a t g c c t c c a a c c c a g c c c a a t c c a g g c c c g t a c t c c g c c c t t c g c c a a g t c g c t c c t t c t t a c g g a g g t t g g g t c g g g t t a g g t c c

4680 PVRTHQRGPPQRPLLPVRLH QYALTNGDPLNGHCYLSGYI c c a g t a c g c a c t c a c c a a c g g g g a c c c c c t c a a c g g c c a c t g c t a c c t g t c c g g c t a c a t g g t c a t g c g t g a g t g g t t g c c c c t g g g g g a g t t g c c g g t g a c g a t g g a c a g g c c g a t g t a

4740 LAAAARRALPHVALRQPVHA SLLLRAEPYPTSRYGSQCMQ *PLWHMC c t c g c t g c t g c t g c g c g c c g a g c c c t a c c c c a c g t c g c g c t a c g g c a g c c a g t g c a t g c a g a g c g a c g a c g a c g c g c g g c t c g g g a t g g g g t g c a g c g c g a t g c c g t c g g t c a c g t a c g t

4800 AQQHYGHREHLRAGRAPALQ PNNIMGIENICELAARLLFS GLLMIPMSFMQSSAARRSKL g c c c a a c a a c a t t a t g g g c a t c g a g a a c a t c t g c g a g c t g g c c g c g c g c c t g c t c t t c a g c g g g t t g t t g t a a t a c c c g t a g c t c t t g t a g a c g c t c g a c c g g c g c g c g g a c g a g a a g t c

4860 RRRVGPQHPLLPGSADHRPG AVEWARNIPFFPDLQITDQV ATSHARLMGKKGSRCIVSWT c g c c g t c g a g t g g g c c c g c a a c a t c c c c t t c t t c c c g g a t c t g c a g a t c a c c g a c c a g g t g c g g c a g c t c a c c c g g g c g t t g t a g g g g a a g a a g g g c c t a g a c g t c t a g t g g c t g g t c c a

4920 VPATPHLERAVRAQRGPVLY SLLRLTWSELFVLNAAQCSM DRSRRVQLSSNTSLAAWHEI g t c c c t g c t a c g c c t c a c c t g g a g c g a g c t g t t c g t g c t c a a c g c g g c c c a g t g c t c t a t c a g g g a c g a t g c g g a g t g g a c c t c g c t c g a c a a g c a c g a g t t g c g c c g g g t c a c g a g a t a

PmlI 4980 AAARGAVAGRRRPACLAHVC PLHVAPLLAAAGLHASPMSA GSCTAGNSAAAPRCAEGMDA g c c g c t g c a c g t g g c g c c g t t g c t g g c c g c c g c c g g c c t g c a t g c c t c g c c c a t g t c t g c c g g c g a c g t g c a c c g c g g c a a c g a c c g g c g g c g g c c g g a c g t a c g g a g c g g g t a c a g a c g

5040 RPRRGLHGPHPHLPGAGGEA DRVVAFMDHIRIFQEQVEKL SRTTAKMSWMRMKWSCTSFS c g a c c g c g t c g t g g c c t t c a t g g a c c a c a t c c g c a t c t t c c a g g a g c a g g t g g a g a a g c t g c t g g c g c a g c a c c g g a a g t a c c t g g t g t a g g c g t a g a a g g t c c t c g t c c a c c t c t t c g a

5100 QGATRRLSRVQLPQSHRAVH KALHVDSAEYSCLKAIVLFT LASCTSEASYLQRLAMTSNV c a a g g c g c t a c a c g t c g a c t c a g c c g a g t a c a g c t g c c t c a a a g c c a t c g t g c t g t t c a c g t t c c g c g a t g t g c a g c t g a g t c g g c t c a t g t c g a c g g a g t t t c g g t a g c a c g a c a a g t g

5160 VRRLWPVGCGPHREPAGEVA SDACGLSDAAHIESLQEKSQ DSAQPRDSAAWMSLRCSFDC g t c a g a c g c c t g t g g c c t g t c g g a t g c g g c c c a c a t c g a g a g c c t g c a g g a g a a g t c g c a c a g t c t g c g g a c a c c g g a c a g c c t a c g c c g g g t g t a g c t c t c g g a c g t c c t c t t c a g c g t

FspAI 5220 VRTGGVREEPVPQPAQPFWQ CALEEYVRSQYPNQPSRFGK HASSSYTLLWYGLWGLRKPL g t g c g c a c t g g a g g a g t a c g t g a g g a g c c a g t a c c c c a a c c a g c c c a g c c g t t t t g g c a a c a c g c g t g a c c t c c t c a t g c a c t c c t c g g t c a t g g g g t t g g t c g g g t c g g c a a a a c c g t t

5280 TAAATALAAHRVLLRHRAAL LLLRLPSLRTVSSSVIEQLF SSSRSGESRVTDEETMSCSK a c t g c t g c t g c g a c t g c c c t c g c t g c g c a c c g t g t c c t c c t c c g t c a t c g a g c a g c t c t t t g a c g a c g a c g c t g a c g g g a g c g a c g c g t g g c a c a g g a g g a g g c a g t a g c t c g t c g a g a a

5340 LRPFGR* FVRLVGKTPIETLIRDMLLS KTRKTPLVGMSVRM c t t c g t c c g t t t g g t a g g t a a a a c c c c c a t c g a a a c t c t c a t c c g c g a t a t g t t a c t g t c g a a g c a g g c a a a c c a t c c a t t t t g g g g g t a g c t t t g a g a g t a g g c g c t a t a c a a t g a c a g

SpeI 5400 GSSFNWPYMSIQCS* t g g g a g c a g c t t c a a c t g g c c t t a c a t g t c c a t c c a g t g c t c c t a g t a a t g a a c t a g t c c a c c c t c g t c g a a g t t g a c c g g a a t g t a c a g g t a g g t c a c g a g g a t c a t t a c t t g a t c a g g

5460 g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a c g t c a t c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t g c a g t a

MluI 5520 WPRE c t a g t g a c a c g c t c g a a c a c g c g t t a a g t c g a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a t c a c t g t g c g a g c t t g t g c g c a a t t c a g c t g t t a g t t g g a g a c c t a a t g t t t t a a a c a

5580 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a

5640 WPRE MPLYHAIASRMAFIFSSLY MLLLPVWLSFSPPCI t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a

5700 WPRE KSWLLSLYEELWPVVRQRGV NPGCCLFMRSCGPLSGNVAW a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c

PflMI 5760 WPRE VCTVFADATPTGWGIATTCQ CALCLLTQPPLVGALPPPVS g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c

5820 WPRE LLSGTFAFPLPIATAELIAA SFPGLSLSPSLLPRRNSSPP c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g

5880 WPRE CLARCWTGARLLGTDNSVVL ALPAAGQGLGCWALTIPWCC t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c

5940 WPRE (in frame with Factor Xa site) *RGKRPQEGTNGGPNQA SGKSSSFPWLLACVATWILR RGNHRPFLGCSPVLPPGFCA t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g

6000 WPRE (in frame with Factor Xa site) PRGEAVDR 1 RGEI Factor Xa site GTSFCYVPSALNPADLPSRG GRPSATSLRPSIQRTFLPAA g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g

6060 WPRE LLPALRPLPRLRLRPQTSRI CCRLCGLFRVFAFALRRVGS c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g

6120 WPRE SLWAASPRRL* PFGPPPRVDFKTNDLQGSCR t c c c t t t g g g c c g c c t c c c c g c g t c g a c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a g g g a a a c c c g g c g g a g g g g c g c a g c t g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c

6180 3' LTR ( Δ U3) S* a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t

6240 3' LTR ( Δ U3) g a c a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g c t g t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c

6300 3' LTR ( Δ U3) a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g

6360 3' LTR ( Δ U3) t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a

SnaBI 6420 3' LTR ( Δ U3) t t t a g t c a g t g t g g a a a a t c t c t a g c a g t a c g t a t a g t a g t t c a t g t c a t c t t a t t a t t c a a a t c a g t c a c a c c t t t t a g a g a t c g t c a t g c a t a t c a t c a a g t a c a g t a g a a t a a t a a g

6480 SV40 poly(A) signal a g t a t t t a t a a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g t c a t a a a t a t t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c

6540 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a

6600 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a

6660 SV40 ori a g c t a t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c g a t a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g

6720 SV40 ori t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g

6780 SV40 ori t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g g a c g t a c c c a a t t c g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c c t g c a t g g g t t a a g c

6840 T7 promoter M13 fwd G G G A T A T C A C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C M13/pUC Forward c c c t a t a g t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g g g a t a t c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c

6900 C M13/pUC Forward g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c

6960 c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g

7020 f1 ori g a a t g g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c c t t a c c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g

7080 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a

7140 f1 ori c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g

7200 f1 ori c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a

7260 f1 ori a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a

7320 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a

7380 f1 ori g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t

7440 f1 ori a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c

7500 AmpR promoter g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g

7560 1 signal sequ... MS AmpR promoter AmpR MS t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t

7620 5 10 15 20 signal sequence IQHFRVALIPFFAAFCLPVF AmpR IQHFRVALIPFFAAFCLPVF t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c

7680 A 25 30 35 40 HPETLVKVKDAEDQLGARV AmpR AHPETLVKVKDAEDQLGARV c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c

7740 45 50 55 60 GYIELDLNSGKILESFRPEE AmpR GYIELDLNSGKILESFRPEE g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g

7800 65 70 75 80 RFPMMSTFKVLLCGAVLSRI AmpR C G A T A C A C C G C G C C A T A A T A Amp-R RFPMMSTFKVLLCGAVLSRI g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c

ScaI 7860 85 90 95 100 DAGQEQLGRRIHYSQNDLVE AmpR DAGQEQLGRRIHYSQNDLVE a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a

7920 105 110 115 120 YSPVTEKHLTDGMTVRELCS AmpR YSPVTEKHLTDGMTVRELCS a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c

7980 125 130 135 140 AAITMSDNTAANLLLTTIGG AmpR AAITMSDNTAANLLLTTIGG c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g

8040 145 150 155 160 PKELTAFLHNMGDHVTRLDR AmpR PKELTAFLHNMGDHVTRLDR c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a

8100 165 170 175 180 WEPELNEAIPNDERDTTMPV AmpR WEPELNEAIPNDERDTTMPV g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c

8160 185 190 195 200 AMATTLRKLLTGELLTLASR AmpR AMATTLRKLLTGELLTLASR c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g

8220 205 210 215 220 QQLIDWMEADKVAGPLLRSA AmpR QQLIDWMEADKVAGPLLRSA a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g

8280 225 230 235 240 LPAGWFIADKSGAGERGSRG AmpR LPAGWFIADKSGAGERGSRG t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t

8340 245 250 255 260 IIAALGPDGKPSRIVVIYTT AmpR IIAALGPDGKPSRIVVIYTT t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c

8400 265 270 275 280 GSQATMDERNRQIAEIGASL AmpR GSQATMDERNRQIAEIGASL g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t

8460 285 IKHW* AmpR IKHW* t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g

8520 t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t

8580 t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a

8640 ori c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g

8700 ori t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c

8760 ori g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g

8820 ori a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c

8880 ori c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c

8940 ori a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t

9000 ori c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c

9060 ori a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t

9120 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a

9180 ori g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t

9240 g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g

9300 c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c

9360 A G C G A G T C A G T G A G C G A G L4440 c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g

9420 c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t

9480 CAP binding site g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g

9540 -35 -10 CAP binding site lac promoter lac operator A M13/pUC Reverse a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t

9600 lac operator M13 rev T3 promoter G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse G C A A T T A T3 g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t

3ʹ 5ʹ 9637 T3 promoter A C C C T C A C T A A A G G T3 a c c c t c a c t a a a g g g a a c a a a a g c t g g a g c t g c a a g c t g g g a g t g a t t t c c c t t g t t t t c g a c c t c g a c g t t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.